CN103266163A - Combined detection kit for detecting and identifying candida and trichomonias - Google Patents
Combined detection kit for detecting and identifying candida and trichomonias Download PDFInfo
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Abstract
The invention discloses a combined detection kit for detecting and identifying candida and trichomonias. The kit comprises a coagulase activity detection reagent, an alpha-glucosidase activity detection reagent and a pH detection reagent; the enzyme substrate used by the coagulase activity detection reagent is a Gly-Arg dipeptide substrate containing chromogen or fluorophore; and the enzyme substrate used by the alpha-glucosidase activity detection reagent is an alpha-D-glucosidase substrate containing chromogen or fluorophore. In order to improve the color development effect, the kit can also comprise a color development solution. The kit has the advantages that the sensitivity and the specificity are relatively high, the performance is improved remarkably compared with the reagent of the conventional similar method, and the kit can meet the requirement of clinical detection.
Description
Technical field
The present invention relates to technical field of biological, especially relate to the joint inspection test kit of a kind of detection and identification trichomonad and candidiasis.
Background technology
Candidiasis (Candida) is modal conditioned pathogen in fungi, claims again candiyeast, is one of main pathogens caused people's urogenital tract disease, mainly comprises Candida albicans, Candida glabrata, Oidium tropicale and gram Rou Shi candidiasis etc.Under normal circumstances, the coccus of reading colonized in human body is the yeast cell type, generally not pathogenic.Under some physiology of body, pathological factor impact, environment change in vagina/penis, when Abwehrkraft des Koepers/immunizing power reduces, candidiasis will be developed into the mycelia type by amount reproduction, invades tissue, causes moniliosis, so candidiasis is a kind of conditioned pathogen.These factors generally include gestation, diabetes, oral contraceptive, microbiotic and cortin use etc.Woman vagina epithelial cell glycogen increases, and during acid the enhancing, candidiasis can breed and cause vaginitis, therefore this disease is common in pregnant woman, diabetic subject and accepts the patient of estrin treatment.The laboratory examination method has at present: l, smear for microscopic examination: directly microscopy is very simple laboratory method.The women gets oyster white film on vagina, cervical secretions or vaginal wall with longer aseptic cotton swab, and male sex's scraping glans penis, coronary sulcus or foreskin skin damage the surperficial scales of skin that peel off as sample to be checked.Get a little sample secretory product to be checked and be placed on sheet glass, add 10% potassium hydroxide or physiological saline, slide in covering, be placed under microscope, can see spore and the mycelia of oval candidiasis in groups.As while finding more mycelia, illustrate that candidiasis is in causing a disease the stage, more meaningful to diagnosis.But this method is subject to the impact of experience large, and be subject to the visual field, disturb particle particularly this lubricated added paraffin oil of label taking affect larger.2, culture method: this method susceptibility is very high, but go out, required time is longer as a result, poor in timeliness, possible delay treatment.3, latex agglutination slide test: react with the yeast mannosans of polyclonal antibody and some candidiasis, but have no, can be used in clinical high-quality product.4, biochemical enzyme process: the specific enzymes contained by the detection candidiasis is diagnosed, and this method accuracy is higher, convenient and swift, and visual result is developing perfect at present.
Trichomonad (Trichomonias) is a kind of small protozoon biology amphitrichous, with the naked eye can't see, must examine under a microscope.Have three kinds to be mankind parasites: 1, Trichomonas vaginalis, colonize in vagina, urethra and prostate gland, mainly cause trichomonal vaginitis; 2, Trichomonas hominis, parasitize enteron aisle, causes enteron aisle trichomoniasis; 3, buccalis, parasitize oral cavity, tooth dirt, causes oral cavity trichomoniasis.The laboratory examination method has at present: 1, sessile drop method: a corpse or other object for laboratory examination and chemical testing is coated on slide glass, then adds cover glass after adding 1 physiological saline, micro-Microscopic observation, visible protozoon flagellum undulating membrane activity.Sessile drop method is to check the simple method of Trichomonas vaginalis, but has the low problem of sensitivity, because it is by observing the mobility of trichomonad that this method detects trichomonad, and the mobility temperature influence of trichomonad is larger, and when temperature is hanged down, mobility obviously reduces.2, smear staining method: secretory product is coated on slide, available different dye liquors dyeing after seasoning, as gram's staining etc., but this method is subject to size and the similar disturbance caused of trichomonad, and affected by field range.3, culture method: vaginal secretions or urethral secretions are added in substratum, put in 37 ℃ of incubators and cultivate 48 hours, every inoculation in 72 hours 1 time, get and cultivate 1 smear of mixing liquid, dyeing microscopic examination, this method detection required time is long, and clinical general needs went out result in one hour, affected clinical practice and used.4. immunological method: detect the specific antigen of Trichomonas vaginalis.Immunological method commonly used has the fluorescence antibody test procedure, the ELISA method, and latex agglutination etc., its positive rate is high than smear method, but be actually used in the high-quality immunology product that clinical trichomonad detects, has no report.
The disease that candidiasis and trichomonad cause directly is difficult to differentiate from symptom, often needs to be aided with the laboratory detection means.And the method that can directly differentiate at present trichomonad and candidiasis has sediments microscope inspection and immunologic method.Sediments microscope inspection is subject to the impact of other particles in reviewer's experience, field of view and sample large; Immunologic method is limit by technology, and particularly pathogenic agent is affected by the leukorrhea parcel, and the sensitivity meeting is affected.The zymochemistry detection technique that the specific enzymes that utilizes Institute of Micro-biology to have detects corresponding microorganism is the another large technique direction of microorganism detection, in fact increasing scientific and technical personnel are just attempting utilizing this technology for detection trichomonad and candidiasis, such as by cystyl proteolytic enzyme, detecting trichomonad, detect candidiasis by amino-succinamic acid proteolytic enzyme, but its sensitivity and specificity all remain to be investigated.The method that can detect simultaneously and differentiate at present trichomonad and candidiasis has N-acetyl-hexosaminidase method, but this method sensitivity and specificity are all on the low side.
Summary of the invention
The object of the present invention is to provide a kind of highly sensitive and specificity good and can meet the joint inspection test kit of detection and identification trichomonad and the candidiasis of clinical detection needs.
For achieving the above object, the present invention can take following technical proposals:
The joint inspection test kit of detection and identification trichomonad of the present invention and candidiasis, it comprises the active detection reagent of Thrombin coagulase, alpha-glucosidase activity detection reagent and pH detection reagent; The active detection reagent of described Thrombin coagulase enzyme substrates used is Gly-Arg bis-peptide substrates that contain chromogen or fluorophor; Described alpha-glucosidase activity detection reagent enzyme substrates used is the alpha-D-glucose glycosides substrate that contains chromogen or fluorophor.
For improving color developing effect, it also comprises nitrite ion; The solution that nitrite ion is the preparations such as diazenium compound or oxygenant, comprise phenylacrolein, solid purple B salt, solid blue B salt, solid blue BB salt, fast red, urea peroxide, iron trichloride etc.
Chromogen in described Gly-Arg bis-peptide substrates is 4-methoxyl group-beta-naphthylamine, beta-naphthylamine or N-methyl-p-nitroaniline; Fluorophor in described Gly-Arg bis-peptide substrates is coumarin derivatives or Umbelliferone derivative.
Chromogen in described alpha-D-glucose glycosides substrate is Indophenols, nitro benzene and its derivative, naphthol derivative or anils
;fluorophor in described alpha-D-glucose glycosides substrate is Umbelliferone derivative or coumarin derivatives
.
The active detection reagent of described Thrombin coagulase and alpha-glucosidase activity detection reagent can be solidificated on the solid phase carrier of rag paper, filter paper, glass fibre or plastics, also can directly be mixed with liquid and use.
Described pH detection reagent can be solidificated on the solid phase carrier of rag paper, filter paper, glass fibre or plastics, also can directly be mixed with liquid and use.
The using method of test kit of the present invention is:
By sample to be detected (as vaginal secretions) with after appropriate normal saline dilution, getting respectively one (25-50 μ l) is added on the active Test paper of Thrombin coagulase, alpha-glucosidase activity Test paper and pH Test paper, the pH Test paper can directly be read the pH value, together with the active Test paper of alpha-glucosidase activity Test paper and Thrombin coagulase 37 ℃ of lower incubations 15 minutes, after taking-up, according to Show Color, read result or according to the fluorescence intensity reading result.Wherein the active Test paper of Thrombin coagulase and alpha-glucosidase activity Test paper also can drip nitrite ion promotion colour developing.
The principle of the invention is:
Thrombin coagulase (Coagulase) refers to and can make the people of containing Sodium Citrate or anticoagulant heparin agent or the enzyme material that rabbit plasma solidifies, and impels after blood coagulation the effect that can protect pathogenic bacteria not engulfed or avoid antibody etc.Mainly result from staphylococcus, yersinia pestis, candidiasis etc.Alpha-glucosidase (α-Glucosidase, EC 3.2.1.20) be otherwise known as alpha-glucosaccharase lytic enzyme or glucanotransferase (GTase) are a kind of alpha-D-glucose glycosides enzymes.It can cut α-Isosorbide-5-Nitrae glycosidic link and discharge glucose from the non-reduced end of oligosaccharides substrate, the glucosyl residue that maybe will dissociate is transferred to another carbohydrate substrate formation α-1,6 glycosidic link, thereby obtains non-fermentable oligose.The alpha-glucosidase wide material sources, have important physiological function aspect the sugar metabolism of the degraded of human body glycogen and animals and plants, microorganism.
Do not carry out clinically at present the Thrombin coagulase test item, alpha-glucosidase is generally used for the detection of II type glycogen storage disease and male infertility clinically.Find after deliberation, trichomonad has alpha-glucosidase and Thrombin coagulase activity, Candida albicans, Candida glabrata and Oidium tropicale have too this enzyme and live, although gram Rou Shi candidiasis etc. are without this enzymic activity, but Candida albicans, Candida glabrata and Oidium tropicale account in all cause of disease candidiasis more than 95%, therefore with alpha-glucosidase and Thrombin coagulase activity, can detect trichomonad and most candidiasis.Trichomonad belongs to anaerobe in addition, and its metabolic process produces a large amount of rudimentary organic amines, thereby the pH value of sample is obviously raise, and reaches 5.0 even higher, and the sample that infects candidiasis generally detect can be lower than 4.5.Experiment shows, the sample pH value that infects trichomonad all is not less than 4.8, and the pH value of sample that infects candidiasis is higher than 4.6, thereby can distinguish trichomonad and candidiasis to come by the pH value.Therefore by alpha-glucosidase, Thrombin coagulase and pH value, can come trichomonad and candidiasis detection and identification, be alpha-glucosidase and Thrombin coagulase complete negative or any one negatively show not infect trichomonad or candidiasis, alpha-glucosidase and the Thrombin coagulase positive simultaneously show to infect trichomonad or candidiasis, if wherein simultaneously pH value is not less than 4.8 and shows to infect trichomonad, if while pH value does not show to infect candidiasis higher than 4.6.
The invention has the advantages that sensitivity and specificity are all higher, significantly improve than the existing performance with class methods reagent, can meet the clinical detection needs.
The routine Zhengzhou City of clinical trial 2523 healthcare hospital for women & children outpatients of gynecology, pass through vaginal dilator, adopt four parts of posterior fornix of vagina secretory product with aseptic examination, a employing sessile drop method microscopy trichomonad and candidiasis, a employing test kit of the present invention detects trichomonad and candidiasis, another two parts adopt respectively Sha Baoluo substratum and trichomonad culture medium culturing to detect candidiasis and trichomonad, take culture method as gold standard.Detected result sees the following form.
Table 1 test kit of the present invention and culture method trichomonad detect comparing result
Table 2 test kit of the present invention and culture method candidiasis detect comparing result
The above results demonstration, the sensitivity that adopts test kit detection and identification trichomonad of the present invention is 97.5%, specificity is 98.0%; The sensitivity of detection and identification candidiasis is 85.5%, and specificity is 89.6%.
The using method of test kit of the present invention is convenient and swift, does not need the specific installations such as microscope (needing luminoscope or ultraviolet lamp while adopting fluorogenic substrate), in the situation that heating can go out result in 15 minutes; Detected result is objective reliable, owing to being according to the colour-change judged result, has greatly reduced the impact of testing staff's subjective factor; Simple to operate, without long experience accumulation, be easy to apply.
Embodiment
Embodiment 1
One, the preparation of test kit
1, claim glycyl-arginine-4-methoxyl group-beta-naphthylamine hydrochloride (Gly-Arg-4-methoxy-β-naphthylamineHCl, Sigma-Aldrich company produces) (also available glycyl-arginine-beta-naphthylamine), dissolve with 400mM phosphate buffered saline buffer (pH4.0-8.0), make its concentration reach 2-5mg/ml; Title is dissolved in 1000ml 0.3mol/L H to dimethylamino phenylacrolein (can be also paradimethy laminobenzaldehyde) 0.2g
2sO
4in solution; Above two kinds of liquor capacities, than 1:1, mix, and by the 10ul/ sheet, are added on the round scraps of paper of 5mm diameter, and dried overnight, obtain the Thrombin coagulase Test paper.
2, claim chloro-3 indoles of 6--a-D-glucopyranoside (Ka Bosensi chemistry science and technology (Suzhou) company limited produces) (also can use chloro-3 indoles of 5-Br-6--a-D-glucopyranoside, the chloro-3-indoles of 5-Br-4--a-D-glucopyranoside), with after appropriate dissolve with methanol, be dissolved in again 400mM phosphate buffered saline buffer (pH5.0), make its concentration reach 0.5-2mg/ml, mix.By the 10ul/ sheet, be added on the round scraps of paper of 5mm diameter, dried overnight, obtain a-glucuroide Test paper.
3, take the tetrabromo-mcresolsulfonphthalein anhydrous ethanol solvent, after mixing, make its concentration reach 2mg/ml, be added on the scraps of paper of length of side 5mm by 15 μ l/ sheets, dried overnight, obtain the pH value test paper.
4, claim solid purple B salt to dissolve by purified water, mix, make its concentration reach 0.5g/l, can obtain nitrite ion.
Two, test kit using method of the present invention
1, sample to be detected (as vaginal secretions) is diluted with appropriate physiological saline (0.3-0.5ml).
2, get respectively one (25-50 μ l) and be added on the active Test paper of Thrombin coagulase, alpha-glucosidase activity Test paper and pH Test paper, the pH Test paper can directly be read the pH value.
3, on the alpha-glucosidase activity Test paper, add after a nitrite ion together with the active Test paper of Thrombin coagulase 37 ℃ of lower incubations 15 minutes, after taking-up, according to Show Color, reading result: alpha-glucosidase Test paper and Thrombin coagulase Test paper show red positive result, can be divided into again weak positive findings (being labeled as " ± ") and strong positive result (being labeled as "+") according to the colour developing depth, do not develop the color or show the negative result of shallow faint colour (being labeled as "-").
4, interpretation as a result
Embodiment 2
One, test kit preparation
1, claim Z-glycyl-arginine-p-Nitroaniline (Z-Gly-L-Arg-4-nitroanilide, Shanghai vast great chemical industry company limited produces), dissolve with 400mM phosphate buffered saline buffer (pH4.0), make its concentration reach 5mg/ml; By the 10ul/ sheet, be added on the round scraps of paper of 5mm diameter, dried overnight, obtain the Thrombin coagulase Test paper.
2, claim 6-Br-2-naphthols-a-D-glucopyranoside (Ka Bosensi chemistry science and technology (Suzhou) company limited produces) (also can use 6-Br-1-naphthols-a-D-glucopyranoside), with after appropriate dissolve with ethanol, be dissolved in again 400mM phosphate buffered saline buffer (pH5.5), make its concentration reach 2mg/ml, mix.By the 10ul/ sheet, be added on the round scraps of paper of 5mm diameter, dried overnight, obtain the alpha-glucosidase Test paper.
3, take the tetrabromo-mcresolsulfonphthalein anhydrous ethanol solvent, after mixing, make its concentration reach 2mg/ml, be added on the scraps of paper of length of side 5mm by 15 μ l/ sheets, dried overnight, obtain the pH value test paper.
4, claim solid purple B salt to dissolve by purified water, mix, make its concentration reach 0.5g/l, can obtain nitrite ion.
Two, test kit using method of the present invention
1, sample to be detected (as vaginal secretions) is diluted with appropriate physiological saline (0.3-0.5ml).
2, get respectively one (25-50 μ l) and be added on the active Test paper of Thrombin coagulase, alpha-glucosidase activity Test paper and pH Test paper, the pH Test paper can directly be read the pH value.
3, on the alpha-glucosidase activity Test paper, add after a nitrite ion together with the active Test paper of Thrombin coagulase 37 ℃ of lower incubations 15 minutes, after taking-up, according to Show Color, reading result: the alpha-glucosidase Test paper shows red positive result, can be divided into again weak positive findings (being labeled as " ± ") and strong positive result (being labeled as "+") according to the colour developing depth, do not develop the color or show the negative result of shallow faint colour (being labeled as "-").The Thrombin coagulase Test paper shows yellow positive result, according to the colour developing depth, can be divided into again weak positive findings (being labeled as " ± ") and strong positive result (being labeled as "+"), does not develop the color or shows the negative result of shallow faint colour (being labeled as "-").
4, interpretation as a result
Embodiment 3
One, test kit preparation of the present invention
1, claim glycyl-arginine-7-amino-4-methylcoumarin (Gly-L-Arg-7-amino-4-methylcoumarin, gill biochemical company limited in Shanghai produces), dissolve with 100-400mM Tris-HCl damping fluid (pH8.0), make its concentration reach 0.2-1mg/ml; By the 10ul/ sheet, be added on the round scraps of paper of 5mm diameter, dried overnight, obtain the Thrombin coagulase Test paper.
2, claim 4-methyl umbelliferone-α-D-glucopyranoside (Ka Bosensi chemistry science and technology (Suzhou) company limited produces) (also can use Vanillin-α-D-glucopyranoside, 4-methylcoumarin-α-D-glucopyranoside, 4-methylcoumarin-2-deoxidation-2-sulfuric acid amino-α-D-glucopyranoside sodium), with after appropriate dmso solution, be dissolved in again 100mM-400mM acetate buffer (pH5.1), make its concentration reach 1mg/ml, mix.By the 10ul/ sheet, be added on the round scraps of paper of 5mm diameter, dried overnight, obtain a-glucuroide Test paper.
3, take the tetrabromo-mcresolsulfonphthalein anhydrous ethanol solvent, after mixing, make its concentration reach 2mg/ml, be added on the scraps of paper of length of side 5mm by 15 μ l/ sheets, dried overnight, obtain the pH value test paper.
Two, test kit using method of the present invention
1, sample to be detected (as vaginal secretions) is diluted with appropriate physiological saline (0.3-0.5ml).
2, get respectively one (25-50 μ l) and be added on the active Test paper of Thrombin coagulase, alpha-glucosidase activity Test paper and pH Test paper, the pH Test paper can directly be read the pH value.
3, together with the active Test paper of alpha-glucosidase activity Test paper and Thrombin coagulase at 37 ℃ of lower incubations after 15 minutes, observe the fluorescence situation with Perkin Elemer LS50B luminoscope, reading result: alpha-glucosidase Test paper and Thrombin coagulase Test paper can be divided into weak positive findings (being labeled as " ± ") and strong positive result (being labeled as "+") according to fluorescence intensity, do not develop the color or show the negative result of shallow faint colour (being labeled as "-").
4, interpretation as a result
。
Claims (6)
1. the joint inspection test kit of a detection and identification trichomonad and candidiasis, it is characterized in that: it comprises the active detection reagent of Thrombin coagulase, alpha-glucosidase activity detection reagent and pH detection reagent;
The active detection reagent of described Thrombin coagulase enzyme substrates used is Gly-Arg bis-peptide substrates that contain chromogen or fluorophor;
Described alpha-glucosidase activity detection reagent enzyme substrates used is the alpha-D-glucose glycosides substrate that contains chromogen or fluorophor.
2. the joint inspection test kit of detection and identification trichomonad according to claim 1 and candidiasis, it is characterized in that: it also comprises nitrite ion.
3. the joint inspection test kit of detection and identification trichomonad according to claim 1 and 2 and candidiasis, it is characterized in that: the chromogen in described Gly-Arg bis-peptide substrates is 4-methoxyl group-beta-naphthylamine, beta-naphthylamine, N-methyl-p-nitroaniline
;fluorophor in described Gly-Arg bis-peptide substrates is coumarin derivatives or Umbelliferone derivative
.
4. the joint inspection test kit of detection and identification trichomonad according to claim 1 and 2 and candidiasis, it is characterized in that: described alpha-D-glucose glycosides substrate chromogen comprises Indophenols, nitro benzene and its derivative, naphthol derivative or anils; Described alpha-D-glucose glycosides substrate fluorophor comprises Umbelliferone derivative or coumarin derivatives.
5. the joint inspection test kit of detection and identification trichomonad according to claim 1 and 2 and candidiasis, it is characterized in that: the active detection reagent of described Thrombin coagulase and alpha-glucosidase activity detection reagent can be solidificated on the solid phase carrier of rag paper, filter paper, glass fibre or plastics, also can directly be mixed with liquid and use.
6. the joint inspection test kit of detection and identification trichomonad according to claim 1 and candidiasis, it is characterized in that: described pH detection reagent can be solidificated on the solid phase carrier of rag paper, filter paper, glass fibre or plastics, also can directly be mixed with liquid and use.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760331A (en) * | 2014-02-11 | 2014-04-30 | 郑州安图生物工程股份有限公司 | Combined detection kit for seminal plasma |
| CN105463056A (en) * | 2016-01-21 | 2016-04-06 | 中国人民解放军第四军医大学 | Culture medium for rapid culture and developing of candida albicans and detection method |
| CN106645721A (en) * | 2016-09-30 | 2017-05-10 | 广州鸿琪光学仪器科技有限公司 | Coagulase detection reagent, reaction pad, preparation method thereof and kit |
| CN109705177A (en) * | 2018-12-24 | 2019-05-03 | 郑州安图生物工程股份有限公司 | A kind of substrate and its preparation method and application |
| CN109991218A (en) * | 2019-03-29 | 2019-07-09 | 迪瑞医疗科技股份有限公司 | A kind of preparation method of coagulase Test paper |
| CN111349685A (en) * | 2020-02-27 | 2020-06-30 | 浙江省农业科学院 | Rapid screening method and application of α -glycosidase inhibitor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294952A (en) * | 2007-04-23 | 2008-10-29 | 廖秦平 | Novel vaginal micro-ecological appraisement system, reagent kit for appraising vaginal micro-ecology |
| CN101432740A (en) * | 2004-07-27 | 2009-05-13 | 麦德迈恩德有限公司 | Method for measuring the incidence of hospital acquired infections |
| CN102253206A (en) * | 2011-06-23 | 2011-11-23 | 泰普生物科学（中国）有限公司 | Combined detection kit for vaginitis |
-
2013
- 2013-06-13 CN CN201310232673.5A patent/CN103266163B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101432740A (en) * | 2004-07-27 | 2009-05-13 | 麦德迈恩德有限公司 | Method for measuring the incidence of hospital acquired infections |
| CN101294952A (en) * | 2007-04-23 | 2008-10-29 | 廖秦平 | Novel vaginal micro-ecological appraisement system, reagent kit for appraising vaginal micro-ecology |
| CN102253206A (en) * | 2011-06-23 | 2011-11-23 | 泰普生物科学（中国）有限公司 | Combined detection kit for vaginitis |
Non-Patent Citations (1)
| Title |
|---|
| 齐慧敏: "2000~2001年13家医院泌尿系统感染细菌耐药状况调查", 《中华肾脏病杂志》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760331A (en) * | 2014-02-11 | 2014-04-30 | 郑州安图生物工程股份有限公司 | Combined detection kit for seminal plasma |
| CN103760331B (en) * | 2014-02-11 | 2016-05-04 | 郑州安图生物工程股份有限公司 | Refining joint inspection kit |
| CN105463056A (en) * | 2016-01-21 | 2016-04-06 | 中国人民解放军第四军医大学 | Culture medium for rapid culture and developing of candida albicans and detection method |
| CN105463056B (en) * | 2016-01-21 | 2019-02-22 | 中国人民解放军第四军医大学 | Candida albicans fast culture and the culture medium to develop the color and detection method |
| CN106645721A (en) * | 2016-09-30 | 2017-05-10 | 广州鸿琪光学仪器科技有限公司 | Coagulase detection reagent, reaction pad, preparation method thereof and kit |
| CN106645721B (en) * | 2016-09-30 | 2018-10-09 | 广州鸿琪光学仪器科技有限公司 | A kind of coagulase detection reagent, reacting pad, preparation method and kit |
| CN109705177A (en) * | 2018-12-24 | 2019-05-03 | 郑州安图生物工程股份有限公司 | A kind of substrate and its preparation method and application |
| CN109991218A (en) * | 2019-03-29 | 2019-07-09 | 迪瑞医疗科技股份有限公司 | A kind of preparation method of coagulase Test paper |
| CN111349685A (en) * | 2020-02-27 | 2020-06-30 | 浙江省农业科学院 | Rapid screening method and application of α -glycosidase inhibitor |
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