CN110162351A - A kind of software systems of 3-D scanning optical microscopy imaging - Google Patents
A kind of software systems of 3-D scanning optical microscopy imaging Download PDFInfo
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Abstract
The present invention provides a kind of software systems of 3-D scanning optical microscopy imaging, the software systems mainly include data acquisition module, display module, control module, data memory module；The software systems are developed using VC++ based on MFC in VS2013 translation and compiling environment；The data acquisition module includes photon collection part, nanometer displacement part；The nanometer displacement part includes two-dimensional nano displaced portion, three-dimensional manometer displaced portion；This software systems is conceived to writing for the imaging softwares of systems such as common laser scanning co-focusing microscope (LSCM), fluorescence lifetime imaging system (FLIM) and stimulated emission depletion microscope (STED), parameter setting mode is simple, simple interface is beautiful, humanized design had both met experiment demand, it can be realized again comprising institute's function in need, it is very suitable to low development cost, the condition quickly developed, the functional need under scientific and technological rapid development background has been complied with, the building and related application of optical microscope system are highly suitable for.
Description
Technical field
The present invention relates to micro-imaging software technology field, specially a kind of software system of 3-D scanning optical microscopy imaging
System.
Background technique
High-precision imaging is carried out to subcellular structure using optical microscopy, due to the advantages such as lossless, real-time, one
Be directly life science, cell biology, nanometer material science main research means.But the common laser co-focusing in laboratory is aobvious
Micro mirror (Laser Scanning Confocal Microscopy, LSCM) business system is expensive, limits it in life
Science is widely used.Meanwhile the scientific research purpose based on different needs, such as realize the superelevation for surmounting optical diffraction limit
The super-resolution optical imaging system of spatial resolution is frequently necessary to change optical path or modernization system, uses the quotient of high concentration
Industry system is unfavorable for the development of such experiment.In addition, people pursue be imaged high-resolution while, to the intelligence of imaging system
Energyization control, realization have higher want to the flexible setting for parameters, image quality, system stability etc. of imaging software system
It asks.Therefore, independently building scanning imagery microscope and corresponding software system development are adapted to different requirements, can also save
About cost is conducive to push being widely used for high-resolution optical micro-imaging.
Summary of the invention
The purpose of the present invention is to provide a kind of software systems of 3-D scanning optical microscopy imaging, can be not only used for structure
Laser confocal microscope system (LSCM) is built, also can be directly used for fluorescence lifetime imaging system (Fluorescence
Lifetime Imaging Microscopy, FLIM) and other super-resolution imaging technologies, such as stimulated emission depletion microscope
The system development of (Stimulated Emission Depletion microscopy, STED) pushes optical microscopy imaging skill
Art promotes the development of related fields in the application in the fields such as life science, nano science, has important practical application value.
To achieve the above object, the invention provides the following technical scheme: a kind of software of 3-D scanning optical microscopy imaging
System, the software systems mainly include data acquisition module, display module, control module, data memory module；
The software systems are developed using VC++ based on MFC in VS2013 translation and compiling environment；
The data acquisition module includes photon collection part, nanometer displacement part, realizes photon collection and nanometer displacement
Precise synchronization control, using gradually move gradually record single pixel photon number by the way of scan each pixel, realize single
The number of photons record of a pixel time consistency mobile with location of pixels；The nanometer displacement part includes two-dimensional nano displacement portion
Divide, three-dimensional manometer displaced portion；The movement of the two-dimensional nano displaced portion has the three of lateral XY scanning and longitudinal direction XZ, YZ scanning
Kind mode；The three-dimensional manometer displaced portion is to control nanometer translation stage to carry out two Dimensional XY scanning in the Z axis position arbitrarily set；
The display module include image show, number of photons real-time display part, parameter real-time display；
The control module mainly includes parameter setting part, start-up and shut-down control part；
The data memory module is divided into image storage and data storage.
Further, the photon collection part is made of single-photon detector and photon collection card, acquires each imaging
The photon information of pixel realizes photon counting, and the nanometer displacement part is to control nanometer translation stage by computer, realizes
Accurate movement between Imaged samples and scanning lens in three dimensions.
Further, the software systems are realized by way of software code to photon collection card, nanometer translation stage
Time synchronization control；
The time synchronization control is called by calling the corresponding interface function of photon collection card, nanometer translation stage to realize
The collection interface function of photon collection card obtains the photon numerical value of single, single pixel, calls the mobile interface letter of nanometer translation stage
Number realizes the movement of pixel position；As soon as the every movement position of nanometer translation stage, photon collection card obtain a photon numerical value,
One Qi Yiting, high temporal are synchronous.
Further, the start-up and shut-down control part calls hardware driving function to realize control by button message response function
's.
Further, the software system implementation method has: signal transmission and data acquisition, pixel grayscale calibration,
The storage of multi-thread programming, parameter setting and real-time display, data；
The signal transmission realizes transmission using SMA communication cable, and the signal is the pulse signal of single-photon detector；
The number of photons information for each pixel that the data collecting module collected arrives will be according to the trace interval of setting
It is saved in customized three-dimensional array correspondingly with the position of corresponding nanometer translation stage X, Y, Z three-dimensional space, it is described
Number of photons information shows the image for being used for OpenGl and data store；
Described image, which is shown, demarcates each imaging pixel according to the photon information of data acquisition module by OpenGl
Gray value reconstructs two dimensional image；The gray value is to be marked according to the size of photon numerical value according to 256 gray levels
It is fixed, it can use the corresponding relationship that following formula acquires:
Wherein, S represents the gray level of pixel, CcRepresent the pixel number of photons currently to be demarcated, CmiRepresent three dimensions
Minimum number of photons value, C in groupmaRepresent maximum photon numerical value in three-dimensional array；
The data acquisition module and display module are all made of multi-thread programming, and the multithreading includes two threads, and one
A parameter shows thread, a scanning thread；
The parameter setting part includes photon collection card parameter setting and nanometer translation stage relative parameters setting, the light
Sub- capture card parameter carries out passing ginseng setting in EditControl control by creation modeless dialog box；The nanometer translation
Platform relevant parameter is arranged by edit box control；The acquisition time of photon collection card single pixel can be set in the parameter setting part
Interval, the parameters of photon collection card, nanometer translation stage single moving step length, scanning range；
The real-time display includes number of photons real-time display and parameter real-time display, the number of photons real-time display by
TeeChart control realizes that the parameter real-time display is by edit box and progress bar come real-time display；The parameter is shown in real time
Show it is that independent thread of opening is realized；
The storage of the data includes image storage and initial data storage, and described image storage uses the shape of interface screenshotss
Formula saves as BITMAP format, and stores under current directory；The initial data storage is saved in current work with .TXT format
Make under catalogue, the initial data is stored in the photon information data in the three-dimensional array, is the knot of single image scanning
Fruit, after starting scanning next time, last scanning result will be capped.
Further, the software systems operational process can be divided into: software systems are initialized, the acquisition process of information, are adopted
The scanning movement of sampling point position, the operation of multithreading, the memory processing of photon information, image is shown and number of photons real-time display；
The software systems initialization is realized using the basic control of MFC, and the parameters of photon collection card: light are arranged first
Then it is mobile that nanometer translation stage is arranged in sub- capture card trace interval, TAC gain, TAC range, CFD threshold value, CFD bound
Range, moving step length；
Photonic pulsed signals are converted into electric impulse signal for single-photon detector by the acquisition process of the information, by SMA
Cable is input to time correlation single photon capture card, realizes the photon information acquisition of each pixel；The photon information acquisition
It is to be stored data into three-dimensional array according to the trace interval and corresponding X, Y, Z location of setting；
The scanning of the sampling point position is mobile to be realized by computer and nanometer translation stage, refers to that nanometer translation stage receives meter
Calculation machine order is precisely moved, and then realizes the scanning of three-dimensional space；
The operation of the multithreading are as follows: parameter shows that thread starts immediately after system initialization completion, closes software
Terminate when interface；The scanning thread starts primary two dimension or 3-D scanning figure after starting two dimension perhaps 3-D scanning
Terminate；
The photon information memory processing be FIFO buffer using be deposit take by the way of cached；
Described image shows and realizes in such a way that OpenGl is by described point, according to each pixel of data acquisition module
Number of photons demarcate the pixel value of each point, rebuild two dimensional image in conjunction with the three-dimensional array；The three-dimensional array is programming people
The customized system memory space of member, the software systems are a pair of the three-dimensional coordinate of nanometer translation stage and three-dimensional array position one
It answers, and stores corresponding photon numerical value on corresponding position, the gray value of corresponding position pixel is converted into image reconstruction；
The number of photons real-time display is realized using TeeChart ActiveX drafting FastLine；It is with photon collection part list
The number of photons of secondary acquisition be the longitudinal axis, using when time acquisition time interval as horizontal axis, the photon curve of real-time rendering, the Real-Time Optical
Sub- curve reflects present scanning position in real time, and the collected number of photons in unit interval, the TeeCart control makes
TeeChart8.ocx Active component need to be first installed before.
Further, the software systems operational process can be described as:
(1) nanometer translation stage and photon collection card are connected；
(2) parameters that setting button opens a modeless dialog box setting photon collection card are clicked；
(3) acquisition time interval, scanning range, the parameter of scanning step are set in control interface, it as needed can be with
Scanning starting position is set；
(4) arrangement above, which terminates i.e. system initialization, terminates；
(5) starting scanning can put open the ComBox control of control scanning, select scanning mode that can open scanning thread,
Execute corresponding image scanning process；
(6) in scanning process, user can see real-time number of photons curve, and single image scanning process terminates, and can control
The region OpenGl at interface processed shows scan image；
(7) user can click on save button, store the image and initial data of present scan.
Compared with prior art, the beneficial effects of the present invention are:
This software systems is conceived to common laser scanning co-focusing microscope (LSCM), fluorescence lifetime imaging system
(FLIM) it is write with the imaging software of systems such as stimulated emission depletion microscope (STED), parameter setting mode is simple, interface
Simple and beautiful, humanized design had not only met experiment demand, but also can realize comprising institute's function in need, was very suitable to low open
Cost is sent out, the condition quickly developed has complied with the functional need under scientific and technological rapid development background, has been highly suitable for optical microphotograph system
The building and related application of system.
Detailed description of the invention
Fig. 1 is present system surface chart；
Fig. 2 is two-dimensional scanning imaging schematic diagram of the present invention；
Fig. 3 is two-dimensional scanning imaging example effect picture of the present invention；
Fig. 4 is 3-D scanning imaging schematic diagram of the present invention；
Fig. 5 is present invention number of photons display interface Local map in real time；
Fig. 6 is present system flow chart；
Fig. 7 is present system initialization flowchart；
Fig. 8 is scanning imagery flow chart of the present invention；
Fig. 9 is that present system memory handles brief introduction figure.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is described in further detail.The specific embodiments are only for explaining the present invention technical solution described herein, and
It is not limited to the present invention.
Software systems proposed by the invention are developed using VC++ based on MFC in VS2013 translation and compiling environment.System is adopted
It is realized while being displayed data with the mode of multi-thread programming and scanned with three-dimensional space.Software systems are flat by control nanometer
Moving stage realizes that the position of three-dimensional space between fluorescent samples and object lens is mobile, to realize the three-dimensional spot scan of imaging system.Together
When, the photon pulse information of single-photon detector is received and is counted by software systems control photon collection card.This software systems can
It is adopted with scanning step, scanning range and the photon collection card single when movement of nanometer translation stage single being arranged in control interface
The time interval of collection.This software systems realizes two and three dimensions image reconstruction with OpenGl, realizes using TeeChart control real
When show number of photons.The preservation of this software systems is imaged as bitmap format, and preservation data are .txt format.
Provided software implementation method according to the present invention, first control interface setting scanning step as shown in Figure 1,
Then the parameters such as scanning range, single pixel acquisition time interval set scanning mode, click " starting scanning " button, start
Software image.Two-dimensional scanning imaging schematic diagram as shown in Fig. 2, system by the way of spot scan.With transverse direction (XY) two-dimensional scanning
For, Y-axis is first moved along X-axis in initial position, computer control nanometer translation stage, one pixel of every movement, photon collection
One acquisition time interval of CalCt records number of photons summation in the trace interval of software set, strong as the pixel
Angle value, and each pixel intensity value is stored into the corresponding position to customized core buffer three-dimensional array.According to setting
Scanning range and single pixel acquisition time interval, nanometer translation stage are scanned along X-direction, are continuously increased along X-axis step-length,
Until reaching the preset scanning range of X-direction.Then a step-length is moved along the y axis, repeats the scanning of X-axis.The every increase of Y-axis
One scanning step, X-axis complete a line scanning, form S-shaped scan path.Until X-direction and Y-direction all reach maximum model simultaneously
Until enclosing, i.e., two-dimensional scanning terminates.Fig. 3 gives with the two dimensional imaging example of 40 nano fluorescent beads and 90 nanogold particles.
Fig. 4 gives 3-D scanning imaging schematic diagram, and 3-D scanning on the basis of two-dimensional scanning, increases the movement of Z axis.Two
On the basis of dimension scanning, a width two dimensional image is completed in every scanning, and corresponding computer control Z-direction moves up or down one
The two dimensional image of different Z axis, is then layered superposition, reconstructs three-dimensional figure by a step-length, the Z axis scanning range until completing setting
Picture.
The three-dimensional array is the customized system memory space of programming personnel, and the software systems are nanometer translation stage
Three-dimensional coordinate and three-dimensional array position correspond, and corresponding photon numerical value is stored on corresponding position, in image reconstruction
It is converted into the gray value of corresponding position pixel.Reconstruction of two-dimensional images and single are swept when the three-dimensional array is used as the end of scan
The data retouched save.
Laterally the scanning range representative value of (XY) two-dimensional scanning is 10um*10um；Scanning step representative value is 0.1um；It adopts
Integrate time interval representative value as 0.01s.It should be understood, however, that scanning model according to different fluorescent samples and imaging requirements
It encloses, scanning step, acquisition time interval are not limited to above-mentioned representative value.
Provided software implementation method according to the present invention realizes that the reconstruction of two and three dimensions image is aobvious using OpenGl
Show.After the location information and the corresponding gray value of location information that extract each pixel in three-dimensional matrice, pass through described point
Mode, a pixel on each of three-dimensional array pixel corresponding software interface, gray value is also corresponding to it, each picture
Vegetarian refreshments, which is all completed, is presented a width flat image.
The real-time display of number of photons is realized using TeeChart control.The present invention has selected TeeChart control
Fastline picture mode realizes the real-time display function of number of photons.As shown in figure 5, each trace interval scans one
Point, and the number of photons of the real-time display pixel.With adding up for sweep time, point is linked to be curve and obtains real-time number of photons
Curve.
The gray value is demarcated, be can use following according to 256 gray levels according to the size of photon numerical value
The corresponding relationship that formula acquires:
Wherein, S represents the gray level of pixel, CcRepresent the pixel number of photons currently to be demarcated, CmiRepresent three dimensions
Minimum number of photons value, C in groupmaRepresent maximum photon numerical value in three-dimensional array.
OpenGl before use, need to first be installed under VS2013 environment OpenGl often use library (glut32.lib,
Glut32.dll, glut.h etc.).TeeChart control need to re-mount TeeChart8.ocx in Windows operating system before
Active control, and successful registration.
Provided software implementation method according to the present invention, the data storage of software are divided into image storage and data storage.
Image stores by the way of screenshot, clicks the button " saving image " of software design, saves as BITMAP format.Data storage
It is saved under work at present catalogue with .TXT format.Initial data is stored in the photon information in the customized three-dimensional array
Data.
The interface and specific implementation flow of this software systems are specifically introduced with reference to the accompanying drawing:
If Fig. 1 is software systems pictorial diagram.The upper left corner is ListBox control, is used for display system current state and parameter
Facilities；Intermediate black box is the interface OpenGl, for realizing the display of two dimensional image；The right is TeeChart control, is used
Change in real-time display number of photons.Lower-left is the real-time display of the parameters such as photon collection calorimeter digit rate；Centre can be shown every time
The information such as the maximum photon number of scanning；It is setting for the parameters such as System trace time interval, scanning step below TeeChart control
Set region；It is most beneath to save control area for the control start and stop of system and data.
As shown in fig. 6, being initialized first after software systems starting.After the completion of system initialization, setting system is joined
Number, after being provided with, judges whether parameter is arranged and is over, prevent omission.System is received by interactive interface later
Imaging is scanned to scan command is started.System completes a two-dimensional scanning and stops scanning.It is saved by human-computer interaction interface
Image data and initial data.
As shown in fig. 7, system initialization mainly includes the connection of nanometer translation stage and the starting of photon collection card；Nanometer is flat
The setting of the system parameters such as moving stage moving range, moving step length；Photon collection card trace interval, TAC range, gain, CFD
The setting of the parameters such as bound.
As shown in figure 8, scanning imagery process, the precise synchronization for mainly solving photon collection and nanometer displacement is controlled.First
The position zero of nanometer translation stage is carried out, and starts the total number of light photons in photon collection CalCt certain time interval, is judged
Whether gate time reaches preset value, if reaching preset time value, the mobile scanning step of nanometer translation stage.Judge simultaneously
Whether nanometer translation stage is moved to preset maximum magnitude, if not reaching preset range, nanometer translation stage is moved to new
Position, total number of light photons and storage in the identical time interval of photon collection CalCt.It is single using gradually mobile gradually record
The mode of pixel light subnumber scans each pixel, and whole process is repeated up to always a nanometer translation stage and is moved to preset maximum
Range.After the end of scan, software systems start OpenGl and carry out two dimensional image reconstruction, and are shown to interface.
As shown in figure 9, the memory of software systems using FIFO caching by the way of obtain, using i.e. deposit take by the way of unloading
To the buffer area three-dimensional array of internal custom, then system extract the data in three-dimensional array carry out two dimensional image reconstruction and
Data save.
The above only expresses the preferred embodiment of the present invention, and the description thereof is more specific and detailed, but can not be because
This and be interpreted as limitations on the scope of the patent of the present invention.It should be pointed out that for those of ordinary skill in the art,
Under the premise of not departing from present inventive concept, several deformations can also be made, improves and substitutes, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (7)
1. a kind of software systems of 3-D scanning optical microscopy imaging, it is characterised in that: the software systems mainly include data
Acquisition module, display module, control module, data memory module；
The software systems are developed using VC++ based on MFC in VS2013 translation and compiling environment；
The data acquisition module includes photon collection part, nanometer displacement part, realizes the essence of photon collection and nanometer displacement
True synchronously control, using gradually move gradually record single pixel photon number by the way of scan each pixel, realize single picture
The number of photons record of the element time consistency mobile with location of pixels；The nanometer displacement part include two-dimensional nano displaced portion,
Three-dimensional manometer displaced portion；The movement of the two-dimensional nano displaced portion has three kinds of lateral XY scanning and longitudinal direction XZ, YZ scanning
Mode；The three-dimensional manometer displaced portion is to control nanometer translation stage to carry out two Dimensional XY scanning in the Z axis position arbitrarily set；
The display module include image show, number of photons real-time display part, parameter real-time display；
The control module mainly includes parameter setting part, start-up and shut-down control part；
The data memory module is divided into image storage and data storage.
2. a kind of software systems of 3-D scanning optical microscopy imaging according to claim 1, it is characterised in that: the light
Sub- collecting part is made of single-photon detector and photon collection card, acquires the photon information of each imaging pixel point, realizes light
Sub-count, the nanometer displacement part are to control nanometer translation stage by computer, are realized between Imaged samples and scanning lens
Accurate movement in three dimensions.
3. a kind of software systems of 3-D scanning optical microscopy imaging according to claim 1, it is characterised in that: described soft
Part system realizes the time synchronization control to photon collection card, nanometer translation stage by way of software code；
The time synchronization control calls photon by calling the corresponding interface function of photon collection card, nanometer translation stage to realize
The collection interface function of capture card obtains the photon numerical value of single, single pixel, calls the mobile interface function of nanometer translation stage real
The movement of phenomenon vegetarian refreshments position；As soon as the every movement position of nanometer translation stage, photon collection card obtain a photon numerical value, one is opened
One stops, and high temporal is synchronous.
4. a kind of software systems of 3-D scanning optical microscopy imaging according to claim 1, it is characterised in that: described to open
Stop control section and calls hardware driving function to realize control by button message response function.
5. a kind of software systems of 3-D scanning optical microscopy imaging according to claim 1, it is characterised in that: described soft
Part network system realization has: signal transmission and data acquisition, the calibration of pixel grayscale, multi-thread programming, parameter setting and reality
When display, data storage；
The signal transmission realizes transmission using SMA communication cable, and the signal is the pulse signal of single-photon detector；
The number of photons information for each pixel that the data collecting module collected arrives will be according to the trace interval of setting and right
The position for nanometer translation stage X, Y, Z three-dimensional space answered is saved in customized three-dimensional array, the photon correspondingly
Number information shows the image for being used for OpenGl and data store；
Described image shows the gray scale for demarcating each imaging pixel according to the photon information of data acquisition module by OpenGl
Value, reconstructs two dimensional image；The gray value is to be demarcated according to the size of photon numerical value according to 256 gray levels, can
Corresponding relationship to be acquired using following formula:
Wherein, S represents the gray level of pixel, CcRepresent the pixel number of photons currently to be demarcated, CmiIt represents in three-dimensional array
Minimum number of photons value, CmaRepresent maximum photon numerical value in three-dimensional array；
The data acquisition module and display module are all made of multi-thread programming, and the multithreading includes two threads, a ginseng
Digital display timberline journey, a scanning thread；
The parameter setting part includes photon collection card parameter setting and nanometer translation stage relative parameters setting, and the photon is adopted
Truck parameter carries out passing ginseng setting in EditControl control by creation modeless dialog box；The nanometer translation stage phase
Parameter is closed to be arranged by edit box control；The parameter setting part can be set between the acquisition time of photon collection card single pixel
Every, the parameters of photon collection card, nanometer translation stage single moving step length, scanning range；
The real-time display includes number of photons real-time display and parameter real-time display, and the number of photons real-time display is by TeeChart
Control realizes that the parameter real-time display is by edit box and progress bar come real-time display；The parameter real-time display is independent
Open what thread was realized；
The storage of the data includes image storage and initial data storage, and described image storage uses the form of interface screenshotss,
BITMAP format is saved as, and is stored under current directory；The initial data storage is saved in work at present with .TXT format
Under catalogue, the initial data is stored in the photon information data in the three-dimensional array, be single image scanning as a result,
After starting scanning next time, last scanning result will be capped.
6. the software systems of 3-D scanning optical microscopy imaging according to claim 1, it is characterised in that: the software system
System operational process can be divided into: software systems initialization, the scanning of the acquisition process of information, sampling point position are mobile, multithreading
Operation, the memory processing of photon information, image is shown and number of photons real-time display；
The software systems initialization realizes that the parameters of photon collection card are arranged first: photon is adopted using the basic control of MFC
Then the mobile model of nanometer translation stage is arranged in truck trace interval, TAC gain, TAC range, CFD threshold value, CFD bound
It encloses, moving step length；
Photonic pulsed signals are converted into electric impulse signal for single-photon detector by the acquisition process of the information, by SMA wire cable
It is input to time correlation single photon capture card, realizes the photon information acquisition of each pixel；The described photon information acquisition be by
Trace interval and corresponding X, Y, Z location according to setting store data into three-dimensional array；
The scanning of the sampling point position is mobile to be realized by computer and nanometer translation stage, refers to that nanometer translation stage receives computer
Order is precisely moved, and then realizes the scanning of three-dimensional space；
The operation of the multithreading are as follows: parameter shows that thread starts immediately after system initialization completion, closes software interface
When terminate；The scanning thread starts primary two dimension after starting two dimension perhaps 3-D scanning or 3-D scanning figure is tied
Beam；
The photon information memory processing be FIFO buffer using be deposit take by the way of cached；
Described image shows and realizes in such a way that OpenGl is by described point, according to the light of each pixel of data acquisition module
Subnumber demarcates the pixel value of each point, rebuilds two dimensional image in conjunction with the three-dimensional array；The three-dimensional array be programming personnel from
The system memory space of definition, the software systems correspond the three-dimensional coordinate of nanometer translation stage and three-dimensional array position,
And corresponding photon numerical value is stored on corresponding position, the gray value of corresponding position pixel is converted into image reconstruction；
The number of photons real-time display is realized using TeeChart ActiveX drafting FastLine；It is to be adopted with photon collection part single
The number of photons integrated is the longitudinal axis, and using when time time interval of acquisition is horizontal axis, the photon curve of real-time rendering, the real-time photon is bent
Line reflects present scanning position in real time, the collected number of photons in unit interval, before the TeeCart control use
TeeChart8.ocx Active component need to be first installed.
7. the software systems of 3-D scanning optical microscopy imaging according to claim 1, it is characterised in that: the software system
System operational process can be described as:
(1) nanometer translation stage and photon collection card are connected；
(2) parameters that setting button opens a modeless dialog box setting photon collection card are clicked；
(3) acquisition time interval, scanning range, the parameter of scanning step are set in control interface, can be set as needed
Scanning starting position；
(4) arrangement above, which terminates i.e. system initialization, terminates；
(5) starting scanning can put open the ComBox control of control scanning, select scanning mode that can open scanning thread, execute
Corresponding image scanning process；
(6) in scanning process, user can see real-time number of photons curve, and single image scanning process terminates, can be on control circle
The region OpenGl in face shows scan image；
(7) user can click on save button, store the image and initial data of present scan.
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| CN113091606A (en) * | 2021-03-21 | 2021-07-09 | 西北工业大学 | Cross-scale micro-nano structure laser manufacturing detection system and control method |
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| CN114935815A (en) * | 2022-05-26 | 2022-08-23 | 南昌大学 | Microscopic imaging system and method based on scanning galvanometer and nano translation stage |
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| CN113091606A (en) * | 2021-03-21 | 2021-07-09 | 西北工业大学 | Cross-scale micro-nano structure laser manufacturing detection system and control method |
| CN113091606B (en) * | 2021-03-21 | 2022-11-11 | 西北工业大学 | Cross-scale micro-nano structure laser manufacturing detection system and control method |
| CN113485700A (en) * | 2021-07-07 | 2021-10-08 | 华中科技大学鄂州工业技术研究院 | Qt-based design method and device for opto-electro-mechanical modular control software |
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| CN114935815A (en) * | 2022-05-26 | 2022-08-23 | 南昌大学 | Microscopic imaging system and method based on scanning galvanometer and nano translation stage |
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