CN102048990A - Quality control method for Chinese medicinal preparation Qinggong Shoutao pills - Google Patents
Quality control method for Chinese medicinal preparation Qinggong Shoutao pills Download PDFInfo
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Abstract
The invention relates to a quality control method for a Chinese medicinal preparation Qinggong Shoutao pills, which comprises the following steps of: performing microscopical identification; and identifying whether the prescription of the Qinggong Shoutao pills comprises components such as Chinese angelica, medlar and dwarf lilyturf tuber or not by thin layer chromatography. On the basis of the primary standard, the microscopical identification method for the Qinggong Shoutao pills is modified, the descriptions of microscopical characteristics of ginseng and rehmannia root in the primary standard are deleted, the thin layer identification method for the Chinese angelica, the medlar and the dwarf lilyturf tuber is added, so that counterfeit medicines and inferior medicines can be more accurately inspected, the quality control of the medicines is improved, and the method has great significance for the modernization and internationalization of traditional Chinese medicines.
Description
Technical field
The invention belongs to technical field of Chinese medicines, relate to the detection method of Chinese medicine, the method for quality control of especially a kind of Chinese medicine preparation clearing heat in the pericardium birthday peaches ball.
Background technology
Clearing heat in the pericardium birthday peaches ball is made up of Testis Et penis Equi, Penis et testis cervi, Testis et Pentis Canis, Fructus Lycii, Radix Ginseng, Radix Asparagi, Radix Ophiopogonis, Radix Rehmanniae, Radix Angelicae Sinensis, Fructus Alpiniae Oxyphyllae (processed with salt), silkworm excrement, Semen Ziziphi Spinosae (stir-fry), Partition in Fructus Juglandis (parching to brown), its preparation method is: above 13 flavors, be ground into fine powder, sieve, mixing, every 100g powder adds refined honey 150～170g and makes big honeyed pills promptly, clearing heat in the pericardium birthday peaches ball has kidney-tonifying sperm-generating, and benefit unit is strong, and is dizzy tired due to the aging that is used to suffer from a deficiency of the kidney, hypomnesis, soreness of the waist and knees, Hiccough and deaf, dim eyesight is shed tears, frequent urination at night, diseases such as dripping urination.
Though still there is not the international standard of plant medicine at present in the world, the trend of Chinese medicine standard but the south east asia of the U.S., European Union, China and tradition outlet Chinese medicine is improved, in the case, need to propose to be fit to the standard of China's product quality to adapt to international standard, it is historical that China has the Chinese medicine in thousands of years to use, countries in the world are many Chinese medicine standards with reference to China in working out corresponding plant amedica target level of product quality, so quality standard perfect, that improve Chinese medicine is more extremely urgent.
Birthday peaches ball existing quality standard in the clearing heat in the pericardium does not have the concrete authentication method about each composition, just described from microstructure, the qualitative and quantitative detection that is unfavorable for so very much this medicine, bring very big difficulty for the quality control of this medicine, according to the retrieval, do not have the relevant patent about the method for quality control of clearing heat in the pericardium birthday peaches ball at present, existing patent mainly concentrates on the important prescription and preparation method of clearing heat in the pericardium birthday peaches ball.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, a kind of method of quality control of Chinese medicine preparation clearing heat in the pericardium birthday peaches ball that can the qualitative and quantitative analysis ingredient is provided.
The objective of the invention is to be achieved through the following technical solutions:
The method of quality control of a kind of Chinese medicine preparation clearing heat in the pericardium birthday peaches ball, step is:
(1) microscopical identification;
(2) adopt thin layer chromatography,, differentiate the Radix Angelicae Sinensis in the prescription with when being classified as control medicinal material;
(3) adopting thin layer chromatography, is control medicinal material with the Fructus Lycii, differentiates the Fructus Lycii in the prescription;
(4) adopting thin layer chromatography, is control medicinal material with the Radix Ophiopogonis, differentiates the Radix Ophiopogonis in the prescription.
And described microscopical identification step is: put microscopically and observe: plant the faint yellow extremely yellow of skin stone cell, and wall thickness, wavy bending, cell contains brown thing; Endotesta prothenchyma (of wood) yellowish-brown or rufous, wall is thicker, non-lignify, cell contains siliceous; It is in blocks to plant the skin palisade cells, yellowish-brown or light red brown, and the class polygon is arranged closely, and long column shape is seen in the side, long 60～145 μ m; Contain numerous tiny calcium oxalate cluster crystals in the indigested mesophyll tissue fragment, diameter 5～20 μ m.
And described thin layer chromatography differentiates that the method for the Radix Angelicae Sinensis composition in the prescription is:
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, shred, the 20ml that adds diethyl ether, supersound process 15 minutes filters, and filtrate volatilizes, and residue adds methanol 3ml makes dissolving, as need testing solution;
2. the preparation of control medicinal material solution: get Radix Angelicae Sinensis control medicinal material 0.5g, the 20ml that adds diethyl ether, supersound process 15 minutes filters, and filtrate volatilizes, and residue adds methanol 3ml makes dissolving, in contrast medical material solution;
3. thin layer condition and result: thin layer chromatography test, draw each 2～4 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with normal hexane: ethyl acetate=4: 1 is developing solvent, launches, and takes out, dry, put under the ultra-violet lamp 365nm and inspect, inspect in the test sample chromatograph with the corresponding position of control medicinal material chromatograph on whether show the fluorescence speckle of same color, determine whether contain the Radix Angelicae Sinensis composition in the test agent.
And described thin layer chromatography differentiates that the method for the Fructus Lycii composition in the prescription is:
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, shred, add water 30ml, heated and boiled 15 minutes, put coldly, centrifugal 5 minutes of 3000rpm gets supernatant, extract 2 times with the ethyl acetate jolting, each 20ml, combined ethyl acetate liquid, evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution;
2. the preparation of control medicinal material solution: get Fructus Lycii control medicinal material 1g, add water 30ml, heated and boiled 15 minutes is put cold, centrifugal 5 minutes of 3000rpm, get supernatant, extract 2 times, each 20ml with the ethyl acetate jolting, combined ethyl acetate liquid, evaporate to dryness, residue add methanol 1ml makes dissolving, in contrast medical material solution;
3. thin layer condition and result: thin layer chromatography test, draw each 4～6 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform: ethyl acetate: formic acid=2: 3: 1 is developing solvent, launches, and takes out, dry, put under the ultra-violet lamp 365nm and inspect, inspect in the test sample chromatograph with the corresponding position of control medicinal material chromatograph on whether show the fluorescence speckle of same color, determine whether contain the Fructus Lycii composition in the test agent.
And, described thin layer chromatography differentiate in the prescription Radix Ophiopogonis composition method be:
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, add methanol 30ml, supersound process 30 minutes, put cold, filter, filtrate evaporate to dryness, residue add water 25ml makes dissolving, extract 2 times with the chloroform jolting, each 20ml merges chloroform, evaporate to dryness, residue adds methanol 2ml makes dissolving, as need testing solution;
2. the preparation of control medicinal material solution: get control medicinal material 2g Radix Ophiopogonis, add methanol 30ml, supersound process 30 minutes, put cold, filter, filtrate evaporate to dryness, residue add water 25ml makes dissolving, extract 2 times with the chloroform jolting, each 20ml merges chloroform liquid, evaporate to dryness, residue adds methanol 2ml makes dissolving, in contrast medical material solution;
3. thin layer condition and result: thin layer chromatography test, draw each 4～6 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform: acetone=4: 1 is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, it is clear to be heated to the speckle colour developing, place daylight and ultra-violet lamp 365nm to detect down, inspect in the test sample chromatograph with the corresponding position of control medicinal material chromatograph on whether show the speckle or the fluorescence speckle of same color respectively, determine whether contain composition Radix Ophiopogonis in the test agent.
Advantage of the present invention and good effect are:
1, the clearing heat in the pericardium of the present invention birthday peaches ball is on the primary standard basis, revised the microscopical identification method, Radix Angelicae Sinensis, Fructus Lycii, the thin layer discrimination method of Radix Ophiopogonis have been increased, can check out counterfeit drug and substandard drug more exactly after the modification, improved the quality control of medicine, the modernization of Chinese medicine and Chinese medicine are gone abroad to be significant.
2, in the birthday peaches ball primary standard of the clearing heat in the pericardium, adopt the microscopical identification method that Fructus Lycii, Fructus Alpiniae Oxyphyllae, Radix Ginseng and Radix Rehmanniae are described, check discovery, because Radix Ginseng, after the special processing of ecliptic longitude, microscopic features separately are not obvious, cause its feature to be not easy to observe, so deleted the description of the microscopic features of Radix Ginseng, Radix Rehmanniae in the primary standard, the present invention revises part microscopic features in the primary standard, has increased the feature description of Semen Ziziphi Spinosae, silkworm excrement in the prescription simultaneously.
Description of drawings
200601), sample 2 (lot number: 200602), sample 3 (lot number: 200603), the Radix Angelicae Sinensis control medicinal material Fig. 1 inspects figure for the present invention detects thin-layer chromatogram for Radix Angelicae Sinensis in the test agent at ultra-violet lamp (365nm), and wherein band is followed successively by from left to right: negative control, sample 1 (lot number:;
200601), sample 2 (lot number: 200602), sample 3 (lot number: 200603), the Fructus Lycii control medicinal material Fig. 2 detects chromatogram at ultra-violet lamp (365nm) for the present invention for Fructus Lycii in the test agent, and wherein band is followed successively by from left to right: negative control, sample 1 (lot number:;
Fig. 3 inspects figure for detecting thin-layer chromatogram Radix Ophiopogonis in the test agent for the present invention under daylight, wherein band is followed successively by from left to right: negative control, sample 1 (lot number: 200601,6 μ l), sample 2 (lot number: 200602,6 μ l), sample 3 (lot number: 200603,6 μ l), Radix Ophiopogonis control medicinal material, sample 1 (lot number: 200601,2 μ l);
Fig. 4 inspects under ultra-violet lamp (365nm) for detecting thin-layer chromatogram Radix Ophiopogonis in the test agent for the present invention, wherein, wherein band is followed successively by from left to right: negative control, sample 1 (lot number: 200601,6 μ l), sample 2 (lot number: 200602,6 μ l), sample 3 (lot number: 200603,6 μ l), Radix Ophiopogonis control medicinal material, sample 1 (lot number: 200601,2 μ l).
The specific embodiment
Below in conjunction with embodiment, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
The method of quality control of Chinese medicine preparation clearing heat in the pericardium birthday peaches ball, the step of its method is:
(1) microscopical identification:
Putting microscopically observes: plant the faint yellow extremely yellow of skin stone cell, and wall thickness, wavy bending, cell contains brown thing (Fructus Lycii).Endotesta prothenchyma (of wood) yellowish-brown or rufous, wall is thicker, non-lignify, cell contains siliceous (Fructus Alpiniae Oxyphyllae).It is in blocks to plant the skin palisade cells, yellowish-brown or light red brown, and the class polygon is arranged closely, and long column shape is seen in the side, long 60～145 μ m (Semen Ziziphi Spinosae).Contain numerous tiny calcium oxalate cluster crystals in the indigested mesophyll tissue fragment, diameter 5～20 μ m (silkworm excrement).
(2) Radix Angelicae Sinensis: with reference to " related content of Chinese pharmacopoeia version in 2005 is worked out.Adopting thin layer chromatography, is contrast with the Radix Angelicae Sinensis control medicinal material, differentiates the Radix Angelicae Sinensis in the prescription.
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, shred, the 20ml that adds diethyl ether, supersound process 15 minutes filters, and filtrate volatilizes, and residue adds methanol 3ml makes dissolving, as need testing solution.
2. the preparation of control medicinal material solution: get Radix Angelicae Sinensis control medicinal material 0.5g, the 20ml that adds diethyl ether, supersound process 15 minutes filters, and filtrate volatilizes, and residue adds methanol 3ml makes dissolving, in contrast medical material solution.
3. the preparation of negative sample solution: by the prescription proportioning, get other flavour of a drug except that Radix Angelicae Sinensis, make pill, make negative sample solution by above-mentioned need testing solution preparation method again by former medicine preparation technology.
4. thin layer condition and result: according to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, draw each 2～4 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, with normal hexane: ethyl acetate=4: 1 is developing solvent, launch, take out, dry, put under the ultra-violet lamp 365nm and inspect.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color, negative sample solution is noiseless, measurement result is seen Fig. 1.
(3) Fructus Lycii: with reference to " related content of Chinese pharmacopoeia version in 2005 is worked out.Adopting thin layer chromatography, is control medicinal material with the Fructus Lycii, differentiates the Fructus Lycii in the prescription.
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, shred, add water 30ml, heated and boiled 15 minutes, put coldly, centrifugal 5 minutes (3000rpm) gets supernatant, extract 2 times with the ethyl acetate jolting, each 20ml, combined ethyl acetate liquid, evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution.
2. the preparation of control medicinal material solution: get Fructus Lycii control medicinal material 1g, add water 30ml, heated and boiled 15 minutes is put cold, centrifugal 5 minutes (3000rpm), get supernatant, extract 2 times, each 20ml with the ethyl acetate jolting, combined ethyl acetate liquid, evaporate to dryness, residue add methanol 1ml makes dissolving, in contrast medical material solution.
3. the preparation of negative sample solution: by the prescription proportioning, get other flavour of a drug except that Fructus Lycii, make pill, make negative sample solution by above-mentioned need testing solution preparation method again by former medicine preparation technology.
4. thin layer condition and result: according to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, draw each 4～6 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform-ethyl acetate-formic acid (2: 3: 1) is developing solvent, launch, take out, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color, negative sample solution is noiseless.Measurement result is seen Fig. 2.
(4) Radix Ophiopogonis: with reference to " related content of Chinese pharmacopoeia version in 2005 is worked out.Adopting thin layer chromatography, is control medicinal material with the Radix Ophiopogonis, differentiates the Radix Ophiopogonis in the prescription.
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, add methanol 30ml, supersound process 30 minutes, put cold, filter, filtrate evaporate to dryness, residue add water 25ml makes dissolving, extract 2 times with the chloroform jolting, each 20ml merges chloroform, evaporate to dryness, residue adds methanol 2ml makes dissolving, as need testing solution.
2. the preparation of control medicinal material solution: get control medicinal material 2g Radix Ophiopogonis, add methanol 30ml, supersound process 30 minutes, put cold, filter, filtrate evaporate to dryness, residue add water 25ml makes dissolving, extract 2 times with the chloroform jolting, each 20ml merges chloroform liquid, evaporate to dryness, residue adds methanol 2ml makes dissolving, in contrast medical material solution.
3. the preparation of negative sample solution: by the prescription proportioning, get other flavour of a drug except that Radix Ophiopogonis, make pill, make negative sample solution by above-mentioned need testing solution preparation method again by former medicine preparation technology.
4. thin layer condition and result: according to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, draw each 4～6 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform: acetone=4: 1 is developing solvent, launches, and takes out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing, puts respectively under daylight and the ultra-violet lamp (365nm) and inspects.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle or the fluorescence speckle of same color respectively.Negative sample solution is noiseless.Measurement result is seen Fig. 3, Fig. 4, through finding that relatively inspect under the daylight, effect is better, so the condition of inspecting under the ultra-violet lamp (365nm) is not listed in the standard body.
Claims (5)
1. the method for quality control of a Chinese medicine preparation clearing heat in the pericardium birthday peaches ball, it is characterized in that: the step of its method is:
(1) microscopical identification;
(2) adopt thin layer chromatography,, differentiate the Radix Angelicae Sinensis in the prescription with when being classified as control medicinal material;
(3) adopting thin layer chromatography, is control medicinal material with the Fructus Lycii, differentiates the Fructus Lycii in the prescription;
(4) adopting thin layer chromatography, is control medicinal material with the Radix Ophiopogonis, differentiates the Radix Ophiopogonis in the prescription.
2. the method for quality control of Chinese medicine preparation according to claim 1 clearing heat in the pericardium birthday peaches ball is characterized in that: described microscopical identification step is: put microscopically and observe: plant the faint yellow extremely yellow of skin stone cell, and wall thickness, wavy bending, cell contains brown thing; Endotesta prothenchyma (of wood) yellowish-brown or rufous, wall is thicker, non-lignify, cell contains siliceous; It is in blocks to plant the skin palisade cells, yellowish-brown or light red brown, and the class polygon is arranged closely, and long column shape is seen in the side, long 60～145 μ m; Contain numerous tiny calcium oxalate cluster crystals in the indigested mesophyll tissue fragment, diameter 5～20 μ m.
3. the method for quality control of Chinese medicine preparation according to claim 1 clearing heat in the pericardium birthday peaches ball is characterized in that: described thin layer chromatography differentiates that the method for the Radix Angelicae Sinensis composition in the prescription is:
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, shred, the 20ml that adds diethyl ether, supersound process 15 minutes filters, and filtrate volatilizes, and residue adds methanol 3ml makes dissolving, as need testing solution;
2. the preparation of control medicinal material solution: get Radix Angelicae Sinensis control medicinal material 0.5g, the 20ml that adds diethyl ether, supersound process 15 minutes filters, and filtrate volatilizes, and residue adds methanol 3ml makes dissolving, in contrast medical material solution;
3. thin layer condition and result: thin layer chromatography test, draw each 2～4 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with normal hexane: ethyl acetate=4: 1 is developing solvent, launches, and takes out, dry, put under the ultra-violet lamp 365nm and inspect, inspect in the test sample chromatograph with the corresponding position of control medicinal material chromatograph on whether show the fluorescence speckle of same color, determine whether contain the Radix Angelicae Sinensis composition in the test agent.
4. the method for quality control of Chinese medicine preparation according to claim 1 clearing heat in the pericardium birthday peaches ball is characterized in that: described thin layer chromatography differentiates that the method for the Fructus Lycii composition in the prescription is:
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, shred, add water 30ml, heated and boiled 15 minutes, put coldly, centrifugal 5 minutes of 3000rpm gets supernatant, extract 2 times with the ethyl acetate jolting, each 20ml, combined ethyl acetate liquid, evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution;
2. the preparation of control medicinal material solution: get Fructus Lycii control medicinal material 1g, add water 30ml, heated and boiled 15 minutes is put cold, centrifugal 5 minutes of 3000rpm, get supernatant, extract 2 times, each 20ml with the ethyl acetate jolting, combined ethyl acetate liquid, evaporate to dryness, residue add methanol 1ml makes dissolving, in contrast medical material solution;
3. thin layer condition and result: thin layer chromatography test, draw each 4～6 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform: ethyl acetate: formic acid=2: 3: 1 is developing solvent, launches, and takes out, dry, put under the ultra-violet lamp 365nm and inspect, inspect in the test sample chromatograph with the corresponding position of control medicinal material chromatograph on whether show the fluorescence speckle of same color, determine whether contain the Fructus Lycii composition in the test agent.
5. the method for quality control of Chinese medicine preparation according to claim 1 clearing heat in the pericardium birthday peaches ball is characterized in that: described thin layer chromatography differentiate in the prescription Radix Ophiopogonis composition method be:
1. the preparation of need testing solution: get clearing heat in the pericardium birthday peaches ball sample 9g, add methanol 30ml, supersound process 30 minutes, put cold, filter, filtrate evaporate to dryness, residue add water 25ml makes dissolving, extract 2 times with the chloroform jolting, each 20ml merges chloroform, evaporate to dryness, residue adds methanol 2ml makes dissolving, as need testing solution;
2. the preparation of control medicinal material solution: get control medicinal material 2g Radix Ophiopogonis, add methanol 30ml, supersound process 30 minutes, put cold, filter, filtrate evaporate to dryness, residue add water 25ml makes dissolving, extract 2 times with the chloroform jolting, each 20ml merges chloroform liquid, evaporate to dryness, residue adds methanol 2ml makes dissolving, in contrast medical material solution;
3. thin layer condition and result: thin layer chromatography test, draw each 4～6 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform: acetone=4: 1 is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, it is clear to be heated to the speckle colour developing, place daylight and ultra-violet lamp 365nm to detect down, inspect in the test sample chromatograph with the corresponding position of control medicinal material chromatograph on whether show the speckle or the fluorescence speckle of same color respectively, determine whether contain composition Radix Ophiopogonis in the test agent.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109100431A (en) * | 2017-12-27 | 2018-12-28 | 天津中新药业集团股份有限公司达仁堂制药厂 | A kind of effective substance research method of palace of the Qing Dynasty Shoutao pills |
| CN109498768A (en) * | 2017-12-27 | 2019-03-22 | 天津中新药业集团股份有限公司达仁堂制药厂 | Application of the palace of the Qing Dynasty Shoutao pills in preparation treatment improvement decrease of memory, amnesia, cognitive disorder drug |
| CN110426486A (en) * | 2019-08-01 | 2019-11-08 | 正大青春宝药业有限公司 | The discrimination method of Zhejiang Radix Ophiopogonis in Chinese materia medica preparation |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1256978C (en) * | 2003-12-23 | 2006-05-24 | 天津中新药业集团股份有限公司达仁堂制药厂 | Medicine for tonifying kidney, promoting generation of vital essence and reinforcing primordial qi, and strengthening and its preparing method |
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2009
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109100431A (en) * | 2017-12-27 | 2018-12-28 | 天津中新药业集团股份有限公司达仁堂制药厂 | A kind of effective substance research method of palace of the Qing Dynasty Shoutao pills |
| CN109498768A (en) * | 2017-12-27 | 2019-03-22 | 天津中新药业集团股份有限公司达仁堂制药厂 | Application of the palace of the Qing Dynasty Shoutao pills in preparation treatment improvement decrease of memory, amnesia, cognitive disorder drug |
| CN109100431B (en) * | 2017-12-27 | 2021-08-10 | 天津中新药业集团股份有限公司达仁堂制药厂 | Basic research method of drug effect substances of Qinggong Shoutao pills |
| CN110426486A (en) * | 2019-08-01 | 2019-11-08 | 正大青春宝药业有限公司 | The discrimination method of Zhejiang Radix Ophiopogonis in Chinese materia medica preparation |
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Address after: No.21, 10th Street, Binhai New Area Development Zone, Tianjin 300457 Patentee after: Jinyao Darentang Group Co., Ltd. Darentang Pharmaceutical Factory Address before: 300457 No. tenth, 21 Avenue, Tianjin economic and Technological Development Zone Patentee before: DARENTANG PHARMACEUTICAL FACTORY, TIANJIN ZHONGXIN PHARMACEUTICAL GROUP Co.,Ltd. |
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