CN102697171A - Elaeagnus conferta roxb extract and application thereof - Google Patents

Abstract

The invention relates to elaeagnus conferta roxb extract and application thereof and belongs to the technical field of cigarette production. The elaeagnus conferta roxb extract can be obtained by the following steps: a, immersing just-picked elaeagnus conferta roxbs in 95 percent ethanol by a ratio of 1:5 in preparation; b, extracting 1 kg immersed elaeagnus conferta roxbs and crushing by a machine; c, obtaining 2 kg pectinase to dissolve in 5 kg water, then filtering to prepare enzymatic hydrolyzate for preparation; d, adding the enzymatic hydrolyzate in the crushed elaeagnus conferta roxbs, and then collecting the filtering liquid by filtering the elaeagnus conferta roxbs in a hot reflux at the temperature of 45-55 degrees for 2 h; e, additionally adding 3 kg pure water, and then collecting the filtering liquid again by filtering in a hot reflux at the temperature of 75-85 degrees for 1 h; and f, combining the filtering liquid obtained from the step d and e together, and then obtaining the elaeagnus conferta roxb tincture through the compression and concentration of the filtering liquid. As for the application, the elaeagnus conferta roxb tincture, by weight ratio, about 0.1-0.3 percent is applied to a 100 g cigarette formula leaf group. Moreover, the advantages of improved aroma concentration and richness are provided, and the effects of inhibiting mixed gas and slightly improving the stimulation and aftertaste are also improved.

Description

A kind of elaeagnus conferta fruits extract and application thereof

Technical field:

The present invention relates to a kind of elaeagnus conferta fruits extract and application thereof, belong to the production of cigarettes technical field.

Background technology:

The development low-coke tar cigarette is the demand of consumer, tobacco business, and the reduction of tar content in cigarette causes smoke thin out, and fragrance weakens, and the consumer is difficult to be accepted.So when reducing coke tar in cigarette, exploitation natural function property additive is very necessary.

Elaeagnus conferta fruits (ElatagnuscontetaRoxb.) has another name called close colored thorny elaeagnus, southern thorny elaeagnus, rattan thorny elaeagnus; Xishuangbanna, Yunnan is big fruit thorny elaeagnus; It is the ripening fruits of Elaeangnaceae plant honey flower thorny elaeagnus; Be the perennial evergreen climbing shrub, be distributed in Geothermal Belt rainforest and Yunnan Province of China south and south Guangxi such as Vietnam, Malaysia, India.Fruits nutrition is abundant, and edible rate is 73.3-91%, and per 100 gram fresh fruits are moisture 90.6%, thick protein 2.45%, crude fat 2.8%, total reducing sugar 5.1%, total acid 1.45% etc.Elaeagnus conferta fruits has higher medical value, effects such as Bearberry Extract antidiarrheal, antibechic, detoxifcation, and modern pharmacology proves, feeds mouse with elaeagnus conferta fruits, and chronic bronchitis is had certain curative effect.The organic acid that fruit contains is after concentrating, and for oral administration have inhibitory action to intestinal bacterium.

The present invention has carried out the elaeagnus conferta fruits Study on Extraction Method, extracts preparation elaeagnus conferta fruits tincture as cigarette additive, adopts high resolution mass spectrum (HRMS) to analyze to its volatile ingredient, and has carried out cigarette and added test, sensory evaluating smoking's experiment.Through the document retrieval, relevant its application study aspect tobacco does not appear in the newspapers.

Summary of the invention:

The object of the present invention is to provide a kind of elaeagnus conferta fruits extract and application thereof.

The present invention adopts cold soaking extraction, enzymolysis, the concentrated elaeagnus conferta fruits extract for preparing with fresh elaeagnus conferta fruits.Through the Headspace-GC-MS instrument elaeagnus conferta fruits volatile ingredient is analyzed; Through MassLynx V4.1 chem workstation data handling system; In Nist2008 and WILYL standard spectrum storehouse, retrieve, carry out spectrum elucidation, confirm its chemical composition in conjunction with pertinent literature.Quantitative analysis is tried to achieve the relative percentage composition of each chemical composition respectively through area normalization method, and recall rate is 59.28%.Elaeagnus conferta fruits extract of the present invention obtains through following steps:

A. the elaeagnus conferta fruits that will just pluck is immersed in the ratio of 1:5 in 95% the ethanol, and is subsequent use;

B. take by weighing the elaeagnus conferta fruits that 1kg soaks, it is smashed with machinery;

C. take by weighing pectase 2g, be dissolved in the 5kg water, filter, the preparation enzymolysis liquid, subsequent use;

D. the enzymolysis liquid with above-mentioned preparation adds in the mechanical elaeagnus conferta fruits of smashing, and filters behind 50 ℃ ± 5 ℃ hot reflux 2h, collects filtrating;

E. the pure water that adds 3kg again filters behind 80 ℃ ± 5 ℃ hot reflux 1h, collects filtrating;

F. the filtrating with d step and e step merges, and the decompression concentrated filtrate obtains the elaeagnus conferta fruits tincture.

Through area normalization method, try to achieve the relative percentage composition of each volatile ingredient with quantitative analysis respectively.The result is following:

Analysis result shows: elaeagnus conferta fruits tincture alcohols accounts for 12.19%, and aldehydes accounts for 26.08%, and saturated hydrocarbon compound accounts for 3.31%, and the ester class accounts for 3.08%.Detecting of this tincture contains a large amount of 5 hydroxymethyl furfurals, mysoinositol and a spot of ISDN in the thing, these make the effect of elaeagnus conferta fruits and effect more remarkable.Wherein 5 hydroxymethyl furfural have anti-oxidant, improve the effects favourable such as blood flow to human body, ISDN mainly is applicable to the treatment of angina pectoris and congestive heart failure.Other different composition all has effect in various degree.

The elaeagnus conferta fruits extract (tincture) that above-mentioned steps of the present invention obtains is the application of ratio in 100g cigarette composition leaf group of 0.1-0.3% by weight percentage.

The invention has the advantages that: have the fragrance of lifting concentration, rich, suppress assorted gas, the effect of stimulate, pleasant impression slightly improving.

The specific embodiment:

The extraction step of elaeagnus conferta fruits extract is with the description of summary of the invention part in the embodiment of the invention, and used instrument is the operation while prior art of market purchase, instrument among the embodiment.

Embodiment 1:

The application of elaeagnus conferta fruits extract in cigarette composition

Method for making sample: take by weighing 3 parts of cigarette composition leaf groups that 100g is identical; With the preparation after elaeagnus conferta fruits extract (tincture) by weight percentage 0.1%, 0.2%, 0.3% ratio add in the 100g cigarette composition leaf group; Be mixed evenly; In the airtight climatic chamber that is stored in 22 ℃ ± 2 ℃ of temperature and relative humidity 60% ± 5%, process the identical cigarette of auxiliary material specification behind 48 h and prop up, numbering as follows:

Sample experiment table

Carry out sensory evaluation according to GB5606.4-1996 " specification requirement of cigarette sense organ ", and its project is carried out qualitative description targetedly.Through sample is smoked panel test, the result is following:

The cigarette aesthetic quality table of smokeing panel test

Smell fragrant aspect, sample 1#, 2#, 3# compare with contrast 0#: it is fragrant with acid to send light fruital thoroughly, and with the fragrant harmony of tobacco, along with being increased in of addition has increase slightly on the intensity.Sensory evaluating smoking result shows: sample 1#, 2#, 3# compare with contrast 0#, and the sour rhythm in the fragrance increases, and has light fruital note; Fragrance is rich to have lifting with flue gas concentration; Assorted gas and excitant be little to have a reduction, but along with the increase of addition, fragrance passes through a property has reduction slightly; Nasal cavity stimulates has increase slightly, also can bring residual on the pleasant impression slightly.

Claims (2)

1. elaeagnus conferta fruits extract is characterized in that the elaeagnus conferta fruits extract obtains through following steps:

A. the elaeagnus conferta fruits that will just pluck is immersed in the ratio of 1:5 in 95% the ethanol, and is subsequent use;

B. take by weighing the elaeagnus conferta fruits that 1kg soaks, it is smashed with machinery;

C. take by weighing pectase 2g, be dissolved in the 5kg water, filter, the preparation enzymolysis liquid, subsequent use;

D. the enzymolysis liquid with above-mentioned preparation adds in the mechanical elaeagnus conferta fruits of smashing, and filters behind 50 ℃ ± 5 ℃ hot reflux 2h, collects filtrating;

E. the pure water that adds 3kg again filters behind 80 ℃ ± 5 ℃ hot reflux 1h, collects filtrating;

F. the filtrating with d step and e step merges, and the decompression concentrated filtrate obtains the elaeagnus conferta fruits tincture.

2. the described elaeagnus conferta fruits tincture of claim 1 application of ratio in 100g cigarette composition leaf group of 0.1-0.3% by weight percentage.