CN102428870A - Preparation method for artificial seeds of dendrobium candidum - Google Patents
Preparation method for artificial seeds of dendrobium candidum Download PDFInfo
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- CN102428870A CN102428870A CN2011102844932A CN201110284493A CN102428870A CN 102428870 A CN102428870 A CN 102428870A CN 2011102844932 A CN2011102844932 A CN 2011102844932A CN 201110284493 A CN201110284493 A CN 201110284493A CN 102428870 A CN102428870 A CN 102428870A
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Abstract
The invention discloses a preparation method for artificial seeds of dendrobium candidum. The method comprises: inducing dendrobium candidum stems in a culture medium so as to obtain protocorms, which are then subjected to proliferation culture, and transferring the protocorms into an MS culture solution with 0.5mg/L of ABA (abscisic acid) for shaking culture, changing the culture solution every 10d, after 30d, selecting a protocorm as an embedding propagule, making artificial seeds by an instillation method, taking the MS culture medium, 4mg/L of 6-BA, 0.4mg/L of NAA, 2% of sodium alginate and 0.2% of carbendazim to prepare basic artificial endosperm, adding 2% of chitosan and 5.0-10% of nanometer TiO2 into sodium alginate so as to form an artificial seed coat matrix, and carrying out research on germination and storage of artificial seeds. The preparation method of the invention can enhance seed germination rate and make the seeds convenient for storage and transportation. Adding of a special material into artificial endosperm or an artificial seed coat in the invention can promote germination of the artificial seeds or prolong storage of the artificial seeds.
Description
Technical field
The present invention relates to a kind of preparation method of dendrobium candidum artificial seed, is a kind of plant efficient fast breeding technique, belongs to biological technical field.
Background technology
Dendrobium candidum (Dendrobium candidum) claim ribbed hedyotis herb again, is a kind of medicinal plant of preciousness, is considered to the treasure of enriching yin help since ancient times, has beneficial stomach and give birth to effects such as Feng, nourishing Yin and clearing heat, the sharp throat that makes eye bright.Shennong's Herbal as far back as Qin Han period is just claimed " dendrobium candidum master hinder in, remove that numbness, the therapeutic method to keep the adverse qi flowing downward, tonifying five zang organs consumptive disease are thin and weak, reinforcing yin essence, obey thick large intestines for a long time "; In Compendium of Material Medica, Li Shizhen (1518-1593 A.D.) thinks that the stem of noble dendrobium is " herbal classic is top grade, and is sweet, light, little salty, cures mainly in the wound, removes the numbness therapeutic method to keep the adverse qi flowing downward, never sufficient in the benefit, flat stomach Qi, longue meat, intelligence development removes frightened, makes light of one's life by commiting suicide and prolongs life ".Dendrobium candidum is Chinese distinctive rare medicinal herbs, and quilts such as a direct sum glossy ganoderma, genseng, Cordyceps sinensis are classified top grade Chinese medicine as since thousand, and is described as first of the Chinese nine immortal grass by Taoist school health classical " Taoist Scriptures ".Among the peoplely then be called " the celestial grass of help ", because of it mainly is distributed on the trunk or rock in the mountain valley half dark and damp woods of height above sea level 600-2500m, wild resource has been on the verge of disappearance, is classified as rare wild medicinal plant by State Council and protects in 1987.
The method that the artificial breeding dendrobium candidum generally uses now is the method for plant tissue culture; Utilize tissue culture technique can solve fast numerous problem to a certain extent; But its test-tube plantlet production cycle is long, and cost is high, and transplanting also is difficult for into alive; And inconvenience is stored and transportation, thereby has limited the scale property production of dendrobium candidum.
The seed of dendrobium candidum does not have endosperm, so germination rate is very low under the natural conditions.It is low or be difficult to promote fine germplasm resources fast with seminal propagation, crop that fertility is not good enough that artificial seed can be bred ripening rate fast, and this will advance to automation direction plant husbandry production, create favorable conditions.Artificial seed (artificial seeds) is the meristematic tissue (bulb, bud, callus, embryoid etc.) that the somatic embryo that the plant stereoscopic culture produces maybe can develop into whole plant to be embedded in form in the shell that contains nutrient component and defencive function, granule that can germination and emergence under the condition that suits.
Advantages such as the factorial seedling growth that develops into dendrobium candidum of artificial seed technology provides a new approach, and the manufacturing process of artificial seed is organized the cultivation seedling relatively has less investment, has reduced transplanting domestication process, with short production cycle.Modern study has been done a lot of explorations at aspects such as artificial endosperm, embedding method, people's work post skin, storage methods, but still has a lot of problems not solve for germination rate and the planting percent and the storage condition etc. of artificial seed.The selection of embedding medium and manufacture craft need further to improve, and should improve the germination rate of seed, convenient again storage transportation.In artificial endosperm or people's work post skin, adding special material has become the emphasis of people's research with the storage problem that promotes its sprouting or prolongation artificial seed.
Summary of the invention
We aim to provide a kind of preparation method of dendrobium candidum artificial seed the present invention, and solving the problems referred to above of prior art, but dendrobium candidum is bred in this method mechanized operation, convenient and swift, low cost.
The object of the invention can solve through following technical scheme.
A kind of preparation method of dendrobium candidum artificial seed, its concrete steps are:
(1) protocorm induces
Select vigorous, the disease-free dendrobium candidum healthy plant of growth; Per 2 joints are one section beaker of putting into 500ml; Add a small amount of washing powder, prick rim of a cup, tighten with the engineering line then with gauze individual layer canopy; Be placed on flushing 30min under the water tap, amount of flush water is adjusted to can make among the dendrobium candidum stem Duan Zaishui and is floated to good.Main effect is flush away appearance dust and bulky grain bacterium colony, mycelia etc.Material after the above-mentioned completion is taken on the desinfection chamber superclean bench; Carry out the secondary sterilization sterilization: the bract of stem segment with axillary buds is dialled; Alcohol speed with 70% is soaked 25s, soaks 10min with 0.1% mercuric chloride solution then, constantly shakes beaker in the sterilization process and makes sterilization more thorough; Use aseptic water washing then 5 times, it is residual to remove surface drug.
It is one section that above-mentioned material is cut into per 1 joint, removes the stem section part that medicine soaks into, and is inoculated in the ready-made in advance differentiation and proliferation medium tissue culture bottle, is placed on the medium and cultivates.Condition of culture: 26 ℃ of temperature, light application time 12h, intensity of illumination 2000Lx induces protocorm behind the 30d., described differentiation and proliferation medium is the mixing of MS medium, 4mg/L 6-BA (6-benzyl aminopurine) and 0.4mg/L NAA (methyl).
(2) making of dendrobium candidum artificial seed
Change protocorm in the MS culture fluid that contains 0.5mg/L ABA (S-abscisic acid) concussion cultivation (100r/min), every 10d changes 1 culture fluid, chooses length behind the 30d and takes advantage of the wide 0.5-1.5mm of being about * 2.0-3.4mm protocorm as the embedding brood body.NAA (methyl)+2% sodium alginate+0.2% carbendazim with 6-BA (6-benzyl aminopurine)+0.4mg/L of MS+4mg/L is basic artificial endosperm;, maintenance moisture ventilative in order to increase is convenient to absorb, and adds the nano-TiO of starch, 1.0-2.0% active carbon, 1.0-2.0% water-loss reducer and the 1.0-3.0% of 1.5-2.0% on this basis
2, with the shitosan of interpolation 2% in the sodium alginate and the nano-TiO of 5.0%-10%
2Behaviour work post scytoblastema matter.
On superclean bench, with tweezers protocorm is immersed in the embedding liquid (being artificial endosperm) that NAA, 2% sodium alginate and 0.2% carbendazim of 6-BA with MS medium, 4mg/L, 0.4mg/L be made into, stirring with glass bar fully mixes protocorm and embedding medium.The semigel state sodium alginate that will be surrounded by protocorm with dropper behind the 5-10min splashes into 2% CaCl of people's work post scytoblastema matter
2Be cured in the solution, control every and comprise a protocorm, with culture fluid rinsing 15min, promptly get artificial seed behind the 30min.
More than be mass percent concentration.
On different sprouting matrix, carry out sprouting test, sprouting matrix is respectively the MS medium, plantation matrix is sprouted experiment.Artificial seed 30d germination rate in seed MS medium is 98.0%, and the germination rate in plantation matrix can reach 75.0%, and the germination rate on outdoor seedbed still can reach 70%.
The artificial seed of making is put into the triangular flask of sterilization and oven dry, seal, under 4 ℃, store 30d and 90d respectively with sealing film.
Both can improve the germination rate of seed through preparation method of the present invention, convenient again storage transportation.In artificial endosperm or people's work post skin, add special material and can also promote its sprouting or the storage that prolongs artificial seed.
Embodiment
Further set forth technical characterstic of the present invention below in conjunction with specific embodiment.
(1) protocorm induces
Select vigorous, the disease-free dendrobium candidum healthy plant of growth; Per 2 joints are one section beaker of putting into 500ml; Add a small amount of washing powder, prick rim of a cup, tighten with the engineering line then with gauze individual layer canopy; Be placed on flushing 30min under the water tap, amount of flush water is adjusted to can make among the dendrobium candidum stem Duan Zaishui and is floated to good.Main effect is flush away appearance dust and bulky grain bacterium colony, mycelia etc.Material after the above-mentioned completion is taken on the desinfection chamber superclean bench; Carry out the secondary sterilization sterilization: the bract of stem segment with axillary buds is dialled; Alcohol speed with 70% is soaked 25s, soaks 10min with 0.1% mercuric chloride solution then, constantly shakes beaker in the sterilization process and makes sterilization more thorough; Use aseptic water washing then 5 times, it is residual to remove surface drug.
It is one section that above-mentioned material is cut into per 1 joint, removes the stem section part that medicine soaks into, and is inoculated in the ready-made in advance differentiation and proliferation medium tissue culture bottle, is placed on the medium and cultivates.Condition of culture: 26 ℃ of temperature, light application time 12h, intensity of illumination 2000Lx induces protocorm behind the 30d., described differentiation and proliferation medium is the mixing of MS medium, 4mg/L 6-BA (6-benzyl aminopurine) and 0.4mg/L NAA (methyl).
(2) making of dendrobium candidum artificial seed
Change protocorm in the MS culture fluid that contains 0.5mg/L ABA concussion cultivation (100r/min), every 10d changes 1 culture fluid, chooses length behind the 30d and takes advantage of the wide 0.5-1.5mm of being about * 2.0-3.4mm protocorm as the embedding brood body.NAA (methyl)+2% sodium alginate+0.2% carbendazim with 6-BA (6-benzyl aminopurine)+0.4mg/L of MS+4mg/L is basic artificial endosperm;, maintenance moisture ventilative in order to increase is convenient to absorb, and adds the nano-TiO of starch, 1.0-2.0% active carbon, 1.0-2.0% water-loss reducer and the 1.0-3.0% of 1.5-2.0% on this basis
2, with the shitosan of interpolation 2% in the sodium alginate and the nano-TiO of 5.0%-10%
2Behaviour work post scytoblastema matter.
On superclean bench, with tweezers protocorm is immersed in the embedding liquid (being artificial endosperm) that NAA, 2% sodium alginate and 0.2% carbendazim of 6-BA with MS medium, 4mg/L, 0.4mg/L be made into, stirring with glass bar fully mixes protocorm and embedding medium.The semigel state sodium alginate that will be surrounded by protocorm with dropper behind the 5-10min splashes into 2% CaCl of people's work post scytoblastema matter
2Be cured in the solution, control every and comprise a protocorm, with culture fluid rinsing 15min, promptly get artificial seed behind the 30min.
The sprouting experiment of artificial seed:
On different sprouting matrix, carry out sprouting test, sprout matrix and be respectively MS medium, plantation matrix, seedbed and sprout experiment.Artificial seed 30d germination rate in seed MS medium is 98.0%, and the germination rate in plantation matrix can reach 75.0%, and the germination rate on outdoor seedbed still can reach 70%.
Dendrobium candidum seed germination rate under field conditions (factors) is merely 5%, and the germination rate of the artificial seed on the market also is merely 80% now, and our product has improved the germination rate of dendrobium candidum artificial seed greatly.
The storage experiment of artificial seed:
The artificial seed of making is put into the triangular flask of sterilization and oven dry, seal, under 4 ℃, store 30d and 90d respectively with sealing film.
With its germination rate on the MS medium behind the artificial seed storage 30d that makes is 95.0%, and germination rate still can reach 90.0% behind the 60d, and planting percent can reach 85.0%.
Now also there is very big problem in the storage for artificial seed on the market, and along with the germination rate of the prolongation artificial seed in storage time can significantly decrease, we still can keep very high germination rate by prepared artificial seed after storage.
Though the report that domestic relevant dendrobium candidum artificial seed is made, only on medium, sprouting successfully of the artificial seed of making is unrealized in the sprouting of planting on matrix and the outdoor seedbed.Artificial endosperm and people's work post skin that the present invention utilizes modern nano material to make; Not only can improve gas permeability, moisture capacity and the water imbibition of sodium alginate to embed system; Shorten the sprout time of artificial seed, and can suppress breeding and the growth of microorganism, reach antibacterial, corrosion-resistant effect; And promote the protocorm in the artificial seed micelle to grow; Thereby improve germination rate and the planting percent of artificial seed on plantation matrix and outdoor seedbed,, thereby open up a new way for breeding of dendrobium candidum for dendrobium candidum artificial seed practicability provides technical support.
Claims (5)
1. the preparation method of a dendrobium candidum artificial seed is characterized in that:
Its concrete steps are:
1) inducing of protocorm: select the dendrobium candidum healthy plant, after segment, sterilization, cleaning, be inoculated in the tissue culture bottle of ready-made in advance differentiation and proliferation medium, on medium, induce protocorm, carry out the enrichment culture of protocorm then;
2) making of artificial seed: protocorm is changed in the MS culture fluid of the ABA that contains 0.5mg/L again, cultivate with the speed concussion of 100r/min, every 10d changes 1 culture fluid; The protocorm of choosing long * wide 0.5-1.5mm * 2.0-3.4mm of being behind the 30d adopts drip to carry out artificial seed and makes as the embedding brood body; With the NAA of the 6-BA of MS medium, 4mg/L, 0.4mg/L, 2% sodium alginate and 0.2% carbendazim is basic artificial endosperm; Add 2% shitosan and 5.0-10% nano-TiO with sodium alginate
2Behaviour work post scytoblastema matter; Protocorm is immersed in the artificial endosperm, protocorm and embedding medium are fully mixed; The semigel state sodium alginate that will be surrounded by protocorm with dropper behind the 5-10min splashes into the 2%CaCl of people's work post scytoblastema matter
2Be cured in the solution, control every and comprise a protocorm, with culture fluid rinsing 15min, promptly get artificial seed behind the 30min.
2. the preparation method of a kind of dendrobium candidum artificial seed according to claim 1 is characterized in that: described differentiation and proliferation medium is: the 6-BA of MS medium, 4mg/L and the NAA of 0.4mg/L mix.
3. the preparation method of a kind of dendrobium candidum artificial seed according to claim 1 is characterized in that:
In the step 1); Described segment, sterilization and cleaning are specially: select vigorous, the disease-free dendrobium candidum healthy plant of growth, per 2 joints are one section beaker of putting into 500ml, add a small amount of washing powder; Prick rim of a cup with gauze individual layer canopy; Tighten with the engineering line then, be placed on flushing 30min under the water tap, amount of flush water is adjusted to can make among the dendrobium candidum stem Duan Zaishui and is floated; Flush away appearance dust and bulky grain bacterium colony, mycelia etc.; Material after the above-mentioned completion is taken on the desinfection chamber superclean bench; Carry out the secondary sterilization sterilization: the bract of stem segment with axillary buds is dialled; Alcohol speed with 70% is soaked 25s, soaks 10min with 0.1% mercuric chloride solution then, constantly shakes beaker in the sterilization process and makes sterilization more thorough; Use aseptic water washing then 5 times, it is residual to remove surface drug.
4. the preparation method of a kind of dendrobium candidum artificial seed according to claim 1 is characterized in that:
In the step 1); Described inoculation, the process of inducing are specially: will be cut into each joint through the stem of noble dendrobium stem section after segment, sterilization and the cleaning is one section; Remove the stem section part that medicine soaks into, be inoculated in the tissue culture bottle of ready-made in advance differentiation and proliferation medium, be placed on the medium and cultivate; Condition of culture: 26 ℃ of temperature, light application time 12h, intensity of illumination 2000Lx; Induce protocorm behind the 30d.
5. the preparation method of a kind of dendrobium candidum artificial seed according to claim 1; It is characterized in that:, maintenance moisture ventilative in order to increase is convenient to absorb, and in artificial endosperm, adds the nano-TiO of starch, 1.0-2.0% active carbon, 1.0-2.0% water-loss reducer and the 1.0-3.0% of 1.5-2.0%
2
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696579A (en) * | 2012-06-05 | 2012-10-03 | 杭州师范大学 | Encapsulation-vitrification ultra-low temperature preservation method for dendrobium protocorm |
| CN102893865A (en) * | 2012-09-20 | 2013-01-30 | 四川农业大学 | Method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl. |
| CN103229718A (en) * | 2013-04-24 | 2013-08-07 | 浙江韵芝堂生物科技有限公司 | Tissue culture rapid propagation method of Dendrobium officinale |
| CN103229719A (en) * | 2013-04-24 | 2013-08-07 | 浙江韵芝堂生物科技有限公司 | Liquid suspension culture method of Dendrobium officinale |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN101258835A (en) * | 2008-04-23 | 2008-09-10 | 昆明理工大学 | Fast reproducing method for high quality seedling of dendrobium officinale |
| CN101849503A (en) * | 2009-10-26 | 2010-10-06 | 刘宏源 | Method for manufacturing dendrobium officinale artificial seeds |
| CN101878736A (en) * | 2010-06-18 | 2010-11-10 | 沈阳药科大学 | Method for producing dendrobium candicum |
-
2011
- 2011-09-22 CN CN2011102844932A patent/CN102428870A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN101258835A (en) * | 2008-04-23 | 2008-09-10 | 昆明理工大学 | Fast reproducing method for high quality seedling of dendrobium officinale |
| CN101849503A (en) * | 2009-10-26 | 2010-10-06 | 刘宏源 | Method for manufacturing dendrobium officinale artificial seeds |
| CN101878736A (en) * | 2010-06-18 | 2010-11-10 | 沈阳药科大学 | Method for producing dendrobium candicum |
Non-Patent Citations (1)
| Title |
|---|
| 巩振辉等: "《植物组织培养》", 30 August 2007, 化学工业出版社 * |
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| CN102696579A (en) * | 2012-06-05 | 2012-10-03 | 杭州师范大学 | Encapsulation-vitrification ultra-low temperature preservation method for dendrobium protocorm |
| CN102696579B (en) * | 2012-06-05 | 2014-04-09 | 杭州师范大学 | Encapsulation-vitrification ultra-low temperature preservation method for dendrobium protocorm |
| CN102893865A (en) * | 2012-09-20 | 2013-01-30 | 四川农业大学 | Method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl. |
| CN103229719B (en) * | 2013-04-24 | 2016-04-13 | 浙江韵芝堂生物科技有限公司 | A kind of method that dendrobium candidum liquid suspension is cultivated |
| CN103229718A (en) * | 2013-04-24 | 2013-08-07 | 浙江韵芝堂生物科技有限公司 | Tissue culture rapid propagation method of Dendrobium officinale |
| CN103229719A (en) * | 2013-04-24 | 2013-08-07 | 浙江韵芝堂生物科技有限公司 | Liquid suspension culture method of Dendrobium officinale |
| CN103229718B (en) * | 2013-04-24 | 2016-04-13 | 浙江韵芝堂生物科技有限公司 | A kind of tissue culture and rapid propagation method of dendrobium candidum |
| CN103621405A (en) * | 2013-11-27 | 2014-03-12 | 江苏大学 | Artificial corychophramus violaceua seed making method |
| CN103621405B (en) * | 2013-11-27 | 2015-05-13 | 江苏大学 | Artificial corychophramus violaceua seed making method |
| CN104737902A (en) * | 2013-12-25 | 2015-07-01 | 广州中医药大学 | Nanometer artificial seed of Dendrobium officinale and manufacturing method thereof |
| CN104737902B (en) * | 2013-12-25 | 2017-05-03 | 广州中医药大学 | Nanometer artificial seed of Dendrobium officinale and manufacturing method thereof |
| CN105684900A (en) * | 2016-01-22 | 2016-06-22 | 大连中植环境生物科技有限公司 | Preparation method and special culture solution for artificial seeds of broussonetia papyifera |
| CN105684900B (en) * | 2016-01-22 | 2018-05-25 | 大连中植环境生物科技有限公司 | A kind of method and its special culture solution for preparing paper mulberry artificial seed |
| CN105557527A (en) * | 2016-01-26 | 2016-05-11 | 南京农业大学 | Manufacturing method for artificial cymbidium seeds |
| CN105557527B (en) * | 2016-01-26 | 2018-06-12 | 南京农业大学 | A kind of hybrid cymbidium artificial seed production method |
| CN105557528A (en) * | 2016-01-28 | 2016-05-11 | 江苏大学 | Preparation method of antibacterial artificial seeds of orychophragmus violaceus |
| CN107896991A (en) * | 2017-11-27 | 2018-04-13 | 中国热带农业科学院热带生物技术研究所 | A kind of Jujun grasses artificial seed production method |
| CN113475189A (en) * | 2021-05-27 | 2021-10-08 | 云南大学 | Orchid plant seed-fungus symbiotic germination complex and preparation method and application thereof |
| CN113950889A (en) * | 2021-10-19 | 2022-01-21 | 绍兴文理学院元培学院 | Making method of artificial seeds of curcuma alismatifolia |
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Application publication date: 20120502 |