CN102352318A - Fluid nutrient medium for promoting growth of halophilic nitrite oxidizing bacteria - Google Patents
Fluid nutrient medium for promoting growth of halophilic nitrite oxidizing bacteria Download PDFInfo
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- CN102352318A CN102352318A CN2011103245401A CN201110324540A CN102352318A CN 102352318 A CN102352318 A CN 102352318A CN 2011103245401 A CN2011103245401 A CN 2011103245401A CN 201110324540 A CN201110324540 A CN 201110324540A CN 102352318 A CN102352318 A CN 102352318A
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Abstract
A fluid nutrient medium for promoting the growth of halophilic nitrite oxidizing bacteria contains 0.3g to 0.7g of glucose, 0.6g to 1.4g of sodium nitrite, 0g to 0.04g of manganese sulfate monohydrate, 0.6g to 1.5g of potassium monohydrogen phosphate, 0.6g to 1.4g of sodium carbonate, 0g to 0.6g of potassium dihydrogen phosphate, 20g to 32g of sodium chloride, 2.8g to 3.6g of magnesium sulfate, 1.8g to 2.6g of magnesium chloride, 0.8g to 1.4g of calcium chloride, 0g to 0.6g of sodium bicarbonate, 0.3g to 1.1g of potassium chloride, 1ml to 10ml of vitamin solution, 1ml to 10ml of trace element solution and 60g to 80g of zeolite per 1000 milliliter of water, and the pH value is 8. The fluid nutrient medium is suitable for the growth of halophilic nitrite oxidizing bacteria, and can promote the growth and metabolism of halophilic nitrite oxidizing bacteria. The fluid nutrient medium provided by the invention can promote the growth of halophilic nitrite oxidizing bacteria in the fluid nutrient medium, so that the separation, purification, reproduction and denitrifying capability detection of halophilic nitrite oxidizing bacteria can become simple and feasible.
Description
Technical field
The present invention relates to salt tolerant nitrifier substratum, particularly relate to a kind of liquid nutrient medium of effective promotion salt tolerant nitrobacteria growth.
Background technology
Mostly nitrifier (Nitrite Oxidizing Bacteria) is chemosynthetic autotroph; It is the main flora of participating in rising in the biological denitrification process nitrification; Its rate of nitrification directly influences the nitrification effect of Sewage treatment systems, is the restrictive factor of bio-denitrifying sewage.And research shows that nitrifier is very sensitive to salinity; Increased the difficulty of high-salt wastewater biological treatment; Autotrophic type nitrobacteria generation time is long in addition, growth velocity is slow; Make that nitrifier is difficult in common inorganic carbon source liquid nutrient medium, grow, this salt tolerant nitrifier that makes separation of pure dissolve is difficult to enlarged culturing, in the high-salt wastewater that is difficult to be applied to carry out a biological disposal upon.The salt tolerant nitrifier grows on solid plate; But growth is very difficult in liquid; Find that through retrieval the disclosed application number of prior art is 200510111876.4 Chinese invention patent, discloses a kind of nitrobacteria culture promoter; Use after this promotor under the identical situation of other conditions, but ammonia-N removal rate improves more than 20%.Application number is that 200710032494.1 Chinese invention patent discloses a kind of substratum that improves speed of nitrite oxidation by nitrobacteria, the nitrite in the sustainable pollution degradation water body of the thalline of turning out.The liquid culture based formulas that disclosed promotion salt tolerant nitrobacteria growth is not also arranged.Therefore, promote the research and development of the liquid nutrient medium of salt tolerant nitrobacteria growth to seem particularly important.
Summary of the invention
The liquid nutrient medium that the purpose of this invention is to provide a kind of effective promotion salt tolerant nitrobacteria growth, the nitrifier substratum of realization salt tolerant nitrifier enlarged culturing.
The object of the invention is realized through following technical scheme:
A kind of liquid nutrient medium that promotes the salt tolerant nitrobacteria growth, it consists of: contain glucose 0.3-0.7g, Sodium Nitrite 0.6-1.4g, manganese sulfate monohydrate 0-0.04g, potassium phosphate,monobasic 0.6-1.5g, yellow soda ash 0.6-1.4g, potassium primary phosphate 0-0.6g, sodium-chlor 20-32g, sal epsom 2.8-3.6g, magnesium chloride 1.8-2.6g, calcium chloride 0.8-1.4g, sodium hydrogencarbonate 0-0.6g, Repone K 0.3-1.1 g, VITAMINs liquid 1-10ml, trace element solution 1-10ml, zeolite 60-80g, pH=8 in every 1000ml water.
Described VITAMINs liquid consists of: contain vitamin b23 0.01-0.02g, xitix 0.02-0.03g, nuclear in every 1000ml water
Flavine 0.02-0.03g, Hydrocerol A 0.02-0.03g, pyridoxal hydrochloride 0.04-0.06g, folic acid 0.01-0.02g, para-amino benzoic acid 0.01-0.02g, creatine 0.02-0.04g.
Consisting of of described trace element solution: contain EDTA Disodium 63-64g, zinc sulfate in every 1000ml water
2-2.5g, four hydration Manganous chloride tetrahydrate 4.9-5.1g, seven ferric sulfate hydrate 4.9-5.1g, four molybdic acid hydrate disodium 1-1.2g, Salzburg vitriol 1.4-1.6g, cobalt chloride hexahydrate 1.5-1.7g, pH=7.
Be further to realize the object of the invention, contain said glucose in every 1000ml water and be preferably 0.4-0.6g, Sodium Nitrite and be preferably 0.8-1.2g, manganese sulfate monohydrate and be preferably 0.05-0.02g, potassium phosphate,monobasic and be preferably 0.8-1.2g, yellow soda ash and be preferably 0.8-1.2g, potassium primary phosphate and be preferably 0.1-0.4g, sodium-chlor and be preferably 24-38g, sal epsom and be preferably 3-3.4g, magnesium chloride and be preferably 2-2.4g, calcium chloride and be preferably 1-1.3g, sodium hydrogencarbonate and be preferably 0.1-0.4g, Repone K and be preferably 0.5-0.9 g, VITAMINs liquid and be preferably that 1-5ml, trace element solution are preferably 1-5ml, zeolite is preferably 65-85g, pH=8.
For further realizing the object of the invention, the substratum composition is preferably and contains said glucose 0.5g, Sodium Nitrite 1g, Manganous sulfate monohydrate 0.01g, potassium phosphate,monobasic 0.1g, yellow soda ash 1g, potassium primary phosphate 0.3g, sodium-chlor 26.7g, sal epsom 3.3g, magnesium chloride 2.3g, calcium chloride 1.2g, sodium hydrogencarbonate 0.2, Repone K 0.75g, VITAMINs liquid 2ml, trace element solution 2ml, zeolite 70g, pH=8 in every 1000ml water.
The liquid nutrient medium of promotion salt tolerant nitrobacteria growth provided by the invention mainly contains three parts, and glucose wherein is rich in dissolved organic carbon, macromolecular substance, and VITAMINs liquid is rich in abundant VITAMINs, can keep salt tolerant nitrifier activity.It two is sodium hydrogencarbonate, potassium phosphate,monobasic, potassium primary phosphate, and its effect is to form buffered soln, regulates the pH of substratum, makes that nitrifier can grow fast under ph optimum.It three is a zeolite, and its effect is for nitrifier provides growing carrier, utilizes nitrifier to be prone to be attached to the solid surface growth, is prone to form the character that macrobead sinks to container bottom, promotes that nitrifier grows fast.
Advantage of the present invention mainly is to have solved the problem that it is difficult that the salt tolerant nitrifier grows in the salt-containing liquid substratum, for the effect of the oxidation nitrous acid of investigating the salt tolerant nitrifier and the separation and purification and the enlarged culturing of pure strain provide an approach.
Embodiment
Below in conjunction with embodiment the present invention is further explained.
Embodiment 1
In 1000ml water, add glucose 0.3g, Sodium Nitrite 0.6, manganese sulfate monohydrate 0g, potassium phosphate,monobasic 0.6g, yellow soda ash 0.6g, potassium primary phosphate 0, sodium-chlor 20g, sal epsom 2.8g, magnesium chloride 1.8g, calcium chloride 0.8g, sodium hydrogencarbonate 0g, Repone K 0.3 g, VITAMINs liquid 1ml, trace element solution 1ml, zeolite 60g, pH=8 successively.
With the nitrifier bacterial classification, the inoculum size by 10% inserts above-mentioned substratum, 30 ℃ of temperature, constant-temperature shaking culture.Behind the inoculation 24h, the bacterium of nitrifier is turbid to be significantly improved; And with batch being inoculated into the substratum that does not add glucose, VITAMINs liquid, zeolite, bacterium is turbid does not improve, and descends slightly.The result shows that liquid nutrient medium provided by the invention is fit to the salt tolerant nitrobacteria growth.
Embodiment 2
In 1000ml water, add glucose 0.7g, Sodium Nitrite 1.4g, manganese sulfate monohydrate 0.04g, potassium phosphate,monobasic 1.5g, yellow soda ash 1.4g, potassium primary phosphate 0.6g, sodium-chlor 32g, sal epsom 3.6g, magnesium chloride 2.6g, calcium chloride 1.4g, sodium hydrogencarbonate 0.6g, Repone K 1.1 g, VITAMINs liquid 10ml, trace element solution 10ml, zeolite 80g, pH=8 successively.
With the nitrifier bacterial classification, the inoculum size by 10% inserts above-mentioned substratum, 30 ℃ of temperature, constant-temperature shaking culture.Behind the inoculation 12h, the bacterium of nitrifier is turbid to be significantly improved; And with batch being inoculated into the substratum that does not add glucose, VITAMINs liquid, zeolite, bacterium is turbid does not improve, and descends slightly.The result shows that liquid nutrient medium provided by the invention has promoted the salt tolerant nitrobacteria growth.
Embodiment 3
In 1000ml water, add glucose 0.5g, Sodium Nitrite 1g, Manganous sulfate monohydrate 0.01g, potassium phosphate,monobasic 1g, yellow soda ash 1g, potassium primary phosphate 0.3g, sodium-chlor 26.7g, sal epsom 3.3g, magnesium chloride 2.3g, calcium chloride 1.2g, sodium hydrogencarbonate 0.2g, Repone K 0.75g, VITAMINs liquid 2ml, trace element solution 2ml, zeolite 70g, pH=8 successively.
With the nitrifier bacterial classification, the inoculum size by 10% inserts above-mentioned substratum, 30 ℃ of temperature, constant-temperature shaking culture.Behind the inoculation 15h, the bacterium of nitrifier is turbid to be significantly improved; And with batch being inoculated into the substratum that does not add glucose, VITAMINs liquid, zeolite, bacterium is turbid does not improve, and descends slightly.Process is tested, and compares with comparative group to show that liquid nutrient medium provided by the invention has promoted the salt tolerant nitrobacteria growth.
Claims (5)
1. liquid nutrient medium that promotes the salt tolerant nitrobacteria growth; It is characterized in that this substratum consists of: contain glucose 0.3-0.7g, Sodium Nitrite 0.6-1.4g, Manganous sulfate monohydrate 0-0.04g, potassium phosphate,monobasic 0.6-1.5g, yellow soda ash 0.6-1.4g, potassium primary phosphate 0-0.6g, sodium-chlor 20-32g, sal epsom 2.8-3.6g, magnesium chloride 1.8-2.6g, calcium chloride 0.8-1.4g, sodium hydrogencarbonate 0-0.6g, Repone K 0.3-1.1 g, VITAMINs liquid 1-10ml, trace element solution 1-10ml, zeolite 60-80g, pH=8 in every 1000ml water.
2. the liquid nutrient medium of promotion salt tolerant nitrobacteria growth according to claim 1 is characterized in that: contain glucose 0.4-0.6g, Sodium Nitrite 0.8-1.2g, manganese sulfate monohydrate 0.05-0.02g, potassium phosphate,monobasic 0.8-1.2g, yellow soda ash 0.8-1.2g, potassium primary phosphate 0.1-0.4g, sodium-chlor 24-38g, sal epsom 3-3.4g, magnesium chloride 2-2.4g, calcium chloride 1-1.3g, sodium hydrogencarbonate 0.1-0.4g, Repone K 0.5-0.9 g, VITAMINs liquid 1-5ml, trace element solution 1-5ml, zeolite 65-85g, pH=8 in every 1000ml water.
3. according to liquid nutrient medium, it is characterized in that: contain described glucose 0.5g, Sodium Nitrite 1g, Manganous sulfate monohydrate 0.01g, potassium phosphate,monobasic 1g, yellow soda ash 1g, potassium primary phosphate 0.3g, sodium-chlor 26.7g, sal epsom 3.3g, magnesium chloride 2.3g, calcium chloride 1.2g, sodium hydrogencarbonate 0.2g, Repone K 0.75g, VITAMINs liquid 2ml, trace element solution 2ml, zeolite 70g, pH=8 in every 1000ml water by the described promotion salt tolerant of claim 1 nitrobacteria growth.
4. according to the liquid nutrient medium of any one described promotion salt tolerant nitrobacteria growth among the claim 1-3, it is characterized in that: described VITAMINs liquid consists of: contain vitamin b23 0.01-0.02g, xitix 0.02-0.03g, vitamin G 0.02-0.03g, Hydrocerol A 0.02-0.03g, pyridoxal hydrochloride 0.04-0.06g, folic acid 0.01-0.02g, para-amino benzoic acid 0.01-0.02g, creatine 0.02-0.04g in every 1000ml water.
5. according to the liquid nutrient medium of any one described promotion salt tolerant nitrobacteria growth of claim 1-3, it is characterized in that: the consisting of of described trace element solution: contain EDTA Disodium 63-64g, zinc sulfate 2-2.5g, four hydration Manganous chloride tetrahydrate 4.9-5.1g, seven ferric sulfate hydrate 4.9-5.1g, four molybdic acid hydrate disodium 1-1.2g, Salzburg vitriol 1.4-1.6g, cobalt chloride hexahydrate 1.5-1.7g, pH=7 in every 1000ml water.
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| CN102628025A (en) * | 2012-04-09 | 2012-08-08 | 天津大学 | Nutrient liquid formula for culturing high-temperature thermophilic bacterium and preparation method and application thereof |
| CN103589669A (en) * | 2013-10-27 | 2014-02-19 | 沈阳建筑大学 | Salt-resistant denitriding composite inoculant, and preparation method and use method thereof |
| CN104711213A (en) * | 2015-03-13 | 2015-06-17 | 中国海洋石油总公司 | Accelerant facilitating growth of salt-resistive nitrifying bacteria in high-salt wastewater |
| CN106474914A (en) * | 2016-11-11 | 2017-03-08 | 云南大学 | Improve the orientation biological reinforcing method of simultanously desulfurizing and denitrification biomembrane filled tower nitric efficiency |
| CN108103000A (en) * | 2017-12-27 | 2018-06-01 | 齐鲁工业大学 | A kind of growth promoter for improving anaerobic ammonia oxidizing bacteria and preparation method thereof |
| CN111099722A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for promoting denitrification and application thereof |
| CN111676212A (en) * | 2020-06-15 | 2020-09-18 | 深圳市瑞赛生物技术有限公司 | Culture method and kit for quantitatively detecting vitamins by microorganisms |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102628025A (en) * | 2012-04-09 | 2012-08-08 | 天津大学 | Nutrient liquid formula for culturing high-temperature thermophilic bacterium and preparation method and application thereof |
| CN103589669A (en) * | 2013-10-27 | 2014-02-19 | 沈阳建筑大学 | Salt-resistant denitriding composite inoculant, and preparation method and use method thereof |
| CN104711213A (en) * | 2015-03-13 | 2015-06-17 | 中国海洋石油总公司 | Accelerant facilitating growth of salt-resistive nitrifying bacteria in high-salt wastewater |
| CN106474914A (en) * | 2016-11-11 | 2017-03-08 | 云南大学 | Improve the orientation biological reinforcing method of simultanously desulfurizing and denitrification biomembrane filled tower nitric efficiency |
| CN106474914B (en) * | 2016-11-11 | 2019-05-24 | 云南大学 | Improve the orientation biological reinforcing method of simultanously desulfurizing and denitrification biomembrane filled tower nitric efficiency |
| CN108103000A (en) * | 2017-12-27 | 2018-06-01 | 齐鲁工业大学 | A kind of growth promoter for improving anaerobic ammonia oxidizing bacteria and preparation method thereof |
| CN111099722A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for promoting denitrification and application thereof |
| CN111676212A (en) * | 2020-06-15 | 2020-09-18 | 深圳市瑞赛生物技术有限公司 | Culture method and kit for quantitatively detecting vitamins by microorganisms |
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Application publication date: 20120215 |