CN104711213A - Accelerant facilitating growth of salt-resistive nitrifying bacteria in high-salt wastewater - Google Patents
Accelerant facilitating growth of salt-resistive nitrifying bacteria in high-salt wastewater Download PDFInfo
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- CN104711213A CN104711213A CN201510110266.6A CN201510110266A CN104711213A CN 104711213 A CN104711213 A CN 104711213A CN 201510110266 A CN201510110266 A CN 201510110266A CN 104711213 A CN104711213 A CN 104711213A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
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Abstract
The invention discloses an accelerant facilitating the growth of salt-resistive nitrifying bacteria in high-salt wastewater. The accelerant comprises 30-40g sodium chloride, 0.2-0.6g ammonium sulfate, 0.01-0.05g magnesium sulfate heptahydrate, 0.8-1.5g dipotassium phosphate, 0.01-0.05g ferrous sulfate, 7-8.2g calcium chloride, 1-1.7g sodium bicarbonate, 0-0.5g sodium nitrite, 1-5ml vitamin liquid, 1-5ml trace element solution, and 5-10ml bacterium solution per 1000ml water, wherein pH (potential of hydrogen) is 7.8. The accelerant facilitating the growth of the salt-resistive nitrifying bacteria in the high-salt wastewater is free of organic matters such as sugar, contains the inoculated bacterium solution, is suitable for being directly put into the high-salt wastewater for use in an ammonia nitrogen removal technology by a biological method, and allows the high-salt wastewater to be capable of quickly having an environment appropriate for the growth of the salt-resistive nitrifying bacteria. Under the condition that other conditions are the same, after the accelerant is used, a removal rate of ammonia nitrogen can be increased by above 25%, and the accelerant meets requirements on removal of ammonia nitrogen from the high-salt wastewater by a biochemical method.
Description
Technical field
The present invention relates to waste water microbiological treatment field, be specifically related to a kind of promotor of removing the salt tolerant nitrobacteria growth of ammonia nitrogen in high-salt wastewater for biological process.
Background technology
In water body, ammonia-nitrogen content is too high is the one of the main reasons causing the body eutrophication such as river, lake.Be exactly biological process for the most economical conventional method of the removal of ammonia nitrogen in water body.It is nitrite etc. that biological process removal ammonia nitrogen mainly carries out nitrification by mineralized nitrogen by nitrifier under aerobic condition.Nitrifier mostly is chemosynthetic autotroph, and generation time is long, growth velocity is slow, and simultaneously responsive for salinity, this makes the very difficult biological process of the ammonia nitrogen in high-salt wastewater remove.Find through retrieval, application number disclosed in prior art is the Chinese invention patent of 200510111876.4, discloses a kind of nitrobacteria culture promoter, uses this promotor can improve ammonia nitrogen removal frank under other identical conditions and reaches more than 20%.Application number is the Chinese invention patent of 2011103245401, discloses a kind of liquid nutrient medium promoting salt tolerant nitrobacteria growth, can promote the growth in the medium of salt tolerant nitrifier.But can directly be invested in high-salt wastewater disclosed in also not having, be beneficial to the promotor of salt tolerant nitrobacteria growth.Therefore, the research and development being beneficial to the promotor of salt tolerant nitrobacteria growth in high-salt wastewater seem particularly important.
Summary of the invention
The object of this invention is to provide a kind of promotor of salt tolerant nitrobacteria growth, it can directly drop in high-salt wastewater, coordinate conditions such as regulating DO and pH in sewage can make salt tolerant nitrifier growth and breeding at short notice in water body, thus when solving ammonia nitrogen in biological process removal high-salt wastewater, salt tolerant nitrobacteria growth is this technical problem slowly.
Object of the present invention is achieved through the following technical solutions:
Be beneficial to a promotor for salt tolerant nitrobacteria growth in high-salt wastewater, it is characterized in that: the composition of this promotor comprises: containing sodium-chlor 30-40g, ammonium sulfate 0.2-0.6g, bitter salt 0.01-0.05g, dipotassium hydrogen phosphate 0.8-1.5g, ferrous sulfate 0.01-0.05g calcium chloride 7-8.2g, sodium bicarbonate 1-1.7g, Sodium Nitrite 0-0.5g, VITAMIN liquid 1-5ml, trace element solution 1-5ml, bacterium liquid 5-10ml, pH=7.8 in every 1000ml water;
Described VITAMIN liquid consists of: containing xitix 0.01-0.02g, riboflavin 0.01-0.02g, cobalami 0.02-0.03g, citric acid 0.02-0.04g, para-amino benzoic acid 0.01-0.02g, pyridoxal hydrochloride 0.02-0.05g in every 1000ml water;
Described trace element solution consists of: containing zinc sulfate 2.1-2.4g, four chloride hydrate manganese 5-5.1g, Salzburg vitriol 1.5-1.7g, cobalt chloride hexahydrate 1.4-1.5g, pH=7 in every 1000ml water;
Described bacterium liquid consists of: inoculate 10% nitrifier bacterial classification in every 1000ml water.
For realizing the object of the invention further, preferably containing sodium-chlor 30-35g, ammonium sulfate 0.3-0.5g, bitter salt 0.01-0.03g, dipotassium hydrogen phosphate 1-1.5g, ferrous sulfate 0.01-0.03g, calcium chloride 7.5-8.2g, sodium bicarbonate 1.2-1.7g, Sodium Nitrite 0-0.2g, VITAMIN liquid 1-3ml, trace element solution 1-3ml, bacterium liquid 6-8ml, pH=7.8 in every 1000ml water.
For further realizing the object of the invention, further preferably containing sodium-chlor 30g, ammonium sulfate 0.5g, bitter salt 0.03g, dipotassium hydrogen phosphate 1.4g, ferrous sulfate 0.03g calcium chloride 7.5g, sodium bicarbonate 1.6g, VITAMIN liquid 3ml, trace element solution 3ml, bacterium liquid 7ml, pH=7.8 in every 1000ml water.
Compared with prior art, a kind of advantage being beneficial to the promotor of salt tolerant nitrobacteria growth in high-salt wastewater of the present invention mainly comprises four aspects: one is that this promotor is not containing organism such as sugars, COD content in waste water can not be increased, add the nitrifier nutrient solution of sugars etc. compared to other more economically simultaneously, and the sodium bicarbonate that it contains, dipotassium hydrogen phosphate etc. can play shock absorption, regulate pH in waste water, make nitrifier can under most suitable pH quick growth and breeding; Two is the bacterium liquid that this promotor contains inoculation, along with promotor is invested in after in high-salt wastewater, can conform very soon and Fast-propagation growth; Three is that this promotor is adapted at directly throwing in high-salt wastewater in biological process removal ammonia nitrogen technique using, make high-salt wastewater can have the environment of suitable salt tolerant nitrobacteria growth fast, the problem of salt tolerant nitrobacteria growth difficulty when solving Biochemical method high-salt wastewater.Four is when other conditions are identical, and after employing this promotor, the clearance of ammonia nitrogen can improve more than 25%, well meets the requirement that biochemical process removes ammonia nitrogen in high-salt wastewater.
Embodiment
Below in conjunction with embodiment, the present invention is further detailed.
Embodiment one:
Promotor of the present invention added in certain marine oil chemical industry high-salt wastewater, actual conditions is as follows: influent quality is that COD=357mg/l, NH3-N=98.5mg/l, saltiness are about 3%, between pH value 7.5-8.5.Adopt SBR technique to process, a cycle of operation is 13-24h, and in each cycle of operation, water inlet 30min, aeration 10-20h, precipitation 1-2h, draining 1h, remaining time is lay-up period.Temperature is 30 DEG C.Adding the ratio of promotor of the present invention in waste water is 10ml/l (control group is not for adding this promotor), the composition of this promotor comprises: containing sodium-chlor 40g in every 1000ml water, ammonium sulfate 0.2g, bitter salt 0.01g, dipotassium hydrogen phosphate 0.8g, ferrous sulfate 0.01g calcium chloride 7g, sodium bicarbonate 1g, (VITAMIN liquid consists of VITAMIN liquid 2ml: containing xitix 0.01g in every 1000ml water, riboflavin 0.01g, cobalami 0.02g, citric acid 0.02g, para-amino benzoic acid 0.01g, pyridoxal hydrochloride 0.02g), (trace element solution consists of trace element solution 2ml: containing zinc sulfate 2.1g in every 1000ml water, four chloride hydrate manganese 5.1g, Salzburg vitriol 1.5g, cobalt chloride hexahydrate 1.4g, pH=7), bacterium liquid 6ml, pH=7.8.
After a cycle of operation 13h (in the cycle of operation, water inlet 30min, aeration 10h, precipitation 1h, draining 1h, remaining time is lay-up period), the results are shown in Table 1:
Table 1 processes effluent quality
| Project | Water outlet | Clearance | Control group water outlet | Control group clearance |
| NH3-N(mg/l) | 19.7 | 80％ | 45.1 | 54.2％ |
| COD(mg/l) | 102 | 71.4％ | 118 | 66.9％ |
Embodiment two:
Promotor of the present invention added in certain marine oil chemical industry high-salt wastewater, actual conditions is as follows: influent quality is that COD=369mg/l, NH3-N=97.8mg/l, saltiness are about 3%, between pH value 7.5-8.5.Adopt SBR technique to process, a cycle of operation is 13-24h, and in each cycle of operation, water inlet 30min, aeration 10-20h, precipitation 1-2h, draining 1h, remaining time is lay-up period.Temperature is 30 DEG C.Adding the ratio of promotor of the present invention in waste water is 20ml/l (control group is not for adding this promotor), the composition of this promotor comprises: containing sodium-chlor 35g in every 1000ml water, ammonium sulfate 0.5g, bitter salt 0.03g, dipotassium hydrogen phosphate 1.2g, ferrous sulfate 0.05g calcium chloride 7.5g, sodium bicarbonate 1.5g, Sodium Nitrite 0.5g, (VITAMIN liquid consists of VITAMIN liquid 3ml: containing xitix 0.01g in every 1000ml water, riboflavin 0.01g, cobalami 0.03g, citric acid 0.04g, para-amino benzoic acid 0.02g, pyridoxal hydrochloride 0.03g), (trace element solution consists of trace element solution 3ml: containing zinc sulfate 2.2g in every 1000ml water, four chloride hydrate manganese 5g, Salzburg vitriol 1.6g, cobalt chloride hexahydrate 1.5g, pH=7), bacterium liquid 10ml, pH=7.8.
After a cycle of operation 18h (in the cycle of operation, water inlet 30min, aeration 15h, precipitation 1h, draining 1h, remaining time is lay-up period), the results are shown in Table 2:
Table 2 processes effluent quality
| Project | Water outlet | Clearance | Control group water outlet | Control group clearance |
| NH3-N(mg/l) | 10.1 | 89.7％ | 42.6 | 56.4％ |
| COD(mg/l) | 110 | 70.2％ | 115 | 68.8％ |
Embodiment three:
Promotor of the present invention added in certain marine oil chemical industry high-salt wastewater, actual conditions is as follows: influent quality is that COD=347mg/l, NH3-N=98.2mg/l, saltiness are about 3%, between pH value 7.5-8.5.Adopt SBR technique to process, a cycle of operation is 13-24h, and in each cycle of operation, water inlet 30min, aeration 10-20h, precipitation 1-2h, draining 1h, remaining time is lay-up period.Temperature is 30 DEG C.Adding the ratio of promotor of the present invention in waste water is 30ml/l (control group is not for adding this promotor), the composition of this promotor comprises: containing sodium-chlor 30g in every 1000ml water, ammonium sulfate 0.5g, bitter salt 0.03g, dipotassium hydrogen phosphate 1.4g, ferrous sulfate 0.03g calcium chloride 7.5g, sodium bicarbonate 1.6g, (VITAMIN liquid consists of VITAMIN liquid 3ml: containing xitix 0.02g in every 1000ml water, riboflavin 0.01-0.02g, cobalami 0.03g, citric acid 0.04g, para-amino benzoic acid 0.02g, pyridoxal hydrochloride 0.05g), (trace element solution consists of trace element solution 3ml: containing zinc sulfate 2.4g in every 1000ml water, four chloride hydrate manganese 5.1g, Salzburg vitriol 11.7g, cobalt chloride hexahydrate 1.5g, pH=7), bacterium liquid 7ml, pH=7.8.
After a cycle of operation 24h (in the cycle of operation, water inlet 30min, aeration 20h, precipitation 2h, draining 1h, remaining time is lay-up period), the results are shown in Table 2:
Table 2 processes effluent quality
| Project | Water outlet | Clearance | Control group water outlet | Control group clearance |
| NH3-N(mg/l) | 5.5 | 94.4％ | 48.2 | 50.9％ |
| COD(mg/l) | 98 | 71.8％ | 103 | 70.3％ |
Claims (3)
1. utilize a promotor for salt tolerant nitrobacteria growth in high-salt wastewater, it is characterized in that: the composition of described promotor comprises: containing sodium-chlor 30-40g, ammonium sulfate 0.2-0.6g, bitter salt 0.01-0.05g, dipotassium hydrogen phosphate 0.8-1.5g, ferrous sulfate 0.01-0.05g, calcium chloride 7-8.2g, sodium bicarbonate 1-1.7g, Sodium Nitrite 0-0.5g, VITAMIN liquid 1-5ml, trace element solution 1-5ml, bacterium liquid 5-10ml, pH=7.8 in every 1000ml water;
Wherein said VITAMIN liquid consists of: containing xitix 0.01-0.02g, riboflavin 0.01-0.02g, cobalami 0.02-0.03g, citric acid 0.02-0.04g, para-amino benzoic acid 0.01-0.02g, pyridoxal hydrochloride 0.02-0.05g in every 1000ml water;
Described trace element solution consists of: containing zinc sulfate 2.1-2.4g, four chloride hydrate manganese 5-5.1g, Salzburg vitriol 1.5-1.7g, cobalt chloride hexahydrate 1.4-1.5g, pH=7 in every 1000ml water;
Described bacterium liquid consists of: inoculate 10% nitrifier bacterial classification in every 1000ml water.
2. promotor according to claim 1, is characterized in that: containing sodium-chlor 30-35g, ammonium sulfate 0.3-0.5g, bitter salt 0.01-0.03g, dipotassium hydrogen phosphate 1-1.5g, ferrous sulfate 0.01-0.03g, calcium chloride 7.5-8.2g, sodium bicarbonate 1.2-1.7g, Sodium Nitrite 0-0.2g, VITAMIN liquid 1-3ml, trace element solution 1-3ml, bacterium liquid 6-8ml, pH=7.8 in every 1000ml water.
3. promotor according to claim 1, is characterized in that: containing sodium-chlor 30g, ammonium sulfate 0.5g, bitter salt 0.03g, dipotassium hydrogen phosphate 1.4g, ferrous sulfate 0.03g, calcium chloride 7.5g, sodium bicarbonate 1.6g, VITAMIN liquid 3ml, trace element solution 3ml, bacterium liquid 7ml, pH=7.8 in every 1000ml water.
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Cited By (11)
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|---|---|---|---|---|
| CN105152357A (en) * | 2015-09-05 | 2015-12-16 | 王磊 | Medical intermediate biological waste water treatment accelerant and preparing method and application thereof |
| CN105174492A (en) * | 2015-10-09 | 2015-12-23 | 天津大学 | Accelerant for quick recovery of nitrifying bacteria after suppression by heavy metals |
| CN106745697A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of method for controlling biochemical processing procedure of sewage nitration reaction process |
| CN107058184A (en) * | 2017-05-19 | 2017-08-18 | 北京易水环境技术服务有限公司 | Basic nutrient solution and its preparation method and application |
| CN107663511A (en) * | 2017-11-30 | 2018-02-06 | 沈国青 | A kind of culture medium of nitrite bacteria and preparation method thereof |
| CN108103000A (en) * | 2017-12-27 | 2018-06-01 | 齐鲁工业大学 | A kind of growth promoter for improving anaerobic ammonia oxidizing bacteria and preparation method thereof |
| CN108754160A (en) * | 2018-07-26 | 2018-11-06 | 江苏理工学院 | A kind of recoverying and utilizing method of pyridoxal Mn-bearing waste water |
| CN110510752A (en) * | 2019-08-01 | 2019-11-29 | 山东昱泰环保工程有限公司 | A kind of microorganism growth-promoting synergy nutritional agents and its preparation method and application |
| CN110980961A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Denitrifying microbial inoculum and sewage treatment technology applying same |
| CN111099722A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for promoting denitrification and application thereof |
| CN111099743A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for enhancing denitrification and denitrification of wastewater and denitrification method thereof |
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| CN105152357B (en) * | 2015-09-05 | 2017-06-06 | 泉州东行贸易有限公司 | A kind of pharmaceutical intermediate wastewater accelerant for biological treatment and preparation method and application |
| CN105152357A (en) * | 2015-09-05 | 2015-12-16 | 王磊 | Medical intermediate biological waste water treatment accelerant and preparing method and application thereof |
| CN105174492B (en) * | 2015-10-09 | 2019-07-09 | 天津大学 | A kind of nitrifier fast quick-recovery promotor after being inhibited by heavy metal |
| CN105174492A (en) * | 2015-10-09 | 2015-12-23 | 天津大学 | Accelerant for quick recovery of nitrifying bacteria after suppression by heavy metals |
| CN106745697A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of method for controlling biochemical processing procedure of sewage nitration reaction process |
| CN106745697B (en) * | 2015-11-19 | 2020-07-07 | 中国石油化工股份有限公司 | Method for controlling nitration reaction process in sewage biochemical treatment process |
| CN107058184A (en) * | 2017-05-19 | 2017-08-18 | 北京易水环境技术服务有限公司 | Basic nutrient solution and its preparation method and application |
| CN107663511A (en) * | 2017-11-30 | 2018-02-06 | 沈国青 | A kind of culture medium of nitrite bacteria and preparation method thereof |
| CN108103000A (en) * | 2017-12-27 | 2018-06-01 | 齐鲁工业大学 | A kind of growth promoter for improving anaerobic ammonia oxidizing bacteria and preparation method thereof |
| CN108754160A (en) * | 2018-07-26 | 2018-11-06 | 江苏理工学院 | A kind of recoverying and utilizing method of pyridoxal Mn-bearing waste water |
| CN108754160B (en) * | 2018-07-26 | 2020-05-08 | 江苏理工学院 | Method for recycling manganese-containing pyridoxal wastewater |
| CN111099722A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for promoting denitrification and application thereof |
| CN111099743A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for enhancing denitrification and denitrification of wastewater and denitrification method thereof |
| CN111099743B (en) * | 2018-10-26 | 2022-06-07 | 中国石油化工股份有限公司 | Composition for enhancing denitrification and denitrification of wastewater and denitrification method thereof |
| CN110510752A (en) * | 2019-08-01 | 2019-11-29 | 山东昱泰环保工程有限公司 | A kind of microorganism growth-promoting synergy nutritional agents and its preparation method and application |
| CN110510752B (en) * | 2019-08-01 | 2020-04-07 | 山东昱泰环保工程有限公司 | Microbial growth-promoting synergistic nutrient and preparation method and application thereof |
| CN110980961A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Denitrifying microbial inoculum and sewage treatment technology applying same |
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Address after: 100010 Beijing, Chaoyangmen, North Street, No. 25, No. Applicant after: CHINA NATIONAL OFFSHORE OIL Corp. Applicant after: CNOOC TIANJIN CHEMICAL RESEARCH & DESIGN INSTITUTE Co.,Ltd. Applicant after: CNOOC ENERGY TECHNOLOGY & SERVICES Ltd. Address before: 100010 Beijing, Chaoyangmen, North Street, No. 25, No. Applicant before: CHINA NATIONAL OFFSHORE OIL Corp. Applicant before: CNOOC TIANJIN CHEMICAL RESEARCH & DESIGN INSTITUTE Applicant before: CNOOC ENERGY TECHNOLOGY & SERVICES Ltd. |
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Effective date of registration: 20161020 Address after: No. three road 300131 Tianjin city Hongqiao District dingzigu No. 85 Applicant after: CNOOC TIANJIN CHEMICAL RESEARCH & DESIGN INSTITUTE Co.,Ltd. Applicant after: CNOOC ENERGY TECHNOLOGY & SERVICES Ltd. Address before: 100010 Beijing, Chaoyangmen, North Street, No. 25, No. Applicant before: CHINA NATIONAL OFFSHORE OIL Corp. Applicant before: CNOOC TIANJIN CHEMICAL RESEARCH & DESIGN INSTITUTE Co.,Ltd. Applicant before: CNOOC ENERGY TECHNOLOGY & SERVICES Ltd. |
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