CN101912152B - Method for improving smoking quality by treating steam-exploded tobacco stalks with complex enzyme - Google Patents
Method for improving smoking quality by treating steam-exploded tobacco stalks with complex enzyme Download PDFInfo
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- CN101912152B CN101912152B CN2010102524130A CN201010252413A CN101912152B CN 101912152 B CN101912152 B CN 101912152B CN 2010102524130 A CN2010102524130 A CN 2010102524130A CN 201010252413 A CN201010252413 A CN 201010252413A CN 101912152 B CN101912152 B CN 101912152B
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Abstract
The invention relates to a method for improving smoking quality by treating steam-exploded tobacco stalks with a complex enzyme. The method comprises the following steps of: compounding single enzymes such as pectinase, cellulase, hemicellulase, xylanase, laccase, amylase and saccharifying enzyme, adding an appropriate amount of water into the mixed enzyme and spraying the mixed enzyme on exploded tobacco stalks, wherein in this process, the water content of the tobacco stalks is controlled to be between 40 and 50 percent, treatment temperature is between 40 and 50 DEG C and stalk storage time is between 4 and 10 hours; and performing enzyme inactivation on the treated tobacco stalks at the temperature of between 70 and 90 DEG C for 20 to 40 minutes. The method has the advantages of improving the smoking quality of the tobacco stalks, increasing the utilization ratio of the tobacco stalks in cigarette production and effectively lowering cigarette cost.
Description
Technical field
The present invention relates to a kind of applying composite enzyme and handle the method that the steam blasting offal improves sucking quality, belong to the tobacco manufacture field.
Background technology
Offal is the important component part of tobacco leaf, generally accounts for about 1/4 of full wafer quality of tobacco.Cell wall substance contents such as cellulose, hemicellulose, lignin and pectin account for 43.8% of dry biomass in the offal.Cell wall substance be in the carbohydrate to the unfavorable factor of tobacco interior quality and local flavor influence, this material pyrolysis meeting produces more low-grade aldehydes, when burning and sucking, can produce irritating choking and cough.Cellulose is combustion-supporting, but excitant of giving the flue gas spine and " burning paper as sacrificial offerings " flavor, and the degree of polymerization is high more, and negatively influencing is big more, and lignin produces the main cause of the larynx that burns when being considered to cause the heavy and burning of stem xylon gas.In addition, offal pyrolysis meeting produces catechol and alkyl catechol, causes puckery mouthful and short cancer activity.Pectic substance is a hydrophilic colloid, and the hygroscopicity and the elasticity of tobacco leaf are played a role, and can produce methyl alcohol but decompose.The cell wall substance content height, reduced sugar and water-soluble total sugar content are lower, cause tobacco leaf that strong impulse property is arranged, and assorted gas is heavy, the suction flavor is choked coughs, puckery mouth, and perfume quantity is few.Cellulose, hemicellulose, lignin and pectin content are higher, cause offal to have strong impulse property, and assorted gas is heavy, the suction flavor is choked coughs, puckery mouth, and perfume quantity is few, has restricted the usage ratio of stem in cigarette composition.Simultaneously, the content of endogenous enzymes is less in the offal, is not enough to transform the lignin in the offal, cellulose, pectin etc. fully at short notice.Therefore, in the cigarette process, utilize suitable exogenous enzymes regulation and control, promote the enzymolysis of offal main chemical compositions, the sucking quality that improves offal is necessary.
Summary of the invention
The purpose of this invention is to provide a kind of applying composite enzyme and handle the method that the steam blasting offal improves sucking quality,, improve the offal sucking quality to promote the conversion of cellulose, hemicellulose and lignin etc. in the offal.
The objective of the invention is to realize like this: a kind of applying composite enzyme is handled the method that the steam blasting offal improves quality, and the technological parameter and the complex enzyme processing composite, the enzyme effect of single enzyme combine with the steam blasting reasonable in technology, and this method may further comprise the steps:
A, at first single enzyme is carried out by a certain percentage composite, above-mentioned single enzyme is: pectase, cellulase, hemicellulase, zytase, laccase, amylase and carbohydrase;
The compound proportion of b, enzyme: pectase: cellulase: hemicellulase: zytase: laccase: amylase: carbohydrase=(5～100U): (5～100U): (5～100U): (5～100U): (5～100U): (1000～1000000U): (1000～1000000U);
C, then offal behind the steam blasting is carried out complex enzyme and handle; Technological parameter: offal moisture is 40～50%, and processing environment humidity is 80～90%, 40～50 ℃ of treatment temperatures, time 4～10h;
Offal after d, above-mentioned enzyme handle carries out 70～90 ℃, the high temperature of the 20～40min enzyme that goes out; The enzyme activity determination of above-mentioned single enzyme adopts following method: cellulase adopts the filter paper enzyme process; Pectase is measured the hypoiodous acid sodium method that adopts; Hemicellulase and zytase adopt the DNS method; The laccase vitality test adopts guaiacol method, and it is the DNS method that amylase and carbohydrase adopt.
The compound proportion of above-mentioned enzyme is: cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U; Steam blasting is that the employing saturated steam is a working media, and explosion treatment pressure is 0.8MPa, and the pressure retention time is 20 seconds; The technological parameter that complex enzyme is handled: offal moisture 50%, 50 ℃ of temperature, time 6h; Carry out 80 ℃, the 20min enzyme that goes out at last.
The compound proportion of above-mentioned enzyme is: cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U; Steam blasting is that the employing saturated steam is a working media, and explosion treatment pressure is 0.8MPa, and the pressure retention time is 20 seconds; The technological parameter that complex enzyme is handled: offal moisture 40%, 40 ℃ of temperature, action time 6h; Carry out 80 ℃, the 20min enzyme that goes out at last.
The compound proportion of above-mentioned enzyme is: cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U; Steam blasting is that the employing saturated steam is a working media, and explosion treatment pressure is 0.8MPa, and the pressure retention time is 20 seconds; The technological parameter that complex enzyme is handled: offal moisture 45%, 45 ℃ of temperature, action time 10h; Carry out 80 ℃, the 20min enzyme that goes out at last.
The invention has the beneficial effects as follows:
Because the composition of offal is complicated, wherein cellulose, hemicellulose, pectin, lignin occupy larger specific gravity again, and it is not obvious to utilize single enzyme that it is carried out treatment effect, and consuming time.Single a kind of enzyme preparation of use in the existing process conditions; Like pectase or cellulase, have certain effect for the degraded of cell wall substance, but because cell wall substance is the composition of a complicacy; Lignin works the effect of connection with hemicellulose, and these become branch to hinder the action effect of enzyme.
The present invention carries out steam blasting to offal earlier, and the lignocellulosic structure is dissociated, and institutional framework is loose, is easy to complex enzyme formulation infiltration and opposition effect targetedly, improves hydrolysis result, reduces lignin, fiber and pectic substance content.
The present invention utilizes the cooperative effect between the complex enzyme system to handle the explosion offal, improves the efficient of enzymolysis, makes effect more remarkable.The amount of its reduced sugar and total reducing sugar increases significantly, and cell wall substance obviously descends, and sucking quality has clear improvement.Applying composite enzyme is handled the steam blasting offal, between them cooperative effect can take place, and can fully destroy structures such as lignin, cellulose; Promote conversions such as cellulose, hemicellulose and lignin; Improve content of reducing sugar, promote the release of flavor components, improve the sucking quality of its cigarette.
The specific embodiment
Applying composite enzyme of the present invention is handled the method that the steam blasting offal improves quality, and the technological parameter and the complex enzyme processing composite, the enzyme effect of single enzyme combine with the steam blasting reasonable in technology, and this method may further comprise the steps:
A, at first single enzyme is carried out by a certain percentage composite, above-mentioned single enzyme is: pectase, cellulase, hemicellulase, zytase, laccase, amylase and carbohydrase;
The compound proportion of b, enzyme: pectase: cellulase: hemicellulase: zytase: laccase: amylase: carbohydrase=(5～100U): (5～100U): (5～100U): (5～100U): (5～100U): (1000～1000000U): (1000～1000000U);
C, then offal behind the steam blasting is carried out complex enzyme and handle; Technological parameter: offal moisture is 40～50%, and processing environment humidity is 80～90%, 40～50 ℃ of temperature, time 4～10h;
Offal after d, last enzyme are handled carries out 70～90 ℃, the high temperature of the 20～40min enzyme that goes out;
The basic assurance that realizes this method is an activity of measuring a various enzyme earlier, adds according to the ratio weighing alive of various enzymes then.More even for the interpolation that makes enzyme, need a kind of press atomization device; When Terrier Storage, avoid temperature and humidity that bigger fluctuation is arranged; The effect of laccase needs oxygen, so in processing procedure, the additional air that be interrupted.
Embodiment 1:
What adopt is the steam blasting offal of 0.8MPa, 20s; Add single enzyme (cellulase, pectase, zytase) and complex enzyme (cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U) make an experiment; Its experimental condition: 50 ℃ of moisture 50%, temperature, action time 6h.After enzyme action time finishes, carry out 80 ℃, the 20min enzyme that goes out.Dry by the fire suitable moisture then, utilize the flow analysis appearance to carry out the detection of reduced sugar, and beat cigarette and carry out the sensory evaluating smoking.
| Reduced sugar increment % | Excitant | Mouth burns | Pleasant impression | Assorted gas | Total points | |
| Blank | 5.0 | 5.0 | 5.0 | 5.0 | 20 | |
| Cellulase | 16.31 | 5.3 | 5.2 | 5.3 | 5.4 | 21.2 |
| Pectase | 17.29 | 5.3 | 5.5 | 5.5 | 5.5 | 21.8 |
| Zytase | 10.08 | 5.1 | 5.3 | 5.2 | 5.2 | 20.8 |
| Complex enzyme | 17.94 | 5.2 | 5.3 | 5.4 | 5.2 | 21.1 |
Embodiment 2:
What adopt is the steam blasting offal of 0.8MPa, 20s; Add single enzyme (cellulase, pectase, zytase) and complex enzyme (cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U) make an experiment; Its experimental condition: 40 ℃ of moisture 40%, temperature, action time 6h.After enzyme action time finishes, carry out 80 ℃, the 20min enzyme that goes out.Dry by the fire suitable moisture then, utilize the flow analysis appearance to carry out the detection of reduced sugar, and beat cigarette and carry out the sensory evaluating smoking.
| Reduced sugar increment % | Excitant | Mouth burns | Pleasant impression | Assorted gas | Total points | |
| Blank | 5.0 | 5.0 | 5.0 | 5.0 | 20 | |
| Cellulase | 3.96 | 5.1 | 5.1 | 5.0 | 5.1 | 20.3 |
| Pectase | 4.34 | 5.2 | 5.3 | 5.2 | 5.2 | 20.9 |
| Zytase | 2.51 | 5.1 | 5.1 | 5.0 | 5.2 | 20.2 |
| Complex enzyme | 8.18 | 5.3 | 5.4 | 5.0 | 5.3 | 21.0 |
Embodiment 3:
What adopt is the steam blasting offal of 0.8MPa, 20s; Add single enzyme (cellulase, pectase, zytase) and complex enzyme (cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U) make an experiment; Its experimental condition: 45 ℃ of moisture 45%, temperature, action time 10h.After enzyme action time finishes, carry out 80 ℃, the 20min enzyme that goes out.Dry by the fire suitable moisture then, utilize the flow analysis appearance to carry out the detection of reduced sugar, and beat cigarette and carry out the sensory evaluating smoking.
| Reduced sugar increment % | Excitant | Mouth burns | Pleasant impression | Assorted gas | Total points | |
| Blank | -- | 5.0 | 5.0 | 5.0 | 5.0 | 20 |
| Cellulase | 5.65 | 5.1 | 5.2 | 5 | 5.1 | 20.4 |
| Pectase | 9.33 | 5.1 | 5.1 | 5.2 | 5.2 | 20.6 |
| Zytase | 4.84 | 5.1 | 5.2 | 5.1 | 5 | 20.4 |
| Complex enzyme | 12.92 | 5.2 | 5.2 | 5.4 | 5.3 | 21.1 |
Steam pressure is gauge pressure among this paper.
Claims (5)
1. an applying composite enzyme is handled the method that the steam blasting offal improves quality, it is characterized in that: the technological parameter and the complex enzyme processing composite, the enzyme effect of single enzyme combine with the steam blasting reasonable in technology, and this method may further comprise the steps:
A, at first single enzyme is carried out by a certain percentage composite, above-mentioned single enzyme is: pectase, cellulase, hemicellulase, zytase, laccase, amylase and carbohydrase;
The compound proportion of b, enzyme: pectase: cellulase: hemicellulase: zytase: laccase: amylase: carbohydrase=(5～100U): (5～100U): (5～100U): (5～100U): (5～100U): (1000～1000000U): (1000～1000000U);
C, then offal behind the steam blasting is carried out complex enzyme and handle; Technological parameter: offal moisture is 40～50%, and processing environment humidity is 80～90%, and treatment temperature is 40～50 ℃, time 4～10h;
Offal after d, last enzyme are handled carries out 70～90 ℃, the high temperature of the 20～40min enzyme that goes out.
2. improve the method for quality according to the said offal of claim 1, it is characterized in that: the enzyme activity determination of said single enzyme adopts following method: cellulase adopts the filter paper enzyme process; Pectase is measured the hypoiodous acid sodium method that adopts; Hemicellulase and zytase adopt the DNS method; The laccase vitality test adopts guaiacol method, and it is the DNS method that amylase and carbohydrase adopt.
3. improve the method for quality according to the said offal of claim 1, it is characterized in that: the compound proportion of said enzyme is: cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U; Said steam blasting is that the employing saturated steam is a working media, and explosion treatment pressure is 0.8MPa, and the pressure retention time is 20 seconds; The technological parameter that complex enzyme is handled: offal moisture 50%, 50 ℃ of temperature, time 6h; Carry out 80 ℃, the 20min enzyme that goes out at last.
4. improve the method for quality according to the said offal of claim 1, it is characterized in that: the compound proportion of said enzyme is: cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U; Said steam blasting is that the employing saturated steam is a working media, and explosion treatment pressure is 0.8MPa, and the pressure retention time is 20 seconds; The technological parameter that complex enzyme is handled: offal moisture 40%, 40 ℃ of temperature, action time 6h; Carry out 80 ℃, the 20min enzyme that goes out at last.
5. improve the method for quality according to the said offal of claim 1, it is characterized in that: the compound proportion of said enzyme is: cellulase: hemicellulase: pectase: zytase=50U: 30U: 60U: 40U; Said steam blasting is that the employing saturated steam is a working media, and explosion treatment pressure is 0.8MPa, and the pressure retention time is 20 seconds; The technological parameter that complex enzyme is handled: offal moisture 45%, 45 ℃ of temperature, action time 10h; Carry out 80 ℃, the 20min enzyme that goes out at last.
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| CN2010102524130A CN101912152B (en) | 2010-08-13 | 2010-08-13 | Method for improving smoking quality by treating steam-exploded tobacco stalks with complex enzyme |
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| CN2010102524130A CN101912152B (en) | 2010-08-13 | 2010-08-13 | Method for improving smoking quality by treating steam-exploded tobacco stalks with complex enzyme |
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| CN101912152B true CN101912152B (en) | 2012-06-27 |
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| CN102599643A (en) * | 2012-03-12 | 2012-07-25 | 红云红河烟草(集团)有限责任公司 | Composite feed liquid capable of relieving ligneous gas of tobacco shreds and application of composite feed liquid |
| CN102599640B (en) * | 2012-03-13 | 2013-10-23 | 红云红河烟草(集团)有限责任公司 | Processing method for improving quality of tobacco shreds |
| CN102631021B (en) * | 2012-04-08 | 2013-11-27 | 红云红河烟草(集团)有限责任公司 | Two-stage enzyme treatment method for improving stalk shred smoking quality |
| CN102618616B (en) * | 2012-04-13 | 2013-09-11 | 吉林农业大学 | Method of improving ginsenoside extraction rate by utilizing steam-explosion combined bio-composite enzyme technology |
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| GB2069814A (en) * | 1980-02-27 | 1981-09-03 | Inst Przemyslu Fermentacyjnego | Microbiological treatment of tobacco and forming reconstituted tobacco or substitute |
| CN101427846B (en) * | 2007-11-07 | 2010-08-18 | 武汉烟草(集团)有限公司 | Method for improving efficiency of slice raw material abstraction and quality of slice flue gas with complex enzyme |
| CN101161134B (en) * | 2007-11-27 | 2012-01-04 | 北京林业大学 | Reconstructed tobacco leaf slurry, tobacco slice and method for preparing the same |
| CN101288505A (en) * | 2007-12-06 | 2008-10-22 | 云南万芳生物技术有限公司 | Method for improving quality of the tobacco leaf expanded cut stem by microorganism enzyme |
| CN101427841A (en) * | 2008-12-12 | 2009-05-13 | 华宝食用香精香料（上海）有限公司 | Method for improving quality of tobacco leaf with proteinase biotransformation |
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Address after: 610017 Jackie Chan Road, Chengdu economic and Technological Development Zone, Longquanyi District, Sichuan, China, No. 2, No. Applicant after: China Tobacco Chuanyu Industrial Co., Ltd. Address before: 610017 Jackie Chan Road, Chengdu economic and Technological Development Zone, Longquanyi District, Sichuan, China, No. 2, No. Applicant before: China Tobacco Chuanyu Industrial Corporation |
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Free format text: CORRECT: APPLICANT; FROM: CHINA TOBACCO CHUANYU INDUSTRIAL CORPORATION TO: CHINA TOBACCO CHUANYU INDUSTRIAL CO., LTD. |
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