CN102406088A - High-efficiency enteromorpha additive aquatic feed formula - Google Patents
High-efficiency enteromorpha additive aquatic feed formula Download PDFInfo
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- CN102406088A CN102406088A CN2011103944092A CN201110394409A CN102406088A CN 102406088 A CN102406088 A CN 102406088A CN 2011103944092 A CN2011103944092 A CN 2011103944092A CN 201110394409 A CN201110394409 A CN 201110394409A CN 102406088 A CN102406088 A CN 102406088A
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Abstract
The invention belongs to the technical field of marine fish (mullet) bait research and development, relates to a treatment method and a component proportion of high-efficiency marine algae enteromorpha for baits, and aims at using enteromorpha as a new feed resource for development and application and applying the enteromorpha as a low-input high-output algae feed in the breeding industry. The marine green tide algae enteromorpha serving as a high-efficiency feed additive comprises the following components: 300g of fish meal, 300g of soybean meal, 70g of whole wheat flour, 50g of yeast, 50mL of maize germ oil, 15g of soybean lecithin, 5g of sodium alginate, 1g of cellulase, 50000IU of vitamin A, 4000IU of vitamin D, 100mg of vitamin E, 50mg of vitamin B1, 50mg of vitamin B2, 5mg of vitamin B6, 10mu g of vitamin B12, 150mg of nicotinamide, 50mg of calcium pantothenate, 500mg of choline bitartrate, 500mg of vitamin C, 100mg of iron, 10mg of copper, 5mg of zinc, 10mg of magnesium, 1mg of iodine, 10mg of manganese, 100mg of potassium, 5580mg of calcium hydrophosphate, and 250mg of L-lysiante, wherein the enteromorpha is added according to a gradient. A feeding attraction activity test is carried out by detecting the rate of live weight growth of mullet, a specific growth rate and a bait coefficient, and the best feed enteromorpha gradient is finally determined. According to the invention, the enteromorpha serving as a low-cost high-efficiency feed additive can be quickly applied in the breeding industry.
Description
Technical field
Marine fishes (mullet) bait research and development technical field under the present invention.The present invention relates to bait with efficient marine algae Enteromorpha processing method and composition proportion.The present invention is intended to Enteromorpha is developed and applied as a kind of new feed resource, and it is used for aquaculture as the low algae feed that drops into high production.
Background technology
The marine alga industry of current China mainly concentrates on health food and glue is carried in industry; The poultry of feeding of some common seaweed fodders aspect the feed processing, all obtained disease-resistant yield-increasing effect preferably in the domestic animal test, but the algae Enteromorpha is used effectively seldom in harmful green tide; Seldom be applied to fishery cultivating especially as feed addictive; Therefore the progress of green tidal sea algae Enteromorpha aquatic feeds is slow, from the research of other algae feeds, can find out, marine alga not only has the advantage that promotes growth as a kind of feed of green; The more important thing is that Enteromorpha is cheap and easy to get, production is had indelible meaning.
Enteromorpha (Enteromorpha prolifera) belongs to Chlorophyta Ulvales Ulvaceae plant, mainly contains five kinds of bar Enteromorpha, intestines Enteromorpha, flat Enteromorpha, Enteromorpha, tubule Enteromorphas etc., is grown in the intertidal zone, aboundresources in the wild algae of China.Research data shows that Enteromorpha is rich in carbohydrate, amino acid, crude fibre and mineral matter, also contains fat and vitamin simultaneously.According to one's analysis, amino acid content 11.0～27.0g in every 100g Enteromorpha, fat 0.4～2.3g; Crude fibre 3.1～12.5g; All apparently higher than other algae with the marine site growth, therefore, Enteromorpha develops and applies as new feed resource micronutrient levels; Very likely can become a kind of feed resource of cheap most worthy, even possibly promote the progress in whole marine alga market.Develop this Enteromorpha resource effectively, be translated into marine algae feed resource and have earth shaking meaning with exploitation value.
Add the palatability that phagostimulant can obviously improve feed in the feed; And a large amount of amino acid that contain in the Enteromorpha are as effective phagostimulant; Mullet had very strong attracting action; Obviously improve the grazing rate of feed and reduce food-residue water body environment is polluted, also can improve the meat matter of fish simultaneously, so the food calling effect of Enteromorpha has extremely significant statistical significance equally.
In view of Enteromorpha research at present is the mensuration to Enteromorpha constituent analysis and feeding active thereof mostly; Although it is so can clear and definite its nutrient content with filter out the fish responsive to Enteromorpha; But the big valid density of workload is uncertain and be not used for practice production, is difficult to the maximization of economic benefit that Enteromorpha can be produced.The present invention is exactly on existing research basis; After scientific experimentation, the proposition of novelty is through the righttest Enteromorpha addition of test and Selection feed and then filter out best rate of body weight gain, specific growth rate; The Enteromorpha additive feed of feed coefficient is in the hope of being applied to culture fishery.
Summary of the invention
The present invention relates to the precise proportioning of bait, it is characterized in that preparing composition and process for making comprises following process with the Enteromorpha composition:
Step (one): Enteromorpha (calculating) with the Enteromorpha dry weight, the field is fished for fresh Enteromorpha seawater and is cleaned, homogenate adds cellulase 1g/kg, yeast 10g/kg, sealing place 60 ℃ of insulating boxs handle 24 hours subsequent use.
Step (two): basal feed prescription (g/kg), fish meal 300g, bean cake powder 300g, wholemeal 70g, yeast 50g, maize germ oil 50mL, soybean lecithin 15g, sodium alginate 5g, cellulase 1g.
Step (three): mixed vitamin additive capacity (mg/kg), vitamin A 50000IU, vitamin D 4000IU, vitamin E 100mg, Cobastab
150mg, Cobastab
250mg, Cobastab
65mg, Cobastab
1210 μ g, niacinamide 150mg, calcium pantothenate 50mg, lipotropin 500mg, vitamin C 500mg.
Step (four): mixed inorganic additive capacity (mg/kg), iron 100mg, copper 10mg, zinc 5mg, magnesium 10mg, iodine 1mg, manganese 10mg, potassium 100mg, calcium monohydrogen phosphate 5580mg, L-lysine salt 250mg.
[multivitamin and minerals tablet (21), Sanghai Pharmaceutical Factory, 20/kg]
Step (five): feed is handled, and the feed stirring and evenly mixing places 45 ℃ to continue fermentation 6 hours, processes granulated meal, in 45 ℃ of low temperature drying moulding.
Step (six): test feed and basal feed compare, and find the rate of body weight gain of institute's test feed, specific growth rate, and feed coefficient, feeding active is significantly increased.
The specific embodiment
The present invention relates to the precise proportioning of bait with the Enteromorpha composition, it is characterized in that preparing composition and process for making thereof and comprise the following example of executing, following embodiment can further specify the present invention, but does not limit the present invention in any way.
Embodiment:
Step (one): Enteromorpha (calculating) with the Enteromorpha dry weight, the field is fished for fresh Enteromorpha seawater and is cleaned, homogenate adds cellulase 1g/kg, yeast 10g/kg, sealing place 60 ℃ of insulating boxs handle 24 hours subsequent use.
Step (two): basal feed prescription (g/kg), fish meal 300g, bean cake powder 300g, wholemeal 70g, yeast 50g, maize germ oil 50mL, soybean lecithin 15g, sodium alginate 5g.
Step (three): mixed vitamin additive capacity (mg/kg), vitamin A 50000IU, vitamin D 4000IU, vitamin E 100mg, Cobastab
150mg, Cobastab
250mg, Cobastab
65mg, Cobastab
1210 μ g, niacinamide 150mg, calcium pantothenate 50mg, lipotropin 500mg, vitamin C 500mg.
Step (four): mixed inorganic additive capacity (mg/kg), iron 100mg, copper 10mg, zinc 5mg, magnesium 10mg, iodine 1mg, manganese 10mg, potassium 100mg, calcium monohydrogen phosphate 5580mg, L-lysine salt 250mg.
Step (five): feed is handled, and the feed stirring and evenly mixing places 45 ℃ to continue fermentation 6 hours, processes granulated meal, in 45 ℃ of low temperature drying moulding.
Step (six): test feed and basal feed compare, and find the rate of body weight gain of institute's test feed, specific growth rate, and feed coefficient, feeding active is significantly increased.
Efficiency assay with the righttest Enteromorpha addition in the ocean mullet screening feed
1 experiment material
Mullet (being collected in international bathing beach outside Shandong University ai Weihai west door), Enteromorpha (pick up from international bathing beach outside Shandong University ai Weihai west door, gather the back and clean) with seawater
2 methods and result
2.1 grazing rate test
4 aquariums are set altogether, and each aquarium mullet 20 tail is weighed behind the 24h on an empty stomach; The duration of test water temperature adopts the amount of the being satiated with food method of throwing something and feeding between (24.5 ± 1.0) ℃, throw something and feed every day three times; 10 o'clock every mornings, at 14 o'clock at noon, the bait throwing in of regularly fixing a point at 18 o'clock in afternoon; Daily ration, feeding quantity is controlled at fish body weight 2%～3%, inhales the end with siphonage before the every day of the bait throwing in for the first time and removes the residual bait of ight soil, and change water 1/5.Formal test finishes back fish empty stomach 24h and weighs by case.The test index computational methods are following:
Rate of body weight gain (%)=[(testing last fish body counterpoise-test fish body counterpoise just)/test is fish body counterpoise just] * 100%
Specific growth rate (%)=[(testing last fish body counterpoise-test fish body counterpoise just)/test fate] * 100%
Feed coefficient=bait consumption/fish total augment weight amount
The different bait of table 1 are to the influence of mullet growth
2.2 maze test
The liquid maze method is taked in this experiment; Promptly in the transparent aquarium of 80cm * 80cm * 80cm, carry out; Place the clear glass baffle plate in the aquarium and form the labyrinth, put into the dark brackish water of 25cm (salt content 1.30 ± 0.04%), 1h is with separating with gauze between A, the B before on-test; In the transition aquarium, get the A that 20 mullets are put into labyrinth aquarium one end at random, get the other end B place that puts into the labyrinth aquarium after 2.0g bait wraps up with gauze then.After waiting to test fish placement A end adaptation 5min; Removing gauze picks up counting; With 5min is unit, gets the test fish and gets into and effectively to distinguish and peck that to sting the total degree of trying be observed value, and the data of each test are with the mean+/-standard error (M ± SE) expression of each repeated observation value.The maze method test that above-mentioned steps makes an experiment respectively and organizes bait and control group bait, every kind of test bait repeats 3 times.Extent with the numerical value of test group and control group is judged the food calling effect of different proportion Enteromorpha to mullet, and negative value is represented to suppress to ingest, and induces on the occasion of expression and ingests.Analyze data with SPSS 18.0 statistical packages, adopt the t check, with P＜0.05 for remarkable food calling meaning is arranged
Compare with basal feed, different Enteromorpha addition feed gained results compared (result representes with M ± SD) result such as following table:
The feeding active contrast of table 2 test material
By the growth result of table 1 analysis mullet, add the test group one (10% Enteromorpha) of Enteromorpha additive in the feed, test group two (15% Enteromorpha); Test group three (20% Enteromorpha) growing state all obviously is superior to the control group of basal feed; The food calling effect is superior to control group equally, and the 3rd group of food calling effect that adds 20% Enteromorpha be (P＜0.01) extremely obviously, and mullet rate of body weight gain, feed coefficient, feed conversion rate all are in optimum level; Effect is best, and its integrated cost also is lower than other groups.
Visible by table 2, test group and control group difference just are, and observe t value or P value and can find out the strong and weak sequence consensus of feeding active, see that from relative indicatrix test group is all greater than 1, so test group bait all has the food calling effect to mullet.At first, discharge sequencing according to the size of mean value difference, the strong and weak order of the food calling of test bait is: test group three＞test group two＞test group one; Test group two P=0.012＜0.05, test group three P=0.000＜0.05 their food calling effect significance of explanation;
Wherein, test group three P=0.000＜0.01 explains that the food calling effect of three pairs of mullets of test group has extremely significant meaning.
Through above-mentioned test, explain that Enteromorpha is had good effect and application potential with proper proportion as additive of bait.
Claims (3)
1. the present invention relates to the precise proportioning of bait, it is characterized in that preparing composition and process for making comprises following process with the Enteromorpha composition:
Step (one): Enteromorpha (calculating) with the Enteromorpha dry weight, the field is fished for fresh Enteromorpha seawater and is cleaned, homogenate adds cellulase 1g/kg, yeast 10g/kg, sealing place 60 ℃ of insulating boxs handle 24 hours subsequent use.
Step (two): basal feed prescription (g/kg), fish meal 300g, bean cake powder 300g, wholemeal 70g, yeast 50g, maize germ oil 50mL, soybean lecithin 15g, sodium alginate 5g, cellulase 1g.
Step (three): mixed vitamin additive capacity (mg/kg), vitamin A 50000IU, vitamin D 4000IU, vitamin E 100mg, Cobastab
150mg, Cobastab
250mg, Cobastab
65mg, Cobastab
1210 μ g, niacinamide 150mg, calcium pantothenate 50mg, lipotropin 500mg, vitamin C 500mg.
Step (four): mixed inorganic additive capacity (mg/kg), iron 100mg, copper 10mg, zinc 5mg, magnesium 10mg, iodine 1mg, manganese 10mg, potassium 100mg, calcium monohydrogen phosphate 5580mg, L-lysine salt 250mg.
Step (five): feed is handled, and the feed stirring and evenly mixing places 45 ℃ to continue fermentation 6 hours, processes granulated meal, in 45 ℃ of low temperature drying moulding.
Step (six): test feed and basal feed compare, and find the rate of body weight gain of institute's test feed, specific growth rate, and feed coefficient, feeding active is significantly increased.
2. step according to claim 1; It is characterized in that: handle fresh Enteromorpha and set Enteromorpha interpolation gradient filtering out through certain method the fish rate of body weight gain; Specific growth rate; Feed coefficient is the most significantly filled a prescription, and eliminates to be not suitable for the prescription as feed addictive Enteromorpha addition, and it is used for aquaculture as the low algae feed that drops into high production the most at last.
3. step according to claim 1 is characterized in that filtering out the righttest Enteromorpha prescription feed through setting gradient.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005230A (en) * | 2013-01-18 | 2013-04-03 | 苏州大学 | Mullet fodder with balanced fatty acids |
| CN103583919A (en) * | 2013-11-26 | 2014-02-19 | 青岛嘉瑞生物技术有限公司 | Formula of enteromorpha-added myxocyprinus asiaticus puffing compound feed and production method |
| CN103636969A (en) * | 2013-11-26 | 2014-03-19 | 青岛嘉瑞生物技术有限公司 | Enteromorpha prolifera added formula and production method of sphyraenus puffed compound feed |
| CN104489326A (en) * | 2014-12-09 | 2015-04-08 | 汕头大学 | Fish feed enzyme preparation and preparation and addition methods thereof as well as fish feed |
| CN105010769A (en) * | 2014-04-24 | 2015-11-04 | 山东大学（威海） | Preparation method of aquaculture compound feed |
| CN105639209A (en) * | 2016-03-21 | 2016-06-08 | 中国水产科学研究院黄海水产研究所 | Enteromorpha type stichopus japonicus biological feed and preparation method thereof |
| CN106490307A (en) * | 2016-12-14 | 2017-03-15 | 河南牧翔动物药业有限公司 | A kind of method of antimicrobial composition fermentation Entermorpha |
| CN110583906A (en) * | 2019-10-09 | 2019-12-20 | 山东康科润海洋科技有限公司 | Enteromorpha-based crayfish compound feed and preparation method thereof |
-
2011
- 2011-11-24 CN CN2011103944092A patent/CN102406088A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005230A (en) * | 2013-01-18 | 2013-04-03 | 苏州大学 | Mullet fodder with balanced fatty acids |
| CN103583919A (en) * | 2013-11-26 | 2014-02-19 | 青岛嘉瑞生物技术有限公司 | Formula of enteromorpha-added myxocyprinus asiaticus puffing compound feed and production method |
| CN103636969A (en) * | 2013-11-26 | 2014-03-19 | 青岛嘉瑞生物技术有限公司 | Enteromorpha prolifera added formula and production method of sphyraenus puffed compound feed |
| CN103583919B (en) * | 2013-11-26 | 2014-11-05 | 青岛嘉瑞生物技术有限公司 | Formula of enteromorpha-added myxocyprinus asiaticus puffing compound feed and production method |
| CN103636969B (en) * | 2013-11-26 | 2014-11-05 | 青岛嘉瑞生物技术有限公司 | Enteromorpha prolifera added formula and production method of sphyraenus puffed compound feed |
| CN105010769A (en) * | 2014-04-24 | 2015-11-04 | 山东大学（威海） | Preparation method of aquaculture compound feed |
| CN104489326A (en) * | 2014-12-09 | 2015-04-08 | 汕头大学 | Fish feed enzyme preparation and preparation and addition methods thereof as well as fish feed |
| CN105639209A (en) * | 2016-03-21 | 2016-06-08 | 中国水产科学研究院黄海水产研究所 | Enteromorpha type stichopus japonicus biological feed and preparation method thereof |
| CN105639209B (en) * | 2016-03-21 | 2018-07-06 | 中国水产科学研究院黄海水产研究所 | A kind of enteromorpha stichopus japonicus biological feedstuff and preparation method thereof |
| CN106490307A (en) * | 2016-12-14 | 2017-03-15 | 河南牧翔动物药业有限公司 | A kind of method of antimicrobial composition fermentation Entermorpha |
| CN110583906A (en) * | 2019-10-09 | 2019-12-20 | 山东康科润海洋科技有限公司 | Enteromorpha-based crayfish compound feed and preparation method thereof |
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Application publication date: 20120411 |