CN101186888A - Culture medium for increasing speed of nitrite oxidation by nitrobacteria - Google Patents
Culture medium for increasing speed of nitrite oxidation by nitrobacteria Download PDFInfo
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- CN101186888A CN101186888A CNA2007100324941A CN200710032494A CN101186888A CN 101186888 A CN101186888 A CN 101186888A CN A2007100324941 A CNA2007100324941 A CN A2007100324941A CN 200710032494 A CN200710032494 A CN 200710032494A CN 101186888 A CN101186888 A CN 101186888A
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Abstract
The invention discloses a culture medium for improving the speed of nitrifying bacteria oxidizing nitrites. The composition of the culture medium is that every 1000 milliliters of water contain 2.1 to 3.1 grams of glycerin, 1.3 to 1.9 grams of yeast extract, 1.0 to 2.0 grams of baking soda,1.9 to 3.2 grams of sodium nitrite, 0.1 to 0.8 grams of sodium carbonate, 0.1 to 0.5 grams of sodium chloride, 0.1 to 0.5 grams of potassium dihydrogen phosphate, 0.1 to 0.5 grams of magnesium sulfate and 0.005 to 0.05 grams of ferrous sulfate, wherein the glycerin and the yeast extract are better to be supplemented at the late stage of fermentation. The culture medium ensures obvious promotion of the speed of growth metabolism of the nitrifying bacteria and significant increasing of the degradation rate of the nitrites, thereby the average degradation rate of the invention is increased 40% to 127% in comparison with a conventional culture medium 900-1320mgNO2-N/gMLSS.d. Tests of small water body and real water body prove that bacteria cultured by the invention can continuously and high effectively degrade nitrites in polluted water body.
Description
Technical field
The present invention relates to the nitrifier fermention medium, particularly relate to a kind of substratum of effective raising speed of nitrite oxidation by nitrobacteria.
Background technology
Nitrifier (Nitrite Oxidizing Bacteria) is a class important microbe of nitrate for degrading nitrite in the nature nitrogen cycle.Because the water body micro-ecological environment has suffered havoc in recent years, waters big area nitrite excessively accumulates poisoning and frequently breaks out, and has directly or indirectly caused the massive losses of China's economy.Yet the nitrifier product is most deficient on the market in China, mostly is the substitute products of other kind poor efficiency mixing microorganismss greatly, as photosynthetic bacterium, genus bacillus etc.Especially with special disappearance at the research of eutrophication water nitrite degradation.Therefore, nitrifier Research of Microorganisms Agent and exploitation seem particularly important.
The disclosed conventional nitrifier substratum of prior art is: the substratum that use South China Science ﹠ Engineering University microbe research chamber.Contain sodium bicarbonate 2.0g/L in every 1000mL water, Sodium Nitrite 0.5g/L, potassium primary phosphate 0.2g/L, sal epsom 0.05g/L, ferric sulfate 0.1g/L.Conventional nitrifier substratum since component with difference of the present invention, key enzyme---nitrite-oxidizing reductase enzyme that can't the quick active nitrifier is so that nitrifier reaches best degrading nitrite state; Simultaneously, owing to lack the organic substrates of necessary content, under the normal temperature anaerobic environment, the decay rate of death of nitrifier and the deactivation rate of nitrite-oxidizing reductase enzyme have been accelerated on the contrary.
Summary of the invention
The substratum that the purpose of this invention is to provide a kind of effective raising speed of nitrite oxidation by nitrobacteria, the nitrifier substratum of realization nitrifier live bacteria agent scale operation.
Purpose of the present invention is achieved through the following technical solutions:
A kind of substratum that improves speed of nitrite oxidation by nitrobacteria, it consists of: contain glycerine 2.1-3.1g, yeast extract 1.3-1.9g, sodium bicarbonate 1.0-2.0g, Sodium Nitrite 1.9-3.2g, yellow soda ash 0.1-0.8g, sodium-chlor 0.1-0.5g, potassium primary phosphate 0.1-0.5g, sal epsom 0.1-0.5g and ferrous sulfate 0.005-0.05g in every 1000mL water.
For further realizing containing the object of the invention described glycerine in every 1000mL water and be preferably 2.5-2.8g.
Described glycerine is to add in the substratum in earlier fermentation or fermentation later stage, and the fermentation later stage adds better effects if.
Contain described yeast extract in every 1000mL water and be preferably 1.5-1.8g.
Described yeast extract is that earlier fermentation adds or the fermentation later stage adds in the substratum, and the fermentation later stage adds better effects if.
Contain described sodium bicarbonate in every 1000mL water and be preferably 1.5-1.8g, Sodium Nitrite is preferably 2.1-2.8g, and yellow soda ash is preferably 0.4-0.6g.Sodium-chlor is preferably that 0.3-0.4g, potassium primary phosphate are preferably 0.3-0.5g, sal epsom is preferably 0.2-0.3g and ferrous sulfate is preferably 0.008-0.02g.
For further realizing the object of the invention, the substratum composition is preferably and contains described glycerine 2.6g, yeast extract 1.6g, sodium bicarbonate 1.7g, Sodium Nitrite 2.7g, yellow soda ash 0.5g, sodium-chlor 0.4g, potassium primary phosphate 0.4g, sal epsom 0.2g and ferrous sulfate 0.01g in every 1000mL water.
The present invention utilizes nitrifier can grow fast under aerobic environment, under anaerobic environment, can utilize organism to keep the characteristics of degrading activity, obtains the culture medium prescription an of the best by optimization.It has been generally acknowledged that nitrifier can utilize inorganic medium to grow fast under aerobic environment.Sink to reactor bottom but the nitrifier thalline easily flocculates into macrobead,, can make nitrifier under anaerobic environment, continue to keep activity because the relative anoxic of reactor bottom is added organism such as glycerine and yeast extract by the later stage.
According to formulated of the present invention (glycerine and yeast extract can ferment later stage add), the inoculum size with 10% inserts nitrifier, regulates air flow simultaneously with substratum in the present invention, so that dissolved oxygen is greater than 2mg/L, temperature is 25-30 ℃.After cultivating 9d, can stop ventilation, precipitate the supernatant liquor that inclines, be concentrated into desired concn and get final product.
With respect to prior art, the present invention has following advantage and beneficial effect:
(1) provide the prescription of growing fast in the nitrifier aerobic environment, this prescription is significantly accelerated the growth metabolism speed of nitrifier; Degradation rate to nitrite significantly improves, and average degradation rate improves 40%-60% than conventional substratum, reaches 850-1600mgNO2-N/gMLSSd, and general degradation rate is stabilized in 950-1100mgNO2-N/gMLSSd.Little water body and true water body evidence, the nitrite in the sustainable efficient degradation polluted-water of the thalline that the present invention turns out.
(2) solved thalline in the culturing process owing to self assemble flocculation precipitation reaction device bottom, in relative anaerobic environment, the problem that the loss of nitrifier degrading activity is too fast.
Description of drawings
Fig. 1 is nitrite concentration result of variations figure in the water of the interior grow shrimp pool of different time.
Embodiment
The present invention is further illustrated below in conjunction with embodiment, need to prove, these embodiment do not constitute limiting the scope of the invention.
-In 1000mL water, dissolve glycerine 2.1, yeast extract 1.3g, sodium bicarbonate 1.0g, Sodium Nitrite 1.9g, yellow soda ash 0.1g, sodium-chlor 0.1g, potassium primary phosphate 0.1g, sal epsom 0.1g and ferrous sulfate 0.005g successively and make substratum.
Nitrifier bacterial classification (Nitrobacter hamburgensis, Nitrobacter winogradskyi etc.) activation also is amplified to 1L step by step, 30 ℃ of temperature, dissolved oxygen is controlled greater than 5.0mgO2/L, and by volume the inoculum size of per-cent 10% inserts above-mentioned substratum.Cultured microbial inoculum records degradation rate 926.4mgNO2-N/gMLSSd.(contain sodium bicarbonate 2.0g/L in every 1000mL water, Sodium Nitrite 0.5g/L, potassium primary phosphate 0.2g/L, sal epsom 0.05g/L, ferric sulfate 0.1g/L with batch conventional substratum of use.) obtain degradation rate 580.0mgNO
2-N/gMLSSd.The result shows that the nitrifier degradation rate that the present invention cultivates has improved 59.7% than conventional substratum.
Embodiment 2
In 1000mL water, dissolve glycerine 3.1, yeast extract 1.9g, sodium bicarbonate 2.0g, Sodium Nitrite 3.2g, yellow soda ash 0.8g, sodium-chlor 0.5g, potassium primary phosphate 0.5g, sal epsom 0.5g and ferrous sulfate 0.05g successively and make substratum.Nitrifier bacterial classification (Nitrobacter hamburgensis, Nitrobacter winogradskyi etc.) activation also is amplified to 1L step by step, 28 ℃ of temperature, dissolved oxygen is controlled greater than 5.0mgO2/L, and by volume the inoculum size of per-cent 5% inserts above-mentioned substratum.Cultured microbial inoculum records degradation rate 947.8mgNO2-N/gMLSSd.(contain sodium bicarbonate 2.0g/L in every 1000mL water, Sodium Nitrite 0.5g/L, potassium primary phosphate 0.2g/L, sal epsom 0.05g/L, ferric sulfate 0.1g/L with batch conventional substratum of use.) obtain degradation rate 580.0mgNO
2-N/gMLSSd.The result shows that the nitrifier degradation rate that the present invention cultivates has improved 63.4% than conventional substratum.
Dissolve glycerine 2.6g in 1000mL water successively, yeast extract 1.6g, sodium bicarbonate 1.7g, Sodium Nitrite 2.7g, yellow soda ash 0.5g, sodium-chlor 0.4g, potassium primary phosphate 0.4g, sal epsom 0.2g and ferrous sulfate 0.01g make substratum.Nitrifier bacterial classification (Nitrobacter hamburgensis, Nitrobacter winogradskyi etc.) activation also is amplified to 1L step by step, 32 ℃ of temperature, dissolved oxygen is controlled greater than 5.0mg/L, and by volume the inoculum size of per-cent 8% inserts above-mentioned substratum.Cultured microbial inoculum records degradation rate 1194.1mgNO2-N/gMLSSd.(contain sodium bicarbonate 2.0g/L in every 1000mL water, Sodium Nitrite 0.5g/L, potassium primary phosphate 0.2g/L, sal epsom 0.05g/L, ferric sulfate 0.1g/L with batch conventional substratum of use.) obtain degradation rate 580.0mgNO
2-N/gMLSSd.The result shows that the nitrifier degradation rate that the present invention cultivates has improved 105.9% than conventional substratum.
In 1000mL water, dissolve sodium bicarbonate 1.7g, Sodium Nitrite 2.7g, yellow soda ash 0.5g, sodium-chlor 0.4g, potassium primary phosphate 0.4g, sal epsom 0.2g and ferrous sulfate 0.01g successively.
Nitrifier bacterial classification (Nitrobacter hamburgensis, Nitrobacter winogradskyi etc.) activation also is amplified to 1L step by step, 30 ℃ of temperature, dissolved oxygen is controlled greater than 5.0mg/L, and by volume the inoculum size of per-cent 10% inserts substratum.Enter the fermentation later stage behind the 2d, add glycerine 2.6, yeast extract 1.6g.Cultured microbial inoculum records degradation rate 1310.5mgNO2-N/gMLSSd.(contain sodium bicarbonate 2.0g/L in every 1000mL water, Sodium Nitrite 0.5g/L, potassium primary phosphate 0.2g/L, sal epsom 0.05g/L, ferric sulfate 0.1g/L with batch conventional substratum of use.) obtain degradation rate 576.6mgNO2-N/gMLSSd.
The result obviously as seen, the nitrifier degradation rate that the present invention cultivates has improved 127.3% than conventional substratum.
The Nitrobacter winogradskyi nitrifier microbial inoculum of being turned out with embodiment 1, (consumption of biomass=1.99gVSS/L) is directly thrown and is spilled into true water body by every mu of (degree of depth is in 1 meter) 1L.In the experimentation, the oxygenating machine distribution of control group and experimental group, aeration time, throw something and feed feed time and consumption are all consistent, and do not add any other microbiobacterial agent and chemical reagent during the course.
The result is as shown in Figure 1: in three days, add 3 experimental group of Nitrobacter winogradskyi nitrifier microbial inoculum, the nitrite starting point concentration respectively by 1.35,1.01 and 1.84mg/L drop to 0.03,0.01 and 0.00mg/L.After this maintain safe nitrite concentration following (0.2mg/L).And 3 control groups that do not add microbial inoculum, nitrite concentration by 1.23,0.98 and 1.68mg/L rise to 2.71,2.54 and 2.95mg/L respectively, and have and continue the trend that increases.As seen, add nitrifier and reached the effect that in water body, continues effectively degrading nitrite.
Claims (10)
1. substratum that improves speed of nitrite oxidation by nitrobacteria, it is characterized in that this substratum consists of: contain glycerine 2.1-3.1g, yeast extract 1.3-1.9g, sodium bicarbonate 1.0-2.0g, Sodium Nitrite 1.9-3.2g, yellow soda ash 0.1-0.8g, sodium-chlor 0.1-0.5g, potassium primary phosphate 0.1-0.5g, sal epsom 0.1-0.5g and ferrous sulfate 0.005-0.05g in every 1000mL water.
2. according to substratum, it is characterized in that containing described glycerine in every 1000mL water is 2.5-2.8g by the described raising speed of nitrite oxidation by nitrobacteria of claim 1.
3. according to substratum, it is characterized in that described glycerine is to add in the substratum in earlier fermentation or fermentation later stage by claim 1 or 2 described raising speed of nitrite oxidation by nitrobacteria.
4. according to substratum, it is characterized in that containing in every 1000mL water described yeast extract 1.5-1.8g by the described raising speed of nitrite oxidation by nitrobacteria of claim 1.
5. according to substratum, it is characterized in that described yeast extract is that earlier fermentation adds or the fermentation later stage adds in the substratum by claim 1 or 3 described raising speed of nitrite oxidation by nitrobacteria.
6. according to substratum, it is characterized in that containing in every 1000mL water described sodium bicarbonate 1.5-1.8g by the described raising speed of nitrite oxidation by nitrobacteria of claim 1.
7. according to substratum, it is characterized in that containing in every 1000mL water described Sodium Nitrite 2.1-2.8g by the described raising speed of nitrite oxidation by nitrobacteria of claim 1.
8. according to substratum, it is characterized in that containing in every 1000mL water described yellow soda ash 0.4-0.6g by the described raising speed of nitrite oxidation by nitrobacteria of claim 1.
9. according to substratum, it is characterized in that containing in every 1000mL water described sodium-chlor 0.3-0.4g, potassium primary phosphate 0.3-0.5g, sal epsom 0.2-0.3g and ferrous sulfate 0.008-0.02g by the described raising speed of nitrite oxidation by nitrobacteria of claim 1.
10. according to substratum by the described raising speed of nitrite oxidation by nitrobacteria of claim 1, it is characterized in that containing in every 1000mL water described glycerine 2.6g, yeast extract 1.6g, sodium bicarbonate 1.7g, Sodium Nitrite 2.7g, yellow soda ash 0.5g, sodium-chlor 0.4g, potassium primary phosphate 0.4g, sal epsom 0.2g and ferrous sulfate 0.01g.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352318A (en) * | 2011-10-24 | 2012-02-15 | 沈阳建筑大学 | Fluid nutrient medium for promoting growth of halophilic nitrite oxidizing bacteria |
| CN108611289A (en) * | 2016-12-09 | 2018-10-02 | 中国科学院生态环境研究中心 | A kind of cultural method and its special culture media of nitrobacteria |
| CN110627228A (en) * | 2019-11-12 | 2019-12-31 | 天津软银科技有限公司 | Composition for nitrobacteria culture |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100448983C (en) * | 2005-12-22 | 2009-01-07 | 中国石化上海石油化工股份有限公司 | Nitrobacteria growth promoter |
-
2007
- 2007-12-14 CN CN200710032494A patent/CN101186888B/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352318A (en) * | 2011-10-24 | 2012-02-15 | 沈阳建筑大学 | Fluid nutrient medium for promoting growth of halophilic nitrite oxidizing bacteria |
| CN108611289A (en) * | 2016-12-09 | 2018-10-02 | 中国科学院生态环境研究中心 | A kind of cultural method and its special culture media of nitrobacteria |
| CN110627228A (en) * | 2019-11-12 | 2019-12-31 | 天津软银科技有限公司 | Composition for nitrobacteria culture |
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