CN102492747B - 一种酶法制备分离l-天门冬氨酸及其副产品硫酸铵的方法 - Google Patents
一种酶法制备分离l-天门冬氨酸及其副产品硫酸铵的方法 Download PDFInfo
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- CN102492747B CN102492747B CN201110377559.2A CN201110377559A CN102492747B CN 102492747 B CN102492747 B CN 102492747B CN 201110377559 A CN201110377559 A CN 201110377559A CN 102492747 B CN102492747 B CN 102492747B
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- Prior art keywords
- ammonium sulfate
- aspartic acid
- byproduct
- aspartate
- mother liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 title claims abstract description 41
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 229910052921 ammonium sulfate Inorganic materials 0.000 title claims abstract description 33
- 235000011130 ammonium sulphate Nutrition 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 31
- 239000006227 byproduct Substances 0.000 title claims abstract description 20
- 238000005406 washing Methods 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims abstract description 15
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000019297 ammonium fumarate Nutrition 0.000 claims abstract description 7
- CKKXWJDFFQPBQL-SEPHDYHBSA-N azane;(e)-but-2-enedioic acid Chemical compound N.N.OC(=O)\C=C\C(O)=O CKKXWJDFFQPBQL-SEPHDYHBSA-N 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims abstract description 7
- 239000001729 Ammonium fumarate Substances 0.000 claims abstract description 6
- 238000001556 precipitation Methods 0.000 claims abstract description 5
- 235000003704 aspartic acid Nutrition 0.000 claims description 38
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 34
- 239000012452 mother liquor Substances 0.000 claims description 28
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 12
- 238000002425 crystallisation Methods 0.000 claims description 9
- 230000008025 crystallization Effects 0.000 claims description 9
- 230000005611 electricity Effects 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 4
- 150000001510 aspartic acids Chemical class 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 230000008719 thickening Effects 0.000 claims description 4
- BFXUWDKAQDARCA-DKWTVANSSA-N (2s)-2-aminobutanedioic acid;azane Chemical compound [NH4+].OC(=O)[C@@H](N)CC([O-])=O BFXUWDKAQDARCA-DKWTVANSSA-N 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 abstract description 11
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 6
- 229910021529 ammonia Inorganic materials 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 230000002378 acidificating effect Effects 0.000 abstract description 2
- 239000002360 explosive Substances 0.000 abstract description 2
- 239000010413 mother solution Substances 0.000 abstract 4
- 239000013078 crystal Substances 0.000 abstract 2
- 229940009098 aspartate Drugs 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 238000004042 decolorization Methods 0.000 abstract 1
- YEOCBTKAGVNPMO-JIZZDEOASA-N diazanium;(2s)-2-aminobutanedioate Chemical compound [NH4+].[NH4+].[O-]C(=O)[C@@H](N)CC([O-])=O YEOCBTKAGVNPMO-JIZZDEOASA-N 0.000 abstract 1
- 229960005261 aspartic acid Drugs 0.000 description 28
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 12
- 239000001530 fumaric acid Substances 0.000 description 6
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 6
- 239000007787 solid Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000000967 suction filtration Methods 0.000 description 4
- 241000432824 Asparagus densiflorus Species 0.000 description 3
- HIVLDXAAFGCOFU-UHFFFAOYSA-N ammonium hydrosulfide Chemical compound [NH4+].[SH-] HIVLDXAAFGCOFU-UHFFFAOYSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 1
- CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960004756 ethanol Drugs 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Abstract
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CN201110377559.2A CN102492747B (zh) | 2011-11-23 | 2011-11-23 | 一种酶法制备分离l-天门冬氨酸及其副产品硫酸铵的方法 |
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CN201110377559.2A CN102492747B (zh) | 2011-11-23 | 2011-11-23 | 一种酶法制备分离l-天门冬氨酸及其副产品硫酸铵的方法 |
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CN102492747A CN102492747A (zh) | 2012-06-13 |
CN102492747B true CN102492747B (zh) | 2014-04-30 |
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CN107475320A (zh) * | 2017-08-23 | 2017-12-15 | 安徽雪郎生物科技股份有限公司 | L‑天门冬氨酸的绿色生产方法 |
CN110818152A (zh) * | 2019-11-13 | 2020-02-21 | 宜兴市前成生物有限公司 | 一种富马酸生产废水综合利用方法 |
CN112391424A (zh) * | 2020-11-12 | 2021-02-23 | 山东省食品发酵工业研究设计院 | 一种l-天冬氨酸的清洁提取工艺 |
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CN101586131B (zh) * | 2009-06-12 | 2011-12-28 | 南京工业大学 | 一种制备l-天冬氨酸的方法 |
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Denomination of invention: Method for enzymatically preparing and separating L-aspartate and ammonium sulfate as byproduct thereof Effective date of registration: 20150520 Granted publication date: 20140430 Pledgee: Jiangsu Yixing rural commercial bank Limited by Share Ltd new sub branch Pledgor: Yixing Qiancheng Biological Co., Ltd. Registration number: 2015990000390 |
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Date of cancellation: 20160120 Granted publication date: 20140430 Pledgee: Jiangsu Yixing rural commercial bank Limited by Share Ltd new sub branch Pledgor: Yixing Qiancheng Biological Co., Ltd. Registration number: 2015990000390 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for enzymatically preparing and separating L-aspartate and ammonium sulfate as byproduct thereof Effective date of registration: 20160120 Granted publication date: 20140430 Pledgee: Jiangsu Yixing rural commercial bank Limited by Share Ltd new sub branch Pledgor: Yixing Qiancheng Biological Co., Ltd. Registration number: 2016990000063 |
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PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20180516 Granted publication date: 20140430 Pledgee: Jiangsu Yixing rural commercial bank Limited by Share Ltd new sub branch Pledgor: Yixing Qiancheng Biological Co., Ltd. Registration number: 2016990000063 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for enzymatically preparing and separating L-aspartate and ammonium sulfate as byproduct thereof Effective date of registration: 20180516 Granted publication date: 20140430 Pledgee: Jiangsu Yixing rural commercial bank Limited by Share Ltd new sub branch Pledgor: Yixing Qiancheng Biological Co., Ltd. Registration number: 2018990000365 |
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