CN102488251A - Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin - Google Patents
Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin Download PDFInfo
- Publication number
- CN102488251A CN102488251A CN2011104112801A CN201110411280A CN102488251A CN 102488251 A CN102488251 A CN 102488251A CN 2011104112801 A CN2011104112801 A CN 2011104112801A CN 201110411280 A CN201110411280 A CN 201110411280A CN 102488251 A CN102488251 A CN 102488251A
- Authority
- CN
- China
- Prior art keywords
- sea cucumber
- glue
- sea
- cucumber
- pretreated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention provides a sea cucumber gelatin, which is prepared by the following steps of: 1, preprocessing sea cucumbers; 2, extracting a gelatin from the preprocessed sea cucumbers to obtain a sea cucumber gelatin liquor; 3, removing heavy metals from the sea cucumber gelatin liquor obtained in the step 2; and 4, concentrating the sea cucumber gelatin liquor from which the heavy metals are removed, and molding. The invention provides a sea cucumber total nutrient concentrated edible sea cucumber gelatin and a preparation method thereof. Compared with the conventional sea cucumber dried products, capsules, oral solutions and other sea cucumber products, the sea cucumber gelatin maintains a large number of nutrients substances and bioactive components in the sea cucumbers, overcomes the defect that the conventional sea cucumber products cannot fully utilize sea cucumber proteins, has higher nutritional value and can comprehensively and quickly supplement nutrients for a human body.
Description
Technical field
The present invention relates to a kind of cucumber product, relate in particular to a kind of beche-de-mer glue.
Background technology
Sea cucumber is one of the world's eight big treasures; It is as the animal food that does not contain cholesterol; Because it is of high nutritive value, is rich in the various active composition; To the desirable invigorant that is regarded as improving the health by people, modern science shows that to the sea cucumber The Chemical Constituents sea cucumber body contains multiple bioactivator such as collagen, acid mucopolysaccharide and saponin(e etc. in China one, and pharmacologically active very extensively.The major part of beche-de-mer glue derives from body wall, and in leaching process, it is the major part that eats that stickiness polysaccharide wherein and saponin(e also dissolve in the body wall that is extracted out sea cucumber in the sea cucumber glue thereupon; And be rich in collagen in the body wall; Collagen is distributed widely in the metazoan body, has good biological characteristics and weak antigenicity, after specially treated; Can be used for pharmaceutical sanitary fields such as burn, wound, beauty treatment, orthopedic, organization restoration, surface of a wound hemostasis; The sea cucumber acid mucopolysaccharide also is the distinctive important component of sea cucumber body wall, so beche-de-mer can be comprehensively apace for human body supplements the nutrients, and enhance immunity power.Along with growth in the living standard, sea cucumber has become the daily nutritional health food of people.At present, China's sea cucumber output cumulative year after year, the sea cucumber industry is just flourish.For a long time, sea cucumber usually is prepared to the product of forms such as sea cucumber goods, capsule, oral liquid, and a large number of nutrients and bioactive ingredients run off in the preparation process.
Summary of the invention
Technical problem to be solved by this invention is to be to provide the full nutrition of a kind of sea cucumber to concentrate beche-de-mer glue; Compare with the beche-de-mer products of forms such as existing sea cucumber goods, capsule, oral liquid; It has kept a large amount of nutriments and the bioactive ingredients in the sea cucumber; Overcome the shortcoming that existing cucumber product can not fully utilize sea cucumber albumen, nutritive value is higher, can supplement the nutrients for human body fast comprehensively.
In order to solve the problems of the technologies described above, the invention provides a kind of sea cucumber glue, it comprises: weight ratio is that 5%～70% collagen, weight ratio are that 1%～15% sea cucumber mucopolysaccharide and weight ratio are 5%～80% water.
Wherein, said sea cucumber glue is prepared from through following method, comprise,
The first step is carried out preliminary treatment to sea cucumber;
Second step, pretreated sea cucumber is carried glue, obtain the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step was obtained removes heavy metal;
In the 4th step, the sea cucumber glue that the 3rd step was removed behind the heavy metal concentrates and moulding.
Wherein, in the said first step,, dried sea-cucumber is carried out the rehydration preliminary treatment if pretreated sea cucumber is a dried sea-cucumber; If pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment.
Wherein, in said second step, the sea cucumber that the first step is pretreated adds appropriate amount of deionized water; Stir homogenate, under uniform temperature and pressure, carry glue, it is centrifugal to carry glued bundle back; Separation of supernatant adds deionized water with the deposition of proposing centrifugal acquisition behind the glue and endures glue once more, carries glue so repeatedly several times; Merge and carry the supernatant that glue obtains at every turn, obtain the sea cucumber glue.
Wherein, adopt adsorbent to remove the sea cucumber glue that obtains in said second step in said the 3rd step, said adsorbent can be macroreticular resin.
For solving the problems of the technologies described above, the present invention also provides the preparation method of above-mentioned sea cucumber glue, and it comprises,
The first step is carried out preliminary treatment to sea cucumber;
Second step, pretreated sea cucumber is carried glue, obtain the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step was obtained removes heavy metal;
In the 4th step, the sea cucumber glue that the 3rd step was removed behind the heavy metal concentrates and moulding.
Wherein, in the said first step, if pretreated sea cucumber is a dried sea-cucumber; Dried sea-cucumber is carried out the rehydration preliminary treatment,, preliminary treatment is dissected, cleaned to bright sea cucumber if pretreated sea cucumber is bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment;
In said second step, the sea cucumber that the first step is pretreated adds deionized water, stirs homogenate; Under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, carried glue 0.5 hour～4 hours, and obtained slurries, the deionized water of adding and sea cucumber mass ratio are 1: 0.4～1: 20; It is centrifugal to carry glued bundle back; Separation of supernatant adds deionized water with the deposition of proposing centrifugal acquisition behind the glue and endures glue once more, the said deionized water of adding with to add the deionized water quality first time identical; Carry glue so repeatedly 2～5 times; Each all is under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, to carry glue 0.5 hour～4 hours, merges to carry the supernatant that glue obtains at every turn, obtains the sea cucumber glue;
In said the 3rd step; Adopt macroreticular resin to remove the heavy metal in the sea cucumber glue that obtains in said second step as adsorbent; The adsorbent use amount is 0.05%～10% mass percent of the sea cucumber glue of second step acquisition; Adsorption temp is 30～90 ℃, and adsorption time is 1 hour～6 hours;
In said the 4th step; Different conditions type according to needed sea cucumber glue can adopt different steps; If obtain sea cucumber jelly, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, and congealing at 4～10 ℃ obtains the sea cucumber jelly; If acquisition is the sea cucumber blob of viscose; The sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, congeals, and stripping and slicing also finally is dried to the 15% following mass percent that moisture accounts for the sea cucumber blob of viscose product of final acquisition at 10～20 ℃.
For solving the problems of the technologies described above, the present invention also provides a kind of medicine or health products, and it comprises above-mentioned sea cucumber glue.
For solving the problems of the technologies described above, the present invention also provides the preparation method of above-mentioned medicine or health products, and it comprises the preparation method of above-mentioned sea cucumber glue.
For solving the problems of the technologies described above, the present invention also provides the application of preparation method in preparation health products or medicine of above-mentioned sea cucumber glue or above-mentioned sea cucumber glue.
Beneficial effect of the present invention:
The invention provides the full nutrition of a kind of sea cucumber and concentrate beche-de-mer glue and preparation method thereof; Compare with the beche-de-mer products of forms such as existing sea cucumber goods, capsule, oral liquid; It has kept a large amount of nutriments and the bioactive ingredients in the sea cucumber; Overcome the shortcoming that existing cucumber product can not fully utilize sea cucumber albumen, nutritive value is higher, can supplement the nutrients for human body fast comprehensively.
The specific embodiment
The invention provides a kind of sea cucumber glue, it comprises: weight ratio is that the collagen of 5%-70%, sea cucumber mucopolysaccharide and the weight ratio that weight ratio is 1%-15% are 5%-80% water.
The state of said sea cucumber glue can be gelatin or blob of viscose shape.
The present invention also provides the preparation method of above-mentioned sea cucumber glue, and it comprises:
The first step is carried out preliminary treatment to sea cucumber;
Second step, pretreated sea cucumber is carried glue, obtain the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step was obtained removes heavy metal;
In the 4th step, the sea cucumber glue that the 3rd step was removed behind the heavy metal concentrates and moulding.
Further, said preparation method only is made up of above-mentioned steps.
Sea cucumber in the said first step can be various sea cucumbers, and like dried sea-cucumber, bright sea cucumber or salt sea cucumber further are preferably one or more mixing in stichopus japonicus, plum blossom ginseng, Virgin's milk ginseng, white ginseng, Haiti melon or the beche-de-mer in East China Sea.
The said first step further comprises: if pretreated sea cucumber is a dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment; If pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment.
Said second step comprises that further the sea cucumber that the first step is pretreated adds appropriate amount of deionized water, stirs homogenate, under uniform temperature and pressure, carries glue; It is centrifugal to carry glued bundle back; Separation of supernatant adds deionized water with the deposition of proposing centrifugal acquisition behind the glue and endures glue once more, carries glue so repeatedly several times; Merge and carry the supernatant that glue obtains at every turn, obtain the sea cucumber glue.
Carrying in the glue process for the first time, the said deionized water of adding and the mass ratio of sea cucumber be preferably 1: 0.4～1: 20, and under the certain situation of the temperature of carrying glue, pH value, time, slurries liquid coefficient is big more, and plastic emitting is many more, and recovery rate is big more, otherwise then less.From considering that economically slurries liquid coefficient is excessive, the glue content of extraction is low; When glue concentrates, can consume more steam and electric power, also reduce equipment capacity; Slurries liquid coefficient will be limited in the above-mentioned scope; Can make the production optimization, at every turn carrying in the glue process subsequently, the said deionized water of adding is with to add for the first time the deionized water quality identical.
Because it is high more to put forward the glue temperature, it is fast more that sea cucumber glue is separated speed, can enhance productivity, but secondary hydrolysis speed is fast, reduces sea cucumber colloid amount, and the service life of reducing equipment; Sea cucumber glue denaturation temperature is about 40 ℃, so the said temperature of carrying glue is preferably 40 ℃～130 ℃.Method for distilling can be divided into normal pressure and high pressure, and according to the device-restrictive of actual production and the difference of method for distilling, its corresponding pressure limit is 0.1MPa～0.3MPa.After carrying glue temperature and liquid coefficient and confirming; The time of extracting with put gum concentration and be directly proportional; Concentration is high, and the molecule degree that secondary hydrolysis takes place of being heated is also big more, thus the time carry at every turn the time of glue be preferably 0.5 hour～4 hours; The quality of recovery rate and glue be can guarantee like this, more steaminess and electric power are unlikely to when concentrated, to consume again.Collagen untwists to slowly carrying out in putting forward the glue process, in order to limit the gelatin molecule that is present in the sea cucumber colloidality secondary hydrolysis takes place too much, so the concentration of the gelatin in gelatin solution reaches one regularly; Just from carry colloid system, emit, so just add water and carry out the second time, carry glue for the third time; So carry out; Till the gelatin that the collagen in the raw material is changed into almost all extracts, take all factors into consideration energy resource consumption again, be preferably 2～5 times so carry the number of times of glue.
Adopt adsorbent to remove the sea cucumber glue that obtains in said second step in said the 3rd step; Said adsorbent can be macroreticular resin; Further be preferably the 734 type macroreticular resins of Tianjin Kermel Chemical Reagent Co., Ltd., the adsorbent use amount is 0.05%～10% (mass percent) of the sea cucumber glue of second step acquisition, and adsorption temp is preferably 30～90 ℃; Adsorption time is preferably 1 hour～and 6 hours, best in this condition adsorption capacity.
In said the 4th step; Different conditions type according to needed sea cucumber glue can adopt different steps; If obtain sea cucumber jelly, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, and congealing at 4～10 ℃ obtains the sea cucumber jelly; If acquisition is the sea cucumber blob of viscose; The sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, congeals, and stripping and slicing also finally is dried to the 15% following mass percent that moisture accounts for the sea cucumber blob of viscose product of final acquisition at 10～20 ℃.
Comprise in the said sea cucumber glue that weight ratio is that 5%～70% collagen, weight ratio are that 1%～15% sea cucumber mucopolysaccharide and weight ratio are 5%～80% water.
The present invention also provides a kind of sea cucumber glue, and said sea cucumber glue is prepared from through following method, and it comprises,
The first step is carried out preliminary treatment to sea cucumber;
Second step, pretreated sea cucumber is carried glue, obtain the sea cucumber glue;
The 3rd step; Adopt macroreticular resin to remove the heavy metal in the sea cucumber glue that obtains in said second step as adsorbent; The adsorbent use amount is 0.05%～10% mass percent of the sea cucumber glue of second step acquisition, and adsorption temp is 30～90 ℃, and adsorption time is 1 hour～6 hours;
In the 4th step, the sea cucumber glue that the 3rd step was removed behind the heavy metal concentrates and moulding;
The said first step further comprises, if pretreated sea cucumber is a dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment; If pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment;
Said second step comprises that further the sea cucumber that the first step is pretreated adds appropriate amount of deionized water, stirs homogenate; Under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, carried glue 0.5 hour～4 hours, and obtained slurries, the deionized water of adding and sea cucumber mass ratio are 1: 0.4～1: 20; It is centrifugal to carry glued bundle back; Separation of supernatant adds deionized water with the deposition of proposing centrifugal acquisition behind the glue and endures glue once more, and the deionized water of adding is identical with the deionized water quality of adding for the first time; Carry glue so repeatedly 2～5 times; Each all is under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, to carry glue 0.5 hour～4 hours, merges to carry the supernatant that glue obtains at every turn, obtains the sea cucumber glue;
Said the 4th step further comprises; Different conditions type according to needed sea cucumber glue can adopt different steps; If obtain sea cucumber jelly, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, and congealing through 4～10 ℃ obtains the sea cucumber jelly again; If acquisition is the sea cucumber blob of viscose; The sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, congeals, and stripping and slicing also finally is dried to below 15% mass percent of sea cucumber blob of viscose product that moisture accounts for final acquisition at 10 ℃～20 ℃.
Further, said preparation method only is made up of above-mentioned steps.
Sea cucumber in the said first step can be various sea cucumbers, and like dried sea-cucumber, bright sea cucumber or salt sea cucumber further are preferably one or more mixing in stichopus japonicus, plum blossom ginseng, Virgin's milk ginseng, white ginseng, Haiti melon or the beche-de-mer in East China Sea.
Comprise in the said sea cucumber glue that weight ratio is that 5%～70% collagen, weight ratio are that 1%～15% sea cucumber mucopolysaccharide and weight ratio are 5%～80% water.
The present invention also provides a kind of health products, and it comprises the sea cucumber glue that above-mentioned sea cucumber glue or above-mentioned preparation method obtain.
The present invention also provides a kind of health products, wherein, the sea cucumber glue that above-mentioned sea cucumber glue or above-mentioned preparation method obtain in said health products as unique health-care components.
The present invention also provides the preparation method of above-mentioned health products, it comprise among the preparation method of above-mentioned sea cucumber glue institute in steps.
The present invention also provides a kind of medicine, and it comprises the sea cucumber glue that above-mentioned sea cucumber glue or above-mentioned preparation method obtain.
The present invention also provides a kind of medicine, wherein, the sea cucumber glue that above-mentioned sea cucumber glue or above-mentioned preparation method obtain in said medicine as unique active component.
The present invention also provides the preparation method of above-mentioned medicine, it comprise among the preparation method of above-mentioned sea cucumber glue institute in steps.
The present invention also provides the preparation method and the application of above-mentioned sea cucumber glue in preparation health products or medicine of above-mentioned sea cucumber glue.
Below adopt embodiment to specify embodiment of the present invention, how the application technology means solve technical problem to the present invention whereby, and the implementation procedure of reaching technique effect can make much of and implement according to this.
1. the mensuration of collagen content: adopt AAS
(1) preparation of L-hydroxyproline standard liquid: accurately take by weighing L-hydroxyproline 100mg, add in the 0.01mol/L hydrochloric acid and dissolve, be settled to 100ml, obtain the reference substance standard liquid of 1mg/mL.。
(2) drafting of hydroxyproline calibration curve:
Draw hydroxyproline storage liquid 10ml in the 100ml volumetric flask, to 100ml (face and use preceding configuration), concentration is 100ug/ml with the distilled water constant volume.Draw hydroxyproline standard operation liquid 3.0,6.0,9.0,12.0,15.0ml, be settled to 100ml with distilled water respectively, its concentration is respectively 1.0,2.0,3.0,4.0,5.0ug/ml.With distilled water is blank, draws above-mentioned titer 1ml respectively, adds 1ml citrate buffer solution (Ph 6.0) and 1ml toluene-sodium-sulfonchloramide solution at room temperature (25 ℃) oxidation 10min; Add perchloric acid 1ml and place 10min, add Paradimethylaminobenzaldehyde 1ml, 65 ℃ of water-bath colour developing 20min; Cooling back is the survey absorbance at 560nm place, is abscissa with the concentration (μ g/mL) of L-hydroxyproline titer, is that ordinate is done calibration curve with the absorbance A; Regression equation is y=0.0245x+0.0553, R2=0.9942
(3) mensuration of collagen content: above-mentioned pretreated sample is got 1ml operate, survey absorbance at the 560nm place according to the method for above-mentioned production standard curve.According to L-hydroxyproline content calibration curve, draw L-hydroxyproline content in the sea cucumber.Hydroxyproline content accounts for 7% of collagen.Experimental result in this experiment is the mean value of 5 parallel determinations.
2. polyoses content analysis: adopt the column front derivation high performance liquid chromatography
(1) preparation of monose standard items derivative: accurately draw the solution of Man, GlcN, GlcUA, GalUA, Gal, Ara, 8 kinds of monose standard items of Fuc and internal standard compound Lac preparation written treaty 0.36/L, draw 50 μ L respectively and add in the test tube, add 450 μ L 0.5mol/L 1-phenyl-3-methyl-5-pyrazolones ketone (PMP) methanol solution and isopyknic 0.3mol/LNaOH solution then; The vortex mixing; In 70 ℃ of water-bath 30min, take out and be cooled to room temperature, neutralize with 450 μ L 0.3mol/L HCl solution; Add the 1mL chloroform extraction; Fully vibration is drawn lower floor's organic facies, triplicate.The upper strata water is crossed film, get 10 μ L sample introductions.
(2) sample preparation: (± 0.05g) sea cucumber glue, the amount that adds papain is the 100mg/g sample, behind 60 ℃ of water bath with thermostatic control oscillating reactions 24h enzymolysis liquid all is transferred to the 50mL centrifuge tube, the centrifugal 10min of 9000r/min accurately to take by weighing 0.5g～1.0g.Discard deposition, supernatant is transferred in the 100mL beaker that to add absolute ethyl alcohol to ethanol volume fraction be 60%, and to add sodium chloride to sodium chloride concentration be 0.5mol/L; Ultrasonicly dissolve fully to sodium chloride, be transferred to centrifuge tube after leaving standstill 12h in 4 ℃, with the centrifugal 10min of 9000r/min; Abandoning supernatant; Deposition is transferred to the 10mL volumetric flask after with the 5mL dissolved in distilled water, is settled to scale, promptly gets sea cucumber polysaccharide solution.Get 1.0mL in 5mL ampere bottle, add 1.0mL 4mol/L trifluoroacetic acid solution, fill the nitrogen tube sealing; In 110 ℃ of following hydrolysis 8h; 70 ℃ of following nitrogen dry up, and with 2mL ultra-pure water ultrasonic dissolution residue, regulate the monose hydrolyzate with the 0.3mol/L sodium hydroxide solution and to neutrality, are transferred to the 5mL volumetric flask; Be settled to scale, promptly get the sea cucumber polysaccharide hydrolyzate.Column front derivation and (1) are together.
(3) chromatographic condition: chromatographic condition: Agilent 1100 high performance liquid chromatographs, collocation ZORBAXEclipse XDB-C18 splitter (4.6mm * 150mm, 5 μ m), UV-detector (254nm); Flow velocity: 1.0mL/min; Column temperature: 25 ℃; Mobile phase A: 15% acetonitrile+0.05mol/L phosphate buffer (KH2PO4-NaOH, pH6.9); Mobile phase B: 40% acetonitrile+0.05mol/L phosphate buffer (KH2PO4-NaOH, pH6.9); Time gradient: 0 → 10 → 30min; Concentration gradient: 0 → 8% → 20% Mobile phase B; Sampling volume: 10 μ L.
Embodiment 1: the making of beche-de-mer jelly:
Take by weighing dried sea-cucumber 2Kg, add in the 2Kg deionized water with the moderate heat heating, boiled after the boiling 30 minutes again, well-done sea cucumber is rehydration 48 hours at normal temperatures.Waterishlogged Stichopus japonicus that rehydration is good adds the deionized water of 2kg and use refiner homogenate, and acquisition 4Kg sea cucumber homogenate adds the 8Kg deionized water again and under 80 ℃, 0.1MP, carried glue 30 minutes; The slurries that obtain are centrifugal, get supernatant, with the deposition homogenate once more of centrifugal acquisition; Add the 10Kg deionized water and endure glue once more; Carry glue so repeatedly 3 times, merge each supernatant that obtains, obtain the sea cucumber glue of 30Kg.In the sea cucumber glue that obtains, add and account for the 734 type macroreticular resins that sea cucumber glue mass fraction is Tianjin Kermel Chemical Reagent Co., Ltd. of 0.1%, under 40 ℃, removed heavy metal 6 hours, the time of removing is 2 hours; It is 30% that the sea cucumber glue that will remove heavy metal again is evaporated to gelatin concentration; Be moisture account for the sea cucumber glue 70%, congeal again, obtain sea cucumber jelly product; Its collagen content is 5.9%, and polyoses content is 1.1%.
Embodiment 2: the making of beche-de-mer blob of viscose:
Take by weighing bright sea cucumber 2Kg, sea cucumber is gilled and the lime mouth, the deionized water that adds 2kg behind the clean silt is used refiner homogenate, obtains sea cucumber homogenate 4Kg.In the sea cucumber homogenate, add the 8Kg deionized water, under 110 ℃, 0.2MP, carried glue 0.5 hour, the slurries that obtain are centrifugal, get supernatant, the deposition homogenate once more with centrifugal acquisition adds the 10Kg deionized water and endures glue once more.Carry glue so repeatedly 3 times, merge each supernatant that obtains, obtain 30Kg sea cucumber glue.Add in the sea cucumber glue that obtains that to account for sea cucumber glue mass fraction be 1.0% macroreticular resin, under 40 ℃, removed heavy metal 6 hours.The sea cucumber glue that will remove heavy metal again is evaporated to moisture and accounts for 60% of sea cucumber glue; Carrying out condensation again, is 10% at 20 ℃ of following cold air dryings to moisture, packing; Sterilization; Obtain sea cucumber blob of viscose product, the percentage composition of its collagen is 35.0%, and the polysaccharide percentage composition is 7.5%.
This intellectual property of primary enforcement that all are above-mentioned is not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any professional and technical personnel of being familiar with possibly utilize the technology contents of above-mentioned announcement to change or be modified as the equivalent embodiment of equivalent variations.But everyly do not break away from technical scheme content of the present invention, to any simple modification, equivalent variations and remodeling that above embodiment did, still belong to the protection domain of technical scheme of the present invention according to technical spirit of the present invention.
Claims (10)
1. a sea cucumber glue is characterized in that, comprising: weight ratio is that 5%～70% collagen, weight ratio are that 1%～15% sea cucumber mucopolysaccharide and weight ratio are 5%～80% water.
2. sea cucumber glue as claimed in claim 1 is characterized in that, said sea cucumber glue is prepared from through following method, comprising:
The first step is carried out preliminary treatment to sea cucumber;
Second step, pretreated sea cucumber is carried glue, obtain the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step was obtained removes heavy metal;
In the 4th step, the sea cucumber glue that the 3rd step was removed behind the heavy metal concentrates and moulding.
3. sea cucumber glue as claimed in claim 2 is characterized in that: in the said first step, if pretreated sea cucumber is a dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment; If pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment.
4. like claim 2 or 3 described sea cucumber glue, it is characterized in that: in said second step, the sea cucumber that the first step is pretreated adds appropriate amount of deionized water; Stir homogenate, under uniform temperature and pressure, carry glue, it is centrifugal to carry glued bundle back; Separation of supernatant adds deionized water with the deposition of proposing centrifugal acquisition behind the glue and endures glue once more, carries glue so repeatedly several times; Merge and carry the supernatant that glue obtains at every turn, obtain the sea cucumber glue.
5. like the described sea cucumber glue of claim 2 to 4, it is characterized in that: adopt adsorbent to remove the sea cucumber glue that obtains in said second step in said the 3rd step, said adsorbent can be macroreticular resin.
6. like the preparation method of the sea cucumber glue of claim 1 to 5, it is characterized in that, comprising:
The first step is carried out preliminary treatment to sea cucumber;
Second step, pretreated sea cucumber is carried glue, obtain the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step was obtained removes heavy metal;
In the 4th step, the sea cucumber glue that the 3rd step was removed behind the heavy metal concentrates and moulding.
7. preparation method as claimed in claim 6 is characterized in that:
In the said first step,, dried sea-cucumber is carried out the rehydration preliminary treatment if pretreated sea cucumber is a dried sea-cucumber; If pretreated sea cucumber is bright sea cucumber; Preliminary treatment is dissected, cleaned to bright sea cucumber, if pretreated be the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment;
In said second step, the sea cucumber homogenate that the first step is pretreated adds deionized water; Under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, carried glue 0.5 hour～4 hours, and obtained slurries, the deionized water of adding and sea cucumber mass ratio are 1: 0.4～1: 20; It is centrifugal to carry glued bundle back; Separation of supernatant adds deionized water with the deposition of proposing centrifugal acquisition behind the glue and endures glue once more, and the said deionized water of adding is with identical with the deionized water quality of adding for the first time; Carry glue so repeatedly 2～5 times; Each all is under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, to carry glue 0.5 hour～4 hours, merges to carry the supernatant that glue obtains at every turn, obtains the sea cucumber glue;
In said the 3rd step; Adopt macroreticular resin to remove the heavy metal in the sea cucumber glue that obtains in said second step as adsorbent; The adsorbent use amount is 0.05%～10% mass percent of the sea cucumber glue of second step acquisition; Adsorption temp is 30～90 ℃, and adsorption time is 1 hour～6 hours;
In said the 4th step; Different conditions type according to needed sea cucumber glue can adopt different steps; If obtain sea cucumber jelly, then the 3rd step was removed 60%～90% mass percent that the concentrated moisture of sea cucumber glue behind the heavy metal accounts for the sea cucumber glue, again through 4～10 ℃ of acquisition sea cucumber jelly that congeal; If acquisition is the sea cucumber blob of viscose; Then the 3rd step was removed 60%～90% mass percent that the concentrated moisture of sea cucumber glue behind the heavy metal accounts for the sea cucumber glue, congeal, stripping and slicing and final being dried under 10 ℃～20 ℃ below 15% mass percent of sea cucumber blob of viscose product that moisture accounts for final acquisition.
8. medicine or health products is characterized in that: comprise the described sea cucumber glue of claim 1 to 5.
9. the preparation method of described medicine of claim 8 or health products is characterized in that: the preparation method who comprises claim 6 or 7 described sea cucumber glue.
10. the application of the preparation method of each described sea cucumber glue of claim 1 to 5 or claim 6 or 7 described sea cucumber glue in preparation health products and medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104112801A CN102488251B (en) | 2011-12-12 | 2011-12-12 | Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104112801A CN102488251B (en) | 2011-12-12 | 2011-12-12 | Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102488251A true CN102488251A (en) | 2012-06-13 |
| CN102488251B CN102488251B (en) | 2013-04-03 |
Family
ID=46180169
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104112801A Expired - Fee Related CN102488251B (en) | 2011-12-12 | 2011-12-12 | Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102488251B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070419A (en) * | 2013-01-22 | 2013-05-01 | 大连湘蓝生物科技有限公司 | Sea cucumber gel and production method thereof |
| CN104585760A (en) * | 2015-01-15 | 2015-05-06 | 大连卓尔高科技有限公司 | Process for processing sea cucumber decoction |
| CN107334097A (en) * | 2017-08-04 | 2017-11-10 | 青岛康尔高科实业有限公司 | A kind of preparation method and application of sea cucumber extraction cream |
| CN116746668A (en) * | 2023-07-04 | 2023-09-15 | 中国海洋大学 | Compound fish glue for resisting iron deficiency anemia and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1277205A (en) * | 2000-05-17 | 2000-12-20 | 徐绥绪 | Extraction of gensenoside |
| CN1440985A (en) * | 2003-03-03 | 2003-09-10 | 威海健方医药研究所 | Holothuroids polysaccharide preparing method |
| CN1871945A (en) * | 2005-06-01 | 2006-12-06 | 王伟成 | Method for distilling freeze-dried instant powder from active substance of sea cucumber |
| CN101965973A (en) * | 2010-11-02 | 2011-02-09 | 大连海晏堂生物有限公司 | Fresh lotus root and sea cucumber gum food and preparation method thereof |
-
2011
- 2011-12-12 CN CN2011104112801A patent/CN102488251B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1277205A (en) * | 2000-05-17 | 2000-12-20 | 徐绥绪 | Extraction of gensenoside |
| CN1440985A (en) * | 2003-03-03 | 2003-09-10 | 威海健方医药研究所 | Holothuroids polysaccharide preparing method |
| CN1871945A (en) * | 2005-06-01 | 2006-12-06 | 王伟成 | Method for distilling freeze-dried instant powder from active substance of sea cucumber |
| CN101965973A (en) * | 2010-11-02 | 2011-02-09 | 大连海晏堂生物有限公司 | Fresh lotus root and sea cucumber gum food and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070419A (en) * | 2013-01-22 | 2013-05-01 | 大连湘蓝生物科技有限公司 | Sea cucumber gel and production method thereof |
| CN103070419B (en) * | 2013-01-22 | 2015-04-01 | 大连湘蓝生物科技有限公司 | Sea cucumber gel and production method thereof |
| CN104585760A (en) * | 2015-01-15 | 2015-05-06 | 大连卓尔高科技有限公司 | Process for processing sea cucumber decoction |
| CN107334097A (en) * | 2017-08-04 | 2017-11-10 | 青岛康尔高科实业有限公司 | A kind of preparation method and application of sea cucumber extraction cream |
| CN116746668A (en) * | 2023-07-04 | 2023-09-15 | 中国海洋大学 | Compound fish glue for resisting iron deficiency anemia and preparation method thereof |
| CN116746668B (en) * | 2023-07-04 | 2024-02-09 | 中国海洋大学 | Compound fish glue and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102488251B (en) | 2013-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102488251B (en) | Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin | |
| CN103540142B (en) | A kind of Edible squid skin gelatin composite film and preparation method thereof | |
| CN102038261B (en) | Method for preparing tree peony beverage | |
| CN103598400B (en) | A kind of extracting method of aquatic collagen protein | |
| CN105087729A (en) | Tuna bone collagen peptide preparation method | |
| CN101874632B (en) | Preparation method of collagen deer bone calcium capsules | |
| CN106047975A (en) | Mussel protein peptide extraction method | |
| CN103222610B (en) | Chrysanthemum morifolium Ramat liquid extractive as well as preparation method and application thereof | |
| CN105399766A (en) | Duck egg yolk lecithin extracting and refining method | |
| CN104489796A (en) | Honey and fruit juice beverage preparation method | |
| CN102334676B (en) | Method for preparing collagen chelated calcium | |
| CN109497368A (en) | A kind of functional beverage and preparation method thereof containing Rosa roxburghii Tratt | |
| CN104642715A (en) | Method for extracting rice germ protein | |
| CN105342950A (en) | Skin-care lotion containing ewe placenta and preparation method thereof | |
| CN104116087A (en) | Preparation process of scale jelly | |
| CN106480145A (en) | A kind of extracting method of small molecule oyster polypeptide | |
| CN103087638B (en) | Low-freezing-point low-freezing-strength gelatin and preparation method thereof | |
| CN103141838A (en) | Preparation method and application of female calcium supplementing product | |
| CN106636267A (en) | Extracting method of small-molecular sea cucumber-oyster polypeptide | |
| CN105029554A (en) | Self-prepared health beverage and production method thereof | |
| CN109929022A (en) | A kind of preparation method of catfish protamine sulfate | |
| CN104586894A (en) | Method for extracting amino acids from eggs | |
| CN103535824A (en) | Pure natural desert green alga beverage as well as preparation method thereof | |
| CN104042622B (en) | A kind of snow chrysanthemum polyoses oral liquid and production method thereof | |
| CN109757635A (en) | A kind of gold marrow decocts beverage and its manufacture craft |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130403 Termination date: 20161212 |