CN102466877A - Confocal microscope system of computer - Google Patents
Confocal microscope system of computer Download PDFInfo
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- CN102466877A CN102466877A CN 201010541230 CN201010541230A CN102466877A CN 102466877 A CN102466877 A CN 102466877A CN 201010541230 CN201010541230 CN 201010541230 CN 201010541230 A CN201010541230 A CN 201010541230A CN 102466877 A CN102466877 A CN 102466877A
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Abstract
The invention relates to a confocal microscope system of a computer. A control system of the computer comprises: a variable arrangement and display device, which is used for arranging a polling variable. a polling controller, which is used for controlling all polling devices of a confocal microscope to carry out movements according to a polling variable arranged by a stereo variable arrangement device so as to realize corresponed polling of X and Y planes; a positioner, which is used for controlling a power demultiplier, a spatial filter, and a microbit device of the confocal microscope to carry out precision localization according to the polling variable arranged by the stereo variable arrangement device; and a channel selector, which is used for carrying out selection and conversion on a signal monitoring and detecting optical path of the confocal microscope according to a polling variable by the variable arrangement and display device. According to the invention, automatic control of the system can be realized and it is convenient for a user to carry out operation and usage. Moreover, polling imaging of a living sample can be realized; and processing and an analysis on a fluorescent fault graph as well as visualization of a stereo data field can also be realized.
Description
Technical field
The invention belongs to the microscopy field, particularly a kind of computing machine confocal microscopy mirror system.
Background technology
Confocal microscope is a kind of optical microphotograph imaging system, can carry out the imaging of optical fault poll, and the sequence tomography figure of its poll can carry out solid figure reconstruct, thereby understands inner structure, the external morphology of samples such as biological tissue and material.
Common confocal microscope mostly can not be realized the confocal poll micro-imaging of not lacking of proper care of living specimen at present; The present invention has realized the computer-controlled confocal poll imaging of automatically not lacking of proper care, and has realized processing, analysis and stereo data field visual of fluorescence tomography poll figure.
Summary of the invention
The technical matters that the present invention will solve is exactly the problem of prior art function singleness, for people provide a kind of reconstruct that collects collection, processing, analysis and the stereo data field of poll, light figure, are shown as the Computerized laser confocal poll microscopic system of one.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
The present invention is made up of confocal microscope and computer control system two large divisions, it is characterized in that, said computer control system comprises:
1, the variable that is used to be provided with the poll variable is established apparent device;
2, according to three-dimensional variable the poll variable that device is provided with is set, each poll device of control confocal microscope moves, and realizes corresponding X, the poll controller of Y plane poll;
3, according to three-dimensional variable the poll variable that device is provided with is set, power decay device, spatial filter and the microbit device of control confocal microscope carry out pinpoint steady arm;
4, according to establishing apparent poll variable, the channel to channel adapter that the signal detection light path of confocal microscope is selected, changed;
5, the graphic process unit of the poll figure of being gathered being handled and being calculated;
6, the sequence tomography figure of being gathered is carried out the stereo reconstruction device of stereo reconstruction and demonstration.
The robotization control of feasible system of the present invention is convenient to the user and is manipulated.Not only can realize the imaging of the poll of living specimen, and can realize processing, analysis and stereo data field visual of fluorescence tomography figure.
Description of drawings
Accompanying drawing is a connection synoptic diagram of the present invention.
Embodiment
Shown in accompanying drawing, the present invention is made up of confocal microscope and control system two large divisions.Wherein, confocal microscope is by laser instrument, the power decay device; X, Y direction poll galvanometer, the danger signal that Z constitutes to the microbit device, by spatial filter people, photodetector people, amplifier A is surveyed light path; By spatial filter B; Photodetector B, the green that amplifier B constitutes is surveyed light path, formations such as A/D converter.The variable that the present invention's three-dimensional variable is provided with the device setting comprises totally eight variablees such as feature size, pel spacing, poll scope, laser power, spatial filter, channel selecting, poll speed, wave filter.Below explain as follows one by one:
1, feature size
Feature size has 1024*1024,512*512,256*256, four kinds of 128*128 optional.According to the size of selected figure, control system control X, the sampled point number of Y direction poll galvanometer, thus change sample by the scope of poll.
2, pel spacing
Pel spacing have 14 kinds optional.According to selected pel spacing size, control system control X, the sampled point of Y direction poll galvanometer is asked distance, thereby has changed subjective magnification of figure and resolution.
3, poll scope
The setting of poll scope is meant under the certain situation of feature size and pel spacing, according to selected poll position, and computer control X, the start position of l ' direction poll galvanometer, thus change the center of poll.Consider the aperture problem of object lens, X and h direction respectively have 3 grades optional.
4, laser power
Laser power have 8 grades optional. because the laser power that excites is when very strong, radiofluorescence is very strong, has influenced graphics details, therefore utilizes control system power controlling demultiplier that laser power is decayed.
5. spatial filter
The size of spatial filter diameter directly influences the resolution of system. and confocal microscope adopts 5 kinds of wave filters not of uniform size. and control system is sent control signal and to steady arm corresponding spatial filter is carried out the precision location.
6, channel selecting
Channel selecting has 3 kinds of modes. and be red channel, green channel, 3 kinds of working methods of red channel and green channel.
Computing machine sends control signal and to channel to channel adapter passage is selected.
7, poll speed
Poll speed has 4 kinds of modes optional, promptly supper-fast, and is normal fast, at a slow speed. through the control rate selector switch, change the poll speed of confocal microscope
8, wave filter
Receive the several factors influence owing to gather graphical quality, so figure usually has noise, the present invention is provided with various filters. and power decay device, spatial filter and Z that the present invention's steady arm is used to control confocal microscope accurately locate to the microbit device.
In the present embodiment; The laser power of confocal microscope has 8 grades. because the laser power that excites is when strong; Influence the graphics details that poll is gathered easily, therefore need to utilize the power decay device that laser power is decayed, because the size of spatial filter directly influences the resolution of system according to demands of different. therefore; In the present embodiment, confocal microscope has adopted 5 kinds of spatial filters not of uniform size.Before the work of poll galvanometer; Steady arm is provided with the variable that device is provided with according to the three-dimensional variable of poll; Send control signal, require to drive the light-balancing filter and the space filter of power decay device according to control corresponding, thereby both are accurately located through corresponding stepper motor.The present invention's channel to channel adapter is used for the signal detection light path of confocal microscope is selected, changed.
In the present embodiment, the acquisition of signal light path of confocal laser poll appearance has two kinds of red channel and green channels, and working method has 3 kinds of red channel, green channel, red channel and green channels.At steady arm power decay device and spatial filter are carried out the pinpoint while; Channel to channel adapter is provided with the variable that device is provided with according to three-dimensional variable; Passage is carried out, and the present invention's graphic process unit is used for the figure of preserving or gather is in real time handled and calculated.Several work below it is mainly accomplished: figure filtering; The adjustment of figure shading value; Pantography; Pseudo-colours is handled; Calculate; Fluorescence labeling.The present invention's stereo reconstruction device is used for figure is carried out stereo reconstruction and demonstration, several work below it is mainly accomplished: open the tomography figure; The data fields projective transformation; Three-dimensional volume data is converted into 2-D data.
Claims (2)
1. computing machine confocal microscopy mirror system is characterized in that the control system of said computing machine comprises: the variable that is used to be provided with the poll variable is established apparent device; According to three-dimensional variable the poll variable that device is provided with is set, each poll device of control confocal microscope moves, and realizes corresponding X, the poll controller of Y plane poll; According to three-dimensional variable the poll variable that device is provided with is set, power decay device, spatial filter and the microbit device of control confocal microscope carry out pinpoint steady arm; According to establishing apparent poll variable, the channel to channel adapter that the signal detection light path of confocal microscope is selected, changed; The graphic process unit that the poll figure of being gathered is handled and calculated; The sequence tomography figure of being gathered is carried out the stereo reconstruction device of stereo reconstruction and demonstration.
2. a kind of computing machine confocal microscopy mirror system according to claim 1; It is characterized in that the variable that three-dimensional variable is provided with the device setting comprises feature size, pel spacing, poll scope, laser power, spatial filter, channel selecting, poll speed, wave filter totally eight variablees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010541230 CN102466877A (en) | 2010-11-09 | 2010-11-09 | Confocal microscope system of computer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010541230 CN102466877A (en) | 2010-11-09 | 2010-11-09 | Confocal microscope system of computer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102466877A true CN102466877A (en) | 2012-05-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010541230 Pending CN102466877A (en) | 2010-11-09 | 2010-11-09 | Confocal microscope system of computer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102466877A (en) |
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2010
- 2010-11-09 CN CN 201010541230 patent/CN102466877A/en active Pending
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Application publication date: 20120523 |