CN105675352A - Tear fluid collector - Google Patents

Tear fluid collector Download PDF

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Publication number
CN105675352A
CN105675352A CN201610232017.9A CN201610232017A CN105675352A CN 105675352 A CN105675352 A CN 105675352A CN 201610232017 A CN201610232017 A CN 201610232017A CN 105675352 A CN105675352 A CN 105675352A
Authority
CN
China
Prior art keywords
tear
harvester
sampling
tear fluid
fluid collector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610232017.9A
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Chinese (zh)
Inventor
黄静峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Shengze Kanghua Biological Pharmaceutical Co Ltd
Original Assignee
Guangdong Shengze Kanghua Biological Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Shengze Kanghua Biological Pharmaceutical Co Ltd filed Critical Guangdong Shengze Kanghua Biological Pharmaceutical Co Ltd
Priority to CN201910314758.5A priority Critical patent/CN109883769A/en
Priority to CN201610232017.9A priority patent/CN105675352A/en
Publication of CN105675352A publication Critical patent/CN105675352A/en
Priority to PCT/CN2017/080033 priority patent/WO2017177886A1/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/15Devices for taking samples of blood
    • A61B5/151Devices specially adapted for taking samples of capillary blood, e.g. by lancets, needles or blades
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices

Abstract

The invention belongs to the field of in-vitro diagnosis and particularly relates to a tear fluid collector. The tear fluid collector comprises a collection device, a buffering device and an extrusion device. The rear end of the collection device is connected with the front end of the buffering device, and the front end of the extrusion device is connected with the rear end of the buffering device. Compared with a glass capillary pipette, the tear fluid collector can be better held so that a detector can extrude out a sample conveniently after sampling and collecting. Meanwhile, fear of a patient can be relieved. Compared with a water-absorbing material, the tear fluid collector can be used for accurately quantifying the sample more easily. The technical defect that in the prior art, a tear fluid collector can not take both sampling convenience and quantitative sampling into consideration is overcome. The invention further provides application of the tear fluid collector to in-vitro diagnosis detection and sampling.

Description

A kind of tear harvester
Technical field
The invention belongs to in-vitro diagnosis field, particularly relate to a kind of tear harvester.
Background technology
Containing a large amount of factors and albumen in ocular tear, generation development and molecular mechanism with disease are in close relations, by obtaining tear and carrying out the detection of disease biomarker, to ophthalmic diseases, the especially diagnosis of eye surface diseases, Differential Diagnosis, follow up a case by regular visits to, Outcome measure and prognosis prediction significant.
At present, the tear acquisition method complicated operation used during domestic and international clinical research, and patient often feels nervous, it is impossible to loosen cooperation medical personnel, causes gathering tear or collection effect is undesirable. The tear harvester being currently mainly used specifically includes that glass capillary pipette and two kinds of tear harvesters of water absorbing material. Due to the shape of glass capillary pipette, material and design, have the disadvantage that glass capillary pipette external diameter is many within 1mm, elongated, glass material is to the sharp sensation of people, when looking at capillary glass tube straight, patient is easily generated fear or is nervous, it is impossible to coordinate the operation of examiner preferably; Meanwhile, glass capillary pipette is too thin, and examiner is not easily hand-held and operates; 3rd, the pointed edge of glass capillary pipette, without special handling, and is glass material, comparatively sharp, it is easy to cause epithelial damage; 4th, draw after tear, in order to the blowout of intraluminal liquid, carry out next step inspection, generally also need to the extra device of interim assembling (such as rubber ear washing bulb), the operating procedure of increase operator, operate more complicated. Water absorbing material as the operation principle of tear harvester is: inside the absorbent material contact lower eyelids such as Pilus Caprae seu Ovis terylene (palpebral conjunctiva), the water absorbing properties utilizing material draws tear, subsequently absorbent material is placed in buffer, form the solution containing tear active factors and albumen, then carry out corresponding analysis. The shortcoming that the tear harvester of this type of water absorbing material is primarily present is: first, cannot be quantitative: use absorbent material contact palpebral conjunctiva, absorbent material by tear moistening thus being acquired, but cannot accurately measure the Tear volume drawn, therefore cannot be carried out detection by quantitative or experiment, and affect the result of qualitative test to a certain extent; Second, repetitious stimulation: in order to make absorbent material can draw tear, absorbent material need to be contacted conjunctiva repeatedly, it is possible to cause a large amount of secretion of tear, make ocular tear film composition change; 3rd, patient is uncomfortable: ocular is distributed a large amount of teleneurons, and absorbent material contacts conjunctiva repeatedly, and patient can be caused uncomfortable; 4th, the sample of collection is diluted: tear is adsorbed in absorbent material, therefore needs to be placed in buffer by absorbent material, after tear protein and active factors are dissolved in buffer, can be analyzed. The tear that this process can cause sample size own minimum is diluted so that original concentration is more difficult to be detected with regard to low target analyte, thus limiting its clinical practice. Therefore, tear harvester of the prior art, it is primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling.
Therefore, develop a kind of tear harvester, be used for solving tear harvester of the prior art, be primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling, become those skilled in the art's problem demanding prompt solution.
Summary of the invention
In view of this, the invention provides a kind of tear harvester, be used for solving in prior art, tear harvester is primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling.
The invention provides a kind of tear harvester, described tear harvester includes: harvester, buffer unit and pressurizing unit; The rear end of described harvester is connected with the front end of described buffer unit, and the front end of described pressurizing unit is connected with the rear end of described buffer unit.
Preferably, described tear harvester also includes: sealing device; Described sealing device is enclosed within described harvester and the outside of described buffer unit.
Preferably, the rear end of described pressurizing unit or upper end are provided with cavernous structure.
Preferably, described harvester is capillary pipette.
Preferably, described buffer unit be shaped as beloid pyramidal structure.
Preferably, described pressurizing unit is elastic squeezing unit.
Preferably, described harvester is transparent harvester.
Preferably, described buffer unit is transparent caching device.
Present invention also offers a kind of application included in the tear harvester diagnostic detection in vitro sampling that any of the above one is described.
In sum, the invention provides a kind of tear harvester, described tear harvester includes: harvester, buffer unit and pressurizing unit; The rear end of described harvester is connected with the front end of described buffer unit, and the front end of described pressurizing unit is connected with the rear end of described buffer unit. A kind of tear harvester provided by the invention, utilizes harvester to collect tear, when tear arrives the junction of harvester and buffer unit, sample complete, when tear is detected by needs, can pass through to extrude pressurizing unit so that tear discharges tear harvester. Compared with glass capillary pipette, tear harvester provided by the invention better grips is easy to testing staff's sampling, simultaneously, it is possible to alleviate the fear of patient. Compared with water absorbing material, tear harvester provided by the invention is easier to sample is carried out accurate quantitative analysis. Solving in prior art, tear harvester is primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling. Present invention also offers the application in above-mentioned tear harvester diagnostic detection in vitro sampling.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below, apparently, accompanying drawing in the following describes is only embodiments of the invention, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to the accompanying drawing provided.
Fig. 1 is one of them structural representation of a kind of tear harvester provided by the invention;
Fig. 2 is another structural representation of a kind of tear harvester provided by the invention;
Wherein, harvester 1, buffer unit 2, pressurizing unit 3, sealing device 4 and cavernous structure 31.
Detailed description of the invention
The invention provides a kind of tear harvester, be used for solving tear harvester of the prior art, be primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling.
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments. Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.
In order to be described in more detail the present invention, below in conjunction with embodiment to a kind of tear harvester provided by the invention, it is specifically described.
Refer to Fig. 1 and Fig. 2, the invention provides a kind of tear harvester, including: harvester 1, buffer unit 2 and pressurizing unit 3; The rear end of harvester 1 is connected with 2 front ends of buffer unit, and the front end of buffer unit 3 is connected with the rear end of buffer unit 1. . A kind of tear harvester provided by the invention, harvester 1 is utilized to collect tear, when tear arrives the junction of harvester 1 and buffer unit 2, sample complete, after utilizing harvester 1 to collect tear, when tear is detected by needs, pressurizing unit 3 can be extruded so that tear discharges tear harvester. Compared with glass capillary pipette, tear harvester provided by the invention better grips is easy to testing staff's sampling, simultaneously, it is possible to alleviate the fear of patient. Compared with water absorbing material, tear harvester provided by the invention is easier to sample is carried out accurate quantitative analysis. Solving in prior art, tear harvester is primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling.
In order to ensure that harvester is aseptic and prevent the ocular fluid samples collected contaminated, a kind of tear harvester provided by the invention also includes: sealing device 4; Sealing device 4 is enclosed within harvester 1 and the outside of buffer unit 2. Meanwhile, before tear harvester does not also use, sealing device 4 can so that the tip of harvester 1 be not subjected to the damage polluted and collision brings.
Quickly drawing the sample of determined volume in order to tear harvester can pass through siphon capillarity, the rear end of the pressurizing unit 3 of a kind of tear harvester provided by the invention is provided with cavernous structure 31. After tear harvester is collected tear, for the ease of the discharge of tear, in order to carrying out next step detection, the pressurizing unit 3 of a kind of tear harvester provided by the invention has elasticity and can be extruded. After sample collecting, first sample is mixed with buffer if needed, harvester 1 can be inserted in buffer pipe, block cavernous structure 31 with finger, extrude pressurizing unit 3, sample is blown in buffer pipe, draw buffer again through release pressure and enter in harvester 1, and then block cavernous structure 31 with finger, and extrude pressurizing unit 3 simultaneously, liquid is blown out. After sample collecting, it is also possible to by blocking cavernous structure 31 with finger, extrude pressurizing unit 3, directly sample is blown into the well in detecting device.
For ease of sampling, in the embodiment of the present invention, harvester 1 is capillary pipette. Utilize capillarity (that is: the liquid surface captivation to the surface of solids) imbitition. When capillary pipette inserts in immersion liquid, intraluminal fluid face rises, outside higher than pipe. Liquid level can be examined by collection head transparent tube standing motionless as if facing a wall, sample when capillary pipette is hydraulically full complete.
For realizing accurate quantitative analysis sampling, in the embodiment of the present invention, harvester 1 be shaped as the front fine and rear thick funnel structure that capillary pipette rear portion, front end caliber strengthens rapidly, the rear end of pressurizing unit 3 is provided with cavernous structure 31 simultaneously. The internal diameter of capillary pipette and length determine the liquid volume drawn, i.e. collection capacity. When ocular fluid samples is full of harvester 1, i.e. capillary pipette, namely siphon capillarity stops.
Try one's best on the short and small convenient basis gripped at tear harvester, meet multi-level user demand, it is achieved the collection capacity of the volume tear of precise quantitative, in the embodiment of the present invention, buffer unit 2 be shaped as beloid pyramidal structure. The design of pyramidal structure, not only convenient gripping, and volume is little and mellow and full, can alleviate the fear of patient while convenient use.
Discharge after gathering for the ease of tear, in the embodiment of the present invention, pressurizing unit 3 is elastic squeezing unit. Use only small power just can realize the discharge of tear, simple operation.
For ease of observing the collection situation of tear, in the embodiment of the present invention, harvester 1 is transparent harvester, and buffer unit 2 is transparent caching device.
Present invention also offers the application in a kind of tear harvester diagnostic detection in vitro sampling including any of the above one. The above-mentioned embodiment that provide only collection tear, those skilled in the art can clearly draw, under the premise not paying creative work, a kind of tear harvester provided by the invention can be used for the collecting work of the various body fluid such as saliva, urine, blood.
In brief, a kind of tear harvester provided by the invention, have the following advantages. First, safety: the structure of a kind of tear harvester provided by the invention is more mellow and fuller, make the comparatively round and smooth finishing in the position with patient contact, under normal operating, noinvasive is painless, produces infringement without to eyeball ocular. Second, ease for operation: a kind of tear harvester provided by the invention designs according to principle of human engineering, makes the length of harvester, diameter be suitable for hand-held and do accurate operation in ocular. 3rd, sample rapid and simple, a kind of tear harvester design provided by the invention applies capillary siphoning action principle, and there is cavernous structure the rear end that harvester is capillary tube that the smooth rounding of surfaces externally and internally, internal diameter are tiny and pressurizing unit, and tear can quickly enter harvester. 4th, quantitative sampling: utilize the internal diameter of harvester and length accurately to control to suck the volume of liquid, establish good basis for the concentration of biomarker in quantitative analysis tear. 5th, sampling sample presentation integration: after tear harvester gathers liquid, generally need to be transported on suitable verifying attachment and (such as gold mark card etc.) or instrument and equipment are tested, the tear harvester of present invention design itself carries after having elastic extruding and manages, tear in harvester and apparatus for temporary storage can be blown out, it is achieved sampling sample presentation integration. 6th, improve those who are investigated's experience: mellow and full full in harvester industrial design, to patient with the security; Utilize fine mould, make the collection head similar with glass capillary straw diameter most advanced and sophisticated, not in contact with (or inconspicuous stimulation) ocular tissue, it is achieved noinvasive is painless.
By above description it should be apparent that a kind of tear harvester provided by the invention, solving in prior art, tear harvester is primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling. meanwhile, in clinical practice and industry application aspect, all it is significant. in clinical value, as the body fluid class biological sample that the unique noinvasive of ophthalmology is desirable, containing a large amount of factors and albumen in ocular tear, generation development and molecular mechanism with disease are in close relations, but it is because on market or does not still facilitate clinically, safety, effective tear harvester or method, the collection of ocular fluid samples and analysis are only limitted to minority professional scientific researcher and scientific research character, this is one of key reason of external diagnosis reagent always lacking detection tear, various biochemistrys, protein analysis detection technique or immunologic detection method all not can apply in the diagnosis of ophthalmic diseases. a kind of tear harvester provided by the invention, has convenience, safe and effective advantage. meanwhile, the presence or absence of various active factorses in tear, protein factor and biomarker or its concentration, react health and the pathological state of ocular. after gathering the tear of specified disease patient, the measurement of its composition, certain biomarker factor or protein factor is analyzed, is conducive to studying the Molecular Pathogcnesis of this disease. by obtain tear detect, to ophthalmic diseases, the especially diagnosis of eye surface diseases, Differential Diagnosis, follow up a case by regular visits to, Outcome measure and prognosis prediction significant. in industry using value, compared with prior art, namely glass capillary pipette is compared with water absorbing material, a kind of tear harvester provided by the invention can quantitatively, in precision, accuracy, ease for operation, convenience and user experience, it is obviously improved, probability and practicality will be provided for clinical tear collection, promote development and the ophthalmology external diagnosis reagent product development and application of tear correlation detection technology, and strengthen ophthalmic diseases and precisely diagnose and treat.
In sum, the invention provides a kind of tear harvester, including: harvester 1, buffer unit 2 and pressurizing unit 3; The rear end of harvester 1 is connected with 2 front ends of buffer unit, and the front end of buffer unit 3 is connected with the rear end of apparatus for temporary storage 1. . A kind of tear harvester provided by the invention, harvester 1 is utilized to collect tear, when tear arrives the junction of harvester 1 and buffer unit 2, sample complete, after utilizing harvester 1 to collect tear, when tear is detected by needs, pressurizing unit 3 can be extruded so that tear discharges tear harvester. Compared with glass capillary pipette, tear harvester provided by the invention better grips is easy to testing staff's sampling, simultaneously, it is possible to alleviate the fear of patient. Compared with water absorbing material, tear harvester provided by the invention is easier to sample is carried out accurate quantitative analysis. Solving in prior art, tear harvester is primarily present the technological deficiency can not simultaneously taken into account between convenience and quantitative sampling of sampling. Present invention also offers the application in above-mentioned tear harvester diagnostic detection in vitro sampling.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention; can also making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.

Claims (9)

1. a tear harvester, it is characterised in that described tear harvester includes: harvester, buffer unit and pressurizing unit;
The rear end of described harvester is connected with the front end of described buffer unit, and the front end of described pressurizing unit is connected with the rear end of described buffer unit.
2. tear harvester according to claim 1, it is characterised in that described tear harvester also includes: sealing device;
Described sealing device is enclosed within described harvester and the outside of described buffer unit.
3. tear harvester according to claim 1, it is characterised in that rear end or the upper end of described pressurizing unit are provided with cavernous structure.
4. tear harvester according to claim 1, it is characterised in that described harvester is capillary pipette.
5. tear harvester according to claim 1, it is characterised in that described buffer unit be shaped as beloid pyramidal structure.
6. tear harvester according to claim 1, it is characterised in that described pressurizing unit is elastic squeezing unit.
7. tear harvester according to claim 1, it is characterised in that described harvester is transparent harvester.
8. tear harvester according to claim 1, it is characterised in that described buffer unit is transparent caching device.
9. the application in the tear harvester diagnostic detection in vitro sampling that a kind includes described in claim 1 to 8 any one.
CN201610232017.9A 2016-04-13 2016-04-13 Tear fluid collector Pending CN105675352A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201910314758.5A CN109883769A (en) 2016-04-13 2016-04-13 A kind of tear quantitative collector
CN201610232017.9A CN105675352A (en) 2016-04-13 2016-04-13 Tear fluid collector
PCT/CN2017/080033 WO2017177886A1 (en) 2016-04-13 2017-04-11 Tear collector

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Application Number Priority Date Filing Date Title
CN201610232017.9A CN105675352A (en) 2016-04-13 2016-04-13 Tear fluid collector

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CN201910314758.5A Pending CN109883769A (en) 2016-04-13 2016-04-13 A kind of tear quantitative collector

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WO (1) WO2017177886A1 (en)

Cited By (3)

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CN106419831A (en) * 2016-07-22 2017-02-22 广州市眼视光健康产业投资管理有限公司 Body health monitoring glasses
WO2017177886A1 (en) * 2016-04-13 2017-10-19 广东盛泽康华生物医药有限公司 Tear collector
CN108152087A (en) * 2018-01-03 2018-06-12 沈阳何氏眼科医院有限公司 A kind of tear remote collection for being used to diagnose dry eyes and analytical equipment and analysis method

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EP3779397A4 (en) * 2018-03-30 2021-12-15 Provigate Inc. Trace amount liquid sample collector

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CN205785930U (en) * 2016-04-13 2016-12-07 广东盛泽康华生物医药有限公司 A kind of tear harvester

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US4589421A (en) * 1984-03-14 1986-05-20 Syntex (U.S.A.) Inc. Sampling device
CN1969184A (en) * 2004-04-16 2007-05-23 拉希达·A·卡马利 Specimen collecting, processing and analytical assembly
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017177886A1 (en) * 2016-04-13 2017-10-19 广东盛泽康华生物医药有限公司 Tear collector
CN106419831A (en) * 2016-07-22 2017-02-22 广州市眼视光健康产业投资管理有限公司 Body health monitoring glasses
CN108152087A (en) * 2018-01-03 2018-06-12 沈阳何氏眼科医院有限公司 A kind of tear remote collection for being used to diagnose dry eyes and analytical equipment and analysis method
CN108152087B (en) * 2018-01-03 2023-04-14 沈阳何氏眼科医院有限公司 Tear remote acquisition and analysis device and analysis method for diagnosing dry eyes

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Publication number Publication date
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WO2017177886A1 (en) 2017-10-19

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Application publication date: 20160615