CN102367281B - Preparation method and application of ethanol extract of laminarin - Google Patents
Preparation method and application of ethanol extract of laminarin Download PDFInfo
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- CN102367281B CN102367281B CN 201110321907 CN201110321907A CN102367281B CN 102367281 B CN102367281 B CN 102367281B CN 201110321907 CN201110321907 CN 201110321907 CN 201110321907 A CN201110321907 A CN 201110321907A CN 102367281 B CN102367281 B CN 102367281B
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Abstract
The invention discloses a preparation method and an application of an ethanol extract of laminarin. The ethanol extract of laminarin is obtained through the preparation method which comprises the following steps: 1, crushing sea tangle, adding an ethanol solution to the crushed sea tangle, extracting for 2-5h at 50-80DEG C; 2, filtering to remove a residue, and adding active carbon to an extract to decolor and remove a fishy smell; and 3, filtering, and concentrating to obtain the ethanol extract of laminarin. The ethanol extract of laminarin is added to cut tobaccos or filter tips in a sprinkling mode, and the mass of the ethanol extract of laminarin accounts for 0.01-1.0% of the mass of cut tobaccos, so the content of free radicals in a mainstream smoke of cigarettes is effectively reduced, the reduction amplitude of the content of the free radicals is more than 15%, and the smoking quality of the cigarettes is improved, thereby the ethanol extract of laminarin, which has irritation reduction effects on nasal cavities, throats and the like, allows the smoke to be smooth, the taste to be soft, the fragrance to be kept, and no side effects to be possessed. The ethanol extract of laminarin has the advantages of no obviously fishy smell, easy obtaining, and low cost.
Description
Technical field
The present invention relates to marine plant extracts active ingredients field, be specifically related to a kind of preparation method and application thereof of laminarin ethanol extract.
Background technology
Epidemiology survey shows that smoking can cause human multiple disease, and as lung cancer, bronchitis, pulmonary emphysema, oral carcinoma, bladder cancer etc., and free radical is one of major reason that gives rise to diseases.Flue gas can produce alkoxy free group RO, alkyl diradical R, quinone/semiquinone free radical, polycyclic aromatic hydrocarbons free radical etc. in the smoking process, and directly or indirectly the attack cells composition causes certain damage to body.Along with smoking and health problem more and more receive publicity, the free-radical scavengers that reduces free-radical contents in the cigarette smoke and become one of the emphasis research topic development and utilization high effect nontoxic of cigarette harm reduction is the inevitable development trend of cigarette products.Zhengzhou Tobacco Research Institute of CNTC (application number 200610128282.9) discloses a kind of Hijiki polysaccharide and the application in tobacco thereof, (application number 200610128283.3) sargassum fusiforme aqueous is proposed extracting solution and the application in tobacco thereof, and (application number 200610128284.8) Sargassum fusiforme alcohol extract and the application in tobacco thereof, but it is less that Sargassum fusiforme gets kind, its frond g and D obviously is subjected to such environmental effects, therefore output is less, the extraction yield of Hijiki polysaccharide is not high simultaneously, and there is certain difficulty in its application.
Sea-tangle (Laminaria japonica) is a kind of marine plant with higher edible and pharmaceutical use, contains multiple active substance to the human body beneficial, has " hat of basic food " laudatory title.Laminarin is one of activeconstituents that extracts from sea-tangle, because China's sea-tangle resource is very abundant, so the research of laminarin is subjected to people's common concern.Studies show that, laminarin have remove that free radical is anti-oxidant, radioprotective, reducing blood-fat, hypoglycemic, regulate health-care effect such as immunologic function.At present, the laminarin more application is in medicinal use, and the application patent in foodstuffs industry is less, discloses a method for preparing kelp polysaccharide food as Fujian Province's aquatic products research (application number 200810071528.2); Dalian Polytechnic University (application number 200710157574.X) discloses a kind of preparation method of laminarin.Though laminarin has good biological activity,, do not have the report that a kind of laminarin ethanol extract is applied to the tobacco field at present.
Summary of the invention
The objective of the invention is to fill up at present about using the technological gap that the laminarin ethanol extract improves smoking property of cigarette quality aspect, a kind of laminarin ethanol extract is provided, described laminarin ethanol extract adds in the cigarette, can obviously improve the smoking property of cigarette quality, flue gas raises up, and sweet sense is arranged, and mouthfeel softens, reduction has positive effect to the stimulation of nasal cavity, throat etc.
Another object of the present invention is to provide the application in tobacco of described laminarin ethanol extract.
Above-mentioned purpose of the present invention is achieved by following technical solution:
A kind of preparation method of laminarin ethanol extract comprises the steps:
(1) sea-tangle adds ethanol after pulverizing, and extracts 2～5 hours down for 50～80 ℃ in oil bath;
(2) remove by filter residue, in extracting solution, add gac;
(3) filter, concentrate, obtain described laminarin ethanol extract.
Described ethanol is a kind of organic solvent commonly used, and as a kind of preferred version, described ethanol is 10%～80% aqueous ethanolic solution for ethanol weight.
As a kind of preferred version, in the step (1), described sea-tangle is pulverized to be preferably and is crushed to 10～100 orders.
As a kind of preferred version, in the step (1), described consumption of ethanol is 20～40 times of sea-tangle weight.
The laminarin ethanol extract that is obtained by above-mentioned preparation method.
The application of described laminarin ethanolic soln in tobacco is according to 0.01～1.0% of tobacco quality, by spray pattern laminarin solution added in pipe tobacco or the filter tip.
In the step (2), the described decolouring that act as that adds gac in the extracting solution, in addition, because sea-food generally all can have fishy smell, the sea-tangle ethanol extract that has fishy smell is being used for cigarette products, can bring assorted flavor to cigarette, influence mouthfeel, the contriver finds, gac in step (2), in decolouring, can also remove the fishy smell of product, thereby resulting sea-tangle ethanol extract can be directly applied in the cigarette products.
The addition of described gac is preferably 1～2 weight % of extracting solution.
As a kind of preferred version, the addition of described laminarin ethanolic soln is preferably 0.1% of tobacco quality.Described laminarin ethanolic soln joins on the pipe tobacco or filter tip of cigarette, can effectively reduce the cigarette mainstream flue gas free-radical contents, the free radical fall is more than 15%, also can improve the smoking property of cigarette quality, have reduction to hormesiss such as nasal cavity, throats, the flue gas cunning that attenuates, mouthfeel softens, and maintenance fragrance, without any side effects, improve the quality of tobacco product.
Compared with prior art, the present invention has following beneficial effect:
Laminarin ethanolic soln of the present invention is added in cigarette shreds or the filter tip, can effectively reduce the cigarette mainstream flue gas free-radical contents, also can improve the smoking property of cigarette quality, has reduction to hormesiss such as nasal cavity, throats, the flue gas cunning that attenuates, mouthfeel softens, and keeps fragrance, without any side effects, improve the quality of tobacco product.
Embodiment
Further explain the present invention below in conjunction with specific embodiment, but embodiment does not do any type of restriction to the present invention.
Embodiment 1
(1) sea-tangle is ground into 10 purpose sea-tangle powder after, get 50g and place container, mix with 1: 20 weight ratio with 50 weight % ethanolic solns, extracted 3 hours down 50 ℃ of oil baths;
(2) remove by filter residue, in extracting solution, add 1 weight % gac, handled 1 hour at 50 ℃;
(3) remove by filter gac, being concentrated into dry matter content is 5%～10%, obtains described laminarin ethanol extract.Products obtained therefrom is numbered laminarin ethanol extract 1#.
The IC50 of laminarin ethanol extract 1# in the DPPH free radical system is 0.48mg/mL.
Embodiment 2
(1) sea-tangle is ground into 40 purpose sea-tangle powder after, get 50g and place container, mix with 1: 25 weight ratio with 60 weight % ethanolic solns, extracted 4 hours down 70 ℃ of oil baths;
(2) remove by filter residue, in extracting solution, add 2 weight % gacs, handled 1 hour at 55 ℃;
(3) remove by filter gac, being concentrated into dry matter content is 5%～10%, obtains described laminarin ethanol extract.Products obtained therefrom is numbered laminarin ethanol extract 2#.
The IC50 of laminarin ethanol extract 2# in the DPPH free radical system is 0.24mg/mL.
Embodiment 3
(1) sea-tangle is ground into 60 purpose sea-tangle powder after, get 50g and place container, mix with 1: 30 weight ratio with 70 weight % ethanolic solns, extracted 4 hours down 70 ℃ of oil baths;
(2) remove by filter residue, in extracting solution, add 1 weight % gac, handled 1 hour at 60 ℃;
(3) remove by filter gac, being concentrated into dry matter content is 5%～10%, obtains described laminarin ethanol extract.Products obtained therefrom is numbered laminarin ethanol extract 3#.
The IC50 of laminarin ethanol extract 3# in the DPPH free radical system is 0.30mg/mL.
Embodiment 4
(1) sea-tangle is ground into 80 purpose sea-tangle powder after, get 50g and place container, mix with 1: 40 weight ratio with 80 weight % ethanolic solns, extracted 3 hours down 60 ℃ of oil baths;
(2) remove by filter residue, in extracting solution, add 2 weight % gacs, handled 1 hour at 60 ℃;
(3) remove by filter gac, being concentrated into dry matter content is 5%～10%, obtains described laminarin ethanol extract.Products obtained therefrom is numbered laminarin ethanol extract 4#.
The IC50 of laminarin ethanol extract 3# in the DPPH free radical system is 0.32mg/mL.
Anti-oxidant value and the applicating evaluating of embodiment 5 laminarin products
Embodiment 1～4 gained laminarin is by 0.03%, 0.05%, 0.1% mixing with pipe tobacco of accounting for tobacco quality, detect the test laminarin to the removing effect of cigarette mainstream flue gas free-radical contents with flue gas collector and spectrum spectral method (ESR), as shown in table 1:
The different additions of table 1 product are to the removing effect of cigarette mainstream flue gas free-radical contents
Annotate :+, free-radical contents raises;-, free-radical contents reduces
Above-mentioned laminarin ethanol extract 1#～4# is sprayed onto in the pipe tobacco by certain addition, carries out the sensory evaluating smoking then, the result is as shown in table 2.
The evaluation and analysis result that table 2 product is used in cigarette
By the evaluation and analysis result as can be known: laminarin ethanol extract of the present invention is applied in the cigarette, the cigarette quality be improved significantly, the lubricated sense in oral cavity promotes, mouthfeel is better.
Claims (3)
1. the application of laminarin ethanol extract in tobacco is characterized in that, according to 0.01～1.0% of tobacco quality, by spray pattern the laminarin ethanol extract added in pipe tobacco or the filter tip;
The preparation method of described laminarin ethanol extract comprises the steps:
(1) sea-tangle adds ethanol after pulverizing, and extracts 2～5 hours down for 50～80 ℃ in oil bath;
(2) remove by filter residue, in extracting solution, add gac;
(3) filter, concentrate, obtain described laminarin ethanol extract;
Wherein, in the step (1), described sea-tangle is pulverized to being crushed to 10～100 orders;
In the step (1), described ethanol is 10%～80% aqueous ethanolic solution for ethanol weight;
The addition of the described gac of step (2) is 1～2 weight % of extracting solution.
2. application according to claim 1 is characterized in that, in the step (1), described consumption of ethanol is 20～40 times of sea-tangle weight.
3. application according to claim 1 is characterized in that, the preparation method of described laminarin ethanol extract comprises the steps:
(1) sea-tangle is ground into 40 purpose sea-tangle powder after, get 50g and place container, mix with the 1:25 weight ratio with 60 weight % ethanolic solns, extracted 4 hours down 70 ℃ of oil baths;
(2) remove by filter residue, in extracting solution, add 2 weight % gacs, handled 1 hour at 55 ℃;
(3) remove by filter gac, being concentrated into dry matter content is 5% ~ 10%, obtains described laminarin ethanol extract.
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| CN 201110321907 CN102367281B (en) | 2011-10-20 | 2011-10-20 | Preparation method and application of ethanol extract of laminarin |
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| CN 201110321907 CN102367281B (en) | 2011-10-20 | 2011-10-20 | Preparation method and application of ethanol extract of laminarin |
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| CN102367281B true CN102367281B (en) | 2013-07-17 |
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| CN102599650A (en) * | 2012-04-06 | 2012-07-25 | 贵州省烟草科学研究所 | Health-care cigarettes |
| CN103040087B (en) * | 2012-11-07 | 2015-01-07 | 广东中烟工业有限责任公司 | Preparation method and application of functionality tobacco sheets for improving shred conglutination and sweet returning |
| CN103005710A (en) * | 2012-12-27 | 2013-04-03 | 广东中烟工业有限责任公司 | Low-hazard cigarette filter stick and preparation method thereof, and low-hazard cigarette |
| CN106666501A (en) * | 2016-12-29 | 2017-05-17 | 湖州师范学院 | Preparation method and application of instant laver powder capable of regulating intestinal probiotics |
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| CN1069523C (en) * | 1998-10-14 | 2001-08-15 | 中国科学院海洋研究所 | Application of alginic polysaccharide sulfate in preparation of medicine for treating renal failure |
| CN1875795B (en) * | 2006-07-03 | 2010-04-14 | 红云烟草(集团)有限责任公司 | Algae compound addictive for cigarette and preparation method thereof |
| CN1962699B (en) * | 2006-11-25 | 2012-02-01 | 中国烟草总公司郑州烟草研究院 | Hijiki polysaccharide and its uses in tobacco |
| CN101036698B (en) * | 2007-04-17 | 2010-05-19 | 广东中烟工业有限责任公司 | Mulberry leaf extract and its preparing process and application |
| CN100464756C (en) * | 2007-04-27 | 2009-03-04 | 山东大学 | Application of fucoidan in the preparation of medicine and health-care products for preventing and treating neurodegenerative diseases |
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| CN101928353B (en) * | 2010-08-13 | 2012-07-18 | 内蒙古昆明卷烟有限责任公司 | Extraction of cordyceps polysaccharide and application of cordyceps polysaccharide in cigarettes |
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