CN102482385B - 用来培养细胞的合成微型载体 - Google Patents

用来培养细胞的合成微型载体 Download PDF

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Publication number
CN102482385B
CN102482385B CN201080033364.XA CN201080033364A CN102482385B CN 102482385 B CN102482385 B CN 102482385B CN 201080033364 A CN201080033364 A CN 201080033364A CN 102482385 B CN102482385 B CN 102482385B
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China
Prior art keywords
microcarriers
cells
unsaturated monomer
hydrophilic
microcarrier
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Expired - Fee Related
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CN201080033364.XA
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English (en)
Chinese (zh)
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CN102482385A (zh
Inventor
S·德赛
D·亨利
M·赫维
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Corning Inc
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Corning Inc
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F289/00—Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds not provided for in groups C08F251/00 - C08F287/00
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2/00—Processes of polymerisation
    • C08F2/32—Polymerisation in water-in-oil emulsions
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/26—Esters containing oxygen in addition to the carboxy oxygen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
    • C08F222/36—Amides or imides
    • C08F222/38—Amides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201080033364.XA 2009-05-28 2010-05-27 用来培养细胞的合成微型载体 Expired - Fee Related CN102482385B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18177609P 2009-05-28 2009-05-28
US61/181,776 2009-05-28
PCT/US2010/036351 WO2010138687A1 (en) 2009-05-28 2010-05-27 Synthetic microcarriers for culturing cells

Publications (2)

Publication Number Publication Date
CN102482385A CN102482385A (zh) 2012-05-30
CN102482385B true CN102482385B (zh) 2014-10-15

Family

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CN201080033364.XA Expired - Fee Related CN102482385B (zh) 2009-05-28 2010-05-27 用来培养细胞的合成微型载体

Country Status (6)

Country Link
US (2) US9115238B2 (cg-RX-API-DMAC7.html)
EP (2) EP2435490A1 (cg-RX-API-DMAC7.html)
JP (2) JP5818785B2 (cg-RX-API-DMAC7.html)
CN (1) CN102482385B (cg-RX-API-DMAC7.html)
TW (1) TW201042038A (cg-RX-API-DMAC7.html)
WO (2) WO2010138702A1 (cg-RX-API-DMAC7.html)

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CN101939418A (zh) 2008-01-30 2011-01-05 杰龙公司 用于培养干细胞衍生的心肌细胞的合成表面
CN101939362A (zh) 2008-01-30 2011-01-05 杰龙公司 用于培养干细胞衍生的少突胶质细胞前体细胞的合成表面
WO2009099552A2 (en) * 2008-01-30 2009-08-13 Corning Incorporated Cell culture article and screening
WO2014209865A1 (en) * 2013-06-24 2014-12-31 Corning Incorporated Cell culture article and methods thereof
US9725689B2 (en) 2010-10-08 2017-08-08 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US9534206B2 (en) 2010-12-16 2017-01-03 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9926523B2 (en) 2010-12-16 2018-03-27 General Electric Company Cell carriers and methods for culturing cells
US9453196B2 (en) 2010-12-16 2016-09-27 General Electric Company Cell carrier, methods of making and use
US9453197B2 (en) 2010-12-16 2016-09-27 General Electric Company Methods of making cell carrier
US9518249B2 (en) 2010-12-16 2016-12-13 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
AU2012256403B2 (en) 2011-03-17 2016-10-20 Corning Incorporated Synthetic coating for cell culture
US9376655B2 (en) 2011-09-29 2016-06-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
KR101886432B1 (ko) * 2011-12-23 2018-09-07 엘지디스플레이 주식회사 유기전계발광표시장치 및 이의 제조 방법
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CN104395457B (zh) 2012-02-02 2018-02-27 康宁股份有限公司 用于细胞培养的合成粘附培养基
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JP6299084B2 (ja) * 2013-05-31 2018-03-28 大日本印刷株式会社 細胞培養方法及びそれに用いるマイクロキャリア
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EP3068867B1 (en) 2013-11-16 2018-04-18 Terumo BCT, Inc. Expanding cells in a bioreactor
US11008547B2 (en) 2014-03-25 2021-05-18 Terumo Bct, Inc. Passive replacement of media
CN103980442B (zh) * 2014-05-29 2016-05-18 西南石油大学 用于稠油乳化降粘的水溶性聚合物及其制备方法
JP6104312B2 (ja) * 2014-06-19 2017-03-29 日東電工株式会社 組織再生促進剤
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US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
CN110177870B (zh) * 2017-01-20 2023-10-27 新加坡科技研究局 热响应性微载体系统及其用途
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
JP7393945B2 (ja) 2017-03-31 2023-12-07 テルモ ビーシーティー、インコーポレーテッド 細胞増殖
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
EP3830237A1 (en) * 2018-08-16 2021-06-09 TERUMO Kabushiki Kaisha Cell culture substrate
JP7378212B2 (ja) * 2019-02-28 2023-11-13 浜松ホトニクス株式会社 細胞接着用組成物及び細胞接着用基材
CN113966391A (zh) 2019-06-13 2022-01-21 康宁股份有限公司 用于培养细胞的可溶性微载体及相关方法
US12692475B2 (en) 2020-09-15 2026-07-28 Lg Chem, Ltd. Microcarrier for cell culture and method for preparing the same
CN116783280A (zh) * 2021-02-03 2023-09-19 积水化学工业株式会社 细胞培养用微载体和细胞的培养方法
WO2022168870A1 (ja) * 2021-02-03 2022-08-11 積水化学工業株式会社 細胞培養用マイクロキャリア及び細胞の培養方法
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Also Published As

Publication number Publication date
JP2012527901A (ja) 2012-11-12
JP5818785B2 (ja) 2015-11-18
US9115238B2 (en) 2015-08-25
EP2435490A1 (en) 2012-04-04
WO2010138687A1 (en) 2010-12-02
US20100304482A1 (en) 2010-12-02
JP5792714B2 (ja) 2015-10-14
TW201042038A (en) 2010-12-01
US8426176B2 (en) 2013-04-23
CN102482385A (zh) 2012-05-30
WO2010138702A1 (en) 2010-12-02
US20100317113A1 (en) 2010-12-16
EP2435489A1 (en) 2012-04-04
JP2012527902A (ja) 2012-11-12

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Granted publication date: 20141015

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CF01 Termination of patent right due to non-payment of annual fee