CN104099235A - Small multiple fermentation system - Google Patents
Small multiple fermentation system Download PDFInfo
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- CN104099235A CN104099235A CN201310125972.9A CN201310125972A CN104099235A CN 104099235 A CN104099235 A CN 104099235A CN 201310125972 A CN201310125972 A CN 201310125972A CN 104099235 A CN104099235 A CN 104099235A
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- water
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
Abstract
The invention relates to a small multiple fermentation system. The system comprises a fermentation tank, an electromagnetic plate, a pH value controller, a water bath and a temperature controller, the fermentation tank realizes stirring under the driving of the electromagnetic plate, the pH value is adjusted through the pH value controller, and the temperature is adjusted through the temperature controller. The fermentation environment in the fermentation tank is adjusted through the pH value controller and the temperature controller, so the accurate control of the temperature, the pH value and other parameters during small scale fermentation in a laboratory; and the multiple fermentation system can also be combined with other external connection apparatuses to feed an acid, an alkali and a material liquid through different feeding inlets of the fermentation tank in an experiment process in order to maintain the feeding of the acid, the alkali and the material liquid through the different feeding inlets of the fermentation tank in the experiment process, so the constancy of the temperature, the pH value and other parameters is maintained.
Description
Technical field
The invention belongs to Bioexperiment apparatus field, especially a kind of small-sized multi-joint fermentation system.
Background technology
In microorganism fermentation test, conventionally utilize shake flask test to be optimized substratum and the preliminary study of leavening property, shake flask test has can not carry out the repeatable shortcoming such as poor of pH regulator, data, thereby in the time carrying out scale-up, often need to utilize large fermentation tank to re-start the research of medium optimization and leavening property.
Existing multi-joint fermentation equipment is used for greatly in large scale industry production, the multi-joint fermentation unit being applicable in laboratory does not almost have, required data precision is had relatively high expectations when studying for leavening property in laboratory, therefore the testing data that obtained often has relatively large deviation, is difficult to use in scale-up.So not yet find at present comparatively suitable multi-joint fermentation equipment.
Through retrieval, do not find the patent documentation relevant to teachings herein.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of and provide the small-sized multi-joint fermentation system compared with precise information for use for laboratory and the amplification culture that can be microorganism.
The object of the invention is to be achieved through the following technical solutions:
A kind of small-sized multi-joint fermentation system, comprise fermentor tank, on this fermentor tank top, lid is installed, on this lid, be shaped with multiple equipment and material socket, on this socket, be inserted with respectively pH electrode and temperature sensor, fermentor tank bottom outer wall is radially arranged on a chuck, this chuck is built-in with closed cavity and is communicated with a water-in and water outlet are installed on chuck outer wall, one magnetic rotation axis is installed in fermentor tank, also include magnetic board, pH value controller, water bath and temperature regulator, described fermentor tank is multiple and is arranged on respectively on a magnetic board, the rotation axis of installing in each ferment tank is magnetic rotation axis, on described magnetic board, be shaped with multiple magnetic agitation unit, each magnetic agitation unit can drive and be rotated the magnetic force axle in a fermentor tank respectively,
Described pH value controller comprises a casing, this casing is shaped with multiple pH electrode interfaces, pH electrode slotting on each fermentor tank is corresponding connected one by one with the pH electrode interface of pH value controller, multiple peristaltic pumps are installed on this casing, and the plurality of peristaltic pump can connect multiple external containers that acid, alkali and other feed liquids are housed;
Described water bath is shaped with many group water-bath water inlet pipes and water-bath rising pipe, every group of water-bath water inlet pipe and water-bath rising pipe respectively with the water-in of a fermentor tank external jacket and water outlet one by one corresponding independent loops be connected, this water bath heats by described circulating water heater.
And lid is run through at described rotation axis top, rotation axis is radially provided with multiple blades.
And, described each magnetic agitation unit, by a magnetic output shaft, rotational speed governor and groove or projection for solid-state fermentation tank, is shaped with the axial trough matching with magnetic output shaft shape on the chuck position of the fermentor tank bottom corresponding with magnetic output shaft.
And, on described lid, be shaped with described multiple equipment and material socket and comprise a dissolved oxygen electrode socket, a pH electrode socket, a temperature sensor socket, a material-feeding port, a benefit acid mouth, a benefit alkali mouth and a socket for subsequent use, dissolved oxygen electrode socket inserts dissolved oxygen electrode, pH electrode socket inserts described pH electrode, and temperature sensor socket inserts described temperature sensor.
And, on described pH value controller casing, indicating meter is also installed, box house is provided with micro-chip, indicating meter, multiple pH electrode interfaces and multiple peristaltic pump, multiple pH electrode interfaces are receiving after multiple pH electrode difference feedacks, send to micro-chip by transmodulator, micro-chip is crossed pilot circuit by pilot circuit by information exchange again and is transferred on indicating meter, read thus the in good time pH value in different fermentations tank, if need to the soda acid in different fermentations tank and feed liquid be augmented, micro-chip can drive respectively corresponding peristaltic pump that external container is driven into corresponding material to be specified in fermentor tank by pilot circuit.
And, signal receiver and display screen are installed on described circulating water heater, on circulating water heater, inside is provided with micro-chip, signal receiver is receiving after multiple temperature sensors difference feedacks, send to micro-chip by transmodulator, micro-chip is crossed pilot circuit by pilot circuit by information exchange again and is transferred on indicating meter.
And, between described water bath and each water-bath rising pipe, little water pump is all installed.
Advantage of the present invention and positively effect are:
1, the present invention regulates the yeasting in fermentor tank by pH value controller and temperature regulator, realize thus while fermentation on a small quantity in laboratory temperature, the isoparametric accurate control of pH value, this multi-joint fermentation system can also be by carrying out the supply of acid, alkali and feed liquid in conjunction with everfermentation tank different feeds mouth in other peripheral equipment experimentations simultaneously, remain on thus in experimentation and carry out the supply of acid, alkali and feed liquid in conjunction with everfermentation tank different feeds mouth, thereby realize temperature, pH value is isoparametric constant.
2, the present invention drives respectively the magnetic force axle in fermentor tank to stir material in tank by the many groups magnetic agitation unit on magnetic board, the rotating speed of each fermentor tank can regulate according to different situations, each fermentor tank is connected with temperature regulator with pH value controller simultaneously, has realized thus multi-joint fermentation.
3, the present invention has that easy and simple to handle, multiple fermentation tests can be carried out simultaneously, the advantage such as cost-saving, can be used for carrying out the microorganism fermentations such as bacterium, fungi, can be used in the laboratory of the factories such as medicine, biology, food or scientific research institution, and be used for replacing at present conventional shaking flask and conventional fermentation equipment.
4, the fermentation data that this use Novel small-sized fermentation equipment obtains, little with the data differences that the fermentor tank of laboratory routine obtains, leavening property and the fermentation parameter that can better understand culture, can replace large-scale fermentation equipment, and substratum and fermentation condition to microorganism are optimized.
Brief description of the drawings
Fig. 1 is the structural representation of fermentor tank in the present invention;
Fig. 2 is the vertical view of Fig. 1;
Fig. 3 be in Fig. 2 A-A to sectional view;
Fig. 4 is magnetic board structural representation in the present invention;
Fig. 5 is pH value controller structural representation in the present invention;
Fig. 6 is water bath of the present invention and circulating water heater connection diagram;
Fig. 7 is the logic diagram of pH value controller internal circuit in the present invention.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
A kind of small-sized multi-joint fermentation system, see Fig. 1 ~ 3, comprise fermentor tank 3, on this fermentor tank top, lid 1 is installed, bottom outer wall is radially arranged on a chuck 4, on lid, be shaped with respectively a dissolved oxygen electrode socket 9, a pH electrode socket 8, a temperature sensor socket 7, a material-feeding port 12, mend acid mouth 10 for one, mend alkali mouth 11 and a socket 14 for subsequent use for one, dissolved oxygen electrode socket inserts dissolved oxygen electrode (not shown), pH electrode socket inserts pH electrode (not shown), temperature sensor socket inserts temperature sensor (not shown), above-mentioned chuck is built-in with closed cavity 17 and is communicated with a water-in 5 and water outlet 6 are installed at chuck outer wall 13, one magnetic rotation axis 2 is vertically installed in fermentor tank, lid is run through at this rotation axis top, rotation axis is radially provided with multiple blades 15.
Innovative point of the present invention is:
See Fig. 4, above-mentioned fermentor tank is multiple and is arranged on respectively on a magnetic board 18, is shaped with multiple magnetic agitation unit on magnetic board, and each magnetic agitation unit can drive and be rotated the magnetic force axle in a fermentor tank respectively.
Each magnetic agitation unit, by a magnetic output shaft 19, rotational speed governor and groove or projection 20 for solid-state fermentation tank, is shaped with the axial trough 16 matching with magnetic output shaft shape on the chuck position of the fermentor tank bottom corresponding with magnetic output shaft.
See Fig. 5, also include a pH value controller, this pH value controller comprises a casing 22, this casing is shaped with multiple pH electrode interfaces 23, the plurality of pH electrode interface is corresponding connected one by one with pH electrode slotting on each fermentor tank, multiple peristaltic pumps 24 are also installed on casing, the plurality of peristaltic pump can connect multiple external containers that acid, alkali and other feed liquids are housed, under peristaltic pump drives, acid, alkali and other feed liquids are squeezed into respectively in fermentor tank by benefit acid mouth, benefit alkali mouth and material-feeding port on fermentor tank lid.
See Fig. 7, on pH value controller casing, indicating meter 21 is also installed, box house is provided with micro-chip, indicating meter, multiple pH electrode interfaces and multiple peristaltic pump, multiple pH electrode interfaces are receiving after multiple pH electrode difference feedacks, send to micro-chip by transmodulator, micro-chip is crossed pilot circuit by pilot circuit by information exchange again and is transferred on indicating meter, read thus the in good time pH value in different fermentations tank, if need to the soda acid in different fermentations tank and feed liquid be augmented, micro-chip can drive respectively corresponding peristaltic pump that external container is driven into corresponding material to be specified in fermentor tank by pilot circuit.
See Fig. 6, also comprise a water bath 25, this water bath is shaped with many group water-bath water inlet pipes 29 and water-bath rising pipe 30, every group of water-bath water inlet pipe and water-bath rising pipe respectively with the water-in of a fermentor tank external jacket and water outlet one by one corresponding independent loops be connected, this water bath heats by a circulating water heater 27, signal receiver 28 and display screen 26 are installed on this circulating water heater, on circulating water heater, inside is provided with micro-chip, signal receiver is receiving after multiple temperature sensors difference feedacks, send to micro-chip by transmodulator, micro-chip is crossed pilot circuit by pilot circuit by information exchange again and is transferred on indicating meter, read thus the in good time temperature value in different fermentations tank, if need to the temperature in different fermentations tank be adjusted, micro-chip can regulate the water temperature that refers to water bath by pilot circuit.Because this well heater is existing installation, therefore its internal circuit is not described in detail in text description of the present invention and accompanying drawing.
Between water bath and each water-bath rising pipe, little water pump (not shown) is all installed, drives thus water-bath to flow in the chuck of specifying outside fermentor tank.
Claims (7)
1. a small-sized multi-joint fermentation system, comprise fermentor tank, on this fermentor tank top, lid is installed, on this lid, be shaped with multiple equipment and material socket, on this socket, be inserted with respectively pH electrode and temperature sensor, fermentor tank bottom outer wall is radially arranged on a chuck, this chuck is built-in with closed cavity and is communicated with a water-in and water outlet are installed on chuck outer wall, one magnetic rotation axis is installed in fermentor tank, it is characterized in that: also include magnetic board, pH value controller, water bath and temperature regulator, described fermentor tank is multiple and is arranged on respectively on a magnetic board, the rotation axis of installing in each ferment tank is magnetic rotation axis, on described magnetic board, be shaped with multiple magnetic agitation unit, each magnetic agitation unit can drive and be rotated the magnetic force axle in a fermentor tank respectively,
Described pH value controller comprises a casing, this casing is shaped with multiple pH electrode interfaces, pH electrode slotting on each fermentor tank is corresponding connected one by one with the pH electrode interface of pH value controller, multiple peristaltic pumps are installed on this casing, and the plurality of peristaltic pump can connect multiple external containers that acid, alkali and other feed liquids are housed;
Described water bath is shaped with many group water-bath water inlet pipes and water-bath rising pipe, every group of water-bath water inlet pipe and water-bath rising pipe respectively with the water-in of a fermentor tank external jacket and water outlet one by one corresponding independent loops be connected, this water bath heats by described circulating water heater.
2. small-sized multi-joint fermentation system according to claim 1, is characterized in that: lid is run through at described rotation axis top, and rotation axis is radially provided with multiple blades.
3. small-sized multi-joint fermentation system according to claim 1 and 2, it is characterized in that: described each magnetic agitation unit, by a magnetic output shaft, rotational speed governor and groove or projection for solid-state fermentation tank, is shaped with the axial trough matching with magnetic output shaft shape on the chuck position of the fermentor tank bottom corresponding with magnetic output shaft.
4. small-sized multi-joint fermentation system according to claim 1 and 2, it is characterized in that: on described lid, be shaped with described multiple equipment and material socket and comprise a dissolved oxygen electrode socket, a pH electrode socket, a temperature sensor socket, a material-feeding port, a benefit acid mouth, a benefit alkali mouth and a socket for subsequent use, dissolved oxygen electrode socket inserts dissolved oxygen electrode, pH electrode socket inserts described pH electrode, and temperature sensor socket inserts described temperature sensor.
5. small-sized multi-joint fermentation system according to claim 1, it is characterized in that: on described pH value controller casing, indicating meter is also installed, box house is provided with micro-chip, indicating meter, multiple pH electrode interfaces and multiple peristaltic pump, multiple pH electrode interfaces are receiving after multiple pH electrode difference feedacks, send to micro-chip by transmodulator, micro-chip is crossed pilot circuit by pilot circuit by information exchange again and is transferred on indicating meter, read thus the in good time pH value in different fermentations tank, if need to the soda acid in different fermentations tank and feed liquid be augmented, micro-chip can drive respectively corresponding peristaltic pump that external container is driven into corresponding material to be specified in fermentor tank by pilot circuit.
6. small-sized multi-joint fermentation system according to claim 1, it is characterized in that: signal receiver and display screen are installed on described circulating water heater, on circulating water heater, inside is provided with micro-chip, signal receiver is receiving after multiple temperature sensors difference feedacks, send to micro-chip by transmodulator, micro-chip is crossed pilot circuit by pilot circuit by information exchange again and is transferred on indicating meter.
7. according to the small-sized multi-joint fermentation system described in claim 1 or 6, it is characterized in that: between described water bath and each water-bath rising pipe, little water pump is all installed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310125972.9A CN104099235A (en) | 2013-04-12 | 2013-04-12 | Small multiple fermentation system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310125972.9A CN104099235A (en) | 2013-04-12 | 2013-04-12 | Small multiple fermentation system |
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| CN104099235A true CN104099235A (en) | 2014-10-15 |
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| CN201310125972.9A Pending CN104099235A (en) | 2013-04-12 | 2013-04-12 | Small multiple fermentation system |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105334884A (en) * | 2015-11-20 | 2016-02-17 | 福建龙氟化工有限公司 | Auto-controlling device of cooling water PH value of hydrogen fluoride equipment |
| CN106754305A (en) * | 2016-11-22 | 2017-05-31 | 江南大学 | Parallel multi-joint fermentation tank |
| CN109116893A (en) * | 2018-09-30 | 2019-01-01 | 镇江华智睿安物联科技有限公司 | A kind of width Cheng Wendu is adjusted and the device of pH measurement |
| CN109401951A (en) * | 2018-11-09 | 2019-03-01 | 上海主元生物科技有限公司 | A kind of micromation high throughput installation for fermenting |
| CN110669647A (en) * | 2019-11-20 | 2020-01-10 | 佛山科学技术学院 | Laboratory microbial fermentation tank |
-
2013
- 2013-04-12 CN CN201310125972.9A patent/CN104099235A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105334884A (en) * | 2015-11-20 | 2016-02-17 | 福建龙氟化工有限公司 | Auto-controlling device of cooling water PH value of hydrogen fluoride equipment |
| CN106754305A (en) * | 2016-11-22 | 2017-05-31 | 江南大学 | Parallel multi-joint fermentation tank |
| CN109116893A (en) * | 2018-09-30 | 2019-01-01 | 镇江华智睿安物联科技有限公司 | A kind of width Cheng Wendu is adjusted and the device of pH measurement |
| CN109401951A (en) * | 2018-11-09 | 2019-03-01 | 上海主元生物科技有限公司 | A kind of micromation high throughput installation for fermenting |
| CN110669647A (en) * | 2019-11-20 | 2020-01-10 | 佛山科学技术学院 | Laboratory microbial fermentation tank |
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Application publication date: 20141015 |