CN102388958A - Method for controlling grain pest by using essential oil - Google Patents
Method for controlling grain pest by using essential oil Download PDFInfo
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- CN102388958A CN102388958A CN2011103061732A CN201110306173A CN102388958A CN 102388958 A CN102388958 A CN 102388958A CN 2011103061732 A CN2011103061732 A CN 2011103061732A CN 201110306173 A CN201110306173 A CN 201110306173A CN 102388958 A CN102388958 A CN 102388958A
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- essential oil
- grain storage
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Abstract
The invention discloses a method for controlling grain pest by using essential oil. In the method, the essential oil and kieselguhr are mixed and used for controlling the grain pest. Compared with other methods for controlling the grain pest by using the essential oil, the method has the advantages that the kieselguhr is used as a carrier, and the volatilization of the essential oil can be delayed, so that the effects of the essential oil can be improved and efficiency duration can be prolonged; and by the method for controlling the grain pest by using the essential oil, the using amount of the essential oil can be greatly reduced and the pest killing effect of the essential oil is not influenced, and the pesticide using cost can be effectively reduced. The method has very wide application prospect in the agricultural technical fields of controlling the grain pest and the like.
Description
Technical field
The present invention relates to the grain storage pest controlling technical field, be specifically related to a kind of method of utilizing plants essential oil control grain storage pest.
Background technology
Grain is important goods and the strategic materials that involve the interests of the state and the people, and has crucial meaning for economic development and social stability.During foodstuff preservation, the loss that causes because of insect pest can not be ignored, according to relevant departments' investigation, and the loss of China's grain storage, national grain depot is 0.2%; The grain storage loss of peasant household is 6% ~ 9%.The annual production of China's grain at present surpasses 500,000,000 tons, and farmer storage accounts for more than 2/3.Therefore the raising of grain storage technology and very urgent to the control of grain storage pest.Though the grain storage technology of generally using at present can effectively be prevented and treated the harm of grain storage pest to a certain extent; But the drawback that they exist self also can not be ignored; Strict like mechanical-physical control for infrastructure etc.; Need a large amount of fund inputs, serious " 3R " problem that chemical prevention caused is then very serious.Therefore " green pesticide " of seeking a kind of environment-friendly type is extremely urgent.
Plants essential oil is one type of plant source secondary metabolites that different tissues position such as flower, leaf, fruit, the rhizome of plant etc. produces, and it derives from the Nature, draws materials extensively, and is free from environmental pollution, and noresidue is harmless to human health.Diatomite is a kind of silicastone, and main composition is noncrystal silica, also has the characteristics of low toxicity noresidue.Research at present shows that the control that plants essential oil or diatomite are applied to insect all has certain effect, but self also there is very important shortcoming in they, and is volatile like plants essential oil, so the insect protected term of validity is very short; And diatomite with increased the friction between the grain grain after grain mixes, reduced the unit weight and the flowability of grain, thereby reduced the grade of grain.
Through to the retrieval of the domestic and foreign literature of prior art, do not see that so far the relevant research that plants essential oil and diatomite mixture are applied to agricultural technology fields such as grain storage pest controlling reports.
Summary of the invention
The objective of the invention is to above-mentioned deficiency, a kind of method of utilizing plants essential oil control grain storage pest is provided to prior art.
The present invention realizes above-mentioned purpose through following technical scheme:
A kind of method of utilizing plants essential oil control grain storage pest is with plants essential oil and the control that is used further to grain storage pest after diatomite mixes.
With diatomite is carrier, utilizes diatomaceous strong adsorptivity to overcome the effumability of plants essential oil, plants essential oil and diatomite mixture is applied to the control of grain storage pest.The result finds under the condition that guarantees best insecticidal effect, only needs a spot of diatomite just can obviously reduce the consumption of plants essential oil and prolong it to act on the term of validity.
In the said method, said diatomite main component is noncrystal silica (quality percentage composition >=75%), and the method for this control grain storage pest is particularly effective for the control of red flour beetle.The preferred Chinese cassia tree of said plants essential oil (
Cinnamomum cassia) essential oil E8, allicin (
Allium sativum) essential oil E12 or the fruit of a cubeb litsea tree (
Litsea cubeba) essential oil E20.
The inventor confirms finally that through repeatedly screening above-mentioned essential oil can get at best prevention effect, the required cinnamon essential oil 400ml of grain promptly per ton, or allicin essential oil 350ml, or the sub-essential oil 450ml in the Cangshan when following consumption.
In the said method, diatomaceous consumption is also studied, found that grain per ton uses 250g diatomite; The essential oil that cooperates various dose again; Can reach preferable prevention effect, so the present invention prevents and treats in the method, the preferred grain consumption per ton of diatomite is 250g.
The method of preventing and treating of the present invention, preferred plant essential oil be with after diatomite mixes, and adopts to mix the stifling poisoning method of grain and carry out (being that mixture mixes the stirring placement with grain).
The method of preventing and treating of the present invention is preferably used in the seal storage place, avoids the unnecessary waste of essential oil because of excessively volatilizing and cause.
Compared with prior art, the present invention has following beneficial effect:
The method of plants essential oil control grain storage pest of utilizing of the present invention is compared with method with other plants essential oil, be carrier with diatomite, thereby the volatilization that delays plants essential oil improves its effect and lasting period; And used method through provided by the invention give, and can greatly reduce the consumption of plants essential oil and do not influence its insecticidal effect, can effectively reduce drug cost.The present invention is having boundless application prospect aspect the agricultural technology fields such as control of grain storage pest.
Description of drawings
Fig. 1. essential oil and with the diatomite mixture to the stifling toxic action duration analysis chart of red flour beetle adult, wherein E representes essential oil individual processing group, E+G representes essential oil and diatomite mixed processing group; E12 represents the allicin essential oil, and E8 represents cinnamon essential oil, and E20 represents the sub-essential oil in the Cangshan, and CK is a blank.
The specific embodiment
In following examples, used diatomite is that Guangdong grain science research institute provides, trade name match prestige.
With the red flour beetle is experimental subjects, adopts the stifling poisoning method of grain of mixing.
Get some parts of different amount plants essential oils: Chinese cassia tree (
Cinnamomum cassia) essential oil E8, allicin (
Allium sativum) essential oil E12 and the fruit of a cubeb litsea tree (
Litsea cubeba) essential oil E20 (every kind of essential oil consumption is respectively: 10 μ l, 15 μ l, 20 μ l, 25 μ l, 30 μ l and 40 μ l); Directly the essential oil with different amounts mixes with 50g paddy; Insert 60 of adults; With nylon gauze and preservative film all bottlenecks are sealed, put into 25 ℃ of temperature, observe waiting under the dark condition of humidity about 75%.
In essential oil and diatomite mixed processing group; Elder generation is with above-mentioned three kinds of essential oils (being respectively 0.5 μ l, 1.5 μ l, 2.0 μ l, 2.5 μ l, 3.0 μ l and 3.5 μ l) mixing of 12.5mg diatomite and different amounts; In this bottle, add paddy 50g, fully mixing again.Insert 60 of adults, with nylon gauze and preservative film all bottlenecks are sealed respectively, put into 25 ℃ of temperature, observe waiting under the dark condition of humidity about 75%.Two kinds of different contrasts are set: the blank CK that is left intact (only put into 50g paddy, insert 60 of adults) and only to add 12.5mg diatomite be control group G (12.5mg diatomite+50g paddy inserts 60 of adults).Every processing repetition five times.48h observes the death rate (touching the examination worm with the writing brush point, is death standard with the feeler nonresponder) after throwing worm, calculates different disposal group lethal concentration of 50 LC
50Reach parameters such as coefficient correlation.
The result finds the independent LC that handles with essential oil
50All greater than the LC of essential oil and diatomite mixture
50: three kinds of different essential oils of E12, E8 and E20 (separately) are to the LC of red flour beetle
50Be respectively 23.289 μ l/50g, 28.524 μ l/50g, 33.123 μ l/50g; And these three kinds of essential oils and diatomite mixture are to the LC of red flour beetle
50Then be merely 1.785 μ l/50g, 2.918 μ l/50g, 3.459 μ l/50g (seeing table 1).From LC
50Data can be known, are that the essential oil of carrier is given the insecticidal effect that can effectively improve plants essential oil with method with diatomite.
Table 1 plants essential oil reaches and the virulence of diatomite mixture to red flour beetle
Be with " * " person to represent that test of significance of coefficient of correlation is remarkable in 0.05 level in the table after the data; E12, E8 and E20 represent allicin essential oil, cinnamon essential oil and litsea cubeba oil respectively.
Embodiment 2 essential oils and with the mensuration of diatomite immixture duration
Essential oil and diatomite processing method are with embodiment 1 (the essential oil amount is that the examination worm death rate reaches about 90% institute's expenses in the 24h).The required essential oil concentration of processed group is E12 11 μ l (with 5mg diatomite mixture aequum 1.2 μ l), E8 11 μ l (with 5mg diatomite mixture aequum 1.2 μ l), E20 13 μ l (with 5mg diatomite mixture aequum 1.3 μ l).Handle the back and all bottlenecks are sealed, put into 25 ℃ of temperature with nylon gauze and preservative film, subsequent use under the dark condition of humidity about 75%.In the time of the 3rd day, 30 red flour beetles of hungry 24h are put into the organic paddy of the 20g that handles well, observe the death rate (touching the examination worm with the writing brush point, is death standard with the feeler nonresponder) of each group behind the 24h.Take out all examination worms in the processed group after the off-test of each time point; The bottleneck that different disposal paddy will be housed is again sealed in order to test use next time; Every red flour beetle that was reentered into hunger at a distance from three days is observed the death rate, test duration 30 days behind the 24h in each processed group bottle.Every processing repetition five times.
The result finds when handling 20 days; The examination worm death rate of three kinds of essential oil individual processing groups has all dropped to below 20%; And be the processed group of carrier administration with diatomite; Though its essential oil consumption is merely about 1/10 of individual processing group, the examination worm death rate of three processed group all maintains 40% left and right sides (see figure 1).Presentation of results is that the plants essential oil of carrier is given the lasting period that can effectively prolong plants essential oil with method with diatomite.
Embodiment 3: resistance realized heritability and the risk assessment of insect
When carrying out the estimation of genetics of resistance power, we consider resistance usually as a threshold character with normal distribution.The average of its distribution and variance are according to the dosage logarithm--and the regression equation of death rate probable value calculates.Concrete grammar with reference to Ding Wei etc. (fourth is big, Wang Jinjun, Zhao Zhimo, Zhang Yongqiang, Tao Huiying. l.bostrychophila air resistance and resistance to the action of a drug strain breeding and resistance risk analysis. plant protection journal, 2006,33 (1): 81-86).Simultaneously we also can estimate the genetic force situation of change of selecting many backs from generation to generation resistant population, thereby the time that pest resistance occurs is made prediction.According to the former study report, formula to calculating capable of using goes out the generation number that insect produces 10 times of required procreations of resistance.
We are through experiment and analyze discovery; Red flour beetle is less to the resistance realized heritability of allicin essential oil E12 and E12+G; Be respectively: 0.0550 (E12), 0.0225 (E12+G); Can calculate thus under the selection pressure of this test (average mortality is: essential oil individual processing group be 44.11% with essential oil and diatomite mixed processing group be 37.17%), make red flour beetle all produce 10 times resistance to essential oil E12 and with the diatomite mixture: essential oil individual processing group need screen for 384 generations altogether, was that carrier administration group need screen for 312 generations altogether with diatomite; This explanation is under these two kinds of processing modes, and the probability that insect develops immunity to drugs is all minimum.
This shows, be that the giving of plants essential oil of carrier use method with diatomite, not only can effectively reduce the consumption of plants essential oil, improves the insecticidal effect of plants essential oil, prolongs its effect lasting period, and also very little to the chance of this administering mode insect generation resistance.Therefore, the present invention is having boundless application prospect aspect the agricultural technology fields such as control of grain storage pest.
Claims (8)
1. method of utilizing plants essential oil control grain storage pest is characterized in that it being with plants essential oil and the control that is used further to grain storage pest after diatomite mixes.
2. according to the said method of utilizing plants essential oil control grain storage pest of claim 1, it is characterized in that said diatomite is that noncrystal dioxide-containing silica is more than 75%.
3. according to the said method of utilizing plants essential oil control grain storage pest of claim 1, it is characterized in that said grain storage pest is a red flour beetle.
4. according to the said method of utilizing plants essential oil control grain storage pest of claim 1, it is characterized in that said plants essential oil is cinnamon essential oil E8, allicin essential oil E12 and litsea cubeba oil E20.
5. according to the said method of utilizing plants essential oil control grain storage pest of claim 4, it is characterized in that the consumption of the required essential oil of grain per ton is following: cinnamon essential oil 400ml, allicin essential oil 350ml, the sub-essential oil 450ml in the Cangshan.
6. according to the said method of utilizing plants essential oil control grain storage pest of claim 1, it is characterized in that the required diatomite consumption of grain per ton is 250g.
7. according to the said method of utilizing plants essential oil control grain storage pest of claim 1, it is characterized in that it being to use in the seal storage place.
8. according to the said method of utilizing plants essential oil control grain storage pest of claim 1, it is characterized in that be with plants essential oil with after diatomite mixes, employing is mixed grain and is fumigated the poisoning method and carry out the control of grain storage pest.
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| CN103554347A (en) * | 2013-10-18 | 2014-02-05 | 江南大学 | Preparation method of oxide/ essential oil/ emulsion particle composite particle for after-finishing of article |
| CN104604860A (en) * | 2015-01-28 | 2015-05-13 | 中国农业科学院植物保护研究所 | Dimethyl disulfide granules and preparation method thereof |
| CN104621252A (en) * | 2015-02-11 | 2015-05-20 | 武汉轻工大学 | Allyl isothiocyanate and allicin compound and application thereof |
| CN104872280A (en) * | 2015-05-27 | 2015-09-02 | 闭炳春 | Cinnamon oil containing grain insecticide |
| CN104957191A (en) * | 2015-06-17 | 2015-10-07 | 湖州道场污水处理有限公司 | Plant essential oil insecticide efficient in poisoning rice weevils |
| CN105028512A (en) * | 2015-06-17 | 2015-11-11 | 湖州道场污水处理有限公司 | Preparation method for cinnamonandlavender-containing environment-friendly pesticide |
| CN105265574A (en) * | 2014-06-24 | 2016-01-27 | 中国农业大学 | Medicament capable of preventing and treating pests for stored grain, and application of medicament for preventing and treating pests for stored foods |
| CN106148005A (en) * | 2016-07-01 | 2016-11-23 | 西南大学 | A kind of embed food flavor microcapsule walking quickly and keeping away red flour beetle and preparation method thereof |
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| WO2017072563A1 (en) | 2015-10-29 | 2017-05-04 | Universidade Do Porto | Device for stored products protection and uses thereof |
| CN107136130A (en) * | 2017-05-09 | 2017-09-08 | 佛山文森特知识产权服务有限公司 | A kind of compound essential oil with antisepsis and sterilization effect and preparation method thereof |
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| CN108617656A (en) * | 2018-04-25 | 2018-10-09 | 华中农业大学 | The method that allicin interferes grain storage pest gelechiid reproductive behavio(u)r |
| CN109392899A (en) * | 2017-12-15 | 2019-03-01 | 广西壮族自治区农业科学院植物保护研究所 | A kind of plant source trypetid repellent Pesticidal combination |
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| CN108244107A (en) * | 2017-12-29 | 2018-07-06 | 邱清泉 | A kind of worm expelling bag for preventing medicinal material from damaging by worms and preparation method thereof |
| CN108617656A (en) * | 2018-04-25 | 2018-10-09 | 华中农业大学 | The method that allicin interferes grain storage pest gelechiid reproductive behavio(u)r |
| CN112931539A (en) * | 2021-03-12 | 2021-06-11 | 中南粮油食品科学研究院有限公司 | Rice composite insect-resist agent and preparation method and application thereof |
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| CN113016794A (en) * | 2021-03-29 | 2021-06-25 | 河北农业大学 | Preparation method of plant essential oil grain storage insecticidal slow release agent |
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