CN102532610A - Pathological embedding agent, preparation method and applications thereof - Google Patents
Pathological embedding agent, preparation method and applications thereof Download PDFInfo
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- CN102532610A CN102532610A CN2011104400141A CN201110440014A CN102532610A CN 102532610 A CN102532610 A CN 102532610A CN 2011104400141 A CN2011104400141 A CN 2011104400141A CN 201110440014 A CN201110440014 A CN 201110440014A CN 102532610 A CN102532610 A CN 102532610A
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Abstract
The invention discloses a pathological embedding agent, a preparation method and an application thereof. The pathological embedding agent is characterized in that the pathological embedding agent comprises, by weight, 1-90% of pre-gelatinized starch and 10-99% of gelatin powder or/and paraffin powder. The preparation method is: weighing the components according to the weight percentages in the formula; placing the components into a clean container, uniformly stirring and sealed packaging to obtain the pathological embedding agent. The invention provides the application of the pathological embedding agent in cell specimen capture or pathological fine tissue specimen capture or paraffin embedding slice. The purposes of the invention are that: the pathological embedding agent capable of effective capture of cells attached to the container wall is provided, and the pathological embedding slice capable of permanent preservation is prepared through the pathological embedding agent; the method for preparing the pathological embedding agent is provided; the prepared pathological embedding agent is adopted as the capture agent for the cell specimens and the pathological fine tissue specimens, and the application of the pathological embedding agent in the preparation of the paraffin embedding slice is provided.
Description
Technical field
The present invention relates to a kind of pathology embedding medium, the invention still further relates to a kind of method for preparing this pathology embedding medium; The invention still further relates to the application of this pathology embedding medium.
Background technology
All carried out at present the inspection of cast-off cells such as cervical exfoliated cell, ascites pleural fluid, sputum, urine, puncture fluid, cerebrospinal fluid, puncture fluid in national various big hospital Pathology Deparment.The step of inspection is, get above sample and put into sample and preserve that liquid is fixing to be preserved, with the fixed sample put into centrifuge tube, centrifugal on whizzer; Supernatant inclines; Get sample in the centrifuge tube, film-making, dyeing, covered are at the microscopically microscopy; Patient's patient's condition, focus etc. are judged in the variation of states such as observation of cell nuclear, cytoplasm, cell walls.The doctor of Pathology Deparment be often because of the cell concentration that adheres to slide can't provide judgement very little in this process, at this moment from centrifuge tube, getting sample more again during film-making, can't grasp the cell that is attached to centrifugal tube wall again.Next two kinds of phenomenons are arranged usually: one, Pathology Deparment's announcement of directly transmitting messages; Two, Pathology Deparment requires the clinician to draw materials once more, again inspection.The former fails to pinpoint a disease in diagnosis, and probability is bigger, and the latter lets the patient go out expense again one time, and misery once particularly punctures and draws materials again, and the patient is more painful.Sometimes due to illness patient physiological factor reason is drawn materials once more and also can't be reached the cell of q.s.Like the cervical exfoliated cell sampling, because self is metabolic slow, the cast-off cells amount is less, the also requirement of the amount of not reaching of drawing materials once more in high year women.Through statistics; Traditional method can't be taken out the cell specimen that is attached to centrifugal tube wall, and it accounts for the specimen amount 1/8 to 2/3 of drawing materials, and often the ability of the cell adhesion tube wall of pathology is stronger; Cell concentration is few more in the sampling sample, and the shared ratio of cell that is attached to centrifugal tube wall is big more.
Thereby need a kind of technological method that substitutes above weak point, and grasp the cell that is attached to centrifugal tube wall as far as possible, prevent the cell loss of pathology, help the pathology expert and make judgement accurately, be the important means of avoiding patient's condition omission.
Conventional cell specimen preparation, sample sheet generally by all means 3-6 month, later with oxidation stain gradually, thereby the also needs method that can preserve cast-off cells film-making sample for a long time.
Summary of the invention
The objective of the invention is in order to overcome weak point of the prior art, provide a kind of and can effectively catch the pathology embedding medium that is attached to cell on the vessel wall, and can be made into the pathology embedding sheet of permanent preservation through this pathology embedding medium;
Another object of the present invention provides a kind of method for preparing the pathology embedding medium;
The object of the invention also have one be with preparation pathology embedding medium as cell specimen, pathology tissue sample trapping agent in small, broken bits, and the application in preparation paraffin embedding film-making.
In order to achieve the above object, the present invention adopts following scheme:
A kind of pathology embedding medium is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 1%-90%
Jelly powder is or/and paraffin powder 10%-99%.
Aforesaid a kind of pathology embedding medium is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 50%-90%
Paraffin powder 10%-50%.
Aforesaid a kind of pathology embedding medium is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 50%-90%
Jelly powder 10%-50%.
Aforesaid a kind of pathology embedding medium is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 1%-90%
Jelly powder 5%-50%
Paraffin powder 5%-50%.
Arbitrary pathology embedding medium in a kind of preparation of the present invention claim 1 to 4 is characterized in that may further comprise the steps:
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
The application of pathology embedding medium of the present invention on cell specimen is caught.
The application that pathology embedding medium of the present invention is caught at pathology tissue sample in small, broken bits.
Pathology embedding medium of the present invention is in the application of making in the paraffin embedding film-making.
The Pathologic specimen that can catch through the pathology embedding medium among the present invention is made pathology paraffin embedding, frozen section, film-making, and cell specimen is preserved for a long time.
The character of composition is following in the present invention's prescription:
Pre-gelatinized starch: pre-gelatinized starch is that a kind of processing is simple, and broad-spectrum modified starch as long as stick with paste with the cold water furnishing, has been exempted the trouble of heating gelatinization during application.Widespread use and a lot of industries such as medicine, food, makeup, feed, petroleum drilling, metal casting, weaving, papermaking.
The unit that the pre-gelatinized starch composition constitutes starch and dextrin all is a glucose; It is more than dextrin that but starch contains the number of glucose unit; Starch pasting is actually the incomplete hydrolysis of starch, and the product of starch complete hydrolysis is a glucose, and SANMALT-S also can be the not intact hydrolysate of starch; But SANMALT-S only contains two glucose units, and the glucose unit that contains than dextrin lacks.
Selecting the pre-gelatinized starch condition among the present invention is that the 2g pre-gelatinized starch is put into 10ml zero(ppm) water mixing, leave standstill 15-20 minute after, it is as clear as crystal to observe the water surface; There is deposition at the bottom, and this pre-gelatinized starch is best, and it promptly has adhesivity; Tool is water insoluble again, does not influence the cell transparency.
Jelly powder: water soluble protein mixture; Collagen in skin, ligament, the tendon is through acid or alkali partly hydrolysed or in water, boil and produce; Crisp or meal shape that colourless or little Huang is transparent, swelling forms gel in 35～40 ℃ of water, and is moisture for conducting oneself with dignity 5～10 times.Be the nutrition incomplete protein, lack some indispensable amino acid, especially tryptophane is widely used in food and makes tamanori, photographic film, spectral filter etc.It is colourless, transparent that the present invention program selects, and can do the jelly powder of sensitive film, and it has adhesion, transparent characteristic does not influence the sight sheet.
Paraffin powder: select pathological section paraffin, process powder.It has high light transmittance.
In sum, beneficial effect of the present invention:
Pathology embedding medium of the present invention can be caught the cell specimen that sticks to vessel wall effectively, again through paraffin embedding, and frozen section, dyeing and process sheet and can forever preserve.With respect to the conventional cell specimen preparation: be coated in cell on the slide, after the dyeing, covered, general this method cell specimen was preserved 3-6 month.Retention period is longer, better effects if.
Embodiment
In order to understand the present invention better, come the present invention is described further below in conjunction with specific embodiment, but the present invention's scope required for protection is not limited to the scope that embodiment puts down in writing.
Embodiment 1
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 1%;
Jelly powder 99%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 2
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 90%;
Jelly powder 10%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 3
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 50%;
Jelly powder 50%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 4
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 60%;
Jelly powder 40%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 5
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 5%;
Paraffin powder 95%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 6
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 85%;
Paraffin powder 15%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 7
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 20%;
Paraffin powder 80%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 8
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 30%;
Paraffin powder 70%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 9
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 65%;
Paraffin powder 35%;
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 10
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 1%;
Jelly powder 50%;
Paraffin powder 49%.
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 11
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 46%;
Jelly powder 27%;
Paraffin powder 27%.
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Embodiment 12
Pathology embedding medium of the present invention, form by following components in weight percentage:
Pre-gelatinized starch 30%
Jelly powder 35%
Paraffin powder 35%.
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
Adopt pathology embedding medium of the present invention to carry out clinical trial:
Clinical trial one:
Subjects:
Liu: woman; Age: 55 years old;
Inspection item: examination (TCT) before the cervical cancer
Inspection hospital: Zhongshan city port hospital
Agents useful for same:
Pathology embedding medium of the present invention.
Simultaneous test:
1, draw materials for the first time, sampling put in the centrifuge tube centrifugal after, the supernatant that inclines is got specimen preparation, dyeing in the pipe, microscopy, cell concentration is few, can't draw a conclusion or does not note abnormalities cell.
2, draw materials for the second time, that sampling is put is centrifugal in the centrifuge tube, film-making, dyeing, microscopy, and cell concentration is few, can't draw a conclusion or does not note abnormalities cell.
3, draw materials for the third time, centrifugal in the centrifuge tube, the supernatant that inclines is put in sampling, with half spoonful of pathology embedding medium of the present invention, inserts in the centrifuge tube, and cotton swab stirs, and takes out sample, carries out paraffin embedding, the section of freezing back, film-making dyeing, microscopy, conclusion CINI level.
Clinical trial two:
Subjects:
The Wang, the woman; Age: 45 years old;
Inspection item: examination (TCT) before the cervical cancer
Inspection hospital: the Chen Xing of Zhongshan city sea hospital
Agents useful for same: pathology embedding medium of the present invention.
Simultaneous test:
1, draw materials, sampling put in the centrifuge tube centrifugal after, the supernatant that inclines is got specimen preparation dyeing in the pipe, microscopy, cell concentration is few, difficulty is drawn a conclusion.
2, put in the centrifuge tube for 1/3 spoonful with pathology embedding medium of the present invention, cotton swab stirs, and takes out sample, carries out paraffin embedding, the section of freezing back, film-making dyeing, microscopy, conclusion HSIL.
Pathology embedding medium of the present invention not only can be used in the catching of cell specimen, paraffin embedding film-making, also can be used on the catching of pathology tissue sample in small, broken bits, paraffin embedding film-making.
Claims (8)
1. pathology embedding medium is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 1%-90%
Jelly powder is or/and paraffin powder 1%-99%.
2. a kind of pathology embedding medium according to claim 1 is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 50%-90%
Paraffin powder 10%-50%.
3. a kind of pathology embedding medium according to claim 1 is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 50%-90%
Jelly powder 10%-50%.
4. a kind of pathology embedding medium according to claim 1 is characterized in that being made up of following components in weight percentage:
Pre-gelatinized starch 1%-90%
Jelly powder 5%-50%
Paraffin powder 5%-50%.
5. one kind prepares arbitrary pathology embedding medium in the claim 1 to 4, it is characterized in that may further comprise the steps:
Weight percent by in the prescription takes by weighing each component, puts into clean container then and mixes encapsulation thoroughly and can obtain the pathology embedding medium.
6. the application of arbitrary pathology embedding medium on cell specimen is caught in the claim 1 to 4.
7. the application that arbitrary pathology embedding medium is caught at pathology tissue sample in small, broken bits in the claim 1 to 4.
8. the application of arbitrary pathology embedding medium in the film-making of making paraffin embedding in the claim 1 to 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201110440014.1A CN102532610B (en) | 2011-12-23 | 2011-12-23 | Pathological embedding agent, preparation method and applications thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110440014.1A CN102532610B (en) | 2011-12-23 | 2011-12-23 | Pathological embedding agent, preparation method and applications thereof |
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| Publication Number | Publication Date |
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| CN102532610A true CN102532610A (en) | 2012-07-04 |
| CN102532610B CN102532610B (en) | 2014-03-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106501057A (en) * | 2016-03-29 | 2017-03-15 | 吴菡 | A kind of paraffin-embedded improving technology of cell specimen |
Citations (3)
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| US20040048896A1 (en) * | 1996-01-04 | 2004-03-11 | Phillips Jeffrey Owen | Novel substituted benzimidazole dosage forms and method of using same |
| CN101248883A (en) * | 2008-04-01 | 2008-08-27 | 吉林大学 | Egg white high F values oligopeptide buccal tablets and method of preparing the same |
| CN101326937A (en) * | 2008-08-04 | 2008-12-24 | 天津科技大学 | Method for preparing concentrated cheese powder by accelerated fermentation |
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2011
- 2011-12-23 CN CN201110440014.1A patent/CN102532610B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040048896A1 (en) * | 1996-01-04 | 2004-03-11 | Phillips Jeffrey Owen | Novel substituted benzimidazole dosage forms and method of using same |
| CN101248883A (en) * | 2008-04-01 | 2008-08-27 | 吉林大学 | Egg white high F values oligopeptide buccal tablets and method of preparing the same |
| CN101326937A (en) * | 2008-08-04 | 2008-12-24 | 天津科技大学 | Method for preparing concentrated cheese powder by accelerated fermentation |
Non-Patent Citations (1)
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| 孙梅等: "酪酸菌的微胶囊化研究", 《畜牧与饲料科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106501057A (en) * | 2016-03-29 | 2017-03-15 | 吴菡 | A kind of paraffin-embedded improving technology of cell specimen |
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| CN102532610B (en) | 2014-03-12 |
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Address after: Room 106, building e, junxianju, 328 Changjiang North Road, East District, Zhongshan City, Guangdong Province, 528400 Patentee after: Guangdong Kangnaixin Biomedical Technology Co.,Ltd. Address before: Room 106, building e, junxianju, 328 Changjiang North Road, East District, Zhongshan City, Guangdong Province, 528400 Patentee before: KANG NAI XIN ZHONGSHAN BIOTECHNOLOGY Co.,Ltd. |
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Address after: 528437 whole floor, 2nd floor, No.1 Guangji West Road, Torch Development Zone, Zhongshan City, Guangdong Province Patentee after: Guangdong Kangnaixin Biomedical Technology Co.,Ltd. Address before: Room 106, building e, junxianju, 328 Changjiang North Road, East District, Zhongshan City, Guangdong Province, 528400 Patentee before: Guangdong Kangnaixin Biomedical Technology Co.,Ltd. |