CN102487905A - Incubation method of Cordyceps sinensis host hepialus eggs - Google Patents
Incubation method of Cordyceps sinensis host hepialus eggs Download PDFInfo
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- CN102487905A CN102487905A CN2011104311839A CN201110431183A CN102487905A CN 102487905 A CN102487905 A CN 102487905A CN 2011104311839 A CN2011104311839 A CN 2011104311839A CN 201110431183 A CN201110431183 A CN 201110431183A CN 102487905 A CN102487905 A CN 102487905A
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Abstract
The invention provides an incubation method of Cordyceps sinensis host hepialus eggs. The method comprises the steps that: in a first incubation stage from hepialus oviposition to initial egg incubation, the eggs are incubated under the condition that the eggs are maintained to be contacted with water; in a second stage from the initial egg incubation to complete egg incubation, all the eggs are transferred to the waterless condition to be incubated; the whole incubation process is carried out under the constant temperature state; at the temperature of 8-12 DEG C, the hepialus eggs are incubated under the conditions that moisture is maintained in the early days and drying is carried out in the later days, the weight and the survival rate of larva are higher than those obtained by drying incubation under the natural condition or under the moisture incubation condition all the time; and incubation is carried out at the constant temperature of 10 DEG C, the hatching rate and the survival rate are higher than those obtained under the temperature-varying incubation condition and the other constant temperature incubation conditions.
Description
Technical field
The present invention relates to the hatching m of a kind of (Lepidoptera) unicorn homopterous insect, relate to a kind of hatching m of Cordyceps sinensis host bat moth ovum in particular.
Technical background
Cordyceps sinensis Cordyceps is that aweto Cordycepssinensis (Berk.) Sacc. infects that bat belongs to the Hepialus larva and the entomogenous fungi combination that forms; The property sugariness flat; Has the effect of invigorating the lung and the kidney; Be the distinctive rare traditional Chinese medicine of China, main product is in the provinces and regions such as Sichuan, Qinghai, Yunnan, Tibet and Gansu of China.For many years, carry out the Cordyceps sinensis manual work and train the hot research problem that the research of growing is natural resources of Chinese medicinal materials protection and regeneration field always.Manual work training at Cordyceps sinensis is grown in the process; The host insect ovum during incubation will carry out certain physical or chemical treatment; Owing to carry out need ovum being exposed under the natural air humidity when these are handled, and the time that exposes is with handling different certain differences that have again.Liu Fei etc. disclose in 2006 and 2007 2 years in " natural seasoning is to the influence of Cordyceps sinensis host bat moth ovum hatching " literary composition that it is delivered, and ovum are exposed to the influence of the time length of natural seasoning to ovum hatching and newly hatched larvae survival rate.
Summary of the invention
On the research basis of the disclosed time length that ovum is exposed to natural seasoning of background technology to ovum hatching and the influence of newly hatched larvae survival rate, the present invention hatch under the condition of preserving moisture through research and temperature to the Different Effects of hatching; The hatching m that provides a kind of constant temperature to preserve moisture dry worm summer grass host bat moth ovum of later stage in winter in earlier stage is to be about to all ovum when having ovum to begin to hatch all directly transfer on the matrix for the ovum of hatching in the prior art.But the technical scheme that this patent provides is when ovum begins to hatch, all ovum to be transferred under the drying condition to hatch, and the larva of hatching is transferred on the matrix raised then.Particular content is following:
The hatching m of Cordyceps sinensis host bat moth ovum, wherein, the phase I of hatching ends when ovum begins to incubate from the bat moth egg, ovum keep with condition that water contacts under hatch; Second stage be ovum begin hatching up to all ovum all hatching finish and end, all ovum are transferred under the anhydrous condition are hatched.
The incubation temperature of said second stage is higher than 5 ℃.
But the hatching of said phase I be meant with ovum place the naked eyes water breakthrough but on the filter paper of the no water flows that tilts, relative air humidity RH 60%～70%.
Said second stage hatching is meant and ovum is placed on the dry attachment relative air humidity RH 60%～70%.
The incubation temperature of said phase I, second stage is 8～12 ℃ of constant temperature.
The condition of said hatching is:
1) ovum is produced bat moth worm's ovum with criticizing on the same day by collection, clear water washing 2～3 times;
2) but washed ovum is placed the naked eyes water breakthrough but on the filter paper of the no water flows that tilts, temperature is 10 ℃, hatch in the incubator of relative air humidity RH 60%～70%, wherein, illumination every day 12 hours, lucifuge 12 hours;
3) incubator with clear water washing ovum once, is cleaned once with thimerosal in every interval ten days between the incubation period;
When 4) ovum began to hatch larva, soon ovum was transferred on the dry filter paper by the gross, at any time the bat moth larvae that hatches is migrated out incubator, all hatched up to all ovum to finish, and whole hatching process is accomplished.
Useful technique effect of the present invention is: under 8～12 ℃ of conditions; Adopt hatching bat moth ovum under the dry condition of preserve moisture early stage in later stage; The worm of larva heavily reaches survival rate and all is higher than under the natural conditions dry hatching or is in the incubation condition of preserving moisture always; Be employed in 10 ℃ of constant temperature hatchings of temperature, incubation rate and survival rate all are higher than alternating temperature incubation condition and other constant temperature hatchings.
Embodiment
Embodiment 1 hatching humidity is investigated the influence of hatching
Collect on the same day with batch the bat moth worm's ovum that produces as the terminal drying group; Clear water washing 2～3 times; But washed ovum is placed the naked eyes water breakthrough but on the filter paper of the no water flows that tilts, temperature is 8～12 ℃, hatch in the incubator of relative air humidity RH 60%～70%, wherein, illumination every day 12 hours, lucifuge 12 hours; Every interval is ten days between the incubation period, with clear water washing ovum once, cleans incubator once with thimerosal; When ovum begins to hatch larva, be about to ovum by the gross and all transfer in the anhydrous incubator that is equipped with dry filter paper, at any time the bat moth larvae that hatches is selected incubator, render on the matrix, every box is thrown in 30 larvas, and whole hatching process is accomplished; Each handles the survival rate of larva statistics larva after raising about 2 months under the identical condition of hatching with each stage.
Collect on the same day with batch institute's bat moth worm's ovum that produces as preserving moisture the hatching group, but the overall process of hatching places the naked eyes water breakthrough still on the filter paper of the no water flows of inclination on washed ovum, cultivation temperature is 8～12 ℃; Hatch in the incubator of relative air humidity RH 60%～70%, wherein, illumination every day 12 hours, lucifuge 12 hours; Every interval is ten days between the incubation period, with clear water washing ovum once, cleans incubator once with thimerosal; At any time the bat moth larvae that hatches is selected incubator, render on the matrix, every box is thrown in 30 larvas, and whole hatching process is accomplished; Each handles the survival rate of larva statistics larva after raising about 2 months under the identical condition of hatching with each stage.The relatively terminal drying hatching group and the influence (every box throw in 30 larvas) of hatching group of preserving moisture all the time to the growth of larva subsequent growth.The 3rd time timing statistics is the mature larva stage.Concrete outcome is as shown in table 1.
Table 1 hatching humidity is investigated the influence of hatching
Can find out that from table 1 body weight and the survival rate of terminal drying group larva all are significantly higher than the hatching group of preserving moisture when adding up for the first time.To the 2nd when statistics,, finally cause the survival rate and the hatching group of preserving moisture to handle no significant difference, but the body weight of larva still is higher than dry hatching group processing because the larva that becomes to live is too many or because larva is energetic, cause cutting one another's throats between the terminal drying group larva.During to the 3rd statistics, each handles continued growth on original basis, because the difference of handling, thereby make that terminal drying group larva is all to be higher than the hatching group of preserving moisture to handle in body weight or on survival rate.So can find out from The above results, ovum adopts the terminal drying group that the survival rate at larva initial stage not only can significantly be provided, and growth also has significant facilitation to the larva growth of later stage.
Embodiment 2 incubation temperatures are investigated the influence of hatching
Adopt the hatching m of embodiment 1 described terminal drying group, the ovum that 3 pairs of mating bats of mixed collection moth produces.According to the time of ovum conventional incubation, its average mark is done A, B, three time periods of C, the A time in stage is that 15 days, B stage are 15 days, C stage from the B stage finishes to finish to all egg hatches; The alternating temperature group is chosen 5 ℃, 10 ℃ and 15 ℃ and is the research temperature; The constant temperature group is chosen 8 ℃, 10 ℃, 12 ℃, 14 ℃ and 16 ℃ and is research object, each group process A, B and three time period of C; After the constant temperature processing of A time period finishes, transfer to the constant temperature of B time period rapidly and handle, and then transfer to the constant temperature processing of C time period rapidly and last till that always ovum begins hatching.Handle repetition 3 times for every group, whenever repeat to throw in 50 ovum, the larva that is hatched is raised respectively by the conventional feeding manner of industrialization, and each is raised unit and throws in 30 larvas, establishes 3 repetitions.The degree that hatching culture dish filter paper is preserved moisture is a water breakthrough and not flowing, and hatching natural air humidity RH is 60%～70%.Survival rate after the statistics ovum is gone through the phase in the average hatching under the different disposal condition, incubation rate and the larva that incubates survive the winter.
Table 2 incubation temperature is to the influence of hatching
Can know that from table 2 alternating temperature group ovum compares the phase of going through that more helps shortening ovum at the low temperature of 5 ℃ of middle and later periods experience at the low temperature that in earlier stage experiences 5 ℃, the phase of going through under indoor natural temperature condition was the shortest, the phase of going through of all the other processing all was higher than the experimental group VIII of 10 ℃ of constant temperature; Constant temperature culture group and shortening along with the rising of temperature lasts.This result shows that the low temperature that experiences 5 ℃ possibly be to cause to last the immediate cause that is higher than the experimental group VIII.When other processing has all been hatched; Group I and experimental group III are not but seen hatching 5 ℃ latter stage under the temperature condition all the time; Compare with the experimental group VIII postpone about 1 week after, the researcher transfers to it under about 10 ℃ natural temperature group XII condition from 5 ℃ of insulating boxs, just begins hatching in second day.This result shows, will go through the phase by significant prolongation if run into low temperature in the latter stage of ovum hatching, and its reason possibly be that 5 ℃ low temperature is unfavorable for that the fully-developed larva pips.
The incubation rate of the experimental group VIII of constant temperature group is up to 77.34%, and the incubation rate of experimental group X is minimum to be merely 70.70%; Alternating temperature group incubation rate is the highest to be that the experimental group II is 71.67%, and the incubation rate of experimental group III is minimum to be merely 65.00%.Incubation rate there was no significant difference between statistical results show group I and the experimental group III (p>0.05); There was no significant difference between the incubation rate of experimental group II, IV, V, VI, VII and experimental group VIII, but be significantly higher than the incubation rate of group I and III.This result shows, as long as the C time period of ovum hatching all is unfavorable for the raising of egg hatching rate when being 5 ℃, and in A time period and B time period, being in 5 ℃, 10 ℃ or 15 ℃ does not all have appreciable impact to the incubation rate of ovum.In the constant temperature group, 10 ℃ is the optimum condition of hatching, along with temperature rising incubation rate is on a declining curve.
Survival rate is up to 66.00% of experimental group VIII in the constant temperature experimental group, and survival rate is minimum to be 62.00% of experimental group X; To be up to the experimental group V be 64.00% to survival rate in the alternating temperature experimental group, and the survival rate of group I is minimum to be merely 23.00%.Statistical results show, there was no significant difference between experimental group VIII and the experimental group V (p>0.05), but be significantly higher than the survival rate (0.01 < p < 0.05) of group I.This result shows that the larva of constant temperature hatching even do not pass through domestication by low temperature, can adapt to the low temperature of larva overwintering period equally.But the ovum hatching will be unfavorable for the one-tenth work of newly hatched larvae latter stage like 5 ℃ low temperature.In the constant temperature group, 10 ℃ is the optimum condition of hatching, along with temperature rising survival rate is on a declining curve.
Claims (6)
1. the hatching m of Cordyceps sinensis host bat moth ovum is characterized in that: the phase I of hatching ends when ovum begins to incubate from the bat moth egg, ovum keep with condition that water contacts under hatch; Second stage be ovum begin hatching up to all ovum all hatching finish and end, all ovum are transferred under the anhydrous condition are hatched.
2. the hatching m of Cordyceps sinensis host bat moth ovum according to claim 1, it is characterized in that: the incubation temperature of said second stage is higher than 5 ℃.
3. the hatching m of Cordyceps sinensis host bat moth ovum according to claim 1 is characterized in that: but said phase I hatching be meant with ovum place the naked eyes water breakthrough but on the filter paper of the no water flows that tilts, relative air humidity RH 60%～70%.
4. the hatching m of Cordyceps sinensis host bat moth ovum according to claim 1 is characterized in that: said second stage hatching is meant and ovum is placed on the dry attachment relative air humidity RH 60%～70%.
5. the hatching m of Cordyceps sinensis host bat moth ovum according to claim 1 is characterized in that: the incubation temperature of said phase I, second stage is 8～12 ℃ of constant temperature.
6. according to the hatching m of claim 1,2,3,4 or 5 described Cordyceps sinensis host bat moth ovum, it is characterized in that: the condition of said hatching is:
1) ovum is produced bat moth worm's ovum with criticizing on the same day by collection, clear water washing 2～3 times;
2) but washed ovum is placed the naked eyes water breakthrough but on the filter paper of the no water flows that tilts, temperature is 10 ℃, hatch in the incubator of relative air humidity RH 60%～70%, wherein, illumination every day 12 hours, lucifuge 12 hours;
3) incubator with clear water washing ovum once, is cleaned once with thimerosal in every interval ten days between the incubation period;
When 4) ovum began to hatch larva, soon ovum was transferred on the dry filter paper by the gross, at any time the bat moth larvae that hatches is migrated out incubator, all hatched up to all ovum to finish, and whole hatching process is accomplished.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102835357A (en) * | 2012-09-24 | 2012-12-26 | 中山大学 | Method for improving mating rate of thitarodes adults |
| CN102835358A (en) * | 2012-09-24 | 2012-12-26 | 中山大学 | Breeding method of thitarodes armoricanus larvae |
| CN103081869A (en) * | 2012-08-16 | 2013-05-08 | 重庆市中药研究院 | Method for artificially cultivating cordyceps sinensis at low altitude |
| CN103918480A (en) * | 2014-04-11 | 2014-07-16 | 钱志强 | Artificial culturing method for cordyceps sinensis |
| CN104322451A (en) * | 2014-08-20 | 2015-02-04 | 广东省昆虫研究所 | Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis |
| CN106614400A (en) * | 2016-12-27 | 2017-05-10 | 香格里拉市宜康宝生物科技有限公司 | Prediction method for bat moth hatching rate |
| CN110384077A (en) * | 2019-08-30 | 2019-10-29 | 贵州省烟草公司六盘水市公司 | A kind of Candle-sticks stinkbug egg hatching method |
| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081869A (en) * | 2012-08-16 | 2013-05-08 | 重庆市中药研究院 | Method for artificially cultivating cordyceps sinensis at low altitude |
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| CN103918480A (en) * | 2014-04-11 | 2014-07-16 | 钱志强 | Artificial culturing method for cordyceps sinensis |
| CN104322451A (en) * | 2014-08-20 | 2015-02-04 | 广东省昆虫研究所 | Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis |
| WO2016026237A1 (en) * | 2014-08-20 | 2016-02-25 | 广东省昆虫研究所 | Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis |
| US10412941B2 (en) | 2014-08-20 | 2019-09-17 | Guangdong Institute Of Applied Biological Resources | Artificial feeding method at low altitude for host insect ghost moth of ophiocordyceps sinensis |
| CN106614400A (en) * | 2016-12-27 | 2017-05-10 | 香格里拉市宜康宝生物科技有限公司 | Prediction method for bat moth hatching rate |
| CN110384077A (en) * | 2019-08-30 | 2019-10-29 | 贵州省烟草公司六盘水市公司 | A kind of Candle-sticks stinkbug egg hatching method |
| CN110384077B (en) * | 2019-08-30 | 2021-11-16 | 贵州省烟草公司六盘水市公司 | Lugworm egg hatching method |
| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
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Application publication date: 20120613 |