CN102657298B - Body color intensifier for large yellow croaker - Google Patents
Body color intensifier for large yellow croaker Download PDFInfo
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- CN102657298B CN102657298B CN201210178803.7A CN201210178803A CN102657298B CN 102657298 B CN102657298 B CN 102657298B CN 201210178803 A CN201210178803 A CN 201210178803A CN 102657298 B CN102657298 B CN 102657298B
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- large yellow
- yellow croaker
- body colour
- reinforcing agent
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Abstract
The invention relates to a body color intensifier for large yellow croakers. The body color intensifier is prepared from the following components in percentage by weight: 20-45wt% of astaxanthin, 20-45wt% of lutein and 20-45wt% of canthaxanthin. The body color intensifier disclosed by the invention is used as a feed additive used in the artificial compound feed for the large yellow croakers. The additive amount is only 0.01-0.15%, and the body color of the artificially cultured large yellow croakers can be obviously improved after the body color intensifier is used about 8 weeks. Meanwhile, the body color intensifier disclosed by the invention is used together with a large yellow croaker feed prepared by the applicant to obtain a preferable effect.
Description
Technical field
The invention belongs to feeding additive aquatic animal field, be specifically related to a kind of body colour reinforcing agent that can effectively improve cultured large yellow croaker body colour.
Technical background
Large yellow croaker is that the three large seawater that Chinese output is the highest are propagated one of fish artificially, and 2011 annual productions reach 80,000 tons.Wild large yellow croaker back and side present yellowish-brown, belly golden yellow, each fin yellow or lark, lip Chinese red.Owing to propagating artificially at present, in the bait that large yellow croaker used, lack large yellow croaker body colour and form carotenoid necessary and himself can not be synthetic, cause the large yellow croaker body colour serious degradation of propagating artificially, body colour is thin out to bleach, and has had a strong impact on quality and the market price of propagating large yellow croaker artificially.Little about the research report of large yellow croaker body colour at present, only limit to the research report of curcumin on large yellow croaker, but curcumin is a kind of dyestuff, the not necessary material of fish body colour self-assembling formation.Therefore industry is badly in need of providing a kind of and significantly improving the body colour reinforcing agent of propagating large yellow croaker body colour artificially.
Summary of the invention
The object of this invention is to provide a kind of large yellow croaker body colour reinforcing agent, can significantly improve the body colour of propagating large yellow croaker artificially, thereby make up the deficiencies in the prior art.
A kind of large yellow croaker body colour reinforcing agent, includes astaxanthin, lutein and canthaxanthin, and its mass percent composition is respectively: 20-45%, 20-45% and 20-45%.
Above-mentioned body colour reinforcing agent, the mass ratio that a kind of preferred group becomes astaxanthin, lutein and canthaxanthin is 1:1:1.
The application process of above-mentioned large yellow croaker body colour reinforcing agent, the addition mass percent in large yellow croaker feed is 0.01-0.15%.
Above-mentioned large yellow croaker feeding quality percentage is composed as follows: white fish meal 43%, dregs of beans 21%, wheat flour 20-21%, brewer's yeast 2%, fish oil 7.1%, soybean lecithin 1.5%, multidimensional 2%, many ore deposits 2%, phagostimulant 0.3%, ethoxyquinoline 0.1%, mould inhibitor 0.1%.
Phagostimulant is selected the mixture of glycine and betaine, and the mass ratio of glycine and betaine is 1:1.
Mould inhibitor is selected the mixture of calcium propionate and fumaric acid, and the mass ratio of calcium propionate and fumaric acid is 1:1.
Body colour reinforcing agent of the present invention is used in large yellow croaker artifical compound feed as feed addictive, and addition is only 0.01-0.15%, uses and within about 8 weeks, just can significantly improve the body colour of propagating large yellow croaker artificially.Meanwhile, when the large yellow croaker feed that body colour reinforcing agent of the present invention coordinates applicant to prepare is used, can obtain better effect.
Accompanying drawing explanation
Fig. 1: the large yellow croaker skin of abdomen brightness value (L that the present invention detects
*);
Fig. 2: the large yellow croaker skin of back brightness value (L that the present invention detects
*);
Fig. 3: the large yellow croaker skin of abdomen red value (a that the present invention detects
*);
Fig. 4: the large yellow croaker skin of back red value (a that the present invention detects
*);
Fig. 5: the yellow value of the large yellow croaker belly (b that the present invention detects
*);
Fig. 6: the large yellow croaker back yellow value (b that the present invention detects
*);
Fig. 7: the large yellow croaker skin of abdomen carotenoid content that the present invention detects;
Fig. 8: the large yellow croaker skin of back carotenoid content that the present invention detects.
The specific embodiment
Body colour reinforcing agent of the present invention is that astaxanthin, lutein and canthaxanthin is formulated according to applicant's definite proportioning that studies for a long period of time.Astaxanthin, lutein and canthaxanthin can be selected commercially available product, for example astaxanthin be DSM N. V. produce to add beautiful element pink, auroxanthin and intelligence spy that lutein and canthaxanthin select Guangzhou Zhiteqi Biotechnology Co., Ltd to produce are red.
Below in conjunction with specific embodiment, the component of body colour reinforcing agent of the present invention is described.
Embodiment 1:
Astaxanthin, lutein and canthaxanthin are mixed, and its mass percent is respectively 30%, 30% and 35%, also includes in addition the adhesive of bait.
Embodiment 2:
Astaxanthin, lutein and canthaxanthin are mixed, and its mass percent is respectively 20%, 40%, 40%.
Embodiment 3:
Astaxanthin, lutein and canthaxanthin are mixed, by the proportioning of 1:1:1, mix.
With the large yellow croaker feed that do not add body colour reinforcing agent of the present invention as a control group, the body colour reinforcing agent of the embodiment of the present invention 3 is added in the large yellow croaker feed the same with control group by the addition of 0.01-0.15%, using above-mentioned feed as experiment feed, carry out culture experiment.
Select the same a collection of large yellow croaker of artificial incubation then as experiment fish, average weight is 13.56 ± 0.05g.Pick out in consistent, the athletic large yellow croaker of the specification seawater floating cage that to put in a suitable place to breed in 6 specifications be 1m × 1m × 1.5m, in each net cage, put 60 tails in a suitable place to breed.3 net cages of every kind of feedstuff feeding, culture experiment carries out 8 weeks altogether.Culture experiment finishes carotenoid content in rear mensuration large yellow croaker body colour value and skin, and using wild large yellow croaker as reference.
Skin volume colour is measured: the L that adopts the international luminous lighting CIE of the committee (1976) regulation
*, a
*, b
*the colour space represents large yellow croaker skin body colour, wherein L
*represent brightness value ,-b
*represent blue valve ,+b
*represent yellow value ,-a
*represent green value ,+a
*represent red value.Use colour difference meter in the 8th week night 19:30～23:30 carry out body colour mensuration, colour difference meter uses front to be calibrated instrument with blank.Measure respectively the body colour value of large yellow croaker back and skin of abdomen, back is the region between dorsal fin leading edge below and side line top, and belly is that the abdomeinal fin of large yellow croaker belly is to the region between anal fin.The random mensuration of each net cage 4 tail fishes.
(2) mensuration of skin carotenoid content: experiment finishes that rear each net cage is got 5 tail fish dorsals and skin of abdomen is preserved in-20 ℃ of refrigerators, for measuring the carotenoid content of skin.Get 0.1000g(and be accurate to 0.0001g) skin in test tube, add 10ml ethyl acetate: ethanol (1:1, V/V) homogenate, pipettes supernatant after the centrifugal 5min of 4000g; Then with 5ml ethyl acetate and 10ml n-hexane, extract respectively once respectively, after finally three's supernatant being concentrated, with High Purity Nitrogen, dry up.Pigment after drying up is dissolved in 4ml chromatographically pure acetone (containing 0.05%BHT), mixes the centrifugal 5min of 10000g.At 448nm place, measure with honourable photometer.Carotenoid content computing formula=10000*V*A/W/E
1%, 1 cm, the volume that wherein V is extract, ml; A is absorbance; W is example weight, g; E
1%, 1 cmfor extinction coefficient.
Adopt SPSS15.0 to carry out variance analysis to data.When reaching remarkable, difference carries out multiple comparison analyse, level of signifiance P<0.05 with Tukey check.
Experimental result shows: as shown in Figure 1, and experimental group large yellow croaker skin of abdomen brightness value and control group there was no significant difference (P>0.05), but be significantly higher than wild fish (P<0.05).Skin of back brightness value and wild fish there was no significant difference (P>0.05), but be significantly higher than control group (P<0.05) (Fig. 2).Experimental group large yellow croaker belly red value and control group there was no significant difference (P>0.05), and significantly lower than wild fish (P<0.05) (Fig. 3).Experimental group skin of back red value and control group and wild fish there are no significant difference (P>0.05) (Fig. 4).The yellow value of experimental group large yellow croaker skin of abdomen and skin of back is lower than wild fish, but there was no significant difference (P>0.05), and be significantly higher than control group (P<0.05) (Fig. 5 and Fig. 6).Similar with yellow value, experimental group large yellow croaker skin of back and skin of abdomen pigment content are significantly higher than control group (P<0.05), lower than wild group but there was no significant difference (P>0.05) (Fig. 7 and Fig. 8).
Experimental result shows, large yellow croaker body colour reinforcing agent of the present invention can significantly improve the body colour of propagating large yellow croaker artificially, makes it close to wild large yellow croaker.And, by the proportioning between three kinds of compositions, can effectively reduce costs, there is good market popularization value.
In addition, in order better to embody the effect of body colour reinforcing agent of the present invention, the present invention has also prepared the feed of large yellow croaker specially, include the component of following mass percent: white fish meal 43%, dregs of beans 21%, wheat flour 20.75-20.9%, brewer's yeast 2%, fish oil 7.1%, soybean lecithin 1.5%, multidimensional 2% for large yellow croaker, large yellow croaker is with many ore deposits 2%, phagostimulant 0.3%, antioxidant 0.1%, mould inhibitor 0.1%.
The vitamin that wherein large yellow croaker can select arbitrary money market sale to use by multidimensional, its a kind of component following (kg): Cobastab
1, 25mg; Riboflavin, 45mg; Cobastab
6(puridoxine hydrochloride), 20mg; Cobastab
12, 0.1mg; Vitamin K
3, 10mg; Inositol, 800mg; Cobastab
3(pantothenic acid), 60mg; Nicotinic acid, 200mg; Folic acid, 20mg; Biotin, 1.20mg; Vitamin A, 32mg; Vitamin D
3, 5mg; Vitamin E, 120mg; Vitamin C, 2000mg; Ethoxyquinoline, 150mg; Inferior powder, 16.51g
Large yellow croaker can select arbitrary money market sale to use with many ore deposits, its one (kg) composed as follows: sodium fluoride, 2mg; KI, 18mg; Cobalt chloride (1 %), 50mg; Copper sulphate, 10mg; Ferric sulfate, 80mg; Zinc sulfate, 50mg; Manganese sulfate, 60mg; Magnesium sulfate, 1200mg; Calcium dihydrogen phosphate, 10g; Sodium chloride, 100mg; Zeolite powder, 8.45g.
Phagostimulant is selected the mixture of glycine and betaine, and its mass ratio is 1:1.
Mould inhibitor is selected the mixture of calcium propionate and fumaric acid, and its mass ratio is 1:1.
Large yellow croaker body colour reinforcing agent formula of the present invention is joined in this feed by the addition of 0.01-0.15%, use the content of carotenoid in the body colour value that just can significantly improve for about 8 weeks in large yellow croaker skin and skin, obviously improve the exterior quality of large yellow croaker.Compared with other feed, body colour and the wild large yellow croaker of the large yellow croaker of the body colour reinforcing agent of the present invention of simultaneously feeding are more approaching, make the large yellow croaker of raising have better economic worth.
Claims (4)
1. an application process for large yellow croaker body colour reinforcing agent, is that large yellow croaker body colour reinforcing agent is added in large yellow croaker feed, and the mass percent of interpolation is 0.01-0.15%; Described large yellow croaker feed, its mass percent is composed as follows: white fish meal 43%, dregs of beans 21%, wheat flour 20.9%, brewer's yeast 2%, fish oil 7.1%, soybean lecithin 1.5%, multidimensional 2%, many ore deposits 2%, phagostimulant 0.3%, ethoxyquinoline 0.1%, mould inhibitor 0.1%, the mass percent sum of said components is 100%;
Described body colour reinforcing agent is comprised of astaxanthin, lutein and canthaxanthin, and the mass ratio of astaxanthin, lutein and canthaxanthin is 20-45%, 20-45% and 20-45%.
2. application process as claimed in claim 1, is characterized in that the mass ratio of astaxanthin, lutein and canthaxanthin in described body colour reinforcing agent is 1:1:1.
3. application process as claimed in claim 1, is characterized in that described phagostimulant is the mixture of glycine and betaine, and wherein the mass ratio of glycine and betaine is 1:1.
4. application process as claimed in claim 1, is characterized in that described mould inhibitor is the mixture of calcium propionate and fumaric acid, and wherein the mass ratio of calcium propionate and fumaric acid is 1:1.
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| CN102657298B true CN102657298B (en) | 2014-04-23 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103141673B (en) * | 2013-02-04 | 2014-04-02 | 上海农好饲料有限公司 | Large yellow croaker buoyant puffing feed without fish meal and preparation method thereof |
| CN104082214B (en) * | 2014-07-16 | 2016-05-11 | 宁德市富发水产有限公司 | A kind of method that can promote large yellow croaker body surface its colour changed into yellow |
| CN104757285B (en) * | 2015-03-16 | 2018-01-30 | 中国水产科学研究院南海水产研究所 | A kind of mixed feed for improving egg-shaped pompano body colour |
| CN108208472B (en) * | 2018-03-21 | 2021-07-23 | 集美大学 | Feed additive, large yellow croaker feed and preparation method and application of feed additive and large yellow croaker feed |
| CN108813146A (en) * | 2018-05-31 | 2018-11-16 | 中国科学院海洋研究所 | A kind of Pseudosciaena crocea body colour modifier and preparation method thereof |
| CN109090376A (en) * | 2018-08-24 | 2018-12-28 | 天邦食品股份有限公司 | A kind of functional feed for imitating wild cultivation suitable for Larimichthys crocea |
| CN108902566A (en) * | 2018-09-18 | 2018-11-30 | 中国海洋大学 | A kind of rainbow trout culturing feed and its application |
| CN113678961A (en) * | 2021-08-13 | 2021-11-23 | 中国海洋大学 | Compound feed for improving body color of large yellow croaker and preparation method thereof |
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| CN101028042B (en) * | 2007-02-06 | 2010-07-14 | 福建天马饲料有限公司 | Slow-settling swelling granular complex feed for large-yellow croaker |
| CN101411395A (en) * | 2008-11-05 | 2009-04-22 | 广东恒兴集团有限公司 | Feedstuff additive capable of improving body color of aquatic animal |
| CN101897384A (en) * | 2009-05-31 | 2010-12-01 | 中国热带农业科学院热带作物品种资源研究所 | Method for processing natural plant feed coloring agent |
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