CN201440126U - Non-damage micro-detecting system with optical segregant detection device - Google Patents
Non-damage micro-detecting system with optical segregant detection device Download PDFInfo
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- CN201440126U CN201440126U CN2009201067709U CN200920106770U CN201440126U CN 201440126 U CN201440126 U CN 201440126U CN 2009201067709 U CN2009201067709 U CN 2009201067709U CN 200920106770 U CN200920106770 U CN 200920106770U CN 201440126 U CN201440126 U CN 201440126U
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Abstract
The utility model relates to a non-damage micro-detecting system with an optical segregant detection device. The non-damage micro-detecting system comprises an electrode ion/molecule information detection unit, an optical ion/molecule information detection unit, a data acquisition/amplification system, a programmable three-dimensional moving system and a computer auto-control/data processing system, belonging to the technical field of mechanical and electronic integration. The computer auto-control/data processing system can control the synergistic testing of the electrode ion/molecule information detection unit and the optical ion/molecule information detection unit simultaneously, and can record and process the data information of the two detection units; and the computer auto-control/data processing system can also only control the independent detection of the electrode unit or the optical unit. By deploying the optical ion/molecule information detection device, the ions/molecules inside and outside a sample can be measured and the like, so that the data obtained by the testing experiment can be more comprehensive, and the reliability of the data can be higher; the non-damage micro-detecting system can be suitable for the testing requirements in different industries and fields, thereby widening the application range of the tested data greatly and being beneficial for saving manpower, material resources, finance resources and cost.
Description
Technical field
The utility model relates to a kind of device of measuring the ion action message, particularly relate to and adopt ion to select electrode and the optical means non-damage micrometering system of information such as measuring samples ion concentration, movement rate and direction of motion simultaneously, belong to the light mechanical and electrical integration field.
Background technology
The non-damage micrometering system that utilizes microelectrode to measure, be under the control of computing machine, utilize ion to select electrode to measure the information such as ion concentration, movement rate and direction of motion of turnover sample in the non-damage mode that does not contact measured material.The kind of microelectrode has glass electrode, metal electrode, carbon filament electrode or the like, and ion and the molecule that can measure have H
+, Ca
2+, K
+, Na
+, Mg
2+, Cl
-, NH
4 +, NO
3 -And O
2, H
2O
2, CO
2, NO or the like, sample can be unicellular, many cells, organizer official rank living material, also can be non-living materials such as metal.Along with ion is selected the progressively perfect of being on the increase of type of electrodes and electronics systems and computer hardware software, the non-damage micrometering technology is widely applied to numerous areas such as life science, preclinical medicine, pharmacy and metal erosion research gradually.
Existing non-damage micrometering system technical equipment mainly comprises electrode ion information detecting unit, data acquisition/amplification system, three-dimensional motion system able to programme and computer controlled automatic/data handling system etc.Whole system is the three-dimensional motion by the computer control microelectrode, and the ion parameter of microelectrode collection is input to computer data processing system after signal amplifies, and is implemented in simultaneously in the measuring process microelectrode and measured material are monitored in real time.
The laser co-focusing pick-up unit is made up of Laser Scanning Confocal Microscope and femtosecond infrared laser two parts, is optical microscope and the combining of modern laser, highly sensitive Detection Techniques, scan control technology and microcomputer image processing technique, fluorescence and labelling technique.Can realize Ca at present
2+, Mg
2+, H
+, Na
+, K
+Isoionic real time imagery has functions such as three-dimensionalreconstruction and quantitative test.The laser co-focusing pick-up unit is that life science has been opened up the new way that a structure of observing the life living cells and specific molecular, ion biology change, and becomes strong research tool in the fields such as molecular cytobiology, Neuscience, pharmacology, science of heredity.
The fluorescence imaging pick-up unit is made up of fluorescent microscope, the CCD first-class part of making a video recording, and is the product that optical microscopy combines with technology such as fluorescence and marks.The fluorescence imaging detection technique is existing long historical, constantly obtains new development in recent years.It has characteristics such as fast imaging, can remedy the deficiency of laser co-focusing technology to a certain extent.
Except that above-mentioned two kinds of sub-pick-up units of optical fractionation, sub-detection technique of the optical fractionation of other types and device are are researched and developed.The common feature of the sub-pick-up unit of these optical fractionation is the basis of forming as device with microscope, the ion action message of test sample inside.And the microelectrode test operation of existing non-damage micrometering system also need carry out at microscopically, and the data that obtain are the ion information of sample outside.On existing non-damage micrometering system, add the sub-pick-up unit of optical fractionation, can detect by shared microscope, by computer control, realization electric signal and light signal are gathered simultaneously, obtain the real-time activity information of the inside and outside ion of sample simultaneously, its test data is more comprehensive, and reliability is higher, the test request that can adapt to different industries, different field also helps the financial resources cost that uses manpower and material resources sparingly.But the novel non-damage micrometering system that is equipped with the sub-pick-up unit formation of optical fractionation on traditional non-damage micrometering system yet there are no report.
The utility model content
The utility model provides a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation, comprising: electrode ion information detecting unit, optical ion/molecular information detecting unit, data acquisition/amplification system, three-dimensional motion system able to programme and computer controlled automatic/data handling system.
Specify each parts of system of the present utility model and structural relation below.
Described electrode ion information detecting unit comprises a contrast electrode, one or more microelectrode, liquid medium and detection receptacle, microelectrode adopts ion to select electrode, can be glass electrode, metal electrode, carbon filament electrode, fibre electrode or other selectivity, specificity electrode.Microelectrode quantity can be one or more, and the operator can select according to the actual needs of measuring.Contrast electrode ground connection, contrast electrode will maintain a certain distance apart from sample, to avoid interference measuring-signal.Contrast electrode, sample and microelectrode place liquid medium, and microelectrode requires the close-in measurement sample, preferably apart from sample 5-35 micron.
Described optical ion/molecular information detecting unit comprises microscope, optical information acquisition system and other support equipments, and this optical ion/molecular information detecting unit can be that laser co-focusing pick-up unit, fluorescence imaging pick-up unit or other are equipped with the sub-pick-up unit of microscopical optical fractionation.Wherein microscope is selected according to the requirement of optical ion/molecular information detecting unit, can be simple microscope or other optical microscope, both can manual focusing, and also can be by the motion controller automatic focusing.
Described data acquisition/amplification system comprises prime amplifier, differential amplifier, A/D converter and D/A converter, each ion detection signal is input to prime amplifier and differential amplifier successively, detection signal is handled through A/D converter and D/A converter respectively after entering differential amplifier, remove background voltage differential signal noise, the detected ion active signal amplification of microelectrode is input to computer controlled automatic/data handling system later on above 1000 times the most at last.
Described three-dimensional motion system able to programme comprises motion controller and three-dimensional stepper motor, motion controller is connected with the microscope of three-dimensional stepper motor and optical ion/molecular information detecting unit respectively, the motion controller receiving computer controls automatically/motion control instruction that data handling system is sent, on the one hand by of the three-dimensional motion control of three-dimensional stepper motor to microelectrode, make microelectrode gather the information of sample ion activity in the mode of three-dimensional motion, motion controller carries out automatic focusing to the microscope of optical ion/molecular information detecting unit on the other hand, monitors microelectrode and optical measurement process in real time.
Described computer controlled automatic/data handling system is meant the computing machine that has auto-control software and data processing software.Sense command dominant pole ion information detecting unit and optical ion/molecular information detecting unit that auto-control software sends begin detected activity simultaneously.Auto-control software passes to motion controller with microelectrode three-dimensional motion steering order, adopts programmable mode to realize the three-dimensional motion of microelectrode.Data processing software is realized the collection of the ion signal of electrode ion information detecting unit and optical ion/molecular information detecting unit, storage, analysis and is shown the recorded data parameter.Data processing software on computer screen, provide different detection units the parameter setting window, be used to write down the real-time video data of different detection units ion action message record window, be used to analyze recorded data analysis window etc.Simultaneously, will be transferred to computer controlled automatic/data handling system, realize real-time monitoring and image storage in two kinds of different testing processes of two detecting units from the microscopical real-time monitor message of optical ion/molecular information detecting unit.
Specify the circuit relation between each parts of total system of the present utility model below.
The microelectrode of electrode ion information detecting unit is fixed on the mechanical arm of three-dimensional stepper motor of three-dimensional motion system able to programme, is connected with the prime amplifier of data acquisition/amplification system.The D/A converter of data acquisition/amplification system is connected with computer controlled automatic/data handling system.Motion controller one end of three-dimensional motion system able to programme is connected with the microscope of three-dimensional stepper motor with optical ion/molecular information detecting unit respectively, and the other end is connected with computer controlled automatic/data handling system.The optical information acquisition system of optical ion/molecular information detecting unit directly is connected with computer controlled automatic/data handling system.
When utilizing a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation that sample is detected, can carry out microelectrode and optics dual mode to sample and measure the comprehensive ion information that obtains simultaneously, also can only select wherein a kind of mode to measure, expand the range of choice of sample greatly according to the sample situation.Sample both can be living materials such as unicellular, many cells, histoorgan and even whole biosome, also can be non-living materials such as metal, polymer composite.
The beneficial effects of the utility model are by being equipped with the sub-pick-up unit of optical fractionation, to have realized by microelectrode and two kinds of approach measuring samples of optical means ion information.Computer controlled automatic/data handling system while control electrode ion information detecting unit and optical ion/molecular information detecting unit are worked in coordination with test, can write down and handle the data message of two detecting units simultaneously, operation such as realized that the inside and outside ion of sample is measured simultaneously.Except that the dual mode cooperative, the non-damage micrometering system that has a sub-pick-up unit of the optical fractionation condition mode of choosing any one kind of them is per sample measured.These data that make test experiments obtain are more comprehensive, and reliability is higher, adapt to the test request of different industries, different field, expanded the range of application of test data greatly, also help the financial resources cost that uses manpower and material resources sparingly.
Description of drawings
Fig. 1 is that the non-damage micrometering system that has the sub-pick-up unit of optical fractionation is formed synoptic diagram.
Fig. 2 is the non-damage micrometering system circuit theory diagrams that have the sub-pick-up unit of optical fractionation.
Embodiment
Below in conjunction with accompanying drawing 1 and accompanying drawing 2 and embodiment the utility model is described further.
As shown in Figure 1, the non-damage micrometering system that has a sub-pick-up unit of optical fractionation comprises electrode ion information detecting unit 1, optical ion/molecular information detecting unit 2, data acquisition/amplification system 3, three-dimensional motion system able to programme 4 and computer controlled automatic/data handling system 5.
As shown in Figure 2, electrode ion information detecting unit 1 comprises a contrast electrode 1-1, one or more microelectrode 1-2, liquid medium 1-3 and detection receptacle 1-4, microelectrode 1-2 adopts ion to select electrode, can be glass electrode, metal electrode, carbon filament electrode, fibre electrode or other selectivity, specificity electrode.Optical ion/molecular information detecting unit 2 comprises microscope 2-1, optical information acquisition system 2-2 and other support equipments 2-3, microscope 2-1 selects according to the requirement of optical ion/molecular information detecting unit 2, can be simple microscope or other optical microscope, both can manual focusing, also can be by the motion controller 4-1 automatic focusing of three-dimensional motion system 4 able to programme.The microelectrode 1-2 of electrode ion information detecting unit 1 is fixed on the mechanical arm of three-dimensional stepper motor 4-2 of three-dimensional motion system 4 able to programme, is connected with the prime amplifier 3-1 of data acquisition/amplification system 3.The D/A converter 3-4 of data acquisition/amplification system is connected with computer controlled automatic/data handling system 5.Motion controller 4-1 one end of three-dimensional motion system 4 able to programme is connected with the microscope 2-1 of optical ion/molecular information detecting unit 2 with three-dimensional stepper motor 4-2 respectively, and the other end is connected with computer controlled automatic/data handling system 5.The optical information acquisition system 2-2 of optical ion/molecular information detecting unit 2 directly is connected with computer controlled automatic/data handling system 5.
Specify embodiment of the present utility model below in conjunction with accompanying drawing 1 and accompanying drawing 2.
When the non-damage micrometering system that has a sub-pick-up unit of optical fractionation when utilization detects, computer controlled automatic/data handling system 5 outputs to motion controller 4-1 with sense command, motion controller 4-1 controls three-dimensional stepper motor 4-2 respectively and drives microelectrode 1-2 and focus according to preset path motion and microscope 2-1, makes electrode ion information detecting unit 1 and optical ion/molecular information detecting unit 2 begin measurement to sample ions/molecule activity information simultaneously.The detection signal of microelectrode 1-2 is handled through prime amplifier 3-1, differential amplifier 3-2, A/D converter 3-3 and D/A converter 3-4 respectively, remove background voltage differential signal noise, the signal amplification is outputed to computer controlled automatic/data handling system 5 after 1000 times.The detection signal of microscope 2-1 also outputs to computer controlled automatic/data handling system 5 after optical information acquisition system 2-2 gathers and handles.Computer controlled automatic/data handling system 5 is handled two kinds of detection signals simultaneously, according to the real-time monitoring and detection process of detection signal, adjusts sense command in case of necessity and feeds back to motion controller 4-1.Situation per sample, computer controlled automatic/data handling system 5 can a control electrode ion information detecting unit 1 or optical ion/molecular information detecting unit 2 one detect separately and close another detecting unit simultaneously.
Above embodiment adopts the non-damage micrometering system that has the sub-pick-up unit of optical fractionation that the ion of sample is detected, compare with traditional detecting pattern and to have obvious practicability and technical progress, realized the collaborative test of microelectrode method and optical means on the one hand to the ion action message, operation such as can realize that the inside and outside ion of sample is measured simultaneously, the data that make test experiments obtain are more comprehensive, and reliability is higher; On the other hand, situation both can have been carried out the collaborative test of microelectrode method and optical means per sample, can select one to test separately again, adapted to the test request of different industries, different field, had expanded the range of application of test data greatly.Same system is equipped with two kinds of ion detection methods, also helps the financial resources cost that uses manpower and material resources sparingly.
Claims (8)
1. non-damage micrometering system that has the sub-pick-up unit of optical fractionation, comprise electrode ion information detecting unit (1), data acquisition/amplification system (3), three-dimensional motion system able to programme (4) and computer controlled automatic/data handling system (5), it is characterized in that, also comprise optical ion/molecular information detecting unit (2), wherein the microelectrode (1-2) of electrode ion information detecting unit (1) is fixed on the mechanical arm of three-dimensional stepper motor (4-2), be connected with computer controlled automatic/data handling system (5) with data acquisition/amplification system (3) respectively, motion controller (4-1) is connected with the microscope (2-1) of three-dimensional stepper motor (4-2) with optical ion/molecular information detecting unit (2) respectively; The optical information acquisition system (2-2) of optical ion/molecular information detecting unit (2) is connected with computer controlled automatic/data handling system (5); Electrode ion information detecting unit (1) and optical ion/molecular information detecting unit (2) detects the segregant information of sample respectively, be transferred to computer controlled automatic/data handling system (5), the test process of computer controlled automatic/data handling system (5) output control signal control electrode ion information detecting unit (1) and optical ion/molecular information detecting unit (2).
2. a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation according to claim 1, it is characterized in that, described electrode ion information detecting unit (1) comprises a contrast electrode (1-1), one or more microelectrode (1-2), liquid medium (1-3) and detection receptacle (1-4), microelectrode (1-2) adopts ion to select electrode, can be glass electrode, metal electrode, carbon filament electrode, fibre electrode or other selectivity, specificity electrode.
3. a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation according to claim 1, it is characterized in that, described optical ion/molecular information detecting unit (2) comprises microscope (2-1), optical information acquisition system (2-2) and other support equipments (2-3), and this optical ion/molecular information detecting unit can be that laser co-focusing pick-up unit, fluorescence imaging pick-up unit or other are equipped with the sub-pick-up unit of microscopical optical fractionation.
4. according to claim 1 or 3 described a kind of non-damage micrometering systems that have the sub-pick-up unit of optical fractionation, it is characterized in that, described microscope (2-1) is selected according to the requirement of optical ion/molecular information detecting unit (2), can be simple microscope or other optical microscope, both can manual focusing, also can be by motion controller (4-1) automatic focusing of three-dimensional motion system able to programme (4).
5. a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation according to claim 1, it is characterized in that, described sample can be to be fit to the sample that the microelectrode segregant detects and the detection of optical fractionation is carried out simultaneously, as the living cells sample, also can be the sample that only is fit to detect segregant information with microelectrode or a kind of method of optics.
6. a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation according to claim 1, it is characterized in that, the motion controller (4-1) of described three-dimensional motion system able to programme (4) is connected with three-dimensional stepper motor (4-2), and the microelectrode (1-2) that three-dimensional stepper motor (4-2) drives electrode ion information detecting unit (1) carries out three-dimensional motion in whole measuring process.
7. a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation according to claim 1, it is characterized in that, the prime amplifier (3-1) of described data acquisition/amplification system (3) is connected with differential amplifier (3-2), detection signal is handled through A/D converter (3-3) and D/A converter (3-4) respectively after entering differential amplifier (3-2) again, remove background voltage differential signal noise, output after the detected ion active signal amplification of the microelectrode (1-2) of electrode ion information detecting unit (1) surpasses 1000 times the most at last.
8. a kind of non-damage micrometering system that has the sub-pick-up unit of optical fractionation according to claim 1, it is characterized in that, described computer controlled automatic/data handling system (5) can write down and handle the data message of electrode ion information detecting unit (1) and optical ion/molecular information detecting unit (2) input simultaneously and can export the testing process of control signal while control electrode ion information detecting unit (1) and optical ion/molecular information detecting unit (2), the detection of electrode ion information detecting unit (1) and optical ion/molecular information detecting unit (2) is begun simultaneously, finish simultaneously, realize collaborative test.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103460026A (en) * | 2011-03-29 | 2013-12-18 | 奥林巴斯株式会社 | Photometric analysis device, photometric analysis method, and computer program for photometric analysis, using single light-emitting particle detection |
| CN103823029A (en) * | 2012-11-16 | 2014-05-28 | 旭月(北京)科技有限公司 | Method for judging food safety according to flow velocity of hydrogen ions |
| CN106908428A (en) * | 2017-03-02 | 2017-06-30 | 大连光耀辉科技有限公司 | A kind of Laser Scanning Confocal Microscope with electrochemistry imaging function |
| CN109387670A (en) * | 2017-08-07 | 2019-02-26 | 中国科学院沈阳自动化研究所 | A kind of SICM voltage modulated imaging device and method based on difference noise reduction |
| CN111912993A (en) * | 2019-05-07 | 2020-11-10 | 旭月(北京)科技有限公司 | High-throughput automatic rapid non-damage micrometering system and method |
| CN112964648A (en) * | 2021-03-23 | 2021-06-15 | 中国科学院力学研究所 | Single ion detection method and device |
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2009
- 2009-04-09 CN CN2009201067709U patent/CN201440126U/en not_active Expired - Lifetime
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103460026A (en) * | 2011-03-29 | 2013-12-18 | 奥林巴斯株式会社 | Photometric analysis device, photometric analysis method, and computer program for photometric analysis, using single light-emitting particle detection |
| CN103460026B (en) * | 2011-03-29 | 2015-06-10 | 奥林巴斯株式会社 | Photometric analysis device, photometric analysis method, and computer program for photometric analysis, using single light-emitting particle detection |
| CN103823029A (en) * | 2012-11-16 | 2014-05-28 | 旭月(北京)科技有限公司 | Method for judging food safety according to flow velocity of hydrogen ions |
| CN103823029B (en) * | 2012-11-16 | 2015-10-21 | 旭月(北京)科技有限公司 | A kind of method being differentiated foodsafety by hydrogen ion flow velocity |
| CN106908428A (en) * | 2017-03-02 | 2017-06-30 | 大连光耀辉科技有限公司 | A kind of Laser Scanning Confocal Microscope with electrochemistry imaging function |
| CN109387670A (en) * | 2017-08-07 | 2019-02-26 | 中国科学院沈阳自动化研究所 | A kind of SICM voltage modulated imaging device and method based on difference noise reduction |
| CN109387670B (en) * | 2017-08-07 | 2020-12-29 | 中国科学院沈阳自动化研究所 | SICM voltage modulation imaging device and method based on differential noise reduction |
| CN111912993A (en) * | 2019-05-07 | 2020-11-10 | 旭月(北京)科技有限公司 | High-throughput automatic rapid non-damage micrometering system and method |
| CN112964648A (en) * | 2021-03-23 | 2021-06-15 | 中国科学院力学研究所 | Single ion detection method and device |
| US11808695B2 (en) | 2021-03-23 | 2023-11-07 | Institute Of Mechanics, Chinese Academy Of Sciences | Single ion detection method and device |
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