CN102573453B - 生产活的四倍体牡蛎的方法 - Google Patents
生产活的四倍体牡蛎的方法 Download PDFInfo
- Publication number
- CN102573453B CN102573453B CN201080046057.5A CN201080046057A CN102573453B CN 102573453 B CN102573453 B CN 102573453B CN 201080046057 A CN201080046057 A CN 201080046057A CN 102573453 B CN102573453 B CN 102573453B
- Authority
- CN
- China
- Prior art keywords
- fertilized egg
- oyster
- polar body
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- dimethylaminopurine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 241000237502 Ostreidae Species 0.000 title claims abstract description 80
- 235000020636 oyster Nutrition 0.000 title claims abstract description 80
- 208000035199 Tetraploidy Diseases 0.000 title claims abstract description 26
- 238000004519 manufacturing process Methods 0.000 title description 9
- BVIAOQMSVZHOJM-UHFFFAOYSA-N N(6),N(6)-dimethyladenine Chemical compound CN(C)C1=NC=NC2=C1N=CN2 BVIAOQMSVZHOJM-UHFFFAOYSA-N 0.000 claims abstract description 128
- GBOGMAARMMDZGR-UHFFFAOYSA-N UNPD149280 Natural products N1C(=O)C23OC(=O)C=CC(O)CCCC(C)CC=CC3C(O)C(=C)C(C)C2C1CC1=CC=CC=C1 GBOGMAARMMDZGR-UHFFFAOYSA-N 0.000 claims abstract description 50
- GBOGMAARMMDZGR-TYHYBEHESA-N cytochalasin B Chemical compound C([C@H]1[C@@H]2[C@@H](C([C@@H](O)[C@@H]3/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C(=O)O[C@@]23C(=O)N1)=C)C)C1=CC=CC=C1 GBOGMAARMMDZGR-TYHYBEHESA-N 0.000 claims abstract description 50
- GBOGMAARMMDZGR-JREHFAHYSA-N cytochalasin B Natural products C[C@H]1CCC[C@@H](O)C=CC(=O)O[C@@]23[C@H](C=CC1)[C@H](O)C(=C)[C@@H](C)[C@@H]2[C@H](Cc4ccccc4)NC3=O GBOGMAARMMDZGR-JREHFAHYSA-N 0.000 claims abstract description 50
- 238000000034 method Methods 0.000 claims abstract description 30
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 55
- 210000004508 polar body Anatomy 0.000 claims description 44
- 229960001760 dimethyl sulfoxide Drugs 0.000 claims description 31
- 102000002322 Egg Proteins Human genes 0.000 claims description 22
- 108010000912 Egg Proteins Proteins 0.000 claims description 22
- 210000004681 ovum Anatomy 0.000 claims description 22
- 208000026487 Triploidy Diseases 0.000 claims description 21
- 210000004027 cell Anatomy 0.000 claims description 18
- 102000003743 Relaxin Human genes 0.000 claims description 15
- 108090000103 Relaxin Proteins 0.000 claims description 15
- 238000007599 discharging Methods 0.000 claims description 11
- 230000002265 prevention Effects 0.000 claims 3
- 235000013601 eggs Nutrition 0.000 description 57
- 230000004720 fertilization Effects 0.000 description 15
- 210000000349 chromosome Anatomy 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 239000013535 sea water Substances 0.000 description 6
- 210000000130 stem cell Anatomy 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 5
- GGWOXIDGSYROGX-UHFFFAOYSA-N Cytochalasin L Natural products N1C(=O)C23OC(=O)C=CC(=O)C(OC(C)=O)C(C)=CC(C)CC=CC3C3OC3(C)C(C)C2C1CC1=CC=CC=C1 GGWOXIDGSYROGX-UHFFFAOYSA-N 0.000 description 4
- GGWOXIDGSYROGX-XZRSCLCVSA-N [(1S,4E,7R,8Z,10S,12E,14S,15S,17R,18S,19S,20S)-20-benzyl-8,10,17,18-tetramethyl-3,6,22-trioxo-2,16-dioxa-21-azatetracyclo[12.8.0.01,19.015,17]docosa-4,8,12-trien-7-yl] acetate Chemical compound C[C@H]1[C@H]2[C@H](Cc3ccccc3)NC(=O)[C@]22OC(=O)\C=C\C(=O)[C@H](OC(C)=O)\C(C)=C/[C@@H](C)C\C=C\[C@H]2[C@@H]2O[C@]12C GGWOXIDGSYROGX-XZRSCLCVSA-N 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000237501 Crassostrea Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 210000003783 haploid cell Anatomy 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 241000548230 Crassostrea angulata Species 0.000 description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
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- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 1
- 241001522252 Crassostrea rivularis Species 0.000 description 1
- 241000237504 Crassostrea virginica Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000490567 Pinctada Species 0.000 description 1
- 241001464019 Pinctada margaritifera Species 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000001448 gametogenic effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006266 hibernation Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000009288 screen filtration Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
- A01K67/0334—Genetically modified Molluscs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/03—Animals modified by random mutagenesis, e.g. using ENU, chemicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/70—Invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Marine Sciences & Fisheries (AREA)
- Farming Of Fish And Shellfish (AREA)
- Meat, Egg Or Seafood Products (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2009-0075404 | 2009-08-14 | ||
| KR1020090075404A KR100989384B1 (ko) | 2009-08-14 | 2009-08-14 | 생육가능한 4배체 굴을 생산하는 방법 |
| KR1020100075514A KR20120013512A (ko) | 2010-08-05 | 2010-08-05 | 생육가능한 4배체 굴을 생산하는 방법 |
| KR10-2010-0075514 | 2010-08-05 | ||
| PCT/KR2010/005169 WO2011019166A2 (fr) | 2009-08-14 | 2010-08-06 | Procédé de production dhuîtres tétraploïdes viables |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102573453A CN102573453A (zh) | 2012-07-11 |
| CN102573453B true CN102573453B (zh) | 2014-05-07 |
Family
ID=43586617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201080046057.5A Active CN102573453B (zh) | 2009-08-14 | 2010-08-06 | 生产活的四倍体牡蛎的方法 |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JP5613240B2 (fr) |
| CN (1) | CN102573453B (fr) |
| CO (1) | CO6511223A2 (fr) |
| WO (1) | WO2011019166A2 (fr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9385308B2 (en) * | 2010-03-26 | 2016-07-05 | Qualcomm Incorporated | Perpendicular magnetic tunnel junction structure |
| CN104255586B (zh) * | 2014-08-15 | 2016-01-20 | 中国科学院南海海洋研究所 | 一种生产香港牡蛎全三倍体的时间点定量处理方法 |
| CN107494358B (zh) * | 2017-09-28 | 2019-11-01 | 中国科学院南海海洋研究所 | 一种香港牡蛎四倍体幼贝的制备方法 |
| CN109730008B (zh) * | 2019-03-11 | 2021-01-01 | 中国海洋大学 | 一种培育葡萄牙牡蛎四倍体的方法 |
| CN112535127A (zh) * | 2020-07-14 | 2021-03-23 | 厦门世倍海洋科技有限公司 | 葡萄牙牡蛎二倍体与香港巨牡蛎三倍体杂交生产异源四倍体的方法 |
| CN112535128A (zh) * | 2020-07-14 | 2021-03-23 | 厦门世倍海洋科技有限公司 | 葡萄牙牡蛎二倍体与太平洋牡蛎三倍体杂交生产异源四倍体的方法 |
| CN113678764B (zh) * | 2020-12-31 | 2023-08-04 | 青岛前沿海洋种业有限公司 | 一种生产四倍体牡蛎和种间杂交三倍体牡蛎的方法 |
| CN113951194B (zh) * | 2021-10-20 | 2022-12-13 | 青岛前沿海洋种业有限公司 | 一种福建牡蛎和熊本牡蛎种间杂交生产三倍体牡蛎的方法 |
| CN114208735B (zh) * | 2021-12-22 | 2023-02-28 | 中国科学院南海海洋研究所 | 一种利用回交育种技术培育香港牡蛎三倍体快速生长新品系的方法 |
| CN114600807A (zh) * | 2022-04-02 | 2022-06-10 | 厦门大学 | 一种皱纹盘鲍四倍体的诱导方法 |
| CN116406649B (zh) * | 2023-02-22 | 2024-03-19 | 中国科学院南海海洋研究所 | 一种提高牡蛎四倍体遗传多样性和构建四倍体稳定群系的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995019703A1 (fr) * | 1994-01-21 | 1995-07-27 | Rutgers, The State University Of New Jersey | Coquillage tetraploide |
| US20050149997A1 (en) * | 2003-11-04 | 2005-07-07 | Wolozin Benjamin L. | Sterilization of marine organisms by manipulation of DNA content |
| CN1545873A (zh) * | 2003-12-16 | 2004-11-17 | 中国海洋大学 | 利用6-二甲氨基嘌呤诱导牡蛎四倍体的方法 |
| FR2913982B1 (fr) * | 2007-03-23 | 2009-05-29 | Ifremer | Obtention de mollusques bivalves tetraploides a partir de geniteurs diploides |
-
2010
- 2010-08-06 CN CN201080046057.5A patent/CN102573453B/zh active Active
- 2010-08-06 WO PCT/KR2010/005169 patent/WO2011019166A2/fr active Application Filing
- 2010-08-06 JP JP2012524635A patent/JP5613240B2/ja active Active
-
2012
- 2012-03-09 CO CO12041581A patent/CO6511223A2/es unknown
Non-Patent Citations (5)
| Title |
|---|
| 岳文斌等.动物克隆.《动物繁殖新技术》.中国农业出版社,2003,(第一版),第281页. * |
| 李慷均.太平洋牡蛎四倍体育种研究.《中国优秀博硕士学位论文全文数据库(硕士) 农业科技辑》.2005,(第1期),第25-33页. * |
| 王昭萍等.牡蛎的养殖.《海水贝类养殖技术》.青岛海洋大学出版社,1998,(第一版),第21-22页. * |
| 电脉冲与化学激活联合处理对猪卵母细胞孤雌发育的影响;蔡元等;《中国农业大学学报》;20040630;第9卷(第3期);第21-24页 * |
| 蔡元等.电脉冲与化学激活联合处理对猪卵母细胞孤雌发育的影响.《中国农业大学学报》.2004,第9卷(第3期),第21-24页. |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011019166A2 (fr) | 2011-02-17 |
| CN102573453A (zh) | 2012-07-11 |
| CO6511223A2 (es) | 2012-08-31 |
| JP2013501518A (ja) | 2013-01-17 |
| JP5613240B2 (ja) | 2014-10-22 |
| WO2011019166A3 (fr) | 2011-07-14 |
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