CN102668961B - Early evaluation method for salt tolerance of willow coastal mud flat - Google Patents
Early evaluation method for salt tolerance of willow coastal mud flat Download PDFInfo
- Publication number
- CN102668961B CN102668961B CN2012101604226A CN201210160422A CN102668961B CN 102668961 B CN102668961 B CN 102668961B CN 2012101604226 A CN2012101604226 A CN 2012101604226A CN 201210160422 A CN201210160422 A CN 201210160422A CN 102668961 B CN102668961 B CN 102668961B
- Authority
- CN
- China
- Prior art keywords
- willow
- cut
- solution
- illumination
- spray
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses an early evaluation method for the salt tolerance of the willow coastal mud flat. The method comprises the following steps of: taking a one-year old twig from the limb; diluting sea water into solutions with different mass concentrations of salt content; pouring the solutions with different concentrations into a black plastic water tank respectively; making a cottage plate; inserting the twig into the cottage plate; and culturing the water tank in an illumination culture box. The method disclosed by the invention is easy to master and convenient and quick and basically not limited by external environment, and can be implemented all year round; the evaluation speed is high, and generally the evaluation result can be obtained in one week; and moreover, the method requires few experimental materials, has small error and high efficiency, and is widely applicable.
Description
Technical field
The present invention relates to the early stage authentication method of the coastal beach salt resistance of a kind of willow.
Background technology
Willow, Salicaceae (Salicaceae) Salix, deciduous tree or shrub, rare volt undershrub of crawling.Sprig does not have terminal bud, 1 piece of perula.Single leaf, lanceolar or ovum shape lanceolar, leaf margin has sawtooth, rare full edge, petiole is short.Flower unisexuality, dioecism, stamen 2,3,5 or most, filigree base portion tool body of gland.Female flower does not have perianth, capsule, and 2 split.Seed is little, hairiness, and plant has certain salt resistance ability.
The salt resistance of forest is the interactional a kind of general performances of many proterties, also unifies perfect index identification systems at present both at home and abroad, and different forests have different salt resistance abilities because its salt tolerant mode is different with Mechanism of Salt-tolerant.The tradition authentication method is directly to pass through relatively its survival rate, amount of growth on the basis of saline land plantation, bright dry weight is recently identified, because the time long, that estimate needs such as its growth traits, phenology proterties, vegetative period tree growth phase is grown, wastes time and energy, error is big, efficient is low and be subjected to outer border environmental disturbances, accurate rate variance.
Summary of the invention
The object of the present invention is to provide a kind of efficient height, the early stage authentication method of the coastal beach salt resistance of willow accurately.
Technical solution of the present invention is:
The early stage authentication method of the coastal beach salt resistance of a kind of willow is characterized in that: comprise the following steps:
(1) from the annual spray of limb intercepting, be cut into 8-10cm length, end opening is tiltedly cut 45 degree at the minor matters place, and to enlarge contact area, straight snips suitable for reading only stays two leaves on each spray, and blade is all cut off half;
(2) adopt illumination box, set up illumination in 12 hours, 12 hours unglazed photoenvironments, intensity of illumination is 3300Lux, and temperature is set at 25 ℃, adopts the black cloth shading on the incubator glass door, to reduce experimental error;
(3) get the solution that seawater is diluted to 1 ‰, 2 ‰, 3 ‰, 4 ‰, 5 ‰, 6 ‰ different quality concentration salt content, and the solution of above-mentioned variable concentrations is poured into respectively in the black plastic tank;
(4) the cuttage plate is made: the thick polystyrene plates of 2cm is punched, and aperture 0.7-0.8cm is made into the cuttage plate;
(5) will insert the cuttage plate through the spray that step (1) is handled, 3cm is reserved to immerse in the sea water solution in the lower end, and the cuttage plate is cut into black plastic tank internal diameter size to guarantee to take root dark surrounds;
(6) tank is put into illumination box and cultivate, took out observation on the 5th day, the first water concentration solution that the willow kind is normally taken root is the salt resistance that this kind has this mass concentration.
The present invention compares with conventional method and has following advantage:
(1) method is easily grasped, and is not subjected to the restriction of external environment substantially, and is convenient and swift, and the anniversary can carry out;
(2) evaluation speed is fast, just can identify the result in the general week;
(3) required experiment material is few, and error is little, and the efficient height is widely used.
The present invention can identify the coastal beach salt resistance of each veriety of willow rapidly.
The invention will be further described below in conjunction with embodiment.
Embodiment
The early stage authentication method of the coastal beach salt resistance of a kind of willow comprises the following steps:
(1) from the annual spray of limb intercepting, be cut into 8-10cm length, end opening is tiltedly cut 45 degree at the minor matters place, and to enlarge contact area, straight snips suitable for reading only stays two leaves on each spray, and blade is all cut off half;
(2) adopt illumination box, set up illumination in 12 hours, 12 hours unglazed photoenvironments, intensity of illumination is 3300Lux, and temperature is set at 25 ℃, adopts the black cloth shading on the incubator glass door, to reduce experimental error;
(3) get the solution that seawater is diluted to 1 ‰, 2 ‰, 3 ‰, 4 ‰, 5 ‰, 6 ‰ different quality concentration salt content, and the solution of above-mentioned variable concentrations is poured into respectively in the black plastic tank;
(4) the cuttage plate is made: the thick polystyrene plates of 2cm is punched, and aperture 0.7-0.8cm is made into the cuttage plate;
(5) will insert the cuttage plate through the spray that step (1) is handled, 3cm is reserved to immerse in the sea water solution in the lower end, and the cuttage plate is cut into black plastic tank internal diameter size to guarantee to take root dark surrounds;
(6) tank is put into illumination box and cultivate, took out observation on the 5th day, the first water concentration solution that the willow kind is normally taken root is the salt resistance that this kind has this mass concentration.
Claims (1)
1. the early stage authentication method of the coastal beach salt resistance of willow is characterized in that: comprise the following steps:
(1) from the annual spray of limb intercepting, be cut into 8-10cm length, end opening is tiltedly cut 45 degree at the minor matters place, and to enlarge contact area, straight snips suitable for reading only stays two leaves on each spray, and blade is all cut off half;
(2) adopt illumination box, set up illumination in 12 hours, 12 hours unglazed photoenvironments, intensity of illumination is 3300Lux, and temperature is set at 25 ℃, adopts the black cloth shading on the incubator glass door, to reduce experimental error;
(3) get the solution that seawater is diluted to 1 ‰, 2 ‰, 3 ‰, 4 ‰, 5 ‰, 6 ‰ different quality concentration salt content, and the solution of above-mentioned variable concentrations is poured into respectively in the black plastic tank;
(4) the cuttage plate is made: the thick polystyrene plates of 2cm is punched, and aperture 0.7-0.8cm is made into the cuttage plate;
(5) will insert the cuttage plate through the spray that step (1) is handled, 3cm is reserved to immerse in the sea water solution in the lower end, and the cuttage plate is cut into black plastic tank internal diameter size to guarantee to take root dark surrounds;
(6) tank is put into illumination box and cultivate, took out observation on the 5th day, the first water concentration solution that the willow kind is normally taken root is the salt resistance that this kind has this mass concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101604226A CN102668961B (en) | 2012-05-23 | 2012-05-23 | Early evaluation method for salt tolerance of willow coastal mud flat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101604226A CN102668961B (en) | 2012-05-23 | 2012-05-23 | Early evaluation method for salt tolerance of willow coastal mud flat |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102668961A CN102668961A (en) | 2012-09-19 |
| CN102668961B true CN102668961B (en) | 2013-08-21 |
Family
ID=46802056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101604226A Expired - Fee Related CN102668961B (en) | 2012-05-23 | 2012-05-23 | Early evaluation method for salt tolerance of willow coastal mud flat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102668961B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972230A (en) * | 2012-12-17 | 2013-03-20 | 张健 | Salt pond for experiment of plant seedlings |
| CN105746317B (en) * | 2016-02-07 | 2019-01-01 | 浙江省农业科学院 | A kind of early screening method of resistance to low calcium cabbage strain |
| CN108651273B (en) * | 2018-05-17 | 2021-06-15 | 南通大学 | Method for breeding salt-tolerant lagerstroemia indica leaves by polymerization based on gene detection |
| CN109348952B (en) * | 2018-10-17 | 2020-08-25 | 山东省林业科学研究院 | Method for improving salt tolerance of salix matsudana |
| CN109609672A (en) * | 2018-11-13 | 2019-04-12 | 江苏沿江地区农业科学研究所 | The molecular labeling of willow salt-tolerance character main effect QTL and application |
| CN110476635A (en) * | 2019-09-03 | 2019-11-22 | 南通大学 | The sticking device and its screening technique of forest screening salt-enduring cultivars difficult to take root |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09266726A (en) * | 1996-03-29 | 1997-10-14 | Hitachi Ltd | Evaluation of salt resistance of plant |
| CN101294943B (en) * | 2008-06-05 | 2011-08-31 | 上海交通大学 | Device and brine solution for fast appraising salinity and alkalinity resistance of tree |
| CN102187774A (en) * | 2010-03-15 | 2011-09-21 | 西北农林科技大学 | System for identifying and evaluating seedling resistance of malus |
-
2012
- 2012-05-23 CN CN2012101604226A patent/CN102668961B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102668961A (en) | 2012-09-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102668961B (en) | Early evaluation method for salt tolerance of willow coastal mud flat | |
| CN103843593B (en) | A kind of cucumber grafting seedling-cultivating method | |
| CN107466686B (en) | Camellia oleifera Abel chopping and cleft grafting cultivation technology | |
| CN104067860B (en) | A kind of method of Chinese scholar tree grafting gold branch Chinese scholartree | |
| CN103039251B (en) | Cutting propagation method of leaf-borne jasmine twig | |
| CN106962028A (en) | Camellia cuttage grafting integration quick-breeding method | |
| CN103278367A (en) | Fast diagnosis method for liberobacter asiaticum | |
| CN104798564A (en) | Rose cuttage reproduction method taking flower mud as matrix | |
| CN104081958A (en) | Current year cuttage and flower growing for bougainvillea speetabilis | |
| CN105766409B (en) | A kind of method that saccharose treatment promotes chrysanthemum to bloom | |
| CN104025853A (en) | Cutting seedling raising method adopting partition board to block roots of cedar | |
| CN105532331A (en) | Garden anti-exposure sunshade net | |
| CN108901576A (en) | Ginkgo seedling multiple spot is abutted against the method and its fast big tree obtained and application of fast big tree | |
| CN102841006A (en) | Preparation method of plant tissue transmission electron microscope sample | |
| CN104737832A (en) | Apple tree grafting method | |
| Qi et al. | Photosynthetic characteristic of an endangered species Camellia nitidissima and its conservation implications | |
| CN101755593A (en) | Method for dividing and breeding Calathea veitchiana | |
| CN103225330B (en) | Method for getting water from air through phase change caused by light and using water for cultivation | |
| CN203136665U (en) | Flue-cured tobacco stereoscopic-shielding cultivation shield | |
| Tang | Distribution of C 4 plants along the Northeast China transect and its correlation to the environmental factors | |
| Ignatov et al. | Ephemerum spinulosum Bruch & Schimp.(Bryophyta), a new species for Russia | |
| CN102313653B (en) | Sampling method of desert biological crust soil ground sheets | |
| CN104663393A (en) | Hydroponic cultivation method for dracaena sanderiana | |
| CN204466335U (en) | The high-order side grafting system in moulding tasselled grafting day fragrant osmanthus | |
| CN106171536A (en) | Grafting true cypress technology on a kind of cypress |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130821 |