CN102422787A - Method for increasing content of secondary metabolite 1-deoxynojirimycin of mulberry tree - Google Patents
Method for increasing content of secondary metabolite 1-deoxynojirimycin of mulberry tree Download PDFInfo
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- CN102422787A CN102422787A CN2011102701943A CN201110270194A CN102422787A CN 102422787 A CN102422787 A CN 102422787A CN 2011102701943 A CN2011102701943 A CN 2011102701943A CN 201110270194 A CN201110270194 A CN 201110270194A CN 102422787 A CN102422787 A CN 102422787A
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Abstract
The invention discloses a method for increasing the content of secondary metabolite 1-deoxynojirimycin of a mulberry tree. According to the method, coronatine is applied to physiological multiphase adjustment of the mulberry tree, so that the secondary metabolism of the mulberry tree is promoted and the yield of the secondary metabolite 1-DNJ (deoxynojirimycin) is increased. Compared with a blank control group, the method can ensure that the content of the 1-DNJ in mulberry leaves is increased by 161 percent when the concentration of the coronatine is 1mumol/L. The coronatine can be used for promoting the increase of the content of the secondary metabolite and is conductive to promoting the production and income increase as well as development and utilization of pharmaceutical/food resources.
Description
Technical field
The invention belongs to biological technical field, relate to the plant growth substance application, particularly a kind of through using plant growth substance to improve the method for mulberry tree 1-DNJ and yield of flavone.
Background technology
(1-Deoxynojirimycin 1-DNJ) has significant hypoglycemic activity for the alkaloid of representative with the 1-DNJ in the mulberry tree (Morus albaL.).1-DNJ is a unexistent alkaloid in a kind of other animal and plant; Content in the mulberry leaf is about 0.11%; The content of Sang Genzhong is 0.14%; Its major function is to suppress the decomposition of invertase, maltose, alpha-glucosaccharase sugar and AMS, thereby reaches the effect that prevents and treats diabetes, has very important academic significance and practical value for the new drug of developing treating diabetes and exploitation mulberry leaf Related product.
From mulberry tree, separate the 1-DNJ with very high medical value and health care value, be the focus of domestic and international research always.Japan scholar Asano isolates multiple polyhydroxylated alkaloid from mulberry tree, comprise 1-DNJ, N-methyl isophthalic acid-DNJ, 2-O-α-D-galactose pyranoside-1-DNJ etc.; People such as Yang Ru are mulberry tree tissue (comprising silkworm excrement) basically with material source; Proposed to extract in a kind of natural products the method (number of patent application: 200910167602) that separates high-purity 1-DNJ; From natural products; Comprise mulberry leaf, ramulus mori, the root bark of white mulberry, mulberry fruit, silkworm excrement, extract separation purity and be higher than 98% 1-DNJ; People such as Ou Yangzhen have proposed a kind ofly from mulberry leaf total alkaloid, to separate the 1-DNJ monomer methods (number of patent application: 200810122998), from mulberry leaf total alkaloid, separate the 1-DNJ monomer, purity is higher than 95%, and yield is greater than 75%; Former love is red to wait the people to disclose the application (number of patent application: 200510026787) of 1-DNJ in preparation treatment diabetic nephropathy drugs; And confirm that first alkaloid 1-DNJ can suppress high sugar and cultivate mesangial cell propagation; And can suppress high sugar cultivation mesangial cell generation extracellular matrix and increase, proved that from a new visual angle glycosidase inhibitor has preventive and therapeutic effect to diabetic nephropathy; Only there is people such as Chen Yan to propose to utilize dayflower as the raw material (number of patent application: 200910027229) that extracts 1-DNJ.Yet 1-DNJ is owing to be the secondary metabolite of plant, and the content in the mulberry tree tissue is lower, causes the purifying difficulty too high, thereby has reduced its utilization of resources degree.Therefore, manage to improve the 1-DNJ accumulation in the mulberry tree tissue, have important academic significance and social benefit for the development and use of 1-DNJ.
Coronatine (Coronatine, COR, C
18H
25NO
4) be a kind of new and effective biogenic plant growth substance; How to secrete outside born of the same parents by the plant pathogen mutation of pseudomonas syringae genus; Can effectively promote the biosynthesis (as improving the output of Chinese yew secondary metabolism antineoplastic taxol) of Secondary Metabolite Production in Plants; Improve stress resistance of plant, the specific activity jasmonic is high 100～10000 times.So far, the research that utilizes coronatine to regulate the mulberry tree secondary metabolism does not appear in the newspapers.
Summary of the invention
Technical problem:
To above problem, the objective of the invention is based on environment-friendly and green plant growth substance coronatine, regulate the mulberry tree secondary metabolism, a kind of method that improves mulberry tree 1-DNJ content is provided.
Technical scheme:
A kind of method that improves 1-DNJ content in the mulberry tree is to the coronatine of the blade spraying 0.001-1 of mulberry tree seedling μ mol/L.Described mulberry tree plant is Moraceae (Morus alba L.), places sterilization quality uniform quartz sand, with the Hoagland pancebrin as source of nutrition.Blade spraying coronatine to the mulberry tree seedling repeats 2-8 time, 1-5 days at interval.1-DNJ is extracted in Sang Youmiao oven dry after handling, measure its content.
Beneficial effect:
Coronatine provided by the invention is regulated the technology of mulberry tree secondary metabolism, can significantly improve the content of 1-DNJ in the mulberry tree tissue fast and effectively.
1-DNJ is the active ingredient of tool hypoglycemic activity.The present invention is applied to the heterogeneous adjusting of physiology of mulberry tree with coronatine, promotes the mulberry tree secondary metabolism, has improved the output of secondary metabolite 1-DNJ greatly.Compare with the blank group, when coronatine concentration 1 μ mol/L, 1-DNJ has improved 161% in the mulberry leaf.Coronatine promotes the raising of secondary metabolite content, helps to promote the increasing both production and income and the development and use of medicine-food two-purpose resource.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
Deionized water is sprouted mulberry tree seed (Morus alba L.), moves in sterilization quartz sand every basin ten strains, totally ten basins.After two weeks, spray 0.001 μ mol/L coronatine, the two days repeat sprayings in interval, totally five times.Rounding the oven dry of strain mulberry seedling weighs.
Accurately take by weighing Mulberry Leaf 0.1g, add 5mL 0.05mol/L HCl, eddy current 1min, ultrasonic Treatment 40min; The centrifugal 30min of 4000r/min collects supernatant, and sediment is again by repetition extracting of last step 1 time; Merge 2 times supernatant, be settled to 10mL, promptly get sample extracting solution.It is 1.0449mg/L that HPLC detects 1-DNJ content.
Embodiment 2
Deionized water is sprouted mulberry tree seed (Morus alba L.), moves in sterilization quartz sand every basin ten strains, totally ten basins.After two weeks, spray 0.001 μ mol/L coronatine, the two days repeat sprayings in interval, totally five times.Rounding the oven dry of strain mulberry seedling weighs.
Accurately take by weighing Mulberry Leaf 0.1g, add 5mL 0.05mol/L HCl, eddy current 1min, ultrasonic Treatment 40min; The centrifugal 30min of 4000r/min collects supernatant, and sediment is again by repetition extracting of last step 1 time; Merge 2 times supernatant, be settled to 10mL, promptly get sample extracting solution.It is 1.7997mg/L that HPLC detects 1-DNJ content, compares with blank control group and has improved 89%.
Embodiment 3
Deionized water is sprouted mulberry tree seed (Morus alba L.), moves in sterilization quartz sand every basin ten strains, totally ten basins.After two weeks, spray 0.010 μ mol/L coronatine, the two days repeat sprayings in interval, totally five times.Rounding the oven dry of strain mulberry seedling weighs.
Accurately take by weighing Mulberry Leaf 0.1g, add 5mL 0.05mol/L HCl, eddy current 1min, ultrasonic Treatment 40min; The centrifugal 30min of 4000r/min collects supernatant, and sediment is again by repetition extracting of last step 1 time; Merge 2 times supernatant, be settled to 10mL, promptly get sample extracting solution.It is 1.9199mg/L that HPLC detects 1-DNJ content, compares with blank control group and has improved 103%.
Embodiment 4
Deionized water is sprouted mulberry tree seed (Morus alba L.), moves in sterilization quartz sand every basin ten strains, totally ten basins.After two weeks, spray 0.100 μ mol/L coronatine, the two days repeat sprayings in interval, totally five times.Rounding the oven dry of strain mulberry seedling weighs.
Accurately take by weighing Mulberry Leaf 0.1g, add 5mL 0.05mol/L HCl, eddy current 1min, ultrasonic Treatment 40min; The centrifugal 30min of 4000r/min collects supernatant, and sediment is again by repetition extracting of last step 1 time; Merge 2 times supernatant, be settled to 10mL, promptly get sample extracting solution.It is 1.7037mg/L that HPLC detects 1-DNJ content, compares with blank control group and has improved 78%.
Embodiment 5
Deionized water is sprouted mulberry tree seed (Morus alba L.), moves in sterilization quartz sand every basin ten strains, totally ten basins.After two weeks, spray 1.000 μ mol/L coronatines, the two days repeat sprayings in interval, totally five times.Rounding the oven dry of strain mulberry seedling weighs.
Accurately take by weighing Mulberry Leaf 0.1g, add 5mL 0.05mol/L HCl, eddy current 1min, ultrasonic Treatment 40min; The centrifugal 30min of 4000r/min collects supernatant, and sediment is again by repetition extracting of last step 1 time; Merge 2 times supernatant, be settled to 10mL, promptly get sample extracting solution.It is 2.4066mg/L that HPLC detects 1-DNJ content, compares with blank control group and has improved 161%.
Claims (3)
1. a method that improves 1-DNJ content in the mulberry tree is characterized in that the coronatine to the blade spraying 0.001-1 of mulberry tree seedling μ mol/L.
2. the method for 1-DNJ content is characterized in that described mulberry tree plant is Moraceae (Morus alba L.) in the raising mulberry tree according to claim 1.
3. the method for 1-DNJ content is characterized in that the blade spraying coronatine to the mulberry tree seedling in the raising mulberry tree according to claim 1 and 2, repeats 1-5 days at interval 2-8 time.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110270194 CN102422787B (en) | 2011-09-14 | 2011-09-14 | Method for increasing content of secondary metabolite 1-deoxynojirimycin of mulberry tree |
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| CN 201110270194 CN102422787B (en) | 2011-09-14 | 2011-09-14 | Method for increasing content of secondary metabolite 1-deoxynojirimycin of mulberry tree |
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| CN102422787B CN102422787B (en) | 2013-06-05 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102675188A (en) * | 2012-05-21 | 2012-09-19 | 江苏科技大学 | Extraction method of 1-desoxynojirimycin in mulberry leaf |
| CN102875450A (en) * | 2012-10-25 | 2013-01-16 | 上海丝绸集团股份有限公司 | Technological method for extracting 1-deoxynojirimycin from mulberry leaf |
| CN105520152A (en) * | 2014-12-11 | 2016-04-27 | 南京覆盆子酒业开发有限公司 | 1-deoxynojirimycin enriched mulberry leaf preparation method and obtained ultra-fine powder |
| CN108184529A (en) * | 2018-01-25 | 2018-06-22 | 江西省蚕桑茶叶研究所 | It is a kind of to improve dish mulberry tree burgeen yield and the cultural method of quality |
| CN110558143A (en) * | 2019-10-18 | 2019-12-13 | 安徽农业大学 | Cyclocarya paliurus cultivation method for improving content of secondary metabolites in cyclocarya paliurus leaves |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1570083A (en) * | 2004-04-29 | 2005-01-26 | 蔡祝南 | High proportion psendomonas syringae production strain and its fermentation method to produce psendomonas syringae |
| CN1685832A (en) * | 2005-03-21 | 2005-10-26 | 中国农业大学 | Method of raising taxinol content in tazus chinensis |
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2011
- 2011-09-14 CN CN 201110270194 patent/CN102422787B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1570083A (en) * | 2004-04-29 | 2005-01-26 | 蔡祝南 | High proportion psendomonas syringae production strain and its fermentation method to produce psendomonas syringae |
| CN1685832A (en) * | 2005-03-21 | 2005-10-26 | 中国农业大学 | Method of raising taxinol content in tazus chinensis |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102675188A (en) * | 2012-05-21 | 2012-09-19 | 江苏科技大学 | Extraction method of 1-desoxynojirimycin in mulberry leaf |
| CN102675188B (en) * | 2012-05-21 | 2014-10-22 | 江苏科技大学 | Extraction method of 1-desoxynojirimycin in mulberry leaf |
| CN102875450A (en) * | 2012-10-25 | 2013-01-16 | 上海丝绸集团股份有限公司 | Technological method for extracting 1-deoxynojirimycin from mulberry leaf |
| CN105520152A (en) * | 2014-12-11 | 2016-04-27 | 南京覆盆子酒业开发有限公司 | 1-deoxynojirimycin enriched mulberry leaf preparation method and obtained ultra-fine powder |
| CN108184529A (en) * | 2018-01-25 | 2018-06-22 | 江西省蚕桑茶叶研究所 | It is a kind of to improve dish mulberry tree burgeen yield and the cultural method of quality |
| CN108184529B (en) * | 2018-01-25 | 2019-12-03 | 江西省蚕桑茶叶研究所 | A kind of cultural method improving dish mulberry tree burgeen yield and quality |
| CN110558143A (en) * | 2019-10-18 | 2019-12-13 | 安徽农业大学 | Cyclocarya paliurus cultivation method for improving content of secondary metabolites in cyclocarya paliurus leaves |
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| CN102422787B (en) | 2013-06-05 |
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