CN102526217A - Formula of health-care medicine with functions of strengthening immunity and promoting sleep - Google Patents
Formula of health-care medicine with functions of strengthening immunity and promoting sleep Download PDFInfo
- Publication number
- CN102526217A CN102526217A CN201110309363XA CN201110309363A CN102526217A CN 102526217 A CN102526217 A CN 102526217A CN 201110309363X A CN201110309363X A CN 201110309363XA CN 201110309363 A CN201110309363 A CN 201110309363A CN 102526217 A CN102526217 A CN 102526217A
- Authority
- CN
- China
- Prior art keywords
- sleep
- melatonin
- health
- parts
- mice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a formula of a health-care medicine with functions of strengthening immunity and promoting sleep. The health-care medicine capsules are prepared from the raw materials in part by weight: 6 to 15 parts of broccoli extract, 6 to 9 parts of melatonin, 4.5 to 10 parts of lotus plumule extract, 1.5 to 5 parts of polygala tenuifolia, 4.5 to 6 parts of tuber fleeceflower stem, 4.5 to 12 parts of Chinese arborvitae seed and 3 to 10 parts of microcrystalline cellulose. The health-care medicine can improve the symptoms of insomnia and dreamful sleep of people, the health level of people, and the life quality of people who have low immunity, and does not have side effect or adverse reaction; and the product is safe, stable, and controllable in quality because a modern preparation technology and an advanced quality control method are adopted.
Description
Technical field
The present invention relates to a kind of health medicine, relate in particular to a kind of enhancing immunity, hypnotic functional health pharmaceutical formulation.
Background technology
Along with the quickening of development of times and rhythm of life, the continuous development of society, the fierceness day by day of competition, the life stress that people face is also increasing.The insomnia problem that professional population often has each colony that begun to extend.Insomnia is meant to fall asleep and maybe can't keeps sleep state, causes not having enough sleep.Be weighed into again and sleep and keep sleep disorder (DIMS); Chinese medicine is called again: be insomnia, insomnia, must not crouch, the unable to close the eyes; Be often can not obtain a kind of disease that ortho sleep is a characteristic; For a variety of causes causes that difficulty falling asleep, Depth of sleep or frequency are too short, early awakening and the length of one's sleep are not enough or of poor quality etc., the common reason that causes having a sleepless night mainly contains environment reason, individual factors, body reason, Nervous and Mental Factors, emotional factor etc.According to Traditional Chinese medical theory, the reason of insomnia is mainly the internal organs disorder, especially the YIN nourishing function of the warming YANG function of the heart and kidney can not coordination, deficiency of qi and blood, imbalance of YIN and YANG etc.Not have sleep just not have health be the medical research personnel according in recent years to the neodoxy that latest result proposed of sleep study.The quality of sleep quality relates to normally carrying out of each item functional activity when waking up.Relevant research shows, but sleep produces outside the new vitality human body except allaying tiredness, also with the ability that improves immunity, resist the disease substantial connection is arranged.Sleep disorder then has a strong impact on quality of life.Reach the change of life style with the quickening pace of modern life, the sickness rate of sleep disorder increases gradually, and has become a major issue of the current medical circle of puzzlement.
Sleep disorder increases along with the increase at age, and the sickness rate in the old people is especially high.World Health Organization (WHO) proposes, and the over-65s person of developed country is the old people, developing country more than 60 years old the person be the old people.China more than 60 years old aging population with annual 3.2% speed increment.Foreign study finds that the prevalence rate of sleep disorder is 30%-40% among the old people.International spiritual and neurological foundation investigation demonstration in 2002 has 45% to have sleep disorder in the population of China, its middle-aged and elderly people accounts for 56.7%.In various sleep disorder, insomnia is modal one type, and it is meant starting of sleep and keeps the generation obstacle, is sleep quality or up a kind of subjective experience the less than normal demand.Secular insomnia, but the serious harm old people is physically and mentally healthy, also is simultaneously accident and the dead high risk factor that takes place.So how safe and reasonable ground uses the medicine of sleeping peacefully, and improves the physically and mentally healthy level of old people, has become the research focus of life sciences.
China is engaged in the research and the production of natural drug; Advantageous condition is arranged; The rarity of Traditional Chinese Medicine, it has clinical experience in several thousand, from present pharmaceutical research trend; Searching new drug or discovery guide are combined to thing and it are carried out the important content that structural modification is a drug development from natural product, add growth in the living standard and also make people that the quality of life has been had higher pursuit.Thereby pay attention to health more, pay attention to sleeper effect.Under this background, this prescription utilization high-tech means and with free of contamination pure natural animals and plants be aided with the enhancing immunity that other functional components are raw material, the health product of hypnotic function arise at the historic moment, and develop rapidly.
Summary of the invention
For solving the problems of the technologies described above, the present invention provides a kind of enhancing immunity, hypnotic functional health pharmaceutical formulation; Can assist people build up resistance, the hypnotic quality is improved quality of life of insomniac.
Enhancing immunity of the present invention, hypnotic functional health pharmaceutical formulation are processed by the raw material that comprises following parts by weight: Caulis et Folium Brassicae capitatae 6-15, melatonin 6-9; Plumula Nelumbinis extract 4.5-10, Radix Polygalae 1.5-5, Caulis Polygoni Multiflori 4.5-6; Semen Platycladi 4.5-12, microcrystalline Cellulose 3-10.
The method for preparing of enhancing immunity of the present invention, hypnotic functional health medicine may further comprise the steps:
1) earlier Radix Polygalae 1.5-5 part, Caulis Polygoni Multiflori 4.5-6 part, Semen Platycladi 4.5-12 part are pulverized respectively, cross the 60-80 mesh sieve;
2) learnt from else's experience powder that step 1) obtains mixes with the raw material of following parts by weight: Caulis et Folium Brassicae capitatae 6-15, melatonin 6-9, Plumula Nelumbinis extract 4.5-10, microcrystalline Cellulose 3-10; Incorporation time 45 minutes;
3) through step 2) behind the mix homogeneously, carry out capsule then and fill, filling process is in strict accordance with inner quality standard control capsule filling weight, screens, polishing, inner packing again, carries out outer package then, at last by touchstone check warehouse-in.
Beneficial effect compared with prior art of the present invention is: health medicine capsule of the present invention can improve people's insomnia and dreamful sleep symptom; Improve people's health level; Improve immunocompromised person's quality of life, have no side effect and untoward reaction, and use modern preparation technique; Advanced method of quality control makes product accomplish safety, stable, quality controllable.
Description of drawings
Fig. 1 be in the described enhancing immunity of the embodiment of the invention, the hypnotic functional health pharmaceutical formulation melatonin to the circadian influence curve figure of mice sleep.
The specific embodiment
Below in conjunction with accompanying drawing and embodiment, specific embodiments of the invention describes in further detail.Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1
A kind of enhancing immunity, hypnotic functional health pharmaceutical formulation are processed by the raw material that comprises following weight (gram): Caulis et Folium Brassicae capitatae 15, melatonin 8, Plumula Nelumbinis extract 4.5, Radix Polygalae 1.5, Caulis Polygoni Multiflori 5, Semen Platycladi 4.5, microcrystalline Cellulose 6.
The method for preparing of enhancing immunity of the present invention, hypnotic functional health medicine may further comprise the steps:
1) earlier Radix Polygalae 1.5 grams, Caulis Polygoni Multiflori 5 grams, Semen Platycladi 4.5 grams are pulverized respectively, crossed the 60-80 mesh sieve;
2) learnt from else's experience powder that step 1) obtains mixes with the raw material of following weight (gram): Caulis et Folium Brassicae capitatae 15, melatonin 8, Plumula Nelumbinis extract 4.5, microcrystalline Cellulose 6; Incorporation time 45 minutes;
3) through step 2) behind the mix homogeneously, carry out capsule then and fill, filling process is in strict accordance with inner quality standard control capsule filling weight, screens, polishing, inner packing again, carries out outer package then, at last by touchstone check warehouse-in.
Embodiment 2
A kind of enhancing immunity, hypnotic functional health pharmaceutical formulation are processed by the raw material that comprises following weight (gram): Caulis et Folium Brassicae capitatae 12, melatonin 9, Plumula Nelumbinis extract 7, Radix Polygalae 4, Caulis Polygoni Multiflori 6, Semen Platycladi 8, microcrystalline Cellulose 8.
The method for preparing of described enhancing immunity, hypnotic functional health medicine is with embodiment 1.
Embodiment 3
A kind of enhancing immunity, hypnotic functional health pharmaceutical formulation are processed by the raw material that comprises following weight (gram): Caulis et Folium Brassicae capitatae 7, melatonin 9, Plumula Nelumbinis extract 10, Radix Polygalae 5, Caulis Polygoni Multiflori 6, Semen Platycladi 11, microcrystalline Cellulose 10.
The method for preparing of described enhancing immunity, hypnotic functional health medicine is with embodiment 1.
Enhancing immunity of the present invention, hypnotic functional health medicine can improve insomniac's symptom; Improve people's health level; Improve insomniac's quality of life, have no side effect and untoward reaction, and use modern preparation technique; Advanced method of quality control makes product accomplish safety, stable, quality controllable.
Each component in enhancing immunity of the present invention, the hypnotic functional health pharmaceutical formulation has following characteristics:
1) Caulis et Folium Brassicae capitatae extracts from Caulis et Folium Brassicae capitatae, and Caulis et Folium Brassicae capitatae has another name called broccoli, blue and white Lay, sprouting broccoli etc., belongs to the mutation that the Cruciferae Caulis et Folium Brassicae campestris belongs to brassica specie, is one, the biennial herb plant, originates in Italy.Existing experiment proof Caulis et Folium Brassicae capitatae contains abundant cancer-resisting substance and can lower the formation to the mouse breast tumor, stops the activity of carcinogen.Scientist finds that the anti-cancer material in the brassicaceous vegetables such as Caulis et Folium Brassicae capitatae is the enzymatic hydrolysate or the derivant of thioglycoside, wherein isothiocyanate class most importantly.Radix Raphani sulfur glycosides is a kind of of thioglycoside, extensively is present in the Caulis et Folium Brassicae capitatae, and Radix Raphani sulfur glycosides produces isothiocyanate through myrosinase enzymolysis or acid hydrolysis: sulforaphen.Sulforaphen is the strongest anti-cancer active matter of finding in the vegetable up to now, because of it finds to gain the name in Radix Raphani.The ocean son professor of Japan nutritionist good fortune man waits and passes through verification experimental verification: contained isothiocyanic acid ester derivant sulforaphen has treatment cancer and the effectiveness of killing leukocyte in the crucifers such as Brassica oleracea L.var.capitata and Caulis et Folium Brassicae capitatae, and this is to confirm that it has the effect of killing the leukaemia for the first time.Show that according to research Radix Raphani sulfur glycosides (3-methylsulfinyl acrylic thioglycoside) and 4-methylsulfinyl cyclobutenyl thioglycoside account for 78% of total glucosinolate content in the fresh Caulis et Folium Brassicae capitatae of freshly harvested.Under common content, Radix Raphani sulfur glycosides has important benefits with its catabolite to health, though Radix Raphani sulfur glycosides is not direct beneficial organism active substance, its hydrolyzate sulforaphen has the defencive function of enhances human body.
The relation of sulforaphen and immunocompetence mainly is: external existing research proof sulforaphen has the function that improves immunity.Experiment shows: gave BALB/c (4-6 age in week) mouse every day (every of 500ug/) feed sulforaphen in continuous 9 days; The total leukocyte counting was strengthened one times of amount in the 9th day; The quantity of medullary cell and esterase A positive cell has also increased by one times; The hemolysis plaque number obviously raises, and the synthetic antibody number obviously increases, and sulforaphen has also improved the activate the phagocytic capacity of peritoneal macrophage.Radix Raphani sulfur greatly reduces the higher leveled TNF-a that stimulates macrophage to produce by LPS (endotoxin) in addition.These results show that it is obvious that sulforaphen raising immunity and auxiliary obesity are improved the interior microenvironment effect of body.Show through zoopery in addition: sulforaphen is remarkable in the effect of anti-tumor aspect.Sulforaphen is after continuously giving mouse high dose (μ mol/kg every days 100) feed in 4-5 days, and the activity that II separates toxenzyme mutually in mouse liver, lung, mammary gland, pancreas, stomach, small intestinal and colon all has raising.Munday etc. have compared six kinds of abilities that ITCs induces QR (quinone reductase), GST Phase II such as (glutathione transferases) to separate toxenzyme in the mouse Different Organs.Test dose is μ mol/kg every days 40, and feeding is 5 days continuously.The result shows that all ITCs have shown the activity of in duodenum, stomach, bladder, inducing QR and GST, and wherein sulforaphen induces the activity of GST the strongest in duodenum and bladder, and the effect with bladder is the most remarkable again in all organs.Jackson etc. discover; F311 breast cancer cell to the BALB/c mouse when sulforaphen reaches 15nmol/L concentration has inhibitory action; Gave BALB/c mouse injection 15nmol/d in continuous 13 days, the sulforaphen of concentration, the F311 breast cancer cell of mouse reduces about 60%.
Above experimental data proof sulforaphen has significant raising immunity and antitumor efficacy; But because sulforaphen itself is very unstable; These article adopt its prerequisite Radix Raphani sulfur glycosides as main component, take Radix Raphani sulfur glycosides through the zoopery proof, become sulforaphen through enzymolysis in animal body; Can reach and directly take the identical effect of sulforaphen, and product is more stable.
These article any side effect and untoward reaction all do not occur through acute toxicology, nursing experiment in 30 days.These article adopt ethanol and water from the vegetable Caulis et Folium Brassicae capitatae, to extract in addition, do not have other pollution.The dosage that adopts is in usual Caulis et Folium Brassicae capitatae vegetable edible range.Show that this prescription is feasible, have good using value.
2) melatonin is a kind of endogenous material, works through the adjusting of hormonal system, and the metabolic pathway of oneself is arranged in vivo.Melatonin in the blood has 70%-75% after liver metabolism becomes 6-hydroxyl melatonin sulphate form, excretes through urine (80%) and excrement (20%); 5%-7% changes into 6-hydroxyl melatonin Portugal candy thuja acid form in addition, can not cause metabolite to accumulate in vivo; And its biological half-life is short, promptly reduces to normal person's physiological level at oral 7-8h, so its toxicity is minimum.Directly the melatonin of influence sleep is mainly regulated phase when sleeping under the normal condition, can play syngignoscism if obey melatonin under the wakefulness state outward.Melatonin is the excretory optical signal hormone of pinus, the circadian rhythm of regulating animal and season the rhythm and pace of moving things and body " Sleep-Wake " rhythm and pace of moving things aspect play an important role.Under the normal condition, human body melatonin levels in the daytime is lower.In the daytime using melatonin can make body temperature reduce 0.3-0.4 ℃.Giving bright light night stimulates the secretion that can suppress melatonin, makes fervescence, causes the nighttime sleep amount to reduce simultaneously.If obey the material of relevant melatonin class outward, the animal and human all had quick syngignoscism.
Because sunlight and melatonin have confidential relation.At the pinus position of brain, receive the stimulation of light after, just suppress the secretion of melatonin.Daytime, then the release of melatonin was suppressed as taking a solar bath well, can promote the release of melatonin at night, thereby obtain fragrant and sweet sleep.
Melatonin is with age growth, and secretion reduces, and therefore possibly be to cause one of reason that old people's sleep quality descends, sleep disorder increases.Suitably utilize melatonin, can improve the old people and often face that the time difference changes or day alternates with night staff's sleep quality.And nearest discovering, in fact the melatonin that always is used for Cure for insomnia has significant immunoregulation effect.
(1) melatonin is tested the influence of pentobarbital sodium sub-threshold lull dosage: mice is divided into 4 groups at random, and pentobarbital sodium sub-threshold lull dosage is 25mg/kg.Visible by table 1, po melatonin 120mg/kg can obviously increase the sleeping number of elements of mice, and pentobarbital sodium sub-threshold lull dosage is had synergism.
Table 1 melatonin is to the n=10 that influences of pentobarbital sodium sub-threshold lull dosage
Annotate: compare * P＜0.01 with matched group; * P＜0.05
(2) melatonin prolongs the pentobarbital sodium test length of one's sleep: mice is divided into 4 groups at random.Pentobarbital sodium dosage is 40mg/kg.Visible by table 2, but po melatonin 30 and 16mg/kg equal significant prolongation pentobarbital sodium length of one's sleep.
Table 2 melatonin is to the pentobarbital sodium influence of the length of one's sleep (
n=10 ∽ 12)
Annotate: compare * P＜0.01 with matched group; * P＜0.05
(3) melatonin is to the circadian influence of pentobarbital sodium induced hypnotic: visible by Fig. 1, the matched group pentobarbital sodium presents rhythmicity the length of one's sleep to be changed, and when peak value appeared at 12:00, valley was when 20:00.Can prolong the length of one's sleep of pentobarbital sodium behind the use melatonin, but the degree that prolongs depends on administration time, visible rhythm and pace of moving things position shifts to an earlier date mutually, and peak value appears at 08:00, and valley appears at 16:00.The effect that melatonin prolongs pentobarbital sodium length of one's sleep is different because of division of day and night round the clock.
(4) melatonin is tested the influence of mouse hypothalamus, cerebral cortex, brain stem GABA content: mice is divided into 3 groups at random.Visible by table 3, the melatonin 15mg/kg content of GABA in the hypothalamus that can obviously raise, but the rising of other brain district GABA content is not had obvious influence.
Table 3 melatonin is to the influence (
n=6 ∽ 8) of different cerebral district alpha-aminobutyric acid content
Annotate: compare * P＜0.05 with matched group
(5) melatonin is tested the influence of mouse hypothalamus, cerebral cortex, brain stem Glu content: mice is divided into 3 groups at random.Visible by table 4, melatonin 15 or 5mg/kg do not have obvious influence to each brain district GLU content of mice.
Table 4 melatonin is to the influence (
n=6 ∽ 8) of different cerebral district glutamic
The endogenous melatonin has the low synthetic and secretion rhythm and pace of moving things in unique high daytime at night; This physiological rhythm and pace of moving things is controlled by the photoperiod; Because melatonin secretion at midnight at most, also is called " dark hormone ", it plays an important role aspect the sleep awakening at the adjusting circadian rhythm.Some scholar thinks that it is to set up the time that sleep takes place that melatonin mainly acts on, and corrects the disorderly sleep awakening rhythm and pace of moving things.The method that this experiment adopt to prolong pentobarbital sodium length of one's sleep is observed the influence of melatonin to sleep, and each experiment all adopts the special messenger to observe.The result finds that single gives melatonin and can improve the sleeping number of elements of pentobarbital sodium sub-threshold lull dosage mice, prolongs pentobarbital sodium length of one's sleep, and visible single gives melatonin and has direct syngignoscism.
In sum, melatonin is for building up one's resistance to disease, and the hypnotic quality has goodish effect.
3) Plumula Nelumbinis extract extracts from Semen Nelumbinis, and Semen Nelumbinis are nymphaeaceae plant, have another name called Hibisci Mutabilis, is to have the perennial water plant of expanding rhizome.Semen Nelumbinis are felt concerned about the dry spire and the radicle of the mature seed of lotus.Its nature and flavor bitter cold of Compendium of Material Medica record, GUIXIN lung kidney 3 warps, tool clears away heart-fire and purges heat, the effect of controlling nocturnal emission with astringent drugs hemostasis.The traditional Chinese medical science is used for the treatment of cards such as thermal man's pericardium, disarmony between the heart and kidney, insomnia seminal emission, heat in blood haematemesis.Modern study shows: Plumula Nelumbinis contains chemical constituents such as alkaloid, flavone, sitosterol, volatile oil and trace element; Have blood pressure lowering, arrhythmia, antiplatelet aggregation, anti peroxidation of lipid and remove free radical, pharmacological action such as anticancer.What in its numerous chemical constituents, have obvious biological activity and physiological function is flavone and these two big types of materials of alkaloid.Wherein flavones ingredient mainly is rutin and Quercetin.The alkaloids composition mainly is a nuciferine, nuciferine, 0 one demethyl lotus alkali.The Plumula Nelumbinis beginning is stated from " feeding habits book on Chinese herbal medicine ", has another name called the lotus heart of a lotus seed.Be the blue or green tender plumule in the Semen Nelumbinis.Its nature and flavor bitter cold is gone into the heart, lung, kidney three warps, has the effect of clearing away heart-fire for tranquillization, restoring normal coordination between the heart and kidney, unsmoothing the sperm and stopping bleeding.All include in the ancient books and records such as Compendium of Material Medica, " Japan hanako materia medica ", " the big bright will that unifies ", " book on Chinese herbal medicine is new again ", " medical center bun will ".Form with Semen Nelumbinis, Rhizoma Coptidis, the Radix Rehmanniae, Semen Ziziphi Spinosae, Poria cum Radix Pini, Radix Polygalae cooperation.Serve on 1 month, nocturnal emission just can reduce, and this side comes from " Dong-eui-bo-gam ".Its clearing away heart-fire of clinical report, suppressing the hyperactive liver fire, rush down stomach-fire, fall lung-fire, can treat the vexed insomnia of old people, adolescence's hyperactivity of heart-fire etc.
4) Radix Polygalae is a conventional Chinese medicine, and the beginning is stated from Shennong's Herbal, and quilt is classified as top grade, is the key medicine of supporting life.Have intelligence promoting and the mind tranquilizing, eliminating phlegm for diminishing swelling effect, be usually used in treating diseases such as insomnia and dreamful sleep, forgetful palpitation with fear, staring spells, expectoration that disarmony between the heart and kidney causes are not well, sore swollen toxin, breast swell and pain clinically.Say in " book on Chinese herbal medicine justice " " Radix Polygalae, the wholwe-hearted kidney of merit, so but the relieving palpitation spasmolytic, the peace that exorcises evil spirits dream, Yang strengthening and life essence tonifying, strong will power-assisted.
(1) Radix Polygalae is tested the influence of mice autonomic activities: compare with blank control group, the autonomic activities number of times obviously reduces after 1, No. 2 mice administrations of Radix Polygalae group, and the autonomic activities number of times also obviously reduces after 3, No. 4 mice administrations of low dosage Radix Polygalae group.Under different condition, Radix Polygalae all can reduce mice autonomic activities number of times and obviously have sedation.See table 5.
Table 5 Radix Polygalae is to the influence (
n=10) of mice autonomic activities
Annotate: compare * P＜0.05, * * P＜0.01 with matched group
(2) influence of the sleeping number of elements of mice is tested: compare with blank control group, the sleeping number of elements of No. 3 high dose group of Radix Polygalae and No. 6 low dose group mices of Radix Polygalae obviously increases (P＜0.010), and the prompting Radix Polygalae has no barbital increases the sleeping number of elements of mice by sodium effect for 3, No. 6.Table 6.
Table 6 various dose is to the influence of the sleeping number of elements of mice
Annotate: compare * * P＜0.01 with matched group
5) Caulis Polygoni Multiflori different name Caulis Polygoni Multiflori is trailing plants section plant Radix Polygoni Multiflori ratan.Has tranquilizing by nourishing the heart, the effect of collateral dredging wind dispelling.This experimentation is intended through to the tracing and analyzing of rat brain electrograph (EEG), and confirms the influence of Caulis Polygoni Multiflori to the rat sleep cycle, for its tranquilizing by nourishing the heart effect provides experimental basis.
When (2) Caulis Polygoni Multiflori is slept to normal rat to influence test: can know from table 7; Caulis Polygoni Multiflori can obviously reduce the awakening time (W) (P＜0.01) of rat; Prolong TST (P＜0.01); When sleep, go up Caulis Polygoni Multiflori mutually and mainly show as prolongation SWS2 (P＜0.05) and SWS1 (P＜0.01), can increase SWS.
The influence of phase when table 7 Caulis Polygoni Multiflori is slept to normal rat
Annotate: compare * P＜0.01 with matched group
Result of the test shows: Caulis Polygoni Multiflori decocting fluid power prolongs free movable rat total length of one's sleep (P＜0.01), when sleep, go up mutually mainly to show as to prolong SWS1, and SWS2, to not significantly influence of REMS.Show that in view of the above Caulis Polygoni Multiflori decocting fluid power enough promotes body development, accelerate tired recovery, prolong sleep.
(3) the promotion test of Caulis Polygoni Multiflori normal rat sleep
Table 8 barbital sodium sub-threshold dose hypnosis experimental result
Annotate: compare * P＜0.05, * * P＜0.01 with matched group
Visible by table 8: low, high dose Caulis Polygoni Multiflori glycoside with low in dosage Caulis Polygoni Multiflori flavone compare the sleeping rate of mice with matched group and be significant difference (comparing P＜0.05) and show that it has facilitation to the sleeping rate that increases injection sub-threshold dose barbital sodium mice with the normal control group; Rate that the Caulis Polygoni Multiflori glycoside is low, the animal of high dose falls asleep is apparently higher than blank water; Yet but not having dependency relation between the dose groups, is that the difference of live body causes in the zoopery, simultaneously the animal of the basic, normal, high dosage of Caulis Polygoni Multiflori anthraquinone; A sleeping animal number average is higher than blank water; And in rising trend with the sleeping number of animals of the rising of dosage, each dose groups and blank water difference not significantly (inspection through card side) can significantly increase the sleeping rate of animal if suitably improve dosage.
Visible by following table 9, high dose Caulis Polygoni Multiflori glycoside is compared mouse sleep time and is significant difference (comparing P＜0.05 with the normal control group) with matched group, shows that it is to having facilitation the length of one's sleep that prolongs injection pentobarbital sodium mice.
Table 9 prolongs the pentobarbital sodium experimental result length of one's sleep
The Caulis Polygoni Multiflori glycoside demonstrates the effect of certain improvement sleep in this research; In barbital sodium sub-threshold dose hypnosis experiment, compare with matched group; The sleeping rate of mice is significant difference (comparing P＜0.05 with the normal control group) in barbital sodium sleep experiment incubation period, and Caulis Polygoni Multiflori glycoside high dose is compared mouse sleep time and is significant difference (comparing P＜0.05 with the normal control group) and explains that it has facilitation for prolonging mouse sleep time with matched group.
6) the Semen Platycladi nature and flavor are sweet, flat, GUIXIN, liver, spleen channel.The function the heart nourishing and intellengence improving, calm the nerves, moisturize, loosening bowel to relieve constipation, cure mainly the neurasthenia, symptoms such as insomnia, fidgets due to deficiency.
(1) direct sleep experiments result: by national standard method three kinds of active component in the Semen Platycladi are carried out direct sleep experiments, the result sees table 10.
Table 10 number of animals (n=12) of directly sleeping in the sleep experiments
Visible by table 10; The number of animals of directly sleeping in 11 treated animals in the sleep experiments is 0; Be in the Semen Platycladi each composition extremely matched group mice is not all had direct syngignoscism; Meet and " improve the requirement of sleep health food in health food check and the assessment technique enforcement of regulations handbook, can carry out other three experiments.
(2) barbital sodium sub-threshold dose hypnosis experimental result: by national standard method three kinds of active component in the Semen Platycladi are carried out barbital sodium sub-threshold dose hypnosis experiment, study its influence to the mice sleep rate, the result sees table 11.
Table 11 Semen Platycladi composition is to the influence of mice sleep rate
Annotate: with blank control group comparison separately, ※ P＜0.05.
Visible by table 11; High dose Semen Platycladi fatty oil is compared with matched group with high dose Semen Platycladi glycoside; The sleeping rate of mice is significant difference (comparing P＜0.05 with the normal control group), shows that it all has facilitation to the sleeping rate that increases injection sub-threshold dose barbital sodium mice.The sleeping rate of the animal of Semen Platycladi fatty oil high dose is apparently higher than blank group (P＜0.05), though and the sleeping rate of the animal of basic, normal, high dosage is slightly higher than matched group, difference is not obvious.The sleeping animal index of the animal of the basic, normal, high dosage of Semen Platycladi volatile oil is slightly in rising trend with the rising of dosage; But each dose groups is than blank water group; Difference is not significantly (through X 2 test) all, and prompting suitably improves dosage, and the possibility of the sleeping rate of remarkable increase animal is arranged.The basic, normal, high three dose groups sleep of Semen Platycladi glycoside animal number average is higher than the blank group, and wherein high dose Semen Platycladi glycoside group is than blank control group tool significant difference (P＜0.05).In addition, along with increasing of Semen Platycladi glycoside dosage, the sleep number of animals is slightly on the rise.
(3) to the influence of the sleeping number of elements of mice: through to the test of mice sedative-hypnotic effect, observed mice pentobarbital sodium sub-threshold dose hypnosis time and sleep delay time respectively, the result is as shown in table 1.With the blank group relatively, the Semen Platycladi saponin high dose group mice number of elements showed increased of falling asleep, middle low dose group is not obvious.Three dose groups of oriental arborvitae seed oil have increased the mice sleep number of elements to some extent, and wherein the low dose group effect is obvious, and high, middle dose groups effect is suitable.
Table 12 Semen Platycladi saponin and oriental arborvitae seed oil are to the down influence of sleep mice number of elements of mice threshold (X ± S)
(4) barbital sodium sleep experimental result incubation period: by national standard method three kinds of active component in the Semen Platycladi are carried out barbital sodium sleep experiment incubation period, study its influence to the mice time for falling asleep, the result sees table 13.It is thus clear that each dose groups of Semen Platycladi fatty oil is compared with matched group tween group, mice sleep all is significant difference (P＜0.05) incubation period, shows that the Semen Platycladi fatty oil promotes the sleeping function well of mice.Simultaneously; Low, middle dosage Semen Platycladi glycoside also can significantly shorten mice sleep incubation period (P＜0.05); But high dose Semen Platycladi glycoside is than the sleeping slightly difficulty (though there was no significant difference) of blank group mice; Show that low, the middle dosage of this composition shortens mice sleep effect incubation period significantly, dosage is too high, and then possibly to suppress mice sleeping.In addition, low dosage Semen Platycladi volatile oil is compared with matched group, also can significantly shorten mouse sleep time (P＜0.05).
Table 13 Semen Platycladi composition is to the preclinical influence of mice sleep (X ± S)
Annotate: compare ※ P＜0.05 with blank control group.
The sleep of each dose groups of Semen Platycladi fatty oil and blank tween group compares incubation period, all is lower than blank tween and has statistical significance (comparing P＜0.05 with matched group); Compare with blank tween group, low dosage Semen Platycladi volatile oil can significantly shorten mice sleep incubation period (P＜0.05), and middle and high dose groups is compared difference with matched group not obvious; Low, the middle dosage mice sleep of Semen Platycladi glycoside incubation period then with blank water comparing difference significantly (P＜0.05), this composition high dose group mice sleep incubation period is then a little more than blank water group.
(5) prolong the pentobarbital sodium experimental result length of one's sleep: by national standard method three kinds of active component in the Semen Platycladi are prolonged the pentobarbital sodium experiment length of one's sleep, study its influence to mouse sleep time, the result sees table 14.
Each composition of table 14 Semen Platycladi is to the influence of mouse sleep time (X ± S)
Annotate: compare ※ P＜0.05 with blank control group.
Visible by table 13, middle and high dosage Semen Platycladi volatile oil contrast tween group is compared, and mouse sleep time is remarkable difference and (compares with matched group; P＜0.05); Show it to having facilitation the length of one's sleep that prolongs injection pentobarbital sodium mice, though and low dosage Semen Platycladi volatilization line of oils is slightly longer than matched group mouse sleep time, difference is not remarkable; Prompting possibly cause it and prolong the mouse sleep time action effect inferior to middle and high dose groups because dosage is not enough.The middle and high dose groups mouse sleep time of Semen Platycladi volatile oil is all apparently higher than blank water group, though low dosage has prolongation trend, but difference is not remarkable, shows that Semen Platycladi volatile oil tool prolongs the mouse sleep time effect, and effect degree and dosage positive correlation.
In sum, high dose Semen Platycladi fatty oil shortens the equal tool facilitations in aspect such as animal time for falling asleep to improving laboratory animal sleep rate.
7) microcrystalline Cellulose; Consider that Caulis et Folium Brassicae capitatae, melatonin, Plumula Nelumbinis, Radix Polygalae, Caulis Polygoni Multiflori, Semen Platycladi contain sugar and the higher characteristics of vitamin; And the operability of the course of processing we selected the food additive microcrystalline Cellulose for use, show in " one one kinds of multi-functional macromolecular materials of microcrystalline Cellulose " literary composition of on " Guangzhou chemical industry " 1993 the 21st the 3rd phases of volume, delivering according to the Zhu Yu of Guangzhou Inst of Chemistry, Chinese Academy of Sciences qin, Tang Liegui, Pan Songhan: microcrystalline Cellulose can be used as antiplastering aid.The microcrystalline Cellulose addition meets GB2760 " food additive use sanitary standard " for 15% of prescription, and microcrystalline Cellulose toxicity property ADI does not do particular provisions (FAO/WHO, 1994), makes its whole prescription have more reasonability and science like this.
Several kinds of raw materials are assisted mutually in whole prescription and are deposited mutually more than this specific embodiment, bring out the best in each other, and make full side present effect preferably, and product has tangible enhancing immunity, hypnotic function through these article of function test proof.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and modification, these improve and modification also should be regarded as protection scope of the present invention.
Claims (1)
1. an enhancing immunity, hypnotic functional health pharmaceutical formulation is characterized in that, are processed by the raw material that comprises following parts by weight: Caulis et Folium Brassicae capitatae 6-15; Melatonin 6-9; Plumula Nelumbinis extract 4.5-10, Radix Polygalae 1.5-5, Caulis Polygoni Multiflori 4.5-6; Semen Platycladi 4.5-12, microcrystalline Cellulose 3-10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110309363.XA CN102526217B (en) | 2011-10-13 | 2011-10-13 | Formula of health-care medicine with functions of strengthening immunity and promoting sleep |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110309363.XA CN102526217B (en) | 2011-10-13 | 2011-10-13 | Formula of health-care medicine with functions of strengthening immunity and promoting sleep |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102526217A true CN102526217A (en) | 2012-07-04 |
| CN102526217B CN102526217B (en) | 2014-03-05 |
Family
ID=46335038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110309363.XA Expired - Fee Related CN102526217B (en) | 2011-10-13 | 2011-10-13 | Formula of health-care medicine with functions of strengthening immunity and promoting sleep |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102526217B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187597A (en) * | 2013-09-24 | 2014-12-10 | 刘凤琪 | "Quanlian" nerve-calming healthcare capsule |
| CN105168696A (en) * | 2015-10-19 | 2015-12-23 | 广西中医药大学 | Zhuang medicine composition for treating insomnia and preparation method thereof |
| CN106389934A (en) * | 2016-12-07 | 2017-02-15 | 广东伊茗药业有限公司 | Oral dissolution tablets capable of accelerating sleep |
| CN107375722A (en) * | 2017-08-17 | 2017-11-24 | 海宁市盈向家居设计有限公司 | A kind of sachet of home therapy insomnia |
| CN110376092A (en) * | 2019-08-28 | 2019-10-25 | 西南医科大学 | A kind of compound haw thorn Content of Chlorogenic Acid and content of hesperidin measurement and integrated evaluating method |
| CN111973721A (en) * | 2020-07-29 | 2020-11-24 | 成都润馨堂药业有限公司 | Pharmaceutical composition for improving sleep and preparation method and application thereof |
| JP2021048806A (en) * | 2019-09-26 | 2021-04-01 | カゴメ株式会社 | Melatonin production promoter and sleep quality improver |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1408397A (en) * | 2001-09-26 | 2003-04-09 | 北京本元青阳生物科技发展有限公司 | Composition containing melatonin and galangal fruit traditional Chinese medicine |
| CN1660400A (en) * | 2003-12-31 | 2005-08-31 | 赵恒� | Medication for improving sleep |
| US20070207134A1 (en) * | 2004-03-31 | 2007-09-06 | Calpis Co., Ltd | Functional food for ameliorating engogenous melatonin secretion rhythm and functional food for ameliorating circadian rhythm |
| WO2009111486A2 (en) * | 2008-03-04 | 2009-09-11 | The Regents Of The University Of California | Reversing the immune decline of aging by a nutraceutical antioxidant in mice |
| CN101664428A (en) * | 2009-03-20 | 2010-03-10 | 宁波海逸生物科技有限公司 | Broccoli polyphenol capsules |
| CN101695522A (en) * | 2009-10-30 | 2010-04-21 | 北京师范大学 | Chinese medicinal composition for treating insomnia |
-
2011
- 2011-10-13 CN CN201110309363.XA patent/CN102526217B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1408397A (en) * | 2001-09-26 | 2003-04-09 | 北京本元青阳生物科技发展有限公司 | Composition containing melatonin and galangal fruit traditional Chinese medicine |
| CN1660400A (en) * | 2003-12-31 | 2005-08-31 | 赵恒� | Medication for improving sleep |
| US20070207134A1 (en) * | 2004-03-31 | 2007-09-06 | Calpis Co., Ltd | Functional food for ameliorating engogenous melatonin secretion rhythm and functional food for ameliorating circadian rhythm |
| WO2009111486A2 (en) * | 2008-03-04 | 2009-09-11 | The Regents Of The University Of California | Reversing the immune decline of aging by a nutraceutical antioxidant in mice |
| CN101664428A (en) * | 2009-03-20 | 2010-03-10 | 宁波海逸生物科技有限公司 | Broccoli polyphenol capsules |
| CN101695522A (en) * | 2009-10-30 | 2010-04-21 | 北京师范大学 | Chinese medicinal composition for treating insomnia |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187597A (en) * | 2013-09-24 | 2014-12-10 | 刘凤琪 | "Quanlian" nerve-calming healthcare capsule |
| CN105168696A (en) * | 2015-10-19 | 2015-12-23 | 广西中医药大学 | Zhuang medicine composition for treating insomnia and preparation method thereof |
| CN105168696B (en) * | 2015-10-19 | 2019-10-18 | 广西中医药大学 | It is a kind of to treat the strong drug composition and preparation method thereof being insomnia |
| CN106389934A (en) * | 2016-12-07 | 2017-02-15 | 广东伊茗药业有限公司 | Oral dissolution tablets capable of accelerating sleep |
| CN107375722A (en) * | 2017-08-17 | 2017-11-24 | 海宁市盈向家居设计有限公司 | A kind of sachet of home therapy insomnia |
| CN110376092A (en) * | 2019-08-28 | 2019-10-25 | 西南医科大学 | A kind of compound haw thorn Content of Chlorogenic Acid and content of hesperidin measurement and integrated evaluating method |
| JP2021048806A (en) * | 2019-09-26 | 2021-04-01 | カゴメ株式会社 | Melatonin production promoter and sleep quality improver |
| JP7499013B2 (en) | 2019-09-26 | 2024-06-13 | カゴメ株式会社 | Melatonin production promoter and sleep quality improver |
| CN111973721A (en) * | 2020-07-29 | 2020-11-24 | 成都润馨堂药业有限公司 | Pharmaceutical composition for improving sleep and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102526217B (en) | 2014-03-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101695376B (en) | Health-care food for preventing diabetes | |
| CN102526217B (en) | Formula of health-care medicine with functions of strengthening immunity and promoting sleep | |
| CN102784295B (en) | Health-care preparation formula for improving female climacteric syndrome and preparation method of same | |
| CN103041264B (en) | Kudzuvine root brain-boosting and intelligence-boosting oral solution with function of preventing vascular dementia and preparation method thereof | |
| CN103719493B (en) | Siberian solomonseal rhizome health-care tea of a kind of brain-nourishing intelligence-benefiting and preparation method thereof | |
| CN102335330A (en) | Traditional Chinese medicine composition for treating insomnia | |
| CN103461585B (en) | Qi tonifying and health preserving herbal tea | |
| CN105434857A (en) | Health-care finger citron oral solution for soothing liver, removing blood stasis and fading freckles as well as preparation method of oral solution | |
| CN103181434A (en) | Comfortable health-care tea and preparation method thereof | |
| CN103749819B (en) | Fructus Corni health protection tea of a kind of hypoglycemic and preparation method thereof | |
| CN102526456A (en) | Health-care medicine formula capable of enhancing immunity and promoting sleep function | |
| CN104522213A (en) | Tea suitable for three-high crowds and making method of tea suitable for three-high crowds | |
| CN104206595A (en) | Blood lipid and blood glucose reducing Chinese wolfberry root-bark health care tea and preparation method thereof | |
| CN104544084B (en) | A kind of health food for diabetics | |
| CN103520302B (en) | Sea-buckthorn leaf anti-fatigue capsule and preparation method thereof | |
| CN102726589A (en) | Chewing gum specially for diabetic patients and preparation method thereof | |
| CN102579816B (en) | Brain-invigorating tablets and preparation method thereof | |
| CN104432052A (en) | Health capsules capable of resisting fatigue and preventing aging and preparation method thereof | |
| CN100382832C (en) | Health foods in use for improving symptom in climacteric period of female and fabricating method | |
| CN103525640B (en) | Energy-keeping yang-tonifying wine | |
| CN101020021A (en) | Antilipemic health product | |
| CN104474103A (en) | Natural plant hypoglycemic agent and preparation method thereof | |
| CN108524861A (en) | A kind of traditional Chinese medicinal ointment and preparation method thereof for treating insomnia | |
| CN101020041A (en) | Antilipemic health product | |
| CN102250736A (en) | Health-care medicated wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140305 Termination date: 20141013 |
|
| EXPY | Termination of patent right or utility model |