CN104486953A

CN104486953A - Feed product and/or feed ingredient

Info

Publication number: CN104486953A
Application number: CN201480001612.0A
Authority: CN
Inventors: B·格伦克罗斯; N·P·普莱斯顿; S·艾尔文
Original assignee: Commonwealth Scientific and Industrial Research Organization CSIRO
Current assignee: Commonwealth Scientific and Industrial Research Organization CSIRO
Priority date: 2013-04-12
Filing date: 2014-04-11
Publication date: 2015-04-01
Also published as: MX2015014121A; AU2015201193A1; WO2014165936A1; AU2013204453A1; ECSP15009928A; AU2015201193B2

Abstract

The invention relates to a feed product or feed ingredient comprising a microbial biomass, wherein the feed product or feed ingredient is substantially free of an aquatic animal-derived protein source and/or an aquatic animal derived-lipid source. In another aspect it relates to methods or uses of the feed product or feed ingredient as described.

Description

Feed product and/or feed ingredient

Technical field

The present invention relates to a kind of feed product for water biological species or feed ingredient, the described feed product of use or component and raise the method for water biological species and the purposes of feed product of the present invention or component.

Background technology

The expansion of whole world aquaculture makes culture fishery study the feeding system of more densification to meet growing demand.But these densification rearing conditions utilize the cultural method highly depending on and use synthesizing formula feed instead of more natural food source (such as pond life) usually.Usually, aquatic products formula forage (such as shrimp feed) depends on and uses fishery resources (such as fish meal and fish oil) as nutraceutical important sources.

But one of obvious shortcoming of mixed feed is its main operation cost that is expensive and that therefore usually occupy in densification feeding system.In fact, the cost of these fishery resources constantly rises because of global excessive amount of fishing, and at present, this constitutes a threat to by the cost benefit and long-term sustainable that use fish meal and other aquatic products powder and fish oil and the oily culture fishery be used for of other aquatic products.Therefore, boost productivity and reduce feed cost to the commercialization of aquatic products industry and long-term viability, to boost productivity and to reduce feed cost most important.

Culture fishery is also becoming the key areas preparing high protein diet.The preparation of high protein diet needs the high-quality feed with high protein content, and it not only should contain all required nutrients, also should containing supplemented by additives to keep organism healthy and to be conducive to growth.May to animal pest with growth-promoting additive (such as hormone and antibiotic) fortified feed, and, from the angle viewpoint of publilc health, antibiotic use and the consequence that produces in shrimp aquaculture thereof because of time human consumer in the face of antibiotic residue potential risk and receive a large amount of negative concerns.

In culture fishery, obviously need substituting, low cost, environmental friendliness and continuable main nutrient (such as protein, lipid and growth promoter) source.

But the trial substituting the raw material in aquatic feeds has problems, this is because the Major Nutrient standard of each species must be kept to guarantee output and cost parameter active balance always.

Microbial biomass has been confirmed as a kind of nutriment source for maintaining aquaculture.See Australian Patent 2008201886 (CSIR O) and Australian Patent 2009225307 (CSIR O), wherein this application reports a kind of method of the improvement for the preparation of microbial biomass.Should particularly point out, in AU2008201886 and AU2009225307, the powder that aquatic animal derives and the lipid that aquatic animal derives are considered to the key component of aquatic feeds composition and feed ingredient in the prior art.

The present inventor now have been surprisingly found that, substantially containing fishery resources or be derived from the resource (such as fish meal and/or fish oil) of aquatic animal and the aquatic feeds component comprising microbial biomass can maintain water biological species or provide the substitute of a kind of prior art fodder compound or feed ingredient.

Summary of the invention

In a first aspect of the present invention, a kind of feed product or feed ingredient are provided, it comprises microbial biomass, wherein, described feed product or feed ingredient are substantially free of the resource that one or more are derived from fishery, described in be derived from fishery resource comprise the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal.

In a second aspect of the present invention, a kind of feed product or feed ingredient are provided, it comprises microbial biomass and also comprises one or more lipid source being derived from plant and/or its mixture, wherein, described feed product or feed ingredient are substantially free of the resource that one or more are derived from fishery, and the resource being derived from fishery comprises the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal.

In a third aspect of the present invention, a kind of feed product for aquatic animal or feed ingredient are provided, its lipid source comprising microbial biomass and be derived from plant, wherein, the lipid source being derived from plant exists with a certain amount of with microbial biomass together with protein source, to make described feed product or feed ingredient be balanced in nutrition for aquatic animal, described protein source is selected from the source except the protein source being derived from fishery.

In a fourth aspect of the present invention, a kind of feed product for aquatic animal or feed ingredient are provided, it comprises microbial biomass and closes the lipid source being derived from plant, wherein, the lipid source being derived from plant exists with a certain amount of with microbial biomass together with protein source, balanced in nutrition for aquatic animal to make described feed product or feed ingredient, described protein source is selected from the source except the protein source being derived from fishery, wherein, described feed product or feed ingredient also comprise the lipid of proportioning balanced in nutrition and/or oily or its mixture, protein source, amino acid and/or its mixture.

In in the 5th, the present invention relates to first, second, third or the feed product of fourth aspect or feed ingredient as aquatic feeds or the feed ingredient purposes for water biological species.

In in the 6th, the present invention relates to a kind of method of raising water biological species, it comprises provides of the present invention first, second, third or the feed product of fourth aspect or the step of feed ingredient to water biological species member.

In in the 7th, the present invention relates to a kind of method of raising water biological species, it comprises provides to water biological species the feed product or feed ingredient that are substantially free of fishery resources, and wherein, described feed product or feed ingredient are feed product or the feed ingredient of the present invention first or second aspect.

In eighth aspect, the present invention relates to a kind of water biological species, prepared by its method by the third aspect, preferably relates to small shrimp (shrimp) or prawn (prawn).

In in the 9th, the present invention relates to that a kind of described feed product or feed ingredient are substantially free of fishery resources for the preparation of the feed product of water biological species or the method for feed ingredient, described method comprises:

Microbial biomass as described herein is provided; And

Be added in microbial biomass by one or more components of proportioning balanced in nutrition, described component is selected from the adhesive, Kohlenhydrate, the protein source being selected from the source except the protein source being derived from fishery, the lipid being selected from the source except the lipid being derived from fishery, vitamin, mineral matter and/or its mixture that are suitable for expection water biological species.

Definition

In whole description, should be understood that defined term has implication understood by one of ordinary skill in the art.

Except as otherwise noted, should be understood that all percentage as herein described refers to % (w/w), based on air-dry mass/mass meter.

Term " fishery resources ", as will be appreciated by a person skilled in the art, be understood to include the key element (element), product, composition or the resource that are derived from aquatic ecosystem or environment, comprise be derived from aquatic ecosystem or environment bacterial strain, species, population and colony (stock), and comprise the resource relevant with fish, shell or other aquatic animals, be such as derived from the protein source (comprising fish meal, cuttlefish powder or krill meal) of aquatic animal and be derived from the lipid source (comprising fish oil, cittle fish oil or krill oil) of aquatic animal.

Term used herein " is substantially free of ", as will be appreciated by a person skilled in the art, be interpreted as meaning fodder compound or feed ingredient comprise fishery resources (be such as derived from the protein source of aquatic animal and/or be derived from the lipid source of aquatic animal) and do not exist completely, exist with trace content/concentration or with lower than or to be less than in prior art fodder compound the fodder compound of amount/concentration existence or the feed ingredient of amount/concentration that institute usually uses or uses.

Term " aquatic animal " is understood to include and is selected from fish, shellfish and/or molluscan marine animal and fresh water animals.

Term " is derived from the lipid source of aquatic animal " and is understood to include the lipid source being derived from fishery, such as fish oil, krill oil and/or cittle fish oil.

Term " is derived from the protein source of aquatic animal " and is understood to include the protein source being derived from fishery, such as fish meal, cuttlefish powder and/or krill meal.

Term " is selected from the lipid in the source except the lipid being derived from fishery " and is interpreted as the Lipid sources meaning to be derived from plant or non-aquatic animal.

Term " proportioning balanced in nutrition " is interpreted as the proper ratio meaning concrete selected component in feed product of the present invention or feed formula, and it effectively can maintain the growth of relevant water biological species.

Term used herein " microbial biomass " refers to the solid biomass comprising microorganism and cellulosic organic matter.Microorganism is mainly bacterium, but micro-algae, saccharomycete, protist and fungi also can be used as pollutant to be existed on a small quantity.

In whole description, word " comprise (comprise) " or its modified example as " comprising (comprises) " or " comprising (comprising) " be interpreted as meaning comprising as described in key element, entirety or step, or the group of key element, entirety or step, but the group not getting rid of any other key element, entirety or step or key element, entirety or step.

Except as otherwise noted, in whole description, referential data should be considered as meaning " about " for described numerical value.Term " about " is used for the standard deviation that representative value comprises the error for the equipment or method measuring this value, or the variation existed in experiment value (variation).

In a preferred form, when feed product or feed ingredient also comprise the lipid source being derived from plant, the lipid source being derived from plant can form feed product or the feed ingredient of about 1% to about 10%, preferably about 1% to about 5% or more preferably from about 2% to about 4% or about 2.5%.

In one embodiment, microbial biomass form about 0.5% to about 40%, about 0.5 to about 30%, about 0.5 to about 25%, preferably about 1% to about 20% or about 1% to about 15% or about 3% to about 12% or the feed product of about 3% to about 10% or feed ingredient.

The lipid source being derived from plant can be selected from vegetable oil, soybean oil, mustard caul-fat, linseed oil or linseed oil, hemp-seed oil, Canola oil (rapeseed oil), pumpkin seed oil, purslane, Purple Perilla Seed Oil, walnut oil and/or its mixture.

In a preferred form, the lipid source being derived from plant can have the omega-fatty acid being about greater than 1:1, preferably about 3:1 to 1:1, preferably about 3:1 to 2:1, preferably 1:1 to about 2:1: ω-6 aliphatic acid ratio.

In another embodiment, the lipid source being derived from plant can have the omega-fatty acid of preferably about 1:2 to 1:7, preferably about 1:2 to 1:5, preferably 1:2 to about 1:3: ω-6 aliphatic acid ratio.

In a form, the protein source be selected from except the source of the protein source being derived from fishery is also selected from: soy meal, poultry powder, lupin (grain) powder (lupin (kernel) meal) and wheat gluten and/or its mixture.

In a preferred form of the invention, feed product or feed ingredient comprise microbial biomass, and also comprise the lipid source being derived from plant, wherein, be derived from feed product or feed ingredient that vegetable lipid source forms about 1% to about 10%, preferably about 1% to about 5% or more preferably from about 2% to about 4% or about 2.5%, and wherein, feed product or feed ingredient are substantially free of the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal.

In a preferred form of the present invention, feed product or feed ingredient comprise microbial biomass and the combination of lipid source being derived from plant, wherein, feed product or feed ingredient comprise about 1% to about 10%, preferably about 1% to about 5% or more preferably from about 2% to about 4% the lipid source being derived from plant with about 0.5% to about 40%, about 0.5 to about 30%, about 0.5 to about 25%, preferably about 1% to about 20% or about 1% to about 15%, about 3% to about 12% or the combination of microbial biomass of about 3% to about 10%.

In another form of the present invention, feed product or feed ingredient comprise microbial biomass and the combination of lipid source being derived from plant, wherein, feed product or feed ingredient comprise about 1% to about 10%, the preferably lipid source being derived from plant and about 0.5% to about 40% of about 1% to about 5% or more preferably from about 2% to about 4%, about 0.5 to about 30%, about 0.5 to about 25%, preferred about 1% to about 20%, or about 1% to about 15%, about 3% to about 12%, or the combination of the microbial biomass of about 3% to about 10%, and described feed product or feed ingredient are substantially free of the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal.

In one embodiment of the invention, feed product or feed ingredient comprise microbial biomass, wherein, microbial biomass forms about 0.5% to about 40%, about 0.5 to about 30%, about 0.5 to about 25%, preferred about 1% to about 20%, or about 1% to about 15%, about 3% to about 12%, or the feed product of about 3% to about 10% or feed ingredient, and wherein said feed product or feed ingredient also comprise the lipid source being derived from plant, the lipid source being wherein derived from plant forms about 1% to about 10%, preferably about 1% to about 5% or the feed product of more preferably from about 2% to about 4% or feed ingredient, and wherein said feed product or feed ingredient are substantially free of the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal.

In a preferred form, feed product of the present invention or feed ingredient can be substantially free of fishery resources, wherein, fishery resources can be the protein source (such as fish meal, cuttlefish powder or krill meal and/or its mixture) being derived from aquatic animal and/or the lipid source (such as fish oil, krill oil or cittle fish oil and/or its mixture) being derived from aquatic animal, and it is present in prior art fodder compound usually.

In substituting embodiment, the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal can form about 0% to about 20%, about 0.1% to about 15%, about 0.5% to about 12%, about 0.75% to about 10%, about 1% to the feed product of about 7.5%, about 1.5% to about 5% or feed ingredient.

In another embodiment, feed product or feed ingredient comprise microbial biomass, and wherein, microbial biomass forms about 0.5% to about 40%, about 0.5 to about 30%, about 0.5 to about 25%, preferred about 1% to about 20%, or about 1% to about 15%, about 3% to about 12%, or the feed product of about 3% to about 10% or feed ingredient, and wherein said feed product or feed ingredient also comprise the lipid source being derived from plant, wherein, the lipid source being derived from plant forms about 1% to about 10%, preferably about 1% to about 5% or the feed product of more preferably from about 2% to about 4% or feed ingredient, and wherein said feed product or feed ingredient also comprise the lipid source being derived from aquatic animal, the lipid source being wherein derived from aquatic animal forms about 0.1% to about 15%, about 1% to about 10%, about 1% to about 5%, and about 1% to about 3.5% or the feed product of about 1% to about 2.5% or feed ingredient, and wherein said feed product or feed ingredient are substantially free of the protein source being derived from aquatic animal.

In another embodiment, feed product or feed ingredient comprise microbial biomass, and its microbial biomass forms about 0.5% to about 40%, about 0.5 to about 30%, about 0.5 to about 25%, preferred about 1 % by weight to about 20%, or about 1% to about 15%, about 3% to about 12%, or the feed product of about 3% to about 10% or feed ingredient, and wherein said feed product or feed ingredient also comprise the lipid source being derived from plant, the lipid source being wherein derived from plant forms about 1% to about 10%, preferably about 1% to about 5% or the feed product of more preferably from about 2% to about 4% or feed ingredient, and wherein said feed product or feed ingredient also comprise the protein source being derived from aquatic animal, the protein source being wherein derived from aquatic animal forms about 0.1% to about 15%, about 1% to about 10%, about 1% to about 5%, and about 1% to about 3.5% or the feed product of about 1% to about 2.5% or feed ingredient, and wherein said feed product or feed ingredient are substantially free of the lipid source being derived from aquatic animal.

In an alternative embodiment, feed product or feed ingredient comprise microbial biomass, wherein microbial biomass forms about 0.5% to about 40%, about 0.5 to about 30%, about 0.5 to about 25%, preferred about 1 % by weight to about 20%, or about 1% to about 15%, about 3% to about 12%, or the feed product of about 3% to about 10% or feed ingredient, and wherein said feed product or feed ingredient also comprise the lipid source being derived from plant, the lipid source being wherein derived from plant forms about 1% to about 10%, preferably about 1% to about 5% or the feed product of more preferably from about 2% to about 4% or feed ingredient, and described feed product or feed ingredient also comprise the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal.The protein source being derived from aquatic animal can form about 0.1% to about 15%, about 1% to about 10%, about 1% to about 5% and about 1% to about 3.5% or the feed product of about 1% to about 2.5% or feed ingredient.The lipid source being derived from aquatic animal can form about 0.1% to about 15%, about 1% to about 10%, about 1% to about 5% and about 1% to about 3.5% or the feed product of about 1% to about 2.5% or feed ingredient.

In a preferred form, water biological species can be selected from fish, crustacean and/or mollusk.In a form, described fish is selected from atlantic salmon, barramunda and cobio; Crustacean is selected from small shrimp or prawn, lobster and crab; And mollusk can be selected from oyster, scallop and abalone.

In one embodiment, feed product of the present invention or feed ingredient also can comprise adhesive, such as gluten, alginate or starch; Other sources of protein, such as soy meal, poultry powder, feather fan bean powder, glutelin powder; Be rich in carbohydrate, particularly rich amyloid component, such as wheat flour, rice bran, tapioca starch, ground rice, cornflour or corn flour; Other Lipid sources; Be suitable for the vitamin mixtures of expection water biological species; Be suitable for the mineral mixture of expection water biological species; And other nutrition, medicine or growth replenishers.

Microbial biomass used in the present invention can use the carbon source preparation being selected from refuse, high power capacity, low value Agricultural Materials and agricultural wastes.Low value Agricultural Materials can comprise the product, accessory substance or the waste stream (such as filter mud, field trash (canetop), syrup or bagasse) that are produced by processing sugarcane.Other sources comprise by processing paddy, wheat, triticale, corn, Chinese sorghum, cassava, product, accessory substance or waste stream that oilseeds (comprising the canola dregs of rice and lupin shell) produce and the elevator dust (elevator dust) that produced by crop-processing equipment.Other sources of carbon and nitrogen can comprise the production waste and wine brewing vinasse product that are produced by feed-processing plant.Carbon source can be the field trash (canetop) having ground or sieved in instantiation.

In preferred embodiments, prepared by the accessory substance that microbial biomass used in the present invention can be produced by processing sugarcane or waste stream.In a preferred form, the accessory substance produced by processing sugarcane or waste stream can comprise bagasse, field trash, filter mud or syrup.In a preferred form, microbial biomass is made up of cassava or field trash or syrup.

Microbial biomass used in the present invention can use nitrogenous source to prepare, and described nitrogenous source can be continuable nitrogenous source on economy and environment such as urea, ammonia, ammonium nitrate, ammophos, comprises the organic nitrogen source of the water discharged by aquaculture pond.Other nitrogenous sources can be added in cultivating system.In cultivating system, the concentration of total nitrogen can be about 10mg/L to about 80mg/L, 20mg/L extremely about 70mg/L, 30mg/L extremely about 60mg/L, 40mg/L to 60mg/L or 40mg/L to 50mg/L when cultivating and starting.In a further preferred embodiment, the concentration of total nitrogen can be about 40mg/L to about 70mg/L when cultivating system starts.

In a preferred embodiment, microbial biomass used in the present invention can use the initial C:N (carbon: nitrogen) of about 2:1 to 20:1,3:1 to 18:1,4:1 to 18:1,5:1 or 18:1 or 6:1 to 18:1 than preparation.Other C:N ratios can be 7:1,8:1,9:1,10:1,11:1,12:1,13:1,14:1,15:1,16:1,17:1 or 18:1.Preferred 6:1 to 18:1, more preferably 12:1.

Naturally occurring microorganism in water used in the microbe-derived cultivating system for generation microbial biomass in microbial biomass.In certain embodiments, produce water used in the cultivating system of microbial biomass be from obtain in any water source comprising marine site and waters, river mouth without processing and the recirculated water that produces of the seawater of filtered, the waste water of discharging from aquaculture pond or previously cultivation.

Microbial biomass used in the present invention can use the mixed population preparation of microorganism comprising micro-algae, saccharomycete, fungi, protist and bacterium, particularly wherein microorganism mixed population comprises bacterium, and more especially wherein microorganism mixed population is essentially bacterium.

In one embodiment, microbial biomass used in the present invention comprises the mixed population of microorganism, and described microorganism comprises micro-algae and bacterium.In a preferred embodiment bacterium exist amount with dry basis be about 1 % by weight to about 50 % by weight, about 5 % by weight to 40 % by weight, about 5 % by weight to about 25 % by weight.In another embodiment, the amount that exists of micro-algae with dry basis be about 0.1 % by weight to about 50 % by weight, about 0.1 % by weight to about 40 % by weight, about 0.1 % by weight to about 30 % by weight, about 0.1 % by weight to about 25 % by weight, about 0.1 % by weight to about 20 % by weight, about 0.1 % by weight to about 15 % by weight, about 0.1 % by weight to about 10 % by weight, about 0.1 % by weight to about 5 % by weight.

In another embodiment, the microbial biomass used in the present invention comprises the mixed population of microorganism, described microorganism comprises the combination of micro-algae and bacterium, wherein, bacterium exist amount with dry basis for about 1 % by weight to about 50 % by weight, about 5 % by weight to 40 % by weight, about 5 % by weight to about 25 % by weight, and the amount that exists of micro-algae with dry basis for about 0.1 % by weight to about 50 % by weight, about 0.1 % by weight to about 40 % by weight, about 0.1 % by weight to about 30 % by weight, about 0.1 % by weight to about 25 % by weight, about 0.1 % by weight to about 20 % by weight, about 0.1 % by weight to about 15 % by weight, about 0.1 % by weight to about 10 % by weight, about 0.1 % by weight to about 5 % by weight.

In yet another embodiment, the microbial biomass used in the present invention comprises the mixed population of microorganism, and wherein said microorganism comprises the mixed population of bacterium.In certain embodiments, the mixed population of microorganism comprise the bacterium being greater than 50 % by weight mixed population, be preferably greater than 60 % by weight or be greater than the mixed population of bacterium of 70 % by weight, the mixed population of the bacterium more preferably greater than 80 % by weight or 85 % by weight.In certain embodiments, microbial biomass is essentially bacterium living beings matter.

Micro-algae quantitatively can based on the chlorophyll a of microbial biomass, and bacterium quantitatively can based on muramic acid content.

Microbial biomass used in the present invention can use the nutrients of optimum level to prepare, and described nutrients is selected from phosphate, silicate and composition thereof.Described phosphate can be selected from KH ₂pO ₄, calcium superphosphate, two calcium superphosphate, double superhosphate, MAP, Diammonium phosphate (DAP), rock phosphate and Agra (Agras).

Can with a certain amount of interpolation KH ₂pO ₄to provide the P:N ratio of about 1:1 to 20:1, about 2:1 to 18:1, about 2:1 to 16:1, about 2:1 to 14:1, about 2:1 to 10:1 or about 2:1 to 8:1.Other P:N ratios can be 2:1 to 6:1,5:1,4:1,3:1, or are 2:1 to 3:1 in an example.

Sodium metasilicate, sodium metasilicate (Na can be selected from preparing silicate used in microbial biomass ₂siO ₃5H ₂o), waterglass, potassium silicate.Can with a certain amount of interpolation Na ₂siO ₃5H ₂o is to provide about 0.1:1, about 0.3:1, about 0.6:1, about 1:1, about 1.5:1 to the Si:N ratio of about 1.7:1, about 1.9:1 to 2.0:1.

Accompanying drawing explanation

Below now only describe the present invention by way of example with reference to the accompanying drawings, described accompanying drawing will be explained below.

Fig. 1 describes the productive rate (g/kL) of the microbial biomass in each process of the embodiment 1 detecting different sugar cane waste stream.What X-axis marked is carbon source used in microbial biomass preparation.A to F represents the daily ration condition (diet condition) gone out as listed in table 1.

Fig. 2 represents and often plants the experiment Diet Formula of feed and total component demand based on the 2000g described in embodiment 2.

Fig. 3 illustrates initial weight (g) and final mass (g), weightening finish (g), rate of body weight gain (g/ week), relative growth increase % (RGI) and the FCR (feed conversion rate) of the prawn of each of the process daily ration in feeding embodiment 2.

Fig. 4 describes the prawn fed with the microbial biomass prepared by different sugar cane waste stream of 10% inclusion level as described in Example 2 through the growth rate (g/ is all) of 42 days.Substrate daily ration is not containing microbial biomass.

Fig. 5 represents the experiment daily ration preparation that embodiment 3 is used.

Fig. 6 illustrates that initial weight (g) and the final mass (g) of the prawn of each of the process daily ration in feeding embodiment 3, increase weight (g), rate of body weight gain (g/ week), relative growth increase % (RGI) and FCR (feed conversion rate) and % survival rate.MB is microbial biomass of the present invention.

Fig. 7 be represent as shown in Figure 5 and the horizontal percentage of bioactive inclusions (X-axis) of as described in Example 3 Diet Formula A to K relative to the figure of the growth rate (g/ week) (Y-axis) of prawn.

Fig. 8 illustrates the experiment daily ration preparation that embodiment 4 and 5 is used.

Fig. 9 illustrates that initial weight (g) and the final mass (g) of the prawn of each process daily ration in feeding embodiment 4, increase weight (g), rate of body weight gain (g/ week), relative growth increase % (RGI) and FCR (feed conversion rate) and % survival rate.MB is microbial biomass of the present invention, and FM is fish meal, and FO is fish oil, and LO is linseed oil.% represents the inclusion percentage in often kind of feed process as described in Example 4 and as shown in Figure 8.

Figure 10 describes the rate of body weight gain (g/ week) of the prawn of each process daily ration of feeding in tentative experiment in 63-days as described in Example 4 and as shown in Figure 8.FM: fish meal, FO: fish oil, MB: microbial biomass, LO: linseed oil.

Figure 11 illustrate tentative experiment in the 63-described in embodiment 5 days beginning and at the end of the initial weight (g) of prawn that produced by often kind of process and final mass (g), weightening finish (g), rate of body weight gain (g/ week), relative growth increase % (RGI) and FCR (feed conversion rate) and % survival rate.FM: fish meal, FO are: fish oil, MB: microbial biomass, LO: linseed oil.% represents the inclusion percentage in each feed process as described in Example 5.

Figure 12 describes the rate of body weight gain (g/ week) of the prawn of each process daily ration of feeding in tentative experiments in 63-days as described in Example 5 and as shown in Figure 8.FM: fish meal, FO: fish oil, MB: microbial biomass, LO: linseed oil.

Detailed description of the invention

The present invention is existing to be described with reference to following examples, and following examples are only for illustrating the preferred embodiments of the invention.Following examples do not limit scope of the present invention as defined by the following claims.

Embodiment

embodiment 1

extended carbon source is utilized to prepare microbial biomass

Be pre-formed this experiment to determine whether the scope of sugar cane waste stream can be used as the carbon source of the microbial biomass prepared containing the biological active component that can accept output.

Nitrogen level used is 40g/L and C:N ratio is 12:1.Tapioca starch is used as the carbon source of control treatment.

method-cultivation microbial biomass

Described experiment is that 40mg/L and C:N forms (table 1) than the five kinds of carbon sources (syrup, field trash, bagasse, filter mud and tapioca starch contrast) being 12:1 by N concentration.

Other process the field trash detected under identical N concentration and C:N ratio, but described field trash adds as pulverizing crude product (being similar to husk).For each process, all there is the tank of four 2400L.In all process, single batch adds nutrients and is converted into the biology conversion of collectable living beings post-evaluation in 35 days.

Before one week, the hydrosol (70g) is joined in the seawater of each tank (2400L), to promote the foundation of clear water condition (green water condition) in experiment beginning.Be circular glass fiber groove for cultivating the tank of microbial biomass, it is positioned at the below of gardening passage room (tunnel house) side.Passage room makes the minimizing attenuation of natural daylight and water temperature can be made to remain between 25 to 33 DEG C.

Water is undertaken circulating by mixing channel and uses in pumped back tank, to guarantee that in all tanks, microbiologic population is identical with Wei Zao group and nutraceutical concentration before experiment starts.Once amount reproduction, stop circulation and the urea (nitrogenous source) of allocation process and carbon source are added in single tank.

Add nitrogenous source and urea adds to provide the N of 40mg/L (each tank 99 to 194g urea), and add carbon source to provide the C:N ratio of 12:1.In addition, by KH ₂pO ₄be added in all tanks to obtain the P:N ratio of about 5.0:1.Nutrients in tank and granular material are by violent aeration (aeration) and use the gas lift unit in each tank and keep mixing and suspend.

Use weekly thick squeegee (coarse broom) to clean flume wall and bottom gently, minimize to make the accumulations in flume surface.In addition, reading is measured every day to determine dissolved oxygen, pH, temperature and salinity.

At experimental session, water temperature is 29.8 ± 0.46 DEG C (scope is 26.8 to 32.4 DEG C).The salinity of water is 35.2 ± 0.89mg/L (scope is 34.2 to 36.5mg/L).

At the end of experiment (35 days), every day gathers 4 or 5 tanks by baleen formula filter (baleen filter).Record the total output weight in wet base of each tank, the representative increment of 100g is dried at 105 DEG C, with the dry productive rate of working sample.By labelled for the sample of each tank and store in a reservoir with freeze drying, remainder is labelled and store in mode (in bulk) by the gross.At all microbial biomass collected are stored in-20 DEG C.

Process tested by table 1.

result

This experiment is determined, the sugar cane waste stream of certain limit can be used as the carbon source for the preparation of microbial biomass of the present invention.The variable level of output is relevant with each sugar cane waste stream, as Fig. 1 to paint output prepared by bagasse and field trash the highest.

embodiment 2

evaluate the effect of a series of microbial biomass prepared by sugar cane waste stream

The object of this experiment is the biologically active potentiality of a series of microbial biomass prepared by Evaluation operation example 1, and embodiment 1 uses sugar cane waste product as carbon source.Different product to be included in test daily ration with the inclusion level of 10% and to compare with reference to daily ration through six Zhousheng long sides and basis prawn.

method

These experiment needs 28 tanks (100L).Have 7 kinds of daily ration process, often kind is repeated four times.This test carries out 42 days.About 2000 young prawns (~ 2g) without selection (W) colony (stock) are distributed to large seawater groove (~ 10000L).These prawns are kept in this tank before distributing to experiment.Before distributing to experimental tank, the once commercially available particle of feeding prawn every day (by Ridley Aquafeeds Ltd, the Ridley PrawnGrower that Nerangba, Qld, Australia manufacture).

Before experiment starts, 40 prawns are weighed determine average ± SD weight.5 prawns individual based on all distribution in average ± 0.5 × SD weight are dispensed to each in 28 tanks.Prawn is weighed with the accuracy of minimum 0.1g.Sacrifice represent each of initial population 10 prawns to carry out composition analysis and to remove internal organ sample, to collect and at being stored in-80 DEG C.Then be transferred to indoor 100L tank from outdoor tank, water temperature is arranged to 28 DEG C.Described animal adaptive system five days.Prawn feeding every day (use commercial diet) is once with all feeds consuming and do not consume of satisfied record during this period.At the end of this laundering period, prawn is weighed again the weight recording number of days-0.The flow of experimental system maintenance ~ 1L/min, object keeps making water temperature be maintained at about 28 DEG C in experimental session.

7 kinds of formulas (basis and A1 to A6) (Fig. 2) of preparation experiment.When not in use, at all feeds will be stored in-20 DEG C.The feed used is based on standard, the basic components feed (see Fig. 2) of higher protein.Use standard prawn feed preparation method prepares often kind of feed of about 2000g.Before all components of mixing in mixing upright planetary agitator (Hobart, Sydney, NSW, Australia), prepare often kind of daily ration by guaranteeing all batchings to be milled to < 750 μm.Then in mixed process, add water to form dough, described dough carries out spiral compacting (Dolly, La Monferrina, Castell ' Alfero, Italy) by the mouth mould of 3mm and cuts into the particle that length is about 6mm subsequently.Then particle dries 24h at 40 DEG C.When there is no feeding, at daily ration being kept at-20 DEG C.

result

This research shows, there are significant biologically active potentiality in each of microbial biomass prepared in using sugar cane waste stream as the embodiment 1 of carbon source.But two kinds of sugar cane waste streams and syrup are prepared with bagasse has more significant bioactive product compared with other, as shown in Figure 3.Especially, RGI% (the Relative Growth Index% of process A2, relative growth index %) be 150% (syrup on basis+10%) and the RGI% of process A3 is 135% (bagasse on basis+10%), it shows significantly larger biologically active.

In addition, as shown in Figure 4, which show the gained growth rate of prawn, described prawn feeding has the daily ration 42 days of the various microbial biomass prepared by different sugarcane of 10% inclusion level, daily ration+10% syrup A2 and basal diet+10% bagasse A3 based on the daily ration/microorganism biological quality sample promoting optimum growh.The basal diet shown in the graph is not containing microbial biomass.

embodiment 3

The evaluation of the microbial biomass in black Tiger Prawns Penaeus monodon (black tiger prawns Penaeus monodon) feed and the bioactive properties of krill meal

The object of this research is the best inclusion rate (inclusion rate) of the microbial biomass determining to be prepared as carbon source by bagasse and compares the performance of microbial biomass and krill meal.

method

Collect about 2000 and transfer to laboratory, CSIR O Cleveland (CSIR O Cleveland Laboratories, QLDAustralia) without selecting the black Tiger Prawns childhood (~ 2g) of colony and supporting shrimp field from locality.Described shrimp will assign to 10000L hot water storgae, and before being divided into experimental tank, preserve 3-4 days at about 28 DEG C in two 2500L tanks of southern growth experiment room (Southerngrowth lab).During this period, described shrimp feeding every day twice commercial particulate (by Ridley Aquafeeds Ltd, the Ridley Prawn Grower that Nerangba, Qld, Australia manufacture).Weigh all prawns and be divided into size class, described size class will be selected from for the close dimensional scope of testing.The experimental tank of each 100L puts 5 prawns in a suitable place to breed, after putting 5 days in a suitable place to breed, again weighs prawn to start to test (number of days 0).This experiment needs 44 tanks (11 kinds process repetition 4 times).Temperature in experimental system is maintained at about 29 DEG C, and the flow of hot sea water is 0.6L/min.Monitor the temperature in each tank every day, and, immerse the temperature of whole group of data logger record in 6 random tanks.

Microbial biomass and the krill meal of 6 kinds of daily ration inclusion levels (0,3,6,9,12 and 15%) in simple basic components are compared in this experiment.0% inclusion process is common to two kinds of products, amounts to 11 kinds of process.Often kind of process repetition 4 times is also randomly assigned to 44 tanks with the design of completely random.Microbial biomass used is in this experiment the bagasse process (A3) of embodiment 2.

By feed formulation become be essentially wait nitrogen (isonitrogenous) and grade fat (isolipidic).According to standard prawn feed, preparation method prepares feed.In mixing upright planetary agitator (Hobart, Sydney, NSW, Australia) before all batchings of mixing, by guaranteeing that all batchings are milled to < 750 μm and prepare often kind of daily ration.Then in mixed process, water is added to form dough, described dough carries out spiral compacting (Dolly by #14 mouth mould (2.9mm) subsequently, La Monferrina, Castell ' Alfero, Italy) and the particle (except modifying according to original animal size) cut into about 5mm.Before it is required, at all feeds are kept at-20 DEG C.

Fig. 5 indicates and tests daily ration preparation A to K.Growth parameter(s) (weight and the rate of gain) data of Fig. 6 display often kind of process gained, the mean value calculation repeating to test by 4 times.

Parameter in Fig. 6 comprises the final percentage survival of the initial weight (g) of each prawn and each prawn weight when process 15 days and 35 days, each prawn rate of gain weekly and often kind of process A to K when experiment 15 days and 35 days.

result

Fig. 6 and Fig. 7 demonstrates weight and the rate of gain of the prawn carrying out often kind of process.The krill meal that microbial biomass and the inclusion level of 3% inclusion are up to < 10% has identical effect.Inclusion level is more than 10%, microbial biomass promotes that compared with the krill meal of similar level higher levels of growth is (see Fig. 6, be compared with 5.51g/ prawn with the weight in process K when 35 days, in process F 35 time sky weight be 6.48g/ prawn).

embodiment 4

assessment valency is substituting the microorganism in prawn daily ration in all aquatic derivative Animal resources completely the application of living beings

This experiment not only detects the possibility of all fish meal content of alternative prawn daily ration but also detects the possibility substituting fish oil.Success in this respect shows, microbial biomass can be used in offsetting and substituting the relevant performance losses of any fishery resources completely.

method

This experiment needs 120 tanks (100L).Have 10 kinds of process, 4 kinds by repetition 5 times, remaining 6 kinds are repeated 4 times.This experiment carries out 42 days.Again check 14 days and 28 days.About 2000 prawn childhood (~ 5g) are taken from CSIR O facilities Rossman Pty Ltd (Alberton, QLD Australia) CSIR O equipment of being arranged in research center, Bu Laibi island (Bribie Island ResearchCentre) to and are distributed to 2 large accumulator tanks (~ 5000L).These prawns are kept in this tank before distributing to this experiment.Prawn feeding every day commercial particulate (by Ridley Aquafeeds Ltd, the Ridley PrawnGrower that Nerangba, Qld, Australia manufacture) is until distribute to experimental tank.40 prawns are weighed to determine average ± SD weight before experiment starts.Six prawns individual based on all distribution in average ± 0.5 × SD weight are distributed to each in 60 tanks.Prawn is weighed with minimum 0.1g accuracy.Also kill 9 prawns representing each initial population, with carry out component analysis-according to 3 sample sacks altogether 3 sample sacks preserve.Experimental system remains on ~ flow of 500L/min, and object keeps the water temperature in experimental session to be about 29 DEG C.Monitor the temperature in each tank every day, and immerse the temperature of whole group of data logger record in 6 random tanks.

10 kinds of daily rations (Fig. 8) of preparation experiment.When not in use, at all feeds will be stored in-20 DEG C.Often kind of feed uses standard prawn feed preparation method to prepare about 1000g.In mixing upright planetary agitator (Hobart, Sydney, NSW, Australia) before all batchings of mixing, by guaranteeing that all batchings are milled to < 750 μm and prepare often kind of daily ration.Then in mixed process, add water to form dough, described dough carries out spiral compacting (Dolly, LaMonferrina, Castell ' Alfero, Italy) by the mouth mould of 3mm and cuts into the particle that length is about 6mm subsequently.Then particle dries 24h at 40 DEG C.When there is no feeding, at daily ration is kept at-20 DEG C.

In experimentation, prawn feeding every day twice is to reach satiety, in experimental session continuous 7 days one week.Obvious satiety is determined by supply feed excessive a little.The quantity of food (estimating the forage volume do not eaten) of record nursing every day.Calculate the feed do not eaten after feeding, described calculating is for estimating the amount of not eating feed and for adjusting quantity delivered ensuing every day.Careful record and arrive casting off a skin of any prawn at a glance and just removed.

result

This experiment shows, in Rinsing Area system, in prawn daily ration, the minimizing of fish meal causes growth to reduce.In addition, replacing fish oil completely also causes growth to reduce.But this experiment also shows, microbial biomass shows for offsetting and substituting the possibility that any fishery resources utilize relevant performance losses completely.Microbial biomass is not only offset by the loss eliminating fish meal and fish oil generation, and acquired growth is equal to or better than the growth acquired when microbial biomass is added to standard height fish meal and fish oil daily ration (basis+MB (microbial biomass)), just as shown in figs. 9 and 10.

embodiment 5

measure in clear water test and use microbial biomass to support to replace black Tiger Prawns Penaeus monodon completely fish meal in feed and the possibility of fish oil

The object of this experiment is not only the possibility detecting all fish meal content substituting prawn daily ration, is also to detect the possibility substituting fish oil.This comes from embodiment 4, namely replace fish meal and fish oil to show in Rinsing Area system completely effective, and we attempts in clear water system, to repeat this experiment to provide use industry the relevant further result arranged now.

The successful of this respect will show, microbial biomass can be used in offsetting and utilizes relevant performance losses with completely alternative any fishery resources.

method

Use is fished for net and is supported shrimp field from locality and catch 2,000 (2000) prawns, then these prawns are transferred in the polybag containing oxidation seawater, and transfer to subsequently in the storage facilities of laboratory, research center, Bu Laibi island (Bribie Island Research Centre laboratory), to observe for one to two week before distributing to clear water cultivating system.

Before starting experiment, kill 10 prawns representing each initial population, to carry out component analysis (3 sacks, each 5 prawns) and at being kept at-80 DEG C.

There is provided the tank of 30 × 2500L, described tank does not have matrix but has the filtering sea of BIRC sea water supply and single netted aeration.The water supply of all tanks circulates at first in the semi-closed system as a kind of storage cistern.Start precontract in experiment and within 2 weeks, set up tank to set up effective water bloom.Tank is set to simulate pond working condition as far as possible best.Before distributing to tank, from accumulator tank, random net is fished for 40 prawns and is weighed, to determine mean value and the standard deviation of population.Each tank is equipped with 30 prawns, and it has the mean value of 1 × SD based on each individuality.Collect 3 samples (each sample 5 prawns), pack and freezing, as three independent samples of initial population.

This experiment carries out 63 days.Again performance is checked when 21 days and 42 days.Carry out water coke slurry on request to keep water quality and water bloom.When needed, water coke slurry is generally 10% increment of overall water quality.Measure tank temperature every day and be recorded in 6 tanks wherein.As requested, by utilizing light-locking material control temperature, the highest acceptable temperature is 33 DEG C and minimum acceptable temperature is 25 DEG C.Temperature mainly stands physical alterations, and control temperature is only to attempt and remains in above-mentioned scope.

Measure other water quality parameters DO, temperature every day, pH, transparency (secchi depth) and salinity are then weekly that 1400 places of usage data record device (YSI) in all tanks are measured.Weekly from each tank water sampling, for nutrient analysis: measure TSS, ammonia, NOx and Chlorophyll.In 30 tanks, Random assignment 6 data logger are to record temperature change, count based on every day.

Feed used in this test is the repetition of embodiment 4.The formula prepared for this experiment is listed in fig. 8.

In experimentation, prawn feeding every day twice, in experimental session continuous 7 days one week.Each tank uses 2 delivery platies.All feeds are placed on delivery plate.The feed do not eaten removes (every morning and afternoon feed before first thing) from dish immediately and quantizes before nursing.Ration is determined by supply feed excessive a little.The quantity of food of record nursing every day.The food do not eaten removes and feeds subsequently from delivery plate.Estimate that the amount (number of pellet) of not eating food is to estimate consumption.

At the end of clear water experiment, from each tank, discharge waste water, catch prawn with net, immersed by ice and kill, and weigh separately.From each tank, collect 5 prawns be used for full shrimp body composition analysis.These prawns killed, weighs, shred, freeze and freeze drying, for subsequent analysis.The dry matter content of assessment sample when shredding.

result

Figure 11 and 12 shows, all fish meal in alternative prawn daily ration.But, compared with earlier results represented in Figure 10, when feeding prawn with the daily ration process not containing fish meal or fish oil in the place clear water system shown in Figure 12, observe the weightening finish not significantly loss of prawn.

Unexpectedly, the present inventor finds, is joined by microbial biomass in the daily ration process of raising prawn, not only contributes to any performance loss in weight discovered when the fish meal replaced in daily ration process and fish oil, and in fact result shows, the weight gain performance of prawn significantly improves.

These of the weight gain performance of prawn significantly improve the advantageous applications of proof microbial biomass composition in water biological species (such as prawn) growth.These microbial biomass compositions (see such as processing O2 to U2) are substantially free of the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal, such as fish meal and fish oil.

Therefore, can think, the present inventor confirms, and microbial biomass composition is used for the purposes of the fishery resources (such as fish meal and/or fish oil) in the basic daily ration process substituting water biological species (such as prawn).

Of the present invention various characteristic sum embodiments above described in each unitary part can optionally be carried out necessary amendment to it and be applied to other parts.Therefore, the feature that describes in detail of a part can optionally with the integrate features of other portion details.

All disclosures mentioned are in the above description included in herein by the mode quoted as proof.Any prior art of reference is not should not be considered as being that prior art forms admitting or any type of hint of a part for common practise in Australia yet in this manual.

Without departing from the scope of the invention, the various amendment of the method for the invention and product and change will be apparent to those skilled in the art.Although the present invention is described in conjunction with concrete preferred and embodiment and embodiment, it should be understood that the present invention for required protection should not be unduly limited in these specific embodiments.In fact, the apparent various modification for implementing described mode of the present invention should drop in following claim scope required for protection to those skilled in the art.

Claims

1. a feed product or feed ingredient, it comprises microbial biomass, wherein, described feed product or feed ingredient are substantially free of the resource that one or more are derived from fishery, described in be derived from fishery resource comprise the protein source being derived from aquatic animal and/or the lipid source being derived from aquatic animal.

2. the feed product of claim 1 or feed ingredient, wherein, described feed product or feed ingredient also comprise one or more lipid source being derived from plant and/or its mixture.

3. the feed product of claim 1 or claim 2 or feed ingredient, wherein, described in be derived from plant lipid source form about 1% to about 10%, preferred about 1% to about 5% or more preferably from about 2% to about 4% feed product.

4. the feed product of any one of claims 1 to 3 or feed ingredient, wherein, described microbial biomass forms about 0.5% to about 40%, about 0.5% to about 30%, about 0.5% to about 25%, preferably about 1% to about 20% or about 1% to about 15%, about 3% to about 12% or the feed product of about 3% to about 10%.

5. the feed product of any one of claim 2 to 4 or feed ingredient, wherein, described feed product or feed formula comprise microbial biomass and the combination of lipid source being derived from plant, wherein said feed product or feed formula comprise about 1% to about 10%, the preferably lipid source being derived from plant and about 0.5% to about 40% of about 1% to about 5% or more preferably from about 2% to about 4%, about 0.5% to about 30%, about 0.5% to about 25%, preferred about 1% to about 20%, or about 1% to about 15%, about 3% to about 12%, or the combination of the microbial biomass of about 3% to about 10%.

6. the feed product of any one of claim 2 to 5 or feed ingredient, wherein, the lipid source being derived from plant is selected from vegetable oil, soybean oil, mustard caul-fat or linseed oil and/or its mixture.

7. the feed product of any one of claim 2 to 6 or feed ingredient, wherein, the lipid source being derived from plant is linseed oil.

8. the feed product of any one of claim 1 to 7 or feed ingredient, wherein, described feed product is substantially free of the protein source that is derived from aquatic animal and/or is substantially free of the lipid source being derived from aquatic animal, wherein, the protein source being derived from aquatic animal is fish meal, krill meal or cuttlefish powder and/or its mixture, and the lipid source being derived from aquatic animal is fish oil, krill oil or cittle fish oil and/or its mixture.

9. the feed product of any one of claim 1 to 7 or feed ingredient, it also comprises the lipid source being derived from aquatic animal, preferred fish oil, krill oil or cittle fish oil and/or its mixture.

10. the feed product of claim 9 or feed ingredient, wherein, the lipid source being derived from aquatic animal forms about 0.1% to about 15%, about 1% to about 10%, about 1% to about 5% and about 1% to about 3.5% or the feed product of about 1% to about 2.5%.

The feed product of any one of 11. claims 1 to 7 or feed ingredient, it also comprises the protein source being derived from aquatic animal, preferably fish meal, cuttlefish powder or krill meal and/or its mixture.

The feed product of 12. claims 11 or feed ingredient, wherein, the protein source being derived from aquatic animal comprises about 0.1% to about 15%, about 1% to about 10%, about 1% to about 5% and about 1% to about 3.5% or the feed product of about 1% to about 2.5%.

The feed product of any one of 13. claims 1 to 12 or feed ingredient, wherein, microbial biomass is made up of the accessory substance or waste stream processing sugarcane generation, is preferably made up of bagasse, field trash, syrup, filter mud or other sugarcane waste products.

The feed product of any one of 14. claims 1 to 13 or feed ingredient, wherein, microbial biomass is made up of tapioca starch, bagasse, syrup or field trash.

The feed product of any one of 15. claims 1 to 14 or feed ingredient, wherein, microbial biomass is made up of bagasse and the lipid source being derived from plant is linseed oil.

The feed product of any one of 16. claims 1 to 15 or feed ingredient, wherein, microorganism comprises microorganism mixed population, described microorganism comprises micro-algae and bacterium, wherein with dry basis bacterium exist amount for about 5 % by weight to about 25 % by weight and micro-algae exist amount for about 0.1 % by weight to about 50 % by weight.

The feed product of any one of 17. claims 1 to 16 or feed ingredient, wherein, prepared by the total nitrogen that microbial biomass is about 40mg/L to about 70mg/L by initial concentration scope.

The feed product of any one of 18. claims 1 to 17 or feed ingredient are as the aquaculture feed of water biological species or the purposes of feed ingredient, and wherein, water biological species is preferably selected from fish, crustacean or mollusk.

19. 1 kinds of methods of raising water biological species, it comprises the feed product of any one to the claim 1 to 17 of water biological species member feeding effective dose or the step of feed ingredient.

20. 1 kinds of methods of raising water biological species, it comprises provides to water biological species the feed product or feed ingredient that are substantially free of fishery resources, wherein, described feed product or feed ingredient be claim 1 to 7 or 13 to 17 the feed product of any one or feed ingredient, wherein, water biological species is preferably selected from fish, crustacean or mollusk.

21. 1 kinds of small shrimps and prawn, its method by claim 19 or 20 obtains.

22. 1 kinds of methods preparing feed product or feed ingredient, described feed product or feed ingredient are substantially free of the water biological species of fishery resources, and described method comprises:

Microbial biomass as described herein is provided; And

One or more raw materials being selected from adhesive, Kohlenhydrate, the protein source being selected from the source except the protein source being derived from fishery, the lipid being selected from the source except the lipid being derived from fishery, vitamin, mineral matter and/or its mixture being suitable for expection water biological species of proportioning balanced in nutrition are added in microbial biomass.

The method preparing feed product or feed ingredient of 23. claims 22, wherein, described feed product or feed ingredient are used for prawn or small shrimp.