CN214991613U

CN214991613U - A thallus culture apparatus for realizing genetic engineering fungus high density growth

Info

Publication number: CN214991613U
Application number: CN202120641142.1U
Authority: CN
Inventors: 赵群; 王北
Original assignee: Shandong Simcere Bio Pharmaceutical Co ltd
Current assignee: Shandong Simcere Bio Pharmaceutical Co ltd
Priority date: 2021-03-30
Filing date: 2021-03-30
Publication date: 2021-12-03
Anticipated expiration: 2031-03-30

Abstract

The utility model relates to a thallus culture apparatus for realizing genetic engineering fungus high density growth, which belongs to the technical field of microbial fermentation, the thallus culture apparatus comprises a material supplementing tank, a material supplementing pump and a fermentation tank, the material supplementing tank is connected with the inlet of the material supplementing pump through a material supplementing output pipe, and the outlet of the material supplementing pump is communicated with the fermentation tank through a fermentation inlet pipe; the upper end in the fermentation tank is provided with a feed supplement nozzle assembly, the fermentation feeding pipe is communicated with the feed supplement nozzle assembly, and the feed supplement nozzle assembly is provided with a plurality of nozzle heads; the fermentation tank is characterized in that a stirring motor is installed at the upper end of the fermentation tank, a stirring assembly is arranged in the fermentation tank, and the upper end of the stirring assembly is connected with the stirring motor. The thallus culture device can enable the culture medium, oxygen and the escherichia coli to be in contact more efficiently, high-density growth of the genetic engineering bacteria is achieved, the process is simple, the industrial cost is low, and finally large-scale and industrialization of production of target products are easier to achieve.

Description

A thallus culture apparatus for realizing genetic engineering fungus high density growth

Technical Field

The utility model relates to a thallus culture apparatus for realizing genetic engineering fungus high density growth belongs to microbial fermentation technical field.

Background

The high-density fermentation technology of the genetically engineered bacteria is the basis of the production and application of the genetically engineered bacteria, and products of the genetically engineered bacteria have great potential capability in the aspects of chemical industry, food, environmental protection, medicine and the like. But whether high-density culture and high target product concentration can be realized is a decisive factor of whether the engineering bacteria can realize large-scale production with lower cost. Therefore, various process conditions and equipment of high-density fermentation must be combined, the operation process is simplified, the industrial cost is reduced, and finally, the scale and industrialization of the production of the target product are realized.

Fermenters are mainly devices used industrially for carrying out microbial fermentations, and are also vessels used for carrying out microbial cultures. According to different properties of fermentation materials, the fermentation materials can be divided into a solid fermentation tank and a liquid fermentation tank. The fermentation cylinder main part adopts stainless steel material preparation to form, makes the material misce bene through agitating unit in the jar, and this equipment adopts the inner loop mode, smashes the foam with the stirring rake dispersion, and dissolved oxygen speed is high, and it is effectual to mix, promotes the material and carries out fermentation reaction, can realize high temperature sterilization on the throne, and the guarantee material does not receive the pollution, accords with trades such as national GMP hygienic standard, wide application in food, medicine, chemical industry.

With the rapid development of pharmaceutical and biotechnological industries, stirred tank bioreactors have received much attention due to their simple design, easy control of operating conditions and low operating costs. However, the conversion from laboratory to industrial scale has presented a significant challenge in the development of commercial processes because many of the size-related characteristics change non-linearly as the systems increase. In this case, in addition to understanding the fluid dynamics, the application of efficient scale-up methods is crucial for designing successful industrial processes.

The conventional fermentation device is used for high-density growth history of genetically engineered bacteria, and the problem of reduction of fermentation control level is caused by uneven mass concentration of local substrates due to poor mass transfer conditions.

SUMMERY OF THE UTILITY MODEL

The utility model discloses not enough to prior art exists, provide a thallus culture apparatus for realizing genetic engineering fungus high density growth, thallus culture apparatus can make culture medium, oxygen and the more efficient contact of escherichia coli, realize genetic engineering fungus's high density growth.

The utility model provides an above-mentioned technical problem's technical scheme as follows: a thallus culture device for realizing high-density growth of genetically engineered bacteria comprises a supplement tank, a supplement pump and a fermentation tank, wherein the supplement tank is connected with an inlet of the supplement pump through a supplement output pipe, and an outlet of the supplement pump is communicated with the fermentation tank through a fermentation inlet pipe;

the upper end in the fermentation tank is provided with a feed supplement nozzle assembly, the fermentation feeding pipe is communicated with the feed supplement nozzle assembly, and the feed supplement nozzle assembly is provided with a plurality of nozzle heads; the fermentation tank is characterized in that a stirring motor is installed at the upper end of the fermentation tank, a stirring assembly is arranged in the fermentation tank, and the upper end of the stirring assembly is connected with the stirring motor.

On the basis of the technical scheme, the utility model discloses can also do as follows the improvement:

further, the stirring assembly include puddler and stirring paddle leaf, the upper end of puddler is connected agitator motor, be equipped with on the puddler stirring paddle leaf, stirring paddle leaf is the heliciform, the heliciform be equipped with a plurality of disturbance baffles on the stirring paddle leaf, stirring assembly's structure stirs the culture solution, can make thalli, material, dissolved oxygen, PH, secondary metabolite etc. more even, more be favorable to the thalli to grow.

Furthermore, the feed supplement jar on be equipped with balanced pressure differential pipeline, the one end of fermentation inlet pipe extends the tank bottoms of feed supplement jar is convenient for control feed supplement jar interior external air pressure balance, can effectively prevent suck-back.

Furthermore, the upper end of the fermentation tank is provided with an air release pipe, and the air release pipe is provided with a switch valve, so that the balance of the air pressure inside and outside the fermentation tank can be controlled conveniently.

Furthermore, the fermentation tank is connected with a dissolved oxygen pipeline, and the end part of the dissolved oxygen pipeline extends to the bottom inside the fermentation tank.

Further, the support is installed to the lower extreme of fermentation cylinder, the bottom of fermentation cylinder is equipped with the evacuation pipe, be equipped with the blowoff valve on the evacuation pipe. Still be furnished with equipment such as temperature control device, temperature measurement hole, CIP autogiration spray rinsing ball, defoamer, baffle, air distributor, temperature sensor, sight glass shot-light, level gauge on the fermentation cylinder, the manual operation of being convenient for, simple reliable, fermentation cylinder connection control system can on-line measuring and control fermentation process relevant parameter. The control system comprises a sterilization interface, parameter setting and correction, a data curve graph, a data report, fermentation batch number setting, online animation, an alarm device and the like, and can be connected with an upper computer, a computer and a printer to realize remote monitoring and data storage and printing.

Furthermore, a heat exchange jacket is arranged outside the fermentation tank, a heat exchange medium inlet is formed in the lower end of the heat exchange jacket, and a heat exchange medium outlet is formed in the upper end of the heat exchange jacket, so that the temperature in the fermentation tank can be conveniently controlled.

Furthermore, the tank body of the fermentation tank is made of stainless steel, the inner surface and the outer surface of the fermentation tank are polished, the polishing precision Ra0.4 is achieved, materials can be prevented from being stuck to the wall, and the fermentation tank is easy to clean. The external connector of the fermentation tank adopts a sanitary quick-opening joint, is safe and sanitary, has no dead angle, and meets the national GMP (good manufacturing practice) sanitary standard.

Furthermore, the feed supplement pump is a peristaltic pump, feed supplement is automatically fed into the fermentation tank through the peristaltic pump, the flow rate range is 0-5L/min, the control precision is +/-2%, inoculation operation is achieved, the inoculation mode is reliable, and various inoculation modes such as flame inoculation and differential pressure inoculation can be selected.

The utility model has the advantages that: the material passes through feed supplement nozzle assembly after getting into the fermentation tank inside through the fermentation inlet pipe, forms atomizing culture medium, more can evenly add in the zymophyte liquid, and rethread heliciform stirring paddle leaf and disturbance baffle are to the stirring of culture solution, can make thalli, material, dissolved oxygen, PH, secondary metabolite etc. more even, more are favorable to the thalli to grow, make culture medium, oxygen and the more efficient contact of escherichia coli, realize the high density growth of genetic engineering fungus. By using the thallus culture device, the genetic engineering bacteria Escherichia coli can realize high-density fermentation, and the high-density fermentation can reach 160-180 g/L.

Drawings

FIG. 1 is a schematic view showing the structure of a cell culture apparatus according to the embodiment;

FIG. 2 is a schematic diagram of the operation of a feed supplement nozzle assembly;

in the figure, 1 feed tank, 2 feed pump, 3 fermentation tank, 4 feed output pipe, 5 fermentation feed pipe, 6 feed nozzle component, 7 nozzle head, 8 stirring motor, 9 stirring rod, 10 stirring paddle, 11 disturbance baffle, 12 balance pressure difference pipeline, 13 air release pipe, 14 switch valve, 15 dissolved oxygen pipeline, 16 support, 17 emptying pipe, 18 emptying valve, 19 heat exchange jacket, 20 heat exchange medium inlet, 21 heat exchange medium outlet, 22 atomized culture medium, 23 zymogen liquid.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

As shown in the figure, the thallus culture device for realizing the high-density growth of genetically engineered bacteria comprises a supplement tank 1, a supplement pump 2 and a fermentation tank 3, wherein the supplement tank 1 is connected with an inlet of the supplement pump 2 through a supplement output pipe 4, an outlet of the supplement pump 2 is communicated with the fermentation tank 3 through a fermentation inlet pipe 5, and the supplement pump 2 is a peristaltic pump;

a feed supplement nozzle assembly 6 is installed at the upper end inside the fermentation tank 3, the fermentation feeding pipe 5 is communicated with the feed supplement nozzle assembly 6, and a plurality of nozzle heads 7 are arranged on the feed supplement nozzle assembly 6; stirring motor 8 is installed to 3 upper ends of fermentation cylinder, be equipped with the stirring subassembly in the fermentation cylinder 3, the upper end of stirring subassembly is connected stirring motor 8.

Stirring subassembly include puddler 9 and stirring paddle leaf 10, the upper end of puddler 9 is connected agitator motor 8, be equipped with on the puddler 9 stirring paddle leaf 10, stirring paddle leaf 10 is the heliciform, the heliciform be equipped with a plurality of disturbance baffles 11 on the stirring paddle leaf 10.

The feed supplement tank 1 is provided with a balanced pressure difference pipeline 12, and one end of the fermentation inlet pipe 5 extends to the bottom of the feed supplement tank 1. An air release pipe 13 is arranged at the upper end of the fermentation tank 3, and a switch valve 14 is arranged on the air release pipe 13. The fermentation tank 3 is connected with a dissolved oxygen pipeline 15, and the end part of the dissolved oxygen pipeline 15 extends to the bottom in the fermentation tank 3.

The lower extreme of fermentation cylinder 3 is installed support 16, the bottom of fermentation cylinder 3 is equipped with evacuation pipe 17, be equipped with the blowoff valve 18 on the evacuation pipe 17. The fermentation tank 3 is externally provided with a heat exchange jacket 19, the lower end of the heat exchange jacket 19 is provided with a heat exchange medium inlet 20, and the upper end of the heat exchange jacket 19 is provided with a heat exchange medium outlet 21. The tank body of the fermentation tank 3 is made of stainless steel, the inner surface and the outer surface of the fermentation tank are polished, and the polishing precision Ra0.4 is achieved.

The material gets into fermentation cylinder 3 inside back through feed supplement nozzle assembly 6 through fermentation inlet pipe 5, forms atomizing culture medium 22, more can evenly add in zymophyte liquid 23, rethread heliciform stirring paddle leaf 10 and disturbance baffle 11 are to the stirring of culture solution, can make thalli, material, dissolved oxygen, PH, secondary metabolite etc. more even, more be favorable to the thalli to grow, make culture medium, oxygen and the more efficient contact of escherichia coli, realize the high-density growth of genetic engineering fungus.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A thallus culture device for realizing high-density growth of genetically engineered bacteria is characterized by comprising a supplement tank (1), a supplement pump (2) and a fermentation tank (3), wherein the supplement tank (1) is connected with an inlet of the supplement pump (2) through a supplement output pipe (4), and an outlet of the supplement pump (2) is communicated with the fermentation tank (3) through a fermentation feed pipe (5);

a material supplementing nozzle assembly (6) is installed at the upper end inside the fermentation tank (3), the fermentation feeding pipe (5) is communicated with the material supplementing nozzle assembly (6), and a plurality of nozzle heads (7) are arranged on the material supplementing nozzle assembly (6); agitator motor (8) are installed to fermentation cylinder (3) upper end, be equipped with the stirring subassembly in fermentation cylinder (3), the upper end of stirring subassembly is connected agitator motor (8).

2. The thallus culture device for realizing the high-density growth of genetically engineered bacteria according to claim 1, wherein the stirring assembly comprises a stirring rod (9) and stirring blades (10), the upper end of the stirring rod (9) is connected with the stirring motor (8), the stirring blades (10) are arranged on the stirring rod (9), the stirring blades (10) are spiral, and a plurality of disturbance baffles (11) are arranged on the spiral stirring blades (10).

3. The thallus culture device for realizing the high-density growth of genetically engineered bacteria according to claim 1, characterized in that the replenishing tank (1) is provided with a pressure difference balancing pipeline (12), and one end of the fermentation feeding pipe (5) extends to the bottom of the replenishing tank (1).

4. The device for culturing the thalli for realizing the high-density growth of the genetically engineered bacteria according to claim 1, wherein an air release pipe (13) is arranged at the upper end of the fermentation tank (3), and a switch valve (14) is arranged on the air release pipe (13).

5. The device for culturing the thallus for realizing the high-density growth of the genetically engineered bacteria according to claim 1, wherein a dissolved oxygen pipeline (15) is connected to the fermentation tank (3), and the end of the dissolved oxygen pipeline (15) extends to the bottom inside the fermentation tank (3).

6. The thallus culture device for realizing the high-density growth of the genetically engineered bacteria according to claim 1, characterized in that a bracket (16) is installed at the lower end of the fermentation tank (3), an emptying pipe (17) is arranged at the bottom of the fermentation tank (3), and an emptying valve (18) is arranged on the emptying pipe (17).

7. The device for culturing the thalli for realizing the high-density growth of the genetically engineered bacteria according to claim 1, wherein a heat exchange jacket (19) is arranged outside the fermentation tank (3), a heat exchange medium inlet (20) is arranged at the lower end of the heat exchange jacket (19), and a heat exchange medium outlet (21) is arranged at the upper end of the heat exchange jacket (19).

8. The thallus culture device for realizing the high-density growth of the genetically engineered bacteria according to claim 1, characterized in that the tank body of the fermentation tank (3) is made of stainless steel, the inner and outer surfaces are polished with a polishing precision Ra0.4.

9. The device for culturing the thallus for realizing the high-density growth of the genetically engineered bacteria according to claim 1, characterized in that the feeding pump (2) is a peristaltic pump.