CN107125736A - A kind of preparation method of raspberry ferment - Google Patents
A kind of preparation method of raspberry ferment Download PDFInfo
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- CN107125736A CN107125736A CN201710309532.7A CN201710309532A CN107125736A CN 107125736 A CN107125736 A CN 107125736A CN 201710309532 A CN201710309532 A CN 201710309532A CN 107125736 A CN107125736 A CN 107125736A
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention discloses a kind of preparation method of raspberry ferment, including screening, color protection, pulverize and sieve, fermented pretreatment, plant bacterium, again through filtering, it is qualified to examine, it is filling, produce raspberry ferment step, the present invention make use of the nutritive value of raspberry well, the potential value of raspberry is expanded, it is all good that a kind of mouthfeel is provided for people, with high nutritive value raspberry ferment product, with mitigation enteron aisle load, activate healthy cycle system, adjust microecological balance, strengthen human body cell vigor, purify blood, enhance metabolism, inspire human body cell vigor, strengthen metabolic function, lift the effect of body rejuvenation, heart disease, hepatopathy chronic diseases have certain prevention effect, there is soothing the liver be amusing, the effect of enriching blood, long-term consumption also can soft skin, play a part of beauty face-whitening-nourishing, it can be widely applied to health products or cosmetic and cosmetics.
Description
Technical field
The invention belongs to ferment preparing technical field, more particularly to a kind of preparation method of raspberry ferment.
Background technology
Raspberry, Chinese medicine name.Also known as：Cover basin, black Fischer, small pallet, raspberry.Early summer fruit by it is green become it is greenish-yellow when harvest.
Medicinal part：Dry fruit；Nature and flavor：Sweet, acid, it is warm-natured；Channel tropism：Return liver, kidney, bladder warp；Effect：Supplementing the kidney to control the nocturnal contracting urine, is supported
Liver improving eyesight；Cure mainly：For emission and spermatorrhea, enuresis frequent micturition, impotence and premature ejaculation, mesh is secretly dim-sighted.Usage and dosage：6~12g.With kidney-nourishing
Astringent sperm contracting urine, the effect of nourishing the liver to improve visual acuity.It is usually used in emission and spermatorrhea, enuresis frequent micturition, impotence and premature ejaculation, mesh is secretly dim-sighted.《Explaining abstruseness of the canon of materia medica》：
" raspberry, it is sweet to put down into kidney, play a sun and control impotence, controlling nocturnal emission with astringent drugs take the photograph it is excessive, strong kidney and without it is scorching it is partially, controlling nocturnal emission with astringent drugs and undoubtedly puckery evil, the product of gold and jade
." raspberry can the effectively angiocardiopathy such as allevating angina pectoris, but cause slight diarrhoea sometimes.Fruit is sour-sweet, there is " yellow
The good reputation of Jinsui River fruit ", containing materials such as abundant salicylic acid, phenolic acid, long-term consumption can effectively cardioprotection, prevent high blood
The cardiovascular and cerebrovascular diseases such as pressure, vascular wall atherosis, cardiovascular and cerebrovascular embrittlement.
Ferment is also known as " enzyme ", and ferment is a kind of protein smaller than cell-unit, is that a kind of living things catalysis that carries is made
Specific proteins.In its underwriter's body in metabolism various Biochemical changes mediator, being metabolized of all cells, it is newborn,
Decompose, digest etc., it all can't do without it.There is ferment just to have life；There is no ferment then to mean that life will be walked to death.Enzyme exists
Do not acted on directly in ferment product, the enzymatic efficiency of microorganism is interior with the unique and efficient of fermentoid without human body first
Property；Enzyme in second human body has specific structures locating, tissue positioning and Subcellular Localization, and these positioning are genetic determinations
, external enzyme can not be positioned specifically；3rd, external enzyme is easily digested system hydrolysis, is also easily recognized and degrades by immune system；
4th, the stability of enzyme is poor, long-time and high temperature, high pressure, the easy in inactivation such as peracid parlkaline.
Ferment refers to using pure plants such as one or more plants, vegetables and fruits, seed, herbal medicine, marine algas as raw material, through multiple beneficial
What bacterium was fermented and produced, the micro- life of the feature containing nutritional ingredients such as abundant vitamin, enzyme, mineral matter and secondary metabolites
Thing fermented product.Ferment product is that biological raw material is formed through microbial fermentation or bioconversion, with certain biological function
Biological agent.But, the raw material of selection is differed, specific preparation method different, its main component because of producer's difference
It is variant.The specific effect of ferment is relevant with formula, is all to stimulate cellular gene expression, production by the physiologically active ingredient of formation
Some raw enzymes, inhibition of gene expression reduces some enzymatic activitys, so as to adjust metabolism.
In fact, doing ferment product has four main purposes：(1) in order to more effectively utilize food materials, due to environmental pollution, agriculture
Medicine residual, antibiotic, operating pressure, the beneficial flora of human body have been subjected to larger destruction, it is difficult to which effectively conversion food materials synthesize generation
Thank to Auto-regulator, and external probiotics fermention provides such a platform；(2) be conducive to material to extract, be dissolved in water；(3) have
Beneficial to the removing conversion of harmful substance, toxicity is reduced；(4) be conducive to fresh-keeping, delay the inactivation of active ingredient.
The process that ferment makes, is exactly put into gastral transformation system in vitro, the probiotics system made good use of more has
The conversion of the completion material of effect.The making of ferment mainly has three kinds of methods：(1) classical facture, raw material add brown sugar and water system
Make, Chinese Shanxi generation is come across earliest, the fermented tea in 453 years Christian eras is exactly earliest.Happy element is female to be done with reference to this.(2)
The facture of fruit and vegetable etc. raw material sugaring, the method released by Japanese with reference to fermented tea, current Taiwan, Japan are more
In this way.(3) raw material sugaring and probiotics fermention facture, this is that ferment makes new method.Especially plus honey and
The method of plant source lactobacillus ferment, comparatively facilitates the synthesis and conversion of growth of probiotics and functional mass.
The characteristics of new method ferment makes：(1) it is formulated, according to the succession of edible medicinal formula since ancient times, modern times in ancient times food medicine
With material composition and functional characteristics, targetedly ferment main material formula is devised functionally, in fermentation process,
Conversion and synthesis for functional mass provide favourable basis；(2) probiotics, according to main material raw material using being conducive to functional mass
The probiotics system extracted, convert and synthesized, catalyst is provided for the formation of functional mass；(3) technique, according to modern times fermentation
Technology, selected be adapted to growth of probiotics and material conversion fermentation system and zymotechnique, make functional mass formation and
Fermentation condition and fermentation time that the removing of harmful substance is optimal.
Fermentation imparts the major class function of ferment four：(1) function of being determined by composition of raw materials, this partial function in itself is general not
Easily converted by probiotics；(2) by raw material through probiotics convert formed by new material and the function that determines, without specific former
Material, specific probiotics are difficult to form such material；(3) function that the material synthesized by probiotics is determined, such
Functional mass is only relevant with probiotics；(4) function that the eucaryotic cell structure material of probiotics in itself is determined.Fruit juice only has the first work(
Can, and probiotics preparation only has latter two function.So, ferment can allow what the maximally effective utilization main material raw material of consumer was provided
Nutritional ingredient, the gene expression of the corresponding enzyme of human activin cell improves metabolic activity.
The function of ferment depends primarily on the following aspects：(1) the main material formula of science, is not that species is The more the better,
Species is few, and single composition does not reach demand threshold, and be equal to nothing function, and many risk increases mutually gram of species, produces poison
The risk increase of element；(2) suitable strain, harmful bacteria easily grows if strain is not connect, it is impossible to provide the function of the bacterium determined,
Connecing probiotics can be conducive to material to be converted into the thing beneficial to human body, improve body or environment flora, assign predetermined function；
(3) fermentation technique of science, is conducive to functional mass to synthesize, harmful substance conversion, reduces harmful bacteria and grows risk, microorganism life
Long rapid, metabolism is vigorous, and material conversion can be just completed in a short time；(4) raw material of high-quality.
The activity of ferment is different because of the age, and by the baby that is born to infancy, the activity of ferment is increase and continuous
Promote；Into after the middle age, the problems such as due to life style and environmental pollution, the activity decrease of ferment, metabolic function are also because of year
Age constantly fails.Ferment needed for original human body can be absorbed completely from daily fruit-vegetable plant food to but ferment
Element is very fragile, and it will lose activity completely more than 60 degree.Why the Chinese for liking heating to cook, seriously lack
Weary ferment is mainly this reason.
The content of the invention
It is an object of the invention to：For above-mentioned problem, there is provided a kind of preparation method of raspberry ferment.This hair
Bright raspberry ferment proteinase activity power is higher, nutritive value enrich, take more convenient, exocellular polysaccharide, lactic acid bacteria etc. it is beneficial into
Divide and dramatically increase；It is also added into grape juice：Cantaloupe juice：Honeysuckle powder, adds the mouthfeel of raspberry ferment product so that shape
Into product taste be easily esthetically acceptable to the consumers.The present invention make use of the nutritive value of raspberry well, expand raspberry
Potential value, provide that a kind of mouthfeel is all good, with high nutritive value raspberry ferment product for people, and to heart disease,
Hepatopathy chronic diseases have certain prevention effect, have the effect of it is soothing the liver be amusing, enrich blood, long-term consumption also can soft skin, play
The effect of beauty face-whitening-nourishing.
In order to achieve the above object, present invention employs following technical scheme：
A kind of preparation method of raspberry ferment, comprises the following steps：
(1) screen：Selection is fresh, big, ripe raspberry, by screening, removes raspberry stalk, leaf and other are different
Thing, cleans and is drained on placement screen cloth；
(2) color protection：Raspberry is poured into color retention is carried out in color protection solution, taken out, placed on screen cloth after 3~5h of immersion
Drain；
(3) pulverize and sieve：According to weight ratio it is 1 by the raspberry of step (2) and drinking water：It is even that high speed is carried out after 1 mixing
Slurry processing, is crossed unsifted raspberry and drinking water after 100~200 mesh with 2：High-speed homogenization processing is carried out again after 1 mixing, is obtained
To uniform raspberry paste；
(4) fermented pretreatment：The pectase of enzymolysis processing and enzyme-deactivating processing, pectase are added in being pasted to raspberry
Addition is 1500U/g, and pectase polyoses content is 2.5%, through sterilization treatment after being well mixed, and obtains sterile raspberry enzymolysis
Liquid；
(5) bacterium is planted for the first time：The saccharomyces cerevisiae of its weight 3~5% is added to the sterile raspberry enzymolysis liquid of step (4),
Ferment 6~10d under the conditions of being placed in 30~35 DEG C, stirs daily twice, and 100~200 mesh sieves excessively, which are produced, after fermentation ends once filters
Liquid；
(6) second of plant bacterium：Added into the first-time filtrate of step (5) haw pulp of its weight 7~10%, Calusena lansium sauce,
Grape juice, Cantaloupe juice, honeysuckle powder, are well mixed, and the acetic acid of liquid sauce mixture weight 1.2~2% is added under stirring
Bacteria culture fluid, 12~16d of fermentation obtains zymotic fluid；
(7) third time plants bacterium：Lactic acid bacteria is accessed to the zymotic fluid of step (6), is placed in 32~36 DEG C of fermenting house and carries out
Fermentation, obtains enzyme liquid；
(8) above-mentioned enzyme liquid is qualified, filling through filtering, examining again, produces raspberry ferment.
Further, in step (2), the color protection solution is by citric acid and water according to weight ratio 3~7：100 mixing systems
.
Further, in step (4), the time of the enzymolysis processing is 2.5h, and hydrolysis temperature is 45 DEG C, and pH value is
4.5。
Further, in step (5), the saccharomyces cerevisiae first cultivates 1.5~3d under the conditions of 20~35 DEG C.
Further, in step (6), the haw pulp：Calusena lansium sauce：Grape juice：Cantaloupe juice：Honeysuckle powder：Matrimony vine
Powder：Longan powder：Red date powder=1：1：1：1：1：1：1：1；The acetic acid bacteria culture fluid is that acetic acid bacteria cultivates 2~4d in nutrient solution
Gained, cultivation temperature is 20~35 DEG C, and viable count is 108~109Individual/ml.
Further, in step (7), the lactobacillus inoculum amount is 4.5~5.5%；The enzyme liquid pH=5.5~
6.5, proteinase activity force value is 945U/g.
Further, in step (7), the fermentation condition is：6~8d of lucifuge fermentation.
The raspberry that the present invention reuses sweet temperature is primary raw material, with liver-kidney tonifying, astringent sperm contracting urine, improving eyesight effect, contain
Have a materials such as abundant salicylic acid, phenolic acid, long-term consumption can effectively cardioprotection, preventing hypertension, vascular wall atherosis,
The cardiovascular and cerebrovascular diseases such as cardiovascular and cerebrovascular embrittlement；With haw pulp, Calusena lansium sauce, grape juice, Cantaloupe juice, honeysuckle powder, wolfberry fruit powder, dragon
Eye powder, red date powder are as auxiliary material, and the raspberry ferment obtained using the preparation method of the present invention contains protein, carbon hydrate
Thing, a variety of natural complex, mineral matter, amino acid and trace element etc..
Raspberry ferment can be rapidly entered in human body cell, improve cell function, and repairs cell injured in human body, plus
Strong general metabolic function, is adjusted to general body state；Fat in blood is decomposed, helps human body to remove in each organ-tissue and gives up
Thing, improves each organ dysfunction, to a variety of chronic diseases of human body, such as：Hypertension, high fat of blood, diabetes, rheumatism etc. have the improvement state of an illness
Effect；There is purification blood, decompose septicemia element, improve blood viscosity, help human body anti-oxidant, remove free radical, improve
Immune function of human body, dispelling fatigue, and can help human body skin except spot, disappear acne.Improve skin elasticity；Ferment has to human body cell
Activation, excludes dead cell, promotes the division of healthy cell regeneration, coordinates whole body endocrine, reaches health
One new height.It is particularly suitable for use in (1) valetudinarian；(2) it is busy with one's work, the big person of pressure；(3) sleep insufficiency person；(4) stomach
Function is not good enough, and complexion is partially black or dark sore spot person；(5) blood viscosity is high, blood fat height or the high person of blood glucose；(6) cerebral thrombus, headstroke person
Deng.Nutritionist points out that people should also supplement some ferment while replenishing vitamins are with replenishing the calcium, although it is not battalion
Element is supported, but contains various enzymes in ferment, nutrient is needed with the help of it, could be decomposed, absorb and profit by body
With.So, supplement ferment is the new ideas of human nutrition, is the new method of adjuvant treatment of diseases, can build up resistance, play
The effect of health care.
Experimental facilities of the present invention：Pulverizer；Mixer；Temperature controller；The hole thermostat water bath of biserial four：Changzhou is state-run
Testing equipment research institute HH-S4；Autoclave sterilization machine：Hangzhou section dawn chemical industry experimental instruments and equipment limited YXQ-LS；Sterile behaviour
Make platform：Suzhou hundred million reaches cleaning laboratory equipment Co., Ltd；ELIASA：Bai Teng Instrument Ltd. of the U.S. (BioTek)
Cytation3。
The assay method of raspberry polysaccharide of the present invention：Using phend-sulphuric acid, the percentage composition of polysaccharide=pass through standard is bent
The glucose content that line is measured/(polysaccharide concentration × be used to survey the polysaccharide volume that absorbance is taken) × %；Proteinase activity power is surveyed
Determine method：The enzyme activity force value of the protease in zymotic fluid is determined using national standard SB/T10317-1999.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
(1) present invention selects the probiotic strain beneficial to human body intestinal canal, strictly controls fermentation temperature, effectively reduces and cover basin
The loss of active ingredient in son, remains raspberry enzyme activity to greatest extent；Using three implantation dominant bacterias, effectively suppress
The growth of other miscellaneous bacterias, it is ensured that the stabilization of product quality, and acidophilus, thermophilic, Lactobacillus plantarum are selected, these bacterial strains are demonstrate,proved
Real to have symbiosis performance, mixed fermentation can be colonized in enteron aisle, while being conducive to being lifted the synthetic activity of ferment product.It is prepared into
To raspberry ferment have certain prevention effect to heart disease, hepatopathy chronic diseases, have the effect of it is soothing the liver be amusing, enrich blood, it is long
Phase it is edible also can soft skin, play a part of beauty face-whitening-nourishing.
(2) preparation condition of the present invention simulates the natural formation condition of ferment, and scientific in principle, equipment is simply suitable to extensive raw
Production, obtained raspberry ferment is never the health food having no toxic side effect containing artificial color, chemical spices and preservative, tool
There is pure natural feature.The main component of ferment also has trace element, mineral matter, a variety of natural dimension lifes in addition to multiple eating ferment
Element, amino acid, protein, carbohydrate etc.；With enteron aisle load is mitigated, healthy cycle system is activated, regulation Tiny ecosystem is put down
Weighing apparatus, strengthens human body cell vigor, purifies blood, enhances metabolism, and inspires human body cell vigor, strengthens metabolic function,
Lift the effect of body rejuvenation.It can be widely applied to health products or cosmetic and cosmetics.
(3) raspberry ferment proteinase activity power of the present invention is higher, and nutritive value enriches, and takes more convenient, exocellular polysaccharide,
The beneficiating ingredients such as lactic acid bacteria are dramatically increased；It is also added into grape juice：Cantaloupe juice：Honeysuckle powder, adds the production of raspberry ferment
The mouthfeel of product so that the product taste of formation is easily esthetically acceptable to the consumers.The present invention make use of the nutriture value of raspberry well
Value, has expanded the potential value of raspberry, provides that a kind of mouthfeel is all good, with high nutritive value raspberry ferment produces for people
Product.
Embodiment
For the objects, technical solutions and advantages of the present invention are more clearly understood, preferred embodiment is enumerated below, to this hair
It is bright to be further described.However, it is necessary to which many details listed in explanation, specification are used for the purpose of making reader to this
The one or more aspects of invention have a thorough explanation, can also realize the present invention's even without these specific details
These aspects.
Embodiment 1
A kind of preparation method of raspberry ferment, comprises the following steps：
(1) screen：Selection is fresh, big, ripe raspberry, by screening, removes raspberry stalk, leaf and other are different
Thing, cleans and is drained on placement screen cloth；
(2) color protection：Raspberry is poured into color retention is carried out in color protection solution, taken out after immersion 5h, placed and dripped on screen cloth
It is dry；The color protection solution is by citric acid and water according to weight ratio 7：100 are mixed to prepare；
(3) pulverize and sieve：According to weight ratio it is 1 by the raspberry of step (2) and drinking water：It is even that high speed is carried out after 1 mixing
Slurry processing, is crossed unsifted raspberry and drinking water after 200 mesh with 2：High-speed homogenization processing is carried out again after 1 mixing, is obtained
Even raspberry paste；
(4) fermented pretreatment：The pectase of enzymolysis processing and enzyme-deactivating processing, pectase are added in being pasted to raspberry
Addition is 1500U/g, and pectase polyoses content is 2.5%, through sterilization treatment after being well mixed, and obtains sterile raspberry enzymolysis
Liquid；The time of the enzymolysis processing is 2.5h, and hydrolysis temperature is 45 DEG C, and pH value is 4.5；
(5) bacterium is planted for the first time：The saccharomyces cerevisiae of its weight 5% is added to the sterile raspberry enzymolysis liquid of step (4), is placed in
Ferment 10d under the conditions of 35 DEG C, and stirring daily crosses 200 mesh sieves twice, after fermentation ends and produces first-time filtrate；The saccharomyces cerevisiae is first
3d is cultivated under the conditions of 35 DEG C；
(6) second of plant bacterium：The haw pulp, Calusena lansium sauce, grape of its weight 10% are added into the first-time filtrate of step (5)
Juice, Cantaloupe juice, honeysuckle powder, are well mixed, and the acetic acid bacteria culture fluid of liquid sauce mixture weight 2% is added under stirring,
Fermentation 16d obtains zymotic fluid；The haw pulp：Calusena lansium sauce：Grape juice：Cantaloupe juice：Honeysuckle powder：Wolfberry fruit powder：Longan powder：It is red
Date powder=1：1：1：1：1：1：1：1；The acetic acid bacteria culture fluid is that acetic acid bacteria is cultivated in nutrient solution obtained by 4d, and cultivation temperature is
35 DEG C, viable count is 108~109Individual/ml；
(7) third time plants bacterium：Access and fermented in lactic acid bacteria, the fermenting house for being placed in 36 DEG C to the zymotic fluid of step (6),
Obtain enzyme liquid；The lactobacillus inoculum amount is 5.5%；Enzyme liquid pH=5.5~6.5, proteinase activity force value is
945U/g；The fermentation condition is：Lucifuge fermentation 8d；
(8) above-mentioned enzyme liquid is qualified, filling through filtering, examining again, produces raspberry ferment.
Embodiment 2
A kind of preparation method of raspberry ferment, comprises the following steps：
(1) screen：Selection is fresh, big, ripe raspberry, by screening, removes raspberry stalk, leaf and other are different
Thing, cleans and is drained on placement screen cloth；
(2) color protection：Raspberry is poured into color retention is carried out in color protection solution, taken out after immersion 3h, placed and dripped on screen cloth
It is dry；The color protection solution is by citric acid and water according to weight ratio 3：100 are mixed to prepare；
(3) pulverize and sieve：According to weight ratio it is 1 by the raspberry of step (2) and drinking water：It is even that high speed is carried out after 1 mixing
Slurry processing, is crossed unsifted raspberry and drinking water after 100 mesh with 2：High-speed homogenization processing is carried out again after 1 mixing, is obtained
Even raspberry paste；
(4) fermented pretreatment：The pectase of enzymolysis processing and enzyme-deactivating processing, pectase are added in being pasted to raspberry
Addition is 1500U/g, and pectase polyoses content is 2.5%, through sterilization treatment after being well mixed, and obtains sterile raspberry enzymolysis
Liquid；The time of the enzymolysis processing is 2.5h, and hydrolysis temperature is 45 DEG C, and pH value is 4.5；
(5) bacterium is planted for the first time：The saccharomyces cerevisiae of its weight 3% is added to the sterile raspberry enzymolysis liquid of step (4), is placed in
Ferment 6d under the conditions of 30 DEG C, and stirring daily crosses 100 mesh sieves twice, after fermentation ends and produces first-time filtrate；The saccharomyces cerevisiae is first
1.5d is cultivated under the conditions of 20 DEG C；
(6) second of plant bacterium：The haw pulp, Calusena lansium sauce, grape of its weight 7% are added into the first-time filtrate of step (5)
Juice, Cantaloupe juice, honeysuckle powder, are well mixed, and the acetic acid bacteria culture of liquid sauce mixture weight 1.2% is added under stirring
Liquid, fermentation 12d obtains zymotic fluid；The haw pulp：Calusena lansium sauce：Grape juice：Cantaloupe juice：Honeysuckle powder：Wolfberry fruit powder：Longan
Powder：Red date powder=1：1：1：1：1：1：1：1；The acetic acid bacteria culture fluid is that acetic acid bacteria is cultivated in nutrient solution obtained by 2d, is cultivated
Temperature is 20 DEG C, and viable count is 108~109Individual/ml；
(7) third time plants bacterium：Access and fermented in lactic acid bacteria, the fermenting house for being placed in 32 DEG C to the zymotic fluid of step (6),
Obtain enzyme liquid；The lactobacillus inoculum amount is 4.5%；Enzyme liquid pH=5.5~6.5, proteinase activity force value is
945U/g；The fermentation condition is：Lucifuge fermentation 6d；
(8) above-mentioned enzyme liquid is qualified, filling through filtering, examining again, produces raspberry ferment.
Embodiment 3
A kind of preparation method of raspberry ferment, comprises the following steps：
(1) screen：Selection is fresh, big, ripe raspberry, by screening, removes raspberry stalk, leaf and other are different
Thing, cleans and is drained on placement screen cloth；
(2) color protection：Raspberry is poured into color retention is carried out in color protection solution, taken out after immersion 4h, placed and dripped on screen cloth
It is dry；The color protection solution is by citric acid and water according to weight ratio 5：100 are mixed to prepare；
(3) pulverize and sieve：According to weight ratio it is 1 by the raspberry of step (2) and drinking water：It is even that high speed is carried out after 1 mixing
Slurry processing, is crossed unsifted raspberry and drinking water after 150 mesh with 2：High-speed homogenization processing is carried out again after 1 mixing, is obtained
Even raspberry paste；
(4) fermented pretreatment：The pectase of enzymolysis processing and enzyme-deactivating processing, pectase are added in being pasted to raspberry
Addition is 1500U/g, and pectase polyoses content is 2.5%, through sterilization treatment after being well mixed, and obtains sterile raspberry enzymolysis
Liquid；The time of the enzymolysis processing is 2.5h, and hydrolysis temperature is 45 DEG C, and pH value is 4.5；
(5) bacterium is planted for the first time：The saccharomyces cerevisiae of its weight 4% is added to the sterile raspberry enzymolysis liquid of step (4), is placed in
Ferment 8d under the conditions of 32 DEG C, and stirring daily crosses 150 mesh sieves twice, after fermentation ends and produces first-time filtrate；The saccharomyces cerevisiae is first
2.3d is cultivated under the conditions of 29 DEG C；
(6) second of plant bacterium：The haw pulp, Calusena lansium sauce, grape of its weight 8% are added into the first-time filtrate of step (5)
Juice, Cantaloupe juice, honeysuckle powder, are well mixed, and the acetic acid bacteria culture of liquid sauce mixture weight 1.6% is added under stirring
Liquid, fermentation 14d obtains zymotic fluid；The haw pulp：Calusena lansium sauce：Grape juice：Cantaloupe juice：Honeysuckle powder：Wolfberry fruit powder：Longan
Powder：Red date powder=1：1：1：1：1：1：1：1；The acetic acid bacteria culture fluid is that acetic acid bacteria is cultivated in nutrient solution obtained by 3d, is cultivated
Temperature is 28 DEG C, and viable count is 108~109Individual/ml；
(7) third time plants bacterium：Access and fermented in lactic acid bacteria, the fermenting house for being placed in 34 DEG C to the zymotic fluid of step (6),
Obtain enzyme liquid；The lactobacillus inoculum amount is 5.0%；Enzyme liquid pH=5.5~6.5, proteinase activity force value is
945U/g；The fermentation condition is：Lucifuge fermentation 7d；
(8) above-mentioned enzyme liquid is qualified, filling through filtering, examining again, produces raspberry ferment.
In summary, the present invention selects the probiotic strain beneficial to human body intestinal canal, strictly controls fermentation temperature, effectively reduction
The loss of active ingredient, remains raspberry enzyme activity to greatest extent in raspberry；Using three implantation dominant bacterias, have
Effect inhibits the growth of other miscellaneous bacterias, it is ensured that the stabilization of product quality, and selects acidophilus, thermophilic, Lactobacillus plantarum, these bacterial strains
Symbiosis performance is had been shown to have, mixed fermentation can be colonized in enteron aisle, while being conducive to being lifted the synthetic activity of ferment product.
Preparation condition of the present invention simulates the natural formation condition of ferment, and scientific in principle, equipment is simply suitable to large-scale production, and what is obtained covers
Basin ferment is never the health food having no toxic side effect, with pure natural spy containing artificial color, chemical spices and preservative
Point.The main component of ferment in addition to multiple eating ferment, also have trace element, mineral matter, a variety of natural complex, amino acid,
Protein, carbohydrate etc..Raspberry ferment proteinase activity power of the present invention is higher, and nutritive value enriches, and takes more convenient,
The beneficiating ingredients such as exocellular polysaccharide, lactic acid bacteria are dramatically increased；It is also added into grape juice：Cantaloupe juice：Honeysuckle powder, adds and covers basin
The mouthfeel of sub- ferment product so that the product taste of formation is easily esthetically acceptable to the consumers.The present invention make use of raspberry well
Nutritive value, expanded the potential value of raspberry, provided that a kind of mouthfeel is all good, with high nutritive value covers basin for people
Sub- ferment product, with enteron aisle load is mitigated, activates healthy cycle system, adjusts microecological balance, strengthen human body cell vigor,
Blood is purified, is enhanced metabolism, human body cell vigor is inspired, strengthens metabolic function, the effect of body rejuvenation is lifted,
Heart disease, hepatopathy chronic diseases have certain prevention effect, have the effect of it is soothing the liver be amusing, enrich blood, long-term consumption also can soft skin
Skin, plays a part of beauty face-whitening-nourishing, can be widely applied to health products or cosmetic and cosmetics.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of preparation method of raspberry ferment, it is characterised in that comprise the following steps：
(1) screen：Selection is fresh, big, ripe raspberry, by screening, removes raspberry stalk, leaf and other foreign matters, washes
Net place drains on screen cloth；
(2) color protection：Raspberry is poured into color retention is carried out in color protection solution, taken out after 3~5h of immersion, placed and dripped on screen cloth
It is dry；
(3) pulverize and sieve：According to weight ratio it is 1 by the raspberry of step (2) and drinking water：Carried out after 1 mixing at high-speed homogenization
Reason, is crossed unsifted raspberry and drinking water after 100~200 mesh with 2：High-speed homogenization processing is carried out again after 1 mixing, is obtained
Even raspberry paste；
(4) fermented pretreatment：The pectase of enzymolysis processing and enzyme-deactivating processing is added in being pasted to raspberry, pectase is added
Measure as 1500U/g, pectase polyoses content is 2.5%, through sterilization treatment after being well mixed, obtain sterile raspberry enzymolysis liquid；
(5) bacterium is planted for the first time：The saccharomyces cerevisiae of its weight 3~5% is added to the sterile raspberry enzymolysis liquid of step (4), is placed in
Ferment 6~10d under the conditions of 30~35 DEG C, and stirring daily crosses 100~200 mesh sieves twice, after fermentation ends and produces first-time filtrate；
(6) second of plant bacterium：The haw pulp, Calusena lansium sauce, grape of its weight 7~10% are added into the first-time filtrate of step (5)
Juice, Cantaloupe juice, honeysuckle powder, are well mixed, and the acetic acid bacteria training of liquid sauce mixture weight 1.2~2% is added under stirring
Nutrient solution, 12~16d of fermentation obtains zymotic fluid；
(7) third time plants bacterium：Access and fermented in lactic acid bacteria, the fermenting house for being placed in 32~36 DEG C to the zymotic fluid of step (6),
Obtain enzyme liquid；
(8) above-mentioned enzyme liquid is qualified, filling through filtering, examining again, produces raspberry ferment.
2. a kind of preparation method of raspberry ferment according to claim 1, it is characterised in that：It is described in step (2)
Color protection solution is by citric acid and water according to weight ratio 3~7：100 are mixed to prepare.
3. a kind of preparation method of raspberry ferment according to claim 2, it is characterised in that：It is described in step (4)
The time of enzymolysis processing is 2.5h, and hydrolysis temperature is 45 DEG C, and pH value is 4.5.
4. a kind of preparation method of raspberry ferment according to claim 1, it is characterised in that：It is described in step (5)
Saccharomyces cerevisiae first cultivates 1.5~3d under the conditions of 20~35 DEG C.
5. a kind of preparation method of raspberry ferment according to claim 4, it is characterised in that：It is described in step (6)
Haw pulp：Calusena lansium sauce：Grape juice：Cantaloupe juice：Honeysuckle powder：Wolfberry fruit powder：Longan powder：Red date powder=1：1：1：1：1：1：1：1；
The acetic acid bacteria culture fluid is that acetic acid bacteria is cultivated in nutrient solution obtained by 2~4d, and cultivation temperature is 20~35 DEG C, and viable count is
108~109Individual/ml.
6. a kind of preparation method of raspberry ferment according to claim 5, it is characterised in that：It is described in step (7)
Lactobacillus inoculum amount is 4.5~5.5%；Enzyme liquid pH=5.5~6.5, proteinase activity force value is 945U/g.
7. a kind of preparation method of raspberry ferment according to claim 5, it is characterised in that：It is described in step (7)
Fermentation condition is：6~8d of lucifuge fermentation.
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Application publication date: 20170905 |