CN108552162A - A kind of urine preserves liquid, preparation method and its pelletizer - Google Patents
A kind of urine preserves liquid, preparation method and its pelletizer Download PDFInfo
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- CN108552162A CN108552162A CN201810623997.4A CN201810623997A CN108552162A CN 108552162 A CN108552162 A CN 108552162A CN 201810623997 A CN201810623997 A CN 201810623997A CN 108552162 A CN108552162 A CN 108552162A
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- urine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/0231—Chemically defined matrices, e.g. alginate gels, for immobilising, holding or storing cells, tissue or organs for preservation purposes; Chemically altering or fixing cells, tissue or organs, e.g. by cross-linking, for preservation purposes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Wood Science & Technology (AREA)
- Dispersion Chemistry (AREA)
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Abstract
Preserve liquid, preparation method and its pelletizer the present invention relates to a kind of urine, the urine preserve liquid include following components by mass percentage:Sodium citrate 2%~4%, sodium chloride 0.08%~0.12%, potassium chloride 0.03%~0.05%, calcium chloride 0.02%~0.03%, formaldehyde 20%~30%, ethyl alcohol 30%~40%, glacial acetic acid 1%~2%, surplus are distilled water;The preparation method of liquid is preserved according to urine described in claim;The pelletizer of the urine test sample includes flat-ended tube, pipe lid and coverslip;It is of low cost that urine of the present invention preserves liquid, preparation method is simple, convenient for promoting the use of, can quickly macrocyclic fixed preservation urine cell, urine cellular morphology remain intact, meet requirement of the urine test to effective cell quality and quantity, the preparation method that urine preserves liquid is simple, and three-waste free pollution generates, the pelletizer of urine test sample, it is simple in structure, it is easy to use.
Description
Technical field
The present invention relates to medicine technology fields, and in particular to a kind of urine preserves liquid, preparation method and its pelletizer.
Background technology
Urine is the excreta being of great significance, the variation reflection urinary systems of urinary fractions and its hetero-organization organ
The accuracy of lesion, testing result is directly related to the Clinics and Practices of disease.Urine cytology inspection is for finding to secrete
A kind of pathological examination method of urinary system malignant tumour.
Urine is a kind of unstable substance, will occur certain chemical changes once discharge, store 6h, 12h and for 24 hours after
Inspection result and sample when rigid discharge it is different, main reasons is that the corruption of urine sample, tangible or nothing occurs for when urine corruption
The variation of shape, pH value may increase or reduce, color burn, smell is rancid, turbidity increases, bilirubin is reduced or disappears, ketone
Body is reduced or is disappeared, glucose in urine is reduced or disappears, urobilinogen is reduced or disappear, bacterium increases, mould increases, cast reduces or disappears
It loses, red blood cell dissolves and deformation, occult blood enhancing, albumen can increase or decrease, nitrite false positive, ammonia increase, vitamin subtracts
It less or disappears, this series of variation all damages urine cellular morphology.Urine currently on the market preserves liquid, in fixation
Urine cell keeps eucaryotic cell structure stability bad, and the retention time is not grown, and more than three days cells are perishable at room temperature, and
It all uses 40% methanol addition formaldehyde as fixed preservative, easily causes the agglomerating caking of cell, also easily caking is heavy for protein mucus
It forms sediment, is unfavorable for the dispersion and observation of cell.
When urine detection, after first acquiring freshly voided urine, urine is added and preserves liquid, tumour and non-tumor cell is made to keep
Initial cell form when acquisition, and so that cell is uniformly coated on glass slide by film-making and thin-layer cell smear is made, it dyes
Eucaryotic cell structure is clearly easily debated under the microscope afterwards.
Invention content
The purpose of the present invention is to provide a kind of urines to preserve liquid, preparation method and its pelletizer, existing to solve
Urine preserves liquid and there is the problems such as fixed urine cell, keep urine eucaryotic cell structure stability poor.
To achieve the above object, the technical scheme is that:
A kind of urine preserves liquid, including following components is by mass percentage:Sodium citrate 2%~4%, sodium chloride
0.08%~0.12%, potassium chloride 0.03%~0.05%, calcium chloride 0.02%~0.03%, formaldehyde 20%~30%, ethyl alcohol
30%~40%, glacial acetic acid 1%~2%, surplus are distilled water;It is the key component for fixing urine cell with formaldehyde and ethyl alcohol,
It can quickly macrocyclic fixed preservation urine cell, urine cellular morphology remain intact, meet urine test to effective cell
The requirement of quality and quantity, while formaldehyde can kill the microbial impurities in urine, have bacteriostasis, it can be ensured that urine sample
Product do not go mouldy during preserving, and do not influence film-making observation as a result, ethyl alcohol has fixed dehydrating agent and disinfectant effect, and substantially
Human body will not be damaged, the methanol that urine preservation liquid contains on the market is solved at present and testing staff's actual bodily harm is asked
Topic, sodium chloride, potassium chloride and calcium chloride are used as buffer solution, keep urine Premeabilisation of cells pressure stabilization, will not expand, pyknosis etc.
Metamorphosis, prevents urine cell rupture, and sodium citrate is added as stabilizer, prevents the epithelial cell to fall off in urine and in vain
Cell degradation is agglomerated, and urine sample detection is influenced, and glacial acetic acid is used for adjusting the pH value that urine preserves liquid, ensures that the biology of urine is living
Property;
Further, it includes that following components is by mass percentage that the urine, which preserves liquid,:Sodium citrate 2.4%, chlorine
Change sodium 0.10%, potassium chloride 0.04%, calcium chloride 0.02%, formaldehyde 26%, ethyl alcohol 36%, glacial acetic acid 1.5%, distilled water
33.94%;
Further, the pH value that the urine preserves liquid is 6.5~7.4;
Further, the mass concentration of the ethyl alcohol is 95%~100%, the mass concentration of the formaldehyde is 35%~
45%;
A kind of urine preserves the preparation method of liquid, includes the following steps:
A. sodium chloride, potassium chloride and calcium chloride are weighed by formula ratio, is put into mixer and is uniformly mixed;
B. the distilled water of formula ratio, stirring and dissolving are added into mixture obtained by step A;
C. the formaldehyde and ethyl alcohol of formula ratio are added into step B acquired solutions, is stirred to obtain mixed liquor;
D. the sodium citrate of formula ratio, stirring and dissolving mixing are added in the mixed liquor obtained by step C;
E. the glacial acetic acid stirring and dissolving of formula ratio is added into step D acquired solutions, adjusts pH value.
It is a kind of to preserve the pelletizer that liquid prepares urine test sample, including flat-ended tube, pipe lid and coverslip using urine,
The coverslip is lain in a horizontal plane in flat-ended tube, and the pipe lid is connected with the upper, open end of flat-ended tube by screw thread, is conveniently taken
Put urine sample;
Further, the material of the flat-ended tube is transparent glass or plastics, facilitates color, the turbidity of observation urine sample
Material Deng, the pipe lid is plastics;
Further, the volume of the flat-ended tube is 50ml, a diameter of 30mm, a diameter of 28mm of the coverslip, lid
Slide can be conveniently placed in flat-ended tube;
Further, it is equipped with scale on the outside of the tube wall of the flat-ended tube, conveniently measures the volume of urine sample.
The thin-layer cell smear that urine sample is made using above-mentioned pelletizer, is included the following steps:
A. the pipe lid is opened, urine is packed into flat-ended tube and preserves liquid and the mixed urine sample of freshly voided urine, lid
The good pipe lid;
B. the flat-ended tube is put into centrifuge, is centrifuged 5 minutes under the acceleration of gravity of 2000G;
C. the coverslip in the flat-ended tube is taken out, progress urine cytology test experience in flat ware is put into.
The invention has the advantages that:
1, urine of the present invention preserves the enough quickly macrocyclic fixed preservation urine cells of liquid energy, and urine cellular morphology has been kept
It is good, meet requirement of the urine test to effective cell quality and quantity, while formaldehyde can kill the microbial impurities in urine,
With bacteriostasis, it can be ensured that urine sample does not go mouldy during preserving, and does not influence film-making observation as a result, ethyl alcohol has fixation
Dehydrating agent and disinfectant effect, and human body will not be damaged substantially, it solves at present urine on the market and preserves what liquid contained
The problem of methanol is to testing staff's actual bodily harm, sodium chloride, potassium chloride and calcium chloride keep urine Premeabilisation of cells as buffer solution
The stability of pressure will not expand, the metamorphosis such as pyknosis, prevent urine cell rupture, and sodium citrate is added as stablizing
Agent prevents the epithelial cell to fall off in urine and leucocyte from degrading and agglomerates, and influences urine sample detection, and glacial acetic acid is used for adjusting urine
Liquid preserves the pH value of liquid, ensures the bioactivity of urine.
2, raw material used in urine preservation liquid of the present invention is simple and easy to get, of low cost, convenient for promoting the use of, preparation method
Simply, three-waste free pollution generates, and does not generate the medical waste for needing specially treated in use.
3, the present invention is a kind of preserving the pelletizer that liquid prepares urine test sample, simple in structure, user using urine
Just, flat-ended tube is equipped with scale, conveniently measures the volume of urine sample, and flat-ended tube uses transparent glass or plastic material, just
In color, the turbidity etc. of observation urine sample.
Description of the drawings
Fig. 1 is a kind of to preserve the structural schematic diagram that liquid prepares the pelletizer of urine test sample using urine.
Urine sample testing result figure after being stored 3 days using urine cell-preservation liquid of the present invention in Fig. 2 embodiments 1.
Urine sample testing result figure after being stored 1 week using urine cell-preservation liquid of the present invention in Fig. 3 embodiments 1.
Urine sample testing result figure after being stored 1 month using urine cell-preservation liquid of the present invention in Fig. 4 embodiments 1.
Urine sample testing result figure after being stored 2 months using urine cell-preservation liquid of the present invention in Fig. 5 embodiments 1.
Urine sample testing result figure after being stored 4 months using urine cell-preservation liquid of the present invention in Fig. 6 embodiments 1.
Urine sample testing result figure after being stored 6 months using urine cell-preservation liquid of the present invention in Fig. 7 embodiments 1.
Wherein, 1. Pingdu pipes, 2. pipe lids, 3. coverslips.
Specific implementation mode
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
A kind of urine preservation liquid:
Component is constituted:It is by mass percentage:Sodium citrate 20g, sodium chloride 0.8g, potassium chloride 0.3g, calcium chloride
0.2g, formaldehyde 244ml, ethyl alcohol 380ml, glacial acetic acid 10g, distilled water 469ml.
Preparation method:
A. sodium chloride 0.8g, potassium chloride 0.3g, calcium chloride 0.2g are weighed by formula, is put into mixer and is stirred
It is even;
B. distilled water 469ml, stirring and dissolving is added by formula ratio into mixture obtained by step A;
C. formaldehyde 244ml, ethyl alcohol 380ml is added by formula ratio into step B acquired solutions, is stirred to obtain mixed liquor;
D. sodium citrate 20g, stirring and dissolving mixing is added by formula ratio in the mixed liquor obtained by step C;
E. glacial acetic acid 10g is added into step D acquired solutions by formula ratio, adjusts pH value to 6.5.
As shown in Figure 1, a kind of preserving the pelletizer that liquid prepares urine test sample, including flat-ended tube 1, pipe using urine
Lid 2 and coverslip 3,1 upper end opening of the flat-ended tube, lower end closed, material is transparent glass, and the material of the pipe lid 2 is modeling
Material, the volume of the flat-ended tube 1 are 50ml, a diameter of 30mm, are provided with scale on the outside of tube wall, the coverslip 3 it is a diameter of
28mm is lain in a horizontal plane in flat-ended tube 1, and the pipe lid 2 is connected with the upper, open end of flat-ended tube 1 by screw thread.
The thin-layer cell smear of urine sample is made using the pelletizer of urine test sample, carries out cytology inspection:
Freshly voided urine 20ml is acquired, above-mentioned 20ml urines preservation liquid is added and is uniformly mixed, is divided in 10 pelletizers, covers in equal volume
The good pipe lid 2, marks.Pick and place respectively set 3 days, 1 week, 1 month, 2 months, 4 months and the urine sample after 6 months into
The flat-ended tube 1 is put into centrifuge by row film-making, is centrifuged 5 minutes under the acceleration of gravity of 2000G, described in taking-up
Coverslip 3 is put into flat ware, observes urine cellular morphology, turbidity etc. under the microscope, and acquires image retention.
Experimental result:The urine cell picture that the acquisition of each period is retained is compared, sees attached drawing 2~7:
Serial number | Time | Form | Turbidity | Dyeing |
1 | 3 days | Completely | It is limpid | Basket, Huang |
2 | 1 week | Completely | It is limpid | Basket, Huang |
3 | 1 month | Completely | It is limpid | Basket, Huang |
4 | 2 months | Completely | It is limpid | Basket, Huang |
5 | 4 months | Completely | It is limpid | Basket, Huang |
6 | 6 months | Completely | It is limpid | Basket, Huang |
Picture is detected according to the urine sample that each period preserves, obtains and preserves the urine that liquid preserves using urine of the present invention
Cell keeps form stable, dyeing clear within each period.
Embodiment 2
A kind of urine preservation liquid:
Component is constituted:It is by mass percentage:Sodium citrate 12g, sodium chloride 0.5g, potassium chloride 0.2g, calcium chloride
0.1g, formaldehyde 158ml, ethyl alcohol 228ml, glacial acetic acid 7.5g, distilled water 339ml.
Preparation method:
A. sodium chloride 0.5g, potassium chloride 0.2g, calcium chloride 0.1g are weighed by formula, is put into mixer and is stirred
It is even;
B. the distilled water 339ml of formula ratio, stirring and dissolving are added into mixture obtained by step A;
C. formaldehyde 158ml, ethyl alcohol 228ml is added by formula ratio into step B acquired solutions, is stirred to obtain mixed liquor;
D. sodium citrate 12g, stirring and dissolving mixing is added by formula ratio in the mixed liquor obtained by step C;
E. glacial acetic acid 7.5g is added into step D acquired solutions by formula ratio and adjusts pH value to 7.1.
As shown in Figure 1, a kind of preserving the pelletizer that liquid prepares urine test sample, including flat-ended tube using above-mentioned urine
1, pipe lid 2 and coverslip 3,1 upper end opening of the flat-ended tube, lower end closed, material is transparent plastic, the material of the pipe lid 2
Volume for plastics, the flat-ended tube 1 is 50ml, and a diameter of 30mm, tube wall outside is equipped with scale, the diameter of the coverslip 3
It for 28mm, lies in a horizontal plane in flat-ended tube 1, the pipe lid 2 is connected with the upper, open end of flat-ended tube 1 by screw thread.
The thin-layer cell smear of urine sample is made using the pelletizer of urine test sample, carries out cytology inspection:
Freshly voided urine 20ml is acquired, above-mentioned 20ml urines preservation liquid is added and is uniformly mixed, is divided in 10 pelletizers, covers in equal volume
The good pipe lid 2, marks.Pick and place respectively set 3 days, 1 week, 1 month, 2 months, 4 months and the urine sample after 6 months into
The flat-ended tube 1 is put into centrifuge by row film-making, is centrifuged 5 minutes under the acceleration of gravity of 2000G, described in taking-up
Coverslip 3 is put into flat ware, observes urine cellular morphology, turbidity etc. under the microscope, and acquires image retention.
Experimental result:The urine cell picture that the acquisition of each period is retained is compared:
Serial number | Time | Form | Turbidity | Dyeing |
1 | 3 days | Completely | It is limpid | Basket, Huang |
2 | 1 week | Completely | It is limpid | Basket, Huang |
3 | 1 month | Completely | It is limpid | Basket, Huang |
4 | 2 months | Completely | It is limpid | Basket, Huang |
5 | 4 months | Completely | It is limpid | Basket, Huang |
6 | 6 months | Completely | It is limpid | Basket, Huang |
Picture is detected according to the urine sample that each period preserves, obtains and preserves the urine that liquid preserves using urine of the present invention
Cell keeps form stable, dyeing clear within each period.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention belong to the scope of protection of present invention.
Claims (8)
1. a kind of urine preserves liquid, it is characterised in that:It includes that following components is by mass percentage that the urine, which preserves liquid,:Chinese holly
Rafter acid sodium 2%~4%, sodium chloride 0.08%~0.12%, potassium chloride 0.03%~0.05%, calcium chloride 0.02%~
0.03%, formaldehyde 20%~30%, ethyl alcohol 30%~40%, glacial acetic acid 1%~2%, surplus are distilled water.
2. urine according to claim 1 preserves liquid, it is characterised in that:The urine preservation liquid includes following components by matter
Measuring percentages is:Sodium citrate 2.4%, sodium chloride 0.10%, potassium chloride 0.04%, calcium chloride 0.02%, formaldehyde 26%, second
Alcohol 36%, glacial acetic acid 1.5%, surplus are distilled water.
3. urine according to claim 1 preserves liquid, it is characterised in that:The pH value that the urine preserves liquid is 6.5~
7.4。
4. a kind of preparation method preserving liquid according to urine described in claims 1 to 3 any one, includes the following steps:
A. sodium chloride, potassium chloride and calcium chloride are weighed by formula ratio, is put into mixer and is uniformly mixed;
B. the distilled water of formula ratio, stirring and dissolving are added into mixture obtained by step A;
C. the formaldehyde and ethyl alcohol of formula ratio are added into step B acquired solutions, is stirred to obtain mixed liquor;
D. the sodium citrate of formula ratio, stirring and dissolving mixing are added in the mixed liquor obtained by step C;
E. the glacial acetic acid that formula ratio is added into step D acquired solutions adjusts pH value.
5. a kind of preserving the pelletizer that liquid prepares urine test sample using urine described in claims 1 to 3 any one,
It is characterized in that:It is described using urine preserve liquid prepare urine test sample pelletizer include flat-ended tube (1), pipe lid (2) and
Coverslip (3), the coverslip (3) lie in a horizontal plane in flat-ended tube (1), the upper opening of the pipe lid (2) and flat-ended tube (1)
End is connected by screw thread.
6. pelletizer according to claim 5, it is characterised in that:The material of the flat-ended tube (1) be transparent glass or
The material of plastics, the pipe lid (2) is plastics.
7. pelletizer according to claim 5, it is characterised in that:The volume of the flat-ended tube (1) is 50ml, a diameter of
30mm, a diameter of 28mm of the coverslip (3).
8. pelletizer according to claim 5, it is characterised in that:Scale is equipped on the outside of the tube wall of the flat-ended tube (1).
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109757466A (en) * | 2018-10-29 | 2019-05-17 | 中国医学科学院阜外医院 | Urine saves liquid, urine capture container, method and kit |
CN111134109A (en) * | 2019-12-19 | 2020-05-12 | 苏州浚惠生物科技有限公司 | Urine single cell preservation solution and preparation method thereof |
CN111175099A (en) * | 2019-12-09 | 2020-05-19 | 湖北泰康医疗设备有限公司 | Method for extracting and flaking humoral cells for bladder cancer examination |
CN111323290A (en) * | 2020-02-10 | 2020-06-23 | 广州金域医学检验中心有限公司 | Urine sample processing method, storage medium, equipment and system |
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CN106092679A (en) * | 2016-06-05 | 2016-11-09 | 镇江立昌智能装备有限公司 | Liquid basal cell natural subsidence pelletizer |
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CN202385664U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Bottle for preserving liquid basal cell |
CN103583508A (en) * | 2013-11-25 | 2014-02-19 | 张超 | Preparation method of liquid-based cell treating and preserving solution and application thereof |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109757466A (en) * | 2018-10-29 | 2019-05-17 | 中国医学科学院阜外医院 | Urine saves liquid, urine capture container, method and kit |
CN109757466B (en) * | 2018-10-29 | 2022-05-24 | 中国医学科学院阜外医院 | Urine preserving fluid |
CN111175099A (en) * | 2019-12-09 | 2020-05-19 | 湖北泰康医疗设备有限公司 | Method for extracting and flaking humoral cells for bladder cancer examination |
CN111175099B (en) * | 2019-12-09 | 2024-06-25 | 湖北泰康医疗设备有限公司 | Extraction and tabletting method for body fluid cells for detecting bladder cancer |
CN111134109A (en) * | 2019-12-19 | 2020-05-12 | 苏州浚惠生物科技有限公司 | Urine single cell preservation solution and preparation method thereof |
CN111323290A (en) * | 2020-02-10 | 2020-06-23 | 广州金域医学检验中心有限公司 | Urine sample processing method, storage medium, equipment and system |
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Application publication date: 20180921 |