CN102898208A - Composite manure water preparation - Google Patents
Composite manure water preparation Download PDFInfo
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- CN102898208A CN102898208A CN2012103419959A CN201210341995A CN102898208A CN 102898208 A CN102898208 A CN 102898208A CN 2012103419959 A CN2012103419959 A CN 2012103419959A CN 201210341995 A CN201210341995 A CN 201210341995A CN 102898208 A CN102898208 A CN 102898208A
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- ions
- rich water
- water preparation
- composite
- acid bacteria
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Abstract
The invention relates to a composite manure water preparation which comprises the following substances by weight percent: 80 to 92 % of decomposed chicken manure, 3 to 10 % of calcium superphosphate, 1 to 5 % of potassium dihydrogen phosphate, 0.5 to 5 % of calcium carbonate, 0.02 to 0.35 % of zinc ions, 0.04 to 0.70 % of manganese ions, 0.1 to 0.6 % of copper ions, 0.1 to 0.7 % of divalent iron ions, 0.02 to 0.2 % of molybdenum ions, 0.02 to 0.35% of the boron ions and 0.5 to 1 % of nitzschia. In addition, the composite manure water preparation provided by the invention is added with bacterial liquid of Bacillus subtilis, lactic acid bacteria and bifidobacteria, so that the numbers of the Bacillus subtilis, lactic acid bacteria and bifidobacteria in the composite preparation are respectively 10<6> to 10<8> CFU/kg. According to the invention, by using the fermented chicken manure as main raw materials, together with N, P, K, trace elements, etc. necessary for phytoplankton and zooplankton, the prepared organic manure water preparation is sufficient in organic nutrients and trace element nutrients and reasonable in compatibility, and can quickly supplement small molecular organic debris needed by pearl mussel, and N, P, K and a variety of trace elements needed by the phytoplankton and the zooplankton.
Description
Technical field
The invention belongs to the agriculture production product technique field, be specifically related to a kind of rich water compound formulation, be i.e. a kind of rich water compound formulation of suitable pearl culturing clam cultivation waters use.
Background technology
At present, the rich water product of culture fishery is a lot, but mostly configures for fishery products such as fishes and shrimps.And the pearl freshwater mussel, as a kind of large-scale economic benthic mollusca, its cultivation requires with general breed variety different.Present rich water product does not satisfy the pearl freshwater mussel to the demand of the basic nutritive elements such as nitrogen, phosphorus, potassium, calcium.And, also have a lot of mineral elements and trace element also very important at the foster freshwater mussel fishing waters of growing cultured pearls, growth and being formed with directly and indirectly of pearl of pearl culturing clam affected.For example, nitrogen, phosphorus, potassium are the necessary elements of phytoplankton growth and breeding, and plant plankton is the primary productivity of water body, and plankton and organic debris are the basic substances that pearl culturing clam is depended on for existence.Food organism are abundant in water, the nutritional sufficiency of pearl culturing clam, and pearl also just looks soon, and quality is high.It is different that this and general aquaculture need to be thrown in bait.In addition, the specific plant plankton of dwelling property of the end such as diatom also has significant promoter action to the growth of pearl culturing clam.Simultaneously, consider that calcium (existing with calcium carbonate form) is the major ingredient of pearl culturing clam shell and pearl, therefore, the calcium contents that the aquaculture water of pearl culturing clam requires is higher than ortho-water product demand; Pearl culturing clam all requires certain content for copper, zinc, manganese, iron etc.Therefore exploitation has very great meaning for the rich water preparation of pearl culturing clam aquaculture water special use.
Summary of the invention
The purpose of this invention is to provide the rich water compound formulation that use in a kind of suitable pearl culturing clam cultivation waters, have that calcic is high, nutritious, fertilizer efficiency is both lasting rapidly, can go out plant plankton and zooplankton by high effect culture.
Rich water compound formulation of the present invention, include the material of following weight percent: Chicken dung 80～92%, calcium superphosphate 3～10%, potassium primary phosphate 1～5%, calcium carbonate 0.5～5%, zine ion 0.02～0.35%, mn ion 0.04～0.70%, cupric ion 0.1～0.6%, ferrous ion 0.1～0.7%, molybdenum ion 0.02～0.2%, boron ion 0.02～0.35%, rhombus algae 0.5～1%.
Rich water compound formulation of the present invention also is added with the bacterium liquid of genus bacillus, milk-acid bacteria and bifidus bacillus, makes genus bacillus, milk-acid bacteria and the bifidus bacillus quantity in compound formulation be respectively 10
6～10
8CFU/kg.
The present invention utilizes chicken manure after the fermentation as main raw material, cooperates plant plankton and the necessary N of zooplankton, P, K and trace element etc., the fertilizer water preparation of making, and organic nutrient and micronutrient are sufficient, cooperate rationally.Use the advantage of rich water compound formulation of the present invention as follows:
(1) the present invention can replenish the required small molecules organic debris of pearl culturing clam and plankton needs N, P, K and various trace elements fast.
(2) the present invention can directive breeding the pearl culturing clam end the dwelling property planktonic organisms such as needed diatom that grow.
(3) part material of the present invention is taken from the waste material that poultry husbandry produces, and turns waste into wealth, and has avoided a large amount of wastings of resources, and preparation is simple and easy to use.
Embodiment
The preparation method of Chicken dung adopts traditional method of naturally becoming thoroughly decomposed, and becomes thoroughly decomposed after chicken manure is piled up, and stirs therebetween, and to the chicken manure odorless, the brown that darkens has just been finished the preparation of Chicken dung to black.
For the specific content that increases rhombus algae (for example Nitzschia palea), be added with the live body of a small amount of rhombus algae in the rich water agent of the present invention as the algae kind, when using, can effectively improve the ratio of rhombus algae in aquaculture water like this, even cultivate it and become dominant species in the water body planktonic organism, for the growth of pearl culturing clam provides sufficient good bait.The rhombus algae is bought from hydrobiont institute of Wuhan Chinese Academy of Sciences algae kind storehouse, and substratum is selected the D1 substratum.
In order to strengthen the effect of rich water preparation of the present invention, in the rich water preparation of preparation, also optionally be added into the viable bacteria of genus bacillus, milk-acid bacteria and bifidus bacillus.Wherein the substratum of genus bacillus is composed as follows: 1L distilled water+20g glucose+15g peptone+5g sodium-chlor+0.5g extractum carnis+20g agar; Milk-acid bacteria substratum (MRS solid medium) is as follows: peptone 10g, extractum carnis 10g, yeast extract paste 5g, K
2HPO
43H
2O 2g, sodium acetate 5g, glucose 20g, tween 80 1g, dibasic ammonium citrate 2gMgSO
47H
2O 0.58g, MnSO
44H
2O 0.25g, adding distil water 1000mL transfers PH6.2-6.4 at 121 ℃, and the 15min sterilization is for subsequent use.Medium of Bifidobacterium is selected the BS substratum, can buy the Bioisystech Co., Ltd from Qingdao Hai Bo.
After the substratum of genus bacillus, milk-acid bacteria, bifidus bacillus prepared, respectively bacterial classification is inoculated in the substratum according to 2% inoculum size, after the access, behind 35 ℃ of incubated overnight 24～48h, or measuring OD600 value is to stop cultivation at 0.5 o'clock.
The present invention is described further and verification experimental verification below in conjunction with embodiment.
Embodiment 1
The rich water preparation of the present embodiment is made by the raw material of following weight percent: fermentation poultry dung 91.5%, calcium superphosphate 3%, potassium primary phosphate 2.3%, calcium carbonate 0.5%, zinc sulfate 0.2%, manganous sulfate 0.5%, copper sulfate 0.5%, iron vitriol 0.5%, ammonium molybdate 0.2%, boric acid 0.3%.The said products is added the stirrer stirring and evenly mixing.Then rhombus algae liquid filtrate is joined in the rich water agent of preparation, air-dry.Making the weight percent of rhombus algae in the rich water agent is 0.5%, and can activity recovery when using.
Nitzschia palea is bought from hydrobiont institute of Wuhan Chinese Academy of Sciences algae kind storehouse, is numbered FACHB-211, and substratum is selected the D1 substratum.
Embodiment 2
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1, composed as follows after the preparation.
Fermentation poultry dung 88%, calcium superphosphate 4.5%, potassium primary phosphate 3.8%, calcium carbonate 0.5%, zinc sulfate 0.3%, manganous sulfate 0.7%, copper sulfate 0.6%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.2%, rhombus algae 0.5%.
Embodiment 3
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1.
Fermentation poultry dung 88%, calcium superphosphate 4.5%, potassium primary phosphate 3.8%, calcium carbonate 0.5%, zinc sulfate 0.3%, manganous sulfate 0.7%, copper sulfate 0.6%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.2%, rhombus algae 0.5%.
After preparing above-mentioned rich water preparation, the cultivation bacterium liquid adding of genus bacillus, milk-acid bacteria, bifidus bacillus is stirred, make the quantity of three kinds of bacterium in compound formulation be respectively 10
6～10
8Then CFU/kg makes the rich water agent of the present embodiment after the drying.Wherein genus bacillus can be selected available from Chinese agriculture microbial strains preservation center, is numbered the bacterial strain of 1511C0001ACCC02728; Bifidus bacillus is numbered 1511C0001ACCC11054 available from Chinese agriculture microbial strains preservation center; Milk-acid bacteria also available from Chinese agriculture microbial strains preservation center, is numbered 1511C0001ACCC10637.
Because the viable bacteria of having added probiotics in the rich water agent, before the rich water agent was used, bacterium also can again carry out biological degradation to fermentation poultry dung and process; Therefore, compare than the rich water agent of not adding bacterium, have extra beneficial effect in the time of use.
The using method of rich water compound formulation of the present invention:
With preparation water 1:10 dilution, the culture pond of splashing after mixing, 1 meter pond usage quantity of every mu of depth of water is 1-3Kg.Concrete usage quantity is decided on water colour, and the season of growth of the 5-10 month, every two weeks once.
The experimental study of rich water compound formulation:
Train water test in Huzhou, Zhejiang pearl freshwater mussel cultivation waters, 22 ℃ of water temperatures, 1 meter of the depth of water, the water surface are 5 mu, use the rich water compound formulation 10Kg of embodiment 1, around fetching water in each pond after the same day and 10 days, test reaches 1 part of each water sampling of central authorities, fully mixing is got 1 L mixed sample and is poured vial into, adds 5% formaldehyde solution and fixes, leave standstill 24-48h, concentrated rear at plant plankton counting frame microscopy, counting.Detect altogether 6 23 of planktonic microalgae in the sampling water quality on test same day and belong to 30 kinds.Wherein, Chlorophyta 8 belongs to 10 kinds, and Bacillariophyta 5 belongs to 5 kinds, and Pyrrophyta 5 belongs to 7 kinds, and Euglenophyta 3 belongs to 4 kinds, and Cyanophyta 1 belongs to a kind, and Chrysophyta 1 belongs to a kind.The planktonic microalgae cell quantity is between 8.33 * 10
7Ind/ L.Detect altogether 6 24 of planktonic microalgae in the water quality of sampling after 10 days and belong to 32 kinds.Wherein, Chlorophyta 8 belongs to 10 kinds, and Bacillariophyta 5 belongs to 6 kinds, and Pyrrophyta 5 belongs to 8 kinds, and Euglenophyta 3 belongs to 4 kinds, and Cyanophyta 1 belongs to 2 kinds, and Chrysophyta 2 belongs to 2 kinds.The planktonic microalgae cell quantity is between 4.51 * 10
8Ind/ L, wherein the rhombus algae 1.92 * 10
8Ind/ L.After using compound formulation, the algae in pearl culturing clam cultivation waters is many 2 kinds, and the planktonic microalgae cell quantity is 5.4 times before using, and the number of rhombus algae preponderates, and has rolled up the bait of pearl culturing clam, lays a solid foundation for bringing out high-quality pearl.
Adopt embodiment 2,3 rich water agent to carry out identical test, also can reach rich water effect of the present invention, the purpose that provides pearl culturing clam to grow required plant plankton and zooplankton is provided.And owing to having added probiotics among the embodiment 3, nacreous sickness rate is all lower than the preparation of embodiment 1 and 2 in cultivation.
Claims (3)
1. rich water compound formulation, it is characterized in that, include the material of following weight percent: Chicken dung 80～92%, calcium superphosphate 3～10%, potassium primary phosphate 1～5%, calcium carbonate 0.5～5%, zine ion 0.02～0.35%, mn ion 0.04～0.70%, cupric ion 0.1～0.6%, ferrous ion 0.1～0.7%, molybdenum ion 0.02～0.2%, boron ion 0.02～0.35%, rhombus algae 0.5～1%.
2. rich water compound formulation as claimed in claim 1 is characterized in that described rich water compound formulation also is added with genus bacillus, milk-acid bacteria and bifidus bacillus.
3. rich water compound formulation as claimed in claim 2 is characterized in that described genus bacillus, milk-acid bacteria and the bifidus bacillus quantity in compound formulation is respectively 10
6～10
8CFU/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210341995.9A CN102898208B (en) | 2012-09-16 | 2012-09-16 | Composite manure water preparation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210341995.9A CN102898208B (en) | 2012-09-16 | 2012-09-16 | Composite manure water preparation |
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| Publication Number | Publication Date |
|---|---|
| CN102898208A true CN102898208A (en) | 2013-01-30 |
| CN102898208B CN102898208B (en) | 2014-05-07 |
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ID=47570734
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|---|---|---|---|
| CN201210341995.9A Expired - Fee Related CN102898208B (en) | 2012-09-16 | 2012-09-16 | Composite manure water preparation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304142A (en) * | 2014-09-26 | 2015-01-28 | 浙江海洋学院 | Method for improving environment of penaeus penicillatus nursery pond |
| CN104370661A (en) * | 2014-10-22 | 2015-02-25 | 杨凌华辕生物产业集团有限公司 | Microecological preparation for grain production safety assurance and preparation method thereof |
| CN109438008A (en) * | 2018-11-23 | 2019-03-08 | 安徽好蕾曼珠宝有限公司 | A kind of Composite manure water preparation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304142A (en) * | 2014-09-26 | 2015-01-28 | 浙江海洋学院 | Method for improving environment of penaeus penicillatus nursery pond |
| CN104370661A (en) * | 2014-10-22 | 2015-02-25 | 杨凌华辕生物产业集团有限公司 | Microecological preparation for grain production safety assurance and preparation method thereof |
| CN109438008A (en) * | 2018-11-23 | 2019-03-08 | 安徽好蕾曼珠宝有限公司 | A kind of Composite manure water preparation |
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| Publication number | Publication date |
|---|---|
| CN102898208B (en) | 2014-05-07 |
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