CN102532610B - Pathological embedding agent, preparation method and applications thereof - Google Patents
Pathological embedding agent, preparation method and applications thereof Download PDFInfo
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- CN102532610B CN102532610B CN201110440014.1A CN201110440014A CN102532610B CN 102532610 B CN102532610 B CN 102532610B CN 201110440014 A CN201110440014 A CN 201110440014A CN 102532610 B CN102532610 B CN 102532610B
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Abstract
The invention discloses a pathological embedding agent, a preparation method and an application thereof. The pathological embedding agent is characterized in that the pathological embedding agent comprises, by weight, 1-90% of pre-gelatinized starch and 10-99% of gelatin powder or/and paraffin powder. The preparation method is: weighing the components according to the weight percentages in the formula; placing the components into a clean container, uniformly stirring and sealed packaging to obtain the pathological embedding agent. The invention provides the application of the pathological embedding agent in cell specimen capture or pathological fine tissue specimen capture or paraffin embedding slice. The purposes of the invention are that: the pathological embedding agent capable of effective capture of cells attached to the container wall is provided, and the pathological embedding slice capable of permanent preservation is prepared through the pathological embedding agent; the method for preparing the pathological embedding agent is provided; the prepared pathological embedding agent is adopted as the capture agent for the cell specimens and the pathological fine tissue specimens, and the application of the pathological embedding agent in the preparation of the paraffin embedding slice is provided.
Description
Technical field
The present invention relates to a kind of pathology embedding medium, the invention still further relates to a kind of method of preparing this pathology embedding medium; The invention still further relates to the application of this pathology embedding medium.
Background technology
In national various big hospital Pathology Deparment, all carried out at present the inspection of the cast-off cells such as cervical exfoliated cell, ascites pleural fluid, sputum, urine, puncture fluid, cerebrospinal fluid, puncture fluid.The step checking is, get above sample and put into the fixing preservation of specimen preserving liquid, by fixing sample put into centrifuge tube, centrifugal on whizzer, supernatant liquor inclines, get sample in centrifuge tube, film-making, dyeing, covered, under the microscope microscopy, the variation of the states such as observation of cell core, cytoplasm, cell walls, judges patient's patient's condition, focus etc.In this process, pathologists often cannot provide judgement very little because adhering to the cell concentration of slide, at this moment again from centrifuge tube label taking originally again during film-making, cannot capture the cell that is attached to centrifugal tube wall again.Next conventionally there are two kinds of phenomenons: the Yi, Pathology Deparment announcement of directly transmitting messages; Er, Pathology Deparment requires clinician again to draw materials, then examines.The former fails to pinpoint a disease in diagnosis, and probability is larger, and the latter allows patient go out expense one time again, then misery once, and particularly puncture is drawn materials, and patient is more painful.Sometimes due to illness patient physiological factor reason is again drawn materials and also cannot be reached the cell of q.s.As cervical exfoliated cell sampling, in high year women, because self is metabolic slow, cast-off cells amount is less, the also requirement of the amount of not reaching of again drawing materials.Through statistics, traditional method cannot be taken out the cell specimen that is attached to centrifugal tube wall, and it accounts for the specimen amount 1/8 to 2/3 of drawing materials, and often the ability of the cell adhesion tube wall of pathology is stronger, in sampling sample, cell concentration is fewer, and the shared ratio of cell that is attached to centrifugal tube wall is larger.
Thereby need a kind of technological method that substitutes above weak point, and capture the cell that is attached to centrifugal tube wall as far as possible, prevent the cell loss of pathology, be conducive to pathology expert and make judgement accurately, be the important means of avoiding the patient's condition undetected.
Conventional cell specimen preparation, sample slice generally by all means 3-6 month, later by oxidation stain gradually, thereby the method that also needs to preserve for a long time cast-off cells film-making sample.
Summary of the invention
The object of the invention is in order to overcome weak point of the prior art, provide a kind of and can effectively catch the pathology embedding medium that is attached to cell on vessel wall, and can be made into by this pathology embedding medium the pathology embedding sheet of persistence;
Another object of the present invention is to provide a kind of method of preparing pathology embedding medium;
Object of the present invention also has one to be to prepare pathology embedding medium as cell specimen, pathology tissue sample trapping agent in small, broken bits, and in the application of preparing in paraffin embedding film-making.
In order to achieve the above object, the present invention adopts following scheme:
A pathology embedding medium, is characterized in that being comprised of the component of following weight percent:
Pre-gelatinized starch 1%-90%
Jelly powder is or/and paraffin powder 10%-99%.
A kind of pathology embedding medium as above, is characterized in that being comprised of the component of following weight percent:
Pre-gelatinized starch 50%-90%
Paraffin powder 10%-50%.
A kind of pathology embedding medium as above, is characterized in that being comprised of the component of following weight percent:
Pre-gelatinized starch 50%-90%
Jelly powder 10%-50%.
A kind of pathology embedding medium as above, is characterized in that being comprised of the component of following weight percent:
Pre-gelatinized starch 1%-90%
Jelly powder 5%-50%
Paraffin powder 5%-50%.
The present invention is a kind of prepares arbitrary pathology embedding medium in claim 1 to 4, it is characterized in that comprising the following steps:
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
The application of pathology embedding medium of the present invention on cell specimen is caught.
The application that pathology embedding medium of the present invention is caught at pathology tissue sample in small, broken bits.
Pathology embedding medium of the present invention is in the application of making in paraffin embedding film-making.
The Pathologic specimen that can catch by pathology embedding medium in the present invention, makes pathology paraffin embedding, frozen section, film-making, and cell specimen is preserved for a long time.
In the present invention's formula, the character of composition is as follows:
Pre-gelatinized starch: pre-gelatinized starch is that a kind of processing is simple, broad-spectrum modified starch, as long as stick with paste with cold water furnishing, has exempted the trouble of heating gelatinization during application.Widespread use and a lot of industries such as medicine, food, makeup, feed, petroleum drilling, metal casting, weaving, papermaking.
The unit that pre-gelatinized starch composition forms starch and dextrin is all glucose, but the number that starch contains glucose unit is more than dextrin, starch pasting is actually the incomplete hydrolysis of starch, the product of starch complete hydrolysis is glucose, maltose can be also the not complete hydrolysate of starch, but maltose is only containing two glucose units, and the glucose unit containing than dextrin is few.
In the present invention, selecting pre-gelatinized starch condition is 2g pre-gelatinized starch to be put into 10ml distilled water mix, and after standing 15-20 minute, observes the water surface as clear as crystal, there is precipitation at the bottom, and this pre-gelatinized starch is best, and it have adhesivity, tool is water insoluble again, does not affect the cell transparency.
Jelly powder: water soluble protein mixture, collagen in skin, ligament, tendon is through acid or alkali partial hydrolysis or in water, boil and produce, crisp or meal shape that colourless or micro-Huang is transparent, in 35～40 ℃ of water, swelling forms gel, and moisture is 5～10 times of deadweights.Be nutrition incomplete protein, lack some indispensable amino acid, especially tryptophane, is widely used in food and makes tamanori, photographic film, spectral filter etc.It is colourless, transparent that the present invention program selects, and can do the jelly powder of sensitive film, and it has adhesion, transparent characteristic does not affect sight sheet.
Paraffin powder: select pathological section paraffin, make powder.It has high light transmittance.
In sum, beneficial effect of the present invention:
Pathology embedding medium of the present invention can be caught the cell specimen that sticks to vessel wall effectively, then by paraffin embedding, frozen section, dyeing and make sheet can persistence.With respect to conventional cell specimen preparation: cell is coated on slide, after dyeing, covered, general this method cell specimen is preserved 3-6 month.Retention period is longer, better effects if.
Embodiment
In order to understand better the present invention, below in conjunction with specific embodiment, the invention will be further described, but the present invention's scope required for protection is not limited to the scope that embodiment records.
Embodiment 1
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 1%;
Jelly powder 99%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 2
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 90%;
Jelly powder 10%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 3
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 50%;
Jelly powder 50%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 4
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 60%;
Jelly powder 40%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 5
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 5%;
Paraffin powder 95%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 6
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 85%;
Paraffin powder 15%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 7
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 20%;
Paraffin powder 80%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 8
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 30%;
Paraffin powder 70%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 9
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 65%;
Paraffin powder 35%;
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 10
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 1%;
Jelly powder 50%;
Paraffin powder 49%.
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 11
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 46%;
Jelly powder 27%;
Paraffin powder 27%.
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Embodiment 12
Pathology embedding medium of the present invention, is comprised of the component of following weight percent:
Pre-gelatinized starch 30%
Jelly powder 35%
Paraffin powder 35%.
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
Adopt pathology embedding medium of the present invention to carry out clinical trial:
Clinical trial one:
Subjects:
Liu: female; Age: 55 years old;
Inspection item: examination (TCT) before cervical cancer
Check hospital: Zhongshan city port hospital
Agents useful for same:
Pathology embedding medium of the present invention.
Simultaneous test:
1, draw materials for the first time, sampling put centrifugal in centrifuge tube after, the supernatant liquor that inclines, gets specimen preparation, dyeing in pipe, microscopy, cell concentration is few, cannot draw a conclusion or not note abnormalities cell.
2, draw materials for the second time, that sampling is put is centrifugal in centrifuge tube, film-making, dyeing, microscopy, and cell concentration is few, cannot draw a conclusion or not note abnormalities cell.
3, draw materials for the third time, centrifugal, the supernatant liquor that inclines in centrifuge tube is put in sampling, with half spoonful of pathology embedding medium of the present invention, inserts in centrifuge tube, and cotton swab stirs, and takes out sample, carries out paraffin embedding, freezing rear section, film-making dyeing, microscopy, conclusion CINI level.
Clinical trial two:
Subjects:
Wang, female; Age: 45 years old;
Inspection item: examination (TCT) before cervical cancer
Check hospital: the Chen Xing of Zhongshan city sea hospital
Agents useful for same: pathology embedding medium of the present invention.
Simultaneous test:
1, draw materials, sampling put centrifugal in centrifuge tube after, the supernatant liquor that inclines, gets specimen preparation dyeing in pipe, microscopy, cell concentration is few, difficulty is drawn a conclusion.
2, with 1/3 spoonful of pathology embedding medium of the present invention, put in centrifuge tube, cotton swab stirs, and takes out sample, carries out paraffin embedding, freezing rear section, film-making dyeing, microscopy, conclusion HSIL.
Pathology embedding medium of the present invention not only can be used in the catching of cell specimen, paraffin embedding film-making, also can be used on the catching of pathology tissue sample in small, broken bits, paraffin embedding film-making.
Claims (5)
1. a pathology embedding medium, is characterized in that being comprised of the component of following weight percent:
Pre-gelatinized starch 1%-90%
Jelly powder 5%-50%
Paraffin powder 5%-50%;
The condition of wherein selecting pre-gelatinized starch is 2g pre-gelatinized starch to be put into 10ml distilled water mix, and after standing 15-20 minute, observes the water surface as clear as crystal, and there is precipitation at the bottom.
2. a method of preparing pathology embedding medium claimed in claim 1, is characterized in that comprising the following steps:
By the weight percent in formula, take each component, then put into clean container and mix encapsulation thoroughly and can obtain pathology embedding medium.
3. the application of a kind of pathology embedding medium claimed in claim 1 on cell specimen is caught.
4. the application that a kind of pathology embedding medium claimed in claim 1 is caught at pathology tissue sample in small, broken bits.
5. a kind of pathology embedding medium claimed in claim 1 is in the application of making in paraffin embedding film-making.
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| CN201110440014.1A CN102532610B (en) | 2011-12-23 | 2011-12-23 | Pathological embedding agent, preparation method and applications thereof |
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| CN201110440014.1A CN102532610B (en) | 2011-12-23 | 2011-12-23 | Pathological embedding agent, preparation method and applications thereof |
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| CN102532610A CN102532610A (en) | 2012-07-04 |
| CN102532610B true CN102532610B (en) | 2014-03-12 |
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| CN106501057A (en) * | 2016-03-29 | 2017-03-15 | 吴菡 | A kind of paraffin-embedded improving technology of cell specimen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101248883A (en) * | 2008-04-01 | 2008-08-27 | 吉林大学 | Egg white high F values oligopeptide buccal tablets and method of preparing the same |
| CN101326937A (en) * | 2008-08-04 | 2008-12-24 | 天津科技大学 | Method for preparing concentrated cheese powder by accelerated fermentation |
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| US6645988B2 (en) * | 1996-01-04 | 2003-11-11 | Curators Of The University Of Missouri | Substituted benzimidazole dosage forms and method of using same |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101248883A (en) * | 2008-04-01 | 2008-08-27 | 吉林大学 | Egg white high F values oligopeptide buccal tablets and method of preparing the same |
| CN101326937A (en) * | 2008-08-04 | 2008-12-24 | 天津科技大学 | Method for preparing concentrated cheese powder by accelerated fermentation |
Non-Patent Citations (2)
| Title |
|---|
| 孙梅等.酪酸菌的微胶囊化研究.《畜牧与饲料科学》.2011,第32卷(第4期),第13-16页. |
| 酪酸菌的微胶囊化研究;孙梅等;《畜牧与饲料科学》;20110430;第32卷(第4期);第13-16页 * |
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Address after: Room 106, building e, junxianju, 328 Changjiang North Road, East District, Zhongshan City, Guangdong Province, 528400 Patentee after: Guangdong Kangnaixin Biomedical Technology Co.,Ltd. Address before: Room 106, building e, junxianju, 328 Changjiang North Road, East District, Zhongshan City, Guangdong Province, 528400 Patentee before: KANG NAI XIN ZHONGSHAN BIOTECHNOLOGY Co.,Ltd. |
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Address after: 528437 whole floor, 2nd floor, No.1 Guangji West Road, Torch Development Zone, Zhongshan City, Guangdong Province Patentee after: Guangdong Kangnaixin Biomedical Technology Co.,Ltd. Address before: Room 106, building e, junxianju, 328 Changjiang North Road, East District, Zhongshan City, Guangdong Province, 528400 Patentee before: Guangdong Kangnaixin Biomedical Technology Co.,Ltd. |