CN102319547A - High-precision and low-speed stirring system with low heat quantity - Google Patents
High-precision and low-speed stirring system with low heat quantity Download PDFInfo
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- CN102319547A CN102319547A CN2011101830190A CN201110183019A CN102319547A CN 102319547 A CN102319547 A CN 102319547A CN 2011101830190 A CN2011101830190 A CN 2011101830190A CN 201110183019 A CN201110183019 A CN 201110183019A CN 102319547 A CN102319547 A CN 102319547A
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Abstract
The invention discloses a high-precision and low-speed stirring system with low heat quantity, comprising a stirring device that comprises an upper cover, a lower cover, an electric motor and a stirring linkage device. The upper cover and the lower cover of the stirring device are connected mutually to form a housing of the stirring device; and a stirring bar is connected with the electric motor through the stirring linkage device. The stirring system further comprises a flow guide fan and a partition plate arranged between the upper cover and the lower cover of the stirring device; the stirring linkage device is arranged between the partition plate and the upper cover of the stirring device; the partition plate and the upper cover of the stirring device form an airflow layer; the wind power of the flow guide fan forms an airflow layer which forms convection with environmental air outside the device. By adopting the structure combining double boards with air guidance, heat generated by the electric motor is taken away through the forced convection exchange way of the flow guide fan and the environmental air, so that the temperature of the upper cover of the stirring device is substantially as same as the temperature of the environment, the bottom of a reaction device is heated substantially as same as other parts, and the reaction process is guaranteed to be performed in a controllable temperature state.
Description
Technical field
The present invention relates to a kind of experiment apparatus of electromechanical integration, relate in particular to a kind of high accuracy stirring at low speed system low in calories.
Background technology
In chemistry and Bioexperiment process, often can use agitator, agitator comprises stirrer, the motor in stirred vessel, the stirred vessel, motor provides kinetic energy to stirrer, so that it stirs the liquid in the stirred vessel in stirred vessel.What often use at present is magnetic stirring apparatus.Magnetic stirring apparatus is to produce rotating excitation field by micro motor for driving magnet to drive the stirrer rotation in the container; To reach solution is stirred; Make solution obtain the good heat transfer mass transfer condition; In the temperature of setting, obtain uniformly sufficient hybrid reaction, so can be widely used in fields such as biology, medicine, chemistry, chemical industry.
In the use of small-scale reaction unit (like zooblast reactor etc.), when course of reaction is when reaction unit is placed on isoperibol condition (in incubator) and carries out, the temperature of course of reaction is controlled by environment temperature.And environment temperature is by controlling like incubator.But; Because the heat that motor produced of rotating excitation field is provided; Cause the bottom temp of the reaction unit that is positioned at its top higher than environment temperature; Thereby make the temperature of reactant liquor exceed the several years than environment temperature, this temperature can make follow-up measurement or experimental result inaccurate, can not satisfy in the course of reaction requirement to temperature thus.
In addition, the common range of speeds of magnetic agitation is up to about 2000PRM now, the control accuracy of its rotating speed under low speed (5-250PRM) service condition, and the reappearance precision is all undesirable.
Summary of the invention
The object of the present invention is to provide a kind of high accuracy stirring at low speed system low in calories, have bigger temperature difference, influence the technical problem of measurement result thus with temperature and the device peripheral environment temperature that solves agitating device surface in the prior art.
A kind of high accuracy stirring at low speed system low in calories; Comprise agitating device; Said agitating device comprises agitating device loam cake, agitating device lower cover, motor, stirred vessel, stirrer and stirring linkage, and said agitating device loam cake and agitating device lower cover interconnect forms the agitating device shell, and stirrer is connected with motor through stirring linkage; Also comprise dividing plate and diversion fan; Said dividing plate is arranged between agitating device loam cake and the agitating device lower cover, stirs linkage and is arranged between said dividing plate and the said agitating device loam cake, and said motor is arranged between said dividing plate and the said agitating device lower cover; Said dividing plate and said agitating device loam cake form an airflow layer, and the wind-force of said diversion fan forms airflow layer and device external environment cross-ventilation.
Preferably, through hole on is set on the said dividing plate, covers under the said agitating device lower through-hole is set, said diversion fan is arranged on said going up between through hole and lower through-hole.
Preferably, stir linkage and comprise at least one magnet steel frame, said magnet steel frame is connected with said motor, and said stirrer passes through the magnetic force coupled drive with the stirring linkage.
Preferably, those motors and magnet steel frame be distributed in respectively diversion fan around, said diversion fan is arranged between dividing plate and the agitating device lower cover.
Preferably; Also comprise the stirring control device; Said stirring control device comprises power supply unit, control module, interactive interface unit and I/O unit, and said power supply unit, interactive interface unit and said I/O unit are connected with said control module respectively; Also comprise some electric-motor drive units, said some electric-motor drive units are connected with said control module respectively, and said electric-motor drive unit links to each other with motor.
Preferably, said I/O unit comprises a text display, and said text display is connected through the Serial Port Line that intersects with said control module.
Preferably, said control module comprises controller, and said controller sends pulse to electric-motor drive unit.
Preferably, said electric-motor drive unit is a stepper motor driver, and the motor that is connected with said stepper motor driver is a stepper motor.
Preferably, controller is through sending pulse to stepper motor driver, the angle of the corresponding stepper motor operation of the number of pulse, the speed of the corresponding motor operation of the frequency of pulse.
Owing to adopted above technical scheme, make the present invention compare prior art, have following advantage and good effect:
This device adopts doubling course to combine the structure of air conducting; Carry out the mode with environmental gas forced convertion exchange through diversion fan; The heat that motor produces is taken away; Make the bottom of the reaction unit on this device be heated with other part be influenced by heat basic identical, thereby guaranteed that course of reaction carries out under the state of a Controllable Temperature.
Digital type pulse signal and stepper motor special purpose driver that this device adopts the high accuracy stepper motor and combines controller to send can realize the accurate control of setting.And when comprising 2 covers and above stirring motor in a whole set of agitating device, the mutual same ability of speed discrepancy of stirring motor is precisely controlled.
Description of drawings
Fig. 1 is a kind of structural representation of agitating device of high accuracy stirring at low speed system low in calories;
Fig. 2 is a kind of structure explosion figure of agitating device of high accuracy stirring at low speed system low in calories;
Fig. 3 is a kind of electrical principle structured flowchart of stirring control device of high accuracy stirring at low speed system low in calories.
The specific embodiment
Below in conjunction with accompanying drawing, bright specifically the present invention.
In the use of small-scale reaction unit (like zooblast reactor etc.); When course of reaction is when reaction unit is placed on isoperibol condition (in incubator) and carries out; The temperature of course of reaction is controlled by environment temperature, and environment temperature is by controlling like incubator.Following in the industry standard of animal cell culture device " JB/T 20137-2011 mechanical agitation type animal cell culture jar " to the performance requirement of zooblast reactor:
5.4.5 the temperature of nutrient solution should be able to be set in 37 ℃ and following room temperature, the allowable error scope is ± 0.2 ℃.
5.4.6 the pH value of nutrient solution should be able to be set in 6～9 scopes, the allowable error scope is ± 0.02.
5.4.7 the dissolved oxygen of nutrient solution (DO) should be able to be set in (0～100) % air saturation scope, the allowable error scope is ± 10%.
5.4.8 speed of agitator should be able to be set in (0～250) r/min scope, the allowable error scope is ± 2%.
But; Because the heat that motor produced of rotating excitation field is provided; Cause the bottom temp of the reaction unit that is positioned at its top higher than environment temperature; Thereby make the temperature of reactant liquor exceed the several years than environment temperature, this temperature can make follow-up measurement or experimental result inaccurate, can not satisfy in the course of reaction requirement to temperature thus.The present invention provides a kind of high accuracy stirring at low speed system low in calories; Make the temperature on agitating device surface reduce with the temperature difference of installing the peripheral environment temperature; Satisfied in " JB/T 20137-2011 mechanical agitation type animal cell culture jar " performance requirement, to solve the inaccurate technical problem of measurement result to the zooblast reactor.
Stirring system provided by the invention when stirring with soluble chemistry reagent fast, can be the magnetic agitation mode, also can be non-magnetic agitation mode, and the present invention does not limit it.But under special circumstances; Enzymatic tissue as being write down in " animal cell culture---basic fundamental guide (the 5th edition) " dissociates (seeing the 12.3.3 joint) or suspension culture (is seen the 13.7.4 joint; 13.7.5 in the time of joint), must use magnetic stirring apparatus, magnetic stirring apparatus that following requirement is arranged:
1. motor can not make the culture heating, uses the belt drives of local driven in rotation or outer planting motor;
2. speed must be controlled at below the 50rpm;
3. during low speed, but rotor still mixed volume reach the solution of 10L;
4. can be fit to stir multiple culture simultaneously;
5. can independently adjust the position of each agitator;
6. the rotating speed of each agitator can independent displaying.
In order to satisfy requirement,, specify below in conjunction with accompanying drawing to magnetic stirring apparatus.
See also Fig. 1, Fig. 2 and Fig. 3, stirring system comprises agitating device and stirs control device that agitating device comprises the agitating device shell, stirred vessel, stirrer and motor.Stirred vessel is arranged on the agitating device shell, is provided with in the agitating device shell to stir linkage and motor, and stirrer is arranged in the stirred vessel, and stirrer is connected the liquid that stirs in the stirred vessel through stirring linkage with motor.Stir control device control machine operation, and then the work of control stirrer.In instance, what stir the linkage employing is magnetic means, promptly stir linkage and adopt the magnet steel frame, and stirrer is through magnetic force coupled modes and the interlock of magnetic force frame.
A kind of high accuracy stirring at low speed system low in calories of the present invention, it comprises agitating device, agitating device comprises agitating device loam cake 12, agitating device lower cover 11, magnet steel frame 17 and motor 13.Agitating device loam cake 12 interconnects with agitating device lower cover 11 forms the agitating device shell.Agitating device loam cake 12 comprises upper cover plate and two side panels, and agitating device lower cover 11 comprises lower cover and two other side panel, so that agitating device loam cake 12 can be formed complete agitating device shell with agitating device lower cover 11.This structure is not to be unique, can realize in the prior art that the structure of agitating device shell is very many, and the present invention has repeated no more.
The present invention adopts doubling course to combine the structure of air conducting; Carry out the mode with environmental gas forced convertion exchange through diversion fan; The heat that motor produces is taken away; Make the bottom of the reaction unit on this device be heated with other part be influenced by heat basic identical, thereby guaranteed that course of reaction carries out under the state of a Controllable Temperature.
Stirrer is connected with motor 13 through magnet steel frame 17; Dividing plate 14 is arranged between agitating device loam cake 12 and the agitating device lower cover 11; Magnet steel frame 17 is arranged between dividing plate 14 and the agitating device loam cake 12; Motor 13 is arranged between dividing plate 14 and the agitating device lower cover 11, and dividing plate 14 forms an airflow layer with agitating device loam cake 12, and the wind-force of diversion fan 16 forms airflow layer and device external environment cross-ventilation.
Between agitating device loam cake 12 and the agitating device lower cover 11 dividing plate 14 is set.Dividing plate 14 forms an airflow layer with agitating device loam cake 12.In this device, comprise four magnet steel framves 17 and four motors 13, each magnet steel frame 17 connects a motor 13 respectively, and magnet steel frame 17 is connected with stirrer, and stirrer stirs the liquid in the stirred vessel.Certainly, the number of magnet steel frame 17 and motor 13 is not limited thereto, can carry out concrete setting again according to concrete experimental requirements.This device adopts four usefulness that quantity only illustrates.The middle part of dividing plate 14 is provided with through hole 141 on, and four groups of small through hole 142 are set respectively around the last through hole 141, and each group small through hole 142 is respectively applied for to be placed a magnet steel frame 17 and fix it.The screw that the periphery setting of dividing plate 14 is connected with agitating device lower cover 11, certainly, dividing plate 14 is a variety of with agitating device shell ways of connecting, and this only is a kind of implementation.In this example, those motors 13 and magnet steel frame 17 be distributed in respectively diversion fan 16 around.This set can make that the heat radiation convection effects is good.
In this example; One lower through-hole is set on the agitating device lower cover 11; Diversion fan 16 just runs through between through hole 141 and the lower through-hole, and diversion fan 16 can carry out encapsulation process with dividing plate 14, and same diversion fan 16 carries out encapsulation process with agitating device lower cover 11; Like this, diversion fan 16 can be with in the environment outside the direct strong convection of the heat of airflow layer to the device.
See also table 1, table 1 is a kind of agitating device of high accuracy stirring at low speed system low in calories and the temperature control effect comparison of like product.The temperature-controlled precision of conventional constant incubator, biochemical incubator is generally ± 0.3-0.5 ℃; With respect to environment temperature, the surface temperature rise of agitating device low in calories of the present invention is about 0.2 ℃, in environment temperature-controlled precision scope; Can satisfy requirement of experiment; And the high accuracy stirring at low speed device of the similar purposes of several moneys home and abroad of conduct contrast, its temperature rise with respect to environment temperature reaches 4-8 ℃, and performance low in calories is obviously weaker than the present invention.This temperature difference, for such as thermally sensitive courses of reaction such as animal cell culture, its influence is huge.
The agitating device of table 1 a high accuracy stirring at low speed system low in calories and the temperature control effect comparison of like product
Digital type pulse signal and stepper motor special purpose driver that this device also adopts the high accuracy stepper motor and combines controller to send can realize the accurate control of setting.
Please refer to Fig. 3; Stirring system also comprises the stirring control device; It comprises power supply unit 25, control module 22, interactive interface unit 21 and I/O unit 23, power supply unit 25,22 draws of interactive interface unit, and I/O unit 23 is connected with control module 25 respectively.In the present embodiment, power supply unit 22 can be the power supply that directly can supply power, also can be attaching plug, is connected with power supply to obtain electric energy through this attaching plug; Control module 22 is a controller, and controller can send digital pulse signal; I/O unit 23 is a text display, and text display is connected through the Serial Port Line that intersects with control module 22, employing be the MODBUS rtu protocol.
Stir startup frequency that any No. one motor can be set respectively on the text display of control device, quicken frequency, maximum (top) speed, on text, can show the speed of service of each spindle motor simultaneously, can control the startup of each spindle motor respectively and stop.
On controller, also connect some electric-motor drive units 24; Each electric-motor drive unit 24 connects a motor 13; In the present embodiment; Electric-motor drive unit 24 is a stepper motor driver, and each stepper motor driver also connects a stepper motor, and controller is connected through single line with the stepper motor driver on each road.
Agitating device adopts flat cable to be connected with the stirring control device; Agitating device lower cover 11 sides are provided with an interactive interface 18; Stir on the control device interactive interface module 21 also is set, interactive interface 18, one ends that flat cable one end is positioned on the agitating device are positioned at the interactive interface module 21 that stirs on the control device; At this moment, just accomplish agitating device and stirred the action that control device is connected.In the present embodiment; The about 1-2mm of flat cable thickness; In the time of in agitating device is placed on like the closed environment of incubator etc.,, make things convenient for the door on the incubator etc. to close because the thickness of flat cable is smaller; Help improving the seal of door, reach the service condition (like temperature, gas concentration etc.) that accurately controls environment.
Stepper motor is the open loop control element that electric impulse signal is changed into angular displacement or displacement of the lines.Under the situation of non-overload, the frequency and the umber of pulse of pulse signal are only depended in rotating speed of motor, the position that stops, and do not receive the influence of load variations.
When step actuator receives a pulse signal that is sent by controller, rotate a fixing angle with regard to drive stepping motor by the direction of setting, be called " step angle ".Its rotation is to move step by step with fixing angle.Can come the pilot angle displacement through the control impuls number, thereby reach the purpose of accurate location; Can control speed and the acceleration that motor rotates through the control impuls frequency simultaneously, thereby reach the purpose of speed governing.After adopting the segmentation function, driver can be decomposed into corresponding segmentation number drive stepping motor rotation with each stepping angle.
Segmentation number like driver is N, and the stepping angle of stepper motor is α, the speed of the corresponding motor operation of the frequency of pulse.The segmentation number of the pulse number that controller sends, driver and the relation of the motor shaft anglec of rotation are:
M?=?N×360°/?α°
In the formula:
M ---the pulse number that controller sends,
N ---the segmentation number of driver,
α ---the stepping angle of stepper motor.
Adopt 4 segmentations like stepper motor driver, the step angle of motor is 1.8 degree, and the corresponding pulse number of motor operation one circle is 4 * 360 °/1.8 °=800 so.
This shows that corresponding four subdivision drivers, stepping angle are 1.8 ° stepper motor, controller can reach 1.8 °/360 °/4=0.00125 circle/pulse in theory to the control accuracy of motor
Through the control impuls frequency, can control rotating speed of motor, thereby reach the purpose of speed governing.
For example; Rotating speed as the motor output 250RPM that needs agitating device; Input speed setting value 250RPM on text display at first, controller obtain this instruction back through built-in sequential operation, and the frequency signal of 250 * 800=200000 pulses per minute of output is to stepper motor driver; The rotating speed of drive stepping motor output=200000*0.00125=250RPM drives stirrer and rotates with same rotating speed again.
Based on above-mentioned principle, make the reappearance of speed of agitator also be guaranteed, help the mutual comparison of course of reaction.Likewise, when comprising 2 covers and above stirring motor in a whole set of agitating device, the mutual speed discrepancy of stirring motor equally can be precisely controlled so that experiment compare.
The present invention utilizes the high accuracy characteristic of stepper motor; And combine supporting digital type pulse signal generator and stepper motor special purpose driver, with realize speed of agitator setting value ± 1RPM is with the required precision of the speed difference between interior control, reappearance and the different motor.
In the present invention; Promptly satisfied in the industry standard " JB/T 20137-2011 mechanical agitation type animal cell culture jar " performance requirement to the zooblast reactor; Also satisfied simultaneously take passages in " animal cell culture---basic fundamental guide (the 5th edition) " about requirement to magnetic stirring apparatus; And the slow start and the SECO function of speed of agitator have been increased; Promptly at different cultivation stages mass transfers such as oxygen consumption are required the variation with fingerprinting stress according to zooblast, several time periods are set, each stirs the position can be provided with different rotating speeds in each time period.When stir opening, slowly reach the setting rotating speed, and carry out the transition to the rotating speed of next time period in the time period that sets, solved the timing node deviation of artificial adjustment rotating speed, guaranteed the accurate control and the reappearance of contrast incubation.
More than a disclosed specific embodiment that is merely the application, but the application is not limited thereto, any those skilled in the art can think variation, all should drop in the application's the protection domain.That is to say that 2 technical characterstics of the present invention can satisfy simultaneously, also can be only to satisfy one of them.
Claims (9)
1. high accuracy stirring at low speed system low in calories; Comprise agitating device, said agitating device comprises agitating device loam cake, agitating device lower cover, motor, stirred vessel, stirrer and stirring linkage, and said agitating device loam cake and agitating device lower cover interconnect forms the agitating device shell; Stirrer is connected with motor through stirring linkage; It is characterized in that, also comprise dividing plate and diversion fan, said dividing plate is arranged between agitating device loam cake and the agitating device lower cover; Stirring linkage is arranged between said dividing plate and the said agitating device loam cake; Said motor is arranged between said dividing plate and the said agitating device lower cover, and said dividing plate and said agitating device loam cake form an airflow layer, and the wind-force of said diversion fan forms airflow layer and device external environment cross-ventilation.
2. high accuracy stirring at low speed system low in calories as claimed in claim 1 is characterized in that, through hole on is set on the said dividing plate, covers under the said agitating device lower through-hole is set, and said diversion fan is arranged on said going up between through hole and lower through-hole.
3. according to claim 1 or claim 2 high accuracy stirring at low speed system low in calories is characterized in that, stirs linkage and comprises at least one magnet steel frame, and said magnet steel frame is connected with said motor, and said stirrer passes through the magnetic force coupled drive with the stirring linkage.
4. high accuracy stirring at low speed system low in calories as claimed in claim 3 is characterized in that, those motors and magnet steel frame be distributed in respectively diversion fan around, said diversion fan is arranged between dividing plate and the agitating device lower cover.
5. high accuracy stirring at low speed system low in calories as claimed in claim 1; It is characterized in that; Also comprise the stirring control device; Said stirring control device comprises power supply unit, control module, interactive interface unit and I/O unit, and said power supply unit, interactive interface unit and said I/O unit are connected with said control module respectively; Also comprise some electric-motor drive units, said some electric-motor drive units are connected with said control module respectively, and said electric-motor drive unit links to each other with motor.
6. high accuracy stirring at low speed system low in calories as claimed in claim 5 is characterized in that said I/O unit comprises a text display, and said text display is connected through the Serial Port Line that intersects with said control module.
7. high accuracy stirring at low speed system low in calories as claimed in claim 5 is characterized in that said control module comprises controller, and said controller sends pulse to electric-motor drive unit.
8. high accuracy stirring at low speed system low in calories as claimed in claim 5 is characterized in that said electric-motor drive unit is a stepper motor driver, and the motor that is connected with said stepper motor driver is a stepper motor.
9. like claim 5 or 8 described high accuracy stirring at low speed systems low in calories, it is characterized in that controller is through sending pulse to stepper motor driver, the angle of the corresponding stepper motor operation of the number of pulse, the speed of the corresponding motor operation of the frequency of pulse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110183019 CN102319547B (en) | 2011-05-06 | 2011-07-01 | High-precision and low-speed stirring system with low heat quantity |
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| CN201110116784 | 2011-05-06 | ||
| CN201110116784.0 | 2011-05-06 | ||
| CN 201110183019 CN102319547B (en) | 2011-05-06 | 2011-07-01 | High-precision and low-speed stirring system with low heat quantity |
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| CN102319547A true CN102319547A (en) | 2012-01-18 |
| CN102319547B CN102319547B (en) | 2013-07-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105561851A (en) * | 2015-12-08 | 2016-05-11 | 无锡万能胶粘剂有限公司 | Dispersion machine used for adhesive production |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3199842A (en) * | 1962-04-12 | 1965-08-10 | Asea Ab | Gas-cooled electro-magnetic stirrer |
| CN2335070Y (en) * | 1997-09-18 | 1999-08-25 | 北京若比邻电子信息技术有限责任公司 | Air cooling type temp. circulation device |
| CN201313043Y (en) * | 2009-04-16 | 2009-09-23 | 上海一恒科学仪器有限公司 | Constant-temperature heating magnetic stirring apparatus |
| CN201419084Y (en) * | 2009-04-07 | 2010-03-10 | 上海森松制药设备工程有限公司 | Magnetic force stirring device for bioreactor |
-
2011
- 2011-07-01 CN CN 201110183019 patent/CN102319547B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3199842A (en) * | 1962-04-12 | 1965-08-10 | Asea Ab | Gas-cooled electro-magnetic stirrer |
| CN2335070Y (en) * | 1997-09-18 | 1999-08-25 | 北京若比邻电子信息技术有限责任公司 | Air cooling type temp. circulation device |
| CN201419084Y (en) * | 2009-04-07 | 2010-03-10 | 上海森松制药设备工程有限公司 | Magnetic force stirring device for bioreactor |
| CN201313043Y (en) * | 2009-04-16 | 2009-09-23 | 上海一恒科学仪器有限公司 | Constant-temperature heating magnetic stirring apparatus |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105561851A (en) * | 2015-12-08 | 2016-05-11 | 无锡万能胶粘剂有限公司 | Dispersion machine used for adhesive production |
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| CN102319547B (en) | 2013-07-17 |
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Effective date of registration: 20160601 Address after: Sanzao town Jinwan District Guangdong 519000 Zhuhai Province Anji Road No. 213 building A3-4 Patentee after: ZHUHAI HUABAO BIOTECHNOLOGY CO., LTD. Address before: 200237 Shanghai city Xuhui District old humin road 875 Lane 2, room 1506 Patentee before: Zhang Ming |
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Granted publication date: 20130717 Termination date: 20200701 |