CN102630826A - Plant extract feed additive - Google Patents
Plant extract feed additive Download PDFInfo
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- CN102630826A CN102630826A CN2012101439619A CN201210143961A CN102630826A CN 102630826 A CN102630826 A CN 102630826A CN 2012101439619 A CN2012101439619 A CN 2012101439619A CN 201210143961 A CN201210143961 A CN 201210143961A CN 102630826 A CN102630826 A CN 102630826A
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- extract
- bacillus subtilis
- perilla
- feed additive
- inokopolyose
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 20
- 239000000419 plant extract Substances 0.000 title claims abstract description 18
- 239000000284 extract Substances 0.000 claims abstract description 50
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 40
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 40
- 235000004347 Perilla Nutrition 0.000 claims abstract description 36
- 239000000843 powder Substances 0.000 claims abstract description 16
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 14
- 241000229722 Perilla <angiosperm> Species 0.000 claims description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 17
- 229960005486 vaccine Drugs 0.000 claims description 14
- 235000019441 ethanol Nutrition 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 7
- 239000000469 ethanolic extract Substances 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 239000000654 additive Substances 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 abstract description 12
- 239000003814 drug Substances 0.000 abstract description 6
- 230000003115 biocidal effect Effects 0.000 abstract description 5
- 239000003242 anti bacterial agent Substances 0.000 abstract description 3
- 229940088710 antibiotic agent Drugs 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 244000124853 Perilla frutescens Species 0.000 abstract description 2
- 150000004676 glycans Chemical class 0.000 abstract description 2
- 229920001282 polysaccharide Polymers 0.000 abstract description 2
- 239000005017 polysaccharide Substances 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract 2
- 240000000031 Achyranthes bidentata Species 0.000 abstract 1
- 235000013622 meat product Nutrition 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 239000002131 composite material Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 238000011068 loading method Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 108010059892 Cellulase Proteins 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 229940106157 cellulase Drugs 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 231100000678 Mycotoxin Toxicity 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002636 mycotoxin Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000003223 protective agent Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 241000427159 Achyranthes Species 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 244000182067 Fraxinus ornus Species 0.000 description 1
- 235000002917 Fraxinus ornus Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 235000004348 Perilla frutescens Nutrition 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000035606 childbirth Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000054 fungal extract Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a plant extract feed additive, which belongs to the field of feeds, and particularly relates to plant extract feed additives. The plant extract feed additive comprises the following components by mass: 20 to 40 percent of Achyranthes bidentata polysaccharides, 20 to 30 percent of Perilla extract, 15 to 25 percent of yeast cell wall extract and 20 to 30 percent of Bacillus subtilis freeze-dried powder, wherein Bacillus subtilis is CGMCC6012. The plant extract feed additive can effectively reduce the usage amount of antibiotic medicines in domesticated animals, improve the immunity of the domesticated animals, improve the safety of animal meat products, improve the feed utilization efficiency of animals, reduce the usage amount of antibiotics and medicines, enhance the resistance of animals, and improve the feeding benefits.
Description
Technical field:
The invention belongs to field of fodder, particularly plant extract feed additive.
Background technology:
With Inokopolyose etc. is that the green feed additive of representative becomes the developing direction of feed addictive from now on; Inokopolyose is a kind of water-soluble polysaccharide that extracts in the root of the amaranthaceous plant root of bidentate achyranthes, and good water solubility is prone to be absorbed by body, and has immunological regulation, biological function widely such as anti-oxidant, is regarded as and substitutes antibiotic natural green additive.
Feed addictive of the present invention is a primary raw material with Inokopolyose etc., and composite forming is a kind of green feed additive product of alternative antibiotics product.
Summary of the invention:
The present invention provides plant extract feed additive; Said plant extract feed additive consists of:
Inokopolyose 20-40%, extractive of perilla 20-30%, yeast cell wall extract 15-25%, bacillus subtilis freeze-dried vaccine powder 20-30%; Bacillus subtilis (Bacillus subtilis) is CGMCC6012, and aforementioned proportion is a mass percent.
Said extractive of perilla preparation method is following: perilla seed added the extraction of 7-10 times of weight absolute ethyl alcohol dipping of perilla seed after pulverizing the 40-60 mesh sieve; 30-45 ℃ of control temperature; After 1-2 hour the adjustment temperature be 55-60 ℃ 2-3 hour, extract concentrates, drying obtains ethanol extract; Add 75～85 ℃ of hot water in the perilla seed residue behind the alcohol extract, the hot water addition is 3-6 a times of perilla seed residue weight, processing time 30-50 minute, extract 2-3 time continuously, and the extract vacuum is concentrated the back spray-drying, obtain the hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, cross 60 mesh sieves, promptly get extractive of perilla.
Said Inokopolyose preparation method is following:
Radix cyathulae is pulverized the back and crosses the 30-60 mesh sieve, and interpolation radix cyathulae weight 3-6 doubly measures 70-85 ℃ of hot water and extracts 2-3 time each 30-50 minute continuously; Collect extract, in extract, add ethanol and make that concentration of alcohol reaches 70-85% in the extract, kept 2-3 hour, collecting precipitation 60-75 ℃ crushed after being dried, mistake 40 mesh sieves promptly get Inokopolyose.
Said bacillus subtilis is bacillus subtilis CGMCC6012, and number of viable is 2-8*10 in the freeze-dried vaccine powder
8Individual/g.Said plant extracts composite feed additive is made by following method:
Yeast cell wall extract was pulverized the 50-60 mesh sieve, above-mentioned crushed material and Inokopolyose and extractive of perilla and bacillus subtilis freeze-dried vaccine powder was proportionally mixed obtaining the plant extracts composite feed additive.
The present invention provides a kind of Feed Manufacturing with bacillus subtilis (Bacillus subtilis) bacterial classification; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 16th, 2012; The preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3; (postcode 100101), deposit number are CGMCC No6012.
Beneficial effect:
Yeast cell wall extract is to be raw material with the saccharomyces cerevisiae, and through the refining one type of fungal extract that forms of technologies such as breaking-wall cell, enzymolysis, separation purification and drying, finished product is generally light grey powder to Dark grey.Research confirms that yeast cell wall is divided into 3 layers: outer is manna oligosacchride and protein conjugate, and the intermediate layer is β-(1,3), β-(1,6) glucan, and internal layer is a chitin.Its special configuration can form special complementary structure with multiple mycotoxin, thereby combines firmly with multiple mycotoxin, and discharges outside the animal body through enteron aisle.
The medicinal plant purple perilla is the band leaf spray of labiate purple perilla [Perilla frutescens (L.) Brltt.var.arguta Benth.Hand.-Mazz.].Be used as medicine so that stem, leaf and son are real.Purple perilla belongs to comprehensive intersection commodity, and is both pharmaceutically acceptable, is clinical medicine commonly used, can eat again.The form of being used as medicine is claimed lac encrusted twig with stem, and leaf is claimed Su Ye again, induces sweat, and son is claimed perillaseed, FRUCTUS PERILLAE, red perillaseed again, is the important component of Suzi Jiangqi Tang.Expelling cold and relieving exterior syndrome, regulating the flow of QI to ease the stomach.Cure mainly cold, fever, be afraid of cold, lossless, uncomfortable in chest, the stomachache that crab poisons and causes is separated in cough, diarrhoea, diseases such as vomiting.
Bacillus subtilis: have following function: 1. oxygen consumption produces acid, regulates the intestinal microecology balance, reaches diseases such as prevention diarrhoea, diarrhea; 2. secrete multiple enzyme, improve the feed digestive utilization ratio; 3. generation various nutrients is participated in body metabolism; 4. raising animal immunizing power.
Perilla seed of the present invention carries out pulverize and break cellular wall to be handled; Make function factor realization full price stripping and high efficiency extraction utilization in the perilla seed; Adopt organic solvent and hot water to realize wherein effective extraction of different efficacies composition respectively; Particularly control the temperature section extraction and effectively improved extract yield especially, improved the extraction efficiency and the quality of the extract of perilla seed.
The present invention is composite through science, and bacillus subtilis, purple perilla, Inokopolyose have been realized organic assembling, and product of the present invention can effectively reduce the usage quantity of antibiotics in letting animals feed; Improve the immunity of letting animals feed; Improve the security of animal flesh goods, improve the food utilization efficiency of animal, the use of antibiotic and medicine in the conventional raising of minimizing; Strengthen the resistivity of animal, improve raise benefit.
The practical implementation method:
Following embodiment can make those skilled in the art more fully understand the present invention, but does not limit the present invention in any way.Bacterial screening method:
Induction mutation of bacterium
Take the ultraviolet mutagenesis method: with CICC10023 is starting strain, processes bacteria suspension, coats on the culture medium plate, cultivates in 37 ℃ of incubators behind the ultraviolet irradiation 90s.
Bacterial screening
Choose 30 single bacterium colonies of looking strong from the 90s plate, be inoculated on the inclined-plane, in 37 ℃ of incubators, cultivated 0--4 ℃ of stored refrigerated 24 hours; With original strain as contrast; Adopt conventional seed selection condition CMC as carbon source and proteolysis circle method screening cellulase-producing and the strong bacterial strain of protease; Screening obtains cellulase-producing and strong bacterial strain bacillus subtilis (Bacillus subtilis) L1 of protease; This bacterial strain cellulase-producing can improve 25% by the force rate original strain, produces protease and can improve 52% by the force rate original strain.In the common micro-organisms center preservation of Chinese common micro-organisms culture presevation administration committee, deposit number is CGMCC No 6012 with this bacterial classification.
Culture medium is formed: peptone 5 grams; Beef extract 3 grams; Sodium chloride 5 grams; Agar 20 grams; 1000 milliliters of distilled water; PH7.0." yeast cell wall extract " is available from Hai Site group company or other companies among the present invention.
Embodiment 1:
10 kilograms of feed addictives consist of:
Inokopolyose 30%, extractive of perilla 25%, yeast cell wall extract 20%, bacillus subtilis freeze-dried vaccine powder 25%, bacillus subtilis is CGMCC6012, aforementioned proportion is a mass percent.
Said extractive of perilla preparation method is following: perilla seed is pulverized and is added 8 times of weight absolute ethyl alcohols dippings of perilla seed behind 50 mesh sieves and extract, 40 ℃ of temperature 2 hours, and back adjustment temperature is 2 hours 58 ℃ of times, the extract vacuum concentrates, drying obtains ethanol extract; Add 80 ℃ of hot water in the perilla seed residue behind the alcohol extract, the hot water addition is 5 times of perilla seed residue weight, 40 minutes processing times, extract continuously 2 times, and the extract vacuum is concentrated the back spray-drying, obtain the hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, cross 60 mesh sieves, promptly get extractive of perilla;
Said Inokopolyose preparation method is following:
Radix cyathulae is pulverized the back and crosses 40 mesh sieves, adds 80 ℃ of hot water of 4 times of amounts of radix cyathulae weight and extracts 2 times each 40 minutes continuously; Collect extract, in extract, add ethanol and make that concentration of alcohol reaches 75% in the extract, the control temperature kept 2 hours at 25 ℃, and 75 ℃ of crushed after being dried of collecting precipitation are crossed 40 mesh sieves, promptly get Inokopolyose;
Said bacillus subtilis is that bacillus subtilis is a CGMCC6012 freeze-dried vaccine powder, and number of viable is 5*10
8Individual/g;
Said plant extracts composite feed additive is made by following method:
Yeast cell wall extract was pulverized 60 mesh sieves, with above-mentioned crushed material and Inokopolyose and extractive of perilla and bacillus subtilis freeze-dried vaccine powder proportionally mixed obtain the plant extracts composite feed additive.
The used bacillus subtilis of this product (Bacillus subtilis) bacterial classification; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 16th, 2012; The preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3; (postcode 100101), deposit number are CGMCC No6012.
The preparation of bacillus subtilis microbial agent:
1. the acquisition of zymotic fluid: adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: bacillus subtilis CGMCC6012 slant strains is inserted in 500 ml shake flasks 100 milliliters of culture medium loading amounts, 180 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: first order seed is inserted 500 milliliters of secondary seeds according to 10% inoculum concentration shake in the bottle, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is inserted 5000 milliliters of three grades of seeds with 10% inoculum concentration shake in the bottle 1000 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(4) first class seed pot is cultivated: it is the first class seed pot of 150L that three grades of seeds are inserted total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 100L, 28 ℃ of cultivation temperature; 100 rev/mins of mixing speeds; Ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed jars that the first class seed pot bacterial classification is inserted total measurement (volume) with 10% inoculum concentration; 1 ton of fermentation medium loading amount, 28 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds; Ventilation (V/V) 1:0.5; Tank pressure 0.05Mpa, incubation time 24 hours is cultivated and is finished bacteria concentration 11 * 10
8Individual/ml.
(6) centrifugation: adopt centrifuge to separate and obtain thalline.
(7) vacuum freeze drying: adopt drying process with atomizing to handle the acquisition dry bacteria after adding protective agent; Protective agent consists of defatted milk 10%, lactose 5%, glycerine 5%.Vacuum freeze-drying technique is write the culture presevation handbook referring to Institute of Microorganism, Academia Sinica, publishes in 1980. 610-653.
Culture medium is formed: peptone 5 grams; Beef extract 3 grams; Sodium chloride 5 grams; Agar 20 grams; 1000 milliliters of distilled water; PH7.0.
The product result of use
The test of the present invention's example 1 feed addictive result of use
Test method:
Select Dongguan City twins feed corporation,Ltd subordinate pig farm; Select each 50 of age in days, body weight close healthy sow, piglet and boars; All be divided into 2 groups (test group and control groups) at random, test group is used product of the present invention, and control group does not add product of the present invention; The addition of product of the present invention is the 1-2% of feed consumption, feeds continuously 60 days.Compare with control group, use the invention feed addictive can obtain following effect:
(1) number born of sow on average increases by 0.7;
(2) the boar sperm count increases by 20%～25%, improves conception rate;
(3) before and after the childbirth, the prevention of sow constipation number of times reduces more than 34%;
(4) the weanling pig weight average increases to 6.3kg from 5.4kg, increases by 11% than control group weight average;
(5) the antibiotic use amount has reduced 73-85% the piglet phase;
The above results shows that product of the present invention can improve sow, piglet and boar body immunity, reduces antibiotic dosage, increases to culture quality and benefits.
Embodiment 2: implementation method is basic with example 1;
10 kilograms of feed addictives consist of:
Inokopolyose 20%, extractive of perilla 30%, yeast cell wall extract 20%, bacillus subtilis freeze-dried vaccine powder 30%, bacillus subtilis is CGMCC6012, aforementioned proportion is a mass percent.
Embodiment 3: implementation method is basic with example 1;
10 kilograms of feed addictives consist of:
Inokopolyose 40%, extractive of perilla 20%, yeast cell wall extract 17%, bacillus subtilis freeze-dried vaccine powder 23%, bacillus subtilis is CGMCC6012, aforementioned proportion is a mass percent.
Embodiment 4: implementation method is basic with example 1;
10 kilograms of feed addictives consist of:
Inokopolyose 28%, extractive of perilla 22%, yeast cell wall extract 21%, bacillus subtilis freeze-dried vaccine powder 29%, bacillus subtilis is CGMCC6012, aforementioned proportion is a mass percent.
Claims (5)
1. plant extract feed additive; It is characterized in that consisting of: Inokopolyose 20-40%, extractive of perilla 20-30%, yeast cell wall extract 15-25%; Bacillus subtilis (Bacillus subtilis) freeze-dried vaccine powder 20-30%; Bacillus subtilis (Bacillus subtilis) is CGMCC6012, and aforementioned proportion is a mass percent, and said extractive of perilla preparation method is following: perilla seed added the extraction of 7-10 times of weight absolute ethyl alcohol dipping of perilla seed after pulverizing the 40-60 mesh sieve; Temperature 30-45 ℃ of temperature of adjustment after 1-2 hour be 55-60 ℃ 2-3 hour, extract concentrates, drying obtains ethanol extract; Add 75～85 ℃ of hot water in the perilla seed residue behind the alcohol extract, the hot water addition is 3-6 a times of perilla seed residue weight, processing time 30-50 minute, extract 2-3 time continuously, and the extract vacuum is concentrated the back spray-drying, obtain the hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, cross 60 mesh sieves, promptly get extractive of perilla; Said Inokopolyose preparation method pulverizes the back for radix cyathulae and crosses the 30-60 mesh sieve, and interpolation radix cyathulae weight 3-6 doubly measures 70-85 ℃ of hot water and extracts 2-3 time each 30-50 minute continuously; Collect extract, in extract, add ethanol and make that concentration of alcohol reaches 70-85% in the extract, kept 2-3 hour, collecting precipitation 60-75 ℃ crushed after being dried, mistake 40 mesh sieves promptly get Inokopolyose.
2. according to the said plant extract feed additive of claim 1; It is characterized in that said bacillus subtilis CGMCC6012 freeze-dried vaccine powder number of viable is 2-8*10
8Individual/g.
3. according to the said plant extract feed additive of claim 2; It is characterized in that said bacillus subtilis CGMCC6012 freeze-dried vaccine powder number of viable is 5*10
8Individual/g.
4. according to the said plant extract feed additive of claim 1; It is characterized in that additive consists of: Inokopolyose 28%, extractive of perilla 22%, yeast cell wall extract 21%, bacillus subtilis freeze-dried vaccine powder 29%, bacillus subtilis is CGMCC6012.
5. according to the said plant extract feed additive of claim 1; It is characterized in that additive consists of: Inokopolyose 40%, extractive of perilla 20%, yeast cell wall extract 17%, bacillus subtilis freeze-dried vaccine powder 23%, bacillus subtilis is CGMCC6012.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101439619A CN102630826B (en) | 2012-05-09 | 2012-05-09 | Plant extract feed additive |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101439619A CN102630826B (en) | 2012-05-09 | 2012-05-09 | Plant extract feed additive |
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| Publication Number | Publication Date |
|---|---|
| CN102630826A true CN102630826A (en) | 2012-08-15 |
| CN102630826B CN102630826B (en) | 2013-07-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101439619A Active CN102630826B (en) | 2012-05-09 | 2012-05-09 | Plant extract feed additive |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734477A (en) * | 2013-12-28 | 2014-04-23 | 邵素英 | Preparation method of efficient feed additive |
| CN103749977A (en) * | 2013-12-28 | 2014-04-30 | 邵素英 | Efficient feed additive |
| CN104719632A (en) * | 2013-12-19 | 2015-06-24 | 天津工业大学 | Feed additive and preparation method thereof |
| CN107319103A (en) * | 2017-08-01 | 2017-11-07 | 河南牧业经济学院 | Promote feed addictive of growth of animals or poultry and intestinal growth and its preparation method and application |
| CN109287872A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For improving the additive and its preparation method and application of poultry immunity of organisms |
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|---|---|---|---|---|
| CN1961710A (en) * | 2006-12-01 | 2007-05-16 | 马永旺 | High-quality beef cattle bred by quick fermentation of Chinese herbs |
| CN101536730A (en) * | 2007-04-04 | 2009-09-23 | 何执中 | Application of traditional Chinese medicine formula in shrimp farming |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719632A (en) * | 2013-12-19 | 2015-06-24 | 天津工业大学 | Feed additive and preparation method thereof |
| CN103734477A (en) * | 2013-12-28 | 2014-04-23 | 邵素英 | Preparation method of efficient feed additive |
| CN103749977A (en) * | 2013-12-28 | 2014-04-30 | 邵素英 | Efficient feed additive |
| CN107319103A (en) * | 2017-08-01 | 2017-11-07 | 河南牧业经济学院 | Promote feed addictive of growth of animals or poultry and intestinal growth and its preparation method and application |
| CN109287872A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For improving the additive and its preparation method and application of poultry immunity of organisms |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102630826B (en) | 2013-07-31 |
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