CN102450973A - Multifunctional wet tissue containing orange essence - Google Patents
Multifunctional wet tissue containing orange essence Download PDFInfo
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- CN102450973A CN102450973A CN2011102870246A CN201110287024A CN102450973A CN 102450973 A CN102450973 A CN 102450973A CN 2011102870246 A CN2011102870246 A CN 2011102870246A CN 201110287024 A CN201110287024 A CN 201110287024A CN 102450973 A CN102450973 A CN 102450973A
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Abstract
The invention discloses a multifunctional wet tissue containing orange essence. The wet tissue is characterized by being prepared by pouring the orange essence and auxiliary materials into reverse osmosis (RO) purified water, mixing, uniformly stirring, standing, uniformly spraying the obtained mixed liquid to a spunlaced non-woven fabric, and hermetically packaging. According to a formula, the wet tissue comprises the following components in percentage by weight: 0.05 to 5 percent of orange essence, 0 to 10 percent of auxiliary materials and 85 to 99.95 percent of RO purified water. The wet tissue is safe and non-toxic, has a strong sterilization function and multiple functions, and is widely applied, and wet tissues suitable for various consumer groups can be prepared by adjusting the formula.
Description
Technical field
the present invention relates to a kind of multifunctional wet towel that contains orange essence.
Background technology
The primary raw material that
are made wet towel at present has following several types: the first kind is that medical material is pasteurization towelette and the wet sanitary napkins that primary raw material is made; Second type for chemical raw material is pasteurization towelette and the wet sanitary napkins that primary raw material is made, for example ozone etc.; The 3rd type is the wet towel that the not obvious toxicity of bactericidal effect is hanged down the supression bacterium of making for primary raw material.
in sum, existing product generally is not that toxicity is bigger, is exactly that unpleasant taste or bactericidal effect is undesirable, or anticorrisive agent is arranged, and the harmful material of chemosterilant or the like is perhaps arranged.
Wherein there is the natural materials-orange essence (CITRUS EXTRACT) with antimicrobial physiologically active in
citrous fruit.The sixties in last century; The Jacob Harich.Steven Otwell of instrument institute of microbiology of Univ Florida USA (famous Gainesville laboratory) and Wayne Marshall tiji research are extracted orange essence from citrous fruit; Wherein contain oranges and tangerines fruit elite, vitamin C, citric acid, amino acid, citrus pectin, glyceride; Mentioned component whole typing FDA in the sixth of the twelve Earthly Branches catalogue is the safety non-toxic material; Existing being widely used in the sixth of the twelve Earthly Branches in the world has the effect of antibiotic and sterilizing preferably in food, health care device, cosmetics and the medical industry.
Summary of the invention
the present invention seeks to: a kind of multifunctional wet towel that contains orange essence is provided, its high-efficiency low-toxicity or nontoxic, colourless, tasteless, do not have chemical harmful substance, pure natural is suitable for all kinds of crowds' demand.
Technical scheme of the present invention is: a kind of multifunctional wet towel that contains orange essence; It is characterized in that orange essence and auxiliary material poured in the RO pure water and mix, stir, static; Then the gained mixed liquor evenly is sprayed on the spunlace non-woven cloth, packs and promptly get the towel that wets; Mixture formula is following:
Component content (weight %)
Orange essence 0.05-5%;
Auxiliary material 0-10%;
RO pure water 85-99.95%.
above-mentioned multifunctional wet towel that contains orange essence; It is characterized in that described orange essence is the product that from oranges and tangerines, extracts, main component is oranges and tangerines fruit elite, vitamin C, citric acid, amino acid, citrus pectin, glyceride and glycerine.
The multifunctional wet towel that contains orange essence that
are above-mentioned is characterized in that described auxiliary material is natural materials such as natural glycerin, hyaluronic acid, pyrrolidone sodium carboxylate, aloe.
Advantage of the present invention is:
1. the present invention have the strong disinfecting function; Through the contact microorganism; Change its cell membrane permeability; Stop its aerobic respiration to reach bactericidal effect,, Candida albicans unicellular to staphylococcus aureus, Escherichia coli, verdigris vacation through detecting has killing action rapidly.
. safety non-toxic of the present invention, the primary raw material orange essence is a pure natural plant extract, through the abnormal test of skin, safety non-toxic is confirmed in skin irritatin reaction test and acute skin test.
. the present invention is economical and practical; China is an oranges and tangerines plantation big country; Along with the development of orange essence product, opportunity to develop is provided for the oranges and tangerines deep processing, guaranteed supply of raw material; So the pasteurization towelette of employing orange essence sterilization and existing pasteurization towelette price are with very nearly the same, cost is very reasonable.
diverse in function of the present invention is regulated prescription and can be processed the wet towel that is fit to various consumer groups' uses, is widely used.
Below in conjunction with embodiment the present invention is further described:
specific embodiment.
Embodiment 1:
The mixed liquor proportioning:
(1) orange essence 0.8g
(2) RO pure water 500ml
100 of spunlace non-woven cloths (150mm*200mm) (about 160g)
Mix orange essence
with the RO pure water, stirred 30 minutes, static.Then the gained mixed liquor is sprayed on 100 blocks of nonwoven equably package encapsulation.
Embodiment 2:
The mixed liquor proportioning:
(1) orange essence 0.8g
(2) RO pure water 500ml
(3) natural glycerin 2ml
100 of spunlace non-woven cloths (150mm*200mm) (about 160g)
Mix orange essence and natural glycerin
with the RO pure water, stirred 30 minutes, static.Then the gained mixed liquor is sprayed on 100 blocks of nonwoven equably package encapsulation.
Embodiment 3:
The mixed liquor proportioning:
(1) orange essence 1.25g
(2) RO pure water 500ml
100 of spunlace non-woven cloths (150mm*200mm) (about 160g)
Mix orange essence
with the RO pure water, stirred 30 minutes, static.Then the gained mixed liquor is sprayed on 100 blocks of nonwoven equably package encapsulation.
Embodiment 4:
The mixed liquor proportioning:
(1) orange essence 1.20g
(2) RO pure water 500ml
(3) pyrrolidone sodium carboxylate 2.5ml
(4) Sodium Hyaluronate 0.5g
100 of spunlace non-woven cloths (150mm*200mm) (about 160g)
are dissolved in sodium hyaluronate in the pure water earlier, stir after adding pyrrolidone sodium carboxylate and orange essence again, after spraying the 5ml feed liquid uniformly on every block of nonwoven, pack.
Embodiment 5:
The mixed liquor proportioning:
(1) orange essence 5g
(2) RO pure water 500ml
100 of spunlace non-woven cloths (150mm*200mm) (about 160g)
Mix orange essence
with the RO pure water, stirred 30 minutes, static.Then the gained mixed liquor is sprayed on 100 blocks of nonwoven equably package encapsulation.
Below be test to embodiment:
One, to the test of embodiment 1 and 2
embodiment 1 and 2 is mainly used in general wet towel and baby's wet paper handkerchief, and the target customer is general crowd and infant.The concrete test as follows:
1, microbial contamination detects
1.1 product collection and sample treatment
Each 12 minimum marketing packing sample is extracted at least in
in embodiment 1 and 2 packings, 1/4 sample is used for detecting, and 1/4 sample is used to keep sample, and 1/2 Yang Ping (can seal up for safekeeping on the spot) is used for rechecking in case of necessity in addition.The minimum marketing packing of sampling should be by not breaking, must not breakdown before the check.
are opened at least 3 of being used to detect with aseptic method and are packed under 100 grades of purification conditions, sampling from each packing accurately takes by weighing 10g ± 1g sample.Shred the back and add in the 200ML sterile saline, fully mixing obtains a physiological saline appearance liquid.Fluid product is directly done a kind liquid with stoste.
A large amount of absorbing resin material are contained like test sample in
and when causing can not sucking-off enough appearance liquid, the each 50ML of dilution liquid measure increases progressively, until sucking-off enough testing with an appearance liquid.When calculating total number of bacterial colonies and fungus colony sum, should adjust dilution factor.
Total number of bacterial colonies and initial contaminated bacteria detection mode
this method is applicable to that the initial contaminated bacteria of product and total number of bacterial colonies (below be referred to as total number of bacterial colonies) detect.
Operating procedure
treat that getting supernatant after the above-mentioned physiological saline appearance liquid natural subsidence does colony counting.Inoculate 5 plates altogether, add 1ML appearance liquid in each plate, then with mixing in each plate of nutrient agar 15-20ML importing that is cooled to the fusing about 45 ℃.After treating that agar solidifies back upset plate to 35 ℃ ± 2 ℃ and cultivates 48h, calculate the clump count on the flat board.
Result's report
The flat board that bacterium colony is grown in the form of sheets should not adopt, and counts the bacterium colony on the satisfactory flat board, presses formula (B1) result of calculation:
X1=AK/5...................................................(B1)
In the formula: X1----total number of bacterial colonies, cfu/g or cfu/mL:
Total number of bacterial colonies on A---5 nutrient agar flat boards;
K---dilution factor.
exist when clump count: in 100, by real the number report arranged, greater than adopting two position effective digitals at 100 o'clock.
are if the sample total plate count surpasses the regulation of this standard. and recheck with the result by 1.2.3 and report.
The reinspection method
The reinspection sample that
will retain is according to preceding method repetition measurement 2 times, 2 times as a result mean value all reach the regulation of this standard, judge that then test sample is qualified; Wherein have any 1 time as a result mean value surpass this standard code, judge that then test sample is defective.
1.3 coliform detection method
1.3.1 operating procedure
sampling liquid 5mL. inoculation 50ml, bile salt lactose fermentation tube is put 35 ℃ ± 2 ℃ and is cultivated 24h, as not producing also aerogenesis not of acid, then is reported as the coliform feminine gender.
as producing sour aerogenesis, then the streak inoculation eosin methylene blue agar is dull and stereotyped, puts 35 ℃ ± 2 ℃ and cultivates 18～24h, observes the dull and stereotyped colonial morphology of going up.Typical large intestine bacterium colony is black purple or reddish violet, circular, neat in edge, and smooth surface is moistening, often has metallic luster, and what also have is atropurpureus, is not with or has a little metallic luster, or pink, the bacterium colony that the center is darker.
are got 1～2 of doubtful bacterium colony and are made gram stain microscopy, inoculate lactose fermentation tube simultaneously, put 35 ℃ ± 2 ℃ and cultivate 24h, observe the aerogenesis situation.
Result's report
all bile salt lactose fermentation tubes produce sour aerogenesis, and lactose fermentation tube produces sour aerogenesis, and typical large intestine bacterium colony is arranged on the methylene blue flat board of Yihong, and the negative bactacin of Gram can report that test sample detects Escherichia coli.
The Pseudomonas aeruginosa detection mode
1.4.1 operating procedure
sampling liquid 5ml joins 50m1, and in the SCDLP nutrient solution, fully mixing is put 35 ℃ ± 2 ℃ and cultivated 18～24h.If any the Pseudomonas aeruginosa growth, the nutrient solution surface presents the thin mycoderm of one deck, and nutrient solution often is yellow green or blue-green.From the thin mycoderm picking culture of nutrient solution, streak inoculation hexadecane trimethyl ammonium bromide agar plate is put 35 ℃ ± 2 ℃ and is cultivated 18～24h, observes colony characteristics.Pseudomonas aeruginosa is well-grown on this culture medium, and bacterium colony is flat, and the edge is not whole, and the periphery of bacterial colonies culture medium has a little pink, and other bacterium are not long.
Get suspicious bacterium colony smear and make Gram, microscopy should carry out following test for the Gram-negative bacteria person:
oxidase test: get the clean white filter paper of a fritter and be placed in the sterilization plate; Be coated on the filter paper with the suspicious bacterium colony of aseptic glass rod picking; Drip 1% dimethyl-p-phenylenediamine's test solution of a new preparation then above that; Occur pink or aubergine in the 30s, positive for oxidase test, nondiscolouring person is negative.
pyo test: get 2～3 suspicious bacterium colonies; Be seeded in pyo respectively and measure, add chloroform 3～5mL with medium slant .35 ℃ ± 2 ℃ cultivation 24h; Fully vibration makes the pyo dissolving that possibly exist in the culture; When treating that three oxygen methane are blueness, with suction pipe move on in another test tube and add lmol/L hydrochloric acid lm [., leave standstill after the vibration a moment.Pink occurs or aubergine is promptly positive like the upper strata, expression has pyo to exist.
The test of
nitrate reduction aerogenesis: the seized bacterium colony pure culture of picking is seeded in the boundless hydrochlorate and dips in the water culture medium, puts 35 ℃ ± 2 ℃ and cultivates 24h, and the person is promptly positive to have gas in the little voltage regulator tube of culture medium.
gelatin liquefaction test: get suspicious bacterium colony pure culture, percutaneous puncture-inoculation is put 35 ℃ ± 2 ℃ cultivation 24h taking-up and is put in 4 ~ 10 ℃ in gelatin culture medium, and positive as still being in a liquid state, the person of solidifying is negative.
℃ growth test: get suspicious culture, be seeded on the plain agar slant medium, put 42 ℃ and cultivate 24-48h, have Pseudomonas aeruginosa to be grown to the positive.
Result's report
test sample is after increasing the bacterium separation and Culture, and demonstrate,proving stuttering is gram-Negative bacillus, and oxidizing ferment and all positive person of Pseudomonas aeruginosa test can report to detect Pseudomonas aeruginosa in the test sample.Like pyo test feminine gender and liquefy gelatin, nitrate reduction aerogenesis and 42 ℃ of growth test threes when being all the positive still can report to detect Pseudomonas aeruginosa in the test sample.
Detection methods of staphylococcus aureus
1.5.1 operating procedure
sampling liquid 5mL. joins in the 50mL SCDLP nutrient solution, and fully mixing is put 35 ℃ ± 2 ℃ 24h.
L～2 oeses are got in
in above-mentioned enrichment liquid, streak inoculation is put 35 ~ C ± 2 ℃ cultivation 24～48h on blood agar culture-medium.This bacterium colony is golden yellow on blood agar plate, and is big and projection is circular, opaque, and smooth surface has the haemolysis circle on every side.
picking colonies typical, smear is made gram stain microscopy, and staphylococcus aureus is a gram-positive cocci, is arranged in botryoidalis, no brood cell and pod membrane.Microscopy meets above-mentioned situation, should carry out following test:
The test of
mannose ferment: get above-mentioned colony inoculation sweet mellow wine nutrient solution, put 35 ℃ ± 2 ℃ and cultivate 24h, it is positive that fermentation sweet mellow wine produces sour person.
coagulase test of blood plasma: slide method: get the clean dry slide; One end drips a physiological saline; The other end drips a rabbit plasma, and picking colony mixes with physiological saline and blood plasma respectively, occurs agglomerate or graininess grumeleuse in 5min such as the blood plasma; The salt water droplet still be even muddy do not have solidify then positive, as both all do not have solidify then negative.All salt water droplets and blood plasma drip solidification phenomenon are all arranged, and carry out the test tube coagulase butter again; Test tube method: draw l:4 fresh plasma 0.5mL, put in the sterilization small test tube, add equivalent bacterium 24h to be checked broth culture 0.5mL.Mixing is put in 35 ℃ ± 2 ℃ incubators or the water-bath, and observe once per half an hour, and it is promptly positive to present grumeleuse within the 24h.Simultaneously with known clotting of plasma enzyme positive and each 0.5mL of negative strain broth culture.As the positive and negative control.
Result's report
all to have suspicious colony growth on agar plate, microscopy is a gram-positive staphylococcus, and the sweet mellow wine that can ferment produces acid, and coagulase test of blood plasma positive person can report that test sample detects staphylococcus aureus.
The hemolytic streptococcus detection method
1.6.1 operating procedure
sampling liquid 5mL joins the 50mL dextrose bouillon, cultivates 24h for 35 ℃ ± 2 ℃.
are cultivated culture streak inoculation blood agar plate 24h for 35 ℃ ± 2 ℃ and are observed colony characteristics.Hemolytic streptococcus is canescence flat the pulling of blood, and is translucent or opaque, the tip-like projection, and smooth surface, neat in edge has the colourless haemolysis circle of chewing on every side.
picking colonies typical is made gram stain smear microscopy, should be Gram-positive, is the coccus of catenation.Microscopy meets above-mentioned situation should carry out following test:
The test of
streptokinase: absorption potassium oxalate blood plasma is (the 0.Olg potassium oxalate adds 5mL rabbit plasma mixing, through centrifugation, draws supernatant) o.2mL; Add the 0.8mL sterile saline; Add again behind the mixing bacterium 24h broth culture to be checked O.5mL with O25% calcium chloride 0.25ml, mixing is put in 35 ℃ ± 2 ℃ water-baths; 2min observes once (solidifiable in the general 10min), treats that clotting of plasma continued is observed and the record solution time.As not dissolving in the 2h, continue to place 24h and observe, positive like the grumeleuse off-bottom, the still insoluble feminine gender that turns to of 24h.
bacitracin sensitization test: seized bacterium bacterium liquid is applied on the blood plate; Getting the every scraps of paper that contain 0.04 unit pavilion bacterium tripe with the sterilization tweezers is placed on the planar surface; Know that with oneself positive strain compares simultaneously; At 35 ℃ ± 2 ℃ held 18～24h, there is button Han band person positive.
Result's report
microscopy Gram-positive catenation coccus presents molten _ blood circle on the blood plate, streptokinase and bacitracin test are positive, can report that test sample detects hemolytic streptococcus.
Fungus colony sum detection method
1.7.1 operating procedure
are treated to get after the above-mentioned physiological saline appearance liquid natural subsidence supernatant and are made the fungi counting.Inoculate 5 plates altogether; Add 1mL appearance liquid in each plate, fall with the sabouraud's agar 15～25mL that is cooled to the fusing about 45 ℃ then and mix in each plate of people, agar solidifies back upset plate to be put 25 ℃ ± 2 ℃ and cultivated 7 days; Observed respectively at 3,5,7 days; Calculate the clump count on the flat board,, be as the criterion with previous colony counting if find that bacterium colony spreads.
Result's report
The flat board that bacterium colony is grown in the form of sheets should not adopt: count the bacterium colony on the satisfactory flat board, press formula (B2) result of calculation:
X2=B*K/5.................................................（B2)
In the formula: X2---the fungus colony sum, cfu/g or cfu/mL:
Fungus colony sum on B---5 sabouraud's agar flat boards;
The K----dilution factor
when clump count in 100, by real the number report arranged, greater than adopting two position effective digitals at 100 o'clock.
are the total regulation that surpasses this standard if the sample mattress falls, and rechecks with the result by B7.3 and reports.
The reinspection method
The reinspection sample that
will retain is complied with preceding method repetition measurement 2 times, and 2 results reach the regulation of this standard, judge that then test sample is qualified: wherein have any 1 result to surpass this standard code, judge that then test sample is defective.
The fungi qualitative checking method
1.8.1 operating procedure
sampling liquid 5mL joins in the 50mL sabouraud culture medium, cultivates 7 days for 25 ℃ ± 2 ℃, observes day by day to have or not conk.
Result's report
culture tube muddiness should be changeed kind of a sabouraud's agar, and confirming has conk, can report that test sample detects fungi.
The microbial contamination testing result
| Detect index | Standard value | Measured value |
| Total number of bacterial colonies (cfg/g) | ≤200 | ＜20 |
| Fungi | Must not detect | Do not detect |
| Coliform | Must not detect | Do not detect |
| Pseudomonas aeruginosa | Must not detect | Do not detect |
| Staphylococcus aureus | Must not detect | Do not detect |
| Hemolytic streptococcus | Must not detect | Do not detect |
1.10 conclusion
The microbial contamination testing result of
this wet towel meets the regulation of " disposable use amenities sanitary standard " GB15979-2002.
, bacteriostatic test
2.1 sample is collected
have good representativeness for sample is had, and from each embodiment packing, randomly draw 20 minimum marketing packing samples at least, and wherein 5 keep sample, and do antibacterial or the bactericidal property test, do stability test for 10 for 5.
Test organisms and the preparation of bacterium liquid
2.2.1 test organisms
2.2.1.1 bacterium: staphylococcus aureus (ATCC 6538), Escherichia coli (8099 or ATCC 25922).
saccharomycete: Candida albicans (ATCC 10231).
bacterium liquid preparation: get the bacterial strain nutrient agar inclined-plane fresh cultured thing (18～24h) in the 3rd～14 generation; (hereinafter to be referred as PBS) washes lawn with the 5ml0.03mol/L phosphate buffer, and the back is diluted to desired concn with above-mentioned PBS to make bacterium suspend evenly.
Non-dissolubility resists (pressing down) bacterium product bacteriostasis property test method
2.3.1 operating procedure
take by weighing by coupons (being cut into the 1.0cmX1.0cm size) 0.75g packing and wrap.
add same of 0.75g in the conical flask of a 250mL, add 70mL PBS and 5 letter bacteria suspensions respectively, and making bacteria suspension is 1 * 104～9 * 104cfu/ml in the concentration of PBS article.
are fixed in conical flask on the vibration shaking table, with the 300r/min lh that shakes.
The appearance liquid after the 0.5mL jolting is got in
, or does the appearance liquid after the suitable dilution with PBs.With agar tilt-pour process inoculation plate, carry out colony counting.
are established contrast print group simultaneously and are not added the print group; The contrast print of contrast print group is with onesize but do not contain antimicrobial component by coupons, and other operation sequences do not add the print group and get respectively in 5mL bacteria suspension and 250ml conical flask of 70mL PBS adding all with identical by the coupons group; Mixing; Behind 0 time and vibration lh, respectively get the mixed liquor of 0.5 ware L bacteria suspension and PBS and do suitable dilution, carry out colony counting then.
Test repetition 3 times, calculate bacteriostasis rate by formula (c3):
X5=(A-B)/A100%…………………………………………………(C2)
X5---bacteriostasis rate, %
A---contrast is by the preceding average clump count of test agent vibration.
---by the average clump count in test agent vibration back.
Evaluation criterion
The clump count that does not add the print group is 1 * 10
4
～9 * 10
4
Cfu/mL is together, and average clump count difference is in 10% before and after the sample vibration, and test is effective; By the difference of coupons group bacteriostasis rate with the antibacterial guilt of contrast print; 26%, product has antibacterial action.
The bacteriostatic test result
2.5 conclusion
Has stronger antibacterial and bacteriostasis
3, skin allergic reaction test and acute skin irritation test
3.1 test method
skin irritatin test and skin allergic reaction test method are undertaken by corresponding test method in " disinfectant toxicological experiment technology " in Ministry of Public Health's " disinfection technology standard " (third edition) first fascicle " experimental technique standard " (1999).
(1) blank that is used for skin irritatin test should be: physiological saline and patch paper
In skin allergic reaction, sensitization is handled and is excited the used dosage of processing to be consistent
(2).
Sample preparation
The product of a patch paper size is cut in
with cross-section mode.For the product of doing, like diaper, gynecologic menstrual amenities, be attached on the skin after wetting with physiological saline, covering with patch paper.Wet product, like wet towel, then the suitable area of cutting on request directly is attached on the skin, covers with patch paper again.
Criterion
with the appropriate section of Ministry of Public Health's " disinfection technology standard " (third edition) first fascicle " experimental technique standard " (1999) " toxicology test result's final decision " as the result of the test decision principle.
Result of the test
3.4.1 guinea pig skin allergy (sensitization) result of the test
3.4.2 skin irritatin reaction integrated value table
3.5 conclusion
This is tried thing sensitization rate (%)=0, according to sensitization strength grading standard, belongs to and does not see skin allergic reaction;
are tried thing rabbit acute skin irritation intensity are belonged to nonirritant.
One, to the test of embodiment 3 and 4
embodiment 3 and 4 is mainly used in wet sanitary napkins and sanitary wet towel for baby, and the target customer is the high-end consumer group.The concrete test as follows:
1. microbial contamination detects (detection method is with embodiment 1 and 2)
1.1 microbial contamination testing result
| Detect index | Standard value | Measured value |
| Total number of bacterial colonies (cfg/g) | ≤20 | ＜1 |
| Fungi | Must not detect | Do not detect |
| Coliform | Must not detect | Do not detect |
| Pseudomonas aeruginosa | Must not detect | Do not detect |
| Staphylococcus aureus | Must not detect | Do not detect |
| Hemolytic streptococcus | Must not detect | Do not detect |
1.2 conclusion
The microbial contamination testing result of
this wet towel meets the regulation of " disposable use amenities sanitary standard " GB15979-2002.
The bacteria quantified BA
2.1 sample is collected
have good representativeness for sample is had, and from each embodiment packing, randomly draw 20 minimum marketing packing samples at least, and wherein 5 keep sample, and do antibacterial or the bactericidal property test, do stability test for 10 for 5.
Test organisms and the preparation of bacterium liquid
2.2.1 test organisms
2.2.1.1 bacterium: staphylococcus aureus (ATCC 6538), Escherichia coli (8099 or ATCC 25922), pseudomonas aeruginosa (ATCC15422)
2.2.1.2 saccharomycete: Candida albicans (ATCC 10231).
bacterium liquid preparation: get the bacterial strain nutrient agar inclined-plane fresh cultured thing (18～24h) in the 3rd～14 generation; (hereinafter to be referred as PBS) washes lawn with the 5ml0.03mol/L phosphate buffer, and the back is diluted to desired concn with above-mentioned PBS to make bacterium suspend evenly.
The BA step
wash test organisms 24h slant culture with PBS, (require concentration to be: drip on the contrast print with 100 μ L, reclaiming the bacterium number is 1 * 104～9 * 104cfu/ sheet to process bacteria suspension.
get that (each 4 of 2.0cm * 3.0cm) and contrast prints (with the sample homogeneous material, equal size, but do not contain anti-biotic material, and through sterilization treatment) are divided into 4 groups and place in 4 sterilization plates by coupons.
Above-mentioned bacteria suspension is got in
, print on is dripped 100 μ Ls, evenly coating by coupons with contrasting at each respectively; Pick up counting; Effect 1,2,3mi n. contain the corresponding nertralizer of 5mL in vitro with the print input respectively with aseptic tweezer, and fully mixing is done suitably dilution; Get wherein 2～3 dilution factors then, draw 0.5mI respectively., place two plates, with cool nutrient agar (bacterium) or sabouraud's agar (saccharomycete) 15ml to 40～50 ℃.Pour into, rotate plate, make it full and uniform, agar solidifies the back upset cultivates 48h (bacterium) or 72h (saccharomycete) for dull and stereotyped .35 ℃ ± 2 ℃, makes viable bacteria bacterium counting.
Test repetition 3 times, calculate sterilizing rate by formula (C1):
In
formula: X.---sterilizing rate, %:
A---the average clump count of control sample;
B---by the average clump count of test agent.
.2.2 estimating mark pushes away
sterilizing rate>=90%, product has bactericidal action.
To the test organisms sterilization effect
Annotate
: the equal asepsis growth of negative control.
Conclusion
Under 20 ℃ ± 1 ℃ test temperature, embodiment 3 and 4 wet towel effects 1 minute, 2 minutes, 3 minutes
To staphylococcus aureus, Escherichia coli,Pseudomonas aeruginosa and Candida albicans
Kill logarithm value all＞5.00.
Skin allergic reaction test and acute skin irritation test (test method is with embodiment 1 and 2)
3.1 guinea pig skin allergy (sensitization) result of the test
3.2 skin irritatin reaction integrated value table
3.3 conclusion
This is tried thing sensitization rate (%)=0
, according to sensitization strength grading standard, belongs to and does not see skin allergic reaction.
Tried thing rabbit acute skin irritation intensity is belonged to nonirritant
Three, to the test of embodiment 5
embodiment 5 masters are used for the sterilization of body surface and the sterilization of toy for children, and the target customer is high-end consumer groups.
1. bacteria quantified BA (test method is with embodiment 3 and 4)
1.1 to the test organisms sterilization effect
Annotate: the equal asepsis growth of negative control.
Conclusion
Under 20 ℃ ± 1 ℃ test temperature, embodiment 3 and 4 wet towel effects 1 minute, 2 minutes, 3 minutes
To staphylococcus aureus, Escherichia coli,Pseudomonas aeruginosa
Kill logarithm value all＞5.00.
To the skin degerming field trial
The skin degerming site test results:
| Catalogue number(Cat.No.) | Control group growth clump count (cfu/cm 2） | Test group growth clump count (cfu/cm 2） | Kill logarithm value |
| 1 | 150 | 1 | ＞1.00 |
| 2 | 740 | 1 | ＞1.00 |
| 3 | 1260 | 3 | ＞1.00 |
| 4 | 10 | 1 | 1.00 |
| 5 | 33 | 1 | ＞1.00 |
| 6 | 27 | 0 | ＞1.00 |
| 7 | 810 | 2 | ＞1.00 |
| 8 | 132 | 2 | ＞1.00 |
| 9 | 142 | 2 | ＞1.00 |
| 10 | 620 | 2 | ＞1.00 |
| 11 | 122 | 22 | 0.75 |
| 12 | 190 | 3 | ＞1.00 |
| 13 | 110 | 4 | ＞1.00 |
| 14 | 150 | 11 | ＞1.00 |
| 15 | 344 | 9 | ＞1.00 |
| 16 | 48 | 2 | ＞1.00 |
| 17 | 39 | 3 | ＞1.00 |
| 18 | 46 | 1 | ＞1.00 |
| 19 | 94 | 1 | ＞1.00 |
| 20 | 152 | 2 | ＞1.00 |
| 21 | 124 | 1 | ＞1.00 |
| 22 | 150 | 3 | ＞1.00 |
| 23 | 51 | 13 | 0.60 |
| 24 | 23 | 4 | 0.76 |
| 25 | 22 | 1 | ＞1.00 |
| 26 | 7 | 1 | 0.85 |
| 27 | 12 | 1 | ＞1.00 |
| 28 | 10 | 2 | 0.70 |
| 29 | 13 | 1 | ＞1.00 |
| 30 | 114 | 1 | ＞1.00 |
| On average | 192 | 4 | ＞1.00 |
2.4 conclusion
sample is to the inboard stage casing of upper arm skin wiping sterilization 2 minutes, and natural bacteria on the skin on average killed logarithm value＞1.00.
The surface disinfection field trial
The surface disinfection site test results:
| Catalogue number(Cat.No.) | Control group growth clump count (cfu/cm 2） | Test group growth clump count (cfu/cm 2） | Kill logarithm value |
| 1 | 14 | 1 | ＞1.00 |
| 2 | 9 | 0 | 0.95 |
| 3 | 15 | 0 | ＞1.00 |
| 4 | 16 | 0 | 1.00 |
| 5 | 13 | 0 | ＞1.00 |
| 6 | 20 | 0 | ＞1.00 |
| 7 | 15 | 0 | ＞1.00 |
| 8 | 13 | 0 | ＞1.00 |
| 9 | 11 | 0 | ＞1.00 |
| 10 | 15 | 0 | ＞1.00 |
| 11 | 19 | 0 | ＞1.00 |
| 12 | 17 | 0 | ＞1.00 |
| 13 | 14 | 0 | ＞1.00 |
| 14 | 14 | 0 | ＞1.00 |
| 15 | 16 | 0 | ＞1.00 |
| 16 | 22 | 0 | ＞1.00 |
| 17 | 22 | 0 | ＞1.00 |
| 18 | 13 | 0 | ＞1.00 |
| 19 | 17 | 0 | ＞1.00 |
| 20 | 19 | 0 | ＞1.00 |
| 21 | 25 | 0 | ＞1.00 |
| 22 | 20 | 0 | ＞1.00 |
| 23 | 23 | 0 | ＞1.00 |
| 24 | 20 | 0 | ＞1.00 |
| 25 | 18 | 0 | ＞1.00 |
| 26 | 13 | 0 | ＞1.00 |
| 27 | 15 | 0 | ＞1.00 |
| 28 | 17 | 0 | ＞1.00 |
| 29 | 20 | 0 | ＞1.00 |
| 30 | 14 | 0 | ＞1.00 |
| On average | 17 | 1 | ＞1.00 |
3.4 conclusion
sample is to wooden painted surface wiping sterilization 2 minutes, and its surperficial natural bacteria on average killed logarithm value＞1.00.
Claims (3)
1. multifunctional wet towel that contains orange essence is characterized in that orange essence and auxiliary material poured in the RO pure water and mixes, and stirs, and is static, then the gained mixed liquor evenly is sprayed on the spunlace non-woven cloth, packs and promptly get the towel that wets; Mixture formula is following:
Component content (weight %)
Orange essence 0.05-5%;
Auxiliary material 0-10%;
RO pure water 85-99.95%.
2. the multifunctional wet towel that contains orange essence according to claim 1; It is characterized in that described orange essence is the product that from oranges and tangerines, extracts, main component is oranges and tangerines fruit elite, vitamin C, citric acid, amino acid, citrus pectin, glyceride and glycerine.
3. the multifunctional wet towel that contains orange essence according to claim 1 is characterized in that described auxiliary material is natural materials such as natural glycerin, hyaluronic acid, pyrrolidone sodium carboxylate, aloe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102870246A CN102450973A (en) | 2011-09-26 | 2011-09-26 | Multifunctional wet tissue containing orange essence |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102870246A CN102450973A (en) | 2011-09-26 | 2011-09-26 | Multifunctional wet tissue containing orange essence |
Publications (1)
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| CN2011102870246A Pending CN102450973A (en) | 2011-09-26 | 2011-09-26 | Multifunctional wet tissue containing orange essence |
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| CN (1) | CN102450973A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340804A (en) * | 2013-07-17 | 2013-10-09 | 深圳市妍倩科技有限公司 | Special wet tissue for cleaning and disinfecting nipples of female in lactation period |
| CN104523477A (en) * | 2014-12-08 | 2015-04-22 | 成都顺发消洗科技有限公司 | Preparation method for disinfecting wet wipe |
| CN105662895A (en) * | 2016-02-24 | 2016-06-15 | 浙江绿飞诗日用品有限公司 | Water-based wet tissue |
| CN107737200A (en) * | 2017-10-19 | 2018-02-27 | 黑龙江隆浩生物科技股份有限公司 | A kind of citrus antibacterial liquid and preparation method thereof |
| CN108812713A (en) * | 2018-06-04 | 2018-11-16 | 上海克拉方今环保科技有限公司 | A kind of antibacterial wet tissue and its preparation method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887355A (en) * | 2006-07-27 | 2007-01-03 | 怀星 | New use of orange essence in sterilizing |
| CN101703071A (en) * | 2009-11-13 | 2010-05-12 | 北京欧凯纳斯科技有限公司 | Disinfectant composition containing molecular-state chlorine dioxide and applications thereof |
| WO2010139365A1 (en) * | 2008-06-04 | 2010-12-09 | Edmak Limited | Disinfectant comprising electrochemically activated water and grapefruit seed extract |
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2011
- 2011-09-26 CN CN2011102870246A patent/CN102450973A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887355A (en) * | 2006-07-27 | 2007-01-03 | 怀星 | New use of orange essence in sterilizing |
| WO2010139365A1 (en) * | 2008-06-04 | 2010-12-09 | Edmak Limited | Disinfectant comprising electrochemically activated water and grapefruit seed extract |
| CN101703071A (en) * | 2009-11-13 | 2010-05-12 | 北京欧凯纳斯科技有限公司 | Disinfectant composition containing molecular-state chlorine dioxide and applications thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340804A (en) * | 2013-07-17 | 2013-10-09 | 深圳市妍倩科技有限公司 | Special wet tissue for cleaning and disinfecting nipples of female in lactation period |
| CN104523477A (en) * | 2014-12-08 | 2015-04-22 | 成都顺发消洗科技有限公司 | Preparation method for disinfecting wet wipe |
| CN105662895A (en) * | 2016-02-24 | 2016-06-15 | 浙江绿飞诗日用品有限公司 | Water-based wet tissue |
| CN107737200A (en) * | 2017-10-19 | 2018-02-27 | 黑龙江隆浩生物科技股份有限公司 | A kind of citrus antibacterial liquid and preparation method thereof |
| CN108812713A (en) * | 2018-06-04 | 2018-11-16 | 上海克拉方今环保科技有限公司 | A kind of antibacterial wet tissue and its preparation method and application |
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