CN110627228A - Composition for nitrobacteria culture - Google Patents
Composition for nitrobacteria culture Download PDFInfo
- Publication number
- CN110627228A CN110627228A CN201911091257.1A CN201911091257A CN110627228A CN 110627228 A CN110627228 A CN 110627228A CN 201911091257 A CN201911091257 A CN 201911091257A CN 110627228 A CN110627228 A CN 110627228A
- Authority
- CN
- China
- Prior art keywords
- weight
- composition
- parts
- sodium molybdate
- manganese chloride
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention provides a composition for culturing nitrobacteria, which is mainly applied to water environment treatment and consists of sodium molybdate dihydrate, manganese chloride tetrahydrate, a yeast culture and an adsorbent.
Description
Technical Field
The invention relates to the field of sewage treatment, and in particular relates to a composition for culturing nitrobacteria.
Background
Ammonia nitrogen is a great factor of water body pollution and is not easy to remove, and the ammonia nitrogen is most commonly removed by a microbial degradation method, especially by nitrifying bacteria in municipal sewage and industrial sewage treatment at present. The biological method for removing ammonia nitrogen mainly comprises a nitrification process and a denitrification process. In the nitrification process, ammonia nitrogen is oxidized into nitrate or nitrite nitrogen under the action of nitrifying bacteria under the aerobic condition; denitrification refers to the process by which nitrate and nitrite are reduced to gaseous nitrogen. Under the anoxic condition, the denitrifying bacteria reduce nitrate and nitrite into nitrogen by using organic matters as electron donors. In the using process, the adding amount of nitrobacteria is large, and the water purification period is long.
Disclosure of Invention
The invention provides a composition for culturing nitrobacteria, which can build an environment suitable for the growth and propagation of the nitrobacteria in a water body, so that the nitrobacteria can grow and propagate in a short time, and the time required by degrading ammonia nitrogen by a biological method is shortened.
A composition for nitrobacteria culture, the composition comprising: (calcium chloride, magnesium sulfate heptahydrate, ethylene diamine tetraacetic acid, sodium molybdate dihydrate, manganese chloride tetrahydrate, cobalt chloride hexahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, ammonium sulfate and potassium hydrogen phosphate), sodium molybdate dihydrate, manganese chloride tetrahydrate, a yeast culture and an adsorbent. The mixture ratio is as follows:
sodium molybdate dihydrate: 0.5 to 2 parts by weight
Manganese chloride tetrahydrate: 0.01-0.5 weight part
Yeast culture: 15-30 parts by weight
Adsorbent: 800-
The adsorbent comprises one or more of montmorillonite, zeolite powder, and calcium carbonate.
In the above mixture ratio, the optimal mixture ratio of the sodium molybdate dihydrate is 1 part by weight; the optimal proportion of the manganese chloride tetrahydrate is 0.02 part by weight; the optimal proportion of the yeast culture is 20 parts by weight; the best mixture ratio of the adsorbent is 979.98 parts.
The nitrifying bacteria are very sensitive to the environment, the growth and the propagation of the nitrifying bacteria are very dependent on the environment, the yeast culture is rich in soluble organic matters, vitamins, sodium molybdate dihydrate and manganese chloride tetrahydrate supplement essential mineral matters for the growth of the nitrifying bacteria, the essential mineral matters are used as enzyme components and participate in a nitration reaction, and the adsorbent provides a growth carrier for the growth of the nitrifying bacteria.
Detailed Description
Example 1
Sodium molybdate dihydrate: 1 part by weight
Manganese chloride tetrahydrate: 0.02 parts by weight
Yeast culture: 20 parts by weight of
Montmorillonite: 979.98 parts by weight
Mixing the above components uniformly to obtain the mixture.
Example 2
Sodium molybdate dihydrate: 0.8 part by weight
Manganese chloride tetrahydrate: 0.01 part by weight
Yeast culture: 18 parts by weight
Zeolite powder, calcium carbonate: 800 parts by weight
Mixing the above components uniformly to obtain the mixture.
Example 3
Sodium molybdate dihydrate: 1.6 parts by weight
Manganese chloride tetrahydrate: 0.3 part by weight
Yeast culture: 25 parts by weight of
Montmorillonite, calcium carbonate: 1000 parts by weight
Mixing the above components uniformly to obtain the mixture.
The compositions formulated in the above embodiments were tested using the following methods:
the area of the test is pond sewage, the main pollution source is domestic garbage, and the COD, BOD and ammonia nitrogen of the water quality are measured.
Taking 4m of sewage from the pond for carrying out thin-wall labor, and carrying out thin-wall labor and thin-wall labor respectively, wherein the thin-wall labor and the thin-wall labor are divided into a control group, a test 1 group, a test 2 group and a test 3 group, and each group carries out thin-wall labor and thin-wall;
carrying out water sample collection and detection 24 hours after nitrobacteria are put into the control group according to 2g/m for carrying out the ethanol fermentation;
test 1 group, namely adding 20g of the composition in the embodiment 1 after nitrobacteria are put into the group according to 2g/m, and collecting a water sample for detection within 24 hours;
2 groups of experiments are carried out, 20g of the composition in the embodiment 2 of the invention is added at the same time after 2g/m of nitrobacteria are put into the ethanol, and water sample collection and detection are carried out for 24 hours;
test 3 groups, namely adding 20g of the composition in the embodiment 3 after nitrifying bacteria are put into the group according to 2g/m, and collecting a water sample for detection within 24 hours;
the test results are shown in Table 1
TABLE 1
| CODCR mg/L | BOD5 mg/L | Ammonia nitrogen (calculated as N) mg/L | |
| Before treatment | 685 | 346 | 9.12 |
| Control group | 114 | 53.9 | 1.72 |
| Test 1 group | 47 | 9.5 | 0.30 |
| Test 2 groups | 59 | 11.7 | 0.92 |
| Test 3 groups | 86 | 34.8 | 1.01 |
Claims (4)
1. The composition for culturing nitrobacteria is characterized by comprising sodium molybdate dihydrate, manganese chloride tetrahydrate, yeast culture and adsorbent, and the mixture ratio is as follows:
sodium molybdate dihydrate: 0.5 to 2 parts by weight
Manganese chloride tetrahydrate: 0.01-0.5 weight part
Yeast culture: 15-30 parts by weight
Adsorbent: 800-
The adsorbent is one or more of montmorillonite, zeolite powder, and calcium carbonate.
2. The composition of claim 1, wherein the manganese chloride tetrahydrate is in the range of 0.01 to 0.3 parts by weight.
3. The composition of claim 1, wherein the amount of sodium molybdate dihydrate is 0.8 to 1.6 parts by weight.
4. The composition according to claim 1, wherein the proportion of the yeast culture is 18 to 25 parts by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911091257.1A CN110627228A (en) | 2019-11-12 | 2019-11-12 | Composition for nitrobacteria culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911091257.1A CN110627228A (en) | 2019-11-12 | 2019-11-12 | Composition for nitrobacteria culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110627228A true CN110627228A (en) | 2019-12-31 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911091257.1A Pending CN110627228A (en) | 2019-11-12 | 2019-11-12 | Composition for nitrobacteria culture |
Country Status (1)
| Country | Link |
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| CN (1) | CN110627228A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1986445A (en) * | 2005-12-22 | 2007-06-27 | 中国石化上海石油化工股份有限公司 | Nitrobacteria culture promoter |
| CN101186888A (en) * | 2007-12-14 | 2008-05-28 | 华南理工大学 | Culture medium for increasing speed of nitrite oxidation by nitrobacteria |
| CN101792715A (en) * | 2010-01-22 | 2010-08-04 | 北京桑德环保集团有限公司 | Nitrifying bacterial agent and preparation method thereof |
| CN102337232A (en) * | 2011-04-13 | 2012-02-01 | 吉林农业大学 | Aerobic denitrification psychrotolerant bacterium and preparation method thereof |
| CN104548921A (en) * | 2014-12-23 | 2015-04-29 | 北京伊普国际水务有限公司 | Biological deodorization filling material and preparation method thereof |
| CN105624095A (en) * | 2014-10-28 | 2016-06-01 | 中国石油化工股份有限公司 | Enrichment culture method for nitrifying bacteria by waste water generated in catalyst production process |
| CN107603909A (en) * | 2017-09-30 | 2018-01-19 | 河北天泓环保科技有限公司 | Sewage treatment microbial inoculant and its application method |
-
2019
- 2019-11-12 CN CN201911091257.1A patent/CN110627228A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1986445A (en) * | 2005-12-22 | 2007-06-27 | 中国石化上海石油化工股份有限公司 | Nitrobacteria culture promoter |
| CN101186888A (en) * | 2007-12-14 | 2008-05-28 | 华南理工大学 | Culture medium for increasing speed of nitrite oxidation by nitrobacteria |
| CN101792715A (en) * | 2010-01-22 | 2010-08-04 | 北京桑德环保集团有限公司 | Nitrifying bacterial agent and preparation method thereof |
| CN102337232A (en) * | 2011-04-13 | 2012-02-01 | 吉林农业大学 | Aerobic denitrification psychrotolerant bacterium and preparation method thereof |
| CN105624095A (en) * | 2014-10-28 | 2016-06-01 | 中国石油化工股份有限公司 | Enrichment culture method for nitrifying bacteria by waste water generated in catalyst production process |
| CN104548921A (en) * | 2014-12-23 | 2015-04-29 | 北京伊普国际水务有限公司 | Biological deodorization filling material and preparation method thereof |
| CN107603909A (en) * | 2017-09-30 | 2018-01-19 | 河北天泓环保科技有限公司 | Sewage treatment microbial inoculant and its application method |
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Application publication date: 20191231 |