CN102656448A - 用于凝胶电泳、成像和分析的方法、装置、系统和材料 - Google Patents
用于凝胶电泳、成像和分析的方法、装置、系统和材料 Download PDFInfo
- Publication number
- CN102656448A CN102656448A CN2010800463501A CN201080046350A CN102656448A CN 102656448 A CN102656448 A CN 102656448A CN 2010800463501 A CN2010800463501 A CN 2010800463501A CN 201080046350 A CN201080046350 A CN 201080046350A CN 102656448 A CN102656448 A CN 102656448A
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- China
- Prior art keywords
- gel
- electrophoresis
- certain embodiments
- image
- light source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
- G01N27/44778—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis on a common gel carrier, i.e. 2D gel electrophoresis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23629309P | 2009-08-24 | 2009-08-24 | |
| US61/236,293 | 2009-08-24 | ||
| US23679509P | 2009-08-25 | 2009-08-25 | |
| US61/236,795 | 2009-08-25 | ||
| US23728709P | 2009-08-26 | 2009-08-26 | |
| US23719509P | 2009-08-26 | 2009-08-26 | |
| US61/237,195 | 2009-08-26 | ||
| US61/237,287 | 2009-08-26 | ||
| PCT/US2010/046491 WO2011025781A1 (en) | 2009-08-24 | 2010-08-24 | Gel electrophoresis, imaging, and analysis methods, devices, systems, and materials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102656448A true CN102656448A (zh) | 2012-09-05 |
Family
ID=43604426
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201080046343.1A Active CN102648309B (zh) | 2009-08-24 | 2010-08-24 | 电泳凝胶盒以及电泳梳 |
| CN2010800463501A Pending CN102656448A (zh) | 2009-08-24 | 2010-08-24 | 用于凝胶电泳、成像和分析的方法、装置、系统和材料 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201080046343.1A Active CN102648309B (zh) | 2009-08-24 | 2010-08-24 | 电泳凝胶盒以及电泳梳 |
Country Status (6)
| Country | Link |
|---|---|
| US (8) | US20110042217A1 (enExample) |
| EP (5) | EP2470891B1 (enExample) |
| JP (4) | JP2013502597A (enExample) |
| CN (2) | CN102648309B (enExample) |
| IN (2) | IN2012DN01825A (enExample) |
| WO (2) | WO2011025781A1 (enExample) |
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| WO2016019482A1 (en) * | 2014-08-06 | 2016-02-11 | Coyote Bioscience Co., Ltd. | Electrophoresis apparatus |
| CN105734604A (zh) * | 2016-03-03 | 2016-07-06 | 武汉大学 | 一种立体复合多糖凝胶及其电化学3d打印制备方法和应用 |
| CN105955408A (zh) * | 2016-05-18 | 2016-09-21 | 张家港润盛科技材料有限公司 | 一种笔记本铝制键盘基板 |
| CN106290153A (zh) * | 2015-06-25 | 2017-01-04 | 株式会社日立高新技术 | 自动分析装置及摄像方法 |
| CN110214268A (zh) * | 2017-01-26 | 2019-09-06 | 蔚蓝生物系统有限公司 | 用于成像生物分子的装置和方法 |
| US10465239B2 (en) | 2013-12-25 | 2019-11-05 | Coyote Bioscience Co., Ltd. | Methods and systems for nucleic acid amplification |
| CN110988094A (zh) * | 2019-12-24 | 2020-04-10 | 武汉启程嘉辉科技有限公司 | 一种迷你双垂直电泳仪 |
| CN111465858A (zh) * | 2017-12-15 | 2020-07-28 | 生物辐射实验室股份有限公司 | 测定的组合成像 |
| CN115131784A (zh) * | 2022-04-26 | 2022-09-30 | 东莞博奥木华基因科技有限公司 | 一种图像处理方法、装置、电子设备及存储介质 |
| CN115440314A (zh) * | 2022-09-06 | 2022-12-06 | 湖南艾科瑞生物工程有限公司 | 琼脂糖凝胶的电泳性能检测方法及相关设备 |
| CN115937052A (zh) * | 2023-03-14 | 2023-04-07 | 四川福莱宝生物科技有限公司 | 一种凝胶电泳图像的处理方法、装置、设备及介质 |
| CN116482064A (zh) * | 2022-10-31 | 2023-07-25 | 上海交通大学 | 用于蛋白质凝胶电泳的内源性荧光成像检测系统及方法 |
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| CN102648309B (zh) | 2009-08-24 | 2015-08-12 | 生命技术公司 | 电泳凝胶盒以及电泳梳 |
| US8715476B2 (en) * | 2009-12-11 | 2014-05-06 | Bio-Rad Laboratories, Inc. | Instrument for independent electrotransfer in multiple cassettes |
| WO2012012382A1 (en) * | 2010-07-20 | 2012-01-26 | Dow Agrosciences Llc | Systems and methods to analyze an immunoassay test strip comb member |
| USD698037S1 (en) * | 2012-06-01 | 2014-01-21 | Life Technologies Corporation | Electrophoresis tank |
| USD667134S1 (en) * | 2011-04-01 | 2012-09-11 | Life Technologies Corporation | Electrophoresis apparatus |
| USD794823S1 (en) | 2010-08-24 | 2017-08-15 | Life Technologies Corporation | Electrophoresis tank with a base and cassette inserted in |
| USD719277S1 (en) | 2010-08-24 | 2014-12-09 | Life Technologies Corporation | Electrophoresis wedge-well comb |
| US9234874B2 (en) * | 2011-02-24 | 2016-01-12 | Bio-Rad Laboratories, Inc. | Dimensional stabilization of slab gel cassettes to prevent distortion caused by swelling gels |
| US20120247958A1 (en) * | 2011-04-01 | 2012-10-04 | Life Technologies Corporation | Gel processing systems for electrophoresis, imaging, and analysis, software and methods |
| WO2012149180A2 (en) * | 2011-04-26 | 2012-11-01 | Life Technologies Corporation | Fluorescent tracking dye |
| US20130111404A1 (en) * | 2011-11-02 | 2013-05-02 | Microsoft Corporation | User interface for saving documents using external storage services |
| WO2014007720A1 (en) * | 2012-05-31 | 2014-01-09 | Ge Healthcare Bio-Sciences Ab | An electrophoresis gel unit comprising a flat gel member attached to a support |
| CN103512871B (zh) * | 2012-06-28 | 2017-04-12 | 上海百可生物科技股份有限公司 | 一种细菌耐药性荧光检测方法及其诊断试剂盒 |
| GB2506425A (en) * | 2012-09-28 | 2014-04-02 | Major Science Co Ltd | Electrophoresis imaging system |
| US9061236B2 (en) * | 2012-11-20 | 2015-06-23 | Nanjingjinsirui Science & Technology Biology Corporation | Gel electrophoresis device for loading large sample volumes |
| CN103234907B (zh) * | 2013-04-16 | 2016-01-13 | 无锡优创生物科技有限公司 | 基于压力感应的凝胶跟踪装置及凝胶成像分析系统 |
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| CN103698381B (zh) * | 2013-11-27 | 2016-04-06 | 杰弗瑞·陈 | 电泳用预制胶基片、基于基片的聚丙烯酰胺凝胶预制胶及其使用方法 |
| CN104764789B (zh) * | 2013-12-17 | 2018-02-23 | 百奇生物科技(苏州)有限公司 | 凝胶容器的结构及其制备方法 |
| JP6771801B2 (ja) | 2014-03-28 | 2020-10-21 | サイティバ・スウェーデン・アクチボラグ | 電気泳動分離の方法 |
| WO2015151768A1 (ja) * | 2014-03-31 | 2015-10-08 | シャープ株式会社 | サンプル分離吸着用分離媒体カセットおよびサンプル分離吸着用分析装置 |
| US9835587B2 (en) * | 2014-04-01 | 2017-12-05 | C.C. Imex | Electrophoresis running tank assembly |
| JP2016102715A (ja) * | 2014-11-28 | 2016-06-02 | 富士フイルム株式会社 | 撮影装置およびその制御方法並びに撮影システム |
| EP3234578A4 (en) * | 2014-12-16 | 2020-11-25 | Bio-Rad Laboratories, Inc. | HORIZONTAL ELECTROPHORESIS SEPARATION DEVICE WITHOUT SEALING AND GEL EXTRACTION PROCESS WITHOUT OPENING A CASSETTE |
| USD806894S1 (en) | 2015-03-19 | 2018-01-02 | Life Technologies Corporation | Electrophoresis comb with wedge-shaped teeth |
| CN105466993A (zh) * | 2015-09-14 | 2016-04-06 | 青岛爱迪生物科技有限公司 | 一种快速蛋白电泳缓冲液 |
| USD849963S1 (en) | 2015-11-13 | 2019-05-28 | Life Technologies Corporation | Electrophoresis gel cassette |
| US10359396B2 (en) | 2015-11-13 | 2019-07-23 | Life Technologies Corporation | Preparation of electrophoresis gels, and related devices, systems, and methods |
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| US20180372680A1 (en) * | 2017-06-26 | 2018-12-27 | Bio-Rad Laboratories, Inc. | Gel cassette opener |
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| JP7167531B2 (ja) * | 2018-08-03 | 2022-11-09 | 株式会社島津製作所 | 電気泳動分離データ解析装置、電気泳動分離データ解析方法及びその解析方法をコンピュータに実施させるためのコンピュータプログラム |
| WO2021030427A2 (en) * | 2019-08-12 | 2021-02-18 | Yale University | Intragel well sample loading system |
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