CN100404436C - Culture method of aerobic nitrosated granular sludge - Google Patents
Culture method of aerobic nitrosated granular sludge Download PDFInfo
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- CN100404436C CN100404436C CNB2005100118574A CN200510011857A CN100404436C CN 100404436 C CN100404436 C CN 100404436C CN B2005100118574 A CNB2005100118574 A CN B2005100118574A CN 200510011857 A CN200510011857 A CN 200510011857A CN 100404436 C CN100404436 C CN 100404436C
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- 239000010802 sludge Substances 0.000 title claims abstract description 52
- 238000012136 culture method Methods 0.000 title 1
- 239000002351 wastewater Substances 0.000 claims abstract description 36
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 10
- 239000001301 oxygen Substances 0.000 claims abstract description 10
- 238000005273 aeration Methods 0.000 claims abstract description 9
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 8
- 239000011573 trace mineral Substances 0.000 claims abstract description 8
- 238000004088 simulation Methods 0.000 claims description 11
- 239000008187 granular material Substances 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 3
- 150000003016 phosphoric acids Chemical class 0.000 claims description 3
- 235000012538 ammonium bicarbonate Nutrition 0.000 claims description 2
- 150000003863 ammonium salts Chemical class 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017550 sodium carbonate Nutrition 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 238000004065 wastewater treatment Methods 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract 2
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000010796 biological waste Substances 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 9
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 8
- 230000000694 effects Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 3
- 238000007034 nitrosation reaction Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000010008 shearing Methods 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000009280 upflow anaerobic sludge blanket technology Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- QGZKDVFQNNGYKY-OUBTZVSYSA-N Ammonia-15N Chemical compound [15NH3] QGZKDVFQNNGYKY-OUBTZVSYSA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- 241001495402 Nitrococcus Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000003450 growing effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- -1 pH value 7.2~7.8 Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Activated Sludge Processes (AREA)
Abstract
The present invention relates to a method for culturing aerobic nitrosifying granular sludge, which belongs to the technical field of biological waste water treatment. In order to solve the problems of low volume load, low removal rate, etc. which are caused by low sludge concentration in the existing nitrosifying reactor, the present invention provides the method for culturing aerobic nitrosifying granular sludge, which comprises the steps that activated sludge is put into an up-flow type aerobic reactor provided with a three phase separator; simulated waste water of which the ammonia nitrogen concentration is from 200 to 800 mg/L is supplied, and a proper amount of phosphate and a proper number of trace elements are also added; after flowing through the sludge, the waste water flows out from the upper part of the reactor, air is adopted for aeration simultaneously, and the air speed is from 20 to 50 L/h; the temperature, the pH value, the dissolved oxygen, the hydraulic detention time, etc. in the reactor are controlled, and after a period of time of stable operation, the sludge is obtained. The nitrosifying granular sludge obtained by adopting the method of the present invention has high performance and can increase the volume load of the reactor, the removal efficiency and the operating stability.
Description
Technical field
The present invention relates to a kind of cultural method of aerobic nitrosated granular sludge, belong to the biological wastewater treatment technology field.
Background technology
Along with the aggravation of nitrate pollution and the enhancing of people's environmental protection consciousness, the wastewater biological denitrificaion technology has caused the common concern of countries in the world, becomes the important research direction of water pollution control.The nitrite type bio-denitrification technology has been subjected to people's common concern owing to have advantages such as cutting down the consumption of energy, save carbon source, minimizing mud growing amount.The core of nitrite type bio-denitrification technology is that nitrifying process is controlled at the nitrous acid stage, usually utilize Nitrosomas and the intrinsic difference of Nitromonas dynamics, adopt controlled temperature, dissolved oxygen concentration, pH value, ammonia nitrogen loading and mud age etc. that the microbial life influence factor that two class bacteria growings produce Different Effects is realized existing at present relevant report.But because nitrococcus is poky autotrophic bacteria, and interrelated between each controlling factor in the operational conditions, it is lower to make in the nitrosation reaction device of present successful operation that ubiquity load, and clearance is on the low side, problems such as fluctuation of service.Granule sludge is that a large amount of bacteriums assemble the particulate state microbial aggregate with good settling property that growth forms, and granule sludge is key and the prerequisite that high efficiency anaerobic reactor such as UASB, EGSB and IC etc. realize efficient operation.In the nitrosation reaction device, turn out nitrosated granular sludge, can increase the biomass in the reactor, improve operation stability and treatment efficiency with greater activity.
Summary of the invention
Hang down and problems such as clearance is low in order to solve the reactor volume load that causes owing to sludge concentration is low in the existing nitrosation reaction device, the invention provides a kind of cultural method of aerobic nitrosated granular sludge, step is as follows:
1) chooses the upflowing aerobic reactor that active sludge is packed into and is furnished with triphase separator;
2) preparation does not contain the simulation ammonia nitrogen waste water of organic carbon source, and ammonia nitrogen concentration is 200～800mg/L in the described simulation ammonia nitrogen waste water; In the simulation ammonia nitrogen waste water, also need add the normal growth that an amount of phosphoric acid salt and trace element guarantee microorganism;
3) bottom from above-mentioned reactor pumps into described simulation ammonia nitrogen waste water, waste water is flowed through and is flowed out from reactor top behind the mud, aeration head is equipped with in the bottom of reactor, when flowing through reactor, adopts waste water air aeration, air speed is 20～50L/h, utilizes air-flow to shear generation and the growth that promotes granule sludge;
4) keep the interior temperature of reactor between 30～35 ℃, the pH value is between 6.4～8.5, and dissolved oxygen is between 4～7mg/L, and hydraulic detention time is 5～12 hours, after steady running for some time, obtains nitrosated granular sludge in reactor.
The present invention utilizes air-flow to shear in the upflowing aerobic reactor and directly turns out the nitrosated granular sludge with superperformance, can make reactor volumetric loading, remove efficient and aspect such as stable all is greatly improved.
Embodiment
Hereinafter will further specify the present invention.
At first active sludge is packed in the upflowing aerobic reactor of being furnished with triphase separator into the effect of the triphase separator cotton-shaped mud in the seed sludge that is to dam.Because the nitrite bacteria growth scope is wider, so available mud has in anaerobic activated sludge, anaerobic grain sludge and the aerobic activated sludge one or more.
The simulated wastewater that employing contains ammonia nitrogen starts reactor, and ammonia nitrogen concentration is 200～800mg/L in the waste water, and the ammonium salt in the described simulation ammonia nitrogen waste water is one or both in volatile salt and the bicarbonate of ammonia.An amount of phosphoric acid salt and trace element in simulated wastewater, have also been added to guarantee the normal growth of microorganism, the concentration of phosphoric is 0.01～0.1g/L, trace element concentration such as Zn, Co, Mn, Cu, Mo, Ca and Fe are 0.0005～0.005g/L, do not contain organic carbon source in the waste water.The ratio of the inorganic carbon in the simulated wastewater, nitrogen and phosphorus meets the nitrite bacteria required scope of growing, and trace element is the required somatomedin of nitrite bacteria.
Pump into described simulated wastewater from the bottom of described reactor, waste water is flowed through and is flowed out from reactor top behind the mud, and aeration head is equipped with in the bottom of reactor, adopts air aeration when waste water is flowed through reactor, and air speed is 20～50L/h.When reactive force and particle sludge intensity adapt in the granule sludge forming process, granule sludge is produced smoothly and kept, the present invention produces upstream in the air aeration process, the mutual blending of three in upstream and mud and the process that waste water contacts, form shearing force, because the gas-water ratio of waste water is very big in the blending process, so promote among the present invention that the condition of particle generation mainly is the gas shearing force, utilizing the gas shearing force to form granule sludge is characteristics of the present invention.Thereby the too low meeting of air speed causes the mass-transfer efficiency of the low reduction of mud bed degree of mixing oxygen, and the processing efficiency of reactor is low, and the too high meeting of air speed causes the granule sludge fragmentation.
For guaranteeing the abundant growth of nitrite bacteria, operating parameter to following reactor is controlled: 30～35 ℃ of temperature, pH value 6.4～8.5, the activity of nitrite bacteria is lower outside this scope, be unfavorable for the steady running of reactor, the pH value can be regulated by add alkaline matter in described simulated wastewater, and described alkaline matter is one or more in yellow soda ash, sodium bicarbonate and the sodium hydroxide; Dissolved oxygen 4～7mg/L, the activity of nitrite bacteria is lower under the low dissolved axygen condition, and the processing efficiency of reactor is low; High-solubility oxygen can promote the growth of nitrobacteria, and reactor can not rest on the nitrosification stage; Hydraulic detention time 5～12 hours, the too short sludge loss of hydraulic detention time is serious, the reactor fluctuation of service, hydraulic detention time is oversize, and the processing efficiency of reactor is too low; After steady running for some time, just can obtain nitrosated granular sludge in the reactor.
Further describe the present invention below by several specific exampless.
Embodiment 1
Even the aerobic activated sludge in certain nitrator in anaerobic grain sludge in certain UASB reactor of brewery wastewater treatment and the laboratory by 10: 1 mixed.Then mixed soil is inoculated in the upflowing aerobic reactor, the cumulative volume of this reactor is 3.05L, and wherein the reaction zone volume is 1.60L, and settling region 1.04L is furnished with triphase separator in the settling region, and the sludge concentration in the inoculation post-reactor is 2.34gSS/L.Ammonia nitrogen concentration is 200～600mg/L in the simulated wastewater, has also added KH in the above-mentioned waste water
2PO
4And trace element, concentration is respectively 0.05gP/L, 0.001gZn/L, 0.0005gCo/L, 0.002gMn/L, 0.0008gCu/L, 0.0012gMo/L, 0.003gCa/L and 0.002gFe/L, does not contain organic carbon source in the simulated wastewater.The operational condition of reactor is: 30～35 ℃ of temperature, and pH value 6.4～7.7, hydraulic detention time 8～12 hours adopts air aeration, and air speed is 20～30L/h, and the dissolved oxygen in the reactor is controlled at 4～6mg/L.Steady running was observed brown nitrosated granular sludge in the post-reactor in 96 days.The main particle size range of granule sludge is 0.8～1.4mm, wherein accounts for 40% greater than 1.0mm; The settling velocity average out to 38m/h of granule sludge.
The nitrosated granular sludge of turning out is put into another upflowing aerobic reactor treatment of simulated waste water, at ammonia nitrogen volumetric loading 2.5kg/ (m
3D) under the condition, ammonia-N removal rate all can reach more than 95%, and product Central Asia nitrate nitrogen accounts for more than 97% of total nitric nitrogen, and this reactor serviceability is good, and has advantages of higher stability.
Embodiment 2
Adopt the seed sludge identical, pH value 7.5～8.5, hydraulic detention time 5～8 hours with embodiment 1, air speed is 30～50L/h, dissolved oxygen is controlled at 6～7mg/L, and all the other operating process are identical with embodiment 1, and cultured continuously nitrosated granular sludge occurred in the post-reactor of the left and right sides in 75 days.
Embodiment 3
Adopt the seed sludge identical with embodiment 1, Ca, Fe concentration in the change trace element, 0.005gCa/L and 0.001gFe/L, all the other operating process are identical with embodiment 1, and cultured continuously nitrosated granular sludge occurred in the post-reactor of the left and right sides in 124 days.
Embodiment 4
Adopt the anaerobic grain sludge in certain EGSB reactor of handling starch wastewater, ammonia nitrogen concentration is 200～300mg/L in the simulated wastewater, and all the other operating process are identical with embodiment 1, and cultured continuously nitrosated granular sludge occurred in the post-reactor of the left and right sides in 156 days.
Embodiment 5
Adopt the aerobic activated sludge in certain nitrator in the laboratory, ammonia nitrogen concentration is 600～800mg/L in the simulated wastewater, pH value 7.2～7.8, air speed is 40～50L/h, dissolved oxygen is controlled at 6～7mg/L, all the other operating process are identical with embodiment 1, and cultured continuously nitrosated granular sludge occurred in the post-reactor of the left and right sides in 115 days.
Claims (3)
1. the cultural method of an aerobic nitrosated granular sludge is characterized in that, described method comprises the steps:
1) chooses the upflowing aerobic reactor that active sludge is packed into and is furnished with triphase separator;
2) preparation does not contain the simulation ammonia nitrogen waste water of organic carbon source, and ammonia nitrogen concentration is 200～800mg/L in the described simulation ammonia nitrogen waste water; In the simulation ammonia nitrogen waste water, also need add the normal growth that an amount of phosphoric acid salt and trace element guarantee microorganism;
3) bottom from above-mentioned reactor pumps into described simulation ammonia nitrogen waste water, waste water is flowed through and is flowed out from reactor top behind the mud, aeration head is equipped with in the bottom of reactor, when flowing through reactor, adopts waste water air aeration, air speed is 20～50L/h, utilizes air-flow to shear generation and the growth that promotes granule sludge;
4) keep the interior temperature of reactor between 30～35 ℃, the pH value is between 6.4～8.5, and dissolved oxygen is between 4～7mg/L, and hydraulic detention time is 5～12 hours, after steady running for some time, obtains nitrosated granular sludge in reactor.
2. the cultural method of a kind of aerobic nitrosated granular sludge according to claim 1, it is characterized in that: the ammonium salt in the described simulation ammonia nitrogen waste water is one or both in volatile salt and the bicarbonate of ammonia.
3. the cultural method of a kind of aerobic nitrosated granular sludge according to claim 1, it is characterized in that: the pH value in the described reactor is regulated by add alkaline matter in described simulation ammonia nitrogen waste water, and described alkaline matter is one or more in yellow soda ash, sodium bicarbonate and the sodium hydroxide.
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| CNB2005100118574A CN100404436C (en) | 2005-06-03 | 2005-06-03 | Culture method of aerobic nitrosated granular sludge |
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| CNB2005100118574A CN100404436C (en) | 2005-06-03 | 2005-06-03 | Culture method of aerobic nitrosated granular sludge |
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| CN1699217A CN1699217A (en) | 2005-11-23 |
| CN100404436C true CN100404436C (en) | 2008-07-23 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100381373C (en) * | 2006-03-09 | 2008-04-16 | 南京大学 | Method for cultivating nitrified granular sludge using anaerobic granular sludge |
| CN100393869C (en) * | 2006-10-20 | 2008-06-11 | 山东大学 | Use of rare-earth element in cultivating aerobic particle mud and controlling mud expansion |
| CN101928067B (en) * | 2009-06-19 | 2012-05-30 | 东莞理工学院 | Culture method of aerobic denitrification particle sludge |
| CN103121750B (en) * | 2013-01-08 | 2014-02-19 | 北京工业大学 | High-efficiency culture method of urban domestic sewage nitrosation granule sludge |
| CN103601287B (en) * | 2013-11-17 | 2016-03-09 | 北京工业大学 | A kind of cultural method of aerobic nitrosated granular sludge |
| CN103663680B (en) * | 2013-11-25 | 2015-01-07 | 北京工业大学 | Method for cultivating aerobic half-nitrosation granular sludge in continuous flow manner |
| CN103708609B (en) * | 2013-12-15 | 2015-05-20 | 北京工业大学 | Quick culture method of nitrosation granular sludge |
| CN103922466B (en) * | 2014-03-29 | 2015-09-02 | 北京工业大学 | A kind of nitrosated granular sludge cultural method of normal temperature process city domestic sewage |
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| CN1699217A (en) | 2005-11-23 |
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