CN102016001A - Microbial biomass, feed product/ingredient and processes for production thereof - Google Patents
Microbial biomass, feed product/ingredient and processes for production thereof Download PDFInfo
- Publication number
- CN102016001A CN102016001A CN2009801157074A CN200980115707A CN102016001A CN 102016001 A CN102016001 A CN 102016001A CN 2009801157074 A CN2009801157074 A CN 2009801157074A CN 200980115707 A CN200980115707 A CN 200980115707A CN 102016001 A CN102016001 A CN 102016001A
- Authority
- CN
- China
- Prior art keywords
- microbial biomass
- water
- biomass
- bacterium
- aforementioned
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Insects & Arthropods (AREA)
- Sustainable Development (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
This invention provides a process for producing a microbial biomass comprising: a) providing a mixed population of microorganisms comprising micro algae and bacteria; b) adding a carbon source to the mixed population of microorganisms; c) adding a nitrogen source to the mixed population of microorganisms; d) culturing the mixed population of microorganisms under conditions suitable for the growth of both the microalgae and bacteria to form a microbial biomass; and e) harvesting the microbial biomass. This invention also provides a microbial biomass, a feed product or ingredient, uses of microbial biomass and feed product or ingredient and a method of rearing an aquatic species using the microbial biomass and feed product or ingredient of this invention.
Description
Technical field
The present invention relates to a kind of microbial biomass, feeds product or component that is used for water biological species, be used to produce the method for microbial biomass, be used to produce the method for feeds product or component, use described microbial biomass and described feeds product or component to culture the method for water biological species and the purposes of microbial biomass of the present invention and feeds product or component.Particularly, the present invention relates to microbial biomass, feeds product or component, be used to produce the method for microbial biomass and feeds product or component, use described microbial biomass and described feeds product or component to culture the method for water biological species and the purposes of described microbial biomass and described feeds product or component, described microbial biomass and described feeds product or component have utilized the microorganism that comprises little algae and bacterium to mix the group.
Background technology
Little algae is used as the feed of mollusc (molluscs), crustacean class (crustaceans) and some fish in aquaculture, and is used for the feed of the zooplankton that the aquaculture food chain uses.Therefore, in Australian environment, little algae Pacific Ocean Margarita, prawn, barramunda (barramundi) and childhood abalone and the larva of other emerging species produce and play a key effect.For many years, tested the possibility of hundreds of little algaes, but may only obtain success and be used widely less than 20 kinds as food.Little algae must have some key characteristics could be as the available aquaculture species.They must have the size (being the 1-15 micron for filter feeder for example) that is fit to be ingested and must be digested easily.They must have the fast speed of growth, can cultivate in a large number, and must keep stable under the situation of contingent any temperature, illumination and nutrient substance fluctuation in culture systems in cultivation.At last, they must have good trophic component, comprise not containing the toxin that can transmit in food chain.
The nutritive value of various little algae kinds can be obviously different, and nutritive value can change with different culture condition.Yet, can be the animal larva through careful little algae mixture of selecting fabulous nutritive cube directly or indirectly be provided (by the enrichment of zooplankton).Be found and had good nutrition character---with single species or in mixed diet---little algae comprise calcareous Chaetoceros (C.calcitrans), Chaetoceros muelleri (C.muetteri), Lu Zipafu algae (P.lutheri), Isochrysis galbana belong to kind of (Isochrysis sp.) (T.ISO), inferior heart-shaped flat algae (T.suecica), middle Skeletonemacostatum (S.costatum) and false miniature hailian seaweed (Thalassiosira pseudonana).
Usually, little algae can provide proteic abundant source, and has isostatic amino acid composition.Although total composition of little algae can influence nutritive value, nutritive value is the balance that possible have other critical nutrients of maximum effect.Polyunsaturated fatty acid (PUFA) is docosahexenoic acid (DHA), timnodonic acid (EPA) and arachidonic acid (AA) especially---known they essential by multiple larva---has remarkable difference between different guiding principles and algae not of the same race.Although to the EPA of high density, seldom have species to be rich in DHA during most of species have.It is the example that is rich in little algae of DHA that Isochrysis galbana belongs to the latent algae (Rhodomonas salina) of kind of (T.ISO), a Lu Zipafu algae (Pavlova lutheri), little little unit cell algae (Micromonas pusilla) and sabkha.
Be used for a large amount of indoor typical systemss of cultivating of little algae and comprise thin mouthful of carboy (10-20L), polyethylene bag (100-500L) and bucket (1000-5000L).These systems operate in mode in batches or continuously usually.For bigger volume, use the outside water box or the pond of operating in semicontinuous mode.According to its scale, the hatcher can produce hundreds of every day and rise algae to several ten thousand.For these modular systems, cell density is every milliliter 10
5-10
7Individual cell, production cost can be the 20-50% of 50-200 dollar or hatcher operational cost.Have tangible scale economics for algae production, so production cost becomes especially big to less hatcher.Therefore, a lot of effort have been carried out to obtain to have more cost-benefit production system.
One of known difficulty of large volume outdoor system is that they suffer microbial contamination easily.Though can obtain the single culture of planting of little algae, this needs strict culture environment, for example high salinity and/or high basicity.By contrast, too expensive though indoor closed system can realize single algae culture of planting as little algae source of culture fishery, thereby be more suitable for high value applications, for example medicine is produced.
Therefore, need be used to produce improved, economic, the large-scale methods that to gather in the crops as little algal biomass of aquaculture feed.
The applicant finds, promotes that at the same time culturing micro-organisms mictium can produce the microbial floc that improves as the character of used for aquiculture feed under the condition of growth of little algae and bacterium.For example, the shrimp of feeding with the diet that is added with described microbial floc is compared with lobster with the shrimp of feeding with regular diet with lobster and shows the speed of growth faster.Produce the method for the little algal biomass of used for aquiculture with being used to before and compare, by adding the growth that can in fact have been promoted bacterium by the carbon source that bacterium utilizes.
Therefore, the microbial floc of generation contains the biomass of a large amount of bacterial origins.In the past, bacterium can be counted as pollutent.
Summary of the invention
Therefore, the invention provides a kind of method that is used to produce microbial biomass, comprising:
A) provide the microorganism that comprises little algae and bacterium to mix the group;
B) in described microorganism mixing group, add carbon source;
C) in described microorganism mixing group, add nitrogenous source;
D) be suitable for cultivating under the condition of described little algae and bacterial growth described microorganism at the same time and mix the group to form microbial biomass; And
E) gather in the crops described microbial biomass.
The applicant has been found that when the carbon in the substratum: the nitrogen ratio is between 6: 1 and 18: 1 the time, and the output of described microbial floc increases.Described microorganism mixes the group also can comprise little algae, yeast, fungi, protobiont, microplankton and bacterium.
In one embodiment, described carbon source is the low value agricultural waste, and it can further save cost in the production of described microbial floc.
Described cultivation can for example be carried out in water tank, pond or the ditch in the open system different with closed system biological example reactor.In the past, these open systems were considered to not to be to obtain to be used for the desirable means of little algae that aquaculture and other should the way owing to possible bacterial contamination.Yet because the basis of methods described herein is also to promote bacterial growth except promoting micro algae growth, therefore so-called bacterium " pollution " no longer is a problem.
In the method for the invention, described culturing step d) can in open container, carry out.Particularly, described culturing step d) can in the open system that includes but not limited to water tank, ditch or pond, carry out.
When cultivating described microbial biomass in the pond, described pond can add lining with one or more polymer sheet.Described polymer sheet can be included but not limited to that high density polyethylene(HDPE) (HDPE) makes by suitable material.Yet described pond also can partly add lining with polymer sheet along edge and dike (bank).Described pond also can not lining.
When cultivating described microbial biomass in the ditch, described ditch can be given with one or more polymer sheet and add lining.Described polymer sheet can be included but not limited to that high density polyethylene(HDPE) (HDPE) makes by suitable material.Described ditch also can be positioned at for it and keep out wind and rain but the structure that allows sunlight to penetrate fully.
When cultivating described microbial biomass in water tank, described water tank can be positioned at for it and keep out wind and rain but the structure that allows sunlight to penetrate fully.The preferred depth of water can be 0.5-3m, 1-2.5m, 1-1.5m, 1-1.25m or about 1m in the described culture systems.Yet described biomass also can be incubated in the more shallow or darker water.
In the method for the invention, described culturing step d) can in saltwater environment, carry out.Described culturing step d) can in the saltwater environment of the effluent brine that includes but not limited to seawater, desalimtor, landlocked salt solution etc., carry out.As described culturing step d) when carrying out in seawater, described salt solution can be the seawater that salinity is 5-60ppt, 10-50ppt, 15-40ppt, 20-35ppt or 30-35ppt.Yet this should not be limited in the step of described cultivation biomass within these salinity, because also can cultivate in having water lower or more high salinity.
The existence that method of the present invention also is included in described carbon source and nitrogenous source will comprise that down the microorganism of little algae and bacterium mixes the violent step of mixing with the flocculation particle suspension that keeps described microbial biomass of group.The flocculation particle (microbial floc) of the microbial biomass that suspends also can comprise the mixture of little algae, bacterium, yeast and fungi, organic debris material, protobiont and other microorganisms.
Method of the present invention can comprise that also the biomass drying that will gather in the crops is to form the step of feeds product or feed ingredient.
Used in the present invention carbon source can be the carbon source of being utilized by the bacterium in the inventive method.In one aspect of the invention, whole or most described carbon source can be utilized by described bacterium.Described carbon source also can be utilized by described bacterium and/or little algae.Described carbon source can be selected from depleted large volume, low value agricultural material and agricultural waste.Described low value agricultural material can comprise from sugarcane (comprising molasses), rice, wheat, triticale, corn, Chinese sorghum, cassava, oleaginous seed (oilseed) (comprising the Semen Brassicae campestris dregs of rice (canola meal) and feather fan beanpod (lupinhull)) are carried out product processed, byproduct or waste streams (waste stream), and the silo dust of cereal source mill (elevator dust).Other carbon nitrogen sources can comprise the processing waste and the distillery exhausted grain products of feed-processing plant.Carbon source of the present invention also can be the material that grinds or sieve.Can be the shell of lupine, Semen Brassicae campestris (canola), peanut or other oleaginous seeds in carbon source described in the specific examples, described shell has been ground or has been sized to, and can to make these particles be the small particle size of the sieve of 2mm by screen size.
Described nitrogenous source can be any economically with environment on suitable product, for example urea, ammonia, ammonium nitrate, ammonium phosphate chemical fertilizer, and the organic nitrogen source that comprises the waste water that aquaculture pond is discharged.Also other nitrogenous sources can be added in the described culture systems.When cultivating beginning in the described culture systems total nitrogen concentration can be 10-30mg/L, perhaps be about 20mg/L.
A second aspect of the present invention provides by the method for the present invention microbial biomass produced of a first aspect of the present invention particularly.
A third aspect of the present invention provides and comprises that microorganism mixes group's feeds product, and described microorganism mixes the group and comprises little algae and bacterium, and wherein bacterium is about 20: 1 to about 0.4: 1 with the dry weight ratio of little algae.To described little algae carry out quantitatively can be based on the content of the contained chlorophyll a of described microbial biomass, and to described bacterium carry out quantitatively can be based on the content of teichoic acid.
Another aspect of the present invention provides and comprises that microorganism mixes group's feeds product or feed ingredient, described microorganism mixes the group and comprises little algae and bacterium, wherein said bacterium exists to the amount of about 25wt% in the about 5wt% of dry-matter, and little algae exists to the amount of about 80wt% in dry-matter 10wt%.Described feeds product or feed ingredient also can comprise the bacterium to the amount of about 20wt% in the about 5wt% of dry-matter.Described feeds product or feed ingredient can comprise the microbial biomass of the present invention of 5-20wt% at least.
When described microbial biomass as feed ingredient when forming feeds product, can be with described microbial biomass and tackiness agent (wheat bran for example, alginate or starch), other protein source (fish meal for example, the cuttlefish powder, krill meal, soyflour, the feather fan bean powder, the wheat bran powder), be rich in the especially component of rich in starch (wheat-flour for example of carbohydrate, rice bran, Tapioca Starch, ground rice, Semen Maydis powder), lipid source (fish oil for example, cittle fish oil, krill oil, vegetables oil, soybean oil, Canola oil, soybean lecithin), be suitable for the vitamine mixture of purpose species, be suitable for mineral mixture and other nutritional additives of purpose species.
A fourth aspect of the present invention provides its amount that the biomass of the present invention of nutrient substance or the purposes of feeds product of the present invention can be provided effectively to a kind of animal of water biological species.
A fifth aspect of the present invention provides a kind of method of culturing water biological species, comprises that described amount can provide nutrient substance effectively to described water biological species to a kind of animal of water biological species the feed biomass of the present invention of following amount or the step of feeds product of the present invention.
Described water biological species can be selected from fish, crustacean class and mollusc.Described fish are optional from Atlantic Ocean salmon (Altantic Salmon), barramunda and cabio (cobiatrout).Described crustacean class can be selected from shrimp, lobster and crab.Described mollusc can be selected from oyster, scallop and abalone.
The present invention is from following discovery: carbon source is cultivated and is carried out the product that bio-transformation derives and can produce useful food, feed ingredient and the bioactive compounds of aquaculture species and other domestic animals through the many species of non-pure property of water microorganisms.
Method of the present invention also can may further comprise the steps: the carbon-nitrogen ratio (C: N ratio) of optimizing nutrient substance level and carbon source that is easy to utilize and nitrogen in the production of described microbial biomass.
The nutrient substance that can be included in the step of optimizing the nutrient substance level is selected from phosphoric acid salt, silicate and their mixture.Described phosphoric acid salt can be selected from KH
2PO
4, superphosphate, heavy superphosphate, triple superphosphate, primary ammonium phosphate, Secondary ammonium phosphate, rock phosphate and Agras.
The silicate concentration of described culture systems can be adjusted to the silicate of 20mg/L-163mg/L.The ratio of silica and nitrogen can be not less than 1.5: 1.
KH
2PO
4Can be according to the P that 2: 1 to 20: 1,3: 1 to 18: 1,4: 1 to 16: 1,4: 1 to 14: 1 or 5: 1 to 10: 1 are provided: the amount of N ratio adds.Other P: the N ratio can be 5.1: 1,5.2: 1,5.3: 1,5.4: 1,5.5: 1,5.6: 1,5.7: 1,5.8: 1,5.9: 1,6.0: 1.Phosphorus concentration can exist pro rata with nitrogen content in the water of described culture systems.P: the N ratio can be about 5.8: 1 (P: N).Can contain for example primary ammonium phosphate phosphate concn increase level so far that makes described cultivation water of phosphatic chemical fertilizer by adding.The phosphate concn of described culture systems water can be adjusted to 90mg/L to being less than 710mg/L.
Described silicate can be selected from water glass, Starso (Na2SiO
3.5H
2O), water glass and potassium silicate.
Described silicic acid source can be according to the Si that provides 1: 1 to 5: 1,1: 1 to 4.5: 1,1: 1 to 4.0: 1,1: 1 to 3.5: 1,1: 1 to 3.0: 1,1: 1 to 2.5: 1,1: 1 to 2.0: 1,1: 1 to 1.5: 1: the amount of N ratio adds.
Optimize the gentle C of nutrient water in the methods of the invention: the step of N ratio also can comprise and is low to moderate 5mg L
-1Nitrogenous source level and any available carbon source, comprise any wastes of crops.Described C: the N ratio can be 2: 1 to 24: 1,3: 1 to 20: 1,4: 1 to 18: 1,5: 1 to 18: 1 or 6: 1 to 18: 1.Other C: the N ratio can be 10: 1,11: 1,12: 1,13: 1,14: 1,15: 1,16: 1,17: 1,18: 1,19: 1,20: 1,21: 1,22: 1,23: 1 and 24: 1.C of the present invention: some specific examples of N ratio comprises 6: 1,12: 1 and 18: 1.We also find, when the carbon in the substratum: nitrogen than between the specified proportion when (6: 1 to 18: 1) output of described microbial floc increase.
Method of the present invention can comprise that also be the step of the efficient of useful food or feed ingredient by grinding and sieve surface-area and the volume ratio of optimizing described carbon source to improve bio-transformation.
Product of the inventive method and feed of the present invention comprise the output of every 2.4t water tank 0.1kg to the dried microbial biomass of every 2.4t water tank 1.1kg, produce the dried microbial biomass and the microbial proteinous (referring to embodiment 2) of maximum amount from smart (＜710 μ m) organic carbon material.
Wet microbial biomass can for example drying be carried out in dry air or lyophilize by suitable means.The water-content of described dried microbial biomass is reduced to be lower than 15% or be lower than 10%.Can and use the standard method of analysis analysis with the dry biomass samples weighing from each culture systems, gathered in the crops to measure dry-matter, crude protein, TL and ash content.
Described dried microbial biomass can described component mixture 5-15% or the level of 5-10% be included in the feed formulation.Described dried microbial biomass can use according to the mode identical with any other dry feed component.
The preferable use of described biomass can at first be as animal feedstuff additive; Secondly as the hydrocoles fodder additives; Once more as the fodder additivess of mollusc, crustacean class and fish.
The source of microorganism can comprise one or more microorganisms that exist or Lock-in in the used water of starter culture or culture systems in described culturing step.Any microflora that described culturing step can utilize the water that is used for filling with described pond, ditch or water tank to exist.This group can become the microorganism starter culture that is used for the inventive method.The water that is used to fill with described cultivation pond, ditch or water tank can be natural, the unfiltered seawater of taking from any water source, and described water source includes but not limited to the waste water or the recycle-water of preceding culture behind the described microbial floc of results of ocean water or rivers water, aquaculture pond.
Microorganism starter culture also can be added the natural population with little algae and/or bacterial cultures.Other starter cultures can be added in the entry to replenish existing microflora at described culturing step, described other starter cultures comprise the water of taking from the source of being rich in little algae and bacterium.Also in the mixing microorganisms group that the little algae or the bacterium of a particular cluster can be added described culture systems to quicken or to improve matter or the amount that microbial biomass is produced.Can be by carrying out the growth that other step is suppressed at undesired little algae kind (for example blue-green algae) in the described culture systems in the method for the invention.These other steps are included in the described culture systems and change ventilation levels, discharge the surface water that contains undesired little algae, change pH, basicity or phosphoric acid salt or silicate concentration.
When adding little algae culture, described little algae can be diatom.When adding bacterial cultures, described culture can be nitrobacteria.
The water source that is used to fill with described culture systems can contain high-caliber nutrient substance, and especially ammonia, nitrate, nitrite and organic compounds containing nitrogen are such as but not limited to the nitrogen of albumen and amino acid form.
Described culturing step in the inventive method can comprise that stirring suspends to keep particulate matter (comprising throw out).Stirring water can ventilate to it, guarantees not form anoxic zones in the bottom of described culture systems.Described stirring can realize that described means comprise the aerator that for example is selected from vane type aerator, air spray aerator, pneumatic plant and gas lift equipment by suitable means.
Method of the present invention can comprise that also the pH with described culture systems remains on 7.3-8.3.Described pH can use suitable alkaline matter to improve, and described alkaline matter is such as but not limited to the material that is selected from white lime, Aglime, rhombspar and yellow soda ash.
The step of cultivating described microbial biomass can carry out decomposing and being converted into described flocculant biological matter until most of carbon source continuously.This can determine by the microscopy to biomass.According to producing economy, used carbon source and the actual conditions of cultivating, the time that described culturing step carries out can be for about 4 to about 8 weeks,
The results step of the inventive method can comprise makes the inclusion of described culture systems pass through filter plant.Described filter plant can be sieve formula strainer (screen filter), baleen strainer (Baleen filter) for example, and perhaps continuous flow centrifugation equipment is to concentrate described microbial biomass.Can make by described strainer or by the liquid return of described whizzer and think that to cultivating in pond, ditch or the water tank next batch microbial biomass provides water, some nutrient substance and starter culture.
Can push the biomass gathered in the crops to remove more maintenance water, rapid drying then.Drying can be carried out under high gas flow and neutral temperature fast.Described temperature can be 40-80 ℃.Perhaps, can be 40 ℃ of high gas flow and lesser tempss dry down or can be at 12 hours inner dryings, take place or do not decompose.The exsiccant product can contain and be less than 10% moisture.
Described microbial biomass also can comprise the minimum bacteria content that accounts for dry biomass 5%.
Embodiment
Embodiment
Embodiment 1(cultivation of microbial biomass)
For the indication of the optimum level of acquisition nitrogen and the carbon source that is easy to utilize in microbial biomass production, carried out first experiment.Described experiment is made of 12 processing, and wherein every group all comprises two same water tanks.Described processing is included in 4 nitrogen (N) concentration (table 1) in 12 water tanks.Described nitrogenous source is a urea fertilizer.The organic carbon of tapioca (flour) form is joined in two same water tanks so that C with the N of each concentration: the N ratio is 6: 1,12: 1 and 18: 1 (table 1).The water tank that is used for cultivating described microbial biomass is to be positioned to make the available light decay minimum and make water temperature remain on the circular glass fiber water tank (2450L working volume) of 25-33 ℃ chamber, gardening tunnel (horticulturaltunnel house).
(20 μ m) fills with described water tank with filtering seawater, begins described experiment after 5 weeks.By in each water tank, adding 20L from Cleveland Point, Moreton Bay, the not filtering sea that Queensland gathers and add other microorganism.Make water in the described cistern system be circulated throughout mixing tank and be pumped back to that thereby to make water in the described water tank be simple substance basically.Promote little algae wawter bloom (bloom) by in each water tank, adding a spot of nutrient substance: 0.53g urea, 0.1843g KH
2PO
4With 2.8g Na
2SiO
3.5H
2O.In case the realization wawter bloom just stops described circulation, and carry out specified urea, tapioca (flour) processing.Simultaneously, in all water tanks, add the phosphoric acid salt of equivalent (with KH
2PO
4Form) and silicate (Na
2SiO
3.5H
2O).In described water tank, add KH
2PO
4So that 5.8: 1 P to be provided: the N ratio, and add Na
2SiO
3.5H
2O is to provide 1.5: 1 Si: the N ratio.
Make nutrient substance and particulate matter in the described water tank keep mixing and suspending by fierce ventilation and use gas lift equipment.After 37 days, the described microbial biomass of disposable results from a water tank.This operated 3 days consuming time.At first close ventilation, make described microbial biomass and particulate matter precipitation.After 1 hour, with water by near the filter bag sucking-off of surface until arriving sedimentary biomass.Make the remaining water of large number of biological matter that contains by described filter bag then.Slow when unacceptable when the speed of water by filter bag, twist described filter bag and filtering biomass are transferred in the plastics bag, freezing immediately then.Continuing the whole water of this process in described water tank all is filtered.
The microbial biomass that will wet is weighed, and spreads out on the stainless steel pallet, and places 2 days in 40 ℃ high gas flow drying oven.The water-content of described dried microbial biomass is reduced to be lower than 10%.The dry biomass of gathering in the crops from each water tank is weighed and used standard method of analysis to analyze to measure dry-matter, crude protein, TL and ash oontent (table 2).The result shows that the output of biomass improves along with the increase of N adding, but is incorporated as 20mg L at N
-1The time remove efficient (retention efficiency) the best.As if 12: 1 C: N is than the optimum efficiency that provides described biomass to produce, although 18: 1 C: the N ratio is tending towards producing more albumen.
Table 1. is for the processing of the 2450L water tank that is used for the culturing micro-organisms biomass and add urea, tapioca (flour), KH
2PO
4And Na
2SiO
3.5H
2The amount of O
The composition of the dried microbial biomass of the output of each water tank and cultivation among table 2. embodiment 1.Crude protein, TL and ash oontent in dry-matter.
Embodiment 2(cultivation of the microbial biomass that use feather fan beanpod carries out)
The purposes of the low-cost carbon source of cultivating as microbial biomass for assessment feather fan beanpod and carry out this embodiment for the output of relatively using feather fan beanpod and tapioca (flour) to obtain.Described feather fan beanpod is that the processing lupin seed is to obtain the by product of feather fan beans.Also therefore provide bigger surface-area and volume ratio by the hammer runner milling with the size that reduces them with feather fan beanpod sample.Estimate that this can accelerate microflora in the described cultivation water tank to the bio-transformation speed of feather fan beanpod material.As if the ground material comprises two kinds of wide particle diameter fractions, sieves to separate them with the sieve of 710 μ m.Described two kinds of fractions provide two kinds of carbon sources.The analysis of described fraction has been shown nuance (table 3) in the similar composition.Refining lupine fiber is included as other processing, and it is a product a kind of commercial preparation and be used as fibre additive by foodstuffs industry.
Described experiment comprises the processing based on 6 kinds of carbon sources, handles two identical water tanks of distribution to every kind in the design of a completely random.Described being treated to: (A) contrast does not add carbon source; (B) Tapioca Starch; (C) refining lupine fiber; (D) thin feather fan beanpod material; (E) thick feather fan beanpod material; (F) ground feather fan beanpod not.The water tank that is used to cultivate described microbial biomass is the circular glass fiber water tank (volume of water of 2450L) that is positioned at chamber, gardening tunnel one side-lower.Chamber, described tunnel can make available light decay minimum and can make water temperature remain on 25-33 ℃.Fill with each of described water tank with not filtering sea (20L) and filtering sea water mixing thing, begin described experiment after 4 weeks.Add nutrient substance and cultivate (each water tank 0.53g urea and 0.1843g KH with the bottle opener of beginning microorganism
2PO
4).
Thereby being circulated throughout, described water guarantees in mixing tank and the described water tank of blowback that the microflora in all water tanks is identical with nutrient concentration before the experiment beginning.In case the realization wawter bloom just stops described circulation, and each water tank is carried out specified urea and carbon source handle.
Add urea 20mg is provided L
-1N (every water tank 105g urea), and add carbon source so that 12: 1 C to be provided: N is than (every water tank 1.323kg).In addition, in whole water tanks, add KH
2PO
4So that P: the N ratio is 5.8: 1 (every water tank 509g).Make nutrient substance and particulate matter in the described water tank keep mixing and suspending by fierce ventilation in each water tank and use gas lift equipment.
After 39 days, the described microbial biomass of disposable results from a water tank.This operated 2 days consuming time.At first close ventilation, make described microbial biomass and particulate matter precipitation.After 1 hour, with water by near the filter bag sucking-off of surface until arriving sedimentary biomass.Make the remaining water of large number of biological matter that contains by described filter bag then.Slow when unacceptable when the speed of water by filter bag, twist described filter bag and filtering biomass are transferred in the plastics bag, freezing immediately then.Continuing the whole water of this process in described water tank all is filtered.The microbial biomass that will wet is weighed, and spreads out on the stainless steel pallet, and places 40 ℃ high gas flow drying oven 2 days.
The water-content of described dried microbial biomass is reduced to be lower than 10%.The dry biomass sample of gathering in the crops from each water tank is weighed and used standard method of analysis (AOAC 1991) to analyze to measure dry-matter, crude protein, TL and ash oontent (table 4).
The result shows that the maximum concentration of crude protein appears at and uses Tapioca Starch to carry out in the cultured microorganism biomass as carbon source.Yet this amount of handling the biomass that produce is minimum.
The output of humidogene material is different, and wherein the dried microbial biomass of maximum is to produce in the water tank that contains thin lupine (＜710 μ m) material.On an average, the output that obtains of this experiment is about 1kg/2.4 ton water tank.Described experiment shows that the feather fan beanpod is a kind of effective carbon source of culturing micro-organisms biomass, and the fine grinding beanpod has improved the output of microbial proteinous.
Table 3. is used for the composition of the carbon source of microbial biomass cultivation.Unless otherwise, the result is in DM%.ADF=acid detergent fiber (acid detergent fiber)
*% presses sample and calculates (as received)
Table 4. use cassava and feather fan beanpod are as the output (air dried biomass) and the composition of carbon source cultured microorganism biomass.The result is from two identical water tanks.The composition data are expressed as the % in dry-matter.The ADF=acid detergent fiber
Embodiment 3.
The target of this research is that research is used for deceiving Tiger Prawns (black tigershrimp) when joining---the difference of the nutritive value of multiple microbial biomass culture during the feed of tigar prawn (Penaeus monodon).Described microbial biomass is the general introduction according to embodiment 1 and 2, produces in the 2500L water tank with CSIRO equipment at Cleveland.This research is carried out in the system of clear water aquarium.
Described experiment comprises with basal diet and all contains 100g kg
-135 days feeding experiments carrying out of a series of 15 kinds of diet of the dried microbial biomass of cultivating batch from difference.The prescription of described basal diet and three kinds of test diets is shown in the mode of table 1 with the described diet of explanation preparation.
In process for preparation, crude protein content and lipid content (75g kg
-1DM) in all diet, all be maintained on the par and (be respectively 420g kg
-1With 75g kg
-1DM).Comprise par (100g kg in the described test diet
-1) described dried microbial biomass, and the amount of regulating casein, vegetable oil mixt and wheat starch is with the described prescription of balance.Composition after weighing is thoroughly stirred in planetary stirring machine, add about 40% the water that volume equals the component dry weight then, continue to stir to form crisp dough/pasta (crumbly dough).Described dough/pasta is pressed through Hobart A-200 stirrer (Hobart Corporation, Troy, OH, mincer annex USA).With the band (the about 3mm of diameter) of the spaghetti strip that squeezes out at normal pressure steamer (Curtin; Son, Sydney, Australia) in boiling 5 minutes, in the forced ventilation cupboard and 40 ℃ of following air-dry overnight and be divided into the long segment (pellet) of 5-8mm.Described segment is kept under-20 ℃ until use.
The tigar prawn larva is to obtain from the commercial shrimp aquaculture field in Queensland, Australia the north.They are remained in about 1 week in the 2500L water tank of CSIRO oceanographic research laboratory of Cleveland, be transferred to the less water tank that is used for described experiment then.In the time of in remaining in described 2500L water tank, every day is with commercially available tigar prawn feed (CP#4004, CP Feeds, Samut Sakorn, Thailand) feed twice of described shrimp.Described water tank is filled to remain on 28 ± 0.5 ℃ of mobile, filtered seawater (salinity 32 to 36 ‰) under the temperature.For described growth response experiment, use the array of circular white polyethylene indoor water tank (120L volume, 600mm diameter).Each water tank is filled the min with 600mL
-1Speed mobile filtered (10 μ m) and the seawater of heating so that water tank temperature remains on 29 ± 0.5 ℃, and carry out supplemental inflation with single isolated bubbles stone.Monitor water temperature every day and in whole experiment, keep illumination in 12 hours: 12 hours dark photoperiods.
Before experiment beginning, each is weighed and size classification does not make the shrimp in the rank have the weight range that is no more than 0.5g with described shrimp.Shrimp between 2.5g and the 3.6g is used for this experiment.Described shrimp is dispersed in the water tank array, and 6 shrimps of each water tank make that the biomass in all water tanks are all similar.
Make described shrimp adapt to described water tank condition and basal diet 7 days, each is weighed once more when described experiment begins then.When this is weighed, in each water tank, only put back to variability (mean value ± standard deviation=3.2 ± 0.30g) of 5 shrimps with the weight range of each shrimp and biomass in the further reduction water tank.
When described experiment finishes after 25 days and at the 35th day, described shrimp is weighed once more.In described experimentation, give every day described shrimp feed its specify feed through weight quota twice, usually at 08:30 and 17:00.Clean described water tank every afternoon and use the grade of 0-4 to write down the amount of the feed of not eating up in the described water tank.
According to the feed quota that this value is regulated next day, purpose is to make the amount of the feed of not eating up minimum and guarantee that simultaneously growth is by the not enough restriction of successive feeding.Any death or missing shrimp shrimp that has mark with similar size within 24 hours are replaced.The replacement shrimp that has mark is used for keeping the constant cultivation density of described water tank but is not included in the data that are used for analyzing growth response or survival.Although the weight of each shrimp all goes on record, have only the weight in average of unlabelled shrimp in each water tank to be used for data analysis.
Experimental result is shown in table 2.Survival rate is all higher in great majority are handled, and the mean value of described experiment is 85%.For 11 kinds in 15 kinds of diet, in described feed, add the phenomenal growth that microbial biomass has caused growth.Under any circumstance with containing growth that the microbial biomass diet obtains numerically not as the situation of the growth that obtains with basal diet.Average increment by adding the growth that growth that microbial biomass obtains obtains with basal diet in described feed is 35% (sd=10.5%).
See the following form 1.The basal diet of described diet manner of formulation and the component composition of three kinds of test diets are described.
Table 1
The feed biological respinse parameter of shrimp in described 4 weeks of test diet of table 2..Income is with the difference between the growth of described test diet and basal diet acquisition, is expressed as the percentage ratio of basal diet.The initial weight of shrimp (mean value ± standard deviation=3.2 ± 0.3g)
Embodiment 4
The target of this research be measure when black Tiger Prawns (black tiger shrimp) tigar prawn (Penaeus monodon) when the clear water culture environment is grown, the effect of the dried microbial biomass of adding in the feed of shrimp.
Described experiment relates to the processing based on four kinds of diet, handles for every kind and distributes 6 identical 2500L water tanks.Described diet comprises that basal diet and two kinds contain 50 and 100g kg respectively
-1The diet of dried microbial biomass level.Described diet comprises basic thing (900g kg
-1)---its be commercially available shrimp feed (starter diet, Ridley AquaFeeds, Narangbar, QId, Australia) with remainder (100g kg
-1) undressed feed ingredient mixture, described remainder comprises the mixture of casein, lupine chaff and ground clam shell (ground clam shell), and/or dried microbial biomass (referring to table 1).
Component after weighing is thoroughly stirred in planetary stirring machine, add about 40% the water that volume equals the component dry weight then, continue to stir to form crisp dough/pasta.Described dough/pasta is pressed through Hobart A-200 stirrer (Hobart Corporation, Troy, OH, mincer annex USA).With the band (the about 3mm of diameter) of the spaghetti strip that squeezes out at normal pressure steamer (Curtin; Son, Sydney, Australia) in boiling 5 minutes, in the forced ventilation cupboard and 40 ℃ of following air-dry overnight and be divided into the long segment of 5-8mm.Described segment is kept under-20 ℃ until use.
24 2500L glass fibre water tanks are placed in the chamber, gardening tunnel.Fill with seawater to described water tank, do not contain the sand substrate in the bottom, and each all is equipped with netted inflatable substrate (aeration).The water supply of all water tanks circulates in semi-enclosed system as the water of one.By in remaining on the water tank water temperature among a small circle, guaranteeing that minimum temperature is 27.5 ℃ with heat exchanger heats water.When needing under the fine day in sweltering heat, on chamber, whole tunnel, cover the gobo ceiling and rise above 33 ℃ to prevent the water temperature.
Described experiment begins preceding 2 weeks, will be from Cleveland Point, and Moreton Bay, the 20L that Queensland gathers not filtering sea adds in each of described water tank, and the filtered seawater of supplying with the laboratory is filled with described water tank again.Apply fertilizer to realize little algae wawter bloom at described water tank mild or moderate with urea then.In whole experiment, follow shrimp pond management, optionally by emitting and replacing water is regulated little algae wawter bloom and nutrient substance level in the water.
(Seafarm, Cardwell Qld) gather the shrimp that surpasses 2000 3.5-6.0g from the commercial shrimp aquaculture field at Queensland middle part.Described shrimp weighed in described shrimp aquaculture field and place the delivery case, described delivery case is assigned to concrete water tank.75 shrimps of each water tank distribution make that size is formed all similar and significantly different (mean value ± standard deviation=3.2 ± 0.3g) of nothing of weight of peace between water tank.Behind the CSERO facility that arrives the Cleveland, described shrimp is directly put into specified water tank, the experiment beginning.
Specify feed feed described shrimp 3 time (usually at 06:00,11:00 and 17:00) with it every day.In each water tank, all feed all is placed on two pallets of feeding (diameter 300mm).Before being about to feed, take out the described pallet of feeding next time, and assessment feed residual content.Judge according to remaining feed on the pallet of feeding, described shrimp is fed the weight ration to full.After 4 weeks, measure growth, FCR and survival rate at each water tank of emptying and after obtaining whole shrimps.Measure DO, temperature, pH, opacity, salinity in whole water tanks at 05:00 and 14:00 by usage data record device (YSI) and monitor twice of water quality in whole water tanks every day.Also gather water sample weekly and be used for nutrient substance analysis (ammonium, dissolved nitrogen, nitrate).Measure fluorescence (chlorophyll) and illumination decay in addition.
The results are shown in table 2.The very fast and obviously quickening of the shrimp speed of growth of described shrimp along with the increase of the dried microbial biomass that adds.The foodstuff picked-up also increases along with the increase of dried microbial biomass level, so FCR keeps relative stability in treating processes.Survival rate in all treating processess is all very high, and average survival rate is 93%.Described result clearly illustrates that the quickening of the speed of growth is attributable to add dried microbial biomass in described feed.
The component of table 1. test diet and nutrition are formed (g kg
-1).
The feed initial weight and the biological respinse parameter of shrimp in described 4 weeks of test diet of table 2..
Advantage
Advantages more of the present invention are as follows:
1) in clear water or clear water, cultures and about 35% (sd=10.5%) (referring to embodiment 3 and 4 above) accelerated in growth that the speed of growth of the shrimp of the diet that contains microbial biomass of feeding obtains with the basal diet that contains wild results fish meal.
2) size of described shrimp is bigger in when results, and make the price of per kilogram be higher than the equivalent biomass than shrimp (being up to more than 25%).
Conspicuous for a person skilled in the art improvement and modification are considered within the scope of the invention.The present invention should be understood and above-mentioned specific embodiment and embodiment should be restricted to.
Claims (29)
1. method that is used to produce microbial biomass comprises:
A) provide the microorganism that comprises little algae and bacterium to mix the group;
B) in described microorganism mixing group, add carbon source;
C) in described microorganism mixing group, add nitrogenous source;
D) cultivate described microorganism and mix group, described carbon source and described nitrogenous source to form described microbial biomass; And
E) gather in the crops described microbial biomass.
2. the process of claim 1 wherein described culturing step d) in open container, carry out.
3. claim 1 or 2 method, the biomass that also comprise dry results are to form the step of feeds product.
4. the method for claim 2, wherein said culturing step d) in water tank, ditch or pond, carry out.
5. each method of aforementioned claim, wherein said carbon source is selected from processes product, byproduct or the waste streams that obtains to sugarcane, molasses, rice, wheat, triticale, corn, Chinese sorghum, cassava, oleaginous seed, the Semen Brassicae campestris dregs of rice, feather fan beanpod, the silo dust of cereal source mill, the processing waste of feed-processing plant and distillery exhausted grain products.
6. the method for claim 5, wherein said carbon source is the material through grinding or sieving.
7. claim 5 or 6 method, wherein said carbon source is the feather fan beanpod.
8. each method of aforementioned claim, wherein said microorganism mixes the group and comprises little algae, yeast, fungi, protobiont, microplankton and bacterium.
9. each method of claim 1-4, wherein said culturing step d) under saltwater environment, carry out.
10. the method for claim 9, wherein said culturing step d) in seawater, carry out.
11. each method of aforementioned claim, wherein said carbon-nitrogen ratio are about 2: 1 to about 24: 1 or are about 6: 1 to about 18: 1.
12. the method for claim 11, wherein said carbon-nitrogen ratio are 6: 1,12: 1 or 18: 1.
13. each method of aforementioned claim also comprises the step of optimizing gentle phosphorus-nitrogen ratio of nutrient water (P: N than) or silicon nitrogen ratio.
14. being selected from, each method of aforementioned claim, wherein said carbon source can be the material that described bacterium utilizes.
15. each method of aforementioned claim, wherein said culturing step d) carries out continuously decomposing and being converted into flocculant biological matter until most of carbon sources.
16. each method of aforementioned claim, the pH of wherein said culture systems remains under the pH of 7.3-8.3.
17. each method of aforementioned claim, microbe-derived in the wherein said step a) is the starter culture of naturally occurring one or more microorganisms in the culture systems water.
18. each method of aforementioned claim, the time that wherein said culturing step carries out is about 4-8 week.
19. claim 1,2 or 4 each methods, wherein said culturing step carries out in the length along container has the open container of constant depth.
20. the microbial biomass of producing by each method of claim 1-19.
A 21. feeds product or feed ingredient that comprises microbial biomass, comprise that also microorganism mixes the group, described microorganism mixes the group and comprises little algae and bacterium, and wherein said bacterium exists in the amount of the about 5-25wt% of dry-matter, and described little algae exists in the amount of dry-matter 10-80wt%.
22. the feeds product of claim 21 or feed ingredient, wherein said bacterium is present in the described microbial biomass in the amount of the about 20wt% of dry-matter 5-.
23. the feeds product of claim 21 or 22 or feed ingredient, wherein said bacterium is present in the described microbial biomass with the amount of the about 10wt% of about 5-of the microbial biomass of claim 20.
24. the microbial biomass of claim 20 or claim 21,22 or 23 feeds product are as the purposes of the aquaculture feed or the feed ingredient of water biological species.
25. the purposes of claim 24, wherein said water biological species is selected from fish, crustacean class or mollusc.
26. the purposes of claim 25, wherein said fish are selected from atlantic salmon, barramunda and cabio; Described crustacean class is selected from shrimp, lobster and crab; Described mollusc is selected from oyster, scallop and abalone.
27. a method of culturing water biological species comprises to the feed step of the microbial biomass of claim 20 of significant quantity or claim 21,22 or 23 feeds product of a kind of animal of water biological species.
28. the method for claim 27, wherein said water biological species is selected from fish, crustacean class or mollusc.
29. the method for claim 23, wherein said fish are selected from atlantic salmon, barramunda and cabio; Described crustacean class is selected from shrimp, lobster and crab; Described mollusc is selected from oyster, scallop and abalone.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2008201886 | 2008-04-30 | ||
| AU2008201886A AU2008201886B2 (en) | 2008-04-30 | 2008-04-30 | Microbial biomass, feed product/ingredient & processes for production thereof |
| PCT/AU2009/000539 WO2009132392A1 (en) | 2008-04-30 | 2009-04-30 | Microbial biomass, feed product/ingredient and processes for production thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610086266.1A Division CN105733981A (en) | 2008-04-30 | 2009-04-30 | Microbial biomass, feed product/ingredient and processes for production thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102016001A true CN102016001A (en) | 2011-04-13 |
Family
ID=41254701
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009801157074A Pending CN102016001A (en) | 2008-04-30 | 2009-04-30 | Microbial biomass, feed product/ingredient and processes for production thereof |
| CN201610086266.1A Pending CN105733981A (en) | 2008-04-30 | 2009-04-30 | Microbial biomass, feed product/ingredient and processes for production thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610086266.1A Pending CN105733981A (en) | 2008-04-30 | 2009-04-30 | Microbial biomass, feed product/ingredient and processes for production thereof |
Country Status (4)
| Country | Link |
|---|---|
| CN (2) | CN102016001A (en) |
| AU (2) | AU2008201886B2 (en) |
| BR (1) | BRPI0911787B1 (en) |
| WO (1) | WO2009132392A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911320A (en) * | 2014-03-12 | 2014-07-09 | 广东美瑞科海洋生物科技有限公司 | Biomass multi-culturing pond culturing method |
| CN104486953A (en) * | 2013-04-12 | 2015-04-01 | 联邦科学和工业研究组织 | Feed product and/or feed ingredient |
| CN106488707A (en) * | 2014-05-19 | 2017-03-08 | 汉斯-于尔根.德尔 | It is based primarily upon production method and the corresponding animal feed product for carnivore of the animal feed product of meat |
| CN112638168A (en) * | 2018-07-25 | 2021-04-09 | 联邦科学和工业研究组织 | Method for feeding fish |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102009019206B4 (en) * | 2009-04-28 | 2012-07-19 | Thyssenkrupp Polysius Ag | Process and plant for the utilization of gaseous and / or solid substances in exhaust gases |
| GB201017197D0 (en) * | 2010-10-12 | 2010-11-24 | Norwegian University Of Life Sciences The | Product |
| US9295206B2 (en) * | 2012-04-12 | 2016-03-29 | Johna Ltd | Method of culturing algae |
| MX354771B (en) * | 2012-04-12 | 2018-03-21 | Seagrass Ag/Sa/Ltd | Method of culturing algae. |
| GB2501101B (en) * | 2012-04-12 | 2014-06-11 | Seagrass Ag Sa Ltd | Method of culturing algae |
| CN103912790B (en) * | 2014-03-12 | 2016-08-17 | 广东美瑞科海洋生物科技有限公司 | The applied pipeline transportation method of the solid-state carbon source input that microbial biomass is cultivated and system |
| CN113016676B (en) * | 2019-12-25 | 2022-11-29 | 宁波大学 | Method for directionally culturing diatoms to culture litopenaeus vannamei |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002000035A1 (en) * | 2000-06-23 | 2002-01-03 | Acuabiotec Llc | Bioactive food complex, method for making bioactive food complex product and method for controlling disease |
| WO2004080196A2 (en) * | 2003-03-07 | 2004-09-23 | Advanced Bionutrition Corporation | Feed formulation for terrestrial and aquatic animals |
| CN1843102A (en) * | 2006-02-23 | 2006-10-11 | 庄文荣 | Shared deep water breeding method for braised abalones and sea slugs |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4958460A (en) * | 1988-05-09 | 1990-09-25 | Algae Farms | Method of growing and harvesting microorganisms |
| US20040009160A1 (en) * | 2001-06-22 | 2004-01-15 | Villamar Daniel F | Bioactive food complex, method for making bioactive food complex product and method for controlling disease |
| WO2004043139A2 (en) * | 2002-11-14 | 2004-05-27 | Advanced Bionutrition Corp. | Feed suitable for culturing rotifers, larval shrimp, and marine filter feeders |
| CN1735337A (en) * | 2002-11-26 | 2006-02-15 | 戈登·H·萨托 | Microbial feedstock for filter feeding aquatic organisms |
| US7776211B2 (en) * | 2006-09-18 | 2010-08-17 | Algaewheel, Inc. | System and method for biological wastewater treatment and for using the byproduct thereof |
-
2008
- 2008-04-30 AU AU2008201886A patent/AU2008201886B2/en active Active
-
2009
- 2009-04-30 WO PCT/AU2009/000539 patent/WO2009132392A1/en active Application Filing
- 2009-04-30 BR BRPI0911787-3A patent/BRPI0911787B1/en active IP Right Grant
- 2009-04-30 CN CN2009801157074A patent/CN102016001A/en active Pending
- 2009-04-30 CN CN201610086266.1A patent/CN105733981A/en active Pending
- 2009-10-13 AU AU2009225307A patent/AU2009225307B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002000035A1 (en) * | 2000-06-23 | 2002-01-03 | Acuabiotec Llc | Bioactive food complex, method for making bioactive food complex product and method for controlling disease |
| WO2004080196A2 (en) * | 2003-03-07 | 2004-09-23 | Advanced Bionutrition Corporation | Feed formulation for terrestrial and aquatic animals |
| CN1843102A (en) * | 2006-02-23 | 2006-10-11 | 庄文荣 | Shared deep water breeding method for braised abalones and sea slugs |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104486953A (en) * | 2013-04-12 | 2015-04-01 | 联邦科学和工业研究组织 | Feed product and/or feed ingredient |
| CN103911320A (en) * | 2014-03-12 | 2014-07-09 | 广东美瑞科海洋生物科技有限公司 | Biomass multi-culturing pond culturing method |
| CN103911320B (en) * | 2014-03-12 | 2016-10-05 | 广东美瑞科海洋生物科技有限公司 | Living beings many culture ponds cultural method |
| CN106488707A (en) * | 2014-05-19 | 2017-03-08 | 汉斯-于尔根.德尔 | It is based primarily upon production method and the corresponding animal feed product for carnivore of the animal feed product of meat |
| CN112638168A (en) * | 2018-07-25 | 2021-04-09 | 联邦科学和工业研究组织 | Method for feeding fish |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009132392A1 (en) | 2009-11-05 |
| AU2009225307B2 (en) | 2012-05-17 |
| BRPI0911787B1 (en) | 2019-06-04 |
| BRPI0911787A2 (en) | 2015-07-28 |
| BRPI0911787A8 (en) | 2018-02-06 |
| CN105733981A (en) | 2016-07-06 |
| AU2008201886B2 (en) | 2009-12-10 |
| AU2009225307A1 (en) | 2009-11-26 |
| AU2008201886A1 (en) | 2009-11-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2008201886B2 (en) | Microbial biomass, feed product/ingredient & processes for production thereof | |
| CN102018144B (en) | Japanese elver powdery formula feed | |
| CN101323843A (en) | Composite bacillus preparation for improving marine culture water environment and preparation thereof | |
| CN102018141B (en) | Anguilla japonica powdery mixed feed | |
| CN103283955A (en) | Method for preparing biological feed from rice straw | |
| Azim et al. | Intensifying aquaculture production through new approachesto manipulating natural food. | |
| CN102429127A (en) | Aquatic bio-feed | |
| CN105454105B (en) | A kind of rearing method of erythroculter ilishaeformis | |
| CN102100302A (en) | Preparation method of fermentation cake and maggot protein feed | |
| Coutteau et al. | Effect of algal ration and substitution of algae by manipulated yeast diets on the growth of juvenile Mercenaria mercenaria | |
| CN110868869A (en) | Zero-starch adhesive-free shrimp larva open-mouth compound feed and preparation method thereof | |
| CN103039727A (en) | Preparation method of stichopus japonicus fermented feed capable of replacing alga | |
| CN102150754A (en) | Organic Se-rich shrimp and breeding method thereof | |
| CN103086783B (en) | Fertilizer water paste and preparation method thereof | |
| CN1148939A (en) | Production of feedings for livestocks, poultry and aquatic livings by using industrial waste and EM bacteria | |
| CN105585377A (en) | Instant fish guano and preparation method thereof | |
| CN104486953A (en) | Feed product and/or feed ingredient | |
| CN102140041B (en) | Microbial fertilizer for shrimp pond and preparation method thereof | |
| CN103880544A (en) | Natural organic crop fertilizer and preparation method thereof | |
| CN100398001C (en) | Bait with enzymolysis being needed before use for shellfish | |
| KR101822736B1 (en) | Feed stuff for sea cucumber including organic compounds in biofloc and method for preparation thereof | |
| CN102326670A (en) | Selenium-enriched wheat fodder as well as preparation method and application thereof | |
| CN110800888A (en) | Composition for culturing plankton, preparation method and application thereof | |
| CN103848652A (en) | Preparation method of seaweed biological fertilizer | |
| CN114376113B (en) | Bait special for water spider and produced by raw materials prepared from Bacillus belgii and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1156661 Country of ref document: HK |
|
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110413 |
|
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1156661 Country of ref document: HK |