CN103487419A - Confocal laser scanning micro-imaging system - Google Patents
Confocal laser scanning micro-imaging system Download PDFInfo
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- CN103487419A CN103487419A CN201310431090.5A CN201310431090A CN103487419A CN 103487419 A CN103487419 A CN 103487419A CN 201310431090 A CN201310431090 A CN 201310431090A CN 103487419 A CN103487419 A CN 103487419A
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Abstract
The invention relates to a confocal laser scanning micro-imaging system. The hardware of the confocal laser scanning micro-imaging system comprises a laser light source, an optical microimaging part, a system scanning and controlling part as well as a computer image processing, image analysis and visualization part; the software of the confocal laser scanning micro-imaging system comprises a systems control module, a fluorescence image acquisition module, a fluorescence sectional image processing and analyzing module and a three-dimensional data field visualization module. The confocal laser scanning micro-imaging system provided by the invention has the advantages of high precision, high scanning speed and good stability performance, and is a high-precision detection and analysis system which integrates optics, machinery, electronics and a computer into a whole, and can meet the requirement of micro-detection of biomedicine, and has extremely high plane resolution and depth resolution.
Description
Technical field
The present invention relates to micro-imaging technique, relate in particular to a kind of laser confocal scanning micro imaging system.
Background technology
Along with scientific and technical development, microscopical purposes and type are also constantly being upgraded and are expanding, microscope commonly used can be divided into a series of scanning probe microscopies of ordinary optical microscope, fluorescent microscope, transmission electron microscope, field emission microscope, scanning electron microscope etc. and the eighties new development etc. usually, although type and purposes are different, all micro-imaging techniques are all to carry out amplification imaging by sample and the dissimilar ray obtaining information that interacts differently.
At present, along with the development of laser technology, modern microelectric technique, computer technology and image processing techniques, make modern Biomedical Instruments enter to take the stage that computing machine is main body.High performance computing machine and abundant software, make advanced medical instrument and equipment have more powerful function and perfect performance, for development and the clinical medicine of biomedical engineering provides strong instrument.
But, in order to meet the application and research demand on actual biomedicine, the laser confocal scanning microscope that needs the higher flat resolution of exploitation and depth resolution, and need better image processing and image analysis technology to be processed and analyze the fluoroscopic image gathered, utilize computer graphical to gain knowledge the three-dimensional sample variance data fields of biosome is carried out to three-dimensionalreconstruction, generate the three-dimensional appearance of object, these need precision higher, faster, the better laser confocal scanning micro imaging system of stability of sweep velocity.
Summary of the invention
The present invention has overcome the deficiencies in the prior art, and the laser confocal scanning that a kind of precision is high, sweep velocity is fast, stability is good micro imaging system is provided.
For achieving the above object, the technical solution used in the present invention is: a kind of laser confocal scanning micro imaging system, its system hardware comprises laser light source part, optical microphotograph imaging moiety, system scan control section and Computer Image Processing, graphical analysis and visual part; Its software comprises following a few part: the visualization model of system control module, fluoroscopic image acquisition module, the processing of fluorescence faultage image and analysis module and 3 d data field, described system control module is sent the scan control signal of directions X and Y-direction by computing machine, carry out digital-to-analog conversion by D/A, simulating signal is inputted scanning device separately, realizes the two-way flat scanning of X, Y of laser; Described fluoroscopic image acquisition module is that sample sends fluorescence under the exciting of scan laser point, focus on spatial filter by confocal optical system, by photodetector and amplifier input a/d converter, computing machine is by the collection that synchronously realizes fluoroscopic image of gated sweep device and A/D converter again; Described fluorescence faultage image process and analysis module for the treatment of the image collected with the described fluoroscopic image acquisition module of analysis; Described 3 d data field visualization model is for obtaining the three-dimensional body visual image of high flat resolution and high depth resolution.
In a preferred embodiment of the present invention, further comprise, described fluorescence faultage image processing and analysis module carry out the Treatment Analysis flow process to image and comprise image processing, image calculation and image fluorescence labeling.
In a preferred embodiment of the present invention, further comprise, described image process comprise following one or more: image filtering, the adjustment of image shading value, image scaling, pseudo-colours are processed and the figure image intensifying.
In a preferred embodiment of the present invention, further comprise, described image calculation comprises that the straight line for gray count on straight line calculates and calculates for the zone of the gray count on zone.
In a preferred embodiment of the present invention, further comprise, described image fluorescence labeling comprises to piece image is added the image list mark of color and the two width images of opening added respectively to the two marks of image of different colours.
In a preferred embodiment of the present invention, further comprise, described 3 d data field visualization model is for field visualized three-dimensionalreconstruction and arbitrary section reconstruct two aspects of comprising of system three-dimensional data.
In a preferred embodiment of the present invention, further comprise, described fluoroscopic image acquisition module is that the system acquisition image comprises image acquisition parameter setting and image acquisition two parts.
In a preferred embodiment of the present invention, further comprise, described image acquisition parameter setting comprises following one or more: the setting of image size, image picture pitch setting, sweep limit setting, laser power setting, spatial filter setting, scan channel setting, sweep velocity setting and noise filter setting.
In a preferred embodiment of the present invention, further comprise, described image acquisition comprise following one or more: image prescan, image Single Slice Mode, image Multi Slice Mode and image real time scan.
The invention solves the defect existed in background technology, there is following beneficial effect:
The invention provides the laser confocal scanning micro imaging system that a kind of precision is high, sweep velocity is fast, stability is good, it is light harvesting, machinery, electronics, integrated high-precision detection and the analytic system of computing machine, this system can meet the needs of biomedical microcosmic detection, and it not only has very high flat resolution but also has very high depth resolution.
The accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the module map of the preferred embodiments of the present invention.
Embodiment
The present invention is further detailed explanation in conjunction with the accompanying drawings and embodiments now, and these accompanying drawings are the schematic diagram of simplification, basic structure of the present invention only is described in a schematic way, so it only shows the formation relevant with the present invention.
As shown in Figure 1, the invention provides a kind of laser confocal scanning micro imaging system, its system hardware comprises laser light source part, optical microphotograph imaging moiety, system scan control section and Computer Image Processing, graphical analysis and visual part; Its software comprises following a few part: the visualization model of system control module, fluoroscopic image acquisition module, the processing of fluorescence faultage image and analysis module and 3 d data field.
Wherein, system control module is sent the scan control signal of directions X and Y-direction by computing machine, by D/A, carry out digital-to-analog conversion, and simulating signal is inputted scanning device separately, realizes the two-way flat scanning of X, Y of laser; Simultaneously by controlling counting of X, Y-direction analyzing spot, the spacing of analyzing spot, the position of sweep starting point, realize that sample is scanned position and the scope that is scanned is selected.According to without the scan mode requirement, computing machine sends corresponding control signal, by serial port, inputs Z-direction micrometric displacement device controller, realizes that accurately location and Z-direction equidistantly move Z-direction.Computing machine sends control signal input steady arm, controlling spatial filter accurately locates, the fluoroscopic image acquisition module is that sample sends fluorescence under the exciting of scan laser point, focus on spatial filter by confocal optical system, by photodetector and amplifier input a/d converter, computing machine is by the collection that synchronously realizes fluoroscopic image of gated sweep device and A/D converter again.
Image capture module is that the system acquisition image comprises image acquisition parameter setting and image acquisition two parts.Wherein, the image acquisition parameter setting comprises the setting of image size, image picture pitch setting, sweep limit setting, laser power setting, spatial filter setting, scan channel setting, sweep velocity setting and noise filter setting.Image acquisition comprises image prescan, image Single Slice Mode, image Multi Slice Mode and image real time scan.
The fluorescence faultage image process and analysis module for the treatment of the image collected with the described fluoroscopic image acquisition module of analysis; The processing of fluorescence faultage image and analysis module carry out the Treatment Analysis flow process to image and comprise image processing, image calculation and image fluorescence labeling; Wherein, image is processed and is comprised image filtering, the adjustment of image shading value, image scaling, pseudo-colours processing and figure image intensifying.Image calculation comprises that the straight line for gray count on straight line calculates and calculates for the zone of the gray count on zone.The image fluorescence labeling comprises to piece image is added the image list mark of color and the two width images of opening added respectively to the two marks of image of different colours.
The 3 d data field visualization model is for obtaining the three-dimensional body visual image of high flat resolution and high depth resolution.The 3 d data field visualization model is for field visualized three-dimensionalreconstruction and arbitrary section reconstruct two aspects of comprising of system three-dimensional data.
Traditional iso-surface patch method reconstruct three-dimensional shaped is known from experience the abundant details of loss image, in laser confocal scanning microscope system of the present invention, has used object plotting method, and implementation step is as follows:
(1) open the sequence faultage image, read the data value of faultage image, according to the faultage image acquisition order and laterally, sweep span longitudinally, structure 3 d-dem data fields;
(2) determine 3 d-dem data fields position and size in view coordinate;
(3) 3 d-dem data fields in view coordinate is carried out to projective transformation;
(4) resampling, carry out image and synthesize, and the composograph on view plane is carried out to the urban district conversion, by the screen of image demonstration and display;
(5) if need to the 3-D view of reconstruct be rotated, input the angle of sense of rotation, then carry out three-dimensional graphic reconstruction.
Because the laser confocal scanning microscope system can only scan the image on XY, XZ, YZ plane, and can not scan the arbitrary section image, therefore need to carry out arbitrary section reconstruct to the 3 d-dem data fields.Because laser confocal microscope has very high plane and depth resolution, so 3 d-dem data fields arbitrary section can both reconstruct, the method that its reconstruct realizes is as follows:
(1) open serial faultage image, read the faultage image data value, according to the faultage image acquisition order and laterally, sweep span longitudinally, structure 3 d-dem data fields, given arbitrary section position dough-kneading method vector;
(2) the 3 d-dem data fields is carried out to resampling;
(3) carry out interpolation calculation, image is synthetic;
(4) carry out positive parallel projection and vision area conversion, show cross-sectional image; If image will rotate, input the normal vector of the surfaces of revolution, re-start reconstruct.
Laser confocal scanning micro imaging system of the present invention is light harvesting, machinery, electronics, integrated high-precision detection and the analytic system of computing machine, this system can meet the needs of biomedical microcosmic detection, and it not only has very high flat resolution but also has very high depth resolution.
Above foundation desirable embodiment of the present invention is enlightenment, and by above-mentioned description, the related personnel can, in the scope that does not depart from this invention technological thought, carry out various change and modification fully.The technical scope of this invention is not limited to the content on instructions, must determine technical scope according to the claim scope.
Claims (9)
1. a laser confocal scanning micro imaging system, is characterized in that, its system hardware comprises laser light source part, optical microphotograph imaging moiety, system scan control section and Computer Image Processing, graphical analysis and visual part; Its software comprises following a few part: the visualization model of system control module, fluoroscopic image acquisition module, the processing of fluorescence faultage image and analysis module and 3 d data field, described system control module is sent the scan control signal of directions X and Y-direction by computing machine, carry out digital-to-analog conversion by D/A, simulating signal is inputted scanning device separately, realizes the two-way flat scanning of X, Y of laser; Described fluoroscopic image acquisition module is that sample sends fluorescence under the exciting of scan laser point, focus on spatial filter by confocal optical system, by photodetector and amplifier input a/d converter, computing machine is by the collection that synchronously realizes fluoroscopic image of gated sweep device and A/D converter again; Described fluorescence faultage image process and analysis module for the treatment of the image collected with the described fluoroscopic image acquisition module of analysis; Described 3 d data field visualization model is for obtaining the three-dimensional body visual image of high flat resolution and high depth resolution.
2. laser confocal scanning micro imaging system according to claim 1, is characterized in that, described fluorescence faultage image processing and analysis module carry out the Treatment Analysis flow process to image and comprise image processing, image calculation and image fluorescence labeling.
3. laser confocal scanning micro imaging system according to claim 2, is characterized in that, described image process comprise following one or more: image filtering, the adjustment of image shading value, image scaling, pseudo-colours are processed and the figure image intensifying.
4. laser confocal scanning micro imaging system according to claim 2, is characterized in that, described image calculation comprises that the straight line for gray count on straight line calculates and calculates for the zone of the gray count on zone.
5. laser confocal scanning micro imaging system according to claim 2, is characterized in that, described image fluorescence labeling comprises to piece image is added the image list mark of color and the two width images of opening added respectively to the two marks of image of different colours.
6. laser confocal scanning micro imaging system according to claim 1, is characterized in that, described 3 d data field visualization model is for field visualized three-dimensionalreconstruction and arbitrary section reconstruct two aspects of comprising of system three-dimensional data.
7. laser confocal scanning micro imaging system according to claim 1, is characterized in that, described fluoroscopic image acquisition module is that the system acquisition image comprises image acquisition parameter setting and image acquisition two parts.
8. laser confocal scanning micro imaging system according to claim 7, it is characterized in that, described image acquisition parameter setting comprises following one or more: the setting of image size, image picture pitch setting, sweep limit setting, laser power setting, spatial filter setting, scan channel setting, sweep velocity setting and noise filter setting.
9. laser confocal scanning micro imaging system according to claim 7, is characterized in that, described image acquisition comprise following one or more: image prescan, image Single Slice Mode, image Multi Slice Mode and image real time scan.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104122242A (en) * | 2014-08-05 | 2014-10-29 | 华南农业大学 | Method for detecting agila formation ratio of agilawood |
| CN110146207A (en) * | 2019-05-16 | 2019-08-20 | 广东镭奔激光科技有限公司 | The depth of measurement residual stress is electrolysed etching system and method automatically |
| CN110162351A (en) * | 2019-03-12 | 2019-08-23 | 南昌大学 | A kind of software systems of 3-D scanning optical microscopy imaging |
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| CN101788484A (en) * | 2010-02-23 | 2010-07-28 | 中国人民解放军第四军医大学 | Chlorine imaging method of central nervous system based on laser confocal scanning microscope system |
| CN102841083A (en) * | 2012-06-11 | 2012-12-26 | 北京大学 | Method and system of laser scanning phase-microscope imaging |
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| CN101788484A (en) * | 2010-02-23 | 2010-07-28 | 中国人民解放军第四军医大学 | Chlorine imaging method of central nervous system based on laser confocal scanning microscope system |
| CN102841083A (en) * | 2012-06-11 | 2012-12-26 | 北京大学 | Method and system of laser scanning phase-microscope imaging |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122242A (en) * | 2014-08-05 | 2014-10-29 | 华南农业大学 | Method for detecting agila formation ratio of agilawood |
| CN104122242B (en) * | 2014-08-05 | 2016-06-15 | 华南农业大学 | A kind of detection method of Lignum Aquilariae Resinatum Edgeworthia chrysantha Lindl. rate |
| CN110162351A (en) * | 2019-03-12 | 2019-08-23 | 南昌大学 | A kind of software systems of 3-D scanning optical microscopy imaging |
| CN110162351B (en) * | 2019-03-12 | 2022-10-11 | 南昌大学 | Software system for three-dimensional scanning optical microscopic imaging |
| CN110146207A (en) * | 2019-05-16 | 2019-08-20 | 广东镭奔激光科技有限公司 | The depth of measurement residual stress is electrolysed etching system and method automatically |
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