CN102370090A - Selenium-rich low-cholesterol duck egg and production method thereof - Google Patents
Selenium-rich low-cholesterol duck egg and production method thereof Download PDFInfo
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Abstract
The invention relates to a selenium-rich low-cholesterol duck egg and a production method thereof, belonging to the production field of healthcare functional eggs. In the invention, lactobacillus acidophilus and candida utilis are used as strains; the culture medium contains 0.5-2% of yeast extract paste and 0.5-2% of glucose; and the strains are inoculated in the culture medium containing sodium selenite to culture for 30-40 hours. The obtained selenium-rich probiotic culture solution is added to the corn bean pulp type basic ration to feed the laying duck; and one month later, the selenium-rich low-cholesterol duck eggs are obtained. While providing relatively comprehensive nutrition, the selenium-rich low-cholesterol duck egg produced by the invention plays the function of supplementing selenium, has the advantage of low cholesterol, has wide healthcare functions, and is particularly important in preventing cardiovascular diseases of human bodies.
Description
Technical field
The invention belongs to health care property egg product production technical field, particularly a kind of selenium-rich low-cholesterol duck's egg and production method thereof.
Technical background
Birds, beasts and eggs have very high nutritive value, and its trophic component is more comprehensive and balanced, and contain rich in protein, multiple unrighted acid, lecithin, cholesterol, vitamin and trace element etc., and be prone to digest and assimilate, be a kind of more satisfactory nutraceutical.Protein content is about 13%-15% in the birds, beasts and eggs; A little less than lean meat; But its amino acid whose composition and ratio thereof approach human body protein most; Wherein egg yolk protein is rich in human body beneficial's amino acid, like leucine, different amount amino acid, lysine, methionine, threonine, tryptophan, valine etc.Therefore the utilization rate of its protein is the highest, and shell egg is 94%, and yolk is 96%, in various food, ranks first.The fat content shell egg is 11%-15% in the birds, beasts and eggs, is 33% in the yolk, and fat is few in the egg white.Unrighted acid accounts for 60% in the fat, so fusing point is low, and easy quilt is digested and assimilated, and also contains the lecithin and the cholesterol of needed by human body.The cholesterol average content is 0.6%-0.7% in the shell egg, in the yolk up to 1.2%-1.8%.
Cholesterol is claimed cholesterine again, and a kind of derivative of cyclopentanoperhy drophenanthrene is the indispensable nutrient of health, is the main component of each cell membrane of human body, be present in the institute in a organized way in, the highest with content in the brain tissue.Cholesterol is not enough to influence growing of children, and the elderly's immunity kinetic energy reduces, and is prone to diseases such as cancer stricken, pneumonia.But; In recent years because the raising of people's living standard makes daily diet structure that very big variation take place, regular absorption animal meat, egg and edible wet goods; Higher meat and the egg of fat content ratio particularly; Will cause body inner cholesterol content to increase gradually, cause hypercholesterolemia, body is produced adverse influence.Modern study finds that atherosclerotic, phlebothrombosis formation and cholelithiasis and hypercholesterolemia have close correlation.The too high relation with angiocardiopathy of cholesterol has been a general knowledge that most of people know.Too much cholesterol in the blood can be deposited in blood vessel everywhere, has piled of a specified duration and will cause vascular sclerosis, obstruction, and caused angiocardiopathy or headstroke.Therefore foods such as egg and liver are kept someone at a respectful distance by many people.
Selenium is the human body trace elements necessary, is a kind of multi-functional life nutrient, has anti-oxidant; Regulate organism metabolism, improve immunity of organisms and premunition, improve and breed function; The opposing tumour; Prevent and treat endemic disease (like Keshan disease, Kaschin-Beck disease), delay senility multiple functions such as the poisonous element of antagonism.In recent years research shows that being related of the structure of selenium and cardiovascular system, function and disease thereof is close.Selenium enzyme-glutathione peroxidase has the reduction peroxide, anti peroxidation of lipid, the effect of protection cell membrane.Heart is very sensitive to lacking selenium, and selenium deficiency can cause MDA to be piled up, and the LDL oxidation damage impels the angiocardiopathy incidence and development.The incidence and development that lacks angiocardiopathies such as selenium and Keshan disease, atherosclerotic, coronary heart disease all has tangible correlation.
Selenium generally gets into human body through food chain, thereby human body selenium level and local Selenium in Soil level are closely related.Scarce selenium is worldwide problem, and China is in serious low selenium area, and the whole nation has 72% area to have scarce selenium to some extent.Therefore, mending selenium is the requisite measure of these regional population healths.It is reported; National at present selenium mean intake of being grown up is 26.6 microgram/days only; Almost only reach the necessary amounts lower limit 1/2 with 1/10 of better level; Therefore most people need increase the intake of selenium as early as possible, and should mend selenium throughout one's life, and mend the approach that selenium is relatively safe " multi-pronged " through the food of rich selenium.Regulation in " dietary guidelines of nutrient supply and China in the every day meals " that Chinese Soclety of Nutrition is recommended, the adult and teenager of essential intake of the day of selenium is 50 micrograms, children be 20 (1-3 year) to 40 (4-6 year) microgram, the baby is the 15-20 microgram; The highest dosis tolerata teenager (14-18 year) and grow up (more than 18 years old) are respectively 360 micrograms and 400 micrograms.
In sum; Birds, beasts and eggs have very high nutritive value; The incidence and development of angiocardiopathies such as the too much cholesterol and the shortage of selenium and atherosclerotic, coronary heart disease all has tangible correlation, if produce a kind of rich selenium and the lower duck's egg of cholesterol level, people can therefrom absorb abundant nutrition; Generation that again can angiocardiopathy preventing and send out morning helps lend some impetus to human healthy undoubtedly.
Summary of the invention
The object of the present invention is to provide all pure duck's eggs of the low courage of a kind of rich selenium and production method thereof.This egg product in the full nutrition, can be brought into play the function of mending selenium in that height is provided again, also has the advantage of low cholesterol, has health care widely, particularly playing an important role to angiocardiopathy preventing.
For realizing above-mentioned purpose, technical scheme of the present invention may further comprise the steps:
1) bacterial classification: lactobacillus acidophilus (L.acidophilus, LA) and candida utili (Candida utilis);
2) culture medium quality volume ratio is: 0.5%～2% yeast extract, 0.5%～2% glucose, pH nature; 100-120 ℃, high pressure steam sterilization 15min, subsequent use;
3) inorganic selenium source: the preparation selenium content is the sodium selenite solution of 0.5～5mg/ml, 100-125 ℃, and high pressure steam sterilization 30min, subsequent use;
4) activation of bacterial classification and seed liquor obtain:
The candida utili that is kept at 4 ℃ is inoculated on the slant medium, 20-30 ℃, cultivates 30-40h; After so continuous activation 3-5 time, be inoculated in the seed culture medium, 20-30 ℃, 220r/min, shaking table is cultivated 30-40h, obtains candida utili liquid seeds liquid;
The lactobacillus acidophilus that preserves is inoculated on the slant medium, 30-40 ℃, cultivates 30-40h; After so continuous activation 3-5 time, be inoculated in the seed culture medium, 30-40 ℃, cultivate 30-40h, obtain the lactobacillus acidophilus seed liquor;
5) fermentation:
Inorganic selenium source is added in the culture medium, make that selenium concentration is 4～10mg/kg in the culture medium; Candida utili seed liquor and lactobacillus acidophilus seed liquor inoculative proportion were inoculated in the culture medium in 1: 4 by volume, and total inoculum concentration is volume ratio 2%-5%; The Radix Astragali decocting liquid 0.5ml that when cultivating beginning, adds 1-5g oligosaccharides, 0.002-0.5g methionine, 0.002-0.5g serine, the 0.05-2.5g Radix Astragali/ml in every liter of culture medium; In cultivation temperature is 20-40 ℃, and throughput is 0.05m
3/ h, revolution are 70r/min, and tank pressure is to cultivate 30-40h under the condition of 0.04Mpa, cultivate and finish, and with the selenium-enriched probiotics nutrient solution branch that the obtains bucket of packing into, 4 ℃ of refrigerations, subsequent use;
6) production of selenium-rich low-cholesterol duck's egg:
The above-mentioned selenium-enriched probiotics nutrient solution that production method obtained is added in the corn dregs of beans type basal diet with the selenium level of 0.3～0.5mg/kg; The egg duck of feeding; Every day, feeding was 2 times; Free choice feeding and drinking-water, medication is undertaken by egg duck field conventional program with epidemic prevention, can obtain the selenium-rich low-cholesterol duck's egg after 1 month.
Beneficial effect:
The present invention utilizes organic selenium more to be prone to the principle that in duck's egg probios such as deposition, lactic acid bacteria can reduce the body inner cholesterol; Be bacterial classification at first with probio (mainly being lactic acid bacteria); Be inoculated in the culture medium that contains sodium selenite, go out to have the selenium-enriched probiotics nutrient solution of organic selenium and probio double action through fermenting and producing; Obtain the low cholesterol duck's egg of rich selenium then through the egg duck of feeding.
1, the duck's egg that obtains of the present invention is rich in selenium, nutritive value is complete and cholesterol level is lower.In than full nutrition, can bring into play the function of mending selenium providing high again, also have the advantage of low cholesterol, thereby have health care widely, particularly, be fit to the elderly and use the playing an important role of prevention people's angiocardiopathy.
2, the present invention obtain the selenium-rich low-cholesterol duck's egg more common duck's egg of Hough unit of at room temperature preserving the egg appearance of 5d significantly improve the holding time significant prolongation.
3, in the daily ration of egg duck, add the probiotics preparation that is rich in organic selenium and not only can obtain the low cholesterol duck's egg of rich selenium, and can significantly improve egg duck laying rate, day egg production, average egg weight, reduce feedstuff-egg ratio.
The specific embodiment
Below in conjunction with embodiment the present invention is done further description:
(1), is rich in the production of probiotic's culture (fermentation) liquid of organic selenium
1) bacterial classification: lactobacillus acidophilus (L.acidophilus, LA): preserve the center available from the Institute of Microorganism, Academia Sinica microorganism fungus kind, the bacterial strain code name is AS1.1878; Candida utili (Candida utiliis): press and smooth available from Chinese Academy of Sciences microorganism and to study carefully the institute microorganism fungus kind and preserve the center, the bacterial strain code name is AS2.1180; Be the feed grade probio bacterial classification that the Ministry of Agriculture allows use.
2) culture medium: the 1kg yeast extract, 1kg glucose is dissolved in 100L water, the pH nature; 115 ℃, high pressure steam sterilization 15min, subsequent use.
3) inorganic selenium source: preparation Se concentration is the sodium selenite solution 750ml of 1mg/ml, 120 ℃, and high pressure steam sterilization 30min, subsequent use.
4) activation of bacterial classification and seed liquor obtain:
The candida utili that is kept at 4 ℃ is inoculated on the slant medium, 28 ℃, cultivates 32h; After the so continuous activation 3 times, be inoculated in the 0.5L seed culture medium, 28 ℃, 220r/min, shaking table is cultivated 32h, obtains candida utili liquid seeds liquid;
The lactobacillus acidophilus that preserves is inoculated on the slant medium, 37 ℃, cultivates 32h; After the so continuous activation 3 times, be inoculated in the 2L seed culture medium, 37 ℃, cultivate 32h, obtain the lactobacillus acidophilus seed liquor.
5) fermentation: 750ml sodium selenite (1mg/ml is in Se) solution is added in the 97L culture medium; Candida utili seed liquor and lactobacillus acidophilus seed liquor inoculative proportion were inoculated in the culture medium by 1: 4, and total inoculum concentration is 2.5L; Add organic selenium inversion accelerating agent: add sterilized 200g oligosaccharides, 1g methionine, 1g serine, the Radix Astragali decocting liquid (50ml of the 0.2g Radix Astragali/ml) in the culture medium; Cultivation temperature: 30 ℃; Throughput: 0.05m
3/ h; Revolution: 70r/min; Tank pressure: 0.04Mpa; Fermentation time: 36h; After the fermentation ends, with the selenium-enriched probiotics nutrient solution branch bucket of packing into, 4 ℃ of refrigerations, subsequent use.
The cfu number of candida utili and lactobacillus acidophilus is respectively 10 in the probiotic's culture that is rich in organic selenium (fermentation) liquid of above-mentioned acquisition
9/ mL and 10
11/ mL; Selenium content is 7.5mg/L, and wherein organic selenium content is more than 90%, and selenomethionine content is more than 75%.
(2) probiotic's culture (fermentation) liquid that is rich in organic selenium is fed the egg duck:
1) implements animal and grouping
Experiment selected 400 plumages 26 week egg duck in age (laying rate is about 90%) is divided into 2 groups at random, every group of 5 repetitions, and each repeats 40 plumages.1 group is that control group is raised with corn dregs of beans type basis egg duck daily ration (table 1); 2 groups are added 0.5mg.kg in its basal diet
-1The probio of being rich in organic selenium of selenium, every day, feeding was 2 times, free choice feeding and drinking-water, medication is undertaken by egg duck field conventional program with epidemic prevention.
2) sample collection and mensuration
Test duration 3 months is organized to appoint at random each repetition and get 2 pieces of eggs (each handles 10 pieces of eggs) every month from each, and 4 ℃ of refrigerators are preserved duck's egg selenium to be measured and cholesterol level, detect and are undertaken by conventional method.
3) result of implementation
Visible by table 1, to compare with control group, interpolation contains 0.5mgkg in the feed
-1The duck's egg Se content of the selenium-enriched probiotics of selenium test back 1 month, 2 months and 3 months raise respectively 167.40% (P＜0.01), 163.35% (P＜0.01) and 174.47% (P＜0.01).
Table 1 egg duck daily ration add selenium-enriched probiotics to the influence of duck's egg Se content (mg/kg, weight in wet base, n=10)
Visible by table 2, to compare with control group, interpolation contains 0.5mgkg in the feed
-1The selenium-enriched probiotics of selenium has reduced by 18.69% (P＜0.01), 2Q.84% (P＜0.01) and 20.88% (P＜0.01) respectively in the yolk cholesterol concentration of test back 1 month, 2 months and 3 months.
Table 2 egg duck daily ration add selenium-enriched probiotics to the influence of duck's egg cholesterol concentration (mg/g, n=10)
Through present embodiment, visible test group duck's egg Se content is 0.484-0.516mg/kg (weight in wet base), and cholesterol level of vitellus is 11.29-12.57mg/g (weight in wet base); And basal diet group duck's egg Se content is merely 0.177-0.189mg/kg (weight in wet base), and cholesterol level of vitellus reaches 14.23-14.96mg/g (weight in wet base).
Claims (1)
1. selenium-rich low-cholesterol duck's egg and production method thereof is characterized in that: may further comprise the steps:
1) bacterial classification: lactobacillus acidophilus (L.acidophilus, LA) and candida utili (Candida utilis);
2) culture medium quality volume ratio is: 0.5%～2% yeast extract, 0.5%～2% glucose, pH nature; 100-120 ℃, high pressure steam sterilization 15min, subsequent use;
3) inorganic selenium source: the preparation selenium content is the sodium selenite solution of 0.5～5mg/ml, 100-125 ℃, and high pressure steam sterilization 30min, subsequent use;
4) activation of bacterial classification and seed liquor obtain:
The candida utili that is kept at 4 ℃ is inoculated on the slant medium, 20-30 ℃, cultivates 30-40h; After so continuous activation 3-5 time, be inoculated in the seed culture medium, 20-30 ℃, 220r/min, shaking table is cultivated 30-40h, obtains candida utili liquid seeds liquid;
The lactobacillus acidophilus that preserves is inoculated on the slant medium, 30-40 ℃, cultivates 30-40h; After so continuous activation 3-5 time, be inoculated in the seed culture medium, 30-40 ℃, cultivate 30-40h, obtain the lactobacillus acidophilus seed liquor;
5) fermentation:
Inorganic selenium source is added in the culture medium, make that selenium concentration is 4～10mg/kg in the culture medium; Candida utili seed liquor and lactobacillus acidophilus seed liquor inoculative proportion were inoculated in the culture medium in 1: 4 by volume, and total inoculum concentration is volume ratio 2%-5%; The Radix Astragali decocting liquid 0.5ml that when cultivating beginning, adds 1-5g oligosaccharides, 0.002-0.5g methionine, 0.002-0.5g serine, the 0.05-2.5g Radix Astragali/ml in every liter of culture medium; In cultivation temperature is 20-40 ℃, and throughput is 0.05m
3/ h, revolution are 70r/min, and tank pressure is to cultivate 30-40h under the condition of 0.04Mpa, cultivate and finish, and with the selenium-enriched probiotics nutrient solution branch that the obtains bucket of packing into, 4 ℃ of refrigerations, subsequent use;
6) production of selenium-rich low-cholesterol duck's egg:
The above-mentioned selenium-enriched probiotics nutrient solution that production method obtained is added in the corn dregs of beans type basal diet with the selenium level of 0.3～0.5mg/kg; The egg duck of feeding; Every day, feeding was 2 times; Free choice feeding and drinking-water, medication is undertaken by egg duck field conventional program with epidemic prevention, can obtain the selenium-rich low-cholesterol duck's egg after 1 month.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816707A (en) * | 2012-07-24 | 2012-12-12 | 福建倪氏微生物科技有限公司 | Candida utilis strain and application of same in preparation of selenium-rich yeast products |
| CN103549221A (en) * | 2013-11-22 | 2014-02-05 | 安徽科技学院 | Feed additive for ducks producing selenium-rich rhizoma curcumae longae duck eggs |
| CN104489249A (en) * | 2014-05-09 | 2015-04-08 | 重庆达军食品有限责任公司 | Low-cholesterol ecological egg production method |
| CN105494259A (en) * | 2015-12-28 | 2016-04-20 | 安徽富硒香生物食品股份有限公司 | Method for producing duck eggs rich in organic selenium and zinc |
| CN106417918A (en) * | 2015-07-29 | 2017-02-22 | 钟泰源 | Livestock and poultry additive containing selenium and EM probiotics and application of additive |
| CN107699505A (en) * | 2017-10-19 | 2018-02-16 | 四川农业大学 | A kind of optimization method and technique of Se-enriched yeast culture process |
| CN111887101A (en) * | 2020-07-22 | 2020-11-06 | 湖北民族大学 | Preparation method and application of selenium-rich pleurotus citrinopileatus mycelium |
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2011
- 2011-09-13 CN CN 201110269821 patent/CN102370090A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816707A (en) * | 2012-07-24 | 2012-12-12 | 福建倪氏微生物科技有限公司 | Candida utilis strain and application of same in preparation of selenium-rich yeast products |
| CN103549221A (en) * | 2013-11-22 | 2014-02-05 | 安徽科技学院 | Feed additive for ducks producing selenium-rich rhizoma curcumae longae duck eggs |
| CN103549221B (en) * | 2013-11-22 | 2015-05-06 | 安徽科技学院 | Feed additive for ducks producing selenium-rich rhizoma curcumae longae duck eggs |
| CN104489249A (en) * | 2014-05-09 | 2015-04-08 | 重庆达军食品有限责任公司 | Low-cholesterol ecological egg production method |
| CN106417918A (en) * | 2015-07-29 | 2017-02-22 | 钟泰源 | Livestock and poultry additive containing selenium and EM probiotics and application of additive |
| CN105494259A (en) * | 2015-12-28 | 2016-04-20 | 安徽富硒香生物食品股份有限公司 | Method for producing duck eggs rich in organic selenium and zinc |
| CN105494259B (en) * | 2015-12-28 | 2018-10-12 | 安徽富硒香生物食品股份有限公司 | A kind of production method of richness Organic Selenium zinc duck's egg |
| CN107699505A (en) * | 2017-10-19 | 2018-02-16 | 四川农业大学 | A kind of optimization method and technique of Se-enriched yeast culture process |
| CN107699505B (en) * | 2017-10-19 | 2020-12-04 | 四川农业大学 | Optimization method and process of selenium-enriched yeast culture process |
| CN111887101A (en) * | 2020-07-22 | 2020-11-06 | 湖北民族大学 | Preparation method and application of selenium-rich pleurotus citrinopileatus mycelium |
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Application publication date: 20120314 |