CN208540656U - A kind of urine preservation liquid prepares the pelletizer of urine test sample - Google Patents
A kind of urine preservation liquid prepares the pelletizer of urine test sample Download PDFInfo
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- CN208540656U CN208540656U CN201820932963.9U CN201820932963U CN208540656U CN 208540656 U CN208540656 U CN 208540656U CN 201820932963 U CN201820932963 U CN 201820932963U CN 208540656 U CN208540656 U CN 208540656U
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Abstract
The utility model relates to a kind of urines to save the pelletizer that liquid prepares urine test sample, it includes flat-ended tube (1), pipe lid (2) and coverslip (3) that the urine, which saves liquid and prepares the pelletizer of urine test sample, the coverslip (3) lies in a horizontal plane in flat-ended tube (1), and the pipe lid (2) is connected with the upper, open end of flat-ended tube (1) by screw thread.It is low in cost that the utility model urine saves liquid, preparation method is simple, convenient for promoting the use of, can quickly macrocyclic fixed preservation urine cell, urine cellular morphology remain intact, meet requirement of the urine test to effective cell quality and quantity, the preparation method that urine saves liquid is simple, and three-waste free pollution generates, the pelletizer of urine test sample, structure is simple, easy to use.
Description
Technical field
The utility model relates to medicine technology fields, and in particular to a kind of urine preservation liquid prepares the system of urine test sample
Sheet devices.
Background technique
Urine is the excreta being of great significance, the variation reflection urinary systems of urinary fractions and its hetero-organization organ
The accuracy of lesion, testing result is directly related to the Clinics and Practices of disease.Urine cytology inspection is for finding to secrete
A kind of pathological examination method of urinary system malignant tumour.
Urine is a kind of unstable substance, and certain chemical changes will occur once discharge, store 6h, 12h and for 24 hours after
Inspection result and sample when rigid discharge it is different, main reasons is that the corruption of urine sample, tangible or nothing occurs for when urine corruption
The variation of shape, pH value may increase or reduce, color burn, smell is rancid, turbidity increases, bilirubin is reduced or disappears, ketone
Body is reduced or is disappeared, glucose in urine is reduced or disappears, urobilinogen is reduced or disappear, bacterium increases, mould increases, cast reduces or disappears
It loses, red blood cell dissolves and deformation, occult blood enhancing, albumen can increase or decrease, nitrite false positive, ammonia increase, vitamin subtracts
It less or disappears, this series of variation all damages urine cellular morphology.Urine currently on the market saves liquid, in fixation
Urine cell keeps eucaryotic cell structure stability bad, and the retention time is not grown, and more than three days cells are perishable at room temperature, and
The agglomerating agglomeration of cell is all easily caused, also easily agglomeration is heavy for protein mucus as fixed preservative using 40% methanol addition formaldehyde
It forms sediment, is unfavorable for the dispersion and observation of cell.
When urine detection, after first acquiring freshly voided urine, urine is added and saves liquid, keep tumour and non-tumor cell
Initial cell form when acquisition, and be uniformly coated on cell on glass slide by film-making and thin-layer cell smear is made, it dyes
Eucaryotic cell structure is clearly easily debated under the microscope afterwards.
Utility model content
The purpose of this utility model is to provide a kind of urines to save liquid, preparation method and its pelletizer, to solve
Existing urine saves liquid and there is the problems such as fixed urine cell, keep urine eucaryotic cell structure stability poor.
To achieve the above object, the technical solution of the utility model are as follows:
A kind of urine preservation liquid, including following components is by mass percentage are as follows: sodium citrate 2%~4%, sodium chloride
0.08%~0.12%, potassium chloride 0.03%~0.05%, calcium chloride 0.02%~0.03%, formaldehyde 20%~30%, ethyl alcohol
30%~40%, glacial acetic acid 1%~2%, surplus are distilled water；It take formaldehyde and ethyl alcohol as the main component of fixed urine cell,
It can quickly macrocyclic fixed preservation urine cell, urine cellular morphology remain intact, meet urine test to effective cell
The requirement of quality and quantity, while formaldehyde can kill the microbial impurities in urine, have bacteriostasis, it can be ensured that urine sample
Product do not go mouldy during saving, and do not influence film-making observation as a result, ethyl alcohol has fixed dehydrating agent and disinfectant effect, and substantially
Human body will not be damaged, the methanol that urine saves that liquid contains on the market is solved at present and testing staff's actual bodily harm is asked
Topic, sodium chloride, potassium chloride and calcium chloride are used as buffer, keep urine Premeabilisation of cells pressure stabilization, will not expand, pyknosis etc.
Metamorphosis, prevents urine cell rupture, and sodium citrate is added as stabilizer, prevents the epithelial cell to fall off in urine and white
Cell degradation cohesion influences urine sample detection, and glacial acetic acid is used to adjust the pH value that urine saves liquid, guarantees that the biology of urine is living
Property；
Further, the urine save liquid include following components by mass percentage are as follows: sodium citrate 2.4%, chlorine
Change sodium 0.10%, potassium chloride 0.04%, calcium chloride 0.02%, formaldehyde 26%, ethyl alcohol 36%, glacial acetic acid 1.5%, distilled water
33.94%；
Further, it is 6.5~7.4 that the urine, which saves the pH value of liquid,；
Further, the mass concentration of the ethyl alcohol is 95%~100%, the mass concentration of the formaldehyde is 35%~
45%；
A kind of urine saves the preparation method of liquid, comprising the following steps:
A. sodium chloride, potassium chloride and calcium chloride are weighed by formula ratio, is put into mixer and is uniformly mixed；
B. the distilled water of formula ratio, stirring and dissolving are added into mixture obtained by step A；
C. the formaldehyde and ethyl alcohol of formula ratio are added into step B acquired solution, is stirred to obtain mixed liquor；
D. the sodium citrate of formula ratio, stirring and dissolving mixing are added in the mixed liquor obtained by step C；
E. the glacial acetic acid stirring and dissolving of formula ratio is added into step D acquired solution, adjusts pH value.
It is a kind of to save the pelletizer that liquid prepares urine test sample, including flat-ended tube, pipe lid and coverslip using urine,
The coverslip is lain in a horizontal plane in flat-ended tube, and the pipe lid is connected with the upper, open end of flat-ended tube by screw thread, is conveniently taken
Put urine sample；
Further, the material of the flat-ended tube is transparent glass or plastics, facilitates color, the turbidity of observation urine sample
Material Deng, the pipe lid is plastics；
Further, the volume of the flat-ended tube is 50ml, and diameter 30mm, the diameter of the coverslip is 28mm, lid
What slide can be convenient is placed in flat-ended tube；
Further, it is equipped with scale on the outside of the tube wall of the flat-ended tube, conveniently measures the volume of urine sample.
Use the thin-layer cell smear of above-mentioned pelletizer production urine sample, comprising the following steps:
A. the pipe lid is opened, urine is packed into flat-ended tube and saves liquid and the mixed urine sample of freshly voided urine, lid
The good pipe lid；
B. the flat-ended tube is put into centrifuge, is centrifugated 5 minutes under the acceleration of gravity of 2000G；
C. the coverslip in the flat-ended tube is taken out, progress urine cytology test experience in flat ware is put into.
The utility model has the advantages that
1, the utility model urine saves liquid and can quickly macrocyclic fixed preservation urine cell, urine cellular morphology protect
It holds intact, meets requirement of the urine test to effective cell quality and quantity, while formaldehyde can kill the microorganism in urine
Impurity has bacteriostasis, it can be ensured that urine sample does not go mouldy during saving, and does not influence film-making observation as a result, ethyl alcohol has
There are fixed dehydrating agent and disinfectant effect, and human body will not be damaged substantially, solves at present urine on the market and save liquid
The problem of methanol contained is to testing staff's actual bodily harm, sodium chloride, potassium chloride and calcium chloride keep urine thin as buffer
The stability of born of the same parents' osmotic pressure will not expand, the metamorphosis such as pyknosis, prevent urine cell rupture, and sodium citrate is added and makees
For stabilizer, prevents the epithelial cell to fall off in urine and leucocyte from degrading and agglomerate, influence urine sample detection, glacial acetic acid is used to
The pH value that urine saves liquid is adjusted, guarantees the bioactivity of urine.
2, raw material used in the utility model urine preservation liquid is simple and easy to get, low in cost, convenient for promoting the use of, preparation
Method is simple, and three-waste free pollution generates, and does not generate the medical waste for needing specially treated in use.
3, the utility model is a kind of saves the pelletizer that liquid prepares urine test sample using urine, and structure is simple, makes
With conveniently, flat-ended tube is equipped with scale, conveniently measures the volume of urine sample, and flat-ended tube uses transparent glass or plastics material
Matter, convenient for observing color, the turbidity etc. of urine sample.
Detailed description of the invention
Fig. 1 is a kind of to save the structural schematic diagram that liquid prepares the pelletizer of urine test sample using urine.
Wherein, 1. Pingdu pipes, 2. pipe lids, 3. coverslips.
Specific embodiment
The following examples illustrate the utility model, but is not intended to limit the scope of the present invention.
Embodiment 1
A kind of urine preservation liquid:
Component is constituted: by mass percentage are as follows: sodium citrate 20g, sodium chloride 0.8g, potassium chloride 0.3g, calcium chloride
0.2g, formaldehyde 244ml, ethyl alcohol 380ml, glacial acetic acid 10g, distilled water 469ml.
Preparation method:
A. sodium chloride 0.8g, potassium chloride 0.3g, calcium chloride 0.2g are weighed by formula, is put into mixer and is stirred
It is even；
B. distilled water 469ml, stirring and dissolving is added by formula ratio into mixture obtained by step A；
C. formaldehyde 244ml, ethyl alcohol 380ml is added by formula ratio into step B acquired solution, is stirred to obtain mixed liquor；
D. sodium citrate 20g, stirring and dissolving mixing is added by formula ratio in the mixed liquor obtained by step C；
E. glacial acetic acid 10g is added into step D acquired solution by formula ratio, adjusts pH value to 6.5.
As shown in Figure 1, a kind of save the pelletizer that liquid prepares urine test sample, including flat-ended tube 1, pipe using urine
Lid 2 and coverslip 3,1 upper end opening of flat-ended tube, lower end closed, material is transparent glass, and the material of the pipe lid 2 is modeling
Material, the volume of the flat-ended tube 1 are 50ml, diameter 30mm, are provided with scale on the outside of tube wall, the diameter of the coverslip 3 is
28mm is lain in a horizontal plane in flat-ended tube 1, and the pipe lid 2 is connected with the upper, open end of flat-ended tube 1 by screw thread.
Using the thin-layer cell smear of the pelletizer production urine sample of urine test sample, cytology inspection is carried out:
Freshly voided urine 20ml is acquired, above-mentioned 20ml urine preservation liquid is added and is uniformly mixed, is divided in 10 pelletizers, covers in equal volume
The good pipe lid 2, marks.Respectively pick and place set 3 days, 1 week, 1 month, 2 months, 4 months and the urine sample after 6 months into
The flat-ended tube 1 is put into centrifuge by row film-making, is centrifugated 5 minutes under the acceleration of gravity of 2000G, described in taking-up
Coverslip 3 is put into flat ware, observes urine cellular morphology, turbidity etc. under the microscope, and acquires image retention.
Experimental result: the urine cell picture that the acquisition of each period is retained is compared:
| Serial number | Time | Form | Turbidity | Dyeing |
| 1 | 3 days | Completely | It is limpid | Basket, Huang |
| 2 | 1 week | Completely | It is limpid | Basket, Huang |
| 3 | 1 month | Completely | It is limpid | Basket, Huang |
| 4 | 2 months | Completely | It is limpid | Basket, Huang |
| 5 | 4 months | Completely | It is limpid | Basket, Huang |
| 6 | 6 months | Completely | It is limpid | Basket, Huang |
Picture is detected according to the urine sample that each period saves, obtains and saves what liquid saved using the utility model urine
Urine cell keeps form stable within each period, and dyeing is clear.
Embodiment 2
A kind of urine preservation liquid:
Component is constituted: by mass percentage are as follows: sodium citrate 12g, sodium chloride 0.5g, potassium chloride 0.2g, calcium chloride
0.1g, formaldehyde 158ml, ethyl alcohol 228ml, glacial acetic acid 7.5g, distilled water 339ml.
Preparation method:
A. sodium chloride 0.5g, potassium chloride 0.2g, calcium chloride 0.1g are weighed by formula, is put into mixer and is stirred
It is even；
B. the distilled water 339ml of formula ratio, stirring and dissolving are added into mixture obtained by step A；
C. formaldehyde 158ml, ethyl alcohol 228ml is added by formula ratio into step B acquired solution, is stirred to obtain mixed liquor；
D. sodium citrate 12g, stirring and dissolving mixing is added by formula ratio in the mixed liquor obtained by step C；
E. glacial acetic acid 7.5g is added into step D acquired solution by formula ratio and adjusts pH value to 7.1.
As shown in Figure 1, a kind of save the pelletizer that liquid prepares urine test sample, including flat-ended tube using above-mentioned urine
1, pipe lid 2 and coverslip 3,1 upper end opening of flat-ended tube, lower end closed, material is transparent plastic, the material of the pipe lid 2
For plastics, the volume of the flat-ended tube 1 is 50ml, and diameter 30mm, tube wall outside is equipped with scale, the diameter of the coverslip 3
It for 28mm, lies in a horizontal plane in flat-ended tube 1, the pipe lid 2 is connected with the upper, open end of flat-ended tube 1 by screw thread.
Using the thin-layer cell smear of the pelletizer production urine sample of urine test sample, cytology inspection is carried out:
Freshly voided urine 20ml is acquired, above-mentioned 20ml urine preservation liquid is added and is uniformly mixed, is divided in 10 pelletizers, covers in equal volume
The good pipe lid 2, marks.Respectively pick and place set 3 days, 1 week, 1 month, 2 months, 4 months and the urine sample after 6 months into
The flat-ended tube 1 is put into centrifuge by row film-making, is centrifugated 5 minutes under the acceleration of gravity of 2000G, described in taking-up
Coverslip 3 is put into flat ware, observes urine cellular morphology, turbidity etc. under the microscope, and acquires image retention.
Experimental result: the urine cell picture that the acquisition of each period is retained is compared:
Picture is detected according to the urine sample that each period saves, obtains and saves what liquid saved using the utility model urine
Urine cell keeps form stable within each period, and dyeing is clear.
Although above having made detailed description to the utility model with generality explanation and specific embodiment,
On the basis of the utility model, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements on the basis of without departing from the spirit of the present invention, belong to the utility model and want
Seek the range of protection.
Claims (4)
1. a kind of urine saves the pelletizer that liquid prepares urine test sample, it is characterised in that: the urine saves liquid preparation
The pelletizer of urine test sample includes flat-ended tube (1), pipe lid (2) and coverslip (3), and the coverslip (3) is horizontal positioned
In flat-ended tube (1), the pipe lid (2) is connected with the upper, open end of flat-ended tube (1) by screw thread.
2. pelletizer according to claim 1, it is characterised in that: the material of the flat-ended tube (1) be transparent glass or
Plastics, the material of the pipe lid (2) are plastics.
3. pelletizer according to claim 1, it is characterised in that: the volume of the flat-ended tube (1) is 50ml, and diameter is
30mm, the diameter of the coverslip (3) are 28mm.
4. pelletizer according to claim 1, it is characterised in that: be equipped with scale on the outside of the tube wall of the flat-ended tube (1).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820932963.9U CN208540656U (en) | 2018-06-15 | 2018-06-15 | A kind of urine preservation liquid prepares the pelletizer of urine test sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820932963.9U CN208540656U (en) | 2018-06-15 | 2018-06-15 | A kind of urine preservation liquid prepares the pelletizer of urine test sample |
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| Publication Number | Publication Date |
|---|---|
| CN208540656U true CN208540656U (en) | 2019-02-26 |
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ID=65422790
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| CN201820932963.9U Active CN208540656U (en) | 2018-06-15 | 2018-06-15 | A kind of urine preservation liquid prepares the pelletizer of urine test sample |
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| Country | Link |
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2018
- 2018-06-15 CN CN201820932963.9U patent/CN208540656U/en active Active
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