CN1194018C - Holothuroids polysaccharide preparing method - Google Patents
Holothuroids polysaccharide preparing method Download PDFInfo
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- CN1194018C CN1194018C CNB031119255A CN03111925A CN1194018C CN 1194018 C CN1194018 C CN 1194018C CN B031119255 A CNB031119255 A CN B031119255A CN 03111925 A CN03111925 A CN 03111925A CN 1194018 C CN1194018 C CN 1194018C
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Abstract
The present invention discloses a preparing method of sea cucumber polysaccharide, which depends on the function of autolytic enzyme of fresh sea cucumbers, and proper amount of natural organisms are used as degradation objects. A physical catalyzing method is combined and adopted, condensation is eliminated, any toxic and harmful chemical solvent is not added. The preparing method changes the original complicated technology method that chemical degradation objects are added for degradation, and after the degradation is carried out, the chemical degradation objects are removed; then, acid or alkali is added to regulate the pH value. The preparing method adopts the technology of direct ultrafiltration interception, post purification, washing with water, etc., so that the bioactivity the sea cucumber polysaccharide is kept completely. The preparing method has the advantages of simple production technology, low production cost, large amount of sea cucumber polysaccharide extracted in a production process, and high quantity, and simultaneously, the preparing method can also obtain remainders, most of which are proteins. The remaining liquid can be made into oral liquid with rich nutrition or food additives.
Description
Technical field
The present invention relates to a kind of extracting method of polysaccharide, specifically the preparation method of sea cucumber polysaccharide.
Background technology
Sea cucumber polysaccharide is that the live body sea cucumber from Echinodermata Holothuroidea Stichopodidae is extracted, separate, the polysaccharide of purifying, research sea cucumber polysaccharide and pharmacological action country the earliest thereof are the U.S., the U.S. in 1956 whole world cancer research association and pharmaceutical science research department have carried out a series of researchs to the antitumor action of trepang and Stichopus chloronotus (Brandt)., find the physiological function regulation and control of sea cucumber polysaccharide to human body, the optimum regime that earns a bare living has positive and important effect, afterwards, Japan and USSR (Union of Soviet Socialist Republics) have also been done the research work of many this respects, and find that sea cucumber polysaccharide is except antineoplastic action, also has anti-radiation, anticoagulation, effect such as antimycotic grade, in China, number of research projects has also been done to sea cucumber polysaccharide by many scientific research institutions of coastal provinces and cities, 1999, Shandong Province medical courses in general institute is successively at " Chinese marine drug " magazine, delivered the paper of " extraction of acidic polysaccharose and purifying research in the Stichopus variegatus (Sempen) " on " Shandong Traditional Chinese Medicine University's journal " the 23rd volume, all need to use hydrochloric acid in the leaching process of the sea cucumber polysaccharide of above-mentioned various countries research, sodium hydroxide, enzyme, Glacial acetic acid, acetone, ether, chemicals such as Vanadium Pentoxide in FLAKES, make in the sea cucumber polysaccharide that extracts chemical substance residual serious, and complex manufacturing, the production cost height, the sea cucumber polysaccharide amount of extracting is little, deficiencies such as matter difference.
Summary of the invention
The preparation method who the purpose of this invention is to provide the sea cucumber polysaccharide that a kind of production technique is simple, production cost is low, the sea cucumber polysaccharide quality of extraction is high.
The present invention can reach by following measure:
A kind of preparation method of sea cucumber polysaccharide, adopt following steps:
(1), with the fresh and alive sea cucumbers cleaning of cutting open the belly, fresh and alive sea cucumbers is cut open forward along belly from anus with scissors, take out intestines, clean with seawater, remove silt and the foreign material of sea cucumber,
(2), the sea cucumber of cleaning is weighed, add the wolfberry fruit of the 2%-5% of sea cucumber weight, add 2 times of water of sea cucumber weight, carry out fragmentation and defibrination, obtain sea cucumber milk, fragmentation can be adopted tissue mashing machine, and defibrination can adopt colloidal mill,
(3), add the water of 5 times of sea cucumber milks, heat to 40 ℃-45 ℃, and stir, constant temperature kept 2 hours to sea cucumber milk, also stir in the thermostatic process,
(4), in above-mentioned slurries, add the Chinese yam of the 1%-3% of sea cucumber weight, be warming up to 45 ℃-52 ℃, the band temperature defibrination that adopts the combination colloidal mill to carry out 1 hour,
(5), above-mentioned slurries are heated to 88 ℃-92 ℃, and kept temperature 5-7 minute,
(6), above-mentioned slurries are cooled to rapidly below 30 ℃, adopt gauze to filter, removing residue,
(7), adopt the inner pressed ultra-filtration equipment to separate above-mentioned filtrate, molecular weight cut-off 20,000-50,000 obtains trapped fluid and surplus liquid respectively, and surplus liquid contains amounts of protein, and surplus liquid can be made various nutritious oral liquids, also can be used as foodstuff additive,
(8), in trapped fluid, add molten long-pending 50% the dehydrated alcohol of trapped fluid, staticly settle,
(9), then move in the whizzer centrifugal, filter collection precipitation, general centrifugal 15 minutes, the precipitation part obtained centrifugate again through absolute ethanol washing, centrifugate is carried out room temperature volatilization ethanol, adopts microwave drying to get the sea cucumber polysaccharide crude product then,
(10), the sea cucumber polysaccharide crude product added in the water dissolves, be dissolved in the water of 2ml by every 100mg sea cucumber polysaccharide crude product,
(11), go up column purification, with G-150-G-200 dress post, add above-mentioned solution from capital,
(12), the water wash-out, fraction collection, through lyophilize, the pure product of sea cucumber polysaccharide.
The present invention is owing to adopted aforementioned production method, effect by means of the autolytic enzyme of fresh and alive sea cucumbers self, be aided with an amount of natural biological and make degradation product, in conjunction with the catalytic method of physics, without concentrating, do not add any poisonous and hazardous chemical solvents, changed and added the chemical degradation thing in the past, remove the chemical degradation thing again Deng after the degraded, and add acid or add the complicated technology that alkali carries out the pH value adjustment, the more complete sea cucumber polysaccharide that kept, it is simple to have production technique, production cost is low, the sea cucumber polysaccharide amount of extracting in the production process is many and quality is high, also can obtain the clout-sea cucumber liquid based on protein simultaneously, sea cucumber liquid can be made various nutritious oral liquids or foodstuff additive.
Embodiment
Below the present invention is further described:
A kind of preparation method of sea cucumber polysaccharide, adopt following steps:
(1) fresh and alive sea cucumbers is cut open the belly cleaning, fresh and alive sea cucumbers is cut open forward along belly from anus with scissors, take out intestines, clean with seawater, with saved in the later operation with salt, remove in the sea cucumber body and the silt and the foreign material on sea cucumber surface, (2) sea cucumber of cleaning is weighed, the wolfberry fruit that adds the 2%-5% of sea cucumber weight, and 2 times of water of adding sea cucumber weight, carry out fragmentation and defibrination, obtain sea cucumber milk, fragmentation can be adopted tissue mashing machine, defibrination can adopt colloidal mill, (3) add the molten 5 times long-pending water of sea cucumber milk to sea cucumber milk, heat to 40 ℃-45 ℃, and stir, constant temperature kept 2 hours, also ceaselessly stir in the thermostatic process, (4) Chinese yam of the 1%-3% of adding sea cucumber weight in above-mentioned slurries, be warming up to 45 ℃-52 ℃, the band temperature defibrination that adopts the combination colloidal mill to carry out 1 hour, (5) above-mentioned slurries are heated to 88 ℃-92 ℃, and kept temperature 5-7 minute, (6) above-mentioned slurries are cooled to rapidly below 30 ℃, adopt gauze to filter, to remove residue, obtain filtrate, (7) adopt the inner pressed ultra-filtration equipment to separate above-mentioned filtrate, molecular weight cut-off 20,000-50,000, obtain trapped fluid and surplus liquid respectively, contain amounts of protein in the surplus liquid, surplus liquid can be made various nutritious oral liquids, also can be used as foodstuff additive, (8) in trapped fluid, add molten long-pending 50% the dehydrated alcohol of trapped fluid, and staticly settle, (9) move into then in the whizzer centrifugal, filter collection precipitation, general centrifugation time is 15 minutes, the precipitation part is washed through dehydrated alcohol again, obtain centrifugate, centrifugate is carried out room temperature volatilization ethanol, adopts microwave drying to get the sea cucumber polysaccharide crude product then, and (10) add the sea cucumber polysaccharide crude product in the water and dissolve, be dissolved in the water of 2ml by every 100mg sea cucumber polysaccharide crude product, (11) go up column purification,, add above-mentioned solution from capital with G-150-G-200 dress post, (12) water wash-out, fraction collection through lyophilize, gets the pure product of sea cucumber polysaccharide.
The present invention is by means of the effect of the autolytic enzyme of fresh and alive sea cucumbers self, be aided with an amount of natural biological and make degradation product, in conjunction with the catalytic method of physics, without concentrating, do not add any poisonous and hazardous chemical solvents, changed and added the chemical degradation thing in the past, remove the chemical degradation thing again Deng after the degraded, and add acid or add the complicated technology that alkali carries out the pH value adjustment, the more complete biological activity that keeps sea cucumber polysaccharide, it is simple to have production technique, production cost is low, the sea cucumber polysaccharide amount of extracting in the production process is many and quality is high, also can obtain the clout based on protein simultaneously, surplus liquid can be made various nutritious oral liquids or foodstuff additive.
Claims (2)
1, a kind of preparation method of sea cucumber polysaccharide, feature adopt following steps:
(1), with the fresh and alive sea cucumbers cleaning of cutting open the belly,
(2), the sea cucumber of cleaning is weighed, add the wolfberry fruit of the 2%-5% of sea cucumber weight, add 2 times of water of sea cucumber weight, carry out fragmentation and defibrination, obtain sea cucumber milk,
(3), add the water of 5 times of volumes of sea cucumber milk, heat to 40 ℃-45 ℃, and stir, constant temperature kept 2 hours to sea cucumber milk,
(4), in above-mentioned slurries, add the Chinese yam of the 1%-3% of sea cucumber weight, be warming up to 45 ℃-52 ℃, the band temperature defibrination that adopts the combination colloidal mill to carry out 1 hour,
(5), above-mentioned slurries are heated to 88 ℃ → 92 ℃, and kept temperature 5-7 minute,
(6), above-mentioned slurries are cooled to rapidly below 30 ℃, filter, remove residue,
(7), adopt the inner pressed ultra-filtration equipment to separate above-mentioned filtrate, molecular weight cut-off 20,000-50,000 obtains trapped fluid and surplus liquid respectively,
(8), in trapped fluid, add molten long-pending 50% the dehydrated alcohol of trapped fluid, staticly settle,
(9), move in the whizzer centrifugal, filter collection precipitation, the ethanol that volatilizees then carries out drying again, the sea cucumber polysaccharide crude product.
2, the preparation method of a kind of sea cucumber polysaccharide according to claim 1, it is characterized in that behind the sea cucumber polysaccharide crude product, again the sea cucumber polysaccharide crude product is dissolved in by every 100mg sea cucumber polysaccharide crude product in the water of 2ml and dissolves, go up column purification then,, add above-mentioned solution from capital with G-150-G-200 dress post, last water wash-out, fraction collection through lyophilize, gets the pure product of sea cucumber polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031119255A CN1194018C (en) | 2003-03-03 | 2003-03-03 | Holothuroids polysaccharide preparing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031119255A CN1194018C (en) | 2003-03-03 | 2003-03-03 | Holothuroids polysaccharide preparing method |
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| CN1440985A CN1440985A (en) | 2003-09-10 |
| CN1194018C true CN1194018C (en) | 2005-03-23 |
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| CNB031119255A Expired - Fee Related CN1194018C (en) | 2003-03-03 | 2003-03-03 | Holothuroids polysaccharide preparing method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100336832C (en) * | 2005-10-11 | 2007-09-12 | 大连轻工业学院 | Process for extracting comb shell polysaccharide |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102030838B (en) * | 2010-10-30 | 2012-08-01 | 中国海洋大学 | High-pressure water vapor degradation method for marine sulfated polysaccharides |
| CN102488251B (en) * | 2011-12-12 | 2013-04-03 | 中国海洋大学 | Sea cucumber gelatin, preparation method thereof, medicine or health product containing sea cucumber gelatin, and application of sea cucumber gelatin |
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2003
- 2003-03-03 CN CNB031119255A patent/CN1194018C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100336832C (en) * | 2005-10-11 | 2007-09-12 | 大连轻工业学院 | Process for extracting comb shell polysaccharide |
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| CN1440985A (en) | 2003-09-10 |
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