CN105454219A - Stationary liquid for preserving pathological tissues - Google Patents
Stationary liquid for preserving pathological tissues Download PDFInfo
- Publication number
- CN105454219A CN105454219A CN201510921898.0A CN201510921898A CN105454219A CN 105454219 A CN105454219 A CN 105454219A CN 201510921898 A CN201510921898 A CN 201510921898A CN 105454219 A CN105454219 A CN 105454219A
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- CN
- China
- Prior art keywords
- fixer
- pathological
- stationary liquid
- tissue
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Abstract
The invention discloses stationary liquid for preserving pathological tissues. The stationary liquid is prepared from, by weight, formalin, Na<2>HPO<4>.12H<2>O, NH<2>PO<4>.2H<2>O, sodium chloride, acetic acid and ethyl alcohol; the mentioned constituents are mixed into a solution to serve as the stationary liquid for preserving a product, and a collected pathological specimen and the stationary liquid are both contained in a container, so that the pathological specimen can be preserved and fixed. By means of the stationary liquid, pathological tissue preservation and fixation can be achieved by one step, the preservation and fixation effect of the pathological tissues is remarkably improved, influence on active substances is reduced, the problem that the medical worker needs to compound stationary liquid temporarily is solved, convenience and rapidness are achieved, compounding is not required, and the stationary liquid can be used just after the container is opened; when the stationary liquid is used for preserving the pathological tissues, the specimen with the fresh tissues, in particular to specimen tissues rich in fibers, can be expanded and fix chromatin rapidly, biological enzyme is not closed, and fixation for section staining is facilitated; the neutral environment of body fluid is simulated, the pathological tissues which are preserved for a long time remain fresh, and the color is not changed. Preservation and fixation of the pathological tissues can be more stable, and it is helpful to improve the pathological section detection effect.
Description
Technical field
The present invention relates to pathological tissue Techniques of preserving field, specifically a kind of pathological tissue preserves fixer.
Background technology
From operating room to pathology department/clinical laboratory's surgery, biopsy, histopathology section office, gastroscope section office etc. need the situation that is fixed tissue or cell etc., stores, keep sample, tradition tissue fixative solution uses formalin, organizes easier chromogenesis to precipitate through fixing outmoded of formaldehyde.
Summary of the invention
The pathological tissue of a kind of plug and play, good fixing effect is the object of the present invention is to provide to preserve fixer, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
A kind of pathological tissue preserves fixer, comprises: formalin: 80-120ml, Na according to the raw material of weight portion
2hPO
412H
2o:11-15g, NaH
2pO
42H
2o:2-2.3g, sodium chloride: 8-10g, acetic acid: 25-35ml, ethanol: 45-55ml.
As the further scheme of the present invention: described pathological tissue preserves fixer, comprises: formalin: 90-110ml, Na according to the raw material of weight portion
2hPO
412H
2o:12-14g, NaH
2pO
42H
2o:2.1-2.2g, sodium chloride: 8.5-9.5g, acetic acid: 28-32ml, ethanol: 48-52ml.
As the further scheme of the present invention: described pathological tissue preserves fixer, comprises: formalin: 100ml, Na according to the raw material of weight portion
2hPO
412H
2o:13g, NaH
2pO
42H
2o:2.14g, sodium chloride: 9g, acetic acid: 30ml, ethanol: 50ml.
As the further scheme of the present invention: the pH value that described pathological tissue preserves fixer is 7.0 ± 0.05.
As the present invention's further scheme: described pathological tissue preserves fixer, in use according to organizing size to select fixer specification, capacity, tear the sealing of fixer reagent, open valve bag or open sample storing bottle, put in 5min by vitro tissue, fixer and tissue specimen volume ratio are not less than 2 times, the complete submergence tissue specimen of fixer, be tamping valve bag, the set time is 2-12h.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention makes the fixing realization of pathology preservation settle at one go, significantly improves preservation and the fixed effect of pathological tissue, and reduces active substance impact, solve the problem that medical matters people needs Extemporaneous fixer, convenient and swift, without the need to preparing, and plug and play; Flesh tissue sample especially rich fibrous sample tissue bulking can be made when preserving pathological tissue, fix chromatin rapidly, and can not biology enzyme be closed, be conducive to fixing section statining; The neutral environment of simulated body fluid, the fresh nondiscolouring of long-term preservation pathological tissue.Make the preservation of pathological tissue and fix more stable, contributing to the Detection results improving pathological section.
Embodiment
Be described in more detail below in conjunction with the technical scheme of embodiment to this patent.
Embodiment 1
A kind of pathological tissue preserves fixer, comprises: formalin: 80ml, Na according to the raw material of weight portion
2hPO
412H
2o:11g, NaH
2pO
42H
2o:2g, sodium chloride: 8g, acetic acid: 25ml, ethanol: 45ml; Above composition mixes solution, preserves fixer, the Pathologic specimen of collection and fixer are all contained in container as product, in order to preserve, to fix Pathologic specimen.
Described pathological tissue preserves fixer in use, fixer specification, capacity is selected according to organizing size, tear the sealing of fixer reagent, open valve bag or open sample storing bottle, put in 5min by vitro tissue, fixer and tissue specimen volume ratio are not less than 2 times, the complete submergence tissue specimen of fixer, be tamping valve bag, the set time is 2h.
Embodiment 2
A kind of pathological tissue preserves fixer, comprises: formalin: 90ml, Na according to the raw material of weight portion
2hPO
412H
2o:12g, NaH
2pO
42H
2o:2.1g, sodium chloride: 8.5g, acetic acid: 28ml, ethanol: 48ml; Above composition mixes solution, preserves fixer, the Pathologic specimen of collection and fixer are all contained in container as product, in order to preserve, to fix Pathologic specimen.
Described pathological tissue preserves fixer in use, fixer specification, capacity is selected according to organizing size, tear the sealing of fixer reagent, open valve bag (or opening sample storing bottle), put in 5min by vitro tissue, fixer and tissue specimen volume ratio are not less than 2 times, the complete submergence tissue specimen of fixer, be tamping valve bag, the set time is 5h.
Embodiment 3
A kind of pathological tissue preserves fixer, comprises: formalin: 100ml, Na according to the raw material of weight portion
2hPO
412H
2o:13g, NaH
2pO
42H
2o:2.14g, sodium chloride: 9g, acetic acid: 30ml, ethanol: 50ml; Above composition mixes solution, preserves fixer, the Pathologic specimen of collection and fixer are all contained in container as product, in order to preserve, to fix Pathologic specimen.
Described pathological tissue preserves fixer in use, fixer specification, capacity is selected according to organizing size, tear the sealing of fixer reagent, open valve bag (or opening sample storing bottle), put in 5min by vitro tissue, fixer and tissue specimen volume ratio are not less than 2 times, the complete submergence tissue specimen of fixer, be tamping valve bag, the set time is 7h.
Embodiment 4
A kind of pathological tissue preserves fixer, comprises: formalin: 110ml, Na according to the raw material of weight portion
2hPO
412H
2o:14g, NaH
2pO
42H
2o:2.2g, sodium chloride: 9.5g, acetic acid: 32ml, ethanol: 52ml; Above composition mixes solution, preserves fixer, the Pathologic specimen of collection and fixer are all contained in container as product, in order to preserve, to fix Pathologic specimen.
Described pathological tissue preserves fixer in use, fixer specification, capacity is selected according to organizing size, tear the sealing of fixer reagent, open valve bag (or opening sample storing bottle), put in 5min by vitro tissue, fixer and tissue specimen volume ratio are not less than 2 times, the complete submergence tissue specimen of fixer, be tamping valve bag, the set time is 9h.
Embodiment 5
A kind of pathological tissue preserves fixer, comprises: formalin: 120ml, Na according to the raw material of weight portion
2hPO
412H
2o:15g, NaH
2pO
42H
2o:2.3g, sodium chloride: 10g, acetic acid: 35ml, ethanol: 55ml; Above composition mixes solution, preserves fixer, the Pathologic specimen of collection and fixer are all contained in container as product, in order to preserve, to fix Pathologic specimen.
Described pathological tissue preserves fixer in use, fixer specification, capacity is selected according to organizing size, tear the sealing of fixer reagent, open valve bag (or opening sample storing bottle), put in 5min by vitro tissue, fixer and tissue specimen volume ratio are not less than 2 times, the complete submergence tissue specimen of fixer, be tamping valve bag, the set time is 12h.
The present invention makes the fixing realization of pathology preservation settle at one go, significantly improves preservation and the fixed effect of pathological tissue, and reduces active substance impact, solve the problem that medical matters people needs Extemporaneous fixer, convenient and swift, without the need to preparing, and plug and play; Flesh tissue sample especially rich fibrous sample tissue bulking can be made when preserving pathological tissue, fix chromatin rapidly, and can not biology enzyme be closed, be conducive to fixing section statining; The neutral environment of simulated body fluid, the fresh nondiscolouring of long-term preservation pathological tissue.Make the preservation of pathological tissue and fix more stable, contributing to the Detection results improving pathological section.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment, in the ken that one skilled in the relevant art possesses, various change can also be made under the prerequisite not departing from this patent aim.
Claims (5)
1. pathological tissue preserves a fixer, it is characterized in that, comprises: formalin: 80-120ml, Na according to the raw material of weight portion
2hPO
412H
2o:11-15g, NaH
2pO
42H
2o:2-2.3g, sodium chloride: 8-10g, acetic acid: 25-35ml, ethanol: 45-55ml.
2. institute's pathological tissue according to claim 1 preserves fixer, it is characterized in that, comprises: formalin: 90-110ml, Na according to the raw material of weight portion
2hPO
412H
2o:12-14g, NaH
2pO
42H
2o:2.1-2.2g, sodium chloride: 8.5-9.5g, acetic acid: 28-32ml, ethanol: 48-52ml.
3. preserve fixer according to the arbitrary described pathological tissue of claim 1-2, it is characterized in that, comprise according to the raw material of weight portion: formalin: 100ml, Na
2hPO
412H
2o:13g, NaH
2pO
42H
2o:2.14g, sodium chloride: 9g, acetic acid: 30ml, ethanol: 50ml.
4. preserve fixer according to the arbitrary described pathological tissue of claim 1-2, it is characterized in that, the pH value that described pathological tissue preserves fixer is 7.0 ± 0.05.
5. preserve fixer according to the arbitrary described pathological tissue of claim 1-2, it is characterized in that, in use according to organizing size to select fixer specification, capacity, tear the sealing of fixer reagent, open valve bag or open sample storing bottle, in vitro tissue is put in 5min, fixer and tissue specimen volume ratio are not less than 2 times, the complete submergence tissue specimen of fixer, is tamping valve bag, and the set time is 2-12h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510921898.0A CN105454219A (en) | 2015-12-14 | 2015-12-14 | Stationary liquid for preserving pathological tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510921898.0A CN105454219A (en) | 2015-12-14 | 2015-12-14 | Stationary liquid for preserving pathological tissues |
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| Publication Number | Publication Date |
|---|---|
| CN105454219A true CN105454219A (en) | 2016-04-06 |
Family
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| CN201510921898.0A Pending CN105454219A (en) | 2015-12-14 | 2015-12-14 | Stationary liquid for preserving pathological tissues |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689120A (en) * | 2017-03-24 | 2017-05-24 | 南阳医学高等专科学校 | Preserving stationary liquid for pathological tissues |
| CN109187148A (en) * | 2018-08-31 | 2019-01-11 | 江苏世泰实验器材有限公司 | A kind of pathological tissue specimen fixer |
| CN110720452A (en) * | 2019-11-05 | 2020-01-24 | 南通大学 | Method for optimizing preservation of pathological gross specimens |
| CN111397997A (en) * | 2020-04-26 | 2020-07-10 | 中烨(山东)检验检测有限公司 | Tissue fixing liquid for fixing fresh tissue sample |
| CN111670896A (en) * | 2020-04-28 | 2020-09-18 | 浙江大学医学院附属妇产科医院 | Neutral buffer tissue fixing solution |
Citations (2)
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|---|---|---|---|---|
| WO2000000813A1 (en) * | 1998-06-30 | 2000-01-06 | Lamina, Inc. | Cytological and histological fixative composition and methods of use |
| CN104663647A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Heart tissue fixing agent as well as preparation method and application of heart tissue fixing agent |
-
2015
- 2015-12-14 CN CN201510921898.0A patent/CN105454219A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000000813A1 (en) * | 1998-06-30 | 2000-01-06 | Lamina, Inc. | Cytological and histological fixative composition and methods of use |
| CN104663647A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Heart tissue fixing agent as well as preparation method and application of heart tissue fixing agent |
Non-Patent Citations (3)
| Title |
|---|
| 刘力华等: ""病理常规制片技术各环节注意事项"", 《卫生职业教育》 * |
| 唐从国: ""几种常用病理组织固定液的比较"", 《中国现代医药杂志》 * |
| 曹江: ""介绍一种淋巴结组织切片的优良固定液"", 《浙江肿瘤通讯》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689120A (en) * | 2017-03-24 | 2017-05-24 | 南阳医学高等专科学校 | Preserving stationary liquid for pathological tissues |
| CN109187148A (en) * | 2018-08-31 | 2019-01-11 | 江苏世泰实验器材有限公司 | A kind of pathological tissue specimen fixer |
| CN110720452A (en) * | 2019-11-05 | 2020-01-24 | 南通大学 | Method for optimizing preservation of pathological gross specimens |
| CN111397997A (en) * | 2020-04-26 | 2020-07-10 | 中烨(山东)检验检测有限公司 | Tissue fixing liquid for fixing fresh tissue sample |
| CN111670896A (en) * | 2020-04-28 | 2020-09-18 | 浙江大学医学院附属妇产科医院 | Neutral buffer tissue fixing solution |
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Application publication date: 20160406 |