CN108013305A - A kind of preparation method of sprouted unpolished rice ferment drink - Google Patents
A kind of preparation method of sprouted unpolished rice ferment drink Download PDFInfo
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- CN108013305A CN108013305A CN201610970723.3A CN201610970723A CN108013305A CN 108013305 A CN108013305 A CN 108013305A CN 201610970723 A CN201610970723 A CN 201610970723A CN 108013305 A CN108013305 A CN 108013305A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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Abstract
The present invention relates to a kind of preparation method of sprouted unpolished rice ferment drink, use aspergillus oryzae, the mode of Rhizopus oryzae mixed culture handles sprouted unpolished rice, sprouted unpolished rice is decomposed using its enzyme system is complementary, and inoculating lactic acid bacterium ferments, obtained sprouted unpolished rice ferment drink is rich in organic acid, various free amino acids, soluble dietary fiber and active ingredient gamma aminobutyric acid, SOD etc., sweet mouthfeel, drink conveniently, without additive and there is good nutritional health function, fermenting twice process greatly improves raw material availability, current brown rice is largely solved because mouthfeel is difficult to the wasting of resources that is brought receiving.
Description
Technical field
The present invention relates to fermentation engineering field, more specifically, be related to one kind carries out fermentation system using sprouted unpolished rice
Sprouted unpolished rice ferment drink obtained and preparation method thereof.
Background technology
Brown rice is that paddy sloughs the caryopsis after outer protection cortex rice husk, and interior protection cortex (pericarp, kind skin, megarchidium layer) is intact
Rice Kernel.Traditional rice eliminates chaff layer and plumule, only remains endosperm fraction during polished rice is processed into,
And about 64% nutrient is accumulated in chaff layer and plumule in brown rice, isolating protein, fat, carbohydrate, mineral matter, dimension
Outside the main nutrient compositions such as raw element and cellulose, also rich in the nutrient for participating in human body physiological effect and regulatory function, such as sub- oil
Acid, VE, oryzanol, γ-aminobutyric acid, squalene, n-octacosanol, rice bran-fiber, choline, phosphatide, inositol, glutathione,
Peroxidase, selenium, magnesium and manganese etc..
Sprouted unpolished rice has become a research hotspot at present.Research shows that brown rice is in germination process, its internal generation
Acute variation, produces or is enriched with the various components for having health care and function of bodybuilding.Research is found：Resist in sprouted unpolished rice containing abundant
Active oxygen phytic acid, forulic acid etc., can suppress the generation of melanin, skin is kept fair and clear, and can boost metabolism, and prevent
Artery sclerosis, viscera function obstacle and cancer etc..Substantial amounts of amino acid GABA is present in brain and spinal cord, and having, which improves blood, follows
Ring, increase oxygen supply amount, suppress vegetative dystonie and senile dementia and other effects.In addition, sprouted unpolished rice also maintains
Trace element necessary to the human bodies such as more magnesium, potassium, calcium, zinc and iron.
However, since brown rice peel layer is rich in fiber, quality is close, therefore coarse mouthfeel, it is difficult to swallows, is nothing like pure white
Rice is welcome like that.China produces about 200,000,000 tons of paddy per year, but traditional pure white rice processing method is caused up to 9,600,000~19,200,000 tons
The wasting of resources of high nutrient is at present, also very big with western developed country gap in this aspect China.As a whole, state
Inside realize that the brown rice product quantity of industrialization is relatively fewer, product is single.Either can sprouted unpolished rice that directly boiling is eaten also
It is the sprouted unpolished rice food of fast food and the snack food using sprouted unpolished rice as dispensing, brown rice resource full of nutrition cannot obtains
To making full use of.
Chinese CN 101946943A patents of invention disclose a kind of system of germinated brown rice milk beverage by compound lactobacillus fermentation
Preparation Method, prepares Lactobacillus rhamnosus, Pediococcus acidilactici, streptococcus thermophilus, four strains of lactic acid bacteria direct putting type of Lactobacillus brevis hair first
Ferment agent；Then by brown rice at 30 DEG C, when immersion 10 is small, when 29 DEG C of germinations 12 are small, sprouted unpolished rice is prepared；Sprouted unpolished rice is added
3 times of water use colloid abrasive slurry, after being gelatinized, being saccharified, by liquid rice milk per ton add 150 grams of throw type leaven, 38 DEG C-
40 DEG C, it is static fermentation 6 it is small when；Remove the gred by 5000 revs/min of centrifugations, by the allotment of supernatant volume addition xylitol, doughnut
143 DEG C are used after membrane filtration exquisiteness, 2 seconds, ultra high temperature short time sterilization, filling was pillow type packaging or Lile packaging, is finished product.
This method enriches the species of probiotics, and the nutrition of product is lifted simultaneously also by brown rice germination.However, raw material is pasted through high temperature
Change, nutritive loss is larger after saccharification.
Using the brown rice to germinate as primary raw material, it is prepared Chinese CN 101077092A patents of invention germinated brown rice yoghourt
Method mainly includes immersion, mashing, liquefaction, saccharification, allotment, homogeneous, sterilization, fermentation, stirring, canned and refrigeration.Its advantage is
Starch Conversion is the sugar of low molecular weight by two enzymes method, and lactic fermentation is carried out after being mixed with skimmed milk power, can not only be incited somebody to action
Wherein active ingredient is dissolved into juice, and can also eliminate peculiar smell and deposited phenomenon.But in order to keep the steady of brown rice yoghourt
Qualitative to need to add substantial amounts of additive, the acceptance of consumer is relatively low.
The content of the invention
The technical problem to be solved in the present invention is：A kind of preparation method of sprouted unpolished rice ferment drink is provided, is solved existing
There is the problems such as sprouted unpolished rice fermented product raw material availability is low, nutritive loss is larger, needs to add substantial amounts of additive.
The present invention to solve the above-mentioned problems, there is provided technical solution be：A kind of system of sprouted unpolished rice ferment drink is provided
Preparation Method, includes the following steps：
S100, brown rice germination：It is raw material to select high-quality brown rice, drains germination after being soaked in warm water, and sprouted unpolished rice is steamed
Ripe postcooling；
S200, mold fermentation：The aspergillus oryzae prepared, Rhizopus oryzae are inoculated into the sprouted unpolished rice of boiling, 28~37 DEG C
Constant temperature incubation obtains the first zymotic fluid in 1~4 day；
S300, lactobacillus-fermented：The water of 3~5 times of quality is added into first zymotic fluid, addition mass fraction is 2
~6% sugar, inoculation quality fraction is 4~6% lactic acid bacteria after sterilization, and 12~24h is left to ferment under the conditions of 30~37 DEG C；Cross
Filter, gained supernatant liquid obtain sprouted unpolished rice ferment drink after sterilization.
In the preparation method for the sprouted unpolished rice ferment drink that the present invention improves, the step S100 specifically includes following step
Suddenly：
S101, the high-quality brown rice of selection are raw material, after cleaning, disinfection, 6~12h are soaked in 28~35 DEG C of thermostatted waters, after draining
Germinate, it is 30~35 DEG C to keep germination environment temperature, grows 1~2.5mm to bud and terminates to germinate；
S102, according to the ratio that the mass ratio of sprouted unpolished rice and water is 1: 1~1: 2 add water, heating steam it is ripe to rice, after by its
Postcooling is broken up to room temperature.
In the preparation method for the sprouted unpolished rice ferment drink that the present invention improves, in the step S101, in brown rice raw material
Including at least one colored rice brown rice.
In the preparation method for the sprouted unpolished rice ferment drink that the present invention improves, the step S200 specifically includes following step
Suddenly：
S201, prepare aspergillus oryzae solid medium, Rhizopus oryzae fluid nutrient medium respectively, and sterilizing cooling is stand-by；
S202, by the aspergillus oryzae of preservation, Rhizopus oryzae actication of culture, be seeded to aspergillus oryzae solid medium, Rhizopus oryzae respectively
Fluid nutrient medium；30 DEG C of quiescent culture 72h of aspergillus oryzae, the during which loose culture medium of regular shaking flask；34 DEG C of Rhizopus oryzae, 200rpm shaking tables
Cultivate 60h；
S203, will add in aspergillus oryzae solid medium and hanged with the isometric distilled water of solid medium, obtained aspergillus oryzae
Liquid；Into the sprouted unpolished rice of boiling be inoculated with aspergillus oryzae, Rhizopus oryzae, total inoculum concentration for 1~1.5%, 28~37 DEG C of constant temperature incubations 1~
4 days, the volume ratio of aspergillus oryzae suspension and Rhizopus oryzae nutrient solution was 1: 1~2: 1.
In the preparation method for the sprouted unpolished rice ferment drink that the present invention improves, the step S300 specifically includes following step
Suddenly：
S301, fermentation medium prepare：The water of 3~5 times of quality is added into first zymotic fluid, adds mass fraction
For 2~6% sugar, 100 DEG C, 8~12h of room temperature after 30min sterilizations, carry out again 100 DEG C, the sterilization of 30min hair is made
Ferment culture medium；
It is S302, inoculum fermented：In the fermentation medium inoculation quality fraction be 4~6% lactic acid bacteria, 30~37 DEG C
Under the conditions of be left to ferment 12~24h；
S303, filtering, sterilization：Supernatant liquid will be obtained after fermentation gained filtering fermentation liquor, sprouted unpolished rice ferment is obtained after sterilization
Plain drink.
In the preparation method for the sprouted unpolished rice ferment drink that the present invention improves, in the step S301, the sugar is white
At least one of granulated sugar, fructose, glucose, rock sugar
In the preparation method for the sprouted unpolished rice ferment drink that the present invention improves, in the step S302, the lactic acid bacteria
For lactobacillus plantarum and Lactobacillus casei Mixed Microbes, wherein, the mass ratio of lactobacillus plantarum and Lactobacillus casei is 3: 1~10:
1。
In the preparation method for the sprouted unpolished rice ferment drink that the present invention improves, in the step S303, the zymotic fluid
Supernatant liquid is obtained after diatomite filters, pH3~4, sterilization conditions are 135 DEG C, 3~4s.
The present invention selects brown rice to produce full nutrition sprouted unpolished rice ferment for raw material, prebiotic Pseudomonas food-grade microorganisms step fermentation
Plain drink, compares and has the advantage that with related brown rice product at present on the market and existing processing technology：
(1) product sweet mouthfeel of the present invention, it is convenient, it is additive-free, it is good for one's health.
(2) producing enzyme of product utilization of the present invention aspergillus oryzae and Rhizopus oryzae is complementary, is replaced with microbial process traditional
Two enzymes method, fully decomposes sprouted unpolished rice, and not only high degree reduces the loss of nutrition, and the metabolic activity of microorganism make it is proper
Further a large amount of dissolution antiactive oxygen phytic acid, forulic acid, trace element in system, improve the value of product.
(3) further producing or improve by germination treatment early period and the fermentation of later stage multiple-microorganism various has
Health care and the component of function of bodybuilding.After testing, this product is rich in organic acid, various free amino acids, soluble dietary fiber and work
Property component γ-aminobutyric acid, SOD etc..Particularly there is the alpha-aminobutyric acid content alleviated heart and brain blood pressure, adjust immune function
It is significantly improved, there is more preferable nutritional health function.
(4) it is high to meet industrial requirement, no security risk, and raw material availability for product of the present invention.
Brief description of the drawings
Below in conjunction with accompanying drawings and embodiments, the invention will be further described, in attached drawing：
Fig. 1 is the flow chart of the preparation method preferred embodiment of sprouted unpolished rice ferment drink of the present invention.
Embodiment
The embodiment of the present invention is specifically described below in conjunction with attached drawing.
The preparation method of fermentative brown rice drink is, it is necessary to using two enzymes method so that starch saccharification is so as to lactic acid bacteria in the prior art
Utilize, such a mode is very low for the utilization rate of other compositions in brown rice, causes a large amount of foodstuff wastes；And some are in the prior art
Brown rice germination can be handled, the type and quantity of bioactive substance in drink can be greatly improved, but be gelatinized through high temperature, sugar
Loss of the process of change for bioactive substance is larger so that corresponding health-care efficacy is given a discount.In addition, directly use two enzymes method
The brown rice of processing is not germinated unpolished rice nutritious comprehensive, is not to be especially advantageous for lactic acid bacteria production.
The main innovation point of the present invention is that the mode being mixed using aspergillus oryzae, Rhizopus oryzae handles sprouted unpolished rice, profit
The resolution ratio of sprouted unpolished rice, small molecule carbohydrate, micromolecule polypeptide, the free amino acid of generation are greatly improved with its enzyme system complementarity
And the trace element of dissolution is not only present directly in drink and is beneficial to absorption of human body, but also the growth of lactic acid bacteria can be promoted,
Raw material availability is further improved, while the bioactive substance that the mode of biological enzymolysis will not also bring brown rice germination to generate loses
It is living, there is more preferable nutritional health function.
Fig. 1 is the flow chart of the preparation method preferred embodiment of sprouted unpolished rice ferment drink of the present invention, as shown in Figure 1, this
The preparation method of embodiment sprouted unpolished rice ferment drink, includes the following steps：
S100, brown rice germination：It is raw material to select high-quality brown rice, drains germination after being soaked in warm water, and sprouted unpolished rice is steamed
Ripe postcooling；Germinating process of brown rice similarly to the prior art, adds water to cook and not only kills miscellaneous bacteria so that follow-up mold fermentation has
Strain advantage, can also make its structure more dispersed, beneficial to the growth of follow-up aspergillus oryzae, Rhizopus oryzae.
S200, mold fermentation：The aspergillus oryzae prepared, Rhizopus oryzae are inoculated into the sprouted unpolished rice of boiling, 28~37 DEG C
Constant temperature incubation obtains the first zymotic fluid in 1~4 day；Aspergillus oryzae, being used in mixed way for Rhizopus oryzae can be fully decomposed in sprouted unpolished rice
Albumen and starch, and cause other nutrient releases such as soluble dietary fiber, trace element, brown rice germination will not also be produced
Raw γ-aminobutyric acid small molecule bioactive material causes damages.
S300, lactobacillus-fermented：The water of 3~5 times of quality is added into first zymotic fluid, addition mass fraction is 2
~6% sugar, inoculation quality fraction is 4~6% lactic acid bacteria after sterilization, and 12~24h is left to ferment under the conditions of 30~37 DEG C；Cross
Filter, gained supernatant liquid obtain sprouted unpolished rice ferment drink after sterilization.Due to the decomposition of aspergillus oryzae, Rhizopus oryzae for sprouted unpolished rice
Effect is more preferable, and nutritional ingredient is more balanced, the lactobacillus-fermented carried out than two enzymes method processing sprouted unpolished rice, lactic acid bacteria of the present invention
Cell concentration can improve 15%~25%.
Preferably, the brown rice raw material used in the present invention includes at least one colored rice brown rice.Such as green rice, red rice,
Black rice, these colored rices are than the content higher of the nutrients such as the albumen of general brown rice, trace element, the sprouted unpolished rice after decomposition
Not only be more favorable for the growth of lactic acid bacteria, and after germinateing γ-aminobutyric acid small molecule bioactive material content higher.
Preferably, the lactic acid bacteria is lactobacillus plantarum and Lactobacillus casei Mixed Microbes, wherein, lactobacillus plantarum and cheese
The mass ratio of lactobacillus is 3: 1~10: 1.The mixing of lactobacillus plantarum and Lactobacillus casei, can make up single kind lactic acid bacteria
Nutrition Preference, more fully using raw material while so that drink mouthfeel is more abundant, soft.What the present invention used
Lactobacillus plantarum is purchased from Wuhan Xinhuayang Biological Co., Ltd with species L. casei.
Preferably, aspergillus oryzae, the preparation method of Rhizopus oryzae are as follows in the present invention, after obtained aspergillus oryzae, Rhizopus oryzae are used for
Continue in each embodiment：
(1) aspergillus oryzae solid medium, Rhizopus oryzae fluid nutrient medium are prepared respectively, and sterilizing cooling is stand-by；Wherein：
Aspergillus oryzae solid medium：20 grams of wheat bran, 15 grams of bean cake powder, 10 grams of flour, water 40ml.
Rhizopus oryzae fluid nutrient medium (g/L)：Glucose 140g/L, urea 2.0g/L, KH2PO40.3g/L, NaH2PO4
0.08g/L, MgSO4·7H20 0.25g/L, ZnSO4·7H20 0.08g/L.Disposably add the CaCO individually to sterilize3 50g/
L。
(2) aspergillus oryzae of preservation, Rhizopus oryzae strain are activated using PDA culture medium, is seeded to the training of aspergillus oryzae solid respectively
Support base, Rhizopus oryzae fluid nutrient medium；30 DEG C of quiescent culture 72h of aspergillus oryzae, the during which loose culture medium of regular shaking flask；Rhizopus oryzae 34
DEG C, 200rpm shaking table cultures 60h.It is limited that aspergillus oryzae that the present invention uses, Rhizopus oryzae strain purchased from Wuhan Xinhua raise biological share
Company.
Embodiment 1
The preparation method of the sprouted unpolished rice ferment drink of the present embodiment, includes the following steps：
(1) brown rice germination：It is raw material to select high-quality brown rice, after cleaning, disinfection, 12h is soaked in 28 DEG C of thermostatted waters, after draining
Germinate, it is 30 DEG C to keep germination environment temperature, terminates to germinate to the long 1mm of bud；
(2) cook：Add water according to the ratio of mass ratio 1: 1 according to sprouted unpolished rice and water, heating steam it is ripe to rice, after by its dozen
Postcooling is dissipated to room temperature；
(3) mold fermentation：The aspergillus oryzae prepared, Rhizopus oryzae are inoculated into boiling sprouted unpolished rice after cooling, 28 DEG C
Constant temperature incubation obtains the first zymotic fluid in 4 days；Will in aspergillus oryzae solid medium add with the isometric distilled water of solid medium,
Aspergillus oryzae suspension is made；Into the sprouted unpolished rice of boiling be inoculated with aspergillus oryzae, Rhizopus oryzae, total inoculum concentration be 1%, aspergillus oryzae suspension and
The volume ratio of Rhizopus oryzae nutrient solution is 1: 1；
(4) lactobacillus-fermented：The water of 3 times of quality is added into first zymotic fluid, addition mass fraction is 2%
Sugar, 100 DEG C, 30min sterilization after room temperature 8h, again carry out 100 DEG C, the sterilization of 30min fermentation medium is made；After sterilization
Inoculation quality fraction is 4% lactic acid bacteria, and 24h is left to ferment under the conditions of 30 DEG C；The lactic acid bacteria is lactobacillus plantarum and cheese
Lactobacillus Mixed Microbes, wherein, the mass ratio of lactobacillus plantarum and Lactobacillus casei is 3: 1；White sand used in the present embodiment
Sugar, can also be exchanged with the sugar such as fructose, glucose, rock sugar, its usage ratio can be converted according to contained monose；
(5) filter, sterilize：Zymotic fluid obtains supernatant liquid after diatomite filters, and pH3~4, are sterilized through 135 DEG C, 3~4s
Sprouted unpolished rice ferment drink is obtained afterwards.
As a result：The dry weight for filtering residue is less than the 25% of brown rice raw material weight, and brown rice raw material availability is higher than 75%, routine
The raw material availability that two enzymes method hydrolysis sprouted unpolished rice carries out lactobacillus-fermented is 50%~60%, by comparison the present embodiment raw material
Utilization rate greatly improves, and the cell concentration of lactic acid bacteria also improves 15%~20% by comparison during lactobacillus-fermented, is obtaining
While same active principle, fermentation time is substantially reduced.
Embodiment 2
The preparation method of the sprouted unpolished rice ferment drink of the present embodiment, includes the following steps：
(1) brown rice germination：It is raw material to select high-quality brown rice, after cleaning, disinfection, 6h is soaked in 35 DEG C of thermostatted waters, after draining
Germinate, it is 35 DEG C to keep germination environment temperature, terminates to germinate to the long 2.5mm of bud；
(2) cook：Add water according to the ratio of mass ratio 1: 2 according to sprouted unpolished rice and water, heating steam it is ripe to rice, after by its dozen
Postcooling is dissipated to room temperature；
(3) mold fermentation：The aspergillus oryzae prepared, Rhizopus oryzae are inoculated into boiling sprouted unpolished rice after cooling, 37 DEG C
Constant temperature incubation obtains the first zymotic fluid in 1 day；Will in aspergillus oryzae solid medium add with the isometric distilled water of solid medium,
Aspergillus oryzae suspension is made；Aspergillus oryzae, Rhizopus oryzae are inoculated with into the sprouted unpolished rice of boiling, total inoculum concentration is 1.5%, aspergillus oryzae suspension
Volume ratio with Rhizopus oryzae nutrient solution is 2: 1；
(4) lactobacillus-fermented：The water of 5 times of quality is added into first zymotic fluid, addition mass fraction is 6%
Sugar, 100 DEG C, 30min sterilization after room temperature 12h, again carry out 100 DEG C, the sterilization of 30min fermentation medium is made；Sterilization
Inoculation quality fraction is 6% lactic acid bacteria afterwards, and 12h is left to ferment under the conditions of 37 DEG C；The lactic acid bacteria is lactobacillus plantarum with doing
Lactobacillus paracasei Mixed Microbes, wherein, the mass ratio of lactobacillus plantarum and Lactobacillus casei is 10: 1；It is white used in the present embodiment
Granulated sugar, can also be exchanged with the sugar such as fructose, glucose, rock sugar, its usage ratio can be converted according to contained monose；
(5), filter, sterilize：Zymotic fluid obtains supernatant liquid after diatomite filters, and pH3~4, are sterilized through 135 DEG C, 3~4s
Sprouted unpolished rice ferment drink is obtained afterwards.
As a result：The dry weight for filtering residue is less than the 15% of brown rice raw material weight, and brown rice raw material availability is higher than 85%, routine
The raw material availability that two enzymes method hydrolysis sprouted unpolished rice carries out lactobacillus-fermented is 50%~60%, by comparison the present embodiment raw material
Utilization rate greatly improves, and the cell concentration of lactic acid bacteria also improves 20%~25% by comparison during lactobacillus-fermented, is obtaining
While same active principle, fermentation time is substantially reduced.
Embodiment 3
The preparation method of the sprouted unpolished rice ferment drink of the present embodiment, includes the following steps：
(1) brown rice germination：It is raw material to select high-quality brown rice, after cleaning, disinfection, 8h is soaked in 30 DEG C of thermostatted waters, after draining
Germinate, it is 32 DEG C to keep germination environment temperature, terminates to germinate to the long 2mm of bud；
(2) cook：Add water according to the ratio of mass ratio 1: 1.5 according to sprouted unpolished rice and water, heating steam it is ripe to rice, after by its
Postcooling is broken up to room temperature；
(3) mold fermentation：The aspergillus oryzae prepared, Rhizopus oryzae are inoculated into the sprouted unpolished rice of boiling, 35 DEG C of constant temperature trainings
Support 3 days and obtain the first zymotic fluid；It will be added in aspergillus oryzae solid medium and the isometric distilled water of solid medium, obtained rice
Aspergillus suspension；Aspergillus oryzae, Rhizopus oryzae are inoculated with into the sprouted unpolished rice of boiling, total inoculum concentration is 1.2%, aspergillus oryzae suspension and meter Gen
The volume ratio of mould nutrient solution is 1.5: 1；
(4) lactobacillus-fermented：The water of 4 times of quality is added into first zymotic fluid, addition mass fraction is 4%
Sugar, 100 DEG C, 30min sterilization after room temperature 10h, again carry out 100 DEG C, the sterilization of 30min fermentation medium is made；Sterilization
Inoculation quality fraction is 5% lactic acid bacteria afterwards, and 18h is left to ferment under the conditions of 35 DEG C；The lactic acid bacteria is lactobacillus plantarum with doing
Lactobacillus paracasei Mixed Microbes, wherein, the mass ratio of lactobacillus plantarum and Lactobacillus casei is 7: 1；It is white used in the present embodiment
Granulated sugar, can also be exchanged with the sugar such as fructose, glucose, rock sugar, its usage ratio can be converted according to contained monose；
(5) filter, sterilize：Zymotic fluid obtains supernatant liquid after diatomite filters, and pH3~4, are sterilized through 135 DEG C, 3~4s
Sprouted unpolished rice ferment drink is obtained afterwards.
As a result：The dry weight for filtering residue is less than the 20% of brown rice raw material weight, and brown rice raw material availability is higher than 80%, routine
The raw material availability that two enzymes method hydrolysis sprouted unpolished rice carries out lactobacillus-fermented is 50%~60%, by comparison the present embodiment raw material
Utilization rate greatly improves, and the cell concentration of lactic acid bacteria also improves 18%~20% by comparison during lactobacillus-fermented, is obtaining
While same active principle, fermentation time is substantially reduced.
Embodiment 4
The preparation method of the sprouted unpolished rice ferment drink of the present embodiment, includes the following steps：
(1) brown rice germination：It is raw material to select high-quality brown rice, after cleaning, disinfection, 10h is soaked in 33 DEG C of thermostatted waters, after draining
Germinate, it is 33 DEG C to keep germination environment temperature, terminates to germinate to the long 2mm of bud；
(2) cook：Add water according to the ratio of mass ratio 1: 1.8 according to sprouted unpolished rice and water, heating steam it is ripe to rice, after by its
Postcooling is broken up to room temperature；
(3) mold fermentation：The aspergillus oryzae prepared, Rhizopus oryzae are inoculated into the sprouted unpolished rice of boiling, 33 DEG C of constant temperature trainings
Support 2 days and obtain the first zymotic fluid；It will be added in aspergillus oryzae solid medium and the isometric distilled water of solid medium, obtained rice
Aspergillus suspension；Aspergillus oryzae, Rhizopus oryzae are inoculated with into the sprouted unpolished rice of boiling, total inoculum concentration is 1.5%, 37 DEG C of constant temperature incubations 2 days,
The volume ratio of aspergillus oryzae suspension and Rhizopus oryzae nutrient solution is 2: 1；
(4) lactobacillus-fermented：The water of 3 times of quality is added into first zymotic fluid, addition mass fraction is 5%
Sugar, 100 DEG C, 30min sterilization after room temperature 10h, again carry out 100 DEG C, the sterilization of 30min fermentation medium is made；Sterilization
Inoculation quality fraction is 6% lactic acid bacteria afterwards, and 24h is left to ferment under the conditions of 33 DEG C；The lactic acid bacteria is lactobacillus plantarum with doing
Lactobacillus paracasei Mixed Microbes, wherein, the mass ratio of lactobacillus plantarum and Lactobacillus casei is 5: 1；It is white used in the present embodiment
Granulated sugar, can also be exchanged with the sugar such as fructose, glucose, rock sugar, its usage ratio can be converted according to contained monose；
(5), filter, sterilize：Zymotic fluid obtains supernatant liquid after diatomite filters, and pH3~4, are sterilized through 135 DEG C, 3~4s
Sprouted unpolished rice ferment drink is obtained afterwards.
As a result：The dry weight for filtering residue is less than the 15% of brown rice raw material weight, and brown rice raw material availability is higher than 85%, routine
The raw material availability that two enzymes method hydrolysis sprouted unpolished rice carries out lactobacillus-fermented is 50%~60%, by comparison the present embodiment raw material
Utilization rate greatly improves, and the cell concentration of lactic acid bacteria also improves 20%~25% by comparison during lactobacillus-fermented, is obtaining
While same active principle, fermentation time is substantially reduced.
The embodiment of the present invention is described above in conjunction with attached drawing, but the invention is not limited in above-mentioned specific
Embodiment, above-mentioned embodiment is only schematical, rather than restricted, those of ordinary skill in the art
Under the enlightenment of the present invention, in the case of present inventive concept and scope of the claimed protection is not departed from, it can also make very much
Form, these are belonged within the protection of the present invention.
Claims (8)
1. a kind of preparation method of sprouted unpolished rice ferment drink, it is characterised in that include the following steps：
S100, brown rice germination：It is raw material to select high-quality brown rice, and germination is drained after being soaked in warm water, and after sprouted unpolished rice is cooked
Cooling；
S200, mold fermentation：The aspergillus oryzae prepared, Rhizopus oryzae are inoculated into the sprouted unpolished rice of boiling, 28~37 DEG C of constant temperature
Culture obtains the first zymotic fluid in 1~4 day；
S300, lactobacillus-fermented：The water of 3~5 times of quality is added into first zymotic fluid, addition mass fraction is 2~6%
Sugar, inoculation quality fraction is 4~6% lactic acid bacteria after sterilization, and 12~24h is left to ferment under the conditions of 30~37 DEG C；Filtering, institute
Obtain supernatant liquid and sprouted unpolished rice ferment drink is obtained after sterilization.
2. the preparation method of sprouted unpolished rice ferment drink according to claim 1, it is characterised in that the step S100 tools
Body includes the following steps：
S101, the high-quality brown rice of selection are raw material, after cleaning, disinfection, 6~12h are soaked in 28~35 DEG C of thermostatted waters, is carried out after draining
Germination, it is 30~35 DEG C to keep germination environment temperature, grows 1~2.5mm to bud and terminates to germinate；
S102, according to the ratio that the mass ratio of sprouted unpolished rice and water is 1: 1~1: 2 add water, heating steam it is ripe to rice, after broken up
Postcooling is to room temperature.
3. the preparation method of sprouted unpolished rice ferment drink according to claim 2, it is characterised in that the step S101
In, brown rice raw material includes at least one colored rice brown rice.
4. the preparation method of sprouted unpolished rice ferment drink according to claim 1, it is characterised in that the step S200 tools
Body includes the following steps：
S201, prepare aspergillus oryzae solid medium, Rhizopus oryzae fluid nutrient medium respectively, and sterilizing cooling is stand-by；
S202, by the aspergillus oryzae of preservation, Rhizopus oryzae actication of culture, be seeded to aspergillus oryzae solid medium, Rhizopus oryzae liquid respectively
Culture medium；30 DEG C of quiescent culture 72h of aspergillus oryzae, the during which loose culture medium of regular shaking flask；34 DEG C of Rhizopus oryzae, 200rpm shaking table cultures
60h；
S203, will add and the isometric distilled water of solid medium, obtained aspergillus oryzae suspension in aspergillus oryzae solid medium；To
It is inoculated with aspergillus oryzae, Rhizopus oryzae in the sprouted unpolished rice of boiling, total inoculum concentration is 1~1.5%, 28~37 DEG C of constant temperature incubations 1~4 day,
The volume ratio of aspergillus oryzae suspension and Rhizopus oryzae nutrient solution is 1: 1~2: 1.
5. the preparation method of sprouted unpolished rice ferment drink according to claim 1, it is characterised in that the step S300 tools
Body includes the following steps：
S301, fermentation medium prepare：The water of 3~5 times of quality is added into first zymotic fluid, addition mass fraction is 2
~6% sugar, 100 DEG C, 30min sterilization after 8~12h of room temperature, again carry out 100 DEG C, the sterilization of 30min be made fermentation training
Support base；
It is S302, inoculum fermented：In the fermentation medium inoculation quality fraction be 4~6% lactic acid bacteria, 30~37 DEG C of conditions
12~24h of lower standing for fermentation；
S303, filtering, sterilization：Supernatant liquid will be obtained after fermentation gained filtering fermentation liquor, sprouted unpolished rice ferment drink is obtained after sterilization
Product.
6. the preparation method of sprouted unpolished rice ferment drink according to claim 5, it is characterised in that the step S301
In, the sugar is at least one of white granulated sugar, fructose, glucose, rock sugar.
7. the preparation method of sprouted unpolished rice ferment drink according to claim 5, it is characterised in that the step S302
In, the lactic acid bacteria is lactobacillus plantarum and Lactobacillus casei Mixed Microbes, wherein, the quality of lactobacillus plantarum and Lactobacillus casei
Than for 3: 1~10: 1.
8. the preparation method of sprouted unpolished rice ferment drink according to claim 5, it is characterised in that the step S303
In, the zymotic fluid obtains supernatant liquid after diatomite filters, and pH3~4, sterilization conditions are 135 DEG C, 3~4s.
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Effective date of registration: 20190522 Address after: 430040 No. 5 Lingjiashan South Road, Donghu Development Zone, Wuhan City, Hubei Province Applicant after: Wuhan Sunhy Biology Co., Ltd. Address before: 344000 Huwan Town, Jinxi County, Fuzhou City, Jiangxi Province Applicant before: Jiangxi Xinhua Enzyme Technology Co., Ltd. |
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