CN102428172A - 多潜能和多能细胞在微载体上的培养 - Google Patents

多潜能和多能细胞在微载体上的培养 Download PDF

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CN102428172A
CN102428172A CN2010800219292A CN201080021929A CN102428172A CN 102428172 A CN102428172 A CN 102428172A CN 2010800219292 A CN2010800219292 A CN 2010800219292A CN 201080021929 A CN201080021929 A CN 201080021929A CN 102428172 A CN102428172 A CN 102428172A
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cells
microcarrier
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史蒂文·侯
陈冠良
安德烈·朔
肖尔·鲁维尼
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

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  • Genetics & Genomics (AREA)
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  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
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CN2010800219292A 2009-03-20 2010-03-12 多潜能和多能细胞在微载体上的培养 Pending CN102428172A (zh)

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US16178609P 2009-03-20 2009-03-20
US61/161,786 2009-03-20
PCT/SG2010/000091 WO2010107392A1 (en) 2009-03-20 2010-03-12 Culture of pluripotent and multipotent cells on microcarriers

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US (1) US9150829B2 (https=)
EP (1) EP2408903B1 (https=)
JP (1) JP2012520672A (https=)
KR (1) KR20110137805A (https=)
CN (1) CN102428172A (https=)
SG (1) SG174471A1 (https=)
WO (1) WO2010107392A1 (https=)

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CN103031270A (zh) * 2013-01-05 2013-04-10 绍兴文理学院 胆管上皮细胞的高效扩增和培养方法
CN103305453A (zh) * 2013-05-23 2013-09-18 侯亚义 一种脐带间充质干细胞的微载体培养系统
CN105683363A (zh) * 2013-04-16 2016-06-15 马斯特里赫特大学 类胚体，基于细胞系的人工囊胚
CN108753678A (zh) * 2012-07-24 2018-11-06 日产化学工业株式会社 培养基组合物以及使用所述组合物培养细胞或组织的方法
CN110452869A (zh) * 2016-11-26 2019-11-15 中国科学院大连化学物理研究所 一种高通量形成拟胚体的微阵列芯片的制备方法与应用
CN112843338A (zh) * 2021-02-08 2021-05-28 康膝生物医疗(深圳)有限公司 一种制备含胶原蛋白的间充质干细胞注射剂的方法
CN113201488A (zh) * 2021-05-13 2021-08-03 圣美基因科技(北京)有限公司 一种间充质干细胞大规模培养方法
CN113388582A (zh) * 2021-06-21 2021-09-14 香港再生医学有限公司 促使iPSC分化为外周神经干细胞的方法及培养基和系统
CN113631699A (zh) * 2018-10-24 2021-11-09 赫贝细胞股份有限公司 用于生产造血谱系细胞的方法和系统
CN114929856A (zh) * 2019-10-30 2022-08-19 总医院公司 Ipsc衍生的肺泡细胞的高通量培养
CN115747197A (zh) * 2023-01-06 2023-03-07 中国肉类食品综合研究中心 一种可食用3d打印生物墨水及其制备方法和在培育肉中的应用
CN115997005A (zh) * 2020-06-11 2023-04-21 迈索布拉斯特国际有限公司 间充质谱系前体或干细胞的3d培养
CN116286610A (zh) * 2021-12-21 2023-06-23 国玺干细胞应用技术股份有限公司 稳定扩增富潜能干细胞的方法
WO2023125753A1 (en) * 2021-12-31 2023-07-06 Beijing Theraxyte Bioscience Co. Ltd. Compositions and methods for culturing stem cells

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WO2015195780A1 (en) * 2014-06-17 2015-12-23 Lieber Institute For Brain Development Assay for cell specification in self-renewing human pluripotent stem cells
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CN106282094B (zh) * 2016-10-13 2018-11-27 吴欣怡 人皮肤来源的前体细胞诱导分化为角膜内皮样细胞的方法
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WO2020080561A1 (ko) * 2018-10-15 2020-04-23 (주)메디톡스 만능성 줄기세포 배양용 배지 및 그를 이용한 배양 방법
KR102120378B1 (ko) * 2019-07-04 2020-06-08 연세대학교 산학협력단 암 오가노이드의 제조 방법 및 이의 용도
WO2021162206A1 (ko) * 2020-02-10 2021-08-19 주식회사 마이크로바이오틱스 모낭조직 배양액을 포함하는 탈모의 예방 또는 치료용 조성물
KR102458606B1 (ko) * 2020-03-06 2022-10-25 경희대학교 산학협력단 마이크로캐리어 기반 4차원 세포 배양기 및 이를 이용한 세포배양 모니터링 방법
WO2021193596A1 (ja) * 2020-03-24 2021-09-30 学校法人自治医科大学 一時処理培地、処理キット、胚発生停止抑制剤、胚発生停止抑制方法、発生工学産物作製方法、移植方法、治療方法、及び発生工学産物
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Cited By (16)

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Publication number Priority date Publication date Assignee Title
CN108753678A (zh) * 2012-07-24 2018-11-06 日产化学工业株式会社 培养基组合物以及使用所述组合物培养细胞或组织的方法
CN108753679A (zh) * 2012-07-24 2018-11-06 日产化学工业株式会社 培养基组合物以及使用所述组合物培养细胞或组织的方法
CN103031270A (zh) * 2013-01-05 2013-04-10 绍兴文理学院 胆管上皮细胞的高效扩增和培养方法
CN105683363A (zh) * 2013-04-16 2016-06-15 马斯特里赫特大学 类胚体，基于细胞系的人工囊胚
CN103305453A (zh) * 2013-05-23 2013-09-18 侯亚义 一种脐带间充质干细胞的微载体培养系统
CN110452869A (zh) * 2016-11-26 2019-11-15 中国科学院大连化学物理研究所 一种高通量形成拟胚体的微阵列芯片的制备方法与应用
CN113631699A (zh) * 2018-10-24 2021-11-09 赫贝细胞股份有限公司 用于生产造血谱系细胞的方法和系统
CN114929856A (zh) * 2019-10-30 2022-08-19 总医院公司 Ipsc衍生的肺泡细胞的高通量培养
CN115997005A (zh) * 2020-06-11 2023-04-21 迈索布拉斯特国际有限公司 间充质谱系前体或干细胞的3d培养
CN112843338A (zh) * 2021-02-08 2021-05-28 康膝生物医疗(深圳)有限公司 一种制备含胶原蛋白的间充质干细胞注射剂的方法
CN112843338B (zh) * 2021-02-08 2022-05-20 康膝生物医疗(深圳)有限公司 一种制备含胶原蛋白的间充质干细胞注射剂的方法
CN113201488A (zh) * 2021-05-13 2021-08-03 圣美基因科技(北京)有限公司 一种间充质干细胞大规模培养方法
CN113388582A (zh) * 2021-06-21 2021-09-14 香港再生医学有限公司 促使iPSC分化为外周神经干细胞的方法及培养基和系统
CN116286610A (zh) * 2021-12-21 2023-06-23 国玺干细胞应用技术股份有限公司 稳定扩增富潜能干细胞的方法
WO2023125753A1 (en) * 2021-12-31 2023-07-06 Beijing Theraxyte Bioscience Co. Ltd. Compositions and methods for culturing stem cells
CN115747197A (zh) * 2023-01-06 2023-03-07 中国肉类食品综合研究中心 一种可食用3d打印生物墨水及其制备方法和在培育肉中的应用

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EP2408903A1 (en) 2012-01-25
JP2012520672A (ja) 2012-09-10
US20120009645A1 (en) 2012-01-12
WO2010107392A1 (en) 2010-09-23
KR20110137805A (ko) 2011-12-23
EP2408903A4 (en) 2013-02-20
US9150829B2 (en) 2015-10-06
EP2408903B1 (en) 2014-08-06
SG174471A1 (en) 2011-10-28

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Application publication date: 20120425