CN102488251B - Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin - Google Patents
Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin Download PDFInfo
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- CN102488251B CN102488251B CN2011104112801A CN201110411280A CN102488251B CN 102488251 B CN102488251 B CN 102488251B CN 2011104112801 A CN2011104112801 A CN 2011104112801A CN 201110411280 A CN201110411280 A CN 201110411280A CN 102488251 B CN102488251 B CN 102488251B
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Abstract
The invention provides a sea cucumber gelatin, which is prepared by the following steps of: 1, preprocessing sea cucumbers; 2, extracting a gelatin from the preprocessed sea cucumbers to obtain a sea cucumber gelatin liquor; 3, removing heavy metals from the sea cucumber gelatin liquor obtained in the step 2; and 4, concentrating the sea cucumber gelatin liquor from which the heavy metals are removed, and molding. The invention provides a sea cucumber total nutrient concentrated edible sea cucumber gelatin and a preparation method thereof. Compared with the conventional sea cucumber dried products, capsules, oral solutions and other sea cucumber products, the sea cucumber gelatin maintains a large number of nutrients substances and bioactive components in the sea cucumbers, overcomes the defect that the conventional sea cucumber products cannot fully utilize sea cucumber proteins, has higher nutritional value and can comprehensively and quickly supplement nutrients for a human body.
Description
Technical field
The present invention relates to a kind of cucumber product, relate in particular to a kind of beche-de-mer glue.
Background technology
Sea cucumber is one of the world's eight large treasures, it is as the animal food that does not contain cholesterol, because it is of high nutritive value, is rich in the various active composition, in China one to the desirable invigorant that is considered as improving the health by people, modern science contains various bioactivators such as collagen, acid mucopolysaccharide and saponin(e etc. to the sea cucumber body that studies show that of sea cucumber chemical composition, and pharmacologically active is very extensive.The major part of beche-de-mer glue derives from body wall, in leaching process, it is the major part that eats that stickiness polysaccharide wherein and saponin(e also dissolve in the body wall that is extracted out sea cucumber in the sea cucumber glue thereupon, and be rich in collagen in the body wall, collagen is distributed widely in the metazoan body, have good biological characteristics and weak antigenicity, after specially treated, can be used for burn, wound, beauty treatment, orthopedic, tissue repair, the pharmaceutical sanitary fields such as surface of a wound hemostasis, the sea cucumber acid mucopolysaccharide also is the distinctive important component of wall of sea cucumber Stichopus japonicus, so beche-de-mer can comprehensively rapidly for human body supplements the nutrients, strengthen immunity.Along with growth in the living standard, sea cucumber has become the daily nutritional health food of people.At present, China's sea cucumber output cumulative year after year, the sea cucumber industry is just flourish.For a long time, sea cucumber usually is prepared to the product of the forms such as sea cucumber goods, capsule, oral liquid, and a large number of nutrients and bioactive ingredients run off in preparation process.
Summary of the invention
Technical problem to be solved by this invention is to be to provide the full nutrition of a kind of sea cucumber to concentrate beche-de-mer glue, compare with the beche-de-mer products of the forms such as existing sea cucumber goods, capsule, oral liquid, it has kept a large amount of nutriments and the bioactive ingredients in the sea cucumber, overcome the shortcoming that existing cucumber product can not fully utilize sea cucumber albumen, nutritive value is higher, can supplement the nutrients for human body fast comprehensively.
In order to solve the problems of the technologies described above, the invention provides a kind of sea cucumber gum, it comprises: weight ratio is that 5%～70% collagen, weight ratio are that 1%～15% sea cucumber mucopolysaccharide and weight ratio are 5%～80% water.
Wherein, described sea cucumber gum is prepared from by the following method, comprise,
The first step is carried out preliminary treatment to sea cucumber;
Second step is carried glue with pretreated sea cucumber, obtains the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step is obtained removes heavy metal;
In the 4th step, the 3rd step was removed the concentrated and moulding of sea cucumber glue behind the heavy metal.
Wherein, in the described first step, if pretreated sea cucumber is dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment; If pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment.
Wherein, in described second step, the sea cucumber that the first step is pretreated adds appropriate amount of deionized water, stirs homogenate, carries glue under uniform temperature and pressure, carry behind the cementing bundle centrifugal, separation of supernatant adds deionized water with the precipitation of proposing centrifugal acquisition behind the glue and again endures glue, so repeatedly carries glue several times, merge and carry the supernatant that glue obtains at every turn, obtain the sea cucumber glue.
Wherein, adopt adsorbent to remove the sea cucumber glue that obtains in the described second step in described the 3rd step, described adsorbent can be macroreticular resin.
For solving the problems of the technologies described above, the present invention also provides the preparation method of above-mentioned sea cucumber gum, and it comprises,
The first step is carried out preliminary treatment to sea cucumber;
Second step is carried glue with pretreated sea cucumber, obtains the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step is obtained removes heavy metal;
In the 4th step, the 3rd step was removed the concentrated and moulding of sea cucumber glue behind the heavy metal.
Wherein, in the described first step, if pretreated sea cucumber is dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment, if pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber, if pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment;
In described second step, the sea cucumber that the first step is pretreated adds deionized water, stir homogenate, under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, carried glue 0.5 hour～4 hours, obtain slurries, the deionized water that adds is 1: 0.4～1: 20 with the quality of sea cucumber ratio, carry behind the cementing bundle centrifugal, separation of supernatant, the precipitation of proposing centrifugal acquisition behind the glue is added deionized water again endure glue, the described deionized water that adds with to add the deionized water quality first time identical, so repeatedly carry glue 2～5 times, each all is to carry glue 0.5 hour～4 hours under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, merges to carry the supernatant that glue obtains at every turn, obtains the sea cucumber glue;
In described the 3rd step, the employing macroreticular resin removes the heavy metal in the sea cucumber glue that obtains in the described second step as adsorbent, the adsorbent use amount is 0.05%～10% mass percent of the sea cucumber glue of second step acquisition, adsorption temp is 30～90 ℃, and adsorption time is 1 hour～6 hours;
In described the 4th step, different conditions type according to needed sea cucumber gum can adopt different steps, if obtain sea cucumber jelly, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, at 4～10 ℃ of acquisition sea cucumber jelly that congeal, if acquisition is the sea cucumber blob of viscose, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, congeal, stripping and slicing also finally is dried to the 15% following mass percent that moisture accounts for the sea cucumber blob of viscose product of final acquisition at 10～20 ℃.
For solving the problems of the technologies described above, the present invention also provides a kind of medicine or health products, and it comprises above-mentioned sea cucumber gum.
For solving the problems of the technologies described above, the present invention also provides the preparation method of above-mentioned medicine or health products, and it comprises the preparation method of above-mentioned sea cucumber gum.
For solving the problems of the technologies described above, the present invention also provides the application of preparation method in preparation health products or medicine of above-mentioned sea cucumber gum or above-mentioned sea cucumber gum.
Beneficial effect of the present invention:
The invention provides concentrated beche-de-mer glue of the full nutrition of a kind of sea cucumber and preparation method thereof, compare with the beche-de-mer products of the forms such as existing sea cucumber goods, capsule, oral liquid, it has kept a large amount of nutriments and the bioactive ingredients in the sea cucumber, overcome the shortcoming that existing cucumber product can not fully utilize sea cucumber albumen, nutritive value is higher, can supplement the nutrients for human body fast comprehensively.
The specific embodiment
The invention provides a kind of sea cucumber gum, it comprises: weight ratio is that the collagen of 5%-70%, sea cucumber mucopolysaccharide and the weight ratio that weight ratio is 1%-15% are 5%-80% water.
The state of described sea cucumber gum can be gelatin or blob of viscose shape.
The present invention also provides the preparation method of above-mentioned sea cucumber gum, and it comprises:
The first step is carried out preliminary treatment to sea cucumber;
Second step is carried glue with pretreated sea cucumber, obtains the sea cucumber glue;
In the 3rd step, the sea cucumber glue that second step is obtained removes heavy metal;
In the 4th step, the 3rd step was removed the concentrated and moulding of sea cucumber glue behind the heavy metal.
Further, described preparation method only is made of above-mentioned steps.
Sea cucumber in the described first step can be various sea cucumbers, and such as dried sea-cucumber, bright sea cucumber or salt sea cucumber further are preferably one or more mixing in stichopus japonicus, plum blossom ginseng, Virgin's milk ginseng, white ginseng, Haiti melon or the beche-de-mer in East China Sea.
The described first step further comprises: if pretreated sea cucumber is dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment; If pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment.
Described second step comprises that further the sea cucumber that the first step is pretreated adds appropriate amount of deionized water, stir homogenate, under uniform temperature and pressure, carry glue, carry behind the cementing bundle centrifugal, separation of supernatant adds deionized water with the precipitation of proposing centrifugal acquisition behind the glue and again endures glue, so repeatedly carries glue several times, merge and carry the supernatant that glue obtains at every turn, obtain the sea cucumber glue.
Carrying in the glue process for the first time, the described deionized water of adding and the mass ratio of sea cucumber be preferably 1: 0.4～1: 20, and in the certain situation of the temperature of carrying glue, pH value, time, slurries liquid coefficient is larger, and plastic emitting is more, and recovery rate is larger, otherwise then less.From considering economically, slurries liquid coefficient is excessive, the glue content that extracts is low, when glue concentrates, can consume more steam and electric power, also reduce equipment capacity, slurries liquid coefficient will be limited in the above-mentioned scope, can make operation optimization, at every turn carrying in the glue process subsequently, the described deionized water of adding is with to add for the first time the deionized water quality identical.
Because it is higher to put forward the glue temperature, the sea cucumber gum hydrolysis rate is faster, can enhance productivity, but secondary hydrolysis speed is fast, reduces the sea cucumber gum quality, and reduces the service life of equipment; The sea cucumber gum denaturation temperature is about 40 ℃, so the described temperature of carrying glue is preferably 40 ℃～130 ℃.Extracting method can be divided into normal pressure and high pressure, and according to the device-restrictive of actual production and the difference of extracting method, its corresponding pressure limit is 0.1MPa～0.3MPa.After carrying glue temperature and liquid parameter identification, the time of extracting with put gum concentration and be directly proportional, concentration is high, the molecule degree that secondary hydrolysis occurs of being heated is also larger, so the time carry at every turn the time of glue be preferably 0.5 hour～4 hours, the quality of recovery rate and glue be can guarantee like this, more steaminess and electric power are unlikely to again when concentrated, to consume.Collagen untwists as slowly carrying out in putting forward the glue process, secondary hydrolysis occurs too much in order to limit the gelatin molecule that is present in the sea cucumber colloidality, so the concentration of the gelatin in gelatin solution reaches one regularly, just from carry colloid system, emit, so just add water and carry out the second time, carry for the third time glue, so carry out, until till the gelatin that the collagen in the raw material is changed into almost all extracts, consider again energy resource consumption, be preferably 2～5 times so carry the number of times of glue.
Adopt adsorbent to remove the sea cucumber glue that obtains in the described second step in described the 3rd step, described adsorbent can be macroreticular resin, 734 type macroreticular resins of Tianjin Kermel Chemical Reagent Co., Ltd. more preferably, the adsorbent use amount is 0.05%～10% (mass percent) of the sea cucumber glue of second step acquisition, adsorption temp is preferably 30～90 ℃, adsorption time is preferably 1 hour～and 6 hours, best in this condition adsorption capacity.
In described the 4th step, different conditions type according to needed sea cucumber gum can adopt different steps, if obtain sea cucumber jelly, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, at 4～10 ℃ of acquisition sea cucumber jelly that congeal, if acquisition is the sea cucumber blob of viscose, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, congeal, stripping and slicing also finally is dried to the 15% following mass percent that moisture accounts for the sea cucumber blob of viscose product of final acquisition at 10～20 ℃.
Comprise in the described sea cucumber gum that weight ratio is that 5%～70% collagen, weight ratio are that 1%～15% sea cucumber mucopolysaccharide and weight ratio are 5%～80% water.
The present invention also provides a kind of sea cucumber gum, and described sea cucumber gum is prepared from by the following method, and it comprises,
The first step is carried out preliminary treatment to sea cucumber;
Second step is carried glue with pretreated sea cucumber, obtains the sea cucumber glue;
The 3rd step, the employing macroreticular resin removes the heavy metal in the sea cucumber glue that obtains in the described second step as adsorbent, the adsorbent use amount is 0.05%～10% mass percent of the sea cucumber glue of second step acquisition, and adsorption temp is 30～90 ℃, and adsorption time is 1 hour～6 hours;
In the 4th step, the 3rd step was removed the concentrated and moulding of sea cucumber glue behind the heavy metal;
The described first step further comprises, if pretreated sea cucumber is dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment; If pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber; If pretreated is the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment;
Described second step further comprises, the sea cucumber that the first step is pretreated adds appropriate amount of deionized water, stir homogenate, under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, carried glue 0.5 hour～4 hours, obtain slurries, the deionized water that adds is 1: 0.4～1: 20 with the quality of sea cucumber ratio, carry behind the cementing bundle centrifugal, separation of supernatant, the precipitation of proposing centrifugal acquisition behind the glue is added deionized water again endure glue, the deionized water that adds is identical with the deionized water quality that adds for the first time, so repeatedly carry glue 2～5 times, each all is to carry glue 0.5 hour～4 hours under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, merges to carry the supernatant that glue obtains at every turn, obtains the sea cucumber glue;
Described the 4th step further comprises, different conditions type according to needed sea cucumber gum can adopt different steps, if obtain sea cucumber jelly, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, congeal through 4～10 ℃ again and obtain sea cucumber jelly, if acquisition is the sea cucumber blob of viscose, the sea cucumber glue that then the 3rd step was removed behind the heavy metal is concentrated into 60%～90% mass percent that moisture accounts for the sea cucumber glue, congeal, stripping and slicing also finally is dried to below 15% mass percent of sea cucumber blob of viscose product that moisture accounts for final acquisition at 10 ℃～20 ℃.
Further, described preparation method only is made of above-mentioned steps.
Sea cucumber in the described first step can be various sea cucumbers, and such as dried sea-cucumber, bright sea cucumber or salt sea cucumber further are preferably one or more mixing in stichopus japonicus, plum blossom ginseng, Virgin's milk ginseng, white ginseng, Haiti melon or the beche-de-mer in East China Sea.
Comprise in the described sea cucumber gum that weight ratio is that 5%～70% collagen, weight ratio are that 1%～15% sea cucumber mucopolysaccharide and weight ratio are 5%～80% water.
The present invention also provides a kind of health products, and it comprises the sea cucumber gum that above-mentioned sea cucumber gum or above-mentioned preparation method obtain.
The present invention also provides a kind of health products, wherein, the sea cucumber gum that above-mentioned sea cucumber gum or above-mentioned preparation method obtain in described health products as unique health-care components.
The present invention also provides the preparation method of above-mentioned health products, it comprise among the preparation method of above-mentioned sea cucumber gum institute in steps.
The present invention also provides a kind of medicine, and it comprises the sea cucumber gum that above-mentioned sea cucumber gum or above-mentioned preparation method obtain.
The present invention also provides a kind of medicine, wherein, the sea cucumber gum that above-mentioned sea cucumber gum or above-mentioned preparation method obtain in described medicine as unique active component.
The present invention also provides the preparation method of above-mentioned medicine, it comprise among the preparation method of above-mentioned sea cucumber gum institute in steps.
The present invention also provides preparation method and the application of above-mentioned sea cucumber gum in preparation health products or medicine of above-mentioned sea cucumber gum.
Below adopt embodiment to describe embodiments of the present invention in detail, how the application technology means solve technical problem to the present invention whereby, and the implementation procedure of reaching technique effect can fully understand and implements according to this.
1. the mensuration of collagen content: adopt AAS
(1) preparation of L-hydroxyproline standard liquid: accurately take by weighing L-hydroxyproline 100mg, add in the 0.01mol/L hydrochloric acid and dissolve, be settled to 100ml, obtain the reference substance standard liquid of 1mg/mL.。
(2) drafting of hydroxyproline calibration curve:
Draw hydroxyproline storage liquid 10ml in the 100ml volumetric flask, to 100ml (before use configuration), concentration is 100ug/ml with the distilled water constant volume.Draw hydroxyproline standard operation liquid 3.0,6.0,9.0,12.0,15.0ml, be settled to 100ml with distilled water respectively, its concentration is respectively 1.0,2.0,3.0,4.0,5.0ug/ml.Take distilled water as blank, draw respectively above-mentioned titer 1ml, add 1ml citrate buffer solution (Ph 6.0) and 1ml toluene-sodium-sulfonchloramide solution at room temperature (25 ℃) oxidation 10min, add perchloric acid 1ml and place 10min, add Paradimethylaminobenzaldehyde 1ml, 65 ℃ of water-bath colour developing 20min, the rear absorbance of surveying at the 560nm place of cooling, take the concentration (μ g/mL) of L-hydroxyproline titer as abscissa, do calibration curve take absorbance A as ordinate, regression equation is y=0.0245x+0.0553, R2=0.9942
(3) mensuration of collagen content: above-mentioned pretreated sample is got 1ml operate according to the method for above-mentioned production standard curve, survey absorbance at the 560nm place.According to L-hydroxyproline content calibration curve, draw L-hydroxyproline content in the sea cucumber.Hydroxyproline content accounts for 7% of collagen.Experimental result in this experiment is the mean value of 5 parallel determinations.
2. polyoses content analysis: adopt the column front derivation high performance liquid chromatography
(1) preparation of monose standard items derivative: accurately draw Man, GlcN, GlcUA, GalUA, Gal, Ara, 8 kinds of monose standard items of Fuc and internal standard compound Lac preparation are into about the solution of 0.36/L, drawing respectively 50 μ L adds in the test tube, then add 450 μ L 0.5mol/L 1-phenyl-3-methyl-5-pyrazolones ketone (PMP) methanol solution and isopyknic 0.3mol/LNaOH solution, the vortex mixing, in 70 ℃ of water-bath 30min, taking-up is cooled to room temperature, neutralize with 450 μ L 0.3mol/L HCl solution, add the 1mL chloroform extraction, fully vibration, draw lower floor's organic phase, triplicate.The upper strata water is crossed film, get 10 μ L sample introductions.
(2) sample preparation: accurately take by weighing 0.5g～1.0g (± 0.05g) sea cucumber gum, the amount that adds papain is the 100mg/g sample, behind 60 ℃ of water bath with thermostatic control oscillating reactions 24h enzymolysis liquid all is transferred to the 50mL centrifuge tube, the centrifugal 10min of 9000r/min.Discard precipitation, supernatant is transferred in the 100mL beaker that to add absolute ethyl alcohol to volume fraction of ethanol be 60%, and adding sodium chloride to sodium chloride concentration is 0.5mol/L, ultrasonicly dissolve fully to sodium chloride, be transferred to centrifuge tube after leaving standstill 12h in 4 ℃, with the centrifugal 10min of 9000r/min, abandoning supernatant, precipitation is transferred to the 10mL volumetric flask after with the 5mL dissolved in distilled water, is settled to scale, namely gets sea cucumber polysaccharide solution.Get 1.0mL in 5mL ampere bottle, add 1.0mL 4mol/L trifluoroacetic acid solution, fill the nitrogen tube sealing, in 110 ℃ of lower hydrolysis 8h, 70 ℃ of lower nitrogen dry up, and with 2mL ultra-pure water ultrasonic dissolution residue, regulate the monose hydrolyzate with the 0.3mol/L sodium hydroxide solution and be transferred to the 5mL volumetric flask to neutrality, be settled to scale, namely get the sea cucumber polysaccharide hydrolyzate.Column front derivation and (1) are together.
(3) chromatographic condition: chromatographic condition: Agilent 1100 high performance liquid chromatographs, collocation ZORBAXEclipse XDB-C18 splitter (4.6mm * 150mm, 5 μ m), UV-detector (254nm); Flow velocity: 1.0mL/min; Column temperature: 25 ℃; Mobile phase A: 15% acetonitrile+0.05mol/L phosphate buffer (KH2PO4-NaOH, pH6.9); Mobile phase B: 40% acetonitrile+0.05mol/L phosphate buffer (KH2PO4-NaOH, pH6.9); Time gradient: 0 → 10 → 30min; Concentration gradient: 0 → 8% → 20% Mobile phase B; Sampling volume: 10 μ L.
Embodiment 1: the making of beche-de-mer jelly:
Take by weighing dried sea-cucumber 2Kg, add in the 2Kg deionized water with the moderate heat heating, boiled after the boiling 30 minutes again, well-done sea cucumber is rehydration 48 hours at normal temperatures.The waterishlogged Stichopus japonicus that rehydration is good adds the deionized water refiner homogenate of 2kg, obtain 4Kg sea cucumber homogenate, add again the 8Kg deionized water and under 80 ℃, 0.1MP, carried glue 30 minutes, the slurries that obtain are centrifugal, get supernatant, with the again homogenate of precipitation of centrifugal acquisition, add the 10Kg deionized water and again endure glue, so repeatedly carry glue 3 times, merge each supernatant that obtains, obtain the sea cucumber glue of 30Kg.In the sea cucumber glue that obtains, add and account for the 734 type macroreticular resins that sea cucumber glue mass fraction is Tianjin Kermel Chemical Reagent Co., Ltd. of 0.1%, under 40 ℃, removed heavy metal 6 hours, the time of removing is 2 hours, it is 30% that the sea cucumber glue that will remove again heavy metal is evaporated to gelatin concentration, be moisture account for the sea cucumber glue 70%, congeal again, obtain sea cucumber jelly product, its collagen content is 5.9%, and polyoses content is 1.1%.
Embodiment 2: the making of beche-de-mer blob of viscose:
Take by weighing bright sea cucumber 2Kg, sea cucumber is gilled and the lime mouth, add the deionized water refiner homogenate of 2kg behind the clean silt, obtain sea cucumber homogenate 4Kg.In the sea cucumber homogenate, add the 8Kg deionized water, under 110 ℃, 0.2MP, carried glue 0.5 hour, the slurries that obtain are centrifugal, get supernatant, the again homogenate of precipitation with centrifugal acquisition adds the 10Kg deionized water and again endures glue.So repeatedly carry glue 3 times, merge each supernatant that obtains, obtain 30Kg sea cucumber glue.Add in the sea cucumber glue that obtains that to account for sea cucumber glue mass fraction be 1.0% macroreticular resin, under 40 ℃, removed heavy metal 6 hours.The sea cucumber glue that will remove again heavy metal is evaporated to moisture and accounts for 60% of sea cucumber glue, carry out again condensation, be 10% at 20 ℃ of lower cold air dryings to moisture, packing, sterilization, obtain sea cucumber blob of viscose product, the percentage composition of its collagen is 35.0%, and the polysaccharide percentage composition is 7.5%.
This intellectual property of primary enforcement that all are above-mentioned is not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment does, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (1)
1. the preparation method of a sea cucumber gum is characterized in that, comprising:
The first step is carried out preliminary treatment to sea cucumber, when pretreated sea cucumber is dried sea-cucumber, dried sea-cucumber is carried out the rehydration preliminary treatment, when pretreated sea cucumber is bright sea cucumber, preliminary treatment is dissected, cleaned to bright sea cucumber, when being the salt sea cucumber, then the salt sea cucumber is carried out the desalination preliminary treatment when pretreated;
Second step, the sea cucumber homogenate that the first step is pretreated, add deionized water, under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, carried glue 0.5 hour～4 hours, obtain slurries, the deionized water that adds is 1: 0.4～1: 20 with the quality of sea cucumber ratio, carry behind the cementing bundle centrifugal, separation of supernatant, the precipitation of proposing centrifugal acquisition behind the glue is added deionized water again endure glue, the described deionized water that adds is identical with the deionized water quality that adds for the first time, so repeatedly carry glue 2～5 times, each all is to carry glue 0.5 hour～4 hours under 40 ℃～130 ℃ temperature and the pressure of 0.1MPa～0.3MPa, merges to carry the supernatant that glue obtains at every turn, obtains the sea cucumber glue;
The 3rd step, the employing macroreticular resin removes the heavy metal in the sea cucumber glue that obtains in the described second step as adsorbent, the adsorbent use amount is 0.05%～10% mass percent of the sea cucumber glue of second step acquisition, and adsorption temp is 30～90 ℃, and adsorption time is 1 hour～6 hours;
The 4th step, different conditions type according to needed sea cucumber gum adopts different steps, the 3rd step was removed 60%～90% mass percent that the concentrated moisture of sea cucumber glue behind the heavy metal accounts for the sea cucumber glue, congeal through 4～10 ℃ again and obtain sea cucumber jelly, perhaps removed 60%～90% mass percent that the concentrated moisture of sea cucumber glue behind the heavy metal accounts for the sea cucumber glue the 3rd step, congeal, stripping and slicing also finally accounts for below 15% mass percent of sea cucumber blob of viscose product of final acquisition being dried to moisture under 10 ℃～20 ℃.
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| CN104585760A (en) * | 2015-01-15 | 2015-05-06 | 大连卓尔高科技有限公司 | Process for processing sea cucumber decoction |
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| CN1112366C (en) * | 2000-05-17 | 2003-06-25 | 徐绥绪 | Extraction of gensenoside |
| CN1194018C (en) * | 2003-03-03 | 2005-03-23 | 威海健方医药研究所 | Holothuroids polysaccharide preparing method |
| CN1871945B (en) * | 2005-06-01 | 2010-05-26 | 王伟成 | Method for distilling freeze-dried instant powder from active substance of sea cucumber |
| CN101965973B (en) * | 2010-11-02 | 2012-07-04 | 大连海晏堂生物有限公司 | Fresh lotus root and sea cucumber gum food and preparation method thereof |
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2011
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