CN102618480B - Preparation method of dry algae powder of anabaena azollae - Google Patents
Preparation method of dry algae powder of anabaena azollae Download PDFInfo
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- CN102618480B CN102618480B CN 201210103503 CN201210103503A CN102618480B CN 102618480 B CN102618480 B CN 102618480B CN 201210103503 CN201210103503 CN 201210103503 CN 201210103503 A CN201210103503 A CN 201210103503A CN 102618480 B CN102618480 B CN 102618480B
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Abstract
The invention relates to a preparation method of dry algae powder of anabaena azollae. The preparation method comprises the following steps of: using a basic nutrient solution containing dipotassium hydrogen phosphate, ironic citrate, molybdic acid and calcium carbonate with the pH value of 8, and culturing the anabaena azollae for 2-3 days on the basis of adding potassium nitrate to realize the rapid activation of the anabaena azollae; transferring the activated anabaena azollae to a non-transparent open container, supplementing the basic nutrient solution, adding glucose, and culturing for 5-7 days again; and dumping the basic nutrient solution, transferring the cultured anabaena azollae to a porcelain dish covered by a plastic film, drying and smashing at a certain condition and obtaining lots of dry algae powder of anabaena azollae. According to the preparation method, the germinating proportion of the dry algae powder is increased, the germinating time is shortened, the stress resistance of the anabaena azollae is increased, and the dry algae powder can be more efficiently obtained; and the preparation method has the advantages of simple technology, easiness in popularization and implementation, safe and efficient fertilizers and non-pollution on environments and foods, and therefore, the preparation method has enormous theoretical value and application value.
Description
Technical field
The present invention relates to a kind of preparation method of algae powder, be specifically related to a kind of preparation method of Anabaena Azollae Based dry algae powder of special existence, belong to biological technical field.
Background technology
Anabaena Azollae Based (Anabaena azollae) provides (numbering: FACHB-190) by Inst. of Hydrobiology, Chinese Academy of Sciences's algae kind storehouse, and at " Jianying Shen, Antonio DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li; Molecular basis for differential metabolic responses to monosulfuron in three nitrogen-fixing cyanobacteria, Weed Science, 2009,57:178-188 ", " Jianying Shen, Jing Jiang, Peizhong Zheng; Effects of Light and Monosulfuron on growth and Photosynthetic Pigments of Anabaenaflos-aquae Breb, Water Resource and Protection, 2009,1:408-413 " upper open.
Anabaena Azollae Based is a kind of blue-green algae that colonizes in the Herba azollae imbricatae plant materials, the fixing nomadic nitrogen in atmosphere, its distinctive algae somatotropin can promote that crop grows vigorously, and is remarkable to the increasing production of rice effect, generally reach 1-2, and the effect of improvement soil and inhibition weeds is arranged.The Anabaena Azollae Based individuality is little, breeding is fast, be convenient to cultured continuously, it is a kind of good biological source of manure, due to the production of agrochemicals fertilizer and agricultural chemicals and use and to bring a large amount of greenhouse gas emissions, therefore developing agro-ecology fixed nitrogen technology, reduce applying quantity of chemical fertilizer, is the important channel of current development low-carbon (LC) agricultural.Along with the fast development of agricultural science and technology, bio-bacterial manure will more and more be exploited.
The purposes of Anabaena Azollae Based mainly contains: as the good material of fixed nitrogen and symbiosis research, be used for extracting natural pigment, algae is short says growth hormone, phycobiliprotein and polysaccharide, is used as green manure, exploitation hydrogen energy source etc.
At present both at home and abroad to the correlative study of Anabaena Azollae Based dry algae powder also seldom, the Chinese Academy of Sciences once mentioned the induction method of high spore content in " the Absorption and fluorescence spectrum characteristic of Anabaena Azollae Based dormancy and germination spore ", aspect the preparation of algae dry algae powder, rarely seen Shen Yin is military to be waited mention a large amount of productions and air-dry or the method such as dry in " result and prospect that Azotica is cultivated and used ", and exploration in other respects is rare report.
Summary of the invention
The object of the invention is to overcome the deficiency that prior art exists, a kind of preparation method of Anabaena Azollae Based dry algae powder is provided, take micro-Anabaena Azollae Based dry algae powder as raw material, adding physico chemical factor efficiently activates, and make the breeding of Anabaena Azollae Based Fast Growth by suitable conditioned stimulus, relend and help plastic film and special drying mode, produce the Anabaena Azollae Based dry algae powder that to have stronger resistance and higher vigor in enormous quantities, the method has safety and characteristics easily.
The present invention is achieved by the following technical solutions:
A kind of preparation method of Anabaena Azollae Based dry algae powder, the pH value that its use contains dipotassium hydrogen phosphate, ironic citrate, molybdic acid and calcium carbonate is 8 basic culture solution, cultivate Anabaena Azollae Based 2-3 days on the basis of adding saltpetre, realize the quick active of Anabaena Azollae Based; The Anabaena Azollae Based that activates is transferred in opaque open container, replenished basic culture solution and add glucose, then cultivated 5-7 days; Outwell basic culture solution, and the Anabaena Azollae Based of cultivating is transferred in the porcelain dish that is coated with plastic film, dry and pulverizing, gather in the crops Anabaena Azollae Based dry algae powder in enormous quantities under certain condition.
Cultivate Anabaena Azollae Based on described basis adding saltpetre referred in 2-3 days: the Anabaena Azollae Based dry algae powder that takes trace, be dissolved in basic culture solution and smash, again algae liquid is transferred in culture dish, add saltpetre and algae liquid pH value is transferred to after 8, being placed in sterilisable chamber the timing agitation cultivation 2-3 days.
The diameter of described culture dish is 90mm.
Described interpolation saltpetre makes its concentration reach 250mg/L or 380mg/L.
The light intensity of described sterilisable chamber is that 3000 ± 200Lx, temperature are 30 ± 2 ℃.
Described timing refers to: every day is at the same time.
Described stirring refers to: break up cell even by the triangle rod of the bacterium of going out or the liquid-transfering gun of 5ml.
The component of described basic culture solution is: 280mg/L dipotassium hydrogen phosphate, 10mg/L ironic citrate, 10mg/L molybdic acid, 100mg/L calcium carbonate, surplus is life tap water used.
The height of described open container is 20-50cm.
The described glucose that adds makes its concentration reach 380-500mg/L.
The height of described porcelain dish is 10-20cm.
Described dry and pulverizing under certain condition refers to: the illumination dry 3-5 in chamber days of 30 ℃ are dried or be placed in to the baking oven that the described porcelain dish that fills the Anabaena Azollae Based of cultivation is placed in 35-40 ℃, and pulverize with pulverizer.
The preparation method of Anabaena Azollae Based dry algae powder of the present invention is take micro-Anabaena Azollae Based dry algae powder as raw material, by quick active-three of cultivation breeding-drying and crushing technical process, produce the Anabaena Azollae Based dry algae powder that to have stronger resistance in enormous quantities.
The contriver has carried out great many of experiments, find out the optimal procedure parameters in described preparation method, see also Fig. 1,2 and 3, optimised process is as follows: take dipotassium hydrogen phosphate 75mg, ironic citrate 5mg, molybdic acid 2.5mg and calcium carbonate 100mg and be dissolved in 1L water, make basic culture solution, add saltpetre 300mg, cultivate Anabaena Azollae Based after 3 days, add again glucose 400mg, cultured continuously 6 days; Outwell nutrient solution, and Anabaena Azollae Based is transferred in the porcelain dish that is coated with plastic film, dry in the baking oven of 38 ℃, after crushed, namely gather in the crops the Anabaena Azollae Based dry algae powder of volume production.
Beneficial effect of the present invention is: effectively activation condition has improved the sprouting ratio of dry algae powder greatly, has shortened sprout time; The use of glucose has strengthened the resistance of Anabaena Azollae Based; Drying mode by plastic film makes the results of dry algae powder more efficient.The preparation method of described Anabaena Azollae Based dry algae powder have technique simple, be easy to promotion and implementation, fertilizer is safe and efficient, can not cause the new advantages such as pollution to environment and food safety after using, so the present invention has great theory value and using value.
Description of drawings
Fig. 1 is under the activation of different concns dipotassium hydrogen phosphate, the content of Anabaena Azollae Based in cultured continuously 7 days.
Fig. 2 is under the activation of different concns saltpetre, the content of Anabaena Azollae Based in cultured continuously 7 days.
Fig. 3 is under the effect of different glucose, the content of Anabaena Azollae Based in cultured continuously 7 days.
Fig. 4 is preparation technology's flow process of the present invention.
Embodiment
See also Fig. 4, the preparation method of Anabaena Azollae Based dry algae powder of the present invention is take micro-Anabaena Azollae Based dry algae powder as raw material, by quick active-three of cultivation breeding-drying and crushing technical process, produce the Anabaena Azollae Based dry algae powder that to have stronger resistance in enormous quantities.
Main technological steps of the present invention is: using the pH value contain dipotassium hydrogen phosphate, ironic citrate, molybdic acid and calcium carbonate is 8 basic culture solution, and the cultivation Anabaena Azollae Based is 2-3 days on the basis of adding saltpetre, realizes the quick active of Anabaena Azollae Based; The Anabaena Azollae Based that activates is transferred in opaque open container, replenished basic culture solution and add glucose, then cultivated 5-7 days; Outwell basic culture solution, and the Anabaena Azollae Based of cultivating is transferred in the porcelain dish that is coated with plastic film, dry and pulverizing, gather in the crops Anabaena Azollae Based dry algae powder in enormous quantities under certain condition.
Below in conjunction with embodiment, the present invention is elaborated, this embodiment has provided detailed embodiment and concrete operating process take technical solution of the present invention as prerequisite, but protection scope of the present invention is not limited only to following embodiment.
Main raw and equipment: saltpetre, sodium hydroxide, hydrochloric acid, glucose, basic culture solution, Anabaena Azollae Based dry algae powder, beat algae machine, culture dish, open container, plastic film, porcelain dish, sterilisable chamber, illumination chamber.
Concrete scheme is: the Anabaena Azollae Based dry algae powder that takes 0.01g, be dissolved in the 10mL basic culture solution in small beaker, smash 3 times with the algae machine of beating after 75% alcohol sterilization, each 10 seconds, the component of this basic culture solution is: 280mg/L dipotassium hydrogen phosphate, 10mg/L ironic citrate, 10mg/L molybdic acid, 100mg/L calcium carbonate, and surplus is life tap water used; Again algae liquid is transferred in the culture dish of Φ 90 * 15mm, rinsed that small beaker is poured in culture dish in order to avoid residual with basic culture solution, with adding basic culture solution to 35mL in backward culture dish again; Then add saltpetre, make its concentration reach 250mg/L, with sodium hydroxide and hydrochloric acid, algae liquid pH value is transferred to 8, afterwards culture dish is placed on light intensity and is 3000 ± 200Lx, temperature and be in the sterilisable chamber of 30 ± 2 ℃ Anabaena Azollae Based is cultivated, the while timing agitation, being every day is breaing up cell by the liquid-transfering gun of the triangle rod of the bacterium of going out or 5ml evenly at the same time, cultivates generally that after 2-3 days, Anabaena Azollae Based is activated in a large number.Transfer to the Anabaena Azollae Based that activates in opaque open container this moment, and the height of this open container is 20-50cm, and length and width are unrestricted; Then replenish appropriate basic culture solution, take glucose and be added in open container, make its concentration reach 380mg/L, then cultivated 5-7 days.Pour out afterwards unnecessary basic culture solution, it is in the porcelain dish of 10-20cm that a large amount of Anabaena Azollae Baseds of cultivating are transferred to the height that is coated with plastic film, and it is indoor that this porcelain dish is placed in the illumination of 30 ℃, after dry 3-5 days Anabaena Azollae Based automatically and plastic film peel off, pulverize dried Anabaena Azollae Based with pulverizer again, thereby gather in the crops large batch of Anabaena Azollae Based dry algae powder.
Main raw and equipment: saltpetre, sodium hydroxide, hydrochloric acid, glucose, basic culture solution, Anabaena Azollae Based dry algae powder, beat algae machine, culture dish, open container, plastic film, porcelain dish, sterilisable chamber, illumination chamber.
The concrete scheme of the present embodiment is except following processing condition, and all the other contents and step process are identical with above-described embodiment 1:
Add saltpetre, make its concentration reach 380mg/L;
Take glucose and be added in open container, make its concentration reach 450mg/L.
Main raw and equipment: saltpetre, sodium hydroxide, hydrochloric acid, glucose, basic culture solution, Anabaena Azollae Based dry algae powder, play algae machine, culture dish, open container, plastic film, porcelain dish, sterilisable chamber, baking oven.
Concrete scheme is: the Anabaena Azollae Based dry algae powder that takes 0.01g, be dissolved in the 10mL basic culture solution in small beaker, smash 3 times with the algae machine of beating after 75% alcohol sterilization, each 10 seconds, the component of this basic culture solution is: 280mg/L dipotassium hydrogen phosphate, 10mg/L ironic citrate, 10mg/L molybdic acid, 100mg/L calcium carbonate, and surplus is life tap water used; Again algae liquid is transferred in the culture dish of Φ 90 * 15mm, rinsed that small beaker is poured in culture dish in order to avoid residual with basic culture solution, with adding basic culture solution to 35mL in backward culture dish again; Then add saltpetre, make its concentration reach 380mg/L, with sodium hydroxide and hydrochloric acid, algae liquid pH value is transferred to 8, afterwards culture dish is placed on light intensity and is 3000 ± 200Lx, temperature and be in the sterilisable chamber of 30 ± 2 ℃ Anabaena Azollae Based is cultivated, the while timing agitation, being every day is breaing up cell by the liquid-transfering gun of the triangle rod of the bacterium of going out or 5ml evenly at the same time, cultivates generally that after 2-3 days, Anabaena Azollae Based is activated in a large number.Transfer to the Anabaena Azollae Based that activates in opaque open container this moment, and the height of this open container is 20-50cm, and length and width are unrestricted; Then replenish appropriate basic culture solution, take glucose and be added in open container, make its concentration reach 380mg/L, then cultivated 5-7 days.Pour out afterwards unnecessary basic culture solution, it is in the porcelain dish of 10-20cm that a large amount of Anabaena Azollae Baseds of cultivating are transferred to the height that is coated with plastic film, and this porcelain dish is placed in the baking oven of 40 ℃, after dry 12 hours Anabaena Azollae Based automatically and plastic film peel off, pulverize dried Anabaena Azollae Based with pulverizer again, thereby gather in the crops large batch of Anabaena Azollae Based dry algae powder.
Main raw and equipment: saltpetre, sodium hydroxide, hydrochloric acid, glucose, basic culture solution, Anabaena Azollae Based dry algae powder, beat algae machine, culture dish, open container, plastic film, porcelain dish, sterilisable chamber, illumination chamber.
The concrete scheme of the present embodiment is except following processing condition, and all the other contents and step process are identical with above-described embodiment 3:
Add saltpetre, make its concentration reach 250mg/L;
Take glucose and be added in open container, make its concentration reach 500mg/L;
Porcelain dish is placed in the baking oven of 35 ℃.
Above embodiment is not limitation of the present invention, can prepare by a certain percentage the nutrient solution of large volume when specifically implementing, and as adding 35-50mg glucose in the 100mL basic culture solution, adds 0.03g Anabaena Azollae Based dry algae powder; Add 350-500mg glucose in the 1L basic culture solution, add 0.30g Anabaena Azollae Based dry algae powder, culture dish diameter 150mm etc.
Claims (9)
1. the preparation method of an Anabaena Azollae Based dry algae powder, it is characterized in that, the pH value that use contains dipotassium hydrogen phosphate, ironic citrate, molybdic acid and calcium carbonate is 8 basic culture solution, take the Anabaena Azollae Based dry algae powder of 0.01g, be dissolved in basic culture solution and smash, again algae liquid is transferred in culture dish, add saltpetre and algae liquid pH value is transferred to after 8, being placed in sterilisable chamber timing agitation cultivated 2-3 days, the light intensity of described sterilisable chamber is that 3000 ± 200Lx, temperature are 30 ± 2 ℃, realizes the quick active of Anabaena Azollae Based; The Anabaena Azollae Based that activates is transferred in opaque open container, replenished basic culture solution and add glucose, then cultivated 5-7 days; Outwell basic culture solution, and the Anabaena Azollae Based of cultivating is transferred in the porcelain dish that is coated with plastic film, the described porcelain dish that fills Anabaena Azollae Based is placed in the baking oven oven dry of 35-40 ℃ or is placed in 30 ℃ the illumination dry 3-5 in chamber days, and pulverize dried Anabaena Azollae Based with pulverizer, gather in the crops Anabaena Azollae Based dry algae powder in enormous quantities.
2. the preparation method of Anabaena Azollae Based dry algae powder according to claim 1, is characterized in that, the diameter of described culture dish is 90mm.
3. the preparation method of Anabaena Azollae Based dry algae powder according to claim 1, is characterized in that, described interpolation saltpetre makes its concentration reach 250mg/L or 380mg/L.
4. the preparation method of Anabaena Azollae Based dry algae powder according to claim 1, is characterized in that, described timing refers to: every day is at the same time.
5. the preparation method of Anabaena Azollae Based dry algae powder according to claim 1, is characterized in that, described stirring refers to: break up cell even by the triangle rod of the bacterium of going out or the liquid-transfering gun of 5ml.
6. the preparation method of the described Anabaena Azollae Based dry algae powder of according to claim 1 to 5 any one, it is characterized in that, the component of described basic culture solution is: 280mg/L dipotassium hydrogen phosphate, 10mg/L ironic citrate, 10mg/L molybdic acid, 100mg/L calcium carbonate, surplus is life tap water used.
7. the preparation method of Anabaena Azollae Based dry algae powder according to claim 1, is characterized in that, the height of described open container is 20-50cm.
8. the preparation method of Anabaena Azollae Based dry algae powder according to claim 1, is characterized in that, the described glucose that adds makes its concentration reach 380-500mg/L.
9. the preparation method of Anabaena Azollae Based dry algae powder according to claim 1, is characterized in that, the height of described porcelain dish is 10-20cm.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242078A (en) * | 2011-02-25 | 2011-11-16 | 上海交通大学 | Induction technology of anabaena flosaquae chlamydospore and preparation method of dry algae powder thereof |
| CN102628027A (en) * | 2012-04-09 | 2012-08-08 | 上海交通大学 | Method for rapidly activating and abundantly producing anabaena azotica ley dry powder |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242078A (en) * | 2011-02-25 | 2011-11-16 | 上海交通大学 | Induction technology of anabaena flosaquae chlamydospore and preparation method of dry algae powder thereof |
| CN102628027A (en) * | 2012-04-09 | 2012-08-08 | 上海交通大学 | Method for rapidly activating and abundantly producing anabaena azotica ley dry powder |
Non-Patent Citations (4)
| Title |
|---|
| Effects of Light and Monosulfuron on Growth and Photosynthetic Pigments of Anabaena Flos-Aquae Breb;Jianying SHEN等;《J.Water Resource and Protection》;20091231;408-413 * |
| Jianying SHEN等.Effects of Light and Monosulfuron on Growth and Photosynthetic Pigments of Anabaena Flos-Aquae Breb.《J.Water Resource and Protection》.2009, |
| 固氮蓝藻培养和应用的结果与展望;沈银武 等;《水生生物学报》;19931231;第17卷(第4期);357-364 * |
| 沈银武 等.固氮蓝藻培养和应用的结果与展望.《水生生物学报》.1993,第17卷(第4期), |
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