CN102631021B - Two-stage enzyme treatment method for improving stalk shred smoking quality - Google Patents
Two-stage enzyme treatment method for improving stalk shred smoking quality Download PDFInfo
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- CN102631021B CN102631021B CN2012100994959A CN201210099495A CN102631021B CN 102631021 B CN102631021 B CN 102631021B CN 2012100994959 A CN2012100994959 A CN 2012100994959A CN 201210099495 A CN201210099495 A CN 201210099495A CN 102631021 B CN102631021 B CN 102631021B
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- hemicellulase
- laccase
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Abstract
The invention discloses a two-stage enzyme treatment method for improving stalk shred smoking quality. First-stage enzyme treatment aims at the tobacco stalk, and second-stage enzyme treatment aims at the stalk shred. The method comprises the following steps of: soaking the washed tobacco stalk in a first-stage enzyme preparation for 10-20 seconds, taking out and storing at the temperature of 20-50 DEG C for 2 hours, and shredding after the stalk is evaporated; and according to the dosage that 50ml of enzyme liquid is sprayed onto 1kg of the stalk shred, evenly spraying the second-stage enzyme treatment preparation onto the stalk shred, and carrying out pneumatic drying on the stalk shred at the temperature of 180 DEG C after storing for 2 hours. The treated stalk shred has the advantages that the oral comfort can be increased, and the impure gas and the stimulus can be reduced, so that the smoke is softer. The method is high in practical applicability, the use ratio of the stalk shred can be improved, and the tobacco leaf resource can be saved.
Description
Technical field
The invention belongs to technical field of tobacco processing, be specifically related to a kind of method of processing to improve the stem sucking quality by the two-stage enzyme.
Background technology
Offal is the important component part of tobacco leaf.Yet, because the cell wall substance contents such as cellulose, hemicellulose, pectin and lignin in offal are higher, after causing the offal burning, produce strong impulse, assorted gas is heavy, few, the puckery mouth of perfume quantity, restricted the usage ratio of stem in cigarette composition, cause at present domestic lowly to offal resource comprehensive interest rate, idle offal waste is larger.Therefore, in the Cigarette processing process, taking adequate measures to promote cell wall substance degraded in offal, is necessary to improving stem suction flavor.
In recent years, China is utilizing enzyme preparation to improve aspect cabo quality the research of having carried out some explorations.If in patent 200510104443.6, offal immersed in complex enzyme liquid, then store, and offal is steamed and obstructs the enzyme that goes out, promote colour generation, be fragrant, be the flavor reaction, smoking result shows that the fragrance of stem is promoted, and excitant also makes moderate progress.After Zhou Yuanqing etc. (2006) utilize lignin degradation microorganism and lignin-degrading enzymes to process offal, reduced the excitant of stem, the filling value also is improved.
Offal is carried out to the enzyme processing, although can destroy to a certain extent the cell wall structure of offal, make offal more soft, be easy to later stage pressure stalk, chopping.Yet, the plant cell wall complex structure, lignin is combined with the covalent bond form with hemicellulose, by the cellulosic molecule embedding wherein.Therefore, only for offal, carry out an enzyme and process, very limited to the degradation effect of cell wall substance.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of method of improving the stem smoking quality is provided, in prior art, utilize complex enzyme offal to be carried out on the basis of single treatment, for cutting stem after stalk, carry out enzyme for the second time and process again, further reduce cell wall substance content, improve the suction quality of stem.
Purpose of the present invention is achieved by the following technical programs.
Except as otherwise noted, percentage of the present invention is percetage by weight.
A kind of two-stage method of enzymatically treating that improves the stem smoking quality comprises the following steps:
(1) take pectase, cellulase, hemicellulase, laccase and water, after mixing, make first order enzyme preparation, wherein, the weight percent content of each component in enzyme preparation is: pectase 0.01%~1.0%, cellulase 0.01%~1.0%, hemicellulase 0.01%~1.0% and laccase 0.01%~1.0%, surplus is water, and the complex enzyme liquid temp is controlled at 45 ℃ ± 5 ℃;
The preferred component content of above-mentioned first order enzyme preparation is: pectase 0.5%, cellulase 0.5%, hemicellulase 0.5% and laccase 0.5%, surplus are water.
The preferred component content of above-mentioned first order enzyme preparation is: pectase 1.0%, cellulase 0.3%, hemicellulase 0.3% and laccase 0.5%, surplus are water.
(2) offal after washing immersed in the first order enzyme preparation of step (1) preparation 10~20 seconds, took out, and stored 2 hours at 20~50 ℃ of temperature;
(3) under the condition of 80 ℃~100 ℃, steamed stalk 3~5 seconds;
(4) steam the offal chopping after pressing stalk after obstructing;
(5) take pectase, cellulase, hemicellulase, laccase, protease, amylase and water, after mixing, make second level enzyme preparation, wherein, the weight percent content of each component in enzyme preparation is: pectase 0.02%~0.2%, cellulase 0.02%~0.2%, hemicellulase 0.02%~0.2%, laccase 0.02%~0.2%, proteinase-10 .02%~0.2%, amylase 0.02%~0.2%, surplus is water, and the complex enzyme liquid temp is controlled at 45 ℃ ± 5 ℃;
The preferred component content of above-mentioned second level enzyme preparation is: pectase 0.2%, cellulase 0.2%, hemicellulase 0.2%, laccase 0.2%, proteinase-10 .2%, amylase 0.2%.
The preferred component content of above-mentioned second level enzyme preparation is: pectase 0.02%, cellulase 0.02%, hemicellulase 0.02%, laccase 0.02%, proteinase-10 .02%, amylase 0.02%.
(6) second level enzyme preparation step (5) obtained evenly is sprayed on stem, and every 1kg stem sprays the 50ml enzyme preparation, stores 2 hours at 20~50 ℃ of temperature;
(7) under 180 ℃ of conditions, stem is carried out to pneumatic conveying drying.
The present invention can make the content of the cell wall substance in offal obviously reduce, and the content of total reducing sugar and reduced sugar raises, and the fragrance of stem, assorted gas and harmony have obtained larger improvement, and excitant reduces, and has reached the purpose that promotes the stem suction quality.
The accompanying drawing explanation
Fig. 1 is process chart of the present invention.
The specific embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail, but drawings and Examples are not limited to the technical solution.All conversion of making based on training centre of the present invention, all should belong to protection scope of the present invention.
Embodiment 1
By following percentage by weight, each component is dissolved in suitable quantity of water: pectase 0.5%, hemicellulase 0.5%, cellulase 0.5% and laccase 0.5%.Filter, filtrate is settled to volume required.The complex enzyme liquid temp is controlled at 45 ℃ ± 5 ℃.Offal after washing was immersed in the complex enzyme liquid prepare 10~20 seconds, and took out, stored 2 hours for 20~50 ℃.Then 80 ℃~100 ℃ were steamed stalk 3~5 seconds.
Offal cell wall substance after complex enzyme is processed, comprise that pectin, cellulose, hemicellulose and content of lignin all decrease; The chemical composition testing result shows, complex enzyme process after reduced sugar and the total sugar content of offal raise, be conducive to the lifting of stem suction quality.
Table 1 one-level enzyme is processed the cell wall substance content of rear offal
Embodiment 2
From the difference of embodiment 1, be that the percentage by weight of each component in complex enzyme liquid is different, its each component is respectively pectase 1.0%, hemicellulase 0.3%, cellulase 0.3% and laccase 0.5%.Its technical process is identical with embodiment 1.
Embodiment 3
By offal chopping after pressing stalk of steaming in embodiment 1 after stalk is processed.Percentage by weight by following enzyme and stem is dissolved in each component in suitable quantity of water: pectase 0.2%, hemicellulase 0.2%, cellulase 0.2%, laccase 0.2%, proteinase-10 .2%, amylase 0.2%, filter, and filtrate is settled to volume required.Press the 1kg stem and spray 50ml enzyme liquid consumption, enzyme liquid evenly is sprayed on stem, preserved 2 hours.180 ℃ of temperature are carried out pneumatic conveying drying to stem.
Stem cell wall substance after the secondary complex enzyme is processed, comprise that pectin, cellulose, hemicellulose and content of lignin all decrease, can reduce when insufficient by the burning of cellulose, lignin produce penetrating odor, simultaneously, the bad breaths such as xylon gas that produced by lignin and pectin burning also can be minimized.
The chemical composition testing result shows, complex enzyme process after reduced sugar and the total sugar content of stem raise, be conducive to the lifting of stem suction quality.
Smoking result shows, the stem fragrance after complex enzyme is processed, assorted gas and harmony have obtained larger improvement; Excitant and xylon gas reduce, and suction quality is promoted.
Table 2 secondary enzyme is processed the cell wall substance content of rear stem
Embodiment 4
From the difference of embodiment 3, be that the percentage by weight of each component in complex enzyme liquid is different, its each component is respectively pectase 0.02%, cellulase 0.02%, hemicellulase 0.02%, laccase 0.02%, proteinase-10 .02%, amylase 0.02%.Its technical process is identical with embodiment 3.
Stem after the secondary enzyme is processed has relaxed the excitant of flue gas, and the oral cavity comfortableness strengthens, and assorted gas reduces, and the fragrance of stem, assorted gas and harmony have obtained larger improvement.
Claims (5)
1. two-stage method of enzymatically treating that improves the stem smoking quality comprises the following steps:
(1) take pectase, cellulase, hemicellulase, laccase and water, after mixing, make first order enzyme preparation, wherein, the weight percent content of each component in enzyme preparation is: pectase 0.01%~1.0%, cellulase 0.01%~1.0%, hemicellulase 0.01%~1.0% and laccase 0.01%~1.0%, surplus is water, and the complex enzyme liquid temp is controlled at 45 ℃ ± 5 ℃;
(2) offal after washing immersed in the first order enzyme preparation of step (1) preparation 10~20 seconds, took out, and stored 2 hours at 20~50 ℃ of temperature;
(3) under 80 ℃~100 ℃ conditions, steamed stalk 3~5 seconds;
(4) steam the offal chopping after pressing stalk after obstructing;
(5) take pectase, cellulase, hemicellulase, laccase, protease, amylase and water, after mixing, make second level enzyme preparation, wherein, each weight percent content that forms in enzyme preparation is: pectase 0.02%~0.2%, cellulase 0.02%~0.2%, hemicellulase 0.02%~0.2%, laccase 0.02%~0.2%, proteinase-10 .02%~0.2%, amylase 0.02%~0.2%, surplus is water, and the complex enzyme liquid temp is controlled at 45 ℃ ± 5 ℃;
(6) second level enzyme preparation step (5) obtained evenly is sprayed on stem, and every 1kg stem sprays the 50ml enzyme preparation, stores 2 hours at 20~50 ℃ of temperature;
(7) under 180 ℃ of conditions, stem is carried out to pneumatic conveying drying.
2. the two-stage method of enzymatically treating that improves the stem smoking quality according to claim 1, it is characterized in that: the preferred component content of the first order enzyme preparation described in step (1) is: pectase 0.5%, cellulase 0.5%, hemicellulase 0.5% and laccase 0.5%, surplus are water.
3. the two-stage method of enzymatically treating that improves the stem smoking quality according to claim 1, it is characterized in that: the preferred component content of the first order enzyme preparation described in step (1) is: pectase 1.0%, cellulase 0.3%, hemicellulase 0.3% and laccase 0.5%, surplus are water.
4. the two-stage method of enzymatically treating that improves the stem smoking quality according to claim 1, it is characterized in that: the preferred component content of the second level enzyme preparation described in step (5) is: pectase 0.2%, cellulase 0.2%, hemicellulase 0.2%, laccase 0.2%, proteinase-10 .2%, amylase 0.2%, surplus are water.
5. the two-stage method of enzymatically treating that improves the stem smoking quality according to claim 1, it is characterized in that: the preferred component content of the second level enzyme preparation described in step (5) is: pectase 0.02%, cellulase 0.02%, hemicellulase 0.02%, laccase 0.02%, proteinase-10 .02%, amylase 0.02%, surplus are water.
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CN102894472B (en) * | 2012-09-29 | 2014-12-24 | 福建中烟工业有限责任公司 | Method for improving sensory quality of tobacco leaves by using compound enzymic preparation |
CN102894468A (en) * | 2012-10-19 | 2013-01-30 | 云南瑞升烟草技术(集团)有限公司 | Preparation method of functional additive capable of improving cut stem quality |
CN103070469B (en) * | 2013-01-17 | 2016-01-20 | 广东中烟工业有限责任公司 | A kind of enzyme solution of offal processing |
CN103070474B (en) * | 2013-01-17 | 2015-03-18 | 广东中烟工业有限责任公司 | Tobacco stalk processing method for enhancing fragrance by tobacco stalks |
CN103462212B (en) * | 2013-09-05 | 2015-04-08 | 广西中烟工业有限责任公司 | Method for improving quality of tobacco leaves by adding enzyme preparation, glucose and citric acid |
CN106723297B (en) * | 2017-02-24 | 2018-03-09 | 云南中烟工业有限责任公司 | A kind of preparation method of ramuscule cigarette cut stem |
CN110742303A (en) * | 2019-10-22 | 2020-02-04 | 红塔辽宁烟草有限责任公司 | Complex enzyme preparation for improving smoking quality of tobacco flakes and application thereof |
CN112852792B (en) * | 2019-11-28 | 2022-07-19 | 中国科学院微生物研究所 | Complex enzyme for degrading tobacco stems and application of complex enzyme in degrading tobacco stems |
CN114766713A (en) * | 2022-04-30 | 2022-07-22 | 中国烟草总公司郑州烟草研究院 | Cigarette cut tobacco making process based on improvement of permeability of surface layer of tobacco lamina |
CN115161123A (en) * | 2022-07-21 | 2022-10-11 | 陕西中烟工业有限责任公司 | Cleaning agent for cleaning tobacco stems and preparation method and application thereof |
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CN101711600B (en) * | 2008-10-08 | 2012-04-25 | 武汉达汇创新科技有限公司 | Process for biochemically treating cut stems |
CN101912152B (en) * | 2010-08-13 | 2012-06-27 | 川渝中烟工业有限责任公司 | Method for improving smoking quality by treating steam-exploded tobacco stalks with complex enzyme |
CN102247009B (en) * | 2011-07-12 | 2013-04-10 | 广东中烟工业有限责任公司 | Maillard reaction process for tobacco stalk extract |
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