CN102660512B - Enzyme preparation composition for paddy rice and use thereof - Google Patents
Enzyme preparation composition for paddy rice and use thereof Download PDFInfo
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- CN102660512B CN102660512B CN 201210113355 CN201210113355A CN102660512B CN 102660512 B CN102660512 B CN 102660512B CN 201210113355 CN201210113355 CN 201210113355 CN 201210113355 A CN201210113355 A CN 201210113355A CN 102660512 B CN102660512 B CN 102660512B
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Abstract
The invention discloses an enzyme preparation composition for paddy rice and a use thereof, and belongs to the field of agricultural additives. The enzyme preparation composition for paddy rice comprises 45 to 50% of a peroxidase, 15 to 20% of a neutral protease, 15 to 20% of an amylase and 10 to 25% of cellulose. The enzyme preparation composition for paddy rice has the advantages that use is convenient; a plantation process is avoided; a water loss of seedling roots is avoided in seed germination; a percentage establishment of paddy rice seedlings is greatly improved; and a paddy rice yield increasing effect reaches to a significant level.
Description
Technical field
The invention discloses a kind of zymin composition and application thereof for paddy rice, belong to agricultural additive technology field.
Background technology
In plant growing process, the fertilizer of use mainly is chemical fertilizer such as nitrogenous fertilizer, phosphate fertilizer, potash fertilizer.Apply the component that too much chemical fertilizer can cause soil and change, cause the objectionable constituent in soil compaction, the soil to increase, and the lavishment chemical fertilizer also is the arch-criminal who causes ecological problems such as body eutrophication.Along with development of biology, the expert of various countries making great efforts research with biological method of improving the soil, has had multiple microbial fertilizer to come out now.But the effect of microbial fertilizer is subjected to the influence of weather and edatope very big, the easy inactivation of storage process, and effectively bacterial classification is single, and suitability is not very wide, so the investigator in this field strengthens the effect of bio-feritlizer and widens its use range trying every possible means.In recent years, the emergence of fermentation using enzyme industry, make biological vital movement important factor---the kind of enzyme day by day increases, cost also reduces greatly, making various zymins be applied to industrial and agricultural production becomes possibility.
It is still rare that zymin is used for the paddy rice plantation, CN101434498A discloses a kind of farm crop nourishing complex enzyme and application thereof, it comprises proteolytic enzyme, phytase, amylase, cellulase, polygalacturonase etc., be plurality of enzymes preparation compound prescription, by decomposing the macromolecular substance in the soil, can improve the soil, the nutritive substance that provides for the crop growth equilibrium, make the farm crop stalwartness, be conducive to growth and the maturation of farm crop, thereby improve the output of farm crop.Its main technique is that raw materials such as proteolytic enzyme, phytase, amylase, cellulase, polygalacturonase are mixed the composite the finished product that get.This invention is decomposed macromolecular substance in the soil by give the crop seeds afterbirth with zymin, for crop growth provides nutritive substance, this product deficiency: afterbirth may influence seed germination rate, use may destroy the original afterbirth of seed, and the solid powdery product is not easy to carry out the afterbirth operation.
Summary of the invention
The objective of the invention is to overcome the deficiency of currently available products function and the drawback of utilisation technology, a kind of zymin composition and application method thereof that can improve paddy rice adult seedling rate and output is provided.
For achieving the above object, a kind of zymin composition of the present invention is made up of following component by weight percentage:
Peroxidase: 45～50%;
Neutral protease: 15～20%;
Amylase: 15～20%;
Cellulase: 10～25%;
Described preparation of compositions method is: with described peroxidase, neutral protease, amylase, cellulase respectively through the degerming Plate Filtration, suck mixing in the automated fluid stirred pot according to above-mentioned per-cent, through micro-filtrate membrane filtration, detect qualified after, quantitative filling.
Among the present invention, the preferred catalase of described peroxidase.
Among the present invention, warm α-Dian Fenmei during described amylase is preferred.
Among the present invention, the aperture of described microfiltration membrane is 0.1～0.8 μ m.
Among the present invention, described zymin composition is applied to the seed soaking process of paddy rice.
When the present invention is used for the seed soaking process of paddy rice, be the last seed soaking that described prozyme is used for accelerating germination of rice seed, with this prozyme aqueous solution seed soaking 3-6h of massfraction 0.5～2%.
The present invention is by the research to vital movements such as rice paddy seed sprouting and rice plant growths, several enzymes closely bound up with the whole vital process of paddy rice have been found out, be peroxidase, amylase, neutral protease and cellulase, with after these enzyme degerming according to research the composite compound enzymic preparation with corresponding function that produces of proportioning.When paddy rice vernalization, soak seed with this product, can accelerate the decomposition of protein in the seed, starch homenergic material, for seed germination better provides the energy, participate in Conversion of energy and the substance metabolism process of paddy growth directly, accelerate energy accumulation, thereby reach the germinating time that shortens seed, improve rate of emergence and adult seedling rate, finally improve the effect of rice yield.
Embodiment
Be further described below in conjunction with the present invention of embodiment, but protection scope of the present invention not only is confined to embodiment.
Embodiment 1
Each component respectively through the degerming Plate Filtration, is sucked mixing in the automated fluid stirred pot according to the weight percent of embodiment in the table 11, the micro-filtrate membrane filtration through 0.1 μ m aperture, detect qualified after, quantitative filling.
When paddy rice vernalization is soaked seed for the last time, be 0.5% prozyme aqueous solution seed soaking 6 hours with concentration, normally sow seedling then.
Embodiment 2:
With each component respectively through the degerming Plate Filtration, suck in the automated fluid stirred pot according to the weight percent of embodiment in the table 12 composite, the micro-filtrate membrane filtration through 0.3 μ m aperture, detect qualified after, quantitative filling.
When paddy rice vernalization is soaked seed for the last time, be 1% prozyme aqueous solution seed soaking 4 hours with concentration, normally sow seedling then.
Embodiment 3:
With each component respectively through the degerming Plate Filtration, suck in the automated fluid stirred pot according to the weight percent of embodiment in the table 13 composite, the micro-filtrate membrane filtration through 0.8 μ m aperture, detect qualified after, quantitative filling.
When paddy rice vernalization is soaked seed for the last time, be 2% prozyme aqueous solution seed soaking 3 hours with concentration, normally sow seedling then.
The weight percent of various dhdps enzymes among the different embodiment of table 1
Experiment effect of the present invention:
1 materials and methods
1) test soil situation: sample plot soil is burgundy, the soil agrochemistry proterties: organic 14.2gkg
-1Full nitrogen 3.13gkg
-1, full phosphorus 1.27gkg
-1, full potassium 15.1gkg
-1, alkali-hydrolyzable nitrogen 227.6mgkg
-1, rapid available phosphorus 17.3mgkg
-1, available potassium 253.1mgkg
-1, pH value 7.17.
2) test materials: rice varieties is lucky round-grained rice 60.
3) test method: 2 processing are established in test.
Handle 1: control group (CK), adult seedling rate is calculated in normal vernalization, fertilising, sowing after the seedling, and output is calculated in ripe back.
Handle 2: test group, when vernalization is soaked seed for the last time, replace the seed soaking water with the prozyme product aqueous solution of massfraction 1%, behind the seed soaking 4h, adult seedling rate is calculated in normal sowing, fertilising, sowing after the seedling, and output is calculated in ripe back.Its result is shown in table 2 and table 3.
Table 2 adds prozyme composition of the present invention to the influence of paddy rice adult seedling rate
Annotate: compare with control group,
*P＜0.01
Table 3 adds prozyme composition of the present invention to the influence of rice yield
Annotate: compare with control group,
*P＜0.01
2, test-results
Test-results shows (table 2,3), product of the present invention to rice seed soaking after, adult seedling rate is compared with blank, has improved 15.27%, output is compared with blank, has improved 12.5%, all reaches extremely significantly standard.Illustrate that the present invention has overcome the shortcoming of using prior art, has reached the purpose that improves paddy rice adult seedling rate and output.
It should be noted that at last: above embodiment is only in order to illustrate the present invention and unrestricted technical scheme described in the invention; Therefore, although this specification sheets has been described in detail with reference to above-mentioned the present invention of each embodiment,, those of ordinary skill in the art should be appreciated that still and can make amendment or be equal to replacement the present invention; And all do not break away from technical scheme and the improvement thereof of the spirit and scope of the present invention, and it all should be encompassed in the claim scope of the present invention.
Claims (5)
1. zymin composition that is used for paddy rice is characterized in that described composition is made up of following component by weight percentage:
Catalase: 45～50%;
Neutral protease: 15～20%;
Amylase: 15～20%;
Cellulase: 10～25%;
Described preparation of compositions method is: with described catalase, neutral protease, amylase, cellulase respectively through the degerming Plate Filtration, suck automated fluid stirred pot mixing according to above-mentioned per-cent, through micro-filtrate membrane filtration, detect qualified after, quantitative filling.
2. zymin composition according to claim 1 is characterized in that, described amylase is middle temperature α-Dian Fenmei.
3. zymin composition according to claim 1 is characterized in that, the aperture of described microfiltration membrane is 0.1～0.8 μ m.
4. the application of a zymin composition as claimed in claim 1 is characterized in that, described zymin composition is applied to the process of rice seed soaking.
5. the application of a zymin composition as claimed in claim 4, it is characterized in that, the application of described zymin composition in the rice seed soaking process is specially: when rice paddy seed is soaked seed for the last time in vernalization, use the aqueous solution seed soaking 3～6h of the zymin composition of massfraction 0.5～2%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210113355 CN102660512B (en) | 2012-04-17 | 2012-04-17 | Enzyme preparation composition for paddy rice and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210113355 CN102660512B (en) | 2012-04-17 | 2012-04-17 | Enzyme preparation composition for paddy rice and use thereof |
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| CN102660512A CN102660512A (en) | 2012-09-12 |
| CN102660512B true CN102660512B (en) | 2013-07-03 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106612732A (en) * | 2016-12-13 | 2017-05-10 | 韦克康 | Method for raising seedlings from radix bupleuri seeds |
| CN117730148A (en) * | 2021-07-27 | 2024-03-19 | 合同酒精株式会社 | Enzyme-containing composition, method for producing milk, and method for producing fermented milk |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110151056A1 (en) * | 1998-11-20 | 2011-06-23 | Uchenna Chukwu | Method of enzymatically degrading raw whole vegetables |
| CN101434498A (en) * | 2007-11-16 | 2009-05-20 | 郝伟星 | Corps nutrient complex enzyme and use thereof |
| CN101899423A (en) * | 2009-12-30 | 2010-12-01 | 魏海峰 | Special composite enzyme preparation for malting |
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